CN103397076A - Application of chlorate in preparation of biochemical reagent for eliminating negative value or zero value in determination result - Google Patents
Application of chlorate in preparation of biochemical reagent for eliminating negative value or zero value in determination result Download PDFInfo
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Abstract
The invention discloses an application of chlorate in preparation of a biochemical reagent for eliminating a negative value or a zero value in a determination result. According to a novel use of the chlorate, the chlorate capable of providing a proper preservation and reaction environment to a to-be-determined object is added in the biochemical reagent, so that the to-be-determined object presents deserved biological activities, and the native conformation of the to-be-determined object is maintained; and the constitution of the to-be-determined object or a tool enzyme active center is participated, the normal operation of a reaction is guaranteed, a real detection result is obtained, and a phenomenon of the negative value or the zero value in the sample detection result is eliminated.
Description
Technical field
The invention belongs to field of biological detection, specifically, relate to chlorate and eliminate application in the biochemical reagents that negative value or null value appear in measurement result in preparation.
Background technology
Instrument for detection of biochemical indicator in body mainly contains high performance liquid chromatography and the detection of full-automatic biochemical detecting instrument at present.Detecting with the high performance liquid chromatograph device is the detection method of generally acknowledging, detected result is accurate, but because the method working method is loaded down with trivial details, the sample preparation time is long and need the instrument of usage comparison costliness to do support.Along with the development and perfection of modern zymetology and automatic clinical chemistry analyzer, increasing disease marker can be detected by the design biochemical reagents.Automatic clinical chemistry analyzer is to measure the instrument of certain specific chemical composition in body fluid according to the photoelectric colorimetry principle.As to creatinine, TOTAL BILE ACID, adenosine deaminase detection etc.Biochemical reagents are to utilize by a series of enzymatic reaction in process for preparation, make the chemical composition of appointment in determinand and color development system form hazardous substance, and this material obtains the value of determinand in sample to the interpretation of colour developing by full automatic biochemical apparatus., because the automatic clinical chemistry analyzer measuring speed is fast, accuracy is high, the consumption amount of reagent is little, now in situation of all-level hospitals, quarantine station, birth control service station and laboratory, be used widely.And in body fluid, some determinand and toolenzyme are the biomacromolecules with certain activity, therefore need provide suitable preservation and reaction environment, can show due biologic activity, thereby can accurately measure the concentration of specifying chemical composition in determinand.Present stage is to the control of various compositions and condition in the biochemical reagents Focal point and difficult point of biochemical reagents research and development just.Therefore could improve the accuracy of the result that automatic clinical chemistry analyzer measures to continuing to optimize of biochemical reagents.
Present biochemical reagents can be united automatic clinical chemistry analyzer and be detected chemical compositions most in body, but clinically, some special patient due to self physiological reason or clinical application after, during chemical composition in detecting these patient's bodies, usually result shows that detected chemical composition is shown as the illusion of zero or negative value, according to medical science general knowledge or more senior, measuring method such as high-efficient liquid phase technique more accurately, measurement result show the measured value of detected chemical composition be on the occasion of.Be direct energy supply material in human body as glucose, it keeps certain level in blood, guarantees the normal functional activity of each histoorgan of human body.Therefore must contain the glucose of finite concentration level in the humans and animals body, can not null value or negative value.Present biochemical reagents can not Accurate Determining part sufferer in the concentration of kind chemical composition in body.Therefore how eliminating the automatic clinical chemistry analyzer measurement result occurs that null value or negative value phenomenon are difficult points in the art.
Summary of the invention
Technical problem to be solved by this invention is to provide chlorate to eliminate application in the biochemical reagents that negative value or null value appear in measurement result in preparation for special sufferer, the new purposes of this chlorate is by add chlorate in biochemical reagents, the chlorate that adds can provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real detected result, has eliminated to measure the sample result and the phenomenon of null value or negative value occurs.
The present invention solves the problems of the technologies described above the technical scheme that adopts:
Chlorate is eliminated application in the biochemical reagents that negative value or null value appear in measurement result in preparation, add chlorate in biochemical reagents, formed through adjustment pH, constant volume, the last toolenzyme that adds again the biochemical reagents product that occurs null value or negative value in elimination biochemical reagents mensuration during biochemical reagents are prepared.due to special sufferer, particular matter that can secretion in body after self or drug administration can together be blended in body fluid, the particular matter that biochemical substances in determinand is secreted by sufferer is surrounded, when the biochemical reagents with not adding chlorate detect the body fluid of such sufferer, enzyme in biochemical reagents can intercept by secreted material, the biological activity of enzyme can not get performance, thereby affected the mensuration of biochemical reagents to the chemical substance of appointment in determinand, biochemical reagents there will be the phenomenon of negative value or null value together with the result of the chemical substance concentration of appointment in biochemical measurement Instrument measuring determinand.But in the survey thing, the chemical substance of appointment such as glucose or creatinine etc. exist in vivo constantly, and concentration can not be negative value or null value.After the chlorate that adds in biochemical reagents, the metal ion in chlorate can activate the enzyme in biochemical reagents, promotes carrying out smoothly of enzymatic reaction.Cl in the while chlorate
-Ion,, as passivator, destroy the particular matter of sufferer self secretion, removes the protectiveness passive film of the particular matter formation of sufferer secretion; Can keep simultaneously the structure of specifying biochemical substances in determinand, avoid specifying in determinand the degraded of biochemical substances.Make each components of biochemical reagents can be smoothly with determinand in all specify chemical substances to carry out a series of reaction, form related thing, this association thing can accurately be detected by the biochemical measurement instrument, can know the concentration of the biochemical substances of appointment in determinand according to detected result.Metal ion in the chlorate that adds has two aspects to the effect of enzyme.The one, as the cofactor of enzyme, the 2nd, as activator.The k that has as activator
+, Na
+, Mg
2+, Zn
2+, Fe
3+And Ca
2+Plasma.Chlorate can be eliminated the biochemical reagents measurement result and the phenomenon of negative value or null value occur.
Add chlorate in biochemical reagents, can use the biochemical measurement instrument to detect accurately the concentration of designated substance in thing to be checked, eliminate the illusion that occurs null value or negative value in biochemical reagents mensuration.Add chlorate in biochemical reagents, chlorate can provide suitable preservation and reaction environment for determinand, makes determinand show due biologic activity, has kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real detected result, has solved to measure the sample result and the phenomenon of null value or negative value occurs.Toolenzyme refers to the auxiliary enzymes or the indicator enzyme that use in this reagent, as using in sarcosine oxidase method reagent: sarcosine oxidase, creatine amidino groups lytic enzyme, peroxidase etc., be referred to as toolenzyme, and they are different in different reagent, differ and be decided to be same enzyme, because it has biological activity, therefore can other preparation of raw material be completed in reagent after, add finally.The biochemical reagents of biochemical reagents can be divided into single reagent biochemical reagents and double reagent biochemical reagents, are divided into R1 reagent biochemical reagents and R2 reagent biochemical reagents in the double reagent biochemical reagents.When biochemical reagents are single reagent, add chlorate in the single reagent biochemical reagents, when biochemical reagents are double reagent, add chlorate in R1 or any one reagent biochemical reagents of R2.Perhaps simultaneously at R1 or R2, add chlorate.
Described chlorate is NaCl or MgCl
2Or CaCl
2NaCl, MgCl
2, CaCl
2The salts substances that the three exists in human body or in animal body usually, select these three kinds of salts to provide suitable preservation and reaction environment for determinand preferably, make determinand show due biologic activity, keep the natural structure of determinand, and participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real detected result, has solved to measure the sample result and the phenomenon of null value or negative value occurs.In three kinds of chlorates, NaCl is optimized choice, NaCl usually is used in life, easily obtain, as for the preparation of physiological saline, NaCl can finely keep and adapt to the interior environment in body in body, in like manner NaCl also can be good at more preferably serum determinand suitable preservation and reaction environment is provided, and makes determinand show due biologic activity, has kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real detected result, has eliminated to measure the sample result and the phenomenon of null value or negative value occurs.
The concentration of described chlorate is 1-50 g/L.Can well eliminate mensuration sample result when the concentration of chlorate in the scope of 1-50 g/L and the phenomenon of null value or negative value occur.When the concentration of chlorate less than 1g/L, the too small deficiency of concentration thinks that determinand provides suitable preservation and reaction environment, determinand can not show due biologic activity, life that can only recuperation section is active, the phenomenon of null value or negative value occurs although can eliminate mensuration sample result, institute's measurement result can not reflect the concentration of specifying biological in determinand really.Greater than 50 g/L the time, too high concentration can cause disadvantageous effect to preservation and the reaction environment that determinand is carried on the contrary, thereby the due biologic activity of determinand is had slight restraining effect when the concentration of chlorate.The phenomenon of null value or negative value occurs although can eliminate mensuration sample result, institute's measurement result can not reflect the concentration of specifying biological in determinand really.
Described biochemical reagents comprise the biochemical reagents of creatinine assay, the biochemical reagents of tolal bile acid determination and the biochemical reagents that adenosine deaminase is measured.The biochemical reagents of creatinine assay are in order to detect the concentration of creatinine in determinand, the biochemical reagents of tolal bile acid determination are in order to detect the concentration of TOTAL BILE ACID in determinand, and the biochemical reagents that adenosine deaminase is measured are in order to detect the concentration of adenosine deaminase in determinand.In addition, as need other biochemical indicators in human body or in animal body, can configure the biochemical reagents of corresponding different biochemical measurement.Add chlorate in different biochemical reagents, all can eliminate the biochemical reagents measurement result and the phenomenon of negative value or null value occur.
Described NaCl concentration of at the creatinine assay biochemical reagents, adding is 1 ~ 10 g/L.Physico-chemical property according to creatinine self, the NaCl concentration of adding in the biochemical reagents of creatinine assay is 1 ~ 10 g/L, the NaCl of this concentration can better provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real creatinine detected result, has eliminated to measure the sample result and the phenomenon of null value or negative value occurs.
Described NaCl concentration of at the tolal bile acid determination biochemical reagents, adding is 1 ~ 15 g/L.Physico-chemical property according to TOTAL BILE ACID, the NaCl concentration of adding in the tolal bile acid determination biochemical reagents is 1 ~ 15 g/L, the NaCl of this concentration can better provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real TOTAL BILE ACID detected result, has solved to measure the sample result and the phenomenon of null value or negative value occurs.
Described NaCl concentration of at adenosine deaminase mensuration biochemical reagents, adding is 10 ~ 50 g/L.Physico-chemical property according to adenosine deaminase, measuring the NaCl concentration of adding in biochemical reagents at adenosine deaminase is 10 ~ 50 g/L, the NaCl of this concentration can better provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real adenosine deaminase detected result, has solved to measure the sample result and the phenomenon of null value or negative value occurs.
Described NaCl concentration of at the creatinine assay biochemical reagents, adding is 3 g/L.The concentration that the creatinine assay biochemical reagents are joined middle interpolation NaCl is that 3 g/L are optimized choice, when the concentration of adding NaCl is 3 g/L, can make determinand show due biologic activity better for determinand provides suitable preservation and reaction environment, keep the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real creatinine detected result, has solved to measure the sample result and the phenomenon of null value or negative value occurs.
Described NaCl concentration of at the tolal bile acid determination biochemical reagents, adding is 9 g/L.The concentration of adding NaCl in the tolal bile acid determination biochemical reagents is that 9 g/L are optimized choice, when the concentration of adding NaCl can better provide suitable preservation and reaction environment for determinand for 9 g/L, make determinand show due biologic activity, kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, and guaranteed the concentration detected result that normally obtains true TOTAL BILE ACID of reaction, solved and measured the sample result and the phenomenon of null value or negative value occurs.
Described NaCl concentration of at adenosine deaminase mensuration biochemical reagents, adding is 30 g/L.It is that 30 g/L are selections of an optimization that adenosine deaminase is measured the NaCl concentration of adding in biochemical reagents, when the NaCl concentration of adding is 30 g/L, can better for determinand, provide suitable preservation and reaction environment, make determinand show due biologic activity, kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real adenosine deaminase concentration detected result, has solved to measure the sample result and the phenomenon of null value or negative value occurs
In sum, the invention has the beneficial effects as follows:
Provide chlorate to eliminate application in the biochemical reagents that negative value or null value appear in measurement result in preparation for special sufferer, the new purposes of this chlorate is by add chlorate in biochemical reagents, the chlorate that adds can provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real detected result, has eliminated to measure the sample result and the phenomenon of null value or negative value occurs.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited only to this.
Embodiment 1:
Chlorate is eliminated application in the biochemical reagents that negative value or null value appear in measurement result in preparation.Add chlorate in biochemical reagents, chlorate can provide suitable preservation and reaction environment for determinand, makes determinand show due biologic activity, has kept the native conformation of determinand; And participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real detected result, has eliminated to measure the sample result and the phenomenon of null value or negative value occurs.
Embodiment 2:
Chlorate is eliminated application in the biochemical reagents that negative value or null value appear in measurement result in preparation, adds chlorate in the layoutprocedure of biochemical reagents.Described chlorate is NaCl or MgCl
2Or CaCl
2NaCl, MgCl
2, CaCl
2Three's concentration is 1-50 g/L.Biochemical reagents comprise the biochemical reagents of creatinine assay, the biochemical reagents of tolal bile acid determination and the biochemical reagents configuration that adenosine deaminase is measured.NaCl, MgCl
2, CaCl
2The salts substances that the three exists in human body or in animal body usually, select these three kinds of salts to provide suitable preservation and reaction environment for determinand, make determinand show due biologic activity, keep the natural structure of determinand, and participated in the formation in test substance or toolenzyme active centre, what guaranteed reaction normally obtains real detected result, has solved to measure the sample result and the phenomenon of null value or negative value occurs.As NaCl, MgCl
2, CaCl
2Three's concentration can well be eliminated mensuration sample result and the phenomenon of null value or negative value occur in the scope of 1-50 g/L.As NaCl, MgCl
2, CaCl
2Three's concentration is less than 1g/L, and the too small deficiency of concentration thinks that determinand provides suitable preservation and reaction environment, makes determinand can not show due biologic activity.As NaCl, MgCl
2, CaCl
2Three's concentration is greater than 50 g/L the time, and too high concentration can cause slight disadvantageous effect to preservation and the reaction environment that determinand is carried on the contrary, thereby suppresses the due biologic activity of part determinand.
Embodiment 3:
Measure the concentration of sufferer creatinine in serum with the biochemical reagents of creatinine assay, these biochemical reagents are double reagent, and double reagent is respectively R1 and R2, adds NaCl in the R1 in double reagent, and the concentration of NaCl is 1-10g/L, and detailed process is:
the process of configuration R1 reagent biochemical reagents is, add approximately 700ml purified water (the purified water quality meets the requirement of external diagnosis reagent water) in the clean beaker of 1000ml, then the HEPES that takes 50 mmol/L is 2-[4-(2-hydroxyethyl)-1-piperazine] ethyl sulfonic acid 11.92 g, add stirring and dissolving in beaker, then the tensio-active agent TX-100 that adds 1 ml is Triton-100, stirring and dissolving, adding TOOS is N-ethyl-N-(2-hydroxyl-3-sulfopropyl again)-3-monomethylaniline 0.99 g, add 1-10g NaCl, stirring and dissolving mixes, NaOH initial adjustment reagent pH to 7.6 with 20%, be settled to 1000 ml, add again sarcosine oxidase 10 ku, creatine amidino groups lytic enzyme 50 ku,
The process of configuration R2 reagent biochemical reagents is, add approximately 700ml purified water in the clean beaker of 1000ml, purified water quality symbol needs fit outer diagnostic reagent water requirement, then take the 2-[4-(2-hydroxyethyl) of 50 mmol/L-1-piperazine] ethyl sulfonic acid 11.92 g, add stirring and dissolving in beaker, then regulate pH to 7.6 ± 0.1 formation damping fluid with 20% NaOH, adding 4-AAP is 4-AA 0.61g stirring and dissolving again, be settled to 1000ml, add finally peroxidase 8ku, add creatinine amidohydrolase 250ku;
Analyze mensuration with full-automatic or semi-automatic biochemical analyzer, location parameter is as follows:
Method: the end-point method sample reagent is than being sample: R1:R2=5:225:75
Measure wavelength: 37 ℃ of reaction times of 546nm temperature of reaction: 10 min.
Embodiment 4:
Detect the concentration of TOTAL BILE ACID in sufferer serum with the biochemical reagents of tolal bile acid determination.These biochemical reagents are double reagent, and double reagent is respectively R1 and R2, and adding simultaneously concentration in the R1 in double reagent and R2 is 1 ~ 15 g/L NaCl, and detailed process is:
the process of configuration R1 reagent biochemical reagents is, add approximately 700ml purified water in the clean beaker of 1000ml, the purified water quality need meet the requirement of external diagnosis reagent water, then the CAPS that takes 50 mmol/L is that N-cyclohexyl-Homotaurine is 12.97g, add stirring and dissolving in beaker, then transfer pH to 5.05 ± 0.05 to form damping fluid with HCL, adding tensio-active agent TX-100 is Triton-100 1 ml stirring and dissolving again, add again NaCl 9 g, and the Thio-NAD of 1g is Thio-NAD, stirring and dissolving mixes, be settled to 1000ml,
the layoutprocedure of configuration R2 reagent biochemical reagents, add approximately 700ml purified water in the clean beaker of 1000ml, the purified water quality need meet the requirement of external diagnosis reagent water, then the Tris that takes 100mmol/L is Tutofusin tris 12.11g, add stirring and dissolving in beaker, then taking 60 mmol/L TAPS is N-three (methylol) methyl-3-aminopropanesulfonicacid acid 14.60g, stirring and evenly mixing, adding 1ml tensio-active agent TX-100 is Triton-100 again, add NaCl 9g, add bovine serum albumin 5g, stirring and dissolving mixes, after be settled to 1000ml.Adding 3 α-HSD is that 3-α-steroid dehydrogenase 6ku and β-NADH are reduced form acid amides adenine dinucleotide 6g again;
Analyze mensuration with full-automatic or semi-automatic biochemical analyzer, location parameter is as follows:
Method: the volume ratio of rate method sample reagent is, sample: R1:R2=:5:225:75
Measure wavelength: 37 ℃ of reaction times of 405nm temperature of reaction: 10 min.
Embodiment 5:
Measure the concentration of adenosine deaminase with the biochemical reagents that adenosine deaminase is measured.These biochemical reagents are double reagent, and double reagent is respectively R1 and R2, and adding simultaneously concentration in the process for preparation of the R2 in double reagent is 10 ~ 50 g/L NaCl, and detailed process is:
Configuration R1 reagent biochemical reagents layoutprocedure, add approximately 700ml purified water in the clean beaker of 1000ml, the purified water quality need meet the requirement of external diagnosis reagent water, then the K2HPO4 that takes 50 mmol/L is dipotassium hydrogen phosphate 11.41g, add stirring and dissolving in beaker, then to add the NaH2PO4 of 30mmol/L be that SODIUM PHOSPHATE, MONOBASIC 3.60g forms damping fluid, then adding EDTA-2Na is disodium ethylene diamine tetraacetate 1.86g, mix dissolving and mix, be settled to 1000ml.Adding POD is peroxidase 5ku again, and adding XOD is XOD 5ku, and adding PNP is that purine nucleoside phosphorylase 5ku and ASOM are Vitamin C oxidase 10ku;
the layoutprocedure of configuration R2 reagent biochemical reagents is, add approximately 700ml purified water in the clean beaker of 1000ml, the purified water quality need meet the requirement of external diagnosis reagent water, then the MES that takes 50 mmol/L is MES 9.76g, add stirring and dissolving in beaker, NaOH with 20% transfers PH to 6.5 ± 0.05 to form damping fluid, then take adenosine 5.35g, stirring and evenly mixing, add again NaCl 30g, and TOOS is N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-methylbenzene amine salt 0.99g, stirring and dissolving mixes, after be settled to 1000ml,
Analyze mensuration with full-automatic or semi-automatic biochemical analyzer, location parameter is as follows:
Method: the volume ratio of rate method sample reagent is, sample: R1:R2=5:225:75
Measure wavelength: 37 ℃ of reaction times of 546nm temperature of reaction: 10 min.
Embodiment 6:
With the glucose concn in the biochemical reagents mensuration sufferer serum of glucose assays, these biochemical reagents are single reagent, and adding concentration in reagent is 1 ~ 15 g/L NaCl, and detailed process is:
Configuration R reagent biochemical reagents, add approximately 700ml purified water in the clean beaker of 1000ml, the purified water quality need meet the requirement of external diagnosis reagent water, then taking 50 mmol/L MES is MES 9.76g, add stirring and dissolving in beaker, NaOH with 20% transfers pH to 6.5 ± 0.1 to form damping fluid, the tensio-active agent TX-100 that adds 1ml is Triton-100, adding 4-AAP is 4-AA 0.11g, and phenol 0.19g, 8 g NaCl, stirring and evenly mixing, be settled to 1000ml.Adding GOD is glucose oxidase 28ku again, and adding POD is peroxidase 5ku;
Analyze mensuration with full-automatic or semi-automatic biochemical analyzer, location parameter is as follows:
Method: the volume ratio of end-point method sample reagent is, sample: R1=3:300
Measure wavelength: 505 37 ℃ of the nm temperature of reaction reaction times: 10 min.
The beneficial effect of below through detected result explanation biochemical reagents of the present invention, bringing into play:
Detect in 10 different sufferers the concentration of creatinine in the serum sample to be tested, 10 kinds of different serum testing samples, 3 parts of every kind of testing samples, every kind of testing sample detects respectively the concentration of creatinine in testing sample with 3 kinds of modes, 3 kinds of modes are respectively: sophisticated method HPLC be in the high performance liquid chromatography test sample creatine concentration, be added with the creatine concentration in the creatinine assay biochemical reagents associatings Biochemical Analyzer test sample of NaCl, and with the creatinine assay biochemical reagents that do not add NaCl, unite creatine concentration in the Biochemical Analyzer test sample.The biochemical reagents that are added with NaCl are that interpolation concentration is the NaCl of 1 ~ 50 g/L in the biochemical reagents that do not add NaCl.3 kinds of modes are measured in sufferer blood creatine concentration, and correlation data is in Table 1 as a result, and in table, data unit is μ mol/L:
In human muscular tissue, creatine mainly forms creatinine lentamente by irreversible non-enzyme dehydration reaction, then is discharged in blood, then by glomerular filtration with homaluria.According to medical science general knowledge, contain certain density creatinine in blood plasma in human body or animal body, can not be negative value or null value.It is that the concentration results that the biochemical reagents of the NaCl of 1 ~ 50 g/L are measured the creatinine in 10 serum samples of sufferer can be found out that use as shown in table 1 is added with concentration, detect the result of gained with the biochemical reagents that are added with NaCl in the present invention very close with result that high performance liquid chromatography is measured, the detected result accuracy is high, can replace high performance liquid chromatography.With the creatine concentration in the biochemical reagents test sample of not adding NaCl, negative value or null value appear in the result that obtains that detects.Show that in biochemical reagents adding NaCl can eliminate biochemical reagents and occur the phenomenon of negative value or null value in detected result.
The testing process of all conditions such as above-mentioned creatine concentration, the creatinine assay biochemical reagents that are added with NaCl are that interpolation concentration is the NaCl of 0.5 g/L in the biochemical reagents that do not add NaCl, 3 kinds of modes are measured in sufferer blood creatine concentration, and correlation data is following in Table 2 as a result, and in table, data unit is μ mol/L::
It is that the concentration results that the biochemical reagents of the NaCl of 0.5 g/L are measured the creatinine in 10 serum samples of sufferer can be found out that use as shown in table 2 is added with concentration, with the creatine concentration in the biochemical reagents test sample of not adding NaCl, negative value or null value appear in the result that obtains that detects.And the concentration results that detects gained with the biochemical reagents that are added with NaCl in the present invention is all greater than zero, shows that adding NaCl in biochemical reagents can eliminate biochemical reagents and occur the phenomenon of negative value or null value in detected result.But the result that the creatine concentration at the biochemical reagents of the NaCl that is added with concentration 0.5 g/L in test sample and high performance liquid chromatography are measured differs larger.When the concentration of chlorate less than 1g/L, the too small deficiency of concentration thinks that determinand provides suitable preservation and reaction environment, determinand can not show due biologic activity, life that can only recuperation section is active, the phenomenon of null value or negative value occurs although can eliminate mensuration sample result, institute's measurement result can not reflect the concentration of specifying biological in determinand really.
The testing process of all conditions such as above-mentioned creatine concentration, the creatinine assay biochemical reagents that are added with NaCl are that interpolation concentration is the NaCl of 80 g/L in the biochemical reagents that do not add NaCl, 3 kinds of modes are measured in sufferer blood creatine concentration, and correlation data is following in Table 3 as a result, and in table, data unit is μ mol/L::
It is that the concentration results that the biochemical reagents of the NaCl of 80 g/L are measured the creatinine in 10 serum samples of sufferer can be found out that use as shown in table 3 is added with concentration, with the creatine concentration in the biochemical reagents test sample of not adding NaCl, negative value or null value appear in the result that obtains that detects.And the concentration results that detects gained with the biochemical reagents that are added with NaCl in the present invention is all greater than zero, shows that adding NaCl in biochemical reagents can eliminate biochemical reagents and occur the phenomenon of negative value or null value in detected result.But the result that the creatine concentration at the biochemical reagents of the NaCl that is added with concentration 80 g/L in test sample and high performance liquid chromatography are measured differs larger.Greater than 50 g/L the time, too high concentration can cause disadvantageous effect to preservation and the reaction environment that determinand is carried on the contrary, thereby the due biologic activity of determinand is had slight restraining effect when the concentration of chlorate.The phenomenon of null value or negative value occurs although can eliminate mensuration sample result, institute's measurement result can not reflect the concentration of specifying biological in determinand really.
Detect in 10 different sufferers the concentration of TOTAL BILE ACID in the serum sample to be tested, 10 kinds of different serum testing samples, 3 parts of every kind of testing samples, every kind of testing sample detects respectively the concentration of TOTAL BILE ACID in testing sample with 3 kinds of modes, 3 kinds of modes are respectively: sophisticated method HPLC be in the high performance liquid chromatography test sample concentration of total bile acid, with the concentration of total bile acid in reagent of the present invention associating Biochemical Analyzer test sample, and with the concentration of total bile acid in general reagent associating Biochemical Analyzer test sample.To add in the layoutprocedure of general reagent that to add concentration be the NaCl of 1 ~ 15g/L with reagent of the present invention.3 kinds of modes are measured in sufferer blood concentration of total bile acid, and correlation data is in Table 4 as a result, and in table, data unit is μ mol/L:
In human body, TOTAL BILE ACID is always all in being discharged into blood slowly.According to medical science general knowledge, contain certain density TOTAL BILE ACID in blood plasma in human body or animal body, can not be negative value or null value.It is that the concentration results that the biochemical reagents of the NaCl of 1 ~ 15 g/L are measured the TOTAL BILE ACID in 10 serum samples of sufferer can be found out that use as shown in table 4 is added with concentration, detect the result of gained with the biochemical reagents that are added with NaCl in the present invention very close with result that high performance liquid chromatography is measured, the detected result accuracy is high, can replace high performance liquid chromatography.With the concentration of total bile acid in the biochemical reagents test sample of not adding NaCl, negative value or null value appear in the result that obtains that detects.Show that in biochemical reagents adding NaCl can eliminate biochemical reagents and occur the phenomenon of negative value or null value in detected result.
Detect in 10 different sufferers the concentration of adenosine deaminase in the serum sample to be tested, 10 kinds of different serum testing samples, 3 parts of every kind of testing samples, every kind of testing sample detects respectively the concentration of adenosine deaminase in testing sample with 3 kinds of modes, 3 kinds of modes are respectively: sophisticated method HPLC be in the high performance liquid chromatography test sample adenosine deaminase concentration, with the adenosine deaminase concentration in reagent of the present invention associating Biochemical Analyzer test sample, and with the adenosine deaminase concentration in general reagent associating Biochemical Analyzer test sample.To add in the layoutprocedure of general reagent that to add concentration be the NaCl of 10 ~ 50 g/L with reagent of the present invention.3 kinds of modes measure that in sufferer blood, adenosine deaminase concentration results correlation data is in Table 5, and in table, data unit is μ mol/L:
Adenosine deaminase is distributed widely in each tissue of human body,, according to medical science general knowledge, contains certain density adenosine deaminase in blood plasma in human body or animal body, can not be negative value or null value.It is that the concentration results that the biochemical reagents of the NaCl of 10 ~ 50g/L are measured the adenosine deaminase in 10 serum samples of sufferer can be found out that use as shown in table 5 is added with concentration, detect the result of gained with the biochemical reagents that are added with NaCl in the present invention very close with result that high performance liquid chromatography is measured, the detected result accuracy is high, can replace high performance liquid chromatography.With the adenosine deaminase concentration in the biochemical reagents test sample of not adding NaCl, negative value or null value appear in the result that obtains that detects.Show that in biochemical reagents adding NaCl can eliminate biochemical reagents and occur the phenomenon of negative value or null value in detected result.
Claims (10)
1. chlorate is eliminated application in the biochemical reagents that negative value or null value appear in measurement result in preparation.
2. application according to claim 1, is characterized in that, described chlorate is NaCl or MgCl
2Or CaCl
2
3. application according to claim 1, is characterized in that, the concentration of described chlorate is 1-50 g/L.
4. the described application of according to claim 1 to 3 any one, is characterized in that, described biochemical reagents comprise the biochemical reagents of creatinine assay, the biochemical reagents of tolal bile acid determination and the biochemical reagents that adenosine deaminase is measured.
5. application according to claim 4, is characterized in that, described NaCl concentration of at the creatinine assay biochemical reagents, adding is 1 ~ 10 g/L.
6. application according to claim 4, is characterized in that, described NaCl concentration of at the tolal bile acid determination biochemical reagents, adding is 1 ~ 15 g/L.
7. application according to claim 4, is characterized in that, described NaCl concentration of at adenosine deaminase mensuration biochemical reagents, adding is 10 ~ 50 g/L.
8. application according to claim 5, is characterized in that, described NaCl concentration of at the creatinine assay biochemical reagents, adding is 3 g/L.
9. application according to claim 6, is characterized in that, described NaCl concentration of at the tolal bile acid determination biochemical reagents, adding is 9 g/L.
10. application according to claim 7, is characterized in that, described NaCl concentration of at adenosine deaminase mensuration biochemical reagents, adding is 30 g/L.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105002264A (en) * | 2015-07-28 | 2015-10-28 | 中生北控生物科技股份有限公司 | Total bile acid measuring kit |
| CN107653298A (en) * | 2017-11-15 | 2018-02-02 | 浙江夸克生物科技有限公司 | Adenosine deaminase determines kit |
| CN108007922A (en) * | 2017-12-21 | 2018-05-08 | 广州市进德生物科技有限公司 | A kind of kit using luminol chemiluminescence analysis detection glucose |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105002264A (en) * | 2015-07-28 | 2015-10-28 | 中生北控生物科技股份有限公司 | Total bile acid measuring kit |
| CN105002264B (en) * | 2015-07-28 | 2017-12-15 | 中生北控生物科技股份有限公司 | A kind of tolal bile acid determination reagent box |
| CN107653298A (en) * | 2017-11-15 | 2018-02-02 | 浙江夸克生物科技有限公司 | Adenosine deaminase determines kit |
| CN108007922A (en) * | 2017-12-21 | 2018-05-08 | 广州市进德生物科技有限公司 | A kind of kit using luminol chemiluminescence analysis detection glucose |
| CN108007922B (en) * | 2017-12-21 | 2018-11-13 | 广州市进德生物科技有限公司 | A kind of kit detecting glucose using luminol chemiluminescence analysis |
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| CN103397076B (en) | 2016-02-17 |
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