CN103364344A - Integrated micro-optics structural array device - Google Patents
Integrated micro-optics structural array device Download PDFInfo
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- CN103364344A CN103364344A CN201210104693XA CN201210104693A CN103364344A CN 103364344 A CN103364344 A CN 103364344A CN 201210104693X A CN201210104693X A CN 201210104693XA CN 201210104693 A CN201210104693 A CN 201210104693A CN 103364344 A CN103364344 A CN 103364344A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B19/00—Condensers, e.g. light collectors or similar non-imaging optics
- G02B19/0004—Condensers, e.g. light collectors or similar non-imaging optics characterised by the optical means employed
- G02B19/0028—Condensers, e.g. light collectors or similar non-imaging optics characterised by the optical means employed refractive and reflective surfaces, e.g. non-imaging catadioptric systems
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B19/00—Condensers, e.g. light collectors or similar non-imaging optics
- G02B19/0033—Condensers, e.g. light collectors or similar non-imaging optics characterised by the use
- G02B19/0047—Condensers, e.g. light collectors or similar non-imaging optics characterised by the use for use with a light source
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B3/00—Simple or compound lenses
- G02B3/0006—Arrays
- G02B3/0037—Arrays characterized by the distribution or form of lenses
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- G02B5/02—Diffusing elements; Afocal elements
- G02B5/0205—Diffusing elements; Afocal elements characterised by the diffusing properties
- G02B5/021—Diffusing elements; Afocal elements characterised by the diffusing properties the diffusion taking place at the element's surface, e.g. by means of surface roughening or microprismatic structures
- G02B5/0215—Diffusing elements; Afocal elements characterised by the diffusing properties the diffusion taking place at the element's surface, e.g. by means of surface roughening or microprismatic structures the surface having a regular structure
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
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Abstract
The invention discloses an integrated micro-optics structural array device which comprises a solid base integrated with multiple groups of micro-optics structural arrays, and a frame, wherein the multiple groups of micro-optics structural arrays are distributed on one plane in an array manner; the frame is combined on the solid base and separates the multiple groups of micro-optics structural arrays. Each micro-optics structure is a light converting structure, or is formed by combining a light converting structure and a condensation structure, wherein the light converting structure is a circular truncated cone structure, a cylinder structure, a square column structure, a hexagonal column structure or an octahedral column structure, and the condensation structure is a convex lens or a Fresnel lens. The size of the device is same as that of a standard microwell plate, and the positions of the multiple groups of micro-optics structural arrays correspond to that of holes in the standard microwell plate one by one. The micro-optics structure improves the signal acquisition efficiency, the standard microwell plate structure is compatible with an existing device, and the automation of a whole detection process is realized.
Description
Technical field
The present invention relates to the biotechnology detection field, by making up micro-analysis system in the surperficial fixedly target molecules of micro optical structure, especially, relate to a kind of integrated micro-optical array of structures device.
Background technology
In the prior art, microarray technology is a kind of flat carrier, is fitly arranging many unit according to row and column above it, is adsorbing regularly, specifically gene or protein molecule in each unit.An analytical equipment is called as microarray, should meet: 1. be the plane, 2. well-regulated and 3. be three requirements on micro-scale.The micro-scale of microarray on reaction kinetics so that reaction can occur rapidly, thereby realize the fast detecting of large figureofmerit.
The present common Application standard glass slide of micro-array chip (1 inch * 3 inches), yet, use the micro-array chip of glass slide to exist following problem:
1. operate cumbersomely, for example cleaning of the stickup of chip fence, slide, and be manually-operated is unfavorable for the Stability and veracity that reacts.
2. detect dumbly, at every turn must use whole slide, if number of samples is fewer, then can cause waste.
3. the coated organic molecular film of glass surface comes off easily, causes experimental result unstable.
4. the planar structure of glass slide causes phosphor collection efficient low, and glass surface is not easy to again do chemical treatment, and the background signal of glass is high, causes it to be unfavorable for carrying out highly sensitive detection.
Summary of the invention
Fundamental purpose of the present invention is, the defective for above-mentioned prior art exists provides a kind of novel integrated micro-optical array of structures device, and it can improve the signal collection efficiency of biochip, can realize automation mechanized operation, can also strengthen the dirigibility of detection.
To achieve these goals, the present invention is by the following technical solutions:
A kind of integrated micro-optical array of structures device, wherein, it comprises solid substrate and the framework that is integrated with the micro optical structure array; Described micro optical structure array has many groups, and respectively organizes the micro optical structure array and in one plane be arranged in array; Described framework is combined on the solid substrate and with each group micro optical structure array to be separated.
Described micro optical structure has the effect of signal enhancing to signals such as fluorescence, chemiluminescence, time-resolved fluorescence, surface plasma resonances (SPR, Surface plasmon resonance).
Aforesaid integrated micro-optical array of structures device, wherein,
Described solid substrate by a plurality of structures and size all identical pore structure rearrange equally spacedly; All be integrated with one group of micro optical structure array on the bottom surface of each pore structure inside, and be combined with the reactive group that can be combined with biomolecule on the inside surface of pore structure, outside surface is provided with retaining structure;
Described framework is provided with the hole lattice of a plurality of at grade rectangular array arrangements, each hole lattice is provided with location structure, the accommodating described pore structure of difference in each hole lattice, and the retaining structure phase blocking of location structure and described pore structure outside surface, described solid substrate is fixed on the framework, and the micro optical structure array on each pore structure inner bottom surface is in the same plane.
Aforesaid integrated micro-optical array of structures device, preferably, the material of described solid substrate is a kind of in polystyrene, cyclic olefin polymer and the styrene/acrylonitrile copolymer.
Aforesaid integrated micro-optical array of structures device, preferably, described solid substrate and described micro optical structure are one machine-shaping.
Aforesaid integrated micro-optical array of structures device, wherein,
Described solid substrate is planar structure, and the described micro optical structure array of respectively organizing is integrated in the upper surface of solid substrate and rectangular array arrangement;
Described framework is incorporated into the upper surface of described solid substrate, which is provided with a plurality of through holes, the rectangular array arrangement of each through hole, and the position of described through hole and described micro optical structure array is corresponding and the micro optical structure array is come out from through hole one by one;
Upper surface in described solid-state base, the position of coming out from described through hole are combined with the reactive group that can be combined with biomolecule.
Aforesaid integrated micro-optical array of structures device, described micro optical structure and the machine-shaping of solid substrate one.
Aforesaid integrated micro-optical array of structures device, preferably,
Described framework is rigid material, with described solid substrate with combine by gluing or mechanical fixing mode; Perhaps
Described framework is elastomeric material, sticks on the upper surface of solid substrate.
Aforesaid integrated micro-optical array of structures device, preferably, the size of described integrated micro-optical array of structures device is big or small identical with the standard microwell plate, and the described position of respectively organizing the micro optical structure array is corresponding one by one with the hole on the standard microwell plate.
Aforesaid integrated micro-optical array of structures device, wherein, described standard microwell plate can be standard 384 orifice plates, 96 orifice plates, 72 orifice plates, 48 orifice plates, 8 orifice plate bars, 12 orifice plate bars, 16 orifice plate bars etc.
Aforesaid integrated micro-optical array of structures device, wherein, described micro optical structure is for turning photo structure, or forms by turning the set of photo structure and concentration structure.
Aforesaid integrated micro-optical array of structures device, preferably, the described photo structure that turns is a kind of in frustum cone structure, column structure, square column structure, six rod structures and the octagon column structure, and the described upper surface that turns photo structure is the plane.
Aforesaid integrated micro-optical array of structures device, preferably, the described outside surface that turns photo structure is coated with reflectance coating, and described reflectance coating is metallic reflective coating, a kind of in dielectric reflectance coating and the inter metal dielectric reflectance coating.
Aforesaid integrated micro-optical array of structures device, preferably, described concentration structure is a kind of in convex lens and the Fresnel Lenses, described concentration structure is integrated in the bottom that turns photo structure.
Aforesaid integrated micro-optical array of structures device, preferably, described concentration structure and described to turn photo structure one-body molded
Beneficial effect of the present invention is:
Micro optical structure array apparatus of the present invention, its micro optical structure that adopts has improved the collection efficiency of signal, and has therefore reduced the requirement of checkout equipment, and this micro optical structure can also provide the positional information of signal, simplifies time and workload that software is processed; The compatible existing equipment of its slight AND DEWATERING FOR ORIFICE STRUCTURE that adopts can make whole testing process realize automation mechanized operation, improves the Stability and veracity that detects; Its demountable structure that adopts makes detection more flexible, and every plate can be realized the detection of 1-96 sample.
Description of drawings
Fig. 1 is the one-piece construction synoptic diagram of the embodiment 1 of integrated micro-optical array of structures device of the present invention.
Fig. 2 is the longitudinal sectional view in one of them hole in the embodiment of the invention 1.
Fig. 3 be the embodiment of the invention 2 and 3 integrated micro optical structure array solid substrate overlook schematic appearance.
Fig. 4 is the synoptic diagram of arranging of the micro optical structure array of the embodiment of the invention 2.
Fig. 5 be the embodiment of the invention 2 framework with holes overlook schematic appearance.
Fig. 6 is the vertical profile synoptic diagram of a kind of framework with holes of the embodiment of the invention 2.
Fig. 7 is the vertical profile synoptic diagram of the another kind of framework with holes of the embodiment of the invention 2.
Fig. 8 is the structural representation of the grillage of the embodiment of the invention 2.
Fig. 9 be the embodiment of the invention 3 framework with holes overlook schematic appearance.
Figure 10 is the vertical profile synoptic diagram of a kind of embodiment of micro optical structure of the present invention.
Figure 11 is the vertical profile synoptic diagram of the another kind of embodiment of micro optical structure of the present invention.
Embodiment
The invention provides a kind of integrated micro-optical array of structures device, clearer, clear and definite for making purpose of the present invention, technical scheme and advantage, developing simultaneously referring to accompanying drawing, the present invention is described in more detail for embodiment.
(1) structure of integrated micro-optical array of structures device
Embodiment 1
Shown in the longitudinal sectional view of the structural representation of Fig. 1 and Fig. 2, in the present embodiment, integrated micro-optical array of structures device is comprised of with sheet frame 200 lath 100 of integrated micro optical structure array.The used ELIAS strip of ELISA (enzyme-linked immunosorbent assay) is identical in the structure of described lath 100 and the prior art, and it can be the regular sizes such as 8 holes, 12 holes, 16 holes, and can be disassembled into any hole count and place on the sheet frame.The single pore structure 110 of lath 100 can be the shape such as circular or square, and pitch of holes is identical with the pitch of holes of standard microwell plate.The inside surface 112 of each pore structure 110 is through surface treatment, has the reactive group that to be combined with biomolecule, such as the aldehyde radical that can be combined with protein sample or epoxide group etc., and the integrated array of micro optical structure 111 on the inner bottom surface, and preferably, the aspect ratio of pore structure 110 and micro optical structure 111 is greater than 100 times.Described micro optical structure 111 has the light action of turning, and can improve the collection rate of signal.The outside surface of each pore structure 110 also is provided with retaining structure 113, is used for sheet frame 200 fixing.
Described sheet frame 200 can design with reference to the specification of existing standard microwell plate, such as standard 384 orifice plates, standard 96 orifice plates, standard 48 orifice plates, 8 orifice plate bars etc.Particularly, take employing standard 96 orifice plate specifications as example, described sheet frame 200 can hold 1-96 pore structure 110, has the petal design 210 that 117 (13x9) group of arranging according to the layout (12x8) of standard 96 orifice plates is used for fixation hole configurations on it.Particularly, described sheet frame 200 comprises a housing, and its space that surrounds is separated by orthogonal 11 horizontal stripes and 7 taeniaes, forms 96 square hole lattice that distribute as net shape, and described petal design 210 is arranged on the place, four right angles of each hole lattice.Petal design on the point of crossing of horizontal stripe and taeniae is comprised of four petal elastic clips; The petal design of locating in the point of crossing of horizontal stripe or taeniae and housing is comprised of two petal elastic clips.Every elastic clip all is a cambered surface, and this cambered surface is protruded to the hole center of a lattice direction at its place, therefore is equipped with an elastic clip that goes out to cardiac prominence wherein at each hole lattice of sheet frame 200 four jiaos, a pore structure 113 can be fixed therein.
When lath 100 is placed on the sheet frame 200, four elastic clips that are arranged in each place, Kong Gesi angle are clamped in the retaining structure 113 of each pore structure 110, realize pore structure 110 fixing on sheet frame 200, thereby form the integrated micro-optical array of structures device in the present embodiment.The interaction of described elastic clip and retaining structure 113, even also can guarantee when sheet frame 200 is inverted, firmly to pat sheet frame 200, lath 100 still can be fixed on the sheet frame 200, and this good stationarity is so that this integrated micro-optical array of structures device is easy to pick and place.
In the present embodiment, the material of the lath 100 of integrated micro optical structure array can be the materials such as polystyrene, cyclic olefin polymer, styrene/acrylonitrile copolymer, and it is by the machine-shaping of Shooting Technique one.On the inner bottom surface of each pore structure 113 of lath 100 of the present invention, in the circumference of diameter 4mm, form the array of the micro optical structure of 8*8; Make that the upper surface diameter is that hundred microns micro optical structure array evenly distributes, and be on the same level face, and the height error of each micro optical structure is strict controlled in 5%.
The core rod of the injection mold of lath 100 of the present invention adopts EDM electrosparking mode, and the concrete technology route is: 1. adopt five axle machining centers engraving electrode, and electrode is processed through surface finish; 2. EDM lathe configuration microfabrication power supply guarantees size, shape, the surfaceness requirement of core rod; 3. core rod fully utilizes the process for treating surface that machinery, physics, chemistry combine at last, in the situation of not destroying size, shape, makes surfaceness reach Ra≤0.02 μ m.
Above-mentioned integrated micro-optical array of structures device is when being applied to detect, upper surface at the micro optical structure array carries out point sample, again through after the operations such as fixing, sealing, cleaning, can directly micro array apparatus be placed full-automatic enzyme-linked immunologic workstation or other general self-reacting devices that can be used for 96 orifice plates to carry out application of sample, hatch, wash the operation such as plate; Behind the EO, be placed on and carry out reading result in the specific scanner.Perhaps, this integrated micro-optical array of structures device directly can be placed the full automatic workstation that has application of sample, hatches, washes plate, scan function to carry out full-automation processes.In addition, also this micro array apparatus can be applied to manual operations.
Embodiment 2
Such as Fig. 3 and shown in Figure 5, in the present embodiment, integrated micro-optical array of structures device by the surface integrated solid substrate 300 and the framework with holes 400 of micro optical structure array form.
The size of described solid substrate 300 can be the Commonly Used Sizes such as 3 inches of 1 inch *, 6 inches of 3 inches, 2 inches * of 0.72 inch *, and its material can be glass or macromolecular material.Described micro optical structure array 310 is integrated in the solid substrate surface, the two one machine-shaping.As shown in Figure 4, the density of micro optical structure array 310 and position can be decided according to demand.Described framework with holes 400 can be the regular sizes such as standard 16 holes, 24 holes, 48 holes, 72 holes or 96 holes, and hole 410 can be the shape such as circular or square, and the hole is through hole, bottomless.
As shown in Figure 7, the outer rim of framework 400 upper surfaces with holes can also be with ledge structure outwardly on surface level 430, so that framework with holes 400 can be placed on as shown in Figure 8 the grillage 500.Preferably, the size of grillage 500 can be measure-alike with standard 96 orifice plates, if framework with holes 400 is 24 holes, then grillage 500 can be placed 4 in conjunction with the framework with holes 400 of solid substrate 300, the advantage of ledge structure 430 is easy to operate, easily is automated operation.
The surface treatment of described solid substrate 300 can itself and framework with holes 400 in conjunction with before or after carry out, by surface treatment, so that the surface of solid substrate 300 has the reactive group that can be combined with biomolecule.If surface treatment is before carried out in solid substrate 300 and framework 400 combinations with holes, then after the surface treatment point sample is finished, make solid substrate 300 and framework 400 combinations with holes, then at the upper surface point bioactive molecule processed of the micro optical structure array 310 that is integrated in solid substrate 300 surfaces.If surface treatment is carried out after solid substrate 300 and framework with holes 400 combinations, then after surface treatment is finished, at the upper surface point bioactive molecule processed of the micro optical structure array 310 that is integrated in solid substrate surface 300.After point sample is finished, again through operations such as fixing, sealing, cleanings, can be directly the integrated micro-optical array of structures device of present embodiment be placed the instrument that has application of sample, hatches, washes the automation functions such as plate, scanning to operate, also can carry out manual operations.
Embodiment 3
Such as Fig. 3 and shown in Figure 9, in the present embodiment, integrated micro-optical array of structures device by the surface integrated the solid substrate 300 of micro optical structure array form with chip fence 600.
The size of described solid substrate 300 can be the Commonly Used Sizes such as 3 inches of 1 inch *, 6 inches of 3 inches, 2 inches * of 0.72 inch *, and its material can be glass or macromolecular material.Described micro optical structure array 310 is integrated in the solid substrate surface, the two one machine-shaping.Described chip fence 600 is elastomeric materials, and quality is soft, is easier to stick on the surface of solid substrate 300, and its function mainly in order to isolate sample, is avoided cross pollution, is fit to the less occasion of testing sample.
First the solid substrate 300 of integrated micro-optical array of structures 310 is carried out surface treatment and make its surface have reactive group, then carry out point sample on the micro optical structure surface.After point sample is finished, through operations such as fixing, sealing, cleanings, afterwards chip fence 600 is pasted on substrate surface again, then namely can with the integrated micro-optical array of structures application of installation of present embodiment in application of sample, hatch, wash the manual operationss such as plate, scanning.Hole 610 sizes of described chip fence 600 and density is flexible choice as required.
(2) structure of micro optical structure
In above-mentioned integrated micro-optical array of structures device, micro optical structure 111 can be to turn photo structure, particularly, can be designed as frustum cone structure, column structure, square column structure, six rod structures or octagon column structure etc.By the described photo structure that turns, can change the direction of dispersing signal, the signal sensor that can be placed in chip body below detects, thereby improves the collection rate of signal.In addition, micro optical structure 111 also can form by turning the set of photo structure and concentration structure, namely the integrated concentration structure in the bottom that turns photo structure, such as convex lens structures and Fresnel Lenses etc.Because turning photo structure mainly relies on reflex that signal is turned to, therefore can turn at micro optical structure reflecting surface (being outside surface) the plating reflectance coating increase signal reflex rate of photo structure, reduce the signal transmission loss, for example metallic reflective coating, the dielectric reflectance coating, inter metal dielectric reflectance coating etc.
When micro optical structure 111 when turning photo structure, the array distribution that is comprised of this kind structure is in the upper surface of solid substrate; When micro optical structure 111 by turning the set of photo structure and concentration structure when forming, this kind structure vertically runs through whole solid substrate from top to bottom from the upper surface of solid substrate, its transfer photo structure is distributed in upper surface of substrate, and concentration structure is positioned at below the substrate.
Embodiment 4
Shown in the sectional side elevation of Figure 10, in the present embodiment, micro optical structure 111 is by turning photo structure and concentration structure forms.Its transfer photo structure is frustum cone structure 111c, and concentration structure is Fresnel Lenses 111d, and it is integrated in and turns the photo structure below.Whole micro optical structure 111 vertically runs through whole solid substrate from top to bottom from the solid substrate surface, wherein frustum cone structure 111c is distributed in the solid substrate surface.The bottom surface of frustum cone structure 111c and the angle of side are designed to a fixed value θ, and this angle θ is between the 45-70 degree, and concrete numerical value is then decided according to the material that it adopts.The round platform interface I of frustum cone structure 111c (namely turning the outside surface of photo structure) is plating reflectance coating surface, total reflection occurs at this interface I place in the light that is distributed in the certain angle scope that the fluorescence molecule layer 111b of round platform upper surface 111a produce, the light that reflects reflects at optically focused interface I I place, then light reflects at optically focused interface I II place, and last light is reflected onto the detector reception that is positioned under the chip body at optically focused interface I V place.
Embodiment 5
Shown in the sectional side elevation of Figure 11, in the present embodiment, micro optical structure 111 is by turning photo structure and concentration structure forms.Its transfer photo structure is frustum cone structure 111c, and its concrete structure design and implementation example 4 is identical, and concentration structure is convex lens 111e, and it is integrated in and turns the photo structure below.Whole micro optical structure 111 vertically runs through whole solid substrate from top to bottom from the solid substrate surface, wherein frustum cone structure 111c is distributed in the solid substrate surface.Total reflection occurs in the light that is distributed in the certain angle scope that the fluorescence molecule layer 111b of round platform upper surface 111a produce at round platform interface I place, and the light that reflects at optically focused interface V the detector that refraction is positioned under the chip body occurs and receives.
It should be noted that, above embodiment is only in order to illustrate technical scheme of the present invention, be not that claim of the present invention is carried out any restriction, those of ordinary skills are under the guidance of the spirit of technical solution of the present invention, the modification that technical solution of the present invention is carried out or be equal to replacement all should be encompassed in the middle of the claim scope of the present invention.
Claims (10)
1. an integrated micro-optical array of structures device is characterized in that, it comprises solid substrate and the framework that is integrated with the micro optical structure array; Described micro optical structure array has many groups, and respectively organizes the micro optical structure array and in one plane be arranged in array; Described framework is combined on the solid substrate and with each group micro optical structure array to be separated.
2. integrated micro-optical array of structures device according to claim 1 is characterized in that,
Described solid substrate by a plurality of structures and size all identical pore structure rearrange equally spacedly; All be integrated with one group of micro optical structure array on the bottom surface of each pore structure inside, and be combined with the reactive group that can be combined with biomolecule on the inside surface of pore structure, outside surface is provided with retaining structure;
Described framework is provided with the hole lattice of a plurality of at grade rectangular array arrangements, the location structure that is provided with at each hole lattice, the accommodating described pore structure of difference in each hole lattice, and the retaining structure phase blocking of location structure and described pore structure outside surface, described solid substrate is fixed on the framework, and the micro optical structure array on each pore structure inner bottom surface is in the same plane.
3. integrated micro-optical array of structures device according to claim 2 is characterized in that, the material of described solid substrate is a kind of in polystyrene, cyclic olefin polymer and the styrene/acrylonitrile copolymer.
4. integrated micro-optical array of structures device according to claim 1 is characterized in that,
Described solid substrate is planar structure, and the described micro optical structure array of respectively organizing is integrated in the upper surface of solid substrate and rectangular array arrangement;
Described framework is incorporated into the upper surface of described solid substrate, which is provided with a plurality of through holes, the rectangular array arrangement of each through hole, and the position of described through hole and described micro optical structure array is corresponding and the micro optical structure array is come out from through hole one by one;
Upper surface in described solid-state base, the position of coming out from described through hole are combined with the reactive group that can be combined with biomolecule.
5. integrated micro-optical array of structures device according to claim 1 is characterized in that,
Described framework is rigid material, with described solid substrate with combine by gluing or mechanical fixing mode; Perhaps
Described framework is elastomeric material, sticks on the upper surface of solid substrate.
6. the described integrated micro-optical array of structures of any one device according to claim 1-5, it is characterized in that, the size of described integrated micro-optical array of structures device is big or small identical with the standard microwell plate, and the described position of respectively organizing the micro optical structure array is corresponding one by one with the hole on the standard microwell plate.
7. integrated micro-optical array of structures device according to claim 1 is characterized in that, described micro optical structure is for turning photo structure, or forms by turning the set of photo structure and concentration structure.
8. integrated micro-optical array of structures device according to claim 7, it is characterized in that, the described photo structure that turns is a kind of in frustum cone structure, column structure, square column structure, six rod structures and the octagon column structure, and the described upper surface that turns photo structure is the plane.
9. integrated micro-optical array of structures device according to claim 8 is characterized in that, the described outside surface that turns photo structure is coated with reflectance coating, and described reflectance coating is a kind of in metallic reflective coating, dielectric reflectance coating and the inter metal dielectric reflectance coating.
10. integrated micro-optical array of structures device according to claim 7 is characterized in that, described concentration structure is a kind of in convex lens and the Fresnel Lenses, and described concentration structure is integrated in the bottom that turns photo structure.
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| CN201210104693XA CN103364344A (en) | 2012-04-10 | 2012-04-10 | Integrated micro-optics structural array device |
| PCT/CN2013/073524 WO2013152679A1 (en) | 2012-04-10 | 2013-03-30 | Integrated micro-optical structure array device |
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| CN107942464A (en) * | 2017-11-22 | 2018-04-20 | 四川云盾光电科技有限公司 | A kind of integral micro-lens array apparatus |
| CN108204971A (en) * | 2016-12-20 | 2018-06-26 | 天津果实科技有限公司 | The device of one-to-one detection is carried out to test paper using color sensor |
| CN110218628A (en) * | 2019-06-19 | 2019-09-10 | 中国科学院半导体研究所 | A kind of digital pcr chip and preparation method thereof |
| WO2020125859A3 (en) * | 2018-12-20 | 2020-09-17 | Leibniz-Institut Für Photonische Technologien E.V. | Arrangement and method for identifying optical properties of a sample, in particular for selective detection of biological molecules and for selecting molecular occupancy |
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