CN103293089A - Reticulocyte analyzer - Google Patents
Reticulocyte analyzer Download PDFInfo
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- CN103293089A CN103293089A CN201310232931XA CN201310232931A CN103293089A CN 103293089 A CN103293089 A CN 103293089A CN 201310232931X A CN201310232931X A CN 201310232931XA CN 201310232931 A CN201310232931 A CN 201310232931A CN 103293089 A CN103293089 A CN 103293089A
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Abstract
The invention discloses a reticulocyte analyzer, comprising a laser shaping lighting unit, a sample processing conveyer unit, a subsequent signal processing unit and a sheath flow pool, wherein the laser shaping lighting unit comprises a light source and a shaping lens structure; laser generated by the light source is shaped by the shaping lens structure; the shaping lens structure comprises at least two shaping lenses which are vertically arranged; at least one cylindrical lens or a double-conical surface lens is arranged inside each of the two shaping lenses; at least one actual or virtual inner focal point exists in the shaping lens structure. At least two vertically arranged shaping lenses are adopted to form the shaping lens structure; the structure modes of the first focal point and the second focal point are thoroughly changed; and the sidelobe phenomenon of a light spot on a short-axis direction is avoided, so that a side-lobe pulse in the subsequent electric signal pulse is thoroughly removed; and the situation of sample misjudgement caused by rusing of sidelobe is also avoided.
Description
Technical field
The present invention relates to flow cytometer, relate in particular to a kind of granulophilocyte analyser.
Background technology
Traditional flow cytometer has been widely used in Arneth's count and granulophilocyte analysis field.Such analyser mainly is made up of lighting unit, sheath flow pool unit, unit, pressure liquid road and signal processing unit.Wherein after the optical alignment shaping that lighting unit sends light source (light source generally adopts the technical approach of laser), becoming xsect is disciform hot spot, exposes to sheath flow pool.Sheath flow pool provides an optical detection zone, in this zone, utilizes sheath stream principle that sample to be tested is taken and is rolled in the sheath stream, makes the sample cell one by one by this zone.After illuminating by this regional illuminated unit of sample cell, send scattered light in full spatial domain, if sample to be tested by fluorescent dyeing, then simultaneously sends fluorescence signal to full spatial domain; Signal processing unit is collected scattered light and fluorescence information according to the difference of emission optic angle degree, and is converted to electric signal.These electric signal can form one dimension histogram or two-dimentional scatter diagram intuitively after screening, handle, analyzing, thereby obtain classification and the count information of leucocyte and granulophilocyte.
Fluorescent dye is DNA and the RNA material sensitive to containing in the granulophilocyte only, and adheres on it.In general, DNA or RNA are more many, and then the fluorescent dye that adheres on it is more many, and the fluorescence signal of collecting in the light path is more strong, distinguish and count granulophilocyte with this.
In the prior art, as shown in Figure 1, the light that the lighting unit of common white blood cell count(WBC) instrument all needs light source 11 is sent is through a central area that is focused on sheath flow pool 2 by 12,13 optical systems of forming.Form an ellipse hot spot on the plane perpendicular to light path.Usually light source is semiconductor laser, and it is angle of divergence difference on orthogonal both direction, is 8 degree such as in one direction the angle of divergence, is on its vertical direction to be 32 degree.
The light beam of this lighting unit output respectively has the focus point of a diverse location on large and small angle, wherein the diverging light of 32 degree focuses on regional center to be measured (hereinafter claiming first focus point), the width that focuses on is about 14 ± 5um, the diverging light of 8 degree is focused on first focus point, the position of 7 ~ 20mm afterwards, it focuses on width should be generally 0.1 ~ 1mm less than the diaphragm that is in the light of design.
Wherein the hot spot of first focus point as shown in Figure 2, uses the ellipse gray area to indicate, and its hot spot is wide to be 14 ± 5um, and length is 60 um ~ 300um, is shaped as ellipse, and short-axis direction flows to consistent with sample to be tested.The hot spot of second focus point also is ellipticity, the wide 0.1 ~ 1mm of hot spot, and length direction does not require, and is general definite according to concrete design proposal.The long axis direction of second focus point flows to consistent, namely orthogonal with the hot spot major axis of first focus point with sample to be tested.
First focus point is the illumination hot spot, and effect is to illuminate by this regional sample to be tested, so that sample sends scattered light or fluorescence.After light beam directly passes sample to be tested, if directly enter photo-translating system, will cause a bigger luminous energy background, thereby make the follow-up signal processing unit a bigger direct current background (energy of direct projection laser is much larger than scattered light or fluorescence) occur, even can cause the saturated of photo-electric conversion element.So, need after zone to be measured, use a bar shaped diaphragm, the laser of direct projection is blocked, for making the bar shaped diaphragm be unlikely to block more useful signal light, so the dimension of this bar shaped diaphragm should be the smaller the better, still, again can not be too small, to such an extent as to can not block laser fully.Therefore, illuminator has designed second focus point after first focus point, and laser is pooled an oval-shaped elongated hot spot, and its spot width is more little, then needs the dimension of the bar shaped diaphragm that blocks more little.Then, less bar shaped diaphragm is arranged on second focus point, reaches the purpose that reduces bar shaped diaphragm dimension.
As mentioned above, first lens in the above-mentioned illuminator are responsible for the light that noise spectra of semiconductor lasers sends and are collected, and carry out shaping to a certain degree, become directional light such as the light that laser instrument is sent.But the light that semiconductor laser sends is angle of divergence difference on orthogonal both direction, and wherein one tunnel divergence half-angle is about 32 degree.This just requires this eyeglass to have one than higher numerical aperture, in general, should require the clear aperature of this eyeglass enough big simultaneously greater than 0.6.
In this case, to general sphere or non-spherical lens, numerical aperture is 0.6, generally all can be near the design limit value of eyeglass.Like this, inevitably some laser has shone the edge of lens.As shown in Figure 3, this a part of light is scattered at first rims of the lens, after follow-up light beam orthopedic systems is handled, can near the illumination hot spot, form the secondary lobe that should not occur, after the sample in zone to be measured passes through this hot spot, the electric pulse image of its formation will be as shown in Figure 4, this other pulse that occurs on the main pulse next door.
Generally speaking, have a lowest threshold at circuit, in order to side-lobe pulse like the shield type, but because sample to be tested is not of uniform size, it is less wherein to have comprised volume ratio, the cells such as blood platelet that the electric pulse that causes simultaneously is smaller, like this, it is too small that this lowest threshold just should not arrange, in case the erroneous judgement of blood platelet signal is garbage signal.Like this, bigger with regard to some volume ratio, the electric pulse that causes simultaneously is bigger cell also, and the side-lobe pulse that its association occurs is mistaken for the cell of small-signal.Thereby the result who causes classifying and count is not accurate enough.
Therefore, prior art awaits further to improve and develop.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of granulophilocyte analyser, on short-axis direction, eliminate the secondary lobe phenomenon fully, make the other pulse in the follow-up electric signal pulse thoroughly eliminate, avoid occurring improving precision of analysis because secondary lobe dashes the situation that causes the sample erroneous judgement.
Technical scheme of the present invention is as follows:
A kind of granulophilocyte analyser, it comprises laser shaping lighting unit, sample process supply unit, follow-up signal processing unit and sheath flow pool, wherein, described laser shaping lighting unit be used for to laser collimate, evenly, converge the sample to be tested that shines described sheath flow pool after shaping is handled; Described sample process supply unit is connected with described sheath flow pool, is used for being taken the sample to be tested of the wrapping up in described sheath flow pool of flowing through by sheath fluid; Described follow-up signal processing unit is connected with described sheath flow pool, for the treatment of the scattered light of described sheath flow pool output;
Described laser shaping lighting unit comprises a light source and a shaping lens structure, the laser that described light source produces sees through described shaping lens structure and carries out shaping, described shaping lens structure comprises at least two shaping lens of vertically arranging, at least there are an interior focus reality or virtual at least in a slice cylindrical lens or double cone face type lens in two shaping lens in the described shaping lens structure.
Described granulophilocyte analyser, wherein, described shaping lens structure comprises two convex cylindrical mirrors, and the bus of two convex cylindrical mirrors is positioned at same straight line, have focus in the reality between two convex cylindrical mirrors, the convex surface of two convex cylindrical mirrors is all towards described light source; Perhaps described shaping lens structure comprises a slice convex cylindrical mirror and a slice shaping concavees lens, has a virtual interior focus between convex cylindrical mirror and the shaping concavees lens, and the concave surface of the convex surface of convex cylindrical mirror and shaping concavees lens is all towards described light source.
Described granulophilocyte analyser, wherein, described shaping lens structure comprises the first convex cylindrical mirror, the second convex cylindrical mirror and the 3rd convex cylindrical mirror, the first convex cylindrical mirror, the photocentre of the second convex cylindrical mirror and the 3rd convex cylindrical mirror is positioned at same straight line, the second convex cylindrical mirror is between the first convex cylindrical mirror and the 3rd convex cylindrical mirror, there is focus in the reality between the second convex cylindrical mirror and the 3rd convex cylindrical mirror, the convex surface of the first convex cylindrical mirror and the 3rd convex cylindrical mirror is all towards described light source, and the convex surface of the described second convex cylindrical mirror is described light source dorsad.
Described granulophilocyte analyser, wherein, described shaping lens structure comprises the 4th convex cylindrical mirror, the 5th convex cylindrical mirror and the 6th shaping concavees lens, the 4th convex cylindrical mirror, the photocentre of the 5th convex cylindrical mirror and the 6th shaping concavees lens is positioned at same straight line, the 5th convex cylindrical mirror is between the 4th convex cylindrical mirror and the 6th shaping concavees lens, there is a virtual interior focus between the 5th convex cylindrical mirror and the 6th shaping concavees lens, the 4th convex cylindrical mirror and convex surface described light source dorsad, the concave surface of the convex surface of described the 5th convex cylindrical mirror and described the 6th shaping concavees lens is all towards described light source.
Described granulophilocyte analyser, wherein, described light source is semiconductor laser, the laser that described semiconductor laser sends in the angle of divergence of Y-direction greater than the angle of divergence at directions X.
Described granulophilocyte analyser, wherein, light after the shaping of described laser shaping lighting unit is ellipse at the hot spot that the zone to be measured of described sheath flow pool forms, its major axis is 60 um ~ 300um, its minor axis is 12 um ~ 18um, and described short-axis direction is consistent with the directions X of the laser that described light source sends.
Described granulophilocyte analyser, wherein, the xsect of described laser shaping lighting unit output light be oval hot spot, light energy distribution is even, the spot center place and apart from the optical energy density difference at spot center 5um place less than 20%.
Described granulophilocyte analyser, wherein, described sample process supply unit comprises air pressure transmission control module and liquid transmission control module, described air pressure transmission control module, liquid transmission control module all are connected with described sheath flow pool;
Described air pressure transmission control module provides the sheath hydraulic coupling of 0.014 ± 0.003MPa, makes sheath fluid stable by described sheath flow pool at a high speed;
Sample after described liquid transmission control module will be handled is delivered to described sheath flow pool, sheath fluid is taken wrapped up in sample by described sheath flow pool;
Described sheath flow pool internal liquid path xsect is circular or square, and its diameter or the length of side are less than 0.3mm.
Described granulophilocyte analyser, wherein, described follow-up signal processing unit comprises light signal processing module, photoelectric conversion module, electric signal processing module, and is connected successively;
Described light signal processing module is collected the scattered light that sample sends, and light splitting is carried out according to the scattering angle of correspondence in the collimation back, converges respectively afterwards, injects photoelectric conversion module;
Described photoelectric conversion module comprises photo-electric conversion elements such as photoelectric cell or photodiode at least, converts the light signal of incident to electric signal;
Described electric signal processing module is extracted peak value or the pulse width information in the above-said current signal, counts, classifies after the examination screening, forms two scatter diagrams or one dimension histogram.
Described granulophilocyte analyser, wherein, described light signal processing module comprises at least one photomultiplier.
A kind of granulophilocyte analyser provided by the invention, adopt at least two shaping lens of vertically arranging to form the shaping lens structure, the angle of divergence that laser instrument is sent is that the light about 8 degree pools first focus point, its spot width is 14 ± 5um, use a beam shaping system that interior focus is arranged, make that light width when first focus point of 32 degree left and right directions is 60 um ~ 300um, and converge to second focal spot, thoroughly change the frame mode of first focus and second focus, avoided the irradiation hot spot secondary lobe phenomenon to occur at short-axis direction, make the other pulse in the follow-up electric signal pulse thoroughly eliminate, avoided occurring dashing the situation that causes the sample erroneous judgement because of secondary lobe, improve precision of analysis, obtained technical very big progress.
Description of drawings
Fig. 1 is the structural representation of granulophilocyte analyser illuminator in the prior art;
Fig. 2 is the structural representation of granulophilocyte analyser illumination hot spot in the prior art;
Fig. 3 is the defective synoptic diagram of granulophilocyte analyser illuminator in the prior art;
Fig. 4 is the side-lobe pulse synoptic diagram of granulophilocyte analyser in the prior art;
Fig. 5 is the structural representation of the laser shaping lighting unit of two convex lens among the present invention;
Fig. 6 is the structural representation of the laser shaping lighting unit of convex lens among the present invention and concavees lens;
Fig. 7 is the structural representation of the laser shaping lighting unit of three convex lens among the present invention;
Fig. 8 is the structural representation of the laser shaping lighting unit of two convex lens among the present invention and concavees lens;
Fig. 9 is the structural representation of granulophilocyte analyser among the present invention.
Embodiment
The invention provides a kind of granulophilocyte analyser, clearer, clear and definite for making purpose of the present invention, technical scheme and effect, below the present invention is described in more detail.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in restriction the present invention.
The invention provides a kind of granulophilocyte analyser, as shown in Figure 9, it comprises laser shaping lighting unit 1, sample process supply unit 3, follow-up signal processing unit 4 and sheath flow pool 5, and described laser shaping lighting unit 1 be used for to laser collimate, evenly, converge the sample to be tested that shines described sheath flow pool 5 after shaping is handled; Described sample process supply unit 3 is connected with described sheath flow pool 5, is used for being taken the sample to be tested of the wrapping up in described sheath flow pool of flowing through by sheath fluid; Described follow-up signal processing unit 4 is connected with described sheath flow pool 5, and the scattered light for the treatment of described sheath flow pool 5 outputs forms two scatter diagrams or one dimension histogram.
Described laser shaping lighting unit 1 comprises a light source 6 and a shaping lens structure, and described light source 6 adopts light source to adopt laser, includes but not limited to semiconductor laser.The laser that described light source 6 produces sees through described shaping lens structure and carries out shaping, as Fig. 5, Fig. 6, Fig. 7 and shown in Figure 8, described shaping lens structure comprises at least two shaping lens of vertically arranging, at least there are an interior focus reality or virtual at least in a slice cylindrical lens or double cone face type lens in two shaping lens in the described shaping lens structure.Thoroughly change the frame mode of first focus and second focus, avoided the irradiation hot spot secondary lobe phenomenon to occur at short-axis direction, improved precision of analysis.
Further obtain, as shown in Figure 5, described shaping lens structure comprises two convex cylindrical mirrors 7, the bus of two convex cylindrical mirrors 7 is positioned at same straight line, there is focus in the reality between two convex cylindrical mirrors 7, the convex surface of two convex cylindrical mirrors 7 is all towards described light source 6, can use to make and the high order aspheric surface lens of beam energy homogenising light is distributed evenly in the zone to be measured of described sheath flow pool 5.Perhaps as shown in Figure 6, described shaping lens structure comprises a slice convex cylindrical mirror 8 and a slice shaping concavees lens 9, have a virtual interior focus between convex cylindrical mirror 8 and the shaping concavees lens 9, the concave surface of the convex surface of convex cylindrical mirror 9 and shaping concavees lens 8 is all towards described light source.
In the most preferred embodiment of the present invention, as shown in Figure 7, described shaping lens structure comprises the first convex cylindrical mirror 10, the second convex cylindrical mirror 15 and the 3rd convex cylindrical mirror 16, the first convex cylindrical mirror 10, the photocentre of the second convex cylindrical mirror 15 and the 3rd convex cylindrical mirror 16 is positioned at same straight line, the second convex cylindrical mirror 15 is between the first convex cylindrical mirror 10 and the 3rd convex cylindrical mirror 16, there is focus in the reality between the second convex cylindrical mirror 15 and the 3rd convex cylindrical mirror 16, the convex surface of the first convex cylindrical mirror 10 and the 3rd convex cylindrical mirror 16 is all towards described light source 6, the convex surface of the described second convex cylindrical mirror 15 is described light source 6 dorsad, the face type curve of all lens minute surfaces can be sphere or aspheric surface, also can use and make the high order aspheric surface lens of beam energy homogenising, make that the energy distribution of system's output facula is non-Gaussian type, thereby the light after the shaping is evenly distributed in the zone to be measured of described sheath flow pool 5.
Further, as shown in Figure 8, described shaping lens structure comprises the 4th convex cylindrical mirror 17, the 5th convex cylindrical mirror 18 and the 6th shaping concavees lens 19, the 4th convex cylindrical mirror 17, the photocentre of the 5th convex cylindrical mirror 18 and the 6th shaping concavees lens 19 is positioned at same straight line, the 5th convex cylindrical mirror 18 is between the 4th convex cylindrical mirror 17 and the 6th shaping concavees lens 19, there is a virtual interior focus between the 5th convex cylindrical mirror 18 and the 6th shaping concavees lens 19, the 4th convex cylindrical mirror 17 and convex surface described light source dorsad, the concave surface of the convex surface of described the 5th convex cylindrical mirror 18 and described the 6th shaping concavees lens 19 is all towards described light source.
In another preferred embodiment of the present invention, described light source is semiconductor laser, the laser that described semiconductor laser sends in the angle of divergence of Y-direction greater than the angle of divergence at directions X.
And the light after the shaping of described laser shaping lighting unit is ellipse at the hot spot that the zone to be measured of described sheath flow pool forms, its major axis is 60 um ~ 300um, its minor axis is 12 um ~ 18um, and described short-axis direction is consistent with the directions X of the laser that described light source sends.
Further, the xsect of described laser shaping lighting unit 1 output light be oval hot spot, and light energy distribution is even, the spot center place and apart from the optical energy density difference at spot center 5um place less than 20%.
In another preferred embodiment of the present invention, described sample process supply unit 3 comprises air pressure transmission control module and liquid transmission control module, and described air pressure transmission control module, liquid transmission control module all are connected with described sheath flow pool 5;
Described air pressure transmission control module provides the sheath hydraulic coupling of 0.014 ± 0.003MPa, makes sheath fluid stable by described sheath flow pool at a high speed;
Sample after described liquid transmission control module will be handled is delivered to described sheath flow pool, sheath fluid is taken wrapped up in sample by described sheath flow pool;
Described sheath flow pool 5 internal liquid path xsects are circular or square, and its diameter or the length of side are less than 0.3mm.
In another preferred embodiment of the present invention, described follow-up signal processing unit 4 comprises light signal processing module, photoelectric conversion module, electric signal processing module, and is connected successively;
Described light signal processing module is collected the scattered light that sample sends, and light splitting is carried out according to the scattering angle of correspondence in the collimation back, converges respectively afterwards, injects photoelectric conversion module;
Described photoelectric conversion module comprises photo-electric conversion elements such as photoelectric cell or photodiode at least, converts the light signal of incident to electric signal;
Described electric signal processing module is extracted peak value or the pulse width information in the above-said current signal, counts, classifies after the examination screening, forms two scatter diagrams or one dimension histogram.And described light signal processing module comprises at least one photomultiplier, and further, described light signal processing module includes but not limited to devices such as photodiode, avalanche photodide, photomultiplier.
Should be understood that application of the present invention is not limited to above-mentioned giving an example, for those of ordinary skills, can be improved according to the above description or conversion that all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (10)
1. granulophilocyte analyser, it comprises laser shaping lighting unit, sample process supply unit, follow-up signal processing unit and sheath flow pool, it is characterized in that, described laser shaping lighting unit be used for to laser collimate, evenly, converge the sample to be tested that shines described sheath flow pool after shaping is handled; Described sample process supply unit is connected with described sheath flow pool, is used for being taken the sample to be tested of the wrapping up in described sheath flow pool of flowing through by sheath fluid; Described follow-up signal processing unit is connected with described sheath flow pool, for the treatment of the scattered light of described sheath flow pool output;
Described laser shaping lighting unit comprises a light source and a shaping lens structure, the laser that described light source produces sees through described shaping lens structure and carries out shaping, described shaping lens structure comprises at least two shaping lens of vertically arranging, at least there are an interior focus reality or virtual at least in a slice cylindrical lens or double cone face type lens in two shaping lens in the described shaping lens structure.
2. want 1 described granulophilocyte analyser according to right, it is characterized in that, described shaping lens structure comprises two convex cylindrical mirrors, the bus of two convex cylindrical mirrors is positioned at same straight line, have focus in the reality between two convex cylindrical mirrors, the convex surface of two convex cylindrical mirrors is all towards described light source; Perhaps described shaping lens structure comprises a slice convex cylindrical mirror and a slice shaping concavees lens, has a virtual interior focus between convex cylindrical mirror and the shaping concavees lens, and the concave surface of the convex surface of convex cylindrical mirror and shaping concavees lens is all towards described light source.
3. granulophilocyte analyser according to claim 1, it is characterized in that, described shaping lens structure comprises the first convex cylindrical mirror, the second convex cylindrical mirror and the 3rd convex cylindrical mirror, the first convex cylindrical mirror, the photocentre of the second convex cylindrical mirror and the 3rd convex cylindrical mirror is positioned at same straight line, the second convex cylindrical mirror is between the first convex cylindrical mirror and the 3rd convex cylindrical mirror, there is focus in the reality between the second convex cylindrical mirror and the 3rd convex cylindrical mirror, the convex surface of the first convex cylindrical mirror and the 3rd convex cylindrical mirror is all towards described light source, and the convex surface of the described second convex cylindrical mirror is described light source dorsad.
4. granulophilocyte analyser according to claim 1, it is characterized in that, described shaping lens structure comprises the 4th convex cylindrical mirror, the 5th convex cylindrical mirror and the 6th shaping concavees lens, the 4th convex cylindrical mirror, the photocentre of the 5th convex cylindrical mirror and the 6th shaping concavees lens is positioned at same straight line, the 5th convex cylindrical mirror is between the 4th convex cylindrical mirror and the 6th shaping concavees lens, there is a virtual interior focus between the 5th convex cylindrical mirror and the 6th shaping concavees lens, the 4th convex cylindrical mirror and convex surface described light source dorsad, the concave surface of the convex surface of described the 5th convex cylindrical mirror and described the 6th shaping concavees lens is all towards described light source.
5. granulophilocyte analyser according to claim 1 is characterized in that, described light source is semiconductor laser, the laser that described semiconductor laser sends in the angle of divergence of Y-direction greater than the angle of divergence at directions X.
6. granulophilocyte analyser according to claim 5, it is characterized in that, light after the shaping of described laser shaping lighting unit is ellipse at the hot spot that the zone to be measured of described sheath flow pool forms, its major axis is 60 um ~ 300um, its minor axis is 12 um ~ 18um, and described short-axis direction is consistent with the directions X of the laser that described light source sends.
7. granulophilocyte analyser according to claim 1, it is characterized in that, the xsect of described laser shaping lighting unit output light be oval hot spot, and light energy distribution is even, the spot center place and apart from the optical energy density difference at spot center 5um place less than 20%.
8. want 1 described granulophilocyte analyser according to right, it is characterized in that, described sample process supply unit comprises air pressure transmission control module and liquid transmission control module, and described air pressure transmission control module, liquid transmission control module all are connected with described sheath flow pool;
Described air pressure transmission control module provides the sheath hydraulic coupling of 0.014 ± 0.003MPa, makes sheath fluid stable by described sheath flow pool at a high speed;
Sample after described liquid transmission control module will be handled is delivered to described sheath flow pool, sheath fluid is taken wrapped up in sample by described sheath flow pool;
Described sheath flow pool internal liquid path xsect is circular or square, and its diameter or the length of side are less than 0.3mm.
9. granulophilocyte analyser according to claim 1 is characterized in that, described follow-up signal processing unit comprises light signal processing module, photoelectric conversion module, electric signal processing module, and is connected successively;
Described light signal processing module is collected the scattered light that sample sends, and light splitting is carried out according to the scattering angle of correspondence in the collimation back, converges respectively afterwards, injects photoelectric conversion module;
Described photoelectric conversion module comprises photo-electric conversion elements such as photoelectric cell or photodiode at least, converts the light signal of incident to electric signal;
Described electric signal processing module is extracted peak value or the pulse width information in the above-said current signal, counts, classifies after the examination screening, forms two scatter diagrams or one dimension histogram.
10. granulophilocyte analyser according to claim 9 is characterized in that, described light signal processing module comprises at least one photomultiplier.
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| CN104949910A (en) * | 2015-05-29 | 2015-09-30 | 广州埃克森生物科技有限公司 | 5-Part differential hematology analyzer optical system |
| CN106547099A (en) * | 2016-11-02 | 2017-03-29 | 北京信息科技大学 | A kind of flow cytometer beam shaping system based on polarized light |
| CN107479204A (en) * | 2017-09-25 | 2017-12-15 | 深圳市皓龙激光设备有限公司 | Laser facula apparatus for shaping and the laser lamp with the laser facula apparatus for shaping |
| CN110530782A (en) * | 2019-09-25 | 2019-12-03 | 迈克医疗电子有限公司 | Eliminate the optical system and method for side-lobe signal interference |
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| JP2008032659A (en) * | 2006-07-31 | 2008-02-14 | Sysmex Corp | Optical system for particle analyzer, and particle analyzer using it |
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| CN104949910A (en) * | 2015-05-29 | 2015-09-30 | 广州埃克森生物科技有限公司 | 5-Part differential hematology analyzer optical system |
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| CN107479204A (en) * | 2017-09-25 | 2017-12-15 | 深圳市皓龙激光设备有限公司 | Laser facula apparatus for shaping and the laser lamp with the laser facula apparatus for shaping |
| CN110530782A (en) * | 2019-09-25 | 2019-12-03 | 迈克医疗电子有限公司 | Eliminate the optical system and method for side-lobe signal interference |
| CN110530782B (en) * | 2019-09-25 | 2024-05-07 | 迈克医疗电子有限公司 | Optical system and method for eliminating side lobe signal interference |
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Application publication date: 20130911 |