CN103285946A - Biochip and control method thereof - Google Patents
Biochip and control method thereof Download PDFInfo
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- CN103285946A CN103285946A CN2013102036569A CN201310203656A CN103285946A CN 103285946 A CN103285946 A CN 103285946A CN 2013102036569 A CN2013102036569 A CN 2013102036569A CN 201310203656 A CN201310203656 A CN 201310203656A CN 103285946 A CN103285946 A CN 103285946A
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Abstract
The invention provides a novel biochip and a control method of the novel biochip, and in particular relates to a biochip of an integrated electroosmotic pump. The biochip is formed by a sample cell, a micro channel, a micro reaction cavity body and a micro electroosmotic pump, wherein by adjusting the negative pressure generated by the micro electroosmotic pump, one of the following contents or a combination thereof is controlled: sample introduction, reaction and cleaning of a sample. According to the biochip and the control method of the biochip, the equipment is simple, the operation is simple and convenient, and automation and integration of the biochip are realized easily.
Description
Technical field
The present invention relates to the biochip applications technology, a kind of biochip control method based on electroosmotic pump is provided especially.
Background technology
In the prior art, micro-fluidic chip is an emerging technology platform.On more than one square centimeters chip, by the microchannel control fluid of networking, finish the various operations of conventional chemical or biology laboratory.
At present, much the biochip based on microflow control technique is developed, and purpose is to reduce the integrated and full-automatic of the consumption of sample, the reaction uniformity that improves biological sample and reaction efficiency, reinforcement biochip system.In the use of biochip, sample mostly is fluid, the body fluid of humans and animals for example, and therefore, controlling of fluid is the core content that biochip is controlled.At present, be applied to the fluid operated of biochip and realized based on external pump valve system that mainly contrast can be integrated in the Micropump unit of chip internal, such control mode obviously is difficult to satisfy low-cost and highly integrated demand.It is a kind of fluid type of drive very important in the micro-fluidic chip that EOF drives, but be that the charge characteristic on chip channel surface is realized owing to the EOF that generally adopts at present drives what rely on, therefore, sample solution is to be under the high electric field environment, under such condition, the phenomenon that electric charge absorption, electrophoretic migration etc. are unfavorable for reacting and subsequent detection is analyzed takes place in component in the biological sample easily, press for a kind of being integrated on the biochip, can realize that again sample solution does not have the fluid driving method of electric field environment simultaneously.
Summary of the invention
Controlling the weak point of technology at above-mentioned biochip, the invention provides a kind of novel biochip and control method thereof, or rather, is a kind of biochip of integrated electroosmotic pump.This biochip is made of sample cell, microchannel, little reaction cavity and little electroosmotic pump, controls one of following content or its combination by the negative pressure of regulating little electroosmotic pump generation: the sample introduction of sample, reaction or cleaning.
According to a kind of biochip and the control method thereof that provide, described biochip is made of trilaminate material, and wherein bottom and quilting material can be silica, glass, quartz, Merlon, polymethyl methacrylate, dimethyl silicone polymer etc.; The intermediate layer is the double faced adhesive tape with penetrating micro-structural.
According to a kind of biochip and the control method thereof that provide, described biochip carries out processing and preparing by existing micro-processing technology, and wherein three-decker is to rely on the adhibit quality in intermediate layer and realize bonding.
According to a kind of biochip and the control method thereof that provide, the bottom of described little reaction cavity is that bioprobe is fixedly arranged on the substrate material surface, specifically comprises nucleic acid probe, protein, antigen-antibody, enzyme and substrate; Above-mentioned substance can carry out immobilization by the mode of chemical crosslinking or physical absorption.
According to a kind of biochip and the control method thereof that provide, described little electroosmotic pump is connected in the downstream of microchannel and little reaction cavity.
According to a kind of biochip and the control method thereof that provide, described little electroosmotic pump is made of filling pillar passage and the buffering area that crosses of two Junction on bend aheads.Described filling pillar channel interior is full of filler.Filler is selected a kind of in silica gel, ion exchange resin, polymer microsphere, inorganic sphere or amorphous filler, the interior moulding filler for use, and particle diameter is 50～5000 nanometers, and the filler in the packed column of two Junction on bend aheads is respectively with two kinds of opposite electric charges.
According to a kind of biochip and the control method thereof that provide, the buffering area that crosses of described electroosmotic pump can be connected with the fluid passage on the biology, and the sample solution that is used for chip is controlled.
According to a kind of biochip and the control method thereof that provide, the sample introduction of described sample, reaction and cleaning are to be driving force with the negative pressure that electroosmotic pump provides, and realize in conjunction with the channel geometries on the chip.
According to a kind of biochip and the control method thereof that provide, described sample is the fluid of solution state, can be human or animal's body fluid, comprises whole blood, serum, urine, saliva, also can be the aqueous solution.
According to a kind of biochip and the control method thereof that provide, in the described biochip fluid operated a kind of do not have under the electric field environment realize.
According to a kind of biochip and the control method thereof that provide, the control method equipment of described biochip is simple, easy and simple to handle, the automation of very easy realization biochip and integrated.
Description of drawings
Fig. 1. a kind of biochip structural representation.Wherein, a is sample cell, and b is the microchannel, and c is little reaction cavity, and d is electroosmotic pump, and e is fixing bioprobe.
Fig. 2. a kind of cross section structure schematic diagram of biochip.Wherein, f is the cover plate layer, and g is the intermediate layer, and h is basalis, and i is microchannel or little reaction cavity, and j is bioprobe.
Fig. 3. used electroosmotic pump structural representation among the present invention.Wherein, k is the buffering area that crosses, and 1 is negative electricity packed column (connecting the external electric field negative pole), and m is positive electricity packed column (connecting the external electric field positive pole).
Specific embodiments
The following examples will give further instruction to the present invention in conjunction with Figure of description.
A kind of planar structure with biochip as shown in Figure 1, biochip is made of sample cell a, microchannel b, unreacted cavity c, electroosmotic pump d, e is fixing bioprobe.Further, its cross-sectional structure as shown in Figure 2, chip is three-decker, comprises cover plate layer f, intermediate layer g and basalis h, bioprobe j is fixed in the bottom of microchannel or little reaction cavity i.
Be integrated on the biochip the electroosmotic pump structure as shown in Figure 3, this electroosmotic pump is positioned at the downstream of little reaction cavity and microchannel.The packed column passage 1 and the m that mainly contain cross buffering area k and two Junction on bend aheads fill the glass microsphere of particle diameter 50～5000nm respectively in the two packed column passages, the surface is modified positive charge and negative electrical charge respectively.
During experiment, at first the chip internal passage all is full of the 0.1M borate buffer solution, the packed column 1 of electroosmotic pump connects the negative pole of extra electric field, and packed column m connects the positive pole of extra electric field, with this understanding, the flow direction of electroosmotic pump is the two ends of buffering area trend Junction on bend ahead passage of crossing.Therefore, when electroosmotic pump was worked, the fluid among the b in the biochip, c, the k all moved towards electroosmotic pump.The speed of operation is regulated and is controlled by electric-field intensity.
During sample introduction, sample solution flows to the microchannel from sample cell under the effect of electroosmotic pump; During reaction, sample solution flows to little reaction cavity, carries out the biological or chemical reaction with the reagent that is fixed in reaction cavity inside; After reaction is finished, continue in sample cell, to add cleaning fluid, then can realize the cleaning to reaction cavity.
Adopt micro-fluidic chip provided by the invention and control method thereof since in passage integrated filling pillar electroosmotic pump, the controlling in the process of sample, biological sample is in no electric field environment simultaneously.With this understanding, not only can accurately regulate and control sample introduction, reaction and the cleaning of sample, and the operating condition gentleness, can not have a negative impact to sample.
Claims (9)
1. a biochip and control method thereof, it is characterized in that, this biochip is made of sample cell, microchannel, little reaction cavity and little electroosmotic pump, controls one of following content or its combination by the negative pressure of regulating little electroosmotic pump generation: the sample introduction of sample, reaction or cleaning.
2. according to the described a kind of biochip of claim 1 and control method thereof, it is characterized in that the material of described biochip is by one or more the combination in silica, glass, quartz, Merlon, polymethyl methacrylate, the dimethyl silicone polymer etc.
3. according to the described a kind of biochip of claim 1 and control method thereof, it is characterized in that, the bottom of described little reaction cavity is that biology, the required reagent of chemical reaction are fixedly arranged on the substrate material surface, specifically comprises nucleic acid probe, protein, antigen-antibody, enzyme and substrate; Above-mentioned substance can carry out immobilization by the mode of chemical crosslinking or physical absorption.
4. according to the described a kind of biochip of claim 1 and control method thereof, it is characterized in that described little electroosmotic pump is connected in the downstream of microchannel and little reaction cavity.
5. according to the described a kind of biochip of claim 1 and control method thereof, it is characterized in that described little electroosmotic pump is made of filling pillar passage and the buffering area that crosses of two Junction on bend aheads.
6. according to claim 1 and 5 described a kind of biochip and control methods thereof, it is characterized in that, described filling pillar channel interior is full of the filler that particle diameter is 50～5000 nanometers, comprises silica gel, ion exchange resin, polymer microsphere, inorganic sphere or amorphous filler, interior moulding filler etc.
7. according to claim 1 and 6 described a kind of biochip and control methods thereof, it is characterized in that the filler in the packed column of described two Junction on bend aheads is respectively with two kinds of opposite electric charges.
8. according to claim 1 and 6 described a kind of biochip and control methods thereof, it is characterized in that the described buffering area that crosses can be connected with the fluid passage on the biology, the sample solution that is used for chip is controlled.
9. according to the described a kind of biochip of claim 1 and control method thereof, it is characterized in that, the sample introduction of described sample, reaction and cleaning are that the negative pressure that provides with electroosmotic pump is driving force, in the biochip fluid operated a kind of do not have under the electric field environment realize.
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| CN2013102036569A CN103285946A (en) | 2013-05-27 | 2013-05-27 | Biochip and control method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105772117A (en) * | 2014-12-22 | 2016-07-20 | 卡梅德生物科技（天津）有限公司 | Experimental chip used for detection of chemical components in biological laboratory |
| CN110479391A (en) * | 2019-08-05 | 2019-11-22 | 湖北医药学院 | A kind of low-voltage high-performance electric osmose Micropump chip based on solid-state track etching nano-pore |
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| CN1419954A (en) * | 2001-11-15 | 2003-05-28 | 中国科学院大连化学物理研究所 | Chip micro flow electroosmosis pump |
| US20070264320A1 (en) * | 2006-05-09 | 2007-11-15 | The Regents Of The University Of California | Microfluidic device for forming monodisperse lipoplexes |
| CN102059162A (en) * | 2002-06-28 | 2011-05-18 | 哈佛学院董事会 | Microfluidic device |
| CN102586226A (en) * | 2011-01-14 | 2012-07-18 | 中国科学院大连化学物理研究所 | Microfluidic chip nucleic acid purification platform based on continuous liquid drop control |
| WO2012109138A1 (en) * | 2011-02-07 | 2012-08-16 | President And Fellows Of Harvard College | Systems and methods for splitting droplets |
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- 2013-05-27 CN CN2013102036569A patent/CN103285946A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1419954A (en) * | 2001-11-15 | 2003-05-28 | 中国科学院大连化学物理研究所 | Chip micro flow electroosmosis pump |
| CN102059162A (en) * | 2002-06-28 | 2011-05-18 | 哈佛学院董事会 | Microfluidic device |
| US20070264320A1 (en) * | 2006-05-09 | 2007-11-15 | The Regents Of The University Of California | Microfluidic device for forming monodisperse lipoplexes |
| CN102586226A (en) * | 2011-01-14 | 2012-07-18 | 中国科学院大连化学物理研究所 | Microfluidic chip nucleic acid purification platform based on continuous liquid drop control |
| WO2012109138A1 (en) * | 2011-02-07 | 2012-08-16 | President And Fellows Of Harvard College | Systems and methods for splitting droplets |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105772117A (en) * | 2014-12-22 | 2016-07-20 | 卡梅德生物科技（天津）有限公司 | Experimental chip used for detection of chemical components in biological laboratory |
| CN110479391A (en) * | 2019-08-05 | 2019-11-22 | 湖北医药学院 | A kind of low-voltage high-performance electric osmose Micropump chip based on solid-state track etching nano-pore |
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