CN103272657A - Liquid storage method of micro-fluidic chip - Google Patents
Liquid storage method of micro-fluidic chip Download PDFInfo
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- CN103272657A CN103272657A CN201310203220XA CN201310203220A CN103272657A CN 103272657 A CN103272657 A CN 103272657A CN 201310203220X A CN201310203220X A CN 201310203220XA CN 201310203220 A CN201310203220 A CN 201310203220A CN 103272657 A CN103272657 A CN 103272657A
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Abstract
The invention provides a liquid storage method of a micro-fluidic chip; in the micro-fluidic chip, a liquid regent is pre-stored in a specific position in the chip in a sealed liquid capsule manner; and the using principle of the liquid storage method is as follows: with the help of external force, a liquid capsule is damaged partly or extensively, and therefore, the liquid reagent stored inside is released into the specific position in the micro-fluidic chip. By using the liquid storage method disclosed by the invention, liquid reagents in certain volumes and types can be pre-fixed, stored and sealed in the micro-fluidic chip; the liquid reagents can be controlled to release very easily through a simple mechanical method; and the liquid storage method disclosed by the invention plays an extremely important role and has great practical significance in application of micro-fluidic chips.
Description
Technical field
The present invention relates to the micro-fluidic chip technical field, particularly relate to a kind of liquid storage method of micro-fluidic chip.
Background technology
Micro-fluidic chip technology (Microfluidics) is that basic operation units such as the sample preparation of biological, chemistry, medical analysis process, reaction, separation, detection are integrated on the chip of a micro-meter scale, finishes the analysis overall process automatically.Because it in the great potential in fields such as biology, chemistry, medical science, has developed into the brand-new research field of subject crossing such as a biology, chemistry, medical science, fluid, electronics, material, machinery.
Microfluid is controlled the core content that technology is the micro-fluidic chip technology, also is extremely important application foundation in the micro-fluidic chip.Fluid operated technology on the micro-fluidic chip comprises storage, driving, traffic direction, flow velocity regulation and control of fluid etc.In above-mentioned several fluid operated technology, several technology of back have obtained deep research and have used widely, and liquid is in the storage of micro-fluidic chip inside, and relevant research is considerably less.This be because, at present used micro-fluidic chip mostly is disposable use, and common practice is again liquid reagent or sample to be introduced micro-fluidic chip inside in use.But, if can develop a kind of ad-hoc location that liquid reagent can be stored in advance in the micro-fluidic chip, and the method that can the liquid of storage inside be discharged at special time is for realizing the automation of micro-fluidic chip in biological, chemical reaction and detection thereof, integrated, significant.
Summary of the invention
The present invention proposes a kind of liquid storage method of micro-fluidic chip, and in this micro-fluidic chip, liquid reagent is that the liquid scrotiform formula that seals is stored in ad-hoc location in the chip in advance.
The invention provides a kind of liquid storage method of micro-fluidic chip, it is characterized in that, what the material of described micro-fluidic chip can be among glass, silicon, quartz, Merlon, polymethyl methacrylate, the dimethyl silicone polymer etc. is a kind of, or its combination.
The invention provides a kind of liquid storage method of micro-fluidic chip, it is characterized in that, described liquid comprises aqueous phase solution, also comprises organic phase solution.
The invention provides a kind of liquid storage method of micro-fluidic chip, it is characterized in that, described liquid capsule material can be glass, Merlon, polymethyl methacrylate, dimethyl silicone polymer, polytetrafluoroethylene (PTFE) etc.
The invention provides a kind of liquid storage method of micro-fluidic chip, it is characterized in that, described liquid capsule can be cylinder type, sphere or rectangular build, storage inside solution, and the outside is full-closed structure.
The invention provides a kind of liquid storage method of micro-fluidic chip, it is characterized in that, the use principle of described micro-fluidic chip is, effect makes liquid capsule part or large tracts of land breakage by external force, thereby the liquid reagent of storage inside is released into ad-hoc location in the micro-fluidic chip.
The invention provides a kind of liquid storage method of micro-fluidic chip, it is characterized in that, described external force refers to that the outside puts on the mechanicals efforts of liquid capsule, comprises extruding, knocks, acupuncture, vibrations etc.
The liquid storage method of micro-fluidic chip provided by the present invention is to place the ad-hoc location of micro-fluidic chip to realize by the sealing fluid capsule with the outside preparation.Simple in structure, simple operation have application prospect extremely widely in fields such as chemistry, biology, medical science.
Description of drawings
Fig. 1. the structural representation of the liquid storage part of a kind of micro-fluidic chip provided by the invention.Wherein, A is the passage of micro-fluidic chip; B is the liquid storage cavity; C is liquid; D is the liquid capsule.
Fig. 2. the micro-fluidic liquid capsule structural section schematic diagram in the embodiment of the invention one, wherein A ' is micro-fluidic chip, and B ' is the liquid capsule, and C ' is for being stored in the liquid reagent in the liquid capsule.
Fig. 3. the micro-fluidic liquid capsule structural section schematic diagram in the embodiment of the invention two, wherein a is micro-fluidic chip, and b is the liquid of liquid capsule 1 and storage thereof, and c is the liquid reagent that is sealed in the liquid capsule 2 in the liquid capsule 1 and stores.
Fig. 4. the micro-fluidic liquid capsule structural section schematic diagram in the embodiment of the invention three, wherein d is micro-fluidic chip, and e is liquid capsule 1, and f is liquid capsule 2.
Specific embodiments
The following examples will give further instruction to the present invention in conjunction with Figure of description.
Fig. 1 is the structural representation of the liquid storage part of a kind of micro-fluidic chip provided by the invention, liquid capsule D in this micro-fluidic chip is sealed among the liquid storage cavity B, because liquid capsule size greater than the passage A of micro-fluidic chip, can be limited in the liquid storage cavity by the microchannel when therefore preserving.During use, rely on external force, make the breakage of liquid capsule, thereby the liquid reagent C that is stored in liquid capsule inside is released then, enters the microchannel.
The micro-fluidic liquid storage chip of 1 one kinds of single liquid capsules of embodiment
A kind of micro-fluidic liquid storage chip that present embodiment provides, structure as shown in Figure 2, liquid reagent C ' is stored and is sealed in the glass metal capsule B ', whole liquid capsule then is restricted to micro-fluidic chip A ' inside.During use, adopt and knock mode, it is broken that the glass metal capsule is taken place, thereby make liquid reagent C ' be discharged in the micro-fluidic chip.
Present embodiment provides a kind of micro-fluidic memory cell of single liquid capsule, and by liquid storage unit like the different parts design class in micro-fluidic chip, the storage and the location that then can be implemented in different liquids reagent in the micro-fluidic chip discharge.
The micro-fluidic liquid storage chip of bag type biliquid capsule in 2 one kinds of the embodiment
The micro-fluidic liquid storage chip of a kind of double reagent that present embodiment provides, structure as shown in Figure 3, the liquid capsule c that comprises liquid reagent 2 is stored and is sealed in liquid capsule b and the reagent 1, whole liquid capsule b then is restricted to micro-fluidic chip a inside.During use, adopt and knock mode, it is broken that liquid capsule b and c are taken place, thereby make mixing material reagent be discharged in the micro-fluidic chip.
Present embodiment provides a kind of micro-fluidic memory cell that contains type biliquid capsule, by liquid storage unit like the privileged site design class in micro-fluidic chip, then can be implemented in mixing and the release of different liquids reagent in the micro-fluidic chip.
The micro-fluidic liquid storage chip of 3 one kinds of parallel type biliquids of embodiment capsule
The micro-fluidic liquid storage chip of a kind of double reagent that present embodiment provides, structure with different among the embodiment 2 be as shown in Figure 4, and the liquid capsule f that comprises the liquid capsule e of liquid reagent 1 and comprise liquid reagent 2 is restricted to micro-fluidic chip d inside simultaneously.During use, adopt and knock mode, can make liquid capsule b and c successively or fragmentation takes place simultaneously, thereby make liquid reagent be discharged in the micro-fluidic chip successively or simultaneously.
Present embodiment provides a kind of micro-fluidic memory cell of parallel type biliquid capsule, by liquid storage unit like the privileged site design class in micro-fluidic chip, then can be implemented in release and the mixing of different liquids reagent in the micro-fluidic chip.
Use liquid storage method provided by the invention, can be at the liquid reagent of the inner predetermined fixed of micro-fluidic chip, storage and sealing certain volume and type, by simple mechanical means, the release of handling liquids reagent at an easy rate.This liquid storage method has important role and practical significance in the application of micro-fluidic chip.
Claims (8)
1. the liquid storage method of micro-fluidic chip is characterized in that, in this micro-fluidic chip, liquid reagent is that the liquid scrotiform formula that seals is stored in ad-hoc location in the chip.
2. according to the liquid storage method of the described micro-fluidic chip of claim 1, it is characterized in that, the material of described micro-fluidic chip can be among glass, silicon, quartz, Merlon, polymethyl methacrylate, the dimethyl silicone polymer etc. a kind of, or its combination.
3. according to the liquid storage method of the described micro-fluidic chip of claim 1, it is characterized in that described liquid comprises aqueous phase solution, also comprise organic phase solution.
4. according to the liquid storage method of the described micro-fluidic chip of claim 1, it is characterized in that described liquid capsule material can be glass, Merlon, polymethyl methacrylate, dimethyl silicone polymer, polytetrafluoroethylene (PTFE) etc.
5. according to the liquid storage method of the described micro-fluidic chip of claim 1, it is characterized in that described liquid capsule can be cylinder type, sphere or rectangular build, storage inside solution, the outside is full-closed structure.
6. according to the liquid storage method of the described micro-fluidic chip of claim 1, it is characterized in that, the use principle of described micro-fluidic chip is, effect makes liquid capsule part or large tracts of land breakage by external force, thereby the liquid reagent of storage inside is released into ad-hoc location in the micro-fluidic chip.
7. according to the liquid storage method of claim 1 and 6 described micro-fluidic chips, it is characterized in that described external force refers to that the outside puts on the mechanicals efforts of liquid capsule, comprise extruding, knock, acupuncture, vibrations etc.
8. according to the liquid storage method of the described micro-fluidic chip of claim 1, it is characterized in that the liquid storage method of described micro-fluidic chip is to place the ad-hoc location of micro-fluidic chip to realize by the sealing fluid capsule with the outside preparation.Simple in structure, simple operation have application prospect extremely widely in fields such as chemistry, biology, medical science.
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| CN201310203220XA CN103272657A (en) | 2013-05-27 | 2013-05-27 | Liquid storage method of micro-fluidic chip |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104399540A (en) * | 2014-12-10 | 2015-03-11 | 天津微纳芯科技有限公司 | Centrifugal effect-based micro-fluidic chip liquid reagent releasing method |
| CN106140334A (en) * | 2015-03-25 | 2016-11-23 | 北京化工大学 | Micro-fluidic chip detection reaction unit with reagent storage function |
| CN106198848A (en) * | 2016-08-17 | 2016-12-07 | 山东省科学院海洋仪器仪表研究所 | Extend the device and method of situ wet chemistry online analytical instrument reagent holding time |
| CN106413896A (en) * | 2014-04-10 | 2017-02-15 | 10X基因组学有限公司 | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
| CN106434282A (en) * | 2016-10-27 | 2017-02-22 | 杭州安杰思生物科技有限公司 | Micro-fluidic vacuole extrusion mechanism |
| CN108295912A (en) * | 2018-01-12 | 2018-07-20 | 天津微纳芯科技有限公司 | The pre-packaged device and its open method of micro-fluidic chip reagent |
| CN108931639A (en) * | 2017-05-25 | 2018-12-04 | 浙江清华长三角研究院萧山生物工程中心 | The chip and reagent of cortisol detection in a kind of saliva |
| CN109383034A (en) * | 2014-05-05 | 2019-02-26 | 康宁股份有限公司 | It is used to form and the method for dismantling airtight sealing chamber |
| CN109959761A (en) * | 2019-04-11 | 2019-07-02 | 石家庄禾柏生物技术股份有限公司 | It is a kind of for discharging the device of fluid |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106413896A (en) * | 2014-04-10 | 2017-02-15 | 10X基因组学有限公司 | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
| CN106413896B (en) * | 2014-04-10 | 2019-07-05 | 10X基因组学有限公司 | For encapsulating and dividing fluid means, system and method and its application of reagent |
| CN109383034A (en) * | 2014-05-05 | 2019-02-26 | 康宁股份有限公司 | It is used to form and the method for dismantling airtight sealing chamber |
| CN109383034B (en) * | 2014-05-05 | 2021-08-10 | 康宁股份有限公司 | Method for forming and for disassembling hermetically sealed chamber |
| CN104399540A (en) * | 2014-12-10 | 2015-03-11 | 天津微纳芯科技有限公司 | Centrifugal effect-based micro-fluidic chip liquid reagent releasing method |
| CN106140334B (en) * | 2015-03-25 | 2017-11-21 | 北京化工大学 | Micro-fluidic chip detection reaction unit with reagent store function |
| CN106140334A (en) * | 2015-03-25 | 2016-11-23 | 北京化工大学 | Micro-fluidic chip detection reaction unit with reagent storage function |
| CN106198848A (en) * | 2016-08-17 | 2016-12-07 | 山东省科学院海洋仪器仪表研究所 | Extend the device and method of situ wet chemistry online analytical instrument reagent holding time |
| CN106198848B (en) * | 2016-08-17 | 2018-05-29 | 山东省科学院海洋仪器仪表研究所 | Extend the device and method of live wet-chemical online analytical instrument reagent holding time |
| CN106434282A (en) * | 2016-10-27 | 2017-02-22 | 杭州安杰思生物科技有限公司 | Micro-fluidic vacuole extrusion mechanism |
| CN108931639A (en) * | 2017-05-25 | 2018-12-04 | 浙江清华长三角研究院萧山生物工程中心 | The chip and reagent of cortisol detection in a kind of saliva |
| CN108295912A (en) * | 2018-01-12 | 2018-07-20 | 天津微纳芯科技有限公司 | The pre-packaged device and its open method of micro-fluidic chip reagent |
| CN108295912B (en) * | 2018-01-12 | 2020-06-30 | 天津微纳芯科技有限公司 | Pre-packaging device for microfluidic chip reagent and opening method thereof |
| CN109959761A (en) * | 2019-04-11 | 2019-07-02 | 石家庄禾柏生物技术股份有限公司 | It is a kind of for discharging the device of fluid |
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Application publication date: 20130904 |