CN103272479B - The sorption and desorption device of acid active Chinese drug component protein ingredient film and desorption method - Google Patents
The sorption and desorption device of acid active Chinese drug component protein ingredient film and desorption method Download PDFInfo
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Abstract
The sorption and desorption device of acid active Chinese drug component proteinaceous components film and desorption method.This device is made up of the double-deck stock chest of membrane module, circulating pump, extract or stripping liquid, penetrating fluid stock chest, Pressure gauge, flowmeter, water bath with thermostatic control, peristaltic pump, stainless steel pipes.The method is: constant temperature under 0.25MPa, and crossflow velocity is 2m/s, and ultrafiltration absorption content is the acid active Chinese drug component protide extract solution 24h of 98%, calculated activity ingredient adsorption amount.Film after absorption is contained on desorption apparatus.By strippant, constant temperature, is less than 0.05MPa and crossflow velocity carries out desorb under 0.1-1m/s condition at pressure, gets stripping liquid sample, obtain desorption efficiency 90%-99%.The method and device are applicable to the film subtractive process of acid Chinese medicine proteinaceous components, especially removing pyrogen from injections and resin process, greatly can reduce the loss late of this active component, improve its rate of transform, and film obtains regeneration preferably simultaneously.
Description
One. technical field
The present invention relates to a kind of sorption and desorption device and desorption method of acid active Chinese drug component proteinaceous components film.The method is applicable to the Chinese medicinal film subtractive process that active component is proteinaceous components, especially in traditional Chinese medicine depyrogenation and resin process, greatly can reduce the loss late of protide active component, improve its rate of transform, and film obtains regeneration preferably simultaneously.The invention belongs to the Extraction and separation technical field of Chinese medicine preparation.
Two. background technology
There is in Chinese medicine system relative to traditional isolation and purification method application of membrane separation technology the advantage of its uniqueness: separation process is low without phase transformation, normal-temperature operation, energy consumption, not with an organic solvent, the production cycle can be shortened, reduce loss of effective components, be beneficial to environmental protection and safety in production; Select suitable membrane material can obtain higher separation selectivity, keep the corresponding traits of former side thus the curative effect of guarantee finished product; Good impurity removing effect, can remove bacterium and pyrogen while of extending the Chinese medicine storage time; Be specially adapted to the separation of heat-sensitive ingredients in Chinese medicine, concentrate; Can realize serialization, automation mechanized operation, technological parameter is stablized controlled, and easy and other PROCESS COUPLING, meet the requirement of Chinese medicine production modernization.Since the eighties, UF membrane starts the production being applied to Chinese medicine preparation.Receive much attention.Wherein maximum with the application of milipore filter, relate generally to the following aspects: the extraction of (1) Effective Component of Chinese Medicine.(2) production of oral liquid.(3) preparation of medicinal extract preparation.(4) removal of thermal source.(5) from pharmacy waste water, effective ingredient is reclaimed.
Especially employing membrane technology replaces the pyrogen in traditional absorption and heating removal parenteral solution, effectively can solve the medicine sex change that conventional method is brought, generate new sediment, the problems such as flow process is complicated, this all has very large meaning to the production of traditional Chinese medicine injection and new product development.In addition, be separated if having employed resin adsorption in technique early stage, after also can remain certain resin wear thing in parenteral solution.When removing during employing membrane separation technique in both cases, separation membrane surface often adsorbs certain active ingredient of Chinese herbs makes its rate of transform low, and waste natural resources of Chinese medicinal materials, increases production cost.Therefore need to find suitable method to solve this problem produced in film application process.Do not find that pertinent literature is reported at present.
Three. summary of the invention
, problem of resource waste low for the rate of transform occurred in the pyrogen adopted in membrane technology removal parenteral solution and resin process, technical problem to be solved by this invention is just to provide a kind of sorption and desorption device and desorption method of acid active Chinese drug component proteinaceous components film.The method is applied in ultrafiltrationmembrane process depyrogenation and resin process, greatly can reduce the loss late of acid active Chinese drug component proteinaceous components, improve its rate of transform, and film obtains regeneration preferably simultaneously.
the object of the invention is to be realized by following technical scheme:
A kind of sorption and desorption device of acid active Chinese drug component proteinaceous components film, it is characterized in that this device is by membrane module 1, circulating pump 2, double-deck stock chest 3, penetrating fluid stock chest, 4, Pressure gauge 5, flowmeter 6, water bath with thermostatic control 7, peristaltic pump 8, stainless steel pipes 9 is made, wherein double-deck stock chest 3 is connected with circulating pump 2 by stainless steel pipes 9, be connected with Pressure gauge 5 by stainless steel pipes 9 again, be connected with membrane module 1 by stainless steel pipes 9 again, again by stainless steel pipes 9 and Pressure gauge 5, again by stainless steel pipes 9 and flowmeter 6, be connected with double-deck stock chest 3 by stainless steel pipes 9 again, a penetrating fluid conveying stainless steel pipes 9 is had in membrane module 1 lower end, penetrating fluid stock chest 4 is placed below stainless steel pipes 9, water bath with thermostatic control 7 is connected with peristaltic pump 8 by silica gel hose 12, be connected by the chuck import 10 of silica gel hose 12 with double-deck stock chest 3 again, the JO 11 of double-deck stock chest 3 is connected with water bath with thermostatic control 7 by silica gel hose 12.
The sorption and desorption device of described acid active Chinese drug component proteinaceous components film, it is characterized in that described membrane module 1 is divided into organic plate film assembly, ceramic film component or metal film assembly, wherein organic plate film assembly is clipped between upper steel plate 15 and lower steel plate 18 by organic film 13, wherein upper steel plate 15 is inverted plate-like, the Pan Bi of axis has two holes, the short steel pipes with screw thread mouth is welded respectively in the position in hole, for import 16 and outlet 17, be connected with stainless steel pipes 9 respectively, the surface design of lower steel plate 18 has 6-12 road cast groove, per pass cast groove is all designed with a permeate flow and portals, all stainless steel pipes 9 is carried to be connected with the penetrating fluid bottom lower steel plate 18, upper and lower two steel plates add tightening seal by a circular iron hoop 19, ceramic film component or metal film assembly be by tubular ceramic film or tubular metal film 14 by upper-lower seal circle vertical seal in stainless steel enrichment case, stainless steel enrichment case upper and lower side is equipped with screw thread mouth, be connected with stainless steel pipes 9, there is a penetrating fluid delivery outlet lower end, side of stainless steel enrichment case, penetrating fluid, by stainless steel pipes 9, flows in penetrating fluid stock chest 4.
The sorption and desorption device of described acid active Chinese drug component proteinaceous components film, is characterized in that described organic film is poly (ether sulfone) film, PS membrane; Ceramic membrane is silicon oxide film, oxidation titanium film; Metal film is SUS316 film, SLS316L film.
The sorption and desorption device of described acid active Chinese drug component proteinaceous components film carries out the method for sorption and desorption, it is characterized in that sorption and desorption method step is as follows:
A. the extract solution 10L of compound concentration to be the content of 1-5g/L the be acidic protein active component of 98%;
B. the extract solution of step a is placed in the double-deck stock chest 3 of sorption and desorption device, by water bath with thermostatic control 7 by the extract solution constant temperature (getting a certain value in 10-30 DEG C of temperature range) in double-deck stock chest 3;
C. organic film 13 is clipped in up and down between two block plates of organic film assembly, separately by tubular ceramic film or tubular metal film 14 vertical seal in stainless steel enrichment case, by screw thread mouth, stainless steel enrichment case upper and lower side is connected with stainless steel pipes 9;
D. ON cycle pump 2, by stainless steel pipes 9 and Pressure gauge 5, extract solution is transported to membrane module 1 to adsorb, solution after absorption is by stainless steel pipes 9, Pressure gauge 5 and flowmeter 6 are transported in double-deck stock chest 3, the pressure regulating Pressure gauge 5 is 0.25Mpa, and crossflow velocity is adsorb under 2m/s condition, pours in double-deck stock chest 3 in adsorption process every 10min by the penetrating fluid in penetrating fluid stock chest 4, after circulation 24h, protide active ingredient adsorption reaches balance;
E. from double-deck stock chest 3, sample 10ml, adopt the solution concentration after determined by ultraviolet spectrophotometry absorption, compare with the concentration of extract solution before absorption, obtain adsorbance;
F. organic film, ceramic membrane or metal film after absorption are taken off, with distilled water, sorption and desorption device is cleaned up, the film after absorption is reinstalled on sorption and desorption device;
G. by the pH of 2L be 8.5 cushioning liquid load in double-deck stock chest 3, got a certain value constant temperature in 10-30 DEG C of temperature range by water bath with thermostatic control 7;
H. at constant temperature, pressure is less than 0.05MPa and crossflow velocity carries out desorb 30min-1.5h under 0.1-1m/s range of condition, in stripping liquid, the concentration of active component is constant;
I. from double-deck stock chest 3, get stripping liquid sample, measure the concentration of stripping liquid according to the assay method in step e, obtain desorption efficiency 90%-99%.
The method of above-mentioned sorption and desorption, is characterized in that the Chinese medicine protide active component described in step a is water miscible.
The method of above-mentioned sorption and desorption, is characterized in that activated protein constituents is haemocyanin, Isin glue collagen, soybean protein.
The method of above-mentioned sorption and desorption, is characterized in that the cushioning liquid described in step g is sodium acid carbonate and sodium carbonate buffer.
the invention has the beneficial effects as follows:
The present invention effectively can solve film and refine in Chinese traditional medicine water extract process, and the active ingredient rate of transform occurred in the process of parenteral solution pyrogen and resin is low, problem of resource waste especially to adopt membrane technology to remove, and the rate of recovery can reach 99%, and film obtains regeneration preferably simultaneously.The method was both applicable to small-scale production, also met the demand of large scale business enterprise.In addition the method flow process is short, simple to operate, and according to the size of membrane module, desorption apparatus is easy to amplify, and also can echo on original production line, realize online desorb and recovery.For employing embrane method except the middle pyrogen of liquid medicine protide parenteral solution and the large-scale promotion of resin process provide technical guarantee.
four. accompanying drawing explanation
fig. 1 is the structural representation of organic film sorption and desorption device;
fig. 2 is the structural representation of ceramic membrane or metal film sorption and desorption device;
fig. 3 is organic film assembly front view.
reference numeral:1-membrane module; 2-circulating pump; 3-double-deck stock chest; 4-penetrating fluid stock chest; 5-pressure gauge; 6-flowmeter; 7-water bath with thermostatic control; 8-peristaltic pump; 9-stainless steel pipes; The import of 10-chuck; 11-JO; 12-rubber tubing; 13-organic film; 14-tubular ceramic film or tubular metal film;
15-upper metallic plate; 16-import; 17-outlet; 18-lower metallic plate; 19-circular stirrup.
five. detailed description of the invention
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1: a kind of sorption and desorption device of acid active Chinese drug component proteinaceous components film, it is characterized in that this device is by membrane module 1, circulating pump 2, double-deck stock chest 3, penetrating fluid stock chest 4, Pressure gauge 5, flowmeter 6, water bath with thermostatic control 7, peristaltic pump 8, stainless steel pipes 9 is made, wherein double-deck stock chest 3 is connected with circulating pump 2 by stainless steel pipes 9, be connected with Pressure gauge 5 by stainless steel pipes 9 again, be connected with membrane module 1 by stainless steel pipes 9 again, again by stainless steel pipes 9 and Pressure gauge 5, again by stainless steel pipes 9 and flowmeter 6, be connected with double-deck stock chest 3 by stainless steel pipes 9 again, a penetrating fluid conveying stainless steel pipes is had in membrane module 1 lower end, penetrating fluid stock chest 4 is placed below stainless steel pipes, water bath with thermostatic control 7 is connected with peristaltic pump 8 by silica gel hose 12, be connected by the import 10 of the chuck of silica gel hose 12 and extract or the double-deck stock chest 3 of stripping liquid again, the outlet 11 of the chuck of double-deck stock chest 3 is connected with water bath with thermostatic control 7 by silica gel hose 12.
The sorption and desorption device of described Chinese medicine protide active component film, it is characterized in that described membrane module 1 is divided into organic plate film assembly, ceramic film component and metal film assembly, wherein organic plate film assembly is clipped in by organic film 13 between upper and lower two pieces of corrosion resistant plates, wherein corrosion resistant plate is inverted plate-like above, the Pan Bi of axis has two holes, the short steel pipes with screw thread mouth is welded respectively in the position in hole, be connected with stainless steel pipes 9 respectively, the surface design of corrosion resistant plate has 6-12 road cast groove below, per pass cast groove is all designed with a permeate flow and portals, all stainless steel pipes 9 is carried to be connected with the penetrating fluid bottom corrosion resistant plate below, upper and lower two steel plates add tightening seal by circular iron hoop, ceramic film component or metal film assembly be by tubular ceramic film or tubular metal film 14 by upper-lower seal circle vertical seal in stainless steel enrichment case, stainless steel enrichment case upper and lower side is equipped with screw thread mouth, be connected with stainless steel pipes 9, there is a penetrating fluid delivery outlet lower end, side of stainless steel enrichment case, penetrating fluid, by stainless steel pipes 9, flows in penetrating fluid stock chest 4.
Wherein:
Circulating pump: the happy chemical pumping of German prestige, model PM-100PE, power output 100W; H-Max: 4.5m; Flow: 2.7m
3/ h.
Peristaltic pump: German Thomas's peristaltic pump, model 20251352, maximum pressure: 2.0bar; Flow: 5m3/h.
Stainless steel pipes: wall thickness: 1cm, internal diameter: 3cm; Stainless steel tube and circulating pump, pressure gauge, membrane module, flowmeter and peristaltic pump are all with being threaded;
Double-deck stock chest: material: stainless steel, internal layer groover rot diameter: 20cm; Outer groover rot diameter 26cm; Groove is high: 40cm;
Water bath with thermostatic control: U.S. PolyScience freezes hot type circulator bath, operating temperature :-40 DEG C of ∽ 200 DEG C.
Penetrating fluid stock chest: material: stainless steel, wall thickness: 1cm, internal diameter: 20cm, groove is high: 40cm;
Pressure gauge: pressure vacuum meter YZ-100Z, measures pressure limit: 0 ~ 4Mpa;
Flowmeter: pipeline dobber flowmeter, model: LFS-15, measurement category: 6-60L/h;
Wherein membrane module:
Organic film assembly: organic film poly (ether sulfone) film, PS membrane, diameter: 20cm; Upper and lower two stainless steel clamp plates: diameter: 20cm; Thickness 1.5cm.Organic film is clipped between two clamping plate, then uses circular stirrup banding.
Ceramic membrane or metal film assembly: tubular ceramic film: silicon oxide film, oxidation titanium film, long: 22cm, internal diameter: 2cm, wall thickness: 0.15cm; Tubular metal film: SUS316 film, SLS316L film, long: 22cm, internal diameter: 2cm, wall thickness: 0.15cm; ; Cylindrical stainless steel enrichment case: wall thickness: 1cm; High: 20cm; Internal diameter: 4cm; Tubular ceramic film or tubular metal film are sealed in cylindrical stainless steel enrichment case, each 1cm in upper and lower two ends.
Embodiment 2: sorption and desorption method and the concrete steps of the sorption and desorption device of acid active Chinese drug component proteinaceous components film are as follows:
The first step is that to be configured to concentration be 3.0g/L aqueous solution 10L to the Isin glue collagen extract being 98% by the content purchased in Nanjing Ze Lang Pharmaceuticals Ltd;
Second step is the double-deck stock chest 3 said extracted liquid being placed in organic film sorption and desorption device, by water bath with thermostatic control 7 by extract constant temperature to 30 DEG C;
3rd step is for being 10,000 by molecular cut off, and diameter is that polyether sulfone (PES) film of 20cm is arranged on up and down between two block plates of membrane module 1, and by two steel plates, with circular stirrup 19 banding, two ends are connected with stainless steel pipes 9;
4th step ON cycle pump 2, regulate Pressure gauge 5, pressure is adjusted to 0.25Mpa, circulate under crossflow velocity is 2m/s and 30 DEG C condition, every 10min in the process of circulation, by the penetrating fluid in penetrating fluid stock chest 4 down in double-deck stock chest 3, after circulation 24h, the absorption of Isin glue collagen extract solution reaches balance;
5th step, for sample 10ml from double-deck stock chest 3, adopts 2010 editions pharmacopeia Lowry methods to measure protein concentration, and the protein concentration recorded in the rear sample solution of absorption is 2.8g/L, and adsorbance is 2g;
Organic film sorption and desorption device, for be taken off by poly (ether sulfone) film, cleans up with distilled water by the 6th step, is reinstalled on organic film sorption and desorption device by the poly (ether sulfone) film after absorption;
7th step be by pH be 8.5 sodium acid carbonate and sodium carbonate buffer 2 liters be placed in the double-deck stock chest 3 of organic film sorption and desorption device by water bath with thermostatic control 7 constant temperature to 30 DEG C;
8th step is at 30 DEG C, and 0.03MPa and crossflow velocity are carry out desorb 90min under the condition of 0.8m/s, and in stripping liquid, protein concentration remains unchanged;
9th step for get stripping liquid 10ml from double-deck stock chest 3, and according to the assay method in above-mentioned steps five, measuring protein concentration in stripping liquid is 0.92g/L, and desorption efficiency is 92%.
Embodiment 3: sorption and desorption method and the concrete steps of the sorption and desorption device of acid active Chinese drug component proteinaceous components film are as follows:
The first step is that to be configured to concentration be 2.0g/L aqueous solution 10L to the soybean protein extract being 98% by the content purchased in Nanjing Ze Lang Pharmaceuticals Ltd;
Second step is the double-deck stock chest 3 said extracted liquid being placed in organic film sorption and desorption device, by water bath with thermostatic control 7 by extract constant temperature to 10 DEG C;
3rd step is for being 0.5 ten thousand by molecular cut off, and diameter is that polysulfones (PS) film of 20cm is arranged on up and down between two block plates of membrane module 1, and by two steel plates, with circular stirrup 19 banding, two ends are connected with stainless steel pipes 9;
4th step ON cycle pump 2, regulate Pressure gauge 5, pressure is adjusted to 0.25Mpa, circulate under crossflow velocity is 2m/s and 10 DEG C condition, every 10min in the process of circulation, by the penetrating fluid in penetrating fluid stock chest 4 down in double-deck stock chest 3, after circulation 24h, protein solution absorption reaches balance;
5th step, for sample 10ml from double-deck stock chest 3, adopts 2010 editions pharmacopeia Lowry methods to measure protein concentration, and the protein concentration recorded in the rear sample solution of absorption is 1.9g/L, and adsorbance is 1g; ;
Organic film sorption and desorption device, for be taken off by PS membrane, cleans up with distilled water by the 6th step, is reinstalled on organic film sorption and desorption device by the PS membrane after absorption;
7th step be by pH be 8.5 sodium acid carbonate and sodium carbonate buffer 2 liters be placed in the double-deck stock chest 3 of organic film sorption and desorption device by water bath with thermostatic control 7 constant temperature to 10 DEG C;
8th step is at 10 DEG C, and 0.05MPa and crossflow velocity are carry out desorb 60min under the condition of 1m/s, and in stripping liquid, protein concentration remains unchanged;
9th step for get stripping liquid 10ml from double-deck stock chest 3, and according to the assay method in above-mentioned steps five, measuring protein concentration in stripping liquid is 0.495g/L, and desorption efficiency is 99%.
Embodiment 4: sorption and desorption method and the concrete steps of the sorption and desorption device of acid active Chinese drug component proteinaceous components film are as follows:
The first step is that to be configured to concentration be 1.0g/L aqueous solution 10L to the haemocyanin extract being 98% by the content purchased in Nanjing Ze Lang Pharmaceuticals Ltd;
Second step is the double-deck stock chest 3 said extracted liquid being placed in ceramic membrane or metal film sorption and desorption device, by water bath with thermostatic control 7 by extract constant temperature to 25 DEG C;
3rd step is for being 5,000 by molecular cut off, and length is the TiO of 22cm
2film is arranged on being sealed in cylindrical stainless steel enrichment case of membrane module 1, and stainless steel enrichment case two ends are connected with stainless steel pipes;
4th step ON cycle pump 2, regulate Pressure gauge 5, pressure is adjusted to 0.25Mpa, circulate under crossflow velocity is 2m/s and 25 DEG C condition, every 10min in the process of circulation, by the penetrating fluid in penetrating fluid stock chest 4 down in double-deck stock chest 3, after circulation 24h, protein solution absorption reaches balance;
5th step, for sample 10ml from double-deck stock chest 3, adopts 2010 editions pharmacopeia Lowry methods to measure protein concentration, and the protein concentration recorded in the rear stripping liquid of absorption is 0.8g/l, and adsorbance is 2g;
6th step is by TiO
2film takes off, and ceramic membrane or metal film sorption and desorption device is cleaned up with distilled water, by the TiO after absorption
2film is reinstalled on ceramic membrane or metal film sorption and desorption device;
7th step be by pH be 8.5 sodium acid carbonate and sodium carbonate buffer 2 liters be placed in the double-deck stock chest 3 of ceramic membrane or metal film sorption and desorption device by water bath with thermostatic control 7 constant temperature to 25 DEG C;
8th step is at 25 DEG C, and 0.01MPa and crossflow velocity are carry out desorb 60min under the condition of 0.5m/s, and in stripping liquid, protein concentration remains unchanged;
9th step for get stripping liquid 10ml from double-deck stock chest 3, and according to the assay method in above-mentioned steps five, measuring protein concentration in stripping liquid is 0.92g/L, and desorption efficiency is 92%.
Embodiment 5: desorption method and the concrete steps of the sorption and desorption device of acid active Chinese drug component proteinaceous components film are as follows:
The first step is that to be configured to concentration be 5.0g/L aqueous solution 10L to the soybean protein extract being 98% by the content purchased in Nanjing Ze Lang Pharmaceuticals Ltd;
Second step is the double-deck stock chest 3 said extracted liquid being placed in ceramic membrane or metal film sorption and desorption device, by water bath with thermostatic control 7 by extract constant temperature to 25 DEG C;
3rd step is for being 10,000 by molecular cut off, and length is the SiO of 22cm
2film is arranged on being sealed in cylindrical stainless steel enrichment case of membrane module 1, and stainless steel enrichment case two ends are connected with stainless steel pipes;
4th step ON cycle pump 2, regulate Pressure gauge 5, pressure is adjusted to 0.25Mpa, circulate under crossflow velocity is 2m/s and 25 DEG C condition, every 10min in the process of circulation, by the penetrating fluid in penetrating fluid stock chest 4 down in double-deck stock chest 3, after circulation 24h, protein solution absorption reaches balance;
5th step, for sample 10ml from double-deck stock chest 3, adopts 2010 editions pharmacopeia Lowry methods to measure protein concentration, and the protein concentration recorded in the rear stripping liquid of absorption is 4.5g/l, and adsorbance is 5g;
6th step is by SiO
2film takes off, and ceramic membrane or metal film sorption and desorption device is cleaned up with distilled water, by the SiO after absorption
2film is reinstalled on ceramic membrane or metal film sorption and desorption device;
7th step be by pH be 8.5 sodium acid carbonate and sodium carbonate buffer 2 liters be placed in the double-deck stock chest 3 of ceramic membrane or metal film sorption and desorption device by water bath with thermostatic control 7 constant temperature to 25 DEG C;
8th step is at 25 DEG C, and 0.02MPa and crossflow velocity are carry out desorb 30min under the condition of 0.7m/s, and in stripping liquid, protein concentration remains unchanged;
9th step for get stripping liquid 10ml from double-deck stock chest 3, and according to the assay method in above-mentioned steps five, measuring protein concentration in stripping liquid is 2.37g/L, and desorption efficiency is 95%.
Embodiment 6: sorption and desorption method and the concrete steps of the sorption and desorption device of acid active Chinese drug component proteinaceous components film are as follows:
The first step is that to be configured to concentration be 1.0g/L aqueous solution 10L to the haemocyanin extract being 98% by the content purchased in Nanjing Ze Lang Pharmaceuticals Ltd;
Second step is the double-deck stock chest 3 said extracted liquid being placed in ceramic membrane or metal film sorption and desorption device, by water bath with thermostatic control by extract constant temperature to 25 DEG C;
3rd step is for being 10,000 by molecular cut off, and the long SUS316 film for 22cm is sealed in the cylindrical stainless steel enrichment case of ceramic membrane or metal film assembly, and stainless steel enrichment case two ends are connected with stainless steel pipes 9;
4th step ON cycle pump 2, regulate Pressure gauge 5, pressure is adjusted to 0.25Mpa, circulate under crossflow velocity is 2m/s and 25 DEG C condition, every 10min in the process of circulation, by the penetrating fluid in penetrating fluid stock chest 4 down in double-deck stock chest 3, after circulation 24h, protein extract solution absorption reaches balance;
5th step, for sample 10ml from double-deck stock chest 3, adopts 2010 editions pharmacopeia Lowry methods to measure protein concentration, and recording protein concentration in the rear stripping liquid of absorption is 0.8g/L, and adsorbance is 2g;
Ceramic membrane or metal film sorption and desorption device, for be taken off by SUS316 film, clean up with distilled water by the 6th step, are reinstalled on ceramic membrane or metal film sorption and desorption device by the SUS316 film after absorption;
7th step be by pH be 8.5 sodium acid carbonate and sodium carbonate buffer 2 liters be placed in the double-deck stock chest 3 of ceramic membrane or metal film sorption and desorption device by water bath with thermostatic control 7 constant temperature to 30 DEG C;
8th step is at 30 DEG C, and 0.03MPa and crossflow velocity are carry out desorb 60min under the condition of 0.8m/s, and in stripping liquid, protein concentration remains unchanged;
9th step for get stripping liquid 10ml from double-deck stock chest 3, and according to the assay method in above-mentioned steps five, measure 0.99g/L in stripping liquid, desorption efficiency is 99%.
Embodiment 7: desorption method and the concrete steps of the sorption and desorption device of acid active Chinese drug component proteinaceous components film are as follows:
The first step is that to be configured to concentration be 2.0g/L aqueous solution 10L to the Isin glue collagen extract being 98% by the content purchased in Nanjing Ze Lang Pharmaceuticals Ltd;
Second step is the double-deck stock chest 3 said extracted liquid being placed in ceramic membrane or metal film sorption and desorption device, by water bath with thermostatic control by extract constant temperature to 25 DEG C;
3rd step is for being 10,000 by molecular cut off, and the long SLS316L film for 22cm is sealed in the cylindrical stainless steel enrichment case of ceramic membrane or metal film assembly, and stainless steel enrichment case two ends are connected with stainless steel pipes 9;
4th step ON cycle pump 2, regulate Pressure gauge 5, pressure is adjusted to 0.25Mpa, circulate under crossflow velocity is 2m/s and 25 DEG C condition, every 10min in the process of circulation, by the penetrating fluid in penetrating fluid stock chest 4 down in double-deck stock chest 3, after circulation 24h, protein extract solution absorption reaches balance;
5th step, for sample 10ml from double-deck stock chest 3, adopts 2010 editions pharmacopeia Lowry methods to measure protein concentration, and recording protein concentration in the rear stripping liquid of absorption is 1.8g/L, and adsorbance is 2g;
Ceramic membrane or metal film sorption and desorption device, for be taken off by SLS316L film, clean up with distilled water by the 6th step, are reinstalled on ceramic membrane or metal film sorption and desorption device by the SLS316L film after absorption;
7th step be by pH be 8.5 sodium acid carbonate and sodium carbonate buffer 2 liters be placed in the double-deck stock chest 3 of ceramic membrane or metal film sorption and desorption device by water bath with thermostatic control 7 constant temperature to 25 DEG C;
8th step is at 25 DEG C, and 0.002MPa and crossflow velocity are carry out desorb 60min under the condition of 0.1m/s, and in stripping liquid, protein concentration remains unchanged;
9th step for get stripping liquid 10ml from double-deck stock chest 3, and according to the assay method in above-mentioned steps five, measure 0.9g/L in stripping liquid, desorption efficiency is 90%.
Claims (4)
1. use the sorption and desorption device of acid active Chinese drug component proteinaceous components film to carry out a method for sorption and desorption, it is characterized in that sorption and desorption method step is as follows:
A. the extract solution 10L of compound concentration to be the content of 1-5g/L the be acid Chinese medicine protide active component of 98%;
B. the extract solution of step a is placed in the double-deck stock chest (3) of sorption and desorption device, by water bath with thermostatic control (7) extract solution in double-deck stock chest (3) is got a certain value constant temperature in 10-30 DEG C of temperature range;
C. organic film (13) is clipped in up and down between two block plates of organic film assembly, separately by tubular ceramic film or tubular metal film (14) vertical seal in stainless steel enrichment case, by screw thread mouth, stainless steel enrichment case upper and lower side is connected with stainless steel pipes (9);
D. ON cycle pump (2), by stainless steel pipes (9) and Pressure gauge (5), extract solution is transported to membrane module to adsorb, solution after absorption is by stainless steel pipes (9), Pressure gauge (5) and flowmeter (6) are transported in double-deck stock chest (3), the pressure regulating Pressure gauge (5) is 0.25MPa, crossflow velocity is adsorb under 2m/s condition, every 10min, the penetrating fluid in penetrating fluid stock chest (4) is poured in double-deck stock chest (3) in adsorption process, after circulation 24h, protide active ingredient adsorption reaches balance,
E. from double-deck stock chest (3), sample 10ml, adopt the solution concentration after determined by ultraviolet spectrophotometry absorption, compare with the concentration of extract solution before absorption, obtain adsorbance;
F. organic film, ceramic membrane or metal film after absorption are taken off, with distilled water, sorption and desorption device is cleaned up, the film after absorption is reinstalled on sorption and desorption device;
G. by the pH of 2L be 8.5 cushioning liquid load in double-deck stock chest (3), got a certain value constant temperature in 10-30 DEG C of temperature range by water bath with thermostatic control (7);
H. at constant temperature, pressure is less than 0.05MPa and crossflow velocity carries out desorb 30min-1.5h under 0.1-1m/s range of condition, in stripping liquid, the concentration of active component is constant;
I. from double-deck stock chest (3), get stripping liquid sample, measure the concentration of stripping liquid according to the assay method in step e, obtain desorption efficiency 90%-99%.
2. the method for sorption and desorption according to claim 1, is characterized in that the Chinese medicine protide active component described in step a is water miscible.
3. the method for sorption and desorption according to claim 1, is characterized in that the cushioning liquid described in step g is sodium acid carbonate and sodium carbonate buffer.
4. the method for sorption and desorption according to claim 2, is characterized in that activated protein constituents is haemocyanin, Isin glue collagen, soybean protein.
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| CN201310192362.0A CN103272479B (en) | 2013-05-23 | 2013-05-23 | The sorption and desorption device of acid active Chinese drug component protein ingredient film and desorption method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2600159Y (en) * | 2002-11-28 | 2004-01-21 | 华南理工大学 | Electric ultrafiltration-liquation crystallization device for separating and purifying large biological molecule |
| CN1994536A (en) * | 2006-12-07 | 2007-07-11 | 华南理工大学 | Compound physical field reinforced membrane separation process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2600159Y (en) * | 2002-11-28 | 2004-01-21 | 华南理工大学 | Electric ultrafiltration-liquation crystallization device for separating and purifying large biological molecule |
| CN1994536A (en) * | 2006-12-07 | 2007-07-11 | 华南理工大学 | Compound physical field reinforced membrane separation process |
Non-Patent Citations (2)
| Title |
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| Development of an ultrasonic technique for in situ investigating the properties of deposited protein during crossflow ultrafiltration;Jianxin Li et.al;《Journal of Colloid and Interface Science》;20040929;第284卷(第1期);231 * |
| 阳极氧化铝膜过滤BSA溶液过程中的污染分析;陶玲等;《南京工业大学学报(自然科学版)》;20061030;第28卷(第05期);72-74 * |
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