CN103196879A - Laser-induced fluorescence spectrum detection device - Google Patents
Laser-induced fluorescence spectrum detection device Download PDFInfo
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- CN103196879A CN103196879A CN2013100895430A CN201310089543A CN103196879A CN 103196879 A CN103196879 A CN 103196879A CN 2013100895430 A CN2013100895430 A CN 2013100895430A CN 201310089543 A CN201310089543 A CN 201310089543A CN 103196879 A CN103196879 A CN 103196879A
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Abstract
The invention discloses a laser-induced fluorescence spectrum detection device, relating to the technical field of spectrum detection and aiming at solving the technical problems of low collecting efficiency and high demand on the form of a fluorescent material sample in the prior art. The laser-induced fluorescence spectrum detection device comprises a light source, a fluorescent probe and a grating spectrometer, wherein the fluorescent probe comprises a collimating lens, a narrow-band pass filter, an exciting light reflector, a dichroism light filter, a side pass filter and two focusing lenses; each part forms an exciting light path and a signal detection light path; the exciting light path starts from the light source, then sequentially passes through the collimating lens, the narrow-band pass filter, the exciting light reflector and the dichroism light filter, and then passes through the first focusing lens; the signal detection light path starts from the first focusing lens, then sequentially passes through the dichroism light filter, the side pass filter and the second focusing lens, and then enters the grating spectrometer. The laser-induced fluorescence spectrum detection device is especially suitable for detecting the fluorescence spectrum of fluorescent materials such as a quantum dot, water quality and jewel.
Description
Technical field
The present invention relates to spectrum detection technique, particularly relate to a kind of technology of laser-induced fluorescence spectroscopy pick-up unit.
Background technology
When the short radiation of visible light of ultraviolet light or wavelength during to some material, these materials can be launched the visible light of shades of colour and varying strength, and when light source stopped to shine, this light disappeared thereupon.This light that produces under the inducing of exciting light is called fluorescence, and the material that can send fluorescence is called fluorescent material.
By the principle of luminosity of fluorescence as can be known, the Wavelength-independent of molecular fluorescence spectrum and excitation source, only relevant with the level structure of fluorescent material itself, therefore the fluorescence spectrum that can produce according to fluorescent material, fluorescent material is carried out qualitative analysis differentiate that fluoroscopic examination plays an important role in fields such as biology, chemistry, environment at present.
Existing fluorescence spectrum pick-up unit all adopts xenon lamp, fluorescent probe, the combination of monochromator, xenon lamp wherein is as exciting light sources, fluorescent probe is used for collecting the fluorescence that produces after fluorescent material is excited, monochromator is for detection of fluorescence spectrum, (see figure 4) when carrying out fluoroscopic examination, the fluorescence that fluorescent probe is collected enters monochromator by the slit 21 on the monochromator earlier, reflex on the grating 23 by a catoptron 22 again, through reflexing on the single-point detector 25 by another catoptron 24 after the grating dispersion, because the light of different wave length leaves the angle difference of grating, control grating 23 orders are rotated, the fairing order of the different wave length after the grating dispersion is shone on the single-point detector 25, after grating 23 orders are rotated one-period, can realize the scanning to fluorescence spectrum, and then obtain fluorescence spectrum.
The defective of existing fluorescence spectrum pick-up unit is:
1) driver part that needs the configuration driven grating to rotate in the monochromator, therefore its volume, power consumption are all bigger, cause volume, the power consumption of whole device all too huge, and the time cycle of obtaining fluorescence spectrum is longer, can't realize the real-time collection of fluorescence spectrum, thereby some transient state characteristics that also can't the analysis of fluorescence signal, spectrum repeatability is also relatively poor;
2) beam intensity that produces of xenon lamp is low, of poor quality, and it is less to make that fluorescent material is excited to the molecular number of excited state, the fluorescence intensity that fluorescent material produces also a little less than, the detection sensitivity of therefore existing fluorescence spectrum pick-up unit is lower;
3) existing fluorescent probe all adopts from 0 degree angular range irradiation fluorescent material, receive fluorescence signal from the miter angle orientation, the fluorescence collecting efficiency of this collection mode is lower, the fluorescence volume that can collect is less, form to the fluorescent material sample is had relatively high expectations, the undesirable collection that can influence fluorescence signal of the form of fluorescent material sample, existing fluorescent probe mainly is intended for liquid fluorescent material sample at present, and it uses scene also mainly is the laboratory.
Summary of the invention
At the defective that exists in the above-mentioned prior art, technical matters to be solved by this invention provides a kind of fluorescence collecting efficiency height, form to the fluorescent material sample requires low laser-induced fluorescence spectroscopy pick-up unit, the technical matters that the present invention also will solve is to realize the real-time collection of fluorescence spectrum, raising fluoroscopic examination sensitivity, reduces the device overall volume, reduces the device overall power.
In order to solve the problems of the technologies described above, a kind of laser-induced fluorescence spectroscopy pick-up unit provided by the present invention comprises light source, fluorescent probe, grating spectrograph, it is characterized in that:
Described fluorescent probe comprises collimation lens, narrow-band pass filter, exciting light catoptron, dichroism optical filter, limit pass filter, and two condenser lenses;
Described two condenser lenses are respectively first condenser lens, second condenser lens, and in the both sides minute surface of each condenser lens, a side mirror face is the parallel sides minute surface, and the opposite side minute surface is for dispersing the side mirror face;
Described dichroism optical filter energy reflection wavelength is less than the light of 410nm, and the transmissive wavelength is more than or equal to the light of 410nm;
Collimation lens in described light source and the fluorescent probe, narrow-band pass filter, the exciting light catoptron, the dichroism optical filter, first condenser lens constitutes an excitation light path, the light channel structure of this excitation light path is: be starting point with the light source, the diverging light plane of incidence from collimation lens enters and passes collimation lens again, pass narrow-band pass filter more successively earlier, again after the exciting light mirror reflects, arrive the dichroism optical filter with 45 degree incident angles, again after the dichroism optical filter reflects with 45 degree emergence angle, along the direction that is parallel to the first condenser lens optical axis, enter first condenser lens from the parallel sides minute surface of first condenser lens, pass first condenser lens again;
First condenser lens in described grating spectrograph and the fluorescent probe, dichroism optical filter, limit pass filter, second condenser lens constitute a bars and detect light path, the light channel structure of this signal detection light paths is: be starting point with first condenser lens, again along the direction that is parallel to the first condenser lens optical axis, after passing dichroism optical filter, limit pass filter successively, enter second condenser lens from the parallel sides minute surface of second condenser lens, pass again that second condenser lens is laggard goes into grating spectrograph.
Further, described grating spectrograph comprises slit device, flashlight catoptron, reflective flat field grating, mirror condenser, the imageing sensor of glittering;
After described signal detection light paths is passed second condenser lens, pass the slit device earlier, again successively by the flashlight mirror reflects to the reflective flat field grating that glitters, to mirror condenser, reflex to imageing sensor by mirror condenser by the reflective flat field optical grating reflection that glitters.
Further, the incident angle of described narrow-band pass filter is 0 degree, and the optical wavelength that narrow-band pass filter allows to see through is 400～410nm.
Further, the incident angle of described limit pass filter is 0 degree, and the optical wavelength that the limit pass filter allows to see through is 421.5～900nm.
Further, described light source is that an energy emission wavelength be the laser generator of the Ultra-Violet Laser of 405 ± 1nm.
Laser-induced fluorescence spectroscopy pick-up unit provided by the invention, adopted the fluorescent probe of coaxial light path design, utilize condenser lens that exciting light is focused on the fluorescent material sample, and utilize same condenser lens to collect the fluorescence signal of backscattering simultaneously, owing to adopted coaxial light path design, can strengthen the collection of fluorescence signal by the numerical aperture of adjusting optical system, thereby improve the phosphor collection amount, have the high characteristics of fluorescence collecting efficiency, also just because of adopted coaxial light path design, require also very low to the form of fluorescent material sample.In addition, grating spectrograph adopts the reflective chromatic dispersion of flat field grating realization to fluorescence signal of glittering, adopt that imageing sensor is disposable catches full spectral wavelength fluorescence spectrum, obtain the time cycle very short (can reach 1ms) of fluorescence spectrum, can realize the real-time collection of fluorescence spectrum, the spectrum good reproducibility, utilize some transient state characteristics of analysis of fluorescence signal, can also reduce the device overall volume, reduce the device overall power, adopt the laser beam of laser generator emission, has the beam intensity height, the characteristics of good beam quality, can increase the molecular number that fluorescent material is excited to excited state after shining the fluorescent material sample, thereby strengthen the fluorescence that the fluorescent material sample produces, can improve fluoroscopic examination sensitivity.
Description of drawings
Fig. 1 is the structural representation of the laser-induced fluorescence spectroscopy pick-up unit of the embodiment of the invention;
Fig. 2 is the structural representation of the fluorescent probe in the laser-induced fluorescence spectroscopy pick-up unit of the embodiment of the invention;
Fig. 3 is the structural representation of the grating spectrograph in the laser-induced fluorescence spectroscopy pick-up unit of the embodiment of the invention;
Fig. 4 is the structural representation of existing monochromator;
Fig. 5 is the fluorescence peak curve map that utilizes the alcohol fluorescence that the laser-induced fluorescence spectroscopy pick-up unit of the embodiment of the invention records.
Embodiment
Below in conjunction with description of drawings embodiments of the invention are described in further detail, but present embodiment is not limited to the present invention, every employing analog structure of the present invention and similar variation thereof all should be listed protection scope of the present invention in.
As Fig. 1～shown in Figure 3, a kind of laser-induced fluorescence spectroscopy pick-up unit that the embodiment of the invention provides, comprise light source 1, fluorescent probe 3, grating spectrograph 4, it is characterized in that: described light source 1 is that an energy emission wavelength be the laser generator of the Ultra-Violet Laser of 405 ± 1nm;
Described fluorescent probe 3(sees Fig. 2) comprise collimation lens 31, narrow-band pass filter 32, exciting light catoptron 33, dichroism optical filter 34, limit pass filter 36, and two condenser lenses;
Described two condenser lenses are respectively first condenser lens 35, second condenser lens 37, and in the both sides minute surface of each condenser lens, a side mirror face is the parallel sides minute surface, and the opposite side minute surface is for dispersing the side mirror face;
Described grating spectrograph 4(sees Fig. 3) comprise slit device 41, flashlight catoptron 42, the reflective flat field grating 43 that glitters, mirror condenser 44, imageing sensor 45;
Described dichroism optical filter 34 energy reflection wavelengths are less than the light of 410nm, and the transmissive wavelength is more than or equal to the light of 410nm;
First condenser lens 35 in each parts in the described grating spectrograph 4 and the fluorescent probe 3, dichroism optical filter 34, limit pass filter 36, second condenser lens 37 constitutes a bars and detects light path, the light channel structure of this signal detection light paths is: be starting point with first condenser lens 35, again along the direction that is parallel to the first condenser lens optical axis, pass dichroism optical filter 34 successively, behind the limit pass filter 36, enter second condenser lens 37 from the parallel sides minute surface of second condenser lens 37, after passing second condenser lens 37 again, pass slit device 41 more successively, reflex to the reflective flat field grating 43 that glitters by flashlight catoptron 42, reflex to mirror condenser 44 by the reflective flat field grating 43 that glitters, reflex to imageing sensor 45 by mirror condenser 44.
In the embodiment of the invention, the incident angle of described narrow-band pass filter 32 is 0 degree (namely allowing incident angle is that 0 light of spending passes through), and the optical wavelength that narrow-band pass filter 32 allows to see through is 400～410nm, and the average transmittance of narrow-band pass filter 32 is greater than 87%.
In the embodiment of the invention, the incident angle of described limit pass filter 36 is 0 degree (namely allowing incident angle is that 0 light of spending passes through), and the optical wavelength that limit pass filter 36 allows to see through is 421.5～900nm, and the average transmittance of limit pass filter 36 is greater than 93%.
In the embodiment of the invention, what described imageing sensor 45 adopted is that model is the linear array CCD image sensor of ILX511B, among other embodiment of the present invention, described imageing sensor also can adopt other imageing sensor that can realize catching full spectral wavelength fluorescence spectrum.
The principle of work of the embodiment of the invention is as follows:
After the wavelength that fluorescent material sample 9 sends is collected by first condenser lens 35 more than or equal to the fluorescence of 410nm, along the direction that is parallel to the first condenser lens optical axis, pass dichroism optical filter 34 earlier, pass limit pass filter 36 again, behind the laser by limit pass filter 36 filtering remnants, pass second condenser lens 37, after 37 focusing of second condenser lens, enter grating spectrograph 4 by optical fiber by the slit device 41 on the grating spectrograph 4, the fluorescence of collecting enters grating spectrograph 4 backs and reflexes to the reflective flat field grating 43 that glitters by flashlight catoptron 42 earlier, fluorescence produces chromatic dispersion after the reflective flat field grating 43 that glitters is handled, the light formula of being reflected after the chromatic dispersion flat field grating 43 that glitters reflexes to mirror condenser 44, reflex to imageing sensor 45 by mirror condenser 44 again, catch the back by the conversion of mould electricity by imageing sensor 45, the fluorescence spectrum that forms a full spectral wavelength is sent into spectral analysis terminal 5.
The fluorescence spectrum that the embodiment of the invention is specially adapted to fluorescent materials such as quantum dot, water quality, jewelry detects.
Claims (5)
1. a laser-induced fluorescence spectroscopy pick-up unit comprises light source, fluorescent probe, grating spectrograph, it is characterized in that:
Described fluorescent probe comprises collimation lens, narrow-band pass filter, exciting light catoptron, dichroism optical filter, limit pass filter, and two condenser lenses;
Described two condenser lenses are respectively first condenser lens, second condenser lens, and in the both sides minute surface of each condenser lens, a side mirror face is the parallel sides minute surface, and the opposite side minute surface is for dispersing the side mirror face;
Described dichroism optical filter energy reflection wavelength is less than the light of 410nm, and the transmissive wavelength is more than or equal to the light of 410nm;
Collimation lens in described light source and the fluorescent probe, narrow-band pass filter, the exciting light catoptron, the dichroism optical filter, first condenser lens constitutes an excitation light path, the light channel structure of this excitation light path is: be starting point with the light source, the diverging light plane of incidence from collimation lens enters and passes collimation lens again, pass narrow-band pass filter more successively earlier, again after the exciting light mirror reflects, arrive the dichroism optical filter with 45 degree incident angles, again after the dichroism optical filter reflects with 45 degree emergence angle, along the direction that is parallel to the first condenser lens optical axis, enter first condenser lens from the parallel sides minute surface of first condenser lens, pass first condenser lens again;
First condenser lens in described grating spectrograph and the fluorescent probe, dichroism optical filter, limit pass filter, second condenser lens constitute a bars and detect light path, the light channel structure of this signal detection light paths is: be starting point with first condenser lens, again along the direction that is parallel to the first condenser lens optical axis, after passing dichroism optical filter, limit pass filter successively, enter second condenser lens from the parallel sides minute surface of second condenser lens, pass again that second condenser lens is laggard goes into grating spectrograph.
2. laser-induced fluorescence spectroscopy pick-up unit according to claim 1, it is characterized in that: described grating spectrograph comprises slit device, flashlight catoptron, reflective flat field grating, mirror condenser, the imageing sensor of glittering;
After described signal detection light paths is passed second condenser lens, pass the slit device earlier, again successively by the flashlight mirror reflects to the reflective flat field grating that glitters, to mirror condenser, reflex to imageing sensor by mirror condenser by the reflective flat field optical grating reflection that glitters.
3. laser-induced fluorescence spectroscopy pick-up unit according to claim 1 and 2 is characterized in that: the incident angle of described narrow-band pass filter is 0 degree, and the optical wavelength that narrow-band pass filter allows to see through is 400～410nm.
4. laser-induced fluorescence spectroscopy pick-up unit according to claim 1 and 2 is characterized in that: the incident angle of described limit pass filter is 0 degree, and the optical wavelength that the limit pass filter allows to see through is 421.5～900nm.
5. laser-induced fluorescence spectroscopy pick-up unit according to claim 1 and 2 is characterized in that: described light source is one can emission wavelength be the laser generator of the Ultra-Violet Laser of 405 ± 1nm.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104614363A (en) * | 2015-01-29 | 2015-05-13 | 上海如海光电科技有限公司 | Raman spectrum testing system based on liquid core waveguide |
| CN106290267A (en) * | 2015-05-18 | 2017-01-04 | 北京怡成生物电子技术股份有限公司 | Fluorescence detection device |
| CN106290432A (en) * | 2015-05-29 | 2017-01-04 | 中国科学院上海硅酸盐研究所 | A kind of low-temperatureX-ray induction thermoluminescence spectral measurement device |
| CN106353294A (en) * | 2016-11-09 | 2017-01-25 | 武汉能斯特科技有限公司 | Miniature capillary luminoscope |
| CN107807236A (en) * | 2017-10-31 | 2018-03-16 | 南京先进激光技术研究院 | One-color fluorescence detection means |
| CN109406478A (en) * | 2018-12-29 | 2019-03-01 | 上海市激光技术研究所 | Based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device and method |
| CN110412003A (en) * | 2019-08-28 | 2019-11-05 | 深圳技术大学 | A kind of portable gemstone fluorescence analyzer |
| CN111239090A (en) * | 2020-02-13 | 2020-06-05 | 华东师范大学重庆研究院 | Method and system for measuring single-pulse laser-induced transient molecular fluorescence spectrum |
| CN114559636A (en) * | 2022-03-10 | 2022-05-31 | 闽都创新实验室 | Method and test system capable of monitoring optical performance of quantum dot color master batch in real time |
| CN109406478B (en) * | 2018-12-29 | 2024-05-31 | 上海市激光技术研究所有限公司 | Laser-induced fluorescence spectrum detection device based on liquid lens automatic focusing and use method thereof |
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| CN109406478B (en) * | 2018-12-29 | 2024-05-31 | 上海市激光技术研究所有限公司 | Laser-induced fluorescence spectrum detection device based on liquid lens automatic focusing and use method thereof |
| CN110412003A (en) * | 2019-08-28 | 2019-11-05 | 深圳技术大学 | A kind of portable gemstone fluorescence analyzer |
| CN111239090A (en) * | 2020-02-13 | 2020-06-05 | 华东师范大学重庆研究院 | Method and system for measuring single-pulse laser-induced transient molecular fluorescence spectrum |
| CN114559636A (en) * | 2022-03-10 | 2022-05-31 | 闽都创新实验室 | Method and test system capable of monitoring optical performance of quantum dot color master batch in real time |
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Application publication date: 20130710 |