CN103098762B - Sterile cultivation method of mealworms - Google Patents
Sterile cultivation method of mealworms Download PDFInfo
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- CN103098762B CN103098762B CN201110360999.7A CN201110360999A CN103098762B CN 103098762 B CN103098762 B CN 103098762B CN 201110360999 A CN201110360999 A CN 201110360999A CN 103098762 B CN103098762 B CN 103098762B
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Abstract
The invention relates to a sterile cultivation method of mealworms. The operation steps mainly comprise processing of early generation mealworm materials, preparation of sterile bran culture media, sterile cultivation of the mealworms, and detection of sterile imagoes and quantity statistics. Through using of the provided sterile cultivation method of the mealworms, purposes that imago bodies do not carry germs, and the mealworms grow healthily and strongly are achieved. The sterile cultivation method of the mealworms is easy and convenient to operate, good in effects, and capable of being applied to material drawing experiments of sterile materials each stage of the life history of the mealworms.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of method of aseptic culture yellow mealworm.
Background technology
Yellow mealworm (Tenebrio molitor L.) classification belongs to coleoptera, intend Paussidae, intend ground beetle race, intend ground beetle genus.It is a kind of large-scale storage pest, is again a kind of resource insect of great exploitation potential for its simultaneously.This worm is mainly used as the feed of bird and some other special economic animal (scorpion, gekko, bullfrog, wood frog, soft-shelled turtle, scorpion, snake, centipede, pet fish and birds etc.), simultaneously also for developing health food or medicine (Bai Yaoyu, Cheng Jiaan, insect knowledge, 2003,40 (4): 317-322).
Yellow mealworm feeding habits are assorted, food source is wide, vitality is strong, be easy to artificial feeding, and it is a kind of desirable material that the trapping insects and infecting as disease fungus is tested.Jia Chunsheng etc. utilize yellow mealworm be separated soil insect disease fungus (Jia Chunsheng, by scholar river, Gao Wentao, insect knowledge, 2006,43 (2): 260-261), total recall rate of result insect pathogenic fungus is better than with greater wax moth method for enticing collection trapping insects disease fungus; Lee understands equality and lures collection method to be separated beauveria bassiana (Li Huiping, Huang great Zhuan, WANG Xiaohong, Zheng Jianwei, silkworm industry science, 2006,32 (3): 320-323) with yellow mealworm, also obtains good effect.Use sensitive insect to lure the insect pathogenic fungus obtained in soil, or measure the pathogenicity of insect pathogenic fungus, be easier to obtain pathogenic strong biocontrol microorganisms.But this method usually can be subject to the restriction for the supply of examination insect.
Utilizing yellow mealworm to trap in the practice of pathogenic soil fungi, the self-contained germ of yellow mealworm, also can cause yellow mealworm dead; In the experiment of pathogenicity measuring insect pathogenic fungus, the self-contained germ of yellow mealworm also can the accuracy of interference experiment.For avoiding the pathogenic dead or interference experiment result of the self-contained germ of yellow mealworm, need to eliminate the self-contained germ of yellow mealworm.The present invention adopts the method for aseptic culture yellow mealworm, builds aseptic yellow mealworm material, may be used on drawing materials of yellow mealworm each stage sterilizable material history of life and requires in experiment.
Summary of the invention
The invention provides a kind of method of aseptic culture yellow mealworm.
The operating procedure of the method is as follows:
(1) just for the process of yellow mealworm material: the Yellow meal worm larva choosing robust growth, surface clean is carried out 3 times with sterile water, the moisture on polypide is blotted with aseptic filter paper sheet, sterilize in 75% ethanol for disinfection 5-15 second, in aseptic filter paper sheet Back stroke dry wine essence, the larva handled well is as just for material.
(2) aseptic bran mass preparation: get 30g wheat bran and be contained in 500ml Cans, seal bottleneck, in 0.11MPa, 121 DEG C of sterilizing 60min, cool for subsequent use.
(3) yellow mealworm aseptic culture: by the first aseptic bran mass Cans being placed in step (2) for yellow mealworm by the amount of 10 every bottle respectively prepared in step (1), 15-25 DEG C is cultured to larvae pupation.Pupa sterile working is transferred in fresh sterile bran mass, transfer in fresh sterile bran mass after pupa is sprouted wings, the adult of post-coitum is transferred in aseptic iron wire mesh screen, sieve underneath aseptic paper, and the ovum that collection adult gives birth to is first filial generation ovum.First filial generation ovum is placed in aseptic bran mass to be continued to be cultured to adult, screens aseptic polypide, uses the same method and lay eggs, and is second filial ovum.So by generation dilution, second filial ovum is cultured to adult, and the aseptic rate detected reaches 90%; F3 ovum is cultured to adult, detect aseptic rate reach 95%; Son four generation ovum be cultured to adult, detect aseptic rate reach 100%.The aseptic polypide obtained can experimentally need, and continues the worm age being cultured to experiment needs.
(4) detection of aseptic adult and quantity statistics: by the yellow mealworm adult of the firm emergence of aseptic culture respectively sterile working be transferred in the aseptic fresh bran mass of water content 50%, cultivate 10 days for 25 DEG C, the polypide got up without growth of microorganism is aseptic.Add up aseptic adult quantity, calculate aseptic rate.
Key point of the present invention is: Yellow meal worm larva sterilization is wanted fully, but needs to control well disinfecting time, avoids overlong time, is killed by larva; Cultivation temperature can affect yellow mealworm growth, and suitable cultivation temperature can obtain healthy and strong yellow mealworm material; Aseptic acquisition is aseptic and enviromental conditions for spawning aseptic by raising medium, and constantly dilution polypide itself carried disease germs, so cultivation, subculture, the process sterile working of laying eggs will be strictly.Above key point if get hold of, the yellow mealworm filial generation ovum that can secure good health aseptic.
The present invention is easy and simple to handle, respond well, may be used in the experiment that the aseptic culture yellow mealworm history of life in each stage draws materials.
Embodiment
Following instance for illustration of the present invention, but is not limitation of the present invention.
Embodiment 1:
(1) just for the process of yellow mealworm material: the Yellow meal worm larva choosing robust growth, surface clean is carried out 3 times with sterile water, the moisture on polypide is blotted immediately with aseptic filter paper sheet, sterilized in 75% ethanol for disinfection for 5 seconds, in aseptic filter paper sheet Back stroke dry wine essence, the larva handled well is as parent material.
(2) aseptic bran mass preparation: get 30g wheat bran and be contained in 500ml Cans, seal bottleneck, in 0.11MPa, 121 DEG C of sterilizing 60min, cool for subsequent use.
(3) yellow mealworm aseptic culture: be just placed in aseptic bran mass Cans for yellow mealworm respectively by the amount of 10 every bottle by what prepare, 15 DEG C are cultured to larvae pupation.Pupa sterile working is transferred in fresh sterile bran mass, transfer in fresh sterile bran mass after pupa is sprouted wings, the adult of post-coitum is transferred in aseptic iron wire mesh screen, sieve underneath aseptic paper, and the ovum that collection adult gives birth to is first filial generation ovum.First filial generation ovum, is placed in aseptic bran mass, is cultured to the worm age that experiment needs.
(4) yield of adding up aseptic adult is 60%, than only improving 44% through sterile water polypide surface-treated control group yield.
Embodiment 2:
(1) just for the process of yellow mealworm material: the Yellow meal worm larva choosing robust growth, surface clean is carried out 3 times with sterile water, the moisture on polypide is blotted immediately with aseptic filter paper sheet, sterilized in 75% ethanol for disinfection for 10 seconds, in aseptic filter paper sheet Back stroke dry wine essence, the larva handled well is as parent material.
(2) aseptic bran mass preparation: get 30g wheat bran and be contained in 500ml Cans, seal bottleneck, in 0.11MPa, 121 DEG C of sterilizing 60min, cool for subsequent use.
(3) yellow mealworm aseptic culture: be just placed in aseptic bran mass Cans for yellow mealworm respectively by the amount of 10 every bottle by what prepare, 20 DEG C are cultured to larvae pupation.Pupa sterile working is transferred in fresh sterile bran mass, transfer in fresh sterile bran mass after pupa is sprouted wings, the adult of post-coitum is transferred in aseptic iron wire mesh screen, sieve underneath aseptic paper, and the ovum that collection adult gives birth to is first filial generation ovum.First filial generation ovum, is placed in aseptic bran mass, is cultured to the worm age that experiment needs.
(4) yield of adding up aseptic adult is 75%, than only improving 59% through sterile water polypide surface-treated control group yield.
Embodiment 3:
(1) just for the process of yellow mealworm material: the Yellow meal worm larva choosing robust growth, surface clean is carried out 3 times with sterile water, the moisture on polypide is blotted immediately with aseptic filter paper sheet, sterilized in 75% ethanol for disinfection for 15 seconds, in aseptic filter paper sheet Back stroke dry wine essence, the larva handled well is as just for material.
(2) aseptic bran mass preparation: get 30g wheat bran and be contained in 500ml Cans, seal bottleneck, in 0.11MPa, 121 DEG C of sterilizing 60min, cool for subsequent use.
(3) yellow mealworm aseptic culture: be just placed in aseptic bran mass Cans for yellow mealworm respectively by the amount of 10 every bottle by what prepare, 25 DEG C are cultured to larvae pupation.Pupa sterile working is transferred in fresh sterile bran mass, transfer in fresh sterile bran mass after pupa is sprouted wings, the adult of post-coitum is transferred in aseptic iron wire mesh screen, sieve underneath aseptic paper, and the ovum that collection adult gives birth to is first filial generation ovum.First filial generation ovum, is placed in aseptic bran mass, is cultured to the worm age that experiment needs.
(4) yield of adding up aseptic adult is 98%, than only improving 82% through sterile water polypide surface-treated control group yield.
Claims (1)
1. a method for aseptic culture yellow mealworm, is characterized in that the operating procedure of the method comprises:
(1) just for the process of yellow mealworm material: the Yellow meal worm larva choosing robust growth, surface clean is carried out 3 times with sterile water, the moisture on polypide is blotted with aseptic filter paper sheet, sterilize in 75% ethanol for disinfection 5-15 second, in aseptic filter paper sheet Back stroke dry wine essence, the larva handled well is as parent material;
(2) aseptic bran mass preparation: get 30g wheat bran and be contained in 500ml Cans, seal bottleneck, in 0.11MPa, 121 DEG C of sterilizing 60min, cool for subsequent use;
(3) yellow mealworm aseptic culture: be just placed in aseptic bran mass Cans for yellow mealworm respectively by the amount of 10 every bottle by what prepare, 15-25 DEG C is cultured to larvae pupation; Pupa sterile working is transferred in fresh sterile bran mass, transfer in fresh sterile bran mass after pupa is sprouted wings, the adult of post-coitum is transferred in aseptic iron wire mesh screen, sieve underneath aseptic paper, and the ovum that collection adult gives birth to is first filial generation ovum; First filial generation ovum is placed in aseptic bran mass to be continued to be cultured to adult, screens aseptic polypide, uses the same method and lay eggs, and is second filial ovum; So by generation dilution, second filial ovum is cultured to adult, and the aseptic rate detected reaches 90%; F3 ovum is cultured to adult, and the aseptic rate detected reaches 95%; Son four generation ovum is cultured to adult, and the aseptic rate detected reaches 100%; The aseptic polypide obtained can experimentally need, and continues the worm age being cultured to experiment needs;
(4) detection of aseptic adult and quantity statistics: by the yellow mealworm adult of the firm emergence of aseptic culture respectively sterile working be transferred in the aseptic fresh bran mass of water content 50%, cultivate 10 days for 25 DEG C, the polypide got up without growth of microorganism is aseptic; Add up aseptic adult quantity.
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| CN201110360999.7A CN103098762B (en) | 2011-11-15 | 2011-11-15 | Sterile cultivation method of mealworms |
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| CN201110360999.7A CN103098762B (en) | 2011-11-15 | 2011-11-15 | Sterile cultivation method of mealworms |
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| CN103098762B true CN103098762B (en) | 2015-02-11 |
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| CN104381219B (en) * | 2014-09-28 | 2016-04-13 | 南昌大学 | A kind of yellow mealworm and goldfish circular biological raising method |
| CN105861314A (en) * | 2015-01-24 | 2016-08-17 | 保山学院 | Method for culturing Isaria cicadae Miquel by using Tenebrio molitor pupae |
| CN104920471A (en) * | 2015-05-20 | 2015-09-23 | 何文雅 | Disinfectant for feeding yellow mealworms |
| GB201514640D0 (en) | 2015-08-18 | 2015-09-30 | Univ Exeter | Method for preparation of research organisms |
| CN108849754A (en) * | 2018-05-29 | 2018-11-23 | 绍兴明煌建材科技有限公司 | A kind of method for breeding and eating method of yellow meal worm |
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| CN100399888C (en) * | 2005-12-02 | 2008-07-09 | 河南省科学院河南省发展计划委员会地理研究所 | Method for prolonging development duration of yellow meal worm |
| CN101194602B (en) * | 2006-12-05 | 2011-08-17 | 吴锋 | High-efficiency cultivation method for flour weevil |
| CN101449670A (en) * | 2007-11-30 | 2009-06-10 | 武汉亿农丰生物工程有限公司 | Yellow meal worm larva breeding method |
| CN101669457B (en) * | 2009-10-30 | 2011-11-23 | 四川农业大学 | Cultivating method of selenium-rich yellow mealworm |
| CN101796940A (en) * | 2010-05-11 | 2010-08-11 | 毛长城 | Super flour weevil culture technology |
| CN102204522A (en) * | 2011-06-29 | 2011-10-05 | 固镇县共富养殖专业合作社 | Artificial feeding method for yellow mealworms |
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