CN102987159A - Semi-artificial diet for plutella xylostella larvae, and preparation method and application of diet - Google Patents
Semi-artificial diet for plutella xylostella larvae, and preparation method and application of diet Download PDFInfo
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- CN102987159A CN102987159A CN2012105467637A CN201210546763A CN102987159A CN 102987159 A CN102987159 A CN 102987159A CN 2012105467637 A CN2012105467637 A CN 2012105467637A CN 201210546763 A CN201210546763 A CN 201210546763A CN 102987159 A CN102987159 A CN 102987159A
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Abstract
The invention relates to semi-artificial diet for plutella xylostella larvae, and a preparation method and application of the diet. The semi-artificial diet for the plutella xylostella larvae is composed of the following components according to weight percentage: 2.67%-3.19% of malt flour, 1.07%-1.82% of cabbage powder, 3.21%-3.65% of cheese powder, 3.21%-3.65% of cane sugar powder, 0.86%-1.19% of Webster salt, 0.03%-0.05% of choline chloride, 0.09%-0.18% of sorbic acid, 0.32%-0.46% of ascorbic acid, 0.02%-0.05% of compound vitamin, 0.32%-0.46% of cholesterol, 2.14%-2.28% of agar, 0.49%-0.91% of glycerol, and the balance of water. The semi-artificial diet provided by the invention needs few raw materials which are easily available and is low in cost; the preparation method is simple and only comprises the step of boiling the materials to be dissolved; a feeding process with the diet is simple and is easy to operate; and the unit feeding quantity of the larvae is high, the development of the larvae is uniform, and thus the larvae is convenient for biological experiments such as bioassay.
Description
Technical field
The present invention relates to the man-made feeds of a kind of insect, refer to especially a kind of diamondback moth larvae meridic diet and its preparation method and application.
Background technology
Diamondback moth is the important pests of brassicaceous vegetable and crop.Raise the basis that neat diamondback moth larvae is the researchs such as diamondback moth ecology, toxicity, behavior, living survey of growing in enormous quantities, at present domestic about mainly being to raise diamondback moth with wild cabbage or other natural hosts in the research of diamondback moth, natural host is raised the cost of labor height; A small amount of research is to raise with man-made feeds, and wherein import diamondback moth feed price is expensive, and the arrival time is very long; And domestic known diamondback moth man-made feeds batching is many, and process for preparation is still aobvious loaded down with trivial details, is difficult for commercialization, uses not extensive.Therefore still need the diamondback moth artificial feeding is is further researched and developed.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of diamondback moth larvae meridic diet and its preparation method and application.
For achieving the above object, the invention provides a kind of diamondback moth larvae meridic diet, described each component of diamondback moth larvae meridic diet and percentage by weight thereof are:
Malt flour: 2.67-3.19;
Wild cabbage powder: 1.07-1.82;
Cheese powder: 3.21-3.65;
Cane sugar powder: 3.21-3.65;
Webster salt: 0.86-1.19;
Choline Chloride: 0.03-0.05;
Sorbic acid: 0.09-0.18;
Ascorbic acid: 0.32-0.46;
B B-complex: 0.02-0.05;
Cholesterol: 0.32-0.46;
Agar: 2.14-2.28;
Glycerine: 0.49-0.91;
Water surplus.
The present invention also provides the preparation method of above-mentioned diamondback moth larvae meridic diet, described diamondback moth larvae meridic diet follows these steps to preparation: 1) according to prescription weighing good malt flour, wild cabbage powder, cheese powder, cane sugar powder, Webster salt, Choline Chloride, sorbic acid, ascorbic acid, B B-complex, cholesterol, (each component effect: malt flour, wild cabbage powder, cheese powder, cane sugar powder are main nutrient composition to agar, and carbohydrate, protein and dietary fiber are provided; Webster salt, Choline Chloride, ascorbic acid, B B-complex, cholesterol are auxiliary element, and it regulates the effect of metabolism, pre-disease prevention; Sorbic acid plays antisepsis; Agar plays freezing action), evenly be mixed into former powder; 2) hot water of then getting formula ratio adds wherein, the glycerine (glycerine plays moisture-keeping function) that adds again formula ratio stirs, heat to 100 ℃ at electromagnetic oven and to boil, be distributed in a plurality of crispers, the thickness of the feed of pouring into is 5mm-10mm in each crisper; 3) cover the crisper mouth with gauze, and use the cardboard lid consistent with the box open area to cover, after being condensed into bulk, put into 4 ℃ of refrigerators, refrigerate for subsequent use.
As preferred version, wherein said wild cabbage powder is 60 ℃ of oven dry of cabbage leaves, grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
As preferred version, wherein said malt flour be commercially available wheat berry vernalization to wheat self 1/2 length, after 60 ℃ of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve.
As preferred version, wherein said cheese powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
As preferred version, wherein said cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve.
As preferred version, wherein said cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
As preferred version, the granularity of wherein said Choline Chloride was the particle of 16 mesh sieves.
As preferred version, the granularity of wherein said B B-complex is 60 orders (multivitamin and minerals tablet grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve).
As preferred version, the temperature of wherein said hot water is 90-100 ℃.
As preferred version, the volume of wherein said crisper is 470 ~ 500cm
3
The present invention further provides the application of a kind of above-mentioned diamondback moth larvae meridic diet in raising diamondback moth larvae.
Diamondback moth larvae meridic diet composition must comprise the growth course desired nutritional material of diamondback moth, and the Basic Biological Characters such as feeding habit that meet diamondback moth larvae.The feed of half artificial feeding diamondback moth larvae reasonable in design should possess, necessary nutrition and rational nutritive proportion; Stimulateing appetite arranged and be conducive to the composition that diamondback moth larvae is grown; The effective ingredient of preventing and curing diseases is arranged; Good antiseptic effect is arranged.Nutrient balance is the key problem of development feed, and the imbalance of feed nutrition composition will affect the normal growth of diamondback moth larvae and grow.
The growth course of diamondback moth larvae and life habit have uniqueness and the nutritional need of self, have significant difference with the insect of other artificial feeding in nutriment kind and ratio.
The composition that the present invention is directed in the feed has been researched and developed specific preparation method, so that the physical behavior of the effective ingredient in the feed and chemical analysis are protected to greatest extent.
The present invention has following beneficial effect:
1, the meridic diet raw material is few, and the source is easy to get, and is with low cost;
2, the meridic diet preparation procedure is simple, and only need get raw material boils molten getting final product;
3, feeding process is simple, and is easy to operate;
4, unit raising worm amount is large, grows neatly, is convenient to give birth to survey and waits other biological test.
The specific embodiment
Below in conjunction with specific embodiment the present invention is further described.
Diamondback moth larvae meridic diet prescription is shown in table 1, table 2.
Table 1: diamondback moth larvae meridic diet prescription 1
Table 2: diamondback moth larvae meridic diet prescription 2
Described diamondback moth larvae meridic diet follows these steps to preparation: 1) according to the good malt flour of prescription weighing, wild cabbage powder, cheese powder, cane sugar powder, Webster salt, Choline Chloride, sorbic acid, ascorbic acid (also claiming vitamin C), B B-complex, cholesterol, agar, evenly be mixed into former powder; 2) hot water of then getting formula ratio adds wherein, the glycerine that adds again formula ratio, stir, heat to 100 ℃ at electromagnetic oven and to boil, be distributed in the crisper (its shape can be cylinder, square or cuboid etc.), the thickness of the feed of pouring into is 5mm-10mm in each crisper; 3) cover the crisper mouth with gauze, and use the cardboard lid consistent with the box open area to cover, after being condensed into bulk, put into 4 ℃ of refrigerators, refrigerate for subsequent use.
Described wild cabbage powder is wild cabbage (rich No. one of capital) 60 ℃ of oven dry of blade, grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
Described malt flour is that commercially available wheat berry (kind is Zheng wheat 9023) vernalization grinds after 60 ℃ of oven dry to wheat self 1/2 length, crosses 80 mesh sieves, with the lower dry powder of sieve.
Described cheese powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
Described cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve.
Described cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
The granularity of described Choline Chloride was the particle (solid) of 16 mesh sieves.
The granularity of described B B-complex is that multivitamin and minerals tablet (also claiming Gold Theragran, the expensive precious pharmaceutical Co. Ltd in Shanghai) grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve.
The temperature of described hot water is 90-100 ℃.
The volume of described crisper is 470 ~ 500cm
3
Table 3: diamondback moth larvae meridic diet prescription and wild cabbage feeding effect contrast table (25 ℃)
| The test feed combination | Survival rate | Percentage of pupation | Eclosion rate | Egg laying amount (grain/head) |
| Prescription 1 | 83% | 85% | 92% | 180-210 |
| Prescription 2 | 85% | 87% | 90% | 190-220 |
| Natural feed (wild cabbage) | 82% | 90% | 95% | 160-230 |
As can be seen from Table 3, under for examination borer population same case, raise the diamondback moth larvae survival rate with diamondback moth larvae meridic diet prescription 1 and prescription 2 and be higher than natural feed (wild cabbage) 1-3%, although percentage of pupation, eclosion rate are a little less than natural feed (wild cabbage) 3-5%, but all above 85%, namely its feeding effect and natural feed (wild cabbage) are raised quite.But diamondback moth larvae meridic diet prescription is simple to operate, saves cultured space, and easily control is for the large-scale breeding of diamondback moth larvae provides indispensable condition.
Below only being the cited preferred embodiment of the present invention, is not to limit protection scope of the present invention, and the equivalence that the those of ordinary skill in the affiliated technical field uses the present invention to do is modified or changed, all with belonging to scope of patent protection of the present invention.
Claims (10)
1. a diamondback moth larvae meridic diet is characterized in that, described each component of diamondback moth larvae meridic diet and percentage by weight thereof are:
Malt flour: 2.67-3.19;
Wild cabbage powder: 1.07-1.82;
Cheese powder: 3.21-3.65;
Cane sugar powder: 3.21-3.65;
Webster salt: 0.86-1.19;
Choline Chloride: 0.03-0.05;
Sorbic acid: 0.09-0.18;
Ascorbic acid: 0.32-0.46;
B B-complex: 0.02-0.05;
Cholesterol: 0.32-0.46;
Agar: 2.14-2.28;
Glycerine: 0.49-0.91;
Water surplus.
2. the preparation method of the described diamondback moth larvae meridic diet of claim 1, it is characterized in that, described diamondback moth larvae meridic diet follows these steps to preparation: 1) according to the good malt flour of prescription weighing, wild cabbage powder, cheese powder, cane sugar powder, Webster salt, Choline Chloride, sorbic acid, ascorbic acid, B B-complex, cholesterol, agar, evenly be mixed into former powder; 2) hot water of then getting formula ratio adds wherein, adds the glycerine of formula ratio again, stirs, and heats to 100 ℃ at electromagnetic oven and boils, and is distributed in a plurality of crispers, and the thickness of pouring in each crisper is 5mm-10mm; 3) cover the crisper mouth with gauze, and use the cardboard lid consistent with the box open area to cover, after being condensed into bulk, put into 4 ℃ of refrigerators, refrigerate for subsequent use.
3. the preparation method of diamondback moth larvae meridic diet as claimed in claim 2 is characterized in that, described wild cabbage powder is 60 ℃ of oven dry of cabbage leaves, grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
4. the preparation method of diamondback moth larvae meridic diet as claimed in claim 2 is characterized in that, described malt flour be commercially available wheat berry vernalization to wheat self 1/2 length, after 60 ℃ of oven dry, grind, cross 80 mesh sieves, with the lower dry powder of sieve.
5. the preparation method of diamondback moth larvae meridic diet as claimed in claim 2 is characterized in that, the granularity of described cheese powder is that casein grinds, and crosses 80 mesh sieves, with the lower dry powder of sieve.
6. the preparation method of diamondback moth larvae meridic diet as claimed in claim 2 is characterized in that, described cane sugar powder is that sucrose grinds, and crosses 100 mesh sieves, with the lower dry powder of sieve; Described cholesterol powder is that cholesterol grinds, and crosses 20 mesh sieves, with the lower dry powder of sieve.
7. the preparation method of diamondback moth larvae meridic diet as claimed in claim 2 is characterized in that, described Choline Chloride was the particle of 16 mesh sieves; Described B B-complex is that multivitamin and minerals tablet grinds, and crosses 60 mesh sieves, with the lower dry powder of sieve.
8. the preparation method of diamondback moth larvae meridic diet as claimed in claim 2 is characterized in that, the volume of described crisper is 470 ~ 500cm
3
9. the preparation method of diamondback moth larvae meridic diet as claimed in claim 2 is characterized in that, the temperature of described hot water is 90-100 ℃.
10. the application of diamondback moth larvae meridic diet claimed in claim 1 in raising diamondback moth larvae.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478486A (en) * | 2013-09-11 | 2014-01-01 | 华南农业大学 | Diamondback moth larva pure artificial feed and preparation method thereof |
| CN105010243A (en) * | 2015-07-28 | 2015-11-04 | 武汉科诺生物科技股份有限公司 | Artificial feed for diamondback moths, and indoor artificial feeding method for diamondback moths |
| CN105146229A (en) * | 2015-10-30 | 2015-12-16 | 湖北省农业科学院植保土肥研究所 | Rice stem borer larva artificial feed and preparation method and application thereof |
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| US3974171A (en) * | 1973-10-09 | 1976-08-10 | Bayer Aktiengesellschaft | O-[3-methyl-1,3,4-triazole-(2,3,-b)-thiazol(6)yl]-(thiono)-phosphoric(phosphonic) acid esters |
| CN101347184A (en) * | 2008-09-09 | 2009-01-21 | 云南省农业科学院农业环境资源研究所 | Semi-artificial cultivated diamondback moth feed and method of preparing the same |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3974171A (en) * | 1973-10-09 | 1976-08-10 | Bayer Aktiengesellschaft | O-[3-methyl-1,3,4-triazole-(2,3,-b)-thiazol(6)yl]-(thiono)-phosphoric(phosphonic) acid esters |
| CN101347184A (en) * | 2008-09-09 | 2009-01-21 | 云南省农业科学院农业环境资源研究所 | Semi-artificial cultivated diamondback moth feed and method of preparing the same |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478486A (en) * | 2013-09-11 | 2014-01-01 | 华南农业大学 | Diamondback moth larva pure artificial feed and preparation method thereof |
| CN105010243A (en) * | 2015-07-28 | 2015-11-04 | 武汉科诺生物科技股份有限公司 | Artificial feed for diamondback moths, and indoor artificial feeding method for diamondback moths |
| CN105146229A (en) * | 2015-10-30 | 2015-12-16 | 湖北省农业科学院植保土肥研究所 | Rice stem borer larva artificial feed and preparation method and application thereof |
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