CN102955238A - Handheld fluorescence microscope - Google Patents
Handheld fluorescence microscope Download PDFInfo
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- CN102955238A CN102955238A CN2011102409349A CN201110240934A CN102955238A CN 102955238 A CN102955238 A CN 102955238A CN 2011102409349 A CN2011102409349 A CN 2011102409349A CN 201110240934 A CN201110240934 A CN 201110240934A CN 102955238 A CN102955238 A CN 102955238A
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Abstract
The invention provides a handheld fluorescence microscope. A local spectrum light source, a first optical filter, a second optical filter and an image sensing circuit are disposed in a shell of the handheld fluorescence microscope. The local spectrum light source is used for generating first light. The first optical filter is used for filtering the first light to generate second light. The second optical filter is used for filtering fluorescence generated by a sample irradiated by the second light so as to generate fourth light. The image sensing circuit is used for receiving the fourth light to generate fluorescent images. A light mask is disposed at one end of the shell and used for enclosing the sample so as to decrease or prevent light from entering the image sensing circuit from outside of the light mask. The second light irradiates an optical path of the sample, the optical path does not overlap with an optical path of the fluorescence which enters the second optical filter from the sample, and no splitter is disposed on the optical path of the fluorescence entering the second optical filter or the optical path of the fourth light entering the image sensing circuit. The handheld fluorescence microscope is capable of normally detecting fluorescent images in general environments.
Description
Technical field
The present invention is relevant for microscope, espespecially a kind of hand-held fluorescent microscope.
Background technology
Many materials can discharge the fluorescence of longer wavelength after the light that absorbs certain specific wavelength.Therefore, in experiment and research, can be by the light of check specific wavelength, and detect existence that whether a certain material is arranged in the sample or its distribution situation etc.For example, when detecting DNA, antibody or other biological specimen, can be to the light of illumination of sample specific wavelength, and the fluorescence that utilizes the fluorescence microscope sample to discharge, to detect in the sample whether test substance is arranged.
Fig. 1 is the simplification Organization Chart of existing fluorescent microscope 100, and fluorescent microscope 100 includes excitation source 110, excitation light filter (excitation filter) 120, spectroscope (dichroic mirror) 130, object lens 140, discharges light filter (emission filter) 150, reaches Image sensor apparatus 160.In fluorescent microscope 100, excitation light filter 120 is that the light L11 that excitation source 110 produces is filtered.Excitation light filter 120 can filter out the long light composition of wavelength, and (for example: light L12 490nm) is by excitation light filter 120 and make wavelength be shorter than specific wavelength.
When the light L12 after excitation light filter 120 filters shines spectroscope 130, spectroscope 130 can with wavelength particular value (for example: the light L13 500nm) below reflexes to sample, but the permission wavelength this light L14 transmission more than particular value by and do not export sample to.
The light L13 that spectroscope 130 reflects can shine sample through object lens 140.After specific fluorescent stain in the sample is excited, can discharge the fluorescence L15 of longer wavelength.Behind fluorescence L15 process object lens 140 and the spectroscope 130, can shine release light filter 150.Discharge light filter 150 and can filter out the short light composition of wavelength, and the light L16 of wavelength more than particular value (for example 510nm) passed through.Then, Image sensor apparatus 160 can produce fluoroscopic image according to the light that receives.
As shown in Figure 1, fluorescent microscope 100 light path that shines the fluorescence L15 of light L13 on the sample and Sample producing can overlap.Such optics framework meeting is so that light L13 shines the reflected light that produces behind the sample, because excitation light filter 120 and release light filter 150 limited filter effects are mingled in the light path of fluorescence L15 unfiltered reflected light, and the optical noise that increase Image sensor apparatus 160 receives, thereby the observation quality of reduction fluoroscopic image, perhaps needing to adopt the high optical filtering of filter effect and cause significantly increasing cost, is one of shortcoming of existing fluorescent microscope 100.
In addition, in existing fluorescent microscope 100, excitation source 110 is full spectrum light sources, such as high-pressure sodium lamp (mercury-vaporlamp) or high pressure deuterium lamp (xeon arc lamp) etc., so the spectral range that the light L11 that excitation source 110 produces is contained is very wide.Therefore, excitation light filter 120, spectroscope 130 and release light filter 150 all need to adopt higher specification, and the light that could reduce other wavelength components shines the interference that sample may cause, but has also therefore increased the assembly complexity of fluorescent microscope 100.
Moreover not only volume is large for aforesaid excitation source 110, and can produce high temperature in use, so need more heat-dissipating space or the complicated cooling mechanism of collocation to use, used heat could be got rid of, otherwise the life-span that can reduce the peripheral circuit assembly.Therefore, traditional excitation source 110 be difficult to fluorescent microscope 100 in other assembly combine, cause the volume of fluorescent microscope 100 all can't narrow down to the degree that can allow the user carry for a long time.So the place, place that sample all must transport first or be moved to fluorescent microscope 100 just can detect.If sample can't be prepared near the fluorescent microscope position, sample must have good preservation mechanism in transporting the way so, otherwise sample just affects the correctness of detection easily because of fluorescence decay, this is also so that the process of transporting of sample becomes one of important step that affects testing result.
Because the fluorescence that general sample discharges is very faint, so the testing process of fluorescent microscope 100 also is easy to be subject to the interference of environment light source.Therefore, existing fluorescent microscope 100 must operate in the darkroom and could obtain desirable observation image.
Above-mentioned every factor is not only so that the manufacturing of existing fluorescent microscope 100 is complicated, volume is large, cost is higher, also causes the fluorescent microscope 100 must be in specific environment (for example: the darkroom) the use restriction of operation competence exertion normal function.
Summary of the invention
In view of this, component architecture how to simplify fluorescent microscope is dwindling the size of fluorescent microscope, and the convenience that promotes the observation quality of fluoroscopic image and use, and is the industry problems to be solved in fact.
Present disclosure provides a kind of hand-held fluorescent microscope, and it includes: a housing; One first local spectroscopic light source is arranged at this enclosure interior, in order to produce one first light; One first filtering apparatus is arranged at this enclosure interior, in order to filter to provide one second light to this first light; One second filtering apparatus is arranged at this enclosure interior, in order to the same this is filtered via the rear fluorescence that produces of the second light irradiation, so that one the 4th light to be provided; One image sensing circuit is arranged at this enclosure interior, in order to receive the 4th light to produce a fluoroscopic image; And a light shield, be arranged at one of this housing end, in order to surround this sample, to reduce or to stop light outside this light shield, to enter this image sensing circuit; Wherein this second light shines the light path of this sample, can not overlap from the light path that this sample enters this second filtering apparatus with this fluorescence, and this fluorescence enters on the light path of the second filtering apparatus or the light path that the 4th light enters this image sensing circuit any spectroscope all is not set.
The present invention provides a kind of hand-held fluorescent microscope in addition, and it includes: a housing; One first local spectroscopic light source is arranged at this enclosure interior, in order to produce one first light; One first filtering apparatus is arranged at this enclosure interior, in order to filter to provide one second light to this first light; One second filtering apparatus is arranged at this enclosure interior, in order to the same this is filtered via the rear fluorescence that produces of the second light irradiation, so that one the 4th light to be provided; One second local spectroscopic light source, be arranged at this enclosure interior, shine this sample in order to produce one the 5th light, wherein the 5th light and this first light is photochromic different, and when this second local spectroscopic light source is opened, this first local spectroscopic light source can be closed, and when the first local spectroscopic light source was opened, this second local spectroscopic light source can be closed; One image sensing circuit is arranged at this enclosure interior, in order to receive the 4th light to produce a fluoroscopic image; And a light shield, be arranged at one of this housing end, in order to surround this sample, to reduce or to stop light outside this light shield, to enter this image sensing circuit; Wherein this second light shines the light path of this sample, can not overlap from the light path that this sample enters this second filtering apparatus with this fluorescence, and this fluorescence enters on the light path of the second filtering apparatus or the 4th light enters on the light path of this image sensing circuit any spectroscope all is not set.
The present invention provides a kind of hand-held fluorescent microscope in addition, and it includes: a housing; One first local spectroscopic light source is arranged at this enclosure interior, in order to produce one first light; One first filtering apparatus is arranged at this enclosure interior, in order to filter to provide one second light to this first light; One second filtering apparatus is arranged at this enclosure interior, in order to the same this is filtered via the fluorescence that the irradiation of the second light produces, so that one the 4th light to be provided; One second local spectroscopic light source, be arranged at this enclosure interior, in order to produce one the 6th light, wherein the 6th light and this first light is photochromic different, and when this second local spectroscopic light source is opened, this first local spectroscopic light source can be closed, and when the first local spectroscopic light source was opened, this second local spectroscopic light source can be closed; One the 3rd filtering apparatus is arranged at this enclosure interior, in order to the 6th light is filtered to provide one the 7th light; One image sensing circuit is arranged at this enclosure interior, in order to receive the 4th light to produce a fluoroscopic image; And a light shield, be arranged at one of this housing end, in order to surround this sample, to reduce or to stop light outside this light shield, to enter this image sensing circuit; Wherein this fluorescence enters the light path of this second filtering apparatus from this sample, can not shine the light path of this sample or the light path that the 7th light shines this sample with this second light and overlap, and this fluorescence enters on the light path of the second filtering apparatus or the 4th light enters on the light path of this image sensing circuit any spectroscope all is not set.
One of advantage of previous embodiment is that the component architecture of hand-held fluorescent microscope is simplified, and the complexity of manufacturing and cost all can be reduced.
Another advantage of previous embodiment is that local spectroscopic light source and other assembly of hand-held fluorescent microscope can be incorporated within the same housing, the volume of hand-held fluorescent microscope is significantly dwindled, and the use that is easy to carry can allow the user be convenient to detect in the various applied environments.
Another advantage of previous embodiment is that the hand-held fluorescent microscope also can normally detect fluoroscopic image in general environment, and is not limited to could use in the darkroom.
Other advantage of the present invention will be described in detail by the following description and accompanying drawing.
Description of drawings
Fig. 1 is the synoptic diagram after existing fluorescent microscope is simplified.
Fig. 2 is the synoptic diagram after one first embodiment of hand-held fluorescent microscope of the present invention simplifies.
Fig. 3 is the synoptic diagram after one second embodiment of hand-held fluorescent microscope of the present invention simplifies.
Fig. 4 is the synoptic diagram after one the 3rd embodiment of hand-held fluorescent microscope of the present invention simplifies.
Fig. 5 is the synoptic diagram after one the 4th embodiment of hand-held fluorescent microscope of the present invention simplifies.
Embodiment
Below will cooperate correlative type that embodiments of the invention are described.In these were graphic, identical label represented identical or similar assembly.
Fig. 2 is the synoptic diagram after the hand-held fluorescent microscope 200 of one embodiment of the invention is simplified.Hand-held fluorescent microscope 200 includes the first local spectroscopic light source 210, the first filtering apparatus 220, object lens 230, the second filtering apparatus 240, Image sensor apparatus 250 and housing 280, in order to the sample that is positioned on the supporting body (for example, glass sheet, desktop etc.) is carried out fluoroscopic examination.In hand-held fluorescent microscope 200, the first local spectroscopic light source 210 is to adopt the light source that volume is little and bright dipping spectrum covering scope is narrower, such as one or more various light emitting diodes (light emitting diode) or laser diode (laser diode) etc., replace the full spectrum light sources such as the larger high-pressure sodium lamp of volume or high pressure deuterium lamp, significantly to reduce the required space of light source is set.
Compared to full spectrum light sources such as existing high-pressure sodium lamp or high pressure deuterium lamps, the local spectroscopic light sources such as light emitting diode or laser diode not only volume are little a lot, and the used heat that produces when using is also far below aforesaid full spectrum light source.
When running, the first local spectroscopic light source 210 can produce the first narrower light L21 of spectrum covering scope, 220 of the first filtering apparatus can filter out the first light L21 medium wavelength greater than the light composition of one first predetermined value (for example 490nm), so that the second light L22 that only has wavelength to be less than or equal to this first predetermined value could pass through the first filtering apparatus 220.
After the second light L22 of the first filtering apparatus 220 output exposed to sample, the specific fluorescent stain in the sample can be excited and discharge the fluorescence L23 of longer wavelength (for example: more than the 515nm).In the embodiment of Fig. 2, object lens 230 are arranged on the light path between sample and the second filtering apparatus 240.Fluorescence L23 can shine directly into the second filtering apparatus 240 after by object lens 230.Here the meaning that shines directly into the second filtering apparatus 240 of saying, refer to fluorescence L23 from the light output end of object lens 230 to the light stroke that enters the second filtering apparatus 240, can be again changed light composition or the path of fluorescence L23 by other optical filterings or light-dividing device.If but can not change the light composition of fluorescence L23 or the optical element in path at object lens 230 and 240 settings of the second filtering apparatus, such as transparent glass sheet or sheet etc. then still belongs to the aspect that fluorescence L23 shines directly into the second filtering apparatus 240 after by object lens 230.
On the implementation, Image sensor apparatus 250 can adopt one or more CMOS (ComplementaryMetal Oxide Semiconductor) sensor, CCD (Charge Coupled Device) sensor, CID (Charge Injection Device) sensor, other photosensory assembly or the combination of said modules to realize, in order to produce fluoroscopic image or to carry out follow-up analysis according to the 4th light L24 by the second filtering apparatus 240.
In addition, the front end at the housing 280 of hand-held fluorescent microscope 200 also can arrange light shield 285.Light shield 285 can adopt light tight or the material of low light transmission, and the mode of assembling with one-body molded or a plurality of assemblies realizes.Light shield 285 can be set to suitable size, so that can surround sample when detecting, to block or to reduce the possibility that ambient light (such as the light L among Fig. 2) enters Image sensor apparatus 250 by light shield 285 outsides, to reduce or to get rid of ambient light to the interference of testing process.Therefore, even if aforesaid hand-held fluorescent microscope 200 also can detect fluoroscopic image smoothly under general environment, in the darkroom, could not use and be not subject to.In other words, the user will sample and hand-held fluorescent microscope 200 be carried in the darkroom and also can carry out fluoroscopic examination to sample, significantly increased selectivity and the elasticity of testing environment.
On the implementation, light shield 285 and housing 280 can combine with one-body molded mode.Perhaps, light shield 285 also available removably be bonded on the front end of housing 280.
As shown in Figure 2, the second light L22 of hand-held fluorescent microscope 200 outputs can shine directly on the sample, and the light path of the second light L22 does not overlap from the light path that sample enters the second filtering apparatus 240 with fluorescence L23.Therefore, the second light L22 of hand-held fluorescent microscope 200 outputs shines the reflected light that produces behind the sample, be difficult for being mingled in the light path that fluorescence L23 enters the second filtering apparatus 240, the optical noise that Image sensor apparatus 250 receives be can reduce, and then the observation quality of fluoroscopic image and the correctness of detection promoted.
Moreover, owing to can omit the spectroscope 130 in the existing fluorescent microscope 100 in the hand-held fluorescent microscope 200, so that required optical module comes to such an extent that simplify than prior art, and the volume of the first local spectroscopic light source 210 is also much smaller than existing full excitation of spectra light source 110, so the first local spectroscopic light source 210 in the hand-held fluorescent microscope 200, the first filtering apparatus 220, object lens 230, the assemblies such as the second filtering apparatus 240 and Image sensor apparatus 250, can adopt fixing mode or removable, or the mode of sliding is integrated in the inside of housing 280, and do not have the problem of heat radiation aspect, reach the target of fluorescent microscope miniaturization.
Thus, the user just can be carried into the sample place with hand-held fluorescent microscope 200 easily and detects.Not only promote the convenience that detects, can also shorten required sample transport program, effectively reduce the risk that sample is polluted in the process of transporting.
In addition, owing in the aforesaid hand-held fluorescent microscope 200 spectroscope 130 is not set, so the distance that object lens 230 can move between sample and Image sensor apparatus 250 becomes larger, can increase the zoom degree of freedom of hand-held fluorescent microscope 200, so that the observation quality of fluoroscopic image obtains further to promote.
In aforesaid hand-held fluorescent microscope 200, object lens 230 are arranged on the light path between sample and the second filtering apparatus 240, but this is an embodiment, but not limit to actual embodiment of the present invention.For example, in the hand-held fluorescent microscope 300 that Fig. 3 illustrates, object lens 230 are arranged on the light path between the second filtering apparatus 240 and the Image sensor apparatus 250.At this moment, fluorescence L23 can shine directly into the second filtering apparatus 240, and the 4th light L24 of the second filtering apparatus 240 outputs enters Image sensor apparatus 250 by object lens 230 again.Here the meaning that shines directly into the second filtering apparatus 240 of saying refers to fluorescence L23 and shines from sample in the light stroke of the second filtering apparatus 240, can be again by light composition or the path of other optical filterings or light-dividing device change fluorescence L23.If but can not change the light composition of fluorescence L23 or the optical element in path at sample and 240 settings of the second filtering apparatus, such as transparent glass sheet or sheet etc. then still belongs to the aspect that fluorescence L23 shines directly into the second filtering apparatus 240.
Compared to aforesaid hand-held fluorescent microscope 200, also include guiding device 360 in the hand-held fluorescent microscope 300.In the embodiments of figure 3, guiding device 360 is arranged between the light output end and sample of the first filtering apparatus 220, is used for the second light L22 of the first filtering apparatus 220 outputs is reflexed to sample.Guiding device 360 can adopt the combination of the devices such as lens set, optical fiber, light guide plate or light guiding film or aforementioned these devices to realize.By the setting of guiding device 360, the more close fluorescence L23 in position that can allow the first local spectroscopic light source 210 and the first filtering apparatus 220 to arrange is irradiated into the light path of Image sensor apparatus 250.Thus, can further reduce the required width of hand-held fluorescent microscope 300, allow the apparent size of hand-held fluorescent microscope 300 and volume energy more dwindle.On the implementation, also guiding device 360 can be changed and be arranged between the first local spectroscopic light source 210 and the first filtering apparatus 220, be used for the first light L21 with the first local spectroscopic light source 210 outputs and reflex to the first filtering apparatus 220 and filter.
In other embodiments, also multiple sets of light sources can be set simultaneously in the hand-held fluorescent microscope, to increase the operation ease of hand-held fluorescent microscope.For example, Fig. 4 is the synoptic diagram after the hand-held fluorescent microscope 400 of another embodiment of the present invention is simplified.In hand-held fluorescent microscope 400, also set up the second local spectroscopic light source 410, be used for producing photochromic the 5th different light L42 from the first light L21.For example, in one embodiment, the first local spectroscopic light source 210 is to realize that with green light LED the second local spectroscopic light source 410 then is to realize with white light LEDs.Therefore, the first light L21 that the first local spectroscopic light source 210 produces is green glow, and the 5th light L42 that the second local spectroscopic light source 410 produces then is white light.
The sample image that observes when Image sensor apparatus 250 uses the second local spectroscopic light source 410, can be when using the first local spectroscopic light source 210 to observe sample image different.In testing process, the user can utilize the switch bar (not illustrating among Fig. 4) that is arranged on the housing 280 to open first the second local spectroscopic light source 410 (and closing simultaneously the first local spectroscopic light source 210), the 5th light L42 that utilizes the second local spectroscopic light source 410 to produce comes observation sample, goes out the position that will detect in the sample or hand-held fluorescent microscope 400 is aimed at samples with Primary Location.Then, recycle this switch bar and open the first local spectroscopic light source 210 (and closing simultaneously the second local spectroscopic light source 410), the first light L21 that utilizes the first local spectroscopic light source 210 to produce comes sample is carried out fluoroscopic examination.Aforesaid light source switching mode helps the user to find sooner the sample site that will detect, and can shorten required detection time.
Please refer to Fig. 5, its illustrate is the synoptic diagram of the hand-held fluorescent microscope 500 of another embodiment of the present invention after simplifying.Hand-held fluorescent microscope 200 in Fig. 2, hand-held fluorescent microscope 500 also includes the second local spectroscopic light source 510 and the 3rd filtering apparatus 520.The second local spectroscopic light source 510 can produce one the 6th light L51, and the 3rd filtering apparatus 520 then can filter to the 6th light L51, to export one the 7th light L52.As shown in Figure 5, the output light L22 of the first filtering apparatus 220 shines the light path of sample, the light path that can not enter with the fluorescence L23 that sample discharges the second filtering apparatus 240 overlaps, and the output light L52 of the 3rd filtering apparatus 520 shines the light path of sample, also can not overlap with the light path that fluorescence L23 enter the second filtering apparatus 240.
Employed fluorescent dye and required exciting light frequency range may be different when detecting different sample.Can support more the detection to use in order to make single hand-held fluorescent microscope 500, can be with the second local spectroscopic light source 510 and the first local spectroscopic light source 210 in part embodiment both be arranged to have different go out photochromic, that is, the photochromic meeting of the first light L21 that the first local spectroscopic light source 210 produces is different from the 6th light L51 that the second local spectroscopic light source 510 produces, and the optical filtering frequency range of the 3rd filtering apparatus 520 also the optical filtering frequency range with the first filtering apparatus 220 is different.For example, the first local spectroscopic light source 210 can be the green light LED that produces green glow, and the second local spectroscopic light source 510 then can be used for producing the blue-ray LED of blue light.When the sample that detects need be blue-ray LED produce exciting light the time, the user can utilize the switch bar (not illustrating among Fig. 5) that is arranged on the housing 280 to open the second local spectroscopic light source 510 (and closing simultaneously the first local spectroscopic light source 210), provides required exciting light to utilize section of second game spectroscopic light source 510.When the sample that detects need be green light LED produce exciting light the time, the user can utilize this switch bar to open the first local spectroscopic light source 210 (and closing simultaneously the second local spectroscopic light source 510), provides required exciting light to utilize first game section spectroscopic light source 210.In other words, hand-held fluorescent microscope 500 in the present embodiment has the function of a tractor serves several purposes, can be applicable in the different fluoroscopic examination projects, need not to purchase the different fluorescent microscope of many covers with mechanism and deal with different detection needs, can significantly reduce required hardware cost.
In another embodiment, the second local spectroscopic light source 510 and both bright dipping form and aspect of the first local spectroscopic light source 210 with, and the function of the 3rd filtering apparatus 520 is also identical with the first filtering apparatus 220.When the first local spectroscopic light source 210 and the second local spectroscopic light source 510 are opened simultaneously, can promote the excitating light strength of hand-held fluorescent microscope 500 outputs, the quality of image when helping to improve the very faint sample of observation fluorescence burst size and detect correctness.
In addition, each filtering apparatus 220 in the previous embodiment, 240 and 520 can adopt respectively high pass, low pass, band logical (bandpass) or band to end absorbability (absorptive) filtering apparatus of kenels such as (bandstop) or reflectivity (reflective) filtering apparatus etc.In addition, each filtering apparatus also can carry out suitable combination with other assembly.For example, the second filtering apparatus 240 can be arranged on the object lens 230 and become single component, also the second filtering apparatus 240 can be set directly at the optical receiving end of Image sensor apparatus 250. Filtering apparatus 220 or 520 also can adopt filter directly to be arranged on local spectroscopic light source 210 or 510 in coating modes such as (coating).
By as can be known aforementioned, the output light of the hand-held fluorescent microscope among aforementioned each embodiment shines the light path of sample, can not overlap from the light path that sample enters the second filtering apparatus 240 with fluorescence.Therefore, the light of hand-held fluorescent microscope 200 outputs shines the reflected light that produces behind the sample, be difficult for being mingled in the light path that fluorescence enters the second filtering apparatus 240, the optical noise that Image sensor apparatus 250 receives be can reduce, the observation quality of fluoroscopic image and the correctness of detection promoted.
And, the framework of aforesaid hand-held fluorescent microscope need not to use spectroscope, not only can simplify the required assembly of hand-held fluorescent microscope, dwindle the volume of hand-held fluorescent microscope, also can increase the movable distance of object lens 230 between sample and Image sensor apparatus 250, so that the hand-held fluorescent microscope that the present invention proposes has better zoom degree of freedom, help the observation quality of further lifting fluoroscopic image
Because what local spectroscopic light source 210,410 and 510 adopted is the undersized light source assemblies such as light emitting diode or laser diode, and the improvement on the above-mentioned framework of arranging in pairs or groups, the volume of fluorescent microscope can more be simplified and be easy to carry about with one, and can be applied to more testing environment.
Moreover, because light shield 285 can block most ambient light outside light shield 285, can effectively reduce environment light source to fluoroscopic examination result's interference.Therefore, the user will sample and aforesaid hand-held fluorescent microscope be carried in the darkroom and also can carry out fluoroscopic examination to sample, significantly increase place selectivity and the convenience of fluoroscopic examination, for the application that enlarges fluoroscopic examination greatest help is arranged.
Some vocabulary in instructions and the claim is used to censure specific assembly, and the person of ordinary skill in the field should understand, and same assembly may be called with different nouns.This instructions and claim not with the difference of title as the mode of distinguishing assembly, but come as the benchmark of distinguishing take the difference of assembly on function.Be an open term mentioned " comprising " in instructions and claim, so should be construed to " comprise but be not limited to ".In addition, " couple " word comprise any directly and indirectly connect means.Therefore, be coupled to the second device if describe first device in the literary composition, then represent first device and can be directly connected in the second device by signal connected modes such as electric connection, wire transmission, wireless transmission or optical delivery, or by other device or connection means indirectly electrically or signal be connected to this second device.
Instructions and graphic in quantity, position and the annexation etc. of assembly only be schematically narration and draw, with simplified illustration.Each assembly can be implemented with one or more assemblies in the instructions, and perhaps the function of a plurality of assemblies also can be implemented by same assembly in the instructions, and all belongs to covering scope of the present invention.In addition, the person with usual knowledge in their respective areas will be understood that, when if some numerical value of narration is identical in instructions and the claim, for example, the numerical value such as wavelength, frequency or time, because impacts such as the error in process conditions, the design and appointed conditions, and cause these a little numerical value may be slightly different when implementing and still can reach effect of the present invention, also should belong to covering scope of the present invention.
The above only is preferred embodiment of the present invention, combination that all can be suitable between the part technical characterictic of each embodiment and each embodiment and not mutual exclusion, all equalizations of doing according to the present patent application claim change, modify and combination, all belong to covering scope of the present invention.
Claims (10)
1. hand-held fluorescent microscope includes:
One housing;
One first local spectroscopic light source is arranged at this enclosure interior, in order to produce one first light;
One first filtering apparatus is arranged at this enclosure interior, in order to filter to provide one second light to this first light;
One second filtering apparatus is arranged at this enclosure interior, in order to the same this is filtered via the rear fluorescence that produces of this second light irradiation, so that one the 4th light to be provided;
One image sensing circuit is arranged at this enclosure interior, in order to receive the 4th light to produce a fluoroscopic image; And
One light shield is arranged at one of this housing end, in order to surround this sample, to reduce or to stop light to enter this image sensing circuit outside this light shield;
Wherein this second light shines the light path of this sample, can not overlap from the light path that this sample enters this second filtering apparatus with this fluorescence, and this fluorescence enters on the light path of the second filtering apparatus or the light path that the 4th light enters this image sensing circuit any spectroscope all is not set.
2. hand-held fluorescent microscope as claimed in claim 1, other includes:
One object lens are arranged between this sample and this second filtering apparatus, or between this second filtering apparatus and this image sensing circuit.
3. hand-held fluorescent microscope as claimed in claim 2, wherein this first local spectroscopic light source is one or more light emitting diode, or one or more laser diode.
4. hand-held fluorescent microscope as claimed in claim 3, wherein this image sensing circuit can receive required electric power from a computing machine or detection system.
5. hand-held fluorescent microscope as claimed in claim 4, other includes:
One guiding device is arranged between the light output end and this sample of this first filtering apparatus, is used for this second light reflection to this sample.
6. hand-held fluorescent microscope as claimed in claim 4, other includes:
One guiding device is arranged between this first local spectroscopic light source and this first filtering apparatus, is used for this first light reflection to this first filtering apparatus.
7. hand-held fluorescent microscope includes:
One housing;
One first local spectroscopic light source is arranged at this enclosure interior, in order to produce one first light;
One first filtering apparatus is arranged at this enclosure interior, in order to filter to provide one second light to this first light;
One second filtering apparatus is arranged at this enclosure interior, in order to the same this is filtered via the rear fluorescence that produces of this second light irradiation, so that one the 4th light to be provided;
One second local spectroscopic light source, be arranged at this enclosure interior, shine this sample in order to produce one the 5th light, wherein the 5th light and this first light is photochromic different, and when this second local spectroscopic light source is opened, this first local spectroscopic light source can be closed, and when the first local spectroscopic light source was opened, this second local spectroscopic light source can be closed;
One image sensing circuit is arranged at this enclosure interior, in order to receive the 4th light to produce a fluoroscopic image; And
One light shield is arranged at one of this housing end, in order to surround this sample, to reduce or to stop light to enter this image sensing circuit outside this light shield;
Wherein this second light shines the light path of this sample, can not overlap from the light path that this sample enters this second filtering apparatus with this fluorescence, and this fluorescence enters on the light path of the second filtering apparatus or the 4th light enters on the light path of this image sensing circuit any spectroscope all is not set.
8. hand-held fluorescent microscope as claimed in claim 7, other includes:
One object lens are arranged between this sample and this second filtering apparatus, or between this second filtering apparatus and this image sensing circuit;
Wherein this first local spectroscopic light source is one or more light emitting diode, or one or more laser diode.
9. hand-held fluorescent microscope includes:
One housing;
One first local spectroscopic light source is arranged at this enclosure interior, in order to produce one first light;
One first filtering apparatus is arranged at this enclosure interior, in order to filter to provide one second light to this first light;
One second filtering apparatus is arranged at this enclosure interior, in order to the same this is filtered via the rear fluorescence that produces of this second light irradiation, so that one the 4th light to be provided;
One second local spectroscopic light source, be arranged at this enclosure interior, in order to produce one the 6th light, wherein the 6th light and this first light is photochromic different, and when this second local spectroscopic light source is opened, this first local spectroscopic light source can be closed, and when the first local spectroscopic light source was opened, this second local spectroscopic light source can be closed;
One the 3rd filtering apparatus is arranged at this enclosure interior, in order to the 6th light is filtered to provide one the 7th light;
One image sensing circuit is arranged at this enclosure interior, in order to receive the 4th light to produce a fluoroscopic image; And
One light shield is arranged at one of this housing end, in order to surround this sample, to reduce or to stop light to enter this image sensing circuit outside this light shield;
Wherein this fluorescence enters the light path of this second filtering apparatus from this sample, can not shine the light path of this sample or the light path that the 7th light shines this sample with this second light and overlap, and this fluorescence enters on the light path of the second filtering apparatus or the 4th light enters on the light path of this image sensing circuit any spectroscope all is not set.
10. hand-held fluorescent microscope as claimed in claim 9, other includes:
One object lens are arranged between this sample and this second filtering apparatus, or between this second filtering apparatus and this image sensing circuit;
Wherein this first local spectroscopic light source is one or more light emitting diode, or one or more laser diode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102409349A CN102955238A (en) | 2011-08-22 | 2011-08-22 | Handheld fluorescence microscope |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102409349A CN102955238A (en) | 2011-08-22 | 2011-08-22 | Handheld fluorescence microscope |
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| CN104865688A (en) * | 2015-06-17 | 2015-08-26 | 中国科学院半导体研究所 | Novel microscope with external fluorescent module |
| CN106443995A (en) * | 2016-11-25 | 2017-02-22 | 中国科学院上海技术物理研究所 | Optical fiber co-focus micro-imaging instrument for fluorescence observation of spatial biological sample |
| CN107101982A (en) * | 2017-03-09 | 2017-08-29 | 深圳先进技术研究院 | Fluorescence microscopy device |
| CN108037580A (en) * | 2018-01-09 | 2018-05-15 | 中山日荣塑料电子制品有限公司 | A kind of light-guiding type microscope |
| CN109116542A (en) * | 2018-11-03 | 2019-01-01 | 程昔恩 | A kind of hand-held digital microscope |
| CN112567282A (en) * | 2018-08-20 | 2021-03-26 | 美天施生物科技有限两合公司 | Microscope device |
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| US20040109231A1 (en) * | 2002-08-28 | 2004-06-10 | Carl-Zeiss-Stiftung Trading As Carl Zeiss | Microscopy system, microscopy method and a method of treating an aneurysm |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104865688A (en) * | 2015-06-17 | 2015-08-26 | 中国科学院半导体研究所 | Novel microscope with external fluorescent module |
| CN104865688B (en) * | 2015-06-17 | 2016-01-27 | 中国科学院半导体研究所 | The microscope of external fluorescent moieties |
| CN106443995A (en) * | 2016-11-25 | 2017-02-22 | 中国科学院上海技术物理研究所 | Optical fiber co-focus micro-imaging instrument for fluorescence observation of spatial biological sample |
| CN106443995B (en) * | 2016-11-25 | 2019-01-01 | 中国科学院上海技术物理研究所 | Optical fiber confocal microscopic image instrument for space biological sample Fluirescence observation |
| CN107101982A (en) * | 2017-03-09 | 2017-08-29 | 深圳先进技术研究院 | Fluorescence microscopy device |
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| CN112567282A (en) * | 2018-08-20 | 2021-03-26 | 美天施生物科技有限两合公司 | Microscope device |
| CN112567282B (en) * | 2018-08-20 | 2023-08-29 | 美天施生物科技有限两合公司 | Microscope apparatus |
| CN109116542A (en) * | 2018-11-03 | 2019-01-01 | 程昔恩 | A kind of hand-held digital microscope |
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