CN102920792B - Processed product of polygala tenuifolia with mangnolia officinalis and preparation method of same - Google Patents
Processed product of polygala tenuifolia with mangnolia officinalis and preparation method of same Download PDFInfo
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Abstract
The invention belongs to the field of medicine, and particularly relates to a processed product of polygala tenuifolia with mangnolia officinalis and a preparation method of the processed product. The invention aims at solving the technical problems of easing the side effect of polygala tenuifolia to stomach and intestine ,retaining the effects of soothing the nerves, eliminating phlegm and inducing resuscitation of polygala tenuifolia. The preparation method of the processed product provided by the invention comprises the following steps: 1, preparing mangnolia officinalis and polygala tenuifolia based on crude drug ratio in part by weight: 1-3 parts of mangnolia officinalis and 1 part of polygala tenuifolia or 2 parts of high-class mangnolia officinalis and 1 parts of polygala tenuifolia; 2, preparing mangnolia officinalis juice: mangnolia officinalis herbs are boiled in water and filtered to get mangnolia officinalis juice; and 3, processing the polygala tenuifolia with the mangnolia officinalis juice: the mangnolia officinalis juice obtained in step 1 is used to the soak polygala tenuifolia until the mangnolia officinalis juice are totally absorpt by the polygala tenuifolia, the polygala tenuifolia is roasted at the temperature of 60-180 DEG C for 6-18 minutes, and the processed product of polygala tenuifolia with mangnolia officinalis is obtained after drying. The processed product of polygala tenuifolia with mangnolia officinalis can effectively reduce the side effects of polygala tenuifolia that restrains stomach and intestine moving and retain the curative effects of relieving cough, eliminating phlegm and soothing the nerves, so as to achieve the purposes of reducing side effects and retaining curative effect. The invention has clinical application values.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product and preparation method thereof.
Background technology
Radix Polygalae is traditional Traditional Chinese medicine for tranquilization, has the merit of tranquilizing the mind, is mainly used in irritability, palpitation with fear insomnia, the forgetful treatment waiting card.Modern pharmacological research shows, Radix Polygalae extract has calmness, anticonvulsant action to central nervous system, and its root bark, full root and core are filled with to feed and given mice, have tranquilizing effect.But the toxicity of the raw product of Radix Polygalae is comparatively large, research display crude Radix Polygalae significantly can suppress digestive tract power, and hinders digestive functions, presents obvious flatulence, mucosal lesion and the side effect such as digestive enzyme is suppressed.Clinical practice safety is low, governs Radix Polygalae at clinical safety, effectively apply.
The present inventor once developed Chinese patent application 200510021948.6, and involved Radix Polygalae (processed with Mel) can reduce its gastric mucosa injury, and partial rcsponse digestive tract power suppresses.Inventor also once decocted to improve its gastrointestinal side effect together with Cortex Magnoliae Officinalis and Radix Polygalae compatibility, as " before and after Radix Polygalae and Cortex Magnoliae Officinalis compatibility chemical composition to when detoxification mechanism preliminary study " [Chengdu University of Traditional Chinese Medicine, Master's thesis in 2009], " Radix Polygalae, Cortex Magnoliae Officinalis and different ratio thereof are on the impact of the in vitro intestinal smooth muscle of rabbit " [Pharmacology and Clinics of Chinese Materia Medica the 03rd phase in 2011], " Radix Polygalae decocting time different from Cortex Magnoliae Officinalis and different ratio are on the impact of gastrointestinal motility in mice " [XI AN JIAOTONG UNIVERSITY Subject Index (medicine) the 03rd phase in 2009], specifically investigate Cortex Magnoliae Officinalis, under the different compatibility relationship of Radix Polygalae, to decoct and decocting condition together with Radix Polygalae down after Cortex Magnoliae Officinalis, on the impact of the gastrointestinal side-effect of alleviation Radix Polygalae, consequently decoct so that specific proportioning is same the gastrointestinal side-effect effectively alleviating Radix Polygalae, and Radix Polygalae " mind tranquilizing and the heart calming can be retained, eliminate the phlegm and have one's ideas straightened out " effect.
The present inventor provides a kind of brand-new Radix Polygalae processed product for improving the gastrointestinal side effect of Radix Polygalae on this basis.
Summary of the invention
Technical problem solved by the invention is Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product, for alleviating the gastrointestinal side-effect of Radix Polygalae, and retains effect of Radix Polygalae " mind tranquilizing and the heart calming eliminates the phlegm and has one's ideas straightened out ".
Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product of the present invention is prepared by following method:
A, Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio are: Cortex Magnoliae Officinalis 1-3 part, Radix Polygalae 1 part; Preferred Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B, preparation Cortex Magnoliae Officinalis juice: get magnolia medicament and decoct with water, filter to obtain Cortex Magnoliae Officinalis juice;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis juice to Radix Polygalae, process 6-18min 60-180 DEG C of stir-fry, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product.
Be below the further selection process of Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product preparation method of the present invention:
Further, in step B, the amount of water of Cortex Magnoliae Officinalis is 8-12 times amount.
Further, the time that in step B, Cortex Magnoliae Officinalis is soaked before decocting is 15-45min, preferred 30min.
Further, in step B, Cortex Magnoliae Officinalis decocting time is 5-15min, preferred 10min.
Further, soaking the time of Radix Polygalae to reduce step C Cortex Magnoliae Officinalis juice, in step B, the Cortex Magnoliae Officinalis juice after filtration can be concentrated into the 0.2-0.8 amount of Radix Polygalae, namely by Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration be condensed into 10-40ml, be convenient to absorb.
Further, in step C, temperature preferably 120 DEG C is processed in stir-fry.
Further, in step C, time preferred 12min is processed in stir-fry.
The digestive tract power that Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product of the present invention effectively can reduce crude Radix Polygalae suppresses side effect, and antitussive can be retained, eliminate the phlegm, drug effect of calming the nerves, reach and subtract the object that pair deposits effect, there is clinical value.
Accompanying drawing explanation
Fig. 1 mixing reference substance chromatogram.
Fig. 2 Radix Polygalae 20 minutes medicinal liquid negative control chromatograms.
Fig. 3 honokiol reference substance quality and peak area standard curve.
Fig. 4 magnolol reference substance quality and peak area standard curve.
The phenolic constituent chromatogram of Fig. 5 Cortex Magnoliae Officinalis 10 minutes medicinal liquid test samples.
The phenolic constituent chromatogram of Fig. 6 processed product 5 test sample.
Fig. 7 tenuifolin reference substance chromatogram.
Fig. 8 Cortex Magnoliae Officinalis 10 minutes medicinal liquid negative control chromatograms.
Fig. 9 tenuifolin reference substance quality and peak area standard curve.
The tenuifolin chromatogram of Figure 10 Radix Polygalae 20 minutes medicinal liquid test samples.
The tenuifolin chromatogram of Figure 11 processed product 5 test sample.
Detailed description of the invention
One, experimentation
1 experiment material
1.1 laboratory animal
Kunming mouse, 18 ~ 22g, is provided by Chengdu University of Traditional Chinese Medicine's Experimental Animal Center, the quality certification number: scxk (river) 2009-11.
1.2 test medicine, main agents, medicine and instrument
1.2.1 test medicine
Radix Polygalae: be purchased from Chengdu lotus pond Chinese Medicinal Materials Markets, awards through the first penetrating judgment of Chengdu University of Traditional Chinese Medicine Lu the root being accredited as Polygalaceae Radix Polygalae Polygala tenuifolia willd, belongs to 2010 editions " Chinese Pharmacopoeias " and record kind.
Cortex Magnoliae Officinalis: be purchased from Chengdu lotus pond Chinese Medicinal Materials Markets, awarding through the first penetrating judgment of Chengdu University of Traditional Chinese Medicine Lu the root bark being accredited as Cortex Magnoliae Officinalis Magnolia officinalis Rehd.et Wils. and magnolia officinalis rehd.et wils.var.biloba rehd.et wils. Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils., is that 2010 editions " Chinese Pharmacopoeias " record kind.
1.2.2 main agents, medicine
Phenol red, Chengdu Ke Long chemical reagent factory, product batch number: 0100901
Stable, Pharmaceutical Co Ltd, Changzhou Pharmaceutical Factory No.4, product batch number: 20111108
Ammonium chloride, Chengdu Ke Long chemical reagent factory, product batch number: 110308
Cisapride, Zhejiang Jingxin Pharmaceutical Co., Ltd, product batch number: 1012161
FUFANG GANCAO PIAN, company limited of Qinghai Pharmaceutic Plant, product batch number: 20111246
Pentobarbital sodium, Beijing chemical reagents corporation, product batch number: Q/H82-F158-2002
Methanol, analytical pure, Chengdu Ke Long chemical reagent factory, product batch number: 20120327
Sodium hydroxide, analytical pure, Chengdu Ke Long chemical reagent factory, product batch number: 20110921
Phosphoric acid, analytical pure, Solution on Chemical Reagents in Shanghai head factory, product batch number: 20080625
Ammonia, analytical pure, Chengdu Ke Long chemical reagent factory, product batch number: 20110418
N-butyl alcohol, analytical pure, Chengdu Ke Long chemical reagent factory, product batch number: 20110826
Pure water, Chengdu branch company of Robust (Guangdong) drinking water company limited
Chromatograph methanol, chromatographically pure, Fisher company, product batch number: 111270
Magnolol reference substance, Man Site bio tech ltd, Chengdu, purity >=98%, product batch number: 11050601
Honokiol reference substance, Man Site bio tech ltd, Chengdu, purity >=98%, product batch number: 11050602
Tenuifolin reference substance, Man Site bio tech ltd, Chengdu, purity >=98%, product batch number: 11042801
1.2.3 experimental apparatus
CP124S electronic analytical balance (SARTORIUS)
The long multi-functional readout instrument (Thermo company) of VARIOSKAN FLASH 2.4.3 all-wave
Shimadzu LC-20AT high performance liquid chromatograph (SHIAZDU company)
Microsyringe (25ul, Hamilton company)
GL-20G-II refrigerated centrifuger (Anting Scientific Instrument Factory, Shanghai)
ZZ-6 mice autonomic activities tester (Chengdu TME Technology Co., Ltd.)
Boston Green ODS C18 post (4.6 × 250mm, 5 μm,
)
1.3 experimental sites and the animal quality certification number
State Administration of Traditional Chinese Medicine of Chengdu University of Traditional Chinese Medicine three grades of herbal pharmacology laboratorys, certificate accession designation number: TCM-2009-135.The laboratory animal certificate of competency: SCXK(river) 2009-11.
2 experiment statistics software and methods
One factor analysis of variance and X 2 test is carried out by SPSS13.0 statistical software.
3 experimental techniques and result
3.1 based on the condition design of orthogonal test to Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) medicinal liquid
Adopt 7 factor 3 horizontal quadrature test cards, choose A(Radix Polygalae Cortex Magnoliae Officinalis ratio), B(Cortex Magnoliae Officinalis decocts the juice time), C(Cortex Magnoliae Officinalis juice concentrates volume), D(fries and processs temperature), E(fries and processs the time), F(Cortex et Radix Polygalae (processed) decocting time) 6 factors, draft out 18 groups of different techniques concocting medicinal liquids.
The technique that Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) concocts medicinal liquid is as follows: magnolia medicament A (n*50) g → immersion 30min → intense fire boils, slow fire boiling Bmin → be evaporated to Cml, make Cortex Magnoliae Officinalis medicine juice → add in 50g Radix Polygalae raw medicinal herbs by Cortex Magnoliae Officinalis medicine juice, moistening 3h → D degree Celsius of Radix Polygalae (parched) Emin → by fried Radix Polygalae spread out, dry → 10 times of water soaking Radix Polygalae processed product 30min → intense fires boil, slow fire boiling Fmin → be evaporated to 50ml → be settled to 100ml → process of preparing Chinese medicine medicinal liquid containing raw medicinal herbs amount 0.5g/ml.Factor, number of levels are as shown in table 1 below, and experimental establishment is as shown in table 2.
Table 16 factor 3 water-glass
Table 2 experimental establishment table
3.2 test based on Stomach residue rate and Intestinal pushing the processed product preferably alleviating gastrointestinal motility disorder
3.2.1 the preparation of medicinal liquid
Radix Polygalae medicinal liquid: 10 times of water soaking 50g Radix Polygalae raw medicinal herbs 30min → intense fires boil, slow fire boiling 60min → be evaporated to 50ml → be settled to 100ml → process of preparing Chinese medicine medicinal liquid containing raw medicinal herbs amount 0.5g/ml.
Cortex Magnoliae Officinalis medicinal liquid: 10 times of water soaking 50g Cortex Magnoliae Officinalis raw medicinal herbs 30min → intense fires boil, slow fire boiling 15min → be evaporated to 50ml → be settled to 100ml → process of preparing Chinese medicine medicinal liquid containing raw medicinal herbs amount 0.5g/ml.
Compatibility medicinal liquid: 10 times of water soaking 50g Radix Polygalae raw medicinal herbs 30min → intense fires boil, the raw magnolia medicament 100g → intense fire of slow fire boiling 45min → add 10 times amount water soaking 30min boils, slow fire boiling 15min → be evaporated to 50ml → be settled to 100ml → process of preparing Chinese medicine medicinal liquid containing raw medicinal herbs amount 1.5g/ml.
The medicinal liquid of Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): preparation such as 3.1 methods are prepared processed product 1-18 group.
3.2.2 gastric emptying and intestinal propulsion experimental technique
Get healthy Kunming mouse 300, male and female half and half, body weight 18-22g.Be divided into blank group at random by sex body weight, Radix Polygalae group, Cortex Magnoliae Officinalis group, compatibility group, concoct 1 group, concoct 2 groups, 3,4,5,6 ... 18 groups, often organize 10 (grouping and dosage refer in showing 3-7).Before experiment, water 24h is can't help in fasting, and often group gives corresponding medicinal liquid by 0.2ml/10g gavage, and blank group gives same volume normal saline.After administration 40min, gavage gives mice trophism semisolid paste, and (active carbon end 2g, stirs, is mixed with 300ml, the black semisolid pastel of about 300g for 10g CMC-Na, 250ml normal saline, milk powder 16g, sugared 8g, starch 8g.Refrigerator cold-storage, the used time returns to room temperature.) de-neck is put to death after 0.4ml/10g, 20min.
Dissect mouse peritoneal, ligation stomach cardia and pylorus, get stomach, with filter paper wipe dry after claim full weight, then cut off body of stomach along greater gastric curvature, wipe dry after washing away gastric content, claim net weight, calculate Stomach residue rate (%).
Stomach residue rate (%)=[(stomach full weight-stomach net weight)/charcoal is stuck with paste heavy] × 100%
Dissect mouse peritoneal, clip stomach pylorus is to ileocecus.Take out whole little intestinal segment without traction with tweezers, paving is straight on the plank of surface wettability, measures pylorus to charcoal and sticks with paste Distance geometry pylorus foremost to ileocecus total length, calculate Intestinal propulsive rate.
Intestinal propulsive rate (%)=(charcoal sticks with paste displacement/deep and remote blind total length) × 100%
Acquired results one factor analysis of variance is added up.
3.2.3 experimental result
Result is as shown in table 3 ~ table 7.
Table 3 Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) is on the impact (1) of Mouse Stomach residual rate and alvine pushing rate
Note: compare with blank group, * P < 0.05; Compare with Radix Polygalae group, △ P < 0.05
Table 4 Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) is on the impact (2) of Mouse Stomach residual rate and alvine pushing rate
Note: compare with Cortex Magnoliae Officinalis group, * P < 0.05, * * P < 0.01; Compare with Radix Polygalae group, △ P < 0.05, △ △ P < 0.01
Table 5 Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) is on the impact (3) of Mouse Stomach residual rate and alvine pushing rate
Note: compare with Cortex Magnoliae Officinalis group, * P < 0.05, * * P < 0.01; Compare with Radix Polygalae group, △ P < 0.05, △ △ P < 0.01
Table 6 Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) is on the impact (4) of Mouse Stomach residual rate and alvine pushing rate
Note: compare with Cortex Magnoliae Officinalis group, * * P < 0.01; Compare with Radix Polygalae group, △ P < 0.05, △ △ P < 0.01
Table 7 Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) is on the impact (5) of Mouse Stomach residual rate and alvine pushing rate
Note: compare with Cortex Magnoliae Officinalis group, * P < 0.05; Compare with Radix Polygalae group, △ P < 0.05
Result shows, and the digestive tract power that processed product 5 groups can obviously be alleviated caused by Radix Polygalae with processed product 12 groups suppresses.Therefore choose these two kinds of Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) processed products and carry out repeated experiment and relevant pharmacodynamics and investigate.
The repeated experiment that 3.3 preferred processed products affect digestive tract power
3.3.1.1 the preparation of medicinal liquid
Radix Polygalae 20 minutes medicinal liquids: 10 times of water soaking 50g Radix Polygalae raw medicinal herbs 30min → intense fires boil, slow fire boiling 20min → be evaporated to 50ml → be settled to 100ml → process of preparing Chinese medicine medicinal liquid containing raw medicinal herbs amount 0.5g/ml.
Cortex Magnoliae Officinalis 10 minutes medicinal liquids: 10 times of water soaking 50g Cortex Magnoliae Officinalis raw medicinal herbs 30min → intense fires boil, slow fire boiling 10min → be evaporated to 50ml → be settled to 100ml → process of preparing Chinese medicine medicinal liquid containing raw medicinal herbs amount 0.5g/ml.
Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) medicinal liquid 5: magnolia medicament 100g → immersion 30min → intense fire boils, slow fire boiling 10min → be evaporated to 25ml, make Cortex Magnoliae Officinalis medicine juice → add in 50g Radix Polygalae raw medicinal herbs by Cortex Magnoliae Officinalis medicine juice, moistening 3h → 120 degree Celsius Radix Polygalae (parched) 12min → by fried Radix Polygalae spread out, dry → 10 times of water soaking Radix Polygalae processed product 30min → intense fires boil, slow fire boiling 20min → be evaporated to 50ml → be settled to 100ml.
Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) medicinal liquid 12: magnolia medicament 50g → immersion 30min → intense fire boils, slow fire boiling 15min → be evaporated to 25ml, make Cortex Magnoliae Officinalis medicine juice → add in 50g Radix Polygalae raw medicinal herbs by Cortex Magnoliae Officinalis medicine juice, moistening 3h → 90 degree Celsius Radix Polygalae (parched) 6min → by fried Radix Polygalae spread out, dry → 10 times of water soaking Radix Polygalae processed product 30min → intense fires boil, slow fire boiling 20min → be evaporated to 50ml → be settled to 100ml.
3.3.1.2 experimental technique
Get healthy Kunming mouse 84, male and female half and half, body weight 18-22g.By mice sex body weight be divided at random blank group, cisapride group, Radix Polygalae 20 minutes groups, Cortex Magnoliae Officinalis 10 minutes groups, processed product 5 groups, processed products 12 groups, often organize 14., Intestinal pushing experimental technique residual according to 3.2.2 stomach is tested.
3.3.1.3 experimental result is as shown in table 8.
Table 8 Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) Mouse Stomach is remained and Intestinal pushing impact repeat experiment
Note: compare * * P < 0.01, * P < 0.05 with blank group; Compare with Radix Polygalae group, △ △ P < 0.01, △ P < 0.05
Result shows, processed product 5 groups and processed product 12 groups all significantly can reduce Mouse Stomach residual rate and increase Intestinal propulsive rate (P < 0.05), but the Stomach residue rate that processed product is 12 groups is significantly higher than blank group (P < 0.05), point out this processed product still can affect stomach motion; And processed product 5 organizes there was no significant difference (P > 0.05) with blank, illustrate that the process of preparing Chinese medicine 5 groups is closer to normal mouse.In addition, separately in pentobarbital sodium Synergism Testing, processed product 12 groups does not present drug effect of calming the nerves, therefore pharmacodynamic experiment thereafter only selects processed product 5 to study.
3.3.2.1 the preparation of medicinal liquid
1. Radix Polygalae medicinal liquid: 10 times of water soaking 50g Radix Polygalae raw medicinal herbs 30min → intense fires boil, slow fire boiling 20min → be evaporated to 50ml → be settled to 100ml → medicinal liquid containing raw medicinal herbs amount 0.5g/ml.
2. Cortex Magnoliae Officinalis medicinal liquid: 10 times of water soaking 100g Cortex Magnoliae Officinalis raw medicinal herbs 30min → intense fires boil, slow fire boiling 10min → be evaporated to 50ml → be settled to 100ml → medicinal liquid containing raw medicinal herbs amount 1.0g/ml.
3. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) medicinal liquid high dose: magnolia medicament 100g → immersion 30min → intense fire boils, slow fire boiling 10min → be evaporated to 25ml, make Cortex Magnoliae Officinalis medicine juice → add in 50g Radix Polygalae raw medicinal herbs by Cortex Magnoliae Officinalis medicine juice, moistening 3h → 120 degree Celsius Radix Polygalae (parched) 12min → by fried Radix Polygalae spread out, dry → 10 times of water soaking Radix Polygalae processed product 30min → intense fires boil, slow fire boiling 20min → be evaporated to 50ml → be settled to 100ml → medicinal liquid containing raw medicinal herbs amount 1.5g/ml.
4. compatibility medicinal liquid: 10 times of water soaking 50g Radix Polygalae raw medicinal herbs 30min → intense fires boil, Cortex Magnoliae Officinalis → the intense fire of slow fire boiling 10min → add 10 times of water soaking 100g Cortex Magnoliae Officinalis raw medicinal herbs 30min boils, slow fire boiling 10min → be evaporated to 50ml → be settled to 100ml → medicinal liquid containing raw medicinal herbs amount 1.5g/ml.
3.3.2.2 experimental technique
Get healthy Kunming mouse 84, male and female half and half, body weight 18-22g.By sex body weight be divided at random blank group, cisapride group, Radix Polygalae group, Cortex Magnoliae Officinalis group, compatibility group, concoct high dose group, often organize 14.Before experiment, water 24h is can't help in fasting, and often group gives corresponding medicinal liquid by 0.2ml/10g gavage, and blank group gives same volume normal saline.After administration 40min, gavage gives mice trophism semisolid paste, and (active carbon end 2g, stirs, is mixed with 300ml, the black semisolid pastel of about 300g for 10g CMC-Na, 250ml normal saline, milk powder 16g, sugared 8g, starch 8g.Refrigerator cold-storage, the used time returns to room temperature.) de-neck is put to death after 0.4ml/10g, 20min.
Dissect mouse peritoneal, ligation stomach cardia and pylorus, get stomach, with filter paper wipe dry after claim full weight, then cut off body of stomach along greater gastric curvature, wipe dry after washing away gastric content, claim net weight, calculate Stomach residue rate (%).
Stomach residue rate (%)=[(stomach full weight-stomach net weight)/charcoal is stuck with paste heavy] × 100%
Dissect mouse peritoneal, clip stomach pylorus is to ileocecus.Take out whole little intestinal segment without traction with tweezers, paving is straight on the plank of surface wettability, measures pylorus to charcoal and sticks with paste Distance geometry pylorus foremost to ileocecus total length, calculate Intestinal propulsive rate.
Intestinal propulsive rate (%)=(charcoal sticks with paste displacement/deep and remote blind total length) × 100%
3.3.2.3 experimental result is as shown in table 9.
Table 9 Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) remains Mouse Stomach and Intestinal pushing affects experimental result
Compare with Radix Polygalae group, △ △ P < 0.01, △ P < 0.05
Result show, compare with Radix Polygalae group, blank group, cisapride group, Cortex Magnoliae Officinalis group, compatibility group and concoct high dose group all significantly can reduce Mouse Stomach residual rate (△ △ P < 0.01, △ P < 0.05); Blank group, cisapride group, Cortex Magnoliae Officinalis group and processed product high dose group all significantly can increase mice alvine pushing rate (△ △ P < 0.01, △ P < 0.05).The gastrointestinal motility disorder that processed product high dose group significantly can be alleviated Radix Polygalae and causes is described, and effect is better than the compatibility group of parallel preparation.
3.4 processed product pentobarbital sodiums work in coordination with hypnosis and antitussive pharmacodynamic study
1. Radix Polygalae medicinal liquid: 10 times of water soaking 50g Radix Polygalae raw medicinal herbs 30min → intense fires boil, slow fire boiling 20min → be evaporated to 50mL → be settled to 100mL, simmer down to is the medicinal liquid of 0.5g/mL containing raw medicinal herbs concentration.
2. Cortex Magnoliae Officinalis medicinal liquid: 10 times of water soaking 100g Cortex Magnoliae Officinalis raw medicinal herbs 30min → intense fires boil, slow fire boiling 10min → be evaporated to 50mL → be settled to 100mL, simmer down to is the medicinal liquid of 1.0g/mL containing raw medicinal herbs concentration.
3. processed product high dose medicinal liquid: magnolia medicament 100g → immersion 30min → intense fire boils, slow fire boiling 10min → be evaporated to 25ml, make Cortex Magnoliae Officinalis medicine juice → add in 50g Radix Polygalae raw medicinal herbs by Cortex Magnoliae Officinalis medicine juice, moistening 3h → 120 degree Celsius Radix Polygalae (parched) 12min → by fried Radix Polygalae spread out, dry → 10 times of water soaking Radix Polygalae processed product 30min → intense fires boil, slow fire boiling 20min → be evaporated to 50ml → be settled to 100ml, simmer down to is the medicinal liquid of 1.5g/mL containing raw medicinal herbs concentration.
4. dosage medicinal liquid in processed product: get high dose medicinal liquid 50ml, be settled to 100ml.
5. processed product low dosage medicinal liquid: get middle dosage medicinal liquid 50ml, be settled to 100ml.
3.4.1 pentobarbital sodium is worked in coordination with to experimental technique and the result of hypnosis mice
Get healthy Kunming mouse 98, by sex body weight be divided at random blank group, diazepam group, Radix Polygalae group, Cortex Magnoliae Officinalis group, processed product high dose group, dosage group and processed product low dose group totally 7 groups in processed product, often organize 14.Before experiment, water 9h is can't help in fasting, gives corresponding medicinal liquid according to 0.2mL/10g gavage, the blank group of normal saline giving same volume.After administration 30min, the Nembutal sodium solution (150mg is made into 100mL) of the freshly prepared 30mg/kg of lumbar injection.Be sleep standard with mice more than righting reflex loss 1min.Add up mice sleep percentage rate in each group of 15min.Result is as shown in table 10.
Table 10 various dose Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) works in coordination with the impact of mice syngignoscism on pentobarbital sodium
Compare with blank group, * P < 0.05, * * P < 0.01
Result shows, and compare with blank group, in diazepam group, processed product high dose group, processed product, dosage group significantly can strengthen the effect (P < 0.05) of pentobarbital sodium being worked in coordination with to mice hypnosis.
3.4.2 to antitussive effect experimental technique and the result of the cough of ammonia induced mice
Get healthy Kunming mouse 98, by body weight be divided at random blank group, FUFANG GANCAO PIAN group, Radix Polygalae group, Cortex Magnoliae Officinalis group, processed product high dose group, dosage group and processed product low dose group totally 5 groups in processed product, often organize 14.Before experiment, water 9h is can't help in fasting, gives corresponding medicinal liquid according to 20mL/kg gavage, the blank group of normal saline giving same volume.After administration 1h, mice is placed in the wide mouthed bottle that volume is 250mL, in it, puts a cotton balls, inhale 25% ~ 28% ammonia 0.1mL with liquid-transfering gun at every turn and inject on cotton balls.That coughs shows as mice contraction of abdominal muscle, magnifies mouth simultaneously, sometimes coughs sound.Observe and the initial Cough length recorded in mice 3min and cough number of times.Result is as shown in table 11.
Table 11 various dose Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) is on the impact of the antitussive effect that ammonia induced mice is coughed
Result shows, and compares with blank group, and each administration group all can reduce the cough number of times (P < 0.01) of ammonia induced mice in pole significantly; FUFANG GANCAO PIAN group, processed product high dose group can also pole significant prolongation ammonia induced mice cough latent time (P < 0.01), the incubation period (P < 0.05) of Radix Polygalae group, Cortex Magnoliae Officinalis group energy significant prolongation ammonia induced mice cough.
3.5 various dose are on the impact of mice phlegm-dispelling functions and autonomic activities
3.5.1 various dose is on the impact of mice phlegm-dispelling functions
(1) 1% phenol red preparation
1% phenol red preparation: be in the NaOH normal saline solution of 0.04g/100ml by the concentration of phenol red for the 1g 11.28ml of being dissolved in, be ground to without after fine particle by phenol red, funnel filters, and repeats with normal saline funnel of washing one's face and rinsing one's mouth, be settled in 100ml volumetric flask, obtained final product.
(2) experimental technique and result
Get healthy Kunming mouse 112, water 9h is can't help in fasting, by body weight be divided at random blank group, Cortex Magnoliae Officinalis interference matched group, ammonium chloride group, Radix Polygalae group, Cortex Magnoliae Officinalis group, processed product high dose group, dosage group and processed product low dose group in processed product, totally 8 groups, often organize 14.By the corresponding medicinal liquid of 20mL/kg gastric infusion mice, the blank group of normal saline giving same volume.After administration 30min, except Cortex Magnoliae Officinalis interference matched group is pressed except 10mL/kg intraperitoneal injection of saline, all the other 7 groups to press 10mL/kg body weight lumbar injection 1% phenol red, mice is put to death after 30min, peel off trachea surrounding tissue, cut one section of trachea down to trachea bifurcation from thyroid cartilage, put in the test tube filling 2mL normal saline, add 0.1mL sodium hydroxide solution (1mol/L) again, the centrifugal 15min of concussion 10min, 3000rpm, get supernatant and add 96 orifice plates, measure by microplate reader, wavelength 546nm, measure absorbance (OD) value.
For getting rid of magnolol to phenol red experiment interference, ad hoc Cortex Magnoliae Officinalis interference matched group, to get rid of the interference of magnolol to specific wavelength absorbance.Experimental procedure is: by the corresponding Cortex Magnoliae Officinalis medicinal liquid of 20ml/kg gastric infusion mice, after administration 30min, by 0.1ml/10g body weight intraperitoneal injection of saline, 30min puts to death mice, and step is the same to be measured.Acquired results one factor analysis of variance is added up.Result is as shown in table 12.
Table 12 various dose Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) is on the impact of mice PSP effect
Result shows, and the OD value of Cortex Magnoliae Officinalis interference matched group compares there was no significant difference (P > 0.05) with blank group, and its OD value is lower than blank group.Compare with blank group, ammonium chloride group and processed product high dose group all significantly can increase phenol red OD value (the P < 0.05 of tracheal strips, P < 0.01), Radix Polygalae group and Cortex Magnoliae Officinalis group all have the trend higher than blank group OD value, but there are no significant difference (P > 0.05).
3.5.2 various dose is on the impact of mice autonomic activities
Get healthy Kunming mouse 98.Adaptability raises 3d, and is fixed on every day after mice is positioned over mice autonomic activities analyzer 3min by 13:00 respectively, records mice 5min activity value respectively, and activity value is movable number of times and the summation of number of times of standing.Eliminate too active or very sluggish mice, filter out the qualified mice of the conduct of mean activity value between 80 ~ 200.In experiment 4d, again measure and record mice activity value as the basic activity value before administration, and abreast mice is divided into blank group at random according to basic activity value, dosage group and processed product low dose group totally 7 groups in diazepam group, Radix Polygalae group, Cortex Magnoliae Officinalis group, processed product high dose group, processed product, often organizes 14.2d i.e. formal experiment 1d after grouping, each administration group gives mice corresponding medicinal liquid by 20mL/kg gavage, and blank group gives same volume normal saline, continuous 7d.In formal experiment 1d, 3d, 7d administration 30min, be measured in the same method the movable number of times of mice and stand number of times, as activity value after mice administration.
With the change of movable suppression ratio reflection mice autonomic activities.
Movable suppression ratio=((activity value after administration-removable foundation value)/removable foundation value) × 100%
Acquired results sas8.0 carries out Kruskal analysis.Experimental result is as shown in table 13.
Table 13 various dose Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) is on the impact of mice autonomic activities change effect
Result shows, each assembly there was no significant difference (P > 0.05) before administration.After administration, compare with blank group, diazepam group, process of preparing Chinese medicine high dose group all significantly can suppress mice autonomic activities (P < 0.05 in 1d, 3d, 7d time, P < 0.01), in processed product, dosage group can significantly suppress mice autonomic activities (P < 0.05) to present sedation at 1d, 3d and Radix Polygalae group at 3d.
The phenolic constituent assay of 3.6 Cortex Magnoliae Officinalis and Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed)
3.6.1 experiment material and instrument
Magnolol and honokiol reference substance (purity is all greater than 98.0%, Man Site bio tech ltd, Chengdu), chromatograph methanol are chromatographically pure, and other reagent such as methanol, pure water are analytical pure.
Shimadzu LC-20AT high performance liquid chromatograph (SHIAZDU company), UV detector, CP124S electronic analytical balance (SARTORIUS).
3.6.2 chromatographic condition and system suitability test
Boston Green ODS C18 chromatographic column (4.6 × 250mm, 5 μm,
); Mobile phase methanol-water (78:22) is mobile phase; Flow velocity 1mlmin
-1; Column temperature: 30 DEG C; Determined wavelength is 294nm.Separating degree is all greater than 1.5, and theoretical cam curve calculates by magnolol peak and is not less than 3800.
3.6.3 the preparation of sample
Magnolol reference substance is got in the preparation of mixing reference substance solution, honokiol reference substance is appropriate, accurately weighed, adds methanol mixed and makes the solution of every 1ml containing magnolol 40ug, honokiol 24ug, to obtain final product.Gained chromatogram as shown in Figure 1.
The preparation precision of need testing solution measures Radix Polygalae 20 minutes medicinal liquids, Cortex Magnoliae Officinalis 10 minutes medicinal liquids, each 2ml of processed product 5, adds methanol constant volume in 10ml volumetric flask, jolting.Cross 0.45um microporous filter membrane, to obtain final product.
3.6.4 negative interference test
Get Radix Polygalae test sample 10ul under 3.6.3 item, according to chromatographic condition sample introduction under 3.6.2 item, detect, do not find interference.Result illustrates that the mensuration of Radix Polygalae single medicinal liquid to Cortex Magnoliae Officinalis medicine liquid ingredient is not disturbed, and refers to Fig. 2.
3.6.5 linear relationship is investigated
Getting magnolol, honokiol mixing reference substance 2ul, 4ul, 6ul, 8ul, 10ul, 12ul, 18ul and concentration under 3.6.3 item is respectively the mixing reference substance 10ul sample introduction of every 1ml containing honokiol 48ug, magnolol 80ug, obtain the relation of its quality and peak area, calculate regression equation and R
2value, the R of honokiol and magnolol
2value is 0.9994, illustrates that linear relationship is good.The results are shown in following table 14,15 and Fig. 3,4.
The quality of table 14 honokiol and peak area relation
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| M(ug） | 0.048 | 0.096 | 0.144 | 0.192 | 0.240 | 0.288 | 0.432 | 0.480 |
| A | 42819.5 | 83818.1 | 126579.2 | 163625.3 | 201892.5 | 342478.6 | 353334.5 | 402303.3 |
The quality of table 15 magnolol and peak area relation
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| M(ug） | 0.080 | 0.160 | 0.240 | 0.320 | 0.400 | 0.480 | 0.720 | 0.800 |
| A | 64260.4 | 127922.3 | 191732.4 | 252755.8 | 314245.9 | 375578.6 | 547515.8 | 625570.7 |
3.6.6 precision test
Mix reference substance solution 10ul under getting 3.6.3 item, according to chromatographic condition continuous sample introduction 6 pin under 3.6.2 item, obtain peak area, and calculate the RSD value of peak area.The results are shown in Table 16.
Table 16 mixes reference substance precision test
Result shows that its RSD value is all less than 2%, therefore instrument precision is good.
3.6.7 stability test
Mix reference substance and Cortex Magnoliae Officinalis test sample 10ul under getting 3.6.3 item, according to chromatographic condition under 3.6.2 item respectively at 0,2,4,8,12,24h sample introduction.Obtain peak area, and calculate the RSD value of peak area, the results are shown in Table 17,18.
Table 17 mixes reference substance stability test
Table 18 Cortex Magnoliae Officinalis test sample stability test
From result, its RSD is all less than 2%, mixing reference substance and need testing solution basicly stable in 24h.
3.6.8 replica test
According to the parallel sample preparation of the preparation of Cortex Magnoliae Officinalis test sample 6 parts under 3.6.3 item, get 10ul for every part, according to chromatographic condition sample introduction under 3.6.2 item.Obtain peak area, and calculate the RSD value of peak area.The results are shown in Table 19.
Table 19 Cortex Magnoliae Officinalis test sample replica test
Result shows, and RSD value is all less than 3%, illustrates that the repeatability of method for making sample is good.
3.6.9 average recovery test
Parallel precision measures the Cortex Magnoliae Officinalis decocting liquid 2ml identical with 6 parts of assays, and it is appropriate to add magnolol and honokiol reference substance, according to test sample preparation method preparation under 3.6.3 item.Get 10ul according to chromatographic condition sample introduction under 3.6.2 item for every part.Calculate the response rate and RSD value, acquired results is as shown in following table 20,21.
Table 20 honokiol average recovery is tested
Table 21 magnolol average recovery is tested
Result shows, and average recovery is within the scope of 95%-105%, and RSD is all less than 2%.Prompting sample preparation methods is feasible.
3.6.10 the honokiol of Cortex Magnoliae Officinalis and Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) and Determination of Magnolol measure
According to the preparation method of 3.6.3 sample, parallel preparation 3 parts of Cortex Magnoliae Officinalis 10 minutes medicinal liquids and processed product 5 test sample, according to 3.6.2 chromatographic condition, sample introduction 10ul, the parallel sample introduction of each sample 3 times.Measure average content and the RSD value of honokiol in medicinal liquid and magnolol.Acquired results is as shown in table 22,23, and the chromatogram of magnolol 10 minutes medicinal liquids and processed product 5 phenolic constituent as shown in Figure 5,6.
Table 22 honokiol assay result
Table 23 Determination of Magnolol measurement result
From result, RSD is all in 3%.Compared with processed product 5 and Cortex Magnoliae Officinalis 10 minutes medicinal liquids, honokiol and Determination of Magnolol slightly reduce.
The tenuifolin assay of 3.7 Radix Polygalaes and Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed)
3.7.1 experiment material and instrument
Tenuifolin (purity is greater than 98.0%, Man Site bio tech ltd, Chengdu), chromatograph methanol are chromatographically pure, and other reagent such as methanol, pure water, phosphoric acid are analytical pure.
Shimadzu LC-20AT high performance liquid chromatograph (SHIAZDU company), UV detector, CP124S electronic analytical balance (SARTORIUS).
3.7.2 chromatographic condition and system suitability test
Boston Green ODS C 18 chromatographic column (4.6 × 250mm, 5 μm,
); Mobile phase methanol-0.05% phosphoric acid solution (55:45) is mobile phase; Flow velocity 1mlmin
-1; Column temperature: 30 DEG C; Determined wavelength is 210nm.Separating degree is greater than 1.5, and theoretical cam curve calculates by tenuifolin peak and is not less than 3000.
3.7.3 the preparation of sample
The preparation of tenuifolin reference substance solution gets tenuifolin reference substance in right amount, accurately weighed, adds methanol and makes the solution of every 1ml containing 1mg, to obtain final product.Gained chromatogram as shown in Figure 7.
Cortex Magnoliae Officinalis through leaving standstill 10 minutes medicinal liquids, Radix Polygalae 20 minutes medicinal liquids, each supernatant of processed product 5 are drawn in the preparation of need testing solution, after the centrifugal 10min of 3000rmp, precision measures each centrifugal rear medicinal liquid 5ml, add 11% sodium hydroxide solution 50ml, be mixed with 10% sodium hydroxide solution 55ml, reflux 2 hours, lets cool, and is 4 ~ 5 with salt acid for adjusting pH value, 3 times are extracted with water saturated n-butyl alcohol jolting, each 50ml, merges n-butyl alcohol liquid, and recycling design is to dry, residue adds methanol makes dissolving in right amount, be transferred in 25ml measuring bottle, add methanol to scale, shake up.Cross 0.45um microporous filter membrane, to obtain final product.
3.7.4 negative interference test
Get Radix Polygalae test sample 10ul under 3.7.3 item, according to chromatographic condition sample introduction under 3.7.2 item, detect, do not find interference.Result illustrates that the mensuration of Cortex Magnoliae Officinalis single medicinal liquid to Radix Polygalae medicine liquid ingredient is not disturbed, and refers to Fig. 8.
3.7.5 linear relationship is investigated
Get tenuifolin reference substance 2ul, 4ul, 6ul, 8ul, 10ul, 12ul, 16ul sample introduction under 3.7.3 item respectively, obtain the relation of its quality and peak area, calculate regression equation and R
2value, the R of tenuifolin
2value is 0.9996, illustrates that linear relationship is good, the results are shown in following table 24 and Fig. 9.
The quality of table 24 tenuifolin and peak area relation
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| M(ug） | 1 | 2 | 3 | 4 | 5 | 6 | 8 |
| A | 172783.4 | 341972.8 | 523809.3 | 698078.8 | 874684.4 | 1056384.0 | 1377819.3 |
3.7.6 precision test
Get tenuifolin reference substance solution 10ul under 3.7.3 item, according to chromatographic condition continuous sample introduction 6 pin under 3.7.2 item, obtain peak area, and calculate the RSD value of peak area.The results are shown in Table 25.
Table 25 tenuifolin is according to product precision test
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | RSD（%） |
| Tenuifolin | 874684.4 | 888647.7 | 869054.6 | 887554.2 | 899902.0 | 885456.1 | 1.24 |
Result shows that its RSD value is all less than 2%, therefore instrument precision is good.
3.7.7 stability test
Get tenuifolin reference substance and Radix Polygalae test sample 10ul under 3.7.3 item, according to chromatographic condition under 3.7.2 item respectively at 0,2,4,8,12,24h sample introduction.Obtain peak area, and calculate the RSD value of peak area, the results are shown in Table 26,27.
Table 26 tenuifolin reference substance stability test
| Time (h) | 0 | 2 | 4 | 8 | 12 | 24 | RSD（%） |
| Tenuifolin | 874684.4 | 903083.7 | 871134.8 | 885089.9 | 875222.3 | 879744.8 | 1.32 |
Table 27 Radix Polygalae test sample stability test
| Time (h) | 0 | 2 | 4 | 8 | 12 | 24 | RSD（%） |
| Tenuifolin | 471822.2 | 464063.7 | 471072.4 | 466371.6 | 472512.8 | 466072.6 | 0.76 |
From result, its RSD is all less than 2%, and tenuifolin reference substance and need testing solution are basicly stable in 24h.
3.7.8 replica test
According to the parallel sample preparation of the preparation of Radix Polygalae test sample 6 parts under 3.7.3 item, get 10ul for every part, according to chromatographic condition sample introduction under 3.7.2 item.Obtain peak area, and calculate the RSD value of peak area.The results are shown in Table 28.
Table 28 Radix Polygalae test sample replica test
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | RSD（%） |
| Tenuifolin | 446933.7 | 481060.1 | 471822.2 | 470000.2 | 467599.0 | 472454.0 | 2.44 |
Result shows, and RSD value is all less than 3%, and the repeatability of method for making sample is good.
3.7.9 average recovery test
Parallel precision measures the Radix Polygalae water decoction 5ml identical with 6 parts of assays, adds tenuifolin reference substance in right amount, according to test sample preparation method preparation under 3.7.3 item.Get 10ul according to chromatographic condition sample introduction under 3.7.2 item for every part.Calculate the response rate and RSD value, acquired results is as shown in following table 29.
Table 29 tenuifolin average recovery is tested
Result shows, and average recovery is within the scope of 95%-105%, and RSD is less than 3%.The preparation method of prompting sample is feasible.
3.7.10 the assay result of tenuifolin in each medicinal liquid
According to the preparation method of 3.7.3 sample, parallel preparation 3 parts of Radix Polygalaes 20 minutes medicinal liquids and processed product 5 test sample, according to 3.7.2 chromatographic condition, sample introduction 10ul, the parallel sample introduction of each sample 3 times.Measure average content and the RSD value of the tenuifolin in medicinal liquid.Acquired results is shown in table 30, and the chromatogram of Radix Polygalae 20 minutes medicinal liquids and processed product 5 tenuifolin is as shown in Figure 10,11.
Table 30 tenuifolin assay result
Result shows, and RSD is all in 3%.Compared with processed product 5 and Radix Polygalae 20 minutes medicinal liquids, tenuifolin content slightly raises.
By the process of preparing Chinese medicine of orthogonal trial difference, decocting process, Stomach residue rate, small intestinal charcoal end Intestinal pushing is adopted to test the processing procedure that preferably can reduce Radix Polygalae digestive tract power and suppress side effect; Adopt pharmacological experiment, investigate the calmness of each processed product, antitussive action to verify its drug effect; Adopt HPLC method, the changes of contents of comparative measurements Radix Polygalae, Cortex Magnoliae Officinalis and preferred processed product thereof.
Result: 1. in each processed product, processed product 5 compares with the single Radix Polygalae group decocting 20min, 60min, significantly can reduce the Stomach residue rate of mice and increase alvine pushing rate (P < 0.05).2. the processed product optimized 5 groups can strengthen pentobarbital sodium and works in coordination with sedation (P < 0.01) in pole significantly, its each dosage group significantly can also reduce the autonomic activities rate (P < 0.01, P < 0.05) of mice; And the cough caused cough latent period of mice ammonia (P < 0.01) can be extended significantly in pole, reduce cough number of times (P < 0.01), its 30g/kg group significantly can increase the phenol red absorbance of tracheal strips of mice and concentration (P < 0.05) and have phlegm-dispelling functions.3. single Cortex Magnoliae Officinalis decocts that the honokiol of 10min group and magnolol average content are respectively 0.100,0.153mg/g, and processed product 5 is respectively 0.073,0.111mg/g; And the tenuifolin content that 20min group decocted by Radix Polygalae list is 2.650mg/g, processed product 5 is 3.840mg/g.
Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product of the present invention is finally selected to adopt following methods preparation:
1. Cortex Magnoliae Officinalis juice preparation: get magnolia medicament 70-130g, 8-12 times amount water soaking 15-45min, intense fire boils rear slow fire boiling 5-15min, is evaporated to 10-40ml and is Cortex Magnoliae Officinalis concentrated juice.
2. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get Polygala tenuifolia 30-70g, add 10-40ml Cortex Magnoliae Officinalis concentrated juice moistening 1-5h, 6-18min is processed in 60-180 DEG C of stir-fry, is drying to obtain.
Processed product 5, namely the preferred preparation technology of Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product of the present invention is as follows:
1. Cortex Magnoliae Officinalis juice preparation: get magnolia medicament 100g, 8-12 times amount water soaking 30min, intense fire boils rear slow fire boiling 10min, is evaporated to 25ml and is Cortex Magnoliae Officinalis concentrated juice.
2. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get Polygala tenuifolia 50g, add 25ml Cortex Magnoliae Officinalis concentrated juice moistening 3h, 12min is processed in 120 DEG C of stir-frys, is drying to obtain.
Processed product decocting condition of the present invention: get the Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) (theoretical crude drug amount 150g) that method is obtained, add 8-12 times of water soaking 15-45min, intense fire boils rear slow fire boiling 10-30min, concentrating under reduced pressure, be settled to 100ml, be equivalent to containing primary crude drug total amount 1.5g/ml.
Preferred decocting condition is: get the Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed) (theoretical crude drug amount 150g) that method is obtained, add 10 times of water soaking 30min, intense fire boils rear slow fire boiling 20min, and concentrating under reduced pressure, is settled to 100ml, is equivalent to containing primary crude drug total amount 1.5g/ml.
The Radix Polygalae prepared of above-mentioned condition concocts decoction pieces, and the digestive tract power that effectively can reduce crude Radix Polygalae suppresses side effect, and antitussive can be retained, eliminate the phlegm, drug effect of calming the nerves, reach and subtract the object that pair deposits effect, there is clinical value.
Claims (4)
1. Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product, is characterized in that: Radix Polygalae is fried after being soaked by Cortex Magnoliae Officinalis juice and processed and get final product;
Wherein, preparation method comprises the steps:
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 1 part, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 5min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 10ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 6min 60 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 1 part, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 10min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 25ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 9min 90 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 5min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 10ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 9min 90 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 10min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 25ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 12min 120 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 15min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 40ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 6min 60 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 1 part, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 15min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 25ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 6min 90 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 5min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 25ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 6min 120 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 10min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 40ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 9min 60 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 3 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 5min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 40ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 12min 90 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product.
2. the preparation method of Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product according to claim 1, is characterized in that: it comprises the steps:
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 1 part, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 5min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 10ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 6min 60 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 1 part, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 10min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 25ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 9min 90 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 5min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 10ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 9min 90 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 10min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 25ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 12min 120 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 15min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 40ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 6min 60 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 1 part, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 15min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 25ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 6min 90 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 5min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 25ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 6min 120 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 2 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 10min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 40ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 9min 60 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product;
Or
A. Cortex Magnoliae Officinalis with Radix Polygalae by crude drug ratio is: Cortex Magnoliae Officinalis 3 parts, Radix Polygalae 1 part;
B. Cortex Magnoliae Officinalis juice is prepared: get magnolia medicament and decoct with water, decocting time is 5min, filters to obtain Cortex Magnoliae Officinalis juice; By Radix Polygalae crude drug 50g, step B, the Cortex Magnoliae Officinalis juice after filtration is condensed into 40ml;
C. Cortex Magnoliae Officinalis juice Cortex et Radix Polygalae (processed): get step B gained Cortex Magnoliae Officinalis concentrated juice and soak Radix Polygalae, absorb Cortex Magnoliae Officinalis concentrated juice to Radix Polygalae, process 12min 90 DEG C of stir-frys, after due-in juice, be drying to obtain Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product.
3. the preparation method of Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product according to claim 2, is characterized in that: the time that in step B, Cortex Magnoliae Officinalis is soaked before decocting is 15-45min.
4. the preparation method of Cortex Magnoliae Officinalis Cortex et Radix Polygalae (processed) processed product according to claim 3, is characterized in that: the time that in step B, Cortex Magnoliae Officinalis is soaked before decocting is 30min.
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