CN102836653B - Liquid drop mixing unit based on electro-wetting digital micro-fluid chip - Google Patents
Liquid drop mixing unit based on electro-wetting digital micro-fluid chip Download PDFInfo
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- CN102836653B CN102836653B CN201210351244.5A CN201210351244A CN102836653B CN 102836653 B CN102836653 B CN 102836653B CN 201210351244 A CN201210351244 A CN 201210351244A CN 102836653 B CN102836653 B CN 102836653B
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Abstract
The invention belongs to the technical field of digital micro-fluidics, and in particular relates to a liquid drop mixing unit based on an electro-wetting digital micro-fluid chip. The mixing unit comprises three parts consisting of an inlet zone, a mixing zone and an outlet zone; the fast, high-efficiency, automatic and reliable mixing of the liquid drops can be realized via the special bidirectional driving electrode and closed-loop electrode arrangement and simplified electrode connection mode. The liquid drop mixing unit has the advantages of novel design, easiness in control, high reliability, excellent practicability, high automation degree, and the like, so that the liquid drop mixing unit can be widely used in various digital micro-fluid chips.
Description
Technical field
The invention belongs to digital microfluidic technology field, be specifically related to based on the medium wetting digital microfluidic technology chip that powers on, relate in particular to a kind of droplets mixing unit based on the wetting digital micro-fluid chip of electricity.
Background technology
Chip lab (Lab-on-a-chip, LOC), as an emerging technology, owing to having many advantages such as high integration, high accuracy, low consumption, is just receiving increasing concern, has good research and application prospect.And so-called chip lab refers to that can complete biochemistry processes each process, can automatically complete the sophisticated functions microminiaturization of traditional experiment chamber task, integrated MEMS system.Its target is integrated analytic process completely on individual devices, can complete the behaviour such as sample extraction, sample pretreatment, decomposition and separation, biochemical reaction, analyzing and testing, data processing and do.As the power section of chip lab, microflow control technique plays vital effect.And refer to based on the wetting digital microfluidic technology of electricity a microfluidic technology controlling single drop by applying voltage, owing to thering is relatively simple, good controllability and driving force, be considered to the most feasible, most with prospects technology in LOC application.
At present based on the wetting digital microfluidic technology of electricity the function on practical chip mainly concentrate on distribution to fluid sample, transport, mix, the multiple processing such as extraction, separation, and this function of the mixing of sample is most important, being the foundation of digital micro flow chip application, is the key of chip lab microminiaturization, automation, commercialization.
In the wetting digital micro flow chip of current electricity, the large class methods of mixing main two of sample: the first kind is simple directly mixing on chip,, by driving two kinds of sample drop to merge, makes thereafter to merge drop and transports stretch footpath, in transport process, two kinds of samples are realized slowly and being mixed.This method is exactly to utilize droplet coalescence and the transportation function of digital micro flow chip in fact, can directly utilize the drive electrode of chip, and method is directly simple, does not need additional functionality unit.But also there is distinct disadvantage in this method, first be that mixing rate is very slow, because it is to rely on the drop transport process of traditional die to complete, and traditional drop transports the path that can only take the air line, the mixing efficiency of drop is very low like this, drop transport path that need to be longer or longer incorporation time.Secondly, because the mixing of drop does not have special functional unit, and the mixing of drop long period transports the pollution that can cause chip.Finally, owing to being subject to the constraint of the common drive electrode of chip, to mix the size dimension of drop restricted.And Equations of The Second Kind mixed method is to adopt extra householder method, for example on digital micro flow chip, the method such as integrated sonication, magnetic agitation is mixed, this class mixed method can obtain good mixing efficiency and effect, but need extra functional unit, increase complexity and the commercial cost of chip, and the low microminiaturization that limits micro flow chip of integrated level.
Therefore, need a kind of simplely, good mixing effect, mixing efficiency is high, integrated level is high, cost is low droplets mixing functional device are to be integrated in digital micro flow chip, to meet the application requirements of chip lab.
Summary of the invention
The present invention aims to provide a kind of droplets mixing unit based on the wetting digital micro flow chip of electricity, to be integrated in digital micro flow chip, can realize drop simply, efficiently mix.
Mixing principle of the present invention is based on the transporting of drop, by drop is transported to certain path in a certain way fast to reach the object of automatic mixing.
Droplets mixing unit based on the wetting digital micro flow chip of electricity provided by the invention, comprises three parts:
Part I is the inlet region of mixed cell; This part is mainly that traction drop enters mixed cell, the drive electrode of intake section can be absolute electrode, also can be a part for mixed cell internal drive electrode electrode, the shape size of electrode does not limit, and outer mixed cell drop can be transported to the combination drive electrode in mixed cell as long as meet.Position and the number of entrance also do not limit.
Part II is the mixed zone of mixed cell; This part is the main functional parts of mixed cell, implements the efficient mixing to drop.This part is made up of unidirectional drive electrode.Each drive electrode head and the tail lean on mutually, the circulus of mutually nested formation sealing, and the mixed path that makes drop is annulus curve.Utilize this design, make drop no matter in the motion of single electrode or in whole piece mixed path, move and be curve, can improve widely like this mixing efficiency of drop; And this part uses and simplifies electrode and be connected, alternate electrode is electrically connected, make so the minimum control signal of whole mixed cell can be two, greatly simplify control system.In this part, the drive electrode of mixed cell is at least 4, but and no maximum, as long as can ensure that electrode is arranged into annular enclosed structure.
The unidirectional drive electrode of this part can adopt following frame mode.Electrode shape is asymmetric discrete electrodes, each electrode is divided into 3 parts: the one, be the electrode head that corynebacterium and top are circular arc (E41), this part is used for drawing adjacent electrode drop to this electrode in the time starting, and top is circular arc, to increase drop driving force; The 2nd, with the electrode middle part (E42) of round breach, this part is the excessive part that drop starts to contact drive electrode and be stabilized in to drop drive electrode, and the effect of circumferential notch is increase drop driving force and one-way; The 3rd, the circular-arc and afterbody electrode afterbody jaggy (E43) of square, this part is the steady component of drop on drive electrode, afterbody breach be convenient to nested with the head of adjacent electrode; The electrode structure of entirety meets following condition: for a certain size drop, and no matter its any position on electrode, as long as an effective coated electrode part applies driving signal to electrode, it can finally arrive the steady component of electrode afterbody;
Part III is the outlet area of mixed cell; This part is mainly by the drop output of mixed cell, the drive electrode of exit portion can be absolute electrode, also can be a part for mixed cell internal drive electrode electrode, the shape size of electrode does not limit, thereby but will meet drop in this part and can effectively touch combination drive electrode and make drop can input mixed cell to mix.Position and the number of outlet also do not limit.
The mixed cell that it should be pointed out that traditional digital micro flow chip does not clearly define entrance, outlet, and definition of the present invention is conducive to drop and passes in and out fast mixed cell, and can with the effective combination of other functional unit of micro flow chip.This is one of innovative point of the present invention.
And in the present invention, the transport path while utilizing the circular path of asymmetric drive electrode and sealing to make droplets mixing is not straight line, can greatly improve like this mixing efficiency, this is again innovative point of the present invention.
In addition, in the present invention, owing to using unidirectional drive electrode and simplify electrode connection mode, do not need to know in advance droplet position, only need apply in turn drive that signal can realize drop High-Speed Automatic to two drive electrodes and transport mixing.
In the present invention, described " unit " refers to the organic assembling of multiple parts with certain function.
In the present invention, described " drop " refers to that can be used for the wetting solution driving of electricity drips, its composition can be single biological sample, chemical solution etc., also can be multicomponent composition, drop of one deck oil film etc. as coated outside, its size does not limit, and can slightly be raised between some milliliters for inferior.
In the present invention, described " drive electrode " refers to that the voltage of counter electrode when chip is implemented is set to and is not that 0 so that the wetting driving of electricity can occur, and described " earth electrode " refers to that the voltage of counter electrode when chip is implemented is set to 0 or enough approach with 0.
In the present invention, described " mutually nested " refers to some graph-inclusion district between drive electrode, but is not connected on electric, and while touching certain part of a certain drive electrode with bonding drop, it also can contact a part for corresponding adjacent electrode.
Droplets mixing provided by the invention unit has following significant advantage:
1. directly utilize drop transportation function, simple and convenient, be easy to integrated;
2. there is import and export part, can realize drop and pass in and out fast, and can with the effective combination of other parts;
3. compared with conventional hybrid method, there is mixing rate and better mixed effect faster;
4. mixing portion only needs two to drive signal, greatly reduces control complexity;
5. utilize unique unidirectional electrode, can realize full-automatic mixing, there is higher reliability;
6. utilize unique electrode configuration, can make combination drive electrode number increase arbitrarily the control that does not but affect chip.
Brief description of the drawings
Fig. 1 is the wetting digital micro-fluid chip structural representation of one electricity of droplets mixing of the present invention unit.
Fig. 2 is the droplets mixing cell schematics based on the wetting digital micro-fluid chip of electricity of the present invention.
Fig. 3 is a kind of unidirectional drive electrode structure diagram of the present invention.
Detailed description of the invention
Can there be multiple concrete chip implementation droplets mixing unit based on the wetting digital micro-fluid chip of electricity provided by the invention, and present embodiment is only enumerated a kind of common digit chip structure and implemented principle so that it to be described.It should be pointed out that present embodiment is to provide for illustration purpose, and pay no attention to limiting the scope of the invention by any way.
Based on the wetting digital micro-fluid chip structure of a kind of electricity of the present invention as shown in Figure 1.On substrate 11, be mixed cell drive electrode 1-4 of the present invention.Fixing as the material of substrate, as long as insulation, as being the silicon chip etc. of quartz, glass, insulation; And electrode (comprising earth electrode described below) can be made up of any conductive material.Should illustrate, the combination drive electrode of schematic diagram is only taking 4 as example, but its actual number do not limit, and the just simple position of electrode in chip that represent of drive electrode in figure, do not reflect in detail that it specifically arranges and connected mode, and be not described in detail chip structure.Electrode 1-4 is upper for dielectric layer 12, is hydrophobic layer 13 on it.It should be pointed out that dielectric layer should be dielectric material but do not limit, and is preferably the material that dielectric constant is higher, breakdown characteristics is stronger.Substrate 11, drive electrode 1-4, dielectric layer 12, hydrophobic layer 13 have formed the bottom crown 1 of chip jointly.On bottom crown, be driving drop D; On drop, be hydrophobic layer 23, medium 22, and basic 21, they have formed the top crown 2 of chip jointly.The structure of whole chip is that drop is clipped between bottom crown, and when bottom crown drive electrode, 1-4 applies voltage signal on request, and when top crown earth electrode ground connection, and drop will corresponding generation drives and transports, thereby realizes high-speed mixing.
Fig. 2 is the droplets mixing cell schematics based on the wetting digital micro-fluid chip of electricity of the present invention.Mixed cell forms nested closed-loop structure by 4 drive electrode 1-4, realizes mixing in order to transport drop; Mixed cell comprises an entrance 5, should be understood that, the form of inlet electrode 5 does not limit, can be for independently electrode and electrode shape basis specifically change, this explanation only configures in the mode of simplifying, by the inlet electrode of a certain shape 5 with transport drive electrode 1 and be connected, to realize co-controlling, the function that does not affect mixed cell realizes.In addition, mixed cell also comprises an outlet 6, and the form of outlet does not also limit, and the present invention is caved in as outlet using the part of electrode 2, in order to be connected to facilitate the mixed drop of output with outer electrode.Whole mixed cell adopts the mode of simplifying to connect, and finally draws two and drives signal connecting electrode A, B.In the time that mixed cell adopts the chip structure configuration of Fig. 1, if drop is positioned at driver element porch, as D1, by connecting electrode A time voltage is driven to signal, chip top crown ground connection simultaneously, drop will enter mixed cell so, final in mixed cell a certain mixing transport the stable place of electrode, as D2.Thereafter, if keep chip top crown ground connection, by connecting electrode B time voltage is driven to signal, and A cancels driving signal, and drop will also finally be stabilized in toward electrode 2 motions the stable place of electrode 2, as D3.In turn A, B connecting electrode are applied to driving signal with this, drop will do annular at electrode 1,2,3,4 with this and transport, thereby realizes droplets mixing.If in the time that drop is positioned at the stable place of electrode 2, as D3, cancel the driving signal of electrode A, B simultaneously, drop will keep motionless always, now drop can be exported to mixed cell by exporting 6.In this embodiment, whole mixed cell can be realized input, mixing, the output function of drop fast, has greater efficiency and better effects.
Claims (2)
1. the droplets mixing unit based on the wetting digital micro-fluid chip of electricity, is characterized in that being made up of inlet region, mixed zone and outlet area three parts, adopts the quick transfer ways of drop to realize and mixes; Wherein:
Inlet region is used for drawing drop and enters mixed cell, and the drive electrode of inlet region part is absolute electrode, or a part for mixed cell internal drive electrode;
Mixed zone is main functional parts, implements the mixing to drop; Mixed zone is made up of unidirectional drive electrode, and each drive electrode head and the tail lean on mutually, the circulus of mutually nested formation sealing, and the mixed path that makes drop is annulus curve;
Outlet area is for exporting the drop of mixed cell, and the drive electrode of outlet area part is absolute electrode, or a part for mixed cell internal drive electrode;
The unidirectional drive electrode of described mixed zone adopts following frame mode: electrode shape is asymmetric discrete electrodes, each electrode is divided into 3 parts: the one, be the electrode head that corynebacterium and top are circular arc (E41), this part is used for drawing adjacent electrode drop to this electrode in the time starting, top is circular arc, to increase drop driving force; The 2nd, with the electrode middle part (E42) of round breach, this part is the transition portion that drop starts to contact drive electrode and be stabilized in to drop drive electrode, and the effect of circumferential notch is increase drop driving force and one-way; The 3rd, the circular-arc and afterbody electrode afterbody jaggy (E43) of square, this part is the steady component of drop on drive electrode, afterbody breach be convenient to nested with the head of adjacent electrode; The electrode structure of entirety meets following condition: for a certain size drop, and no matter its any position on electrode, as long as an effective coated electrode part applies driving signal to electrode, it can finally arrive the steady component of electrode afterbody.
2. the application of droplets mixing unit in the digital micro flow chip that unipolar plate drives, bipolar plates drives various structures as claimed in claim 1.
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| CN201210351244.5A CN102836653B (en) | 2012-09-20 | 2012-09-20 | Liquid drop mixing unit based on electro-wetting digital micro-fluid chip |
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| CN201210351244.5A CN102836653B (en) | 2012-09-20 | 2012-09-20 | Liquid drop mixing unit based on electro-wetting digital micro-fluid chip |
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| CN102836653B true CN102836653B (en) | 2014-08-06 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103143406B (en) * | 2013-03-10 | 2015-01-07 | 复旦大学 | Two-dimensional digital micro-fluidic chip based on one-way liquid drop transport |
| CN104248997B (en) * | 2013-06-27 | 2016-07-27 | 李木 | A kind of digital microfluidic chip and control method thereof |
| CN103412024B (en) * | 2013-07-05 | 2016-01-20 | 复旦大学 | A kind of integrated electrochemical sensing chip |
| US10464067B2 (en) | 2015-06-05 | 2019-11-05 | Miroculus Inc. | Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling |
| EP3303548A4 (en) | 2015-06-05 | 2019-01-02 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
| CN105854117B (en) * | 2016-03-28 | 2022-03-15 | 南京理工大学 | Accurate infusion set that doses based on electrowetting on medium |
| US10596572B2 (en) | 2016-08-22 | 2020-03-24 | Miroculus Inc. | Feedback system for parallel droplet control in a digital microfluidic device |
| CA3049416A1 (en) | 2016-12-28 | 2018-07-05 | Miroculus Inc. | Digital microfluidic devices and methods |
| WO2018187476A1 (en) | 2017-04-04 | 2018-10-11 | Miroculus Inc. | Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets |
| CN110892258A (en) | 2017-07-24 | 2020-03-17 | 米罗库鲁斯公司 | Digital microfluidic system and method with integrated plasma collection device |
| US11738345B2 (en) | 2019-04-08 | 2023-08-29 | Miroculus Inc. | Multi-cartridge digital microfluidics apparatuses and methods of use |
| US11524298B2 (en) | 2019-07-25 | 2022-12-13 | Miroculus Inc. | Digital microfluidics devices and methods of use thereof |
| US11772093B2 (en) | 2022-01-12 | 2023-10-03 | Miroculus Inc. | Methods of mechanical microfluidic manipulation |
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| CN1588090A (en) * | 2004-08-27 | 2005-03-02 | 清华大学 | Low voltage micro liquid drop control device |
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| US6911132B2 (en) * | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
| JP2010081838A (en) * | 2008-09-30 | 2010-04-15 | Nsk Ltd | Microfluidic chip and cell counter |
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| CN1588090A (en) * | 2004-08-27 | 2005-03-02 | 清华大学 | Low voltage micro liquid drop control device |
| CN101294971A (en) * | 2008-06-05 | 2008-10-29 | 复旦大学 | Digital microcurrent-controlled device and control method based on electrowetting effect on dielectric |
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