CN102816683B - Biochemical culture and detection device as well as detection method thereof - Google Patents
Biochemical culture and detection device as well as detection method thereof Download PDFInfo
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- CN102816683B CN102816683B CN 201210269999 CN201210269999A CN102816683B CN 102816683 B CN102816683 B CN 102816683B CN 201210269999 CN201210269999 CN 201210269999 CN 201210269999 A CN201210269999 A CN 201210269999A CN 102816683 B CN102816683 B CN 102816683B
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- pilot trench
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
Abstract
The invention relates to a biochemical culture and detection device as well as a detection method of the device, belonging to the technical field of microorganism culture. The biochemical culture and detection device comprises a bottom plate (1) and a top cover (3), wherein the bottom plate (1) comprises a culture pond (11) and a receiving region (12); bumps (122) in the receiving region are radially arranged around the culture pond (11) and used for dividing the receiving region (12) into multiple fan-shaped storage locations; culture sub-grooves (2) are like hollow wedge-shaped bodies and are arranged in the storage locations in the receiving region (12); the culture sub-grooves (2) adopt fully-opened culture sub-grooves (2a) or partially-opened culture sub-grooves (2b); the culture pond (11) is used for receiving liquid culture mediums to be detected; and the culture sub-grooves (2) are used for receiving detected culture mediums. The incubating process is finished by rocking the biochemical culture and detection device. The functions of enriched culture, coated incubation and biochemical identification are integrated, the operation is flexible, the requirement on operating environment and the detection cost are lowered, and the detection efficiency and the stability of results are improved.
Description
Technical field
The present invention relates to a kind of microbial biochemical cultivation and proofing unit and detection method thereof, belong to microorganism culturing technique with the apparatus field.
Background technology
At present, in biomedicine detected and studies, Bacteria Detection was occupied significant proportion.How fast identify the state, bacterium drug susceptibility of biology kind, the heritable variation of contained bacterium in the sample etc. exactly, become Interventions Requested indispensable in the routine work.
The API biochemistry detection test kit of Xiao Shouing is with complex operation, tediously long traditional method traceization and the stdn of explanation in the market; With the result of biochemical reaction result in conjunction with other methods of inspection, in through statistical study data processed storehouse, compare, and then make kind and differentiate.But also there is following defective in API biochemistry detection test kit:
1, API biochemistry detection test kit can not be realized increasing integrated that bacterium is cultivated and biochemistry is differentiated, need increase bacterium earlier and cultivate, and then carry out a series of biochemical investigation in containing the tubule of reagent.Owing to cultivate the conversion of vessel and the design limitation of containing the plastics tubing of reagent, need under the higher environment of aseptic condition, just can operate, otherwise may be caused the influence to assay by the bacterial contamination in the external world.
2, it is less that biochemical reaction is tested little pipe volume, gives in the operation and make troubles.
3, utilize API biochemistry detection test kit to carry out Bacteria Detection before, need increase bacterium-separation and Culture, obtain single bacterium colony.And the substratum that contains certain nutritive ingredient only is fit to the growth of some specific bacteria; If the contained bacterium of sample to be checked is unknown, then must adopt a plurality of culture dish, take up the substratum trial property test that contains the Different Nutrition composition respectively, to satisfy the needs of separation of bacterial; In addition, also need the bacterium of gained list bacterium colony is carried out some special checks, as gram stain microscopy, oxidase test etc.; When these are to use the API test kit the precondition that must satisfy, increased complicacy and the fussy degree of using the API system on the whole, and whole flow process is consuming time longer.
4, API biochemistry detection test kit still adopts manual inoculation, and efficient is low, is not suitable for the mass-producing operation.
5, use the cost of API biochemistry detection test kit higher.
Summary of the invention
The objective of the invention is to overcome the deficiency of above-mentioned biochemistry detection test; provide a kind of energy will increase bacterium cultivation, inoculation, biochemical integrated device and the detection method thereof differentiated; this installs, and inoculation, cultivation and detection method are simple, flexible operation; safe and reliable; improved the stability of detection efficiency and detected result; reduced requirements for operation circumstances and detection cost, be fit to mass-producing and use.
The object of the present invention is achieved like this:A kind of biochemical cultivation and proofing unit comprises the chassis and the top cover that are connected, and described chassis comprises and be arranged at the recessed cultivation pool of chassis central authorities and be centered around annular disposal area around the cultivation pool,
At the bottom of described disposal area comprises the inclination of tilting to cultivation pool, be arranged on several strip projected parts, internal perisporium and the periphery wall of end upper surface, turn up and be connected with the upper end of the internal perisporium of disposal area in the upper edge of described cultivation pool, described projection is radial arrangement around cultivation pool, and the disposal area is separated into several fan-shaped warehouse compartments;
Described chassis is provided with several and cultivates pilot trench, described cultivation pilot trench is hollow sphenoid, cultivate the narrow end of pilot trench towards cultivation pool, be shelved in the warehouse compartment of disposal area, described cultivation pilot trench is that full open model is cultivated pilot trench or the open cultivation pilot trench of part, described full open model is cultivated the top of pilot trench and is not established cover plate, the top of the open cultivation pilot trench of described part is provided with a cover plate, described cover plate is trapezoidal, the length of described cover plate is less than the notch length of cultivating pilot trench, described cover plate is arranged on the narrow end of cultivating pilot trench, and is closed fixing with the upper edge of cultivating pilot trench.
The cross section on described chassis is circular or oval.
The upper edge of described cultivation pool and the angle of horizontal plane are α, and the scope of α is that 0 degree is to 90 degree.
The upper edge of described cultivation pool and the angle of horizontal plane are α, and the scope of α is that 20 degree are to 45 degree.
The number of the warehouse compartment of described disposal area is 3～30.
Twice rib places, the outside of described cultivation pilot trench respectively are provided with half groove, and half of described groove is connected with half of projection.
Angle with horizontal plane at the bottom of the inclination of described disposal area is β, and the scope of β is that 0 degree is to 90 degree.
The lower ends downward extension of the periphery wall of described disposal area flushes with the bottom of cultivation pool.
The detection method of a kind of biochemical cultivation and proofing unit may further comprise the steps:
Step 1: preparation liquid, solid, semisolid medium etc. detect substratum;
Step 2: the cultivation pilot trench of above-mentioned detection substratum being inserted test design scheme correspondence;
Step 3: be placed on above-mentioned cultivation pilot trench on the warehouse compartment on apparatus of the present invention chassis successively by the test design scheme;
Step 4: in cultivation pool, inject liquid nutrient medium;
Step 5: sample to be detected is seeded in the aforesaid liquid substratum;
Step 6: at a certain temperature, leave standstill and cultivate for some time, or low speed wave and culture for some time, shake speed and do not spill cultivation pool with liquid nutrient medium and be as the criterion;
Step 7: said apparatus is shaken the coating inoculation, the liquid nutrient medium in the cultivation pool is got rid of into the cultivation pilot trench, the speed of shaking does not spill apparatus of the present invention with liquid nutrient medium and is as the criterion;
Step 8: add the required reagent of test or test paper;
Step 9: cultivation or low speed wave and culture are left standstill in the said apparatus placement at a certain temperature;
Step 10: with the naked eye or instrument direct viewing and detect in the above-mentioned cultivation pilot trench bacterium colony, cell colony or organize the colony kind and muddy degree, the colour-change of form or mixed solution, or add detection reagent and further observe and detect.
In step 6, step 7, step 9, described shaking by hand-held this device shaken, and maybe this device placed on the shaking table equipment and shakes.
The invention has the beneficial effects as follows:
A kind of biochemical cultivation of the present invention and proofing unit and detection method thereof characteristics as follows:
(1) simultaneously receiving fluids, solid, semisolid medium, be about to various functions substratum such as enrichment medium, select substratum, differential medium to concentrate in together;
(2) various substratum are separated from each other, and do not disturb mutually, and can be in contact with one another under the certain physical condition, avoid the substratum of different phases to mix mutually detected result is caused interference, make detected result reliable and stable;
(3) bacterium is in the inner inoculation of device, namely need not open top cover from cultivation pool to the coating seeded process of cultivating pilot trench, inclination angle that can be by modifier or carry out horizontal circumference and shake and finish the coating inoculation, reduced the false positive results that causes because of the environmental microorganism pollution, improved the stability of detection efficiency and detected result, also reduced the danger that strong pathogenic bacterium operation is brought, can make simultaneously and cultivate and the detection working scaleization;
(4) this device and cultivation thereof and detection method both can be used for the quick diagnosis discriminating of bacterium, can observe the biological chemistry characteristics of bacterial enzyme system complete again, traditional morphological specificity detection, microbial culture, biochemical test concentrated in the device carry out synchronously, simple to operate, can shorten time and efforts greatly;
(5) this apparatus structure is simple, easy to operate, can improve detection efficiency, and it is detachable, separate to cultivate pilot trench, the handiness that can improve experimental implementation; Recycle on the chassis, saves Financial cost.
Description of drawings
Fig. 1 is the assembling synoptic diagram of a kind of biochemical cultivation of the present invention with proofing unit (full open model cultivation pilot trench).
Fig. 2 is the schematic perspective view that chassis and full open model are cultivated pilot trench among Fig. 1.
Fig. 3 is the schematic perspective view on chassis among Fig. 2.
Fig. 4 is the schematic top plan view on chassis among Fig. 2.
Fig. 5 is the A-A cut-away view of Fig. 4.
Fig. 6 is the elevational schematic view on chassis among Fig. 2.
Fig. 7 is the schematic perspective view that full open model is cultivated the amplification of pilot trench among Fig. 1.
Fig. 8 is the schematic perspective view of the amplification of the open cultivation pilot trench of part.
Fig. 9 is the schematic perspective view of chassis and the open cultivation pilot trench of part.
Figure 10 is that a kind of biochemical cultivation of the present invention and proofing unit place the synoptic diagram on the shaking table equipment.
Wherein:
Tilt at the end 121
Periphery wall 124
Stiffening web 13
Cultivate pilot trench 2
Full open model is cultivated pilot trench 2a
The open cultivation pilot trench of part 2b
Shaking table equipment 4.
Embodiment
Referring to Fig. 1 to Fig. 9, a kind of biochemical cultivation and proofing unit of the present invention comprises the chassis 1 and the top cover 3 that are connected, and the cross section on described chassis 1 is circular or oval.Described chassis 1 comprises and is arranged at the recessed cultivation pool 11 of chassis 1 central authorities and is centered around annular disposal area 12 around the cultivation pool 11.
Described disposal area 12 comprises at the bottom of the inclination of tilting to cultivation pool 11 121, is arranged on several strip projected parts 122, internal perisporium 123 and the periphery wall 124 of the ends 121 upper surface.Turn up and be connected with the upper end of the internal perisporium 123 of disposal area in the upper edge of described cultivation pool 11, the upper edge of described cultivation pool 11 and the angle of horizontal plane are α, and the scope of α is that 0 degree is to 90 degree.The discovery of research and test, the optimum range of α angle are that 20 degree are to 45 degree.The α angle on the chassis 1 shown in the figure is 25 degree.At the bottom of the inclination of described disposal area 121 with the angle of horizontal plane be β, the scope of β angle is that 0 degree is to 90 degree.The β angle on the chassis 1 shown in the figure is 35 degree.Described protruding 122 center on cultivation pool 11 is radial arrangement, and disposal area 12 is separated into several fan-shaped warehouse compartments.The number of the warehouse compartment of disposal area 12 commonly used is 3～30.The warehouse compartment on the chassis 1 shown in the figure is 21.
Described chassis 1 also comprises several stiffening webs 13.Described stiffening web 13 is arranged on chassis 1 downside and vertical chassis 1, and described stiffening web 13 is radial arrangement around the outer wall of cultivation pool 11.The lower ends downward extension of the periphery wall 124 of described disposal area flushes with the bottom of cultivation pool 11.Described cultivation pool 11, disposal area 12 and stiffening web 13 one are connected.
Described chassis 1 is provided with several and cultivates pilot trench 2, and described cultivation pilot trench 2 is hollow sphenoid, cultivates pilot trench 2 narrow ends towards cultivation pool 11, is shelved in the warehouse compartment of disposal area 12.Twice rib places, the outside of described cultivation pilot trench 2 respectively are provided with half groove 22, and half of described groove 22 is connected with half of projection 122.The number of the cultivation pilot trench 2 of the chassis that is provided with 21 warehouse compartments 1 energy containing shown in the figure is no more than 21.
Described cultivation pilot trench 2 is cultivated pilot trench 2a or the open cultivation pilot trench of part 2b for full open model.Described full open model is cultivated the top of pilot trench 2a and is not established cover plate, and the top is open fully, as shown in Figure 7.The top of the open cultivation pilot trench of described part 2b is provided with a cover plate 21b, described cover plate 21b is trapezoidal, the length of described cover plate 21b is less than the notch length of cultivating pilot trench 2b, and described cover plate 21b is arranged on the narrow end of cultivating pilot trench 2b, and is closed fixing with the upper edge of cultivating pilot trench 2b.As Fig. 8, shown in Figure 9.
Full open model is cultivated pilot trench 2a energy containing solid medium, semisolid medium etc. and is detected substratum, and the open cultivation pilot trench of part 2b energy containing liquid nutrient medium, solid or semisolid medium etc. detect substratum.
The detection method of a kind of biochemical cultivation of the present invention and proofing unit may further comprise the steps:
Step 1: preparation liquid, solid, semisolid medium etc. detect substratum.Described detection substratum is one or more in selective medium, the biochemical differential medium; Or another kind of substratum, wherein can contain microbiotic, medicine, the compound of certain concentration known.
Step 2: the cultivation pilot trench 2 of above-mentioned detection substratum being inserted test design scheme correspondence.
Step 3: above-mentioned cultivation pilot trench 2 is placed on the warehouse compartment on apparatus of the present invention chassis 1 successively by the test design scheme.
Step 4: in cultivation pool 11, inject liquid nutrient medium.
Step 5: sample to be detected is seeded in the aforesaid liquid substratum.Described liquid nutrient medium is one or more of enrichment medium of common enrichment medium, selective enrichment medium or specified strain; Or another kind of substratum, wherein can contain microbiotic, medicine, the compound of certain concentration known.
Step 6: at a certain temperature, leave standstill and cultivate for some time, or low speed wave and culture for some time.Shake speed and do not spill cultivation pool 11 with liquid nutrient medium and be as the criterion, described shaking by hand-held this device shaken, and maybe this device placed on the shaking table equipment 4 and shakes.As shown in figure 10.
Step 7: said apparatus is shaken the coating inoculation, the liquid nutrient medium in the cultivation pool 11 is got rid of into cultivation pilot trench 2, the speed of shaking does not spill apparatus of the present invention with liquid nutrient medium and is as the criterion.Described shaking by hand-held this device shaken, and maybe this device placed on the shaking table equipment 4 and shakes.As shown in figure 10.
Step 8: add the required reagent of test or test paper;
Step 9: cultivation or low speed wave and culture are left standstill in the said apparatus placement at a certain temperature.Described shaking by hand-held this device shaken, and maybe this device placed on the shaking table equipment 4 and shakes.As shown in figure 10.
Step 10: with the naked eye or observation of use instrument and detect in the above-mentioned cultivation pilot trench 2 bacterium colony, cell colonies or organize the colony kind and muddy degree, the colour-change of form or mixed solution, or add detection reagent and further observe and detect.
Claims (8)
1. a biochemistry is cultivated and proofing unit, comprise the chassis (1) and the top cover (3) that are connected, it is characterized in that: described chassis (1) comprises and is arranged at the recessed cultivation pool (11) of chassis (1) central authorities and is centered around cultivation pool (11) annular disposal area (12) all around
Described disposal area (12) comprises (121) at the bottom of the inclination of tilting to cultivation pool (11), is arranged on several strip projected parts (122), internal perisporium (123) and the periphery wall (124) of the end (121) upper surface, turn up and be connected with the upper end of the internal perisporium (123) of disposal area in the upper edge of described cultivation pool (11), described projection (122) is radial arrangement around cultivation pool (11), and disposal area (12) are separated into several fan-shaped warehouse compartments;
Described chassis (1) is provided with several and cultivates pilot trench (2), described cultivation pilot trench (2) is hollow sphenoid, cultivate the narrow end of pilot trench (2) towards cultivation pool (11), be shelved in the warehouse compartment of disposal area (12), described cultivation pilot trench (2) is cultivated pilot trench (2a) or the open cultivation pilot trench of part (2b) for full open model, described full open model is cultivated the top of pilot trench (2a) and is not established cover plate, the top of the open cultivation pilot trench of described part (2b) is provided with a cover plate (21b), described cover plate (21b) is trapezoidal, the length of described cover plate (21b) is less than the notch length of cultivating pilot trench (2b), described cover plate (21b) is arranged on the narrow end of cultivating pilot trench (2b), and is closed fixing with the upper edge of cultivating pilot trench (2b).
2. a kind of biochemistry according to claim 1 is cultivated and proofing unit, it is characterized in that: the cross section of described chassis (1) is for circular or oval.
3. a kind of biochemistry according to claim 1 is cultivated and proofing unit, and it is characterized in that: the upper edge of described cultivation pool (11) and the angle of horizontal plane are α, and the scope of α is that 0 degree is to 90 degree.
4. according to claim 1 or 3 described a kind of biochemical cultivations and proofing unit, it is characterized in that: the upper edge of described cultivation pool (11) and the angle of horizontal plane are α, and the scope of α is that 20 degree are to 45 degree.
5. a kind of biochemistry according to claim 1 is cultivated and proofing unit, and it is characterized in that: the number of the warehouse compartment of described disposal area (12) is 3～30.
6. a kind of biochemistry according to claim 1 is cultivated and proofing unit, and it is characterized in that: twice rib places, the outside of described cultivation pilot trench (2) respectively are provided with half groove (22), and half of described groove (22) is connected with half of projection (122).
7. a kind of biochemistry according to claim 1 is cultivated and proofing unit, it is characterized in that: (121) are β with the angle of horizontal plane at the bottom of the inclination of described disposal area, and the scope of β is that 0 degree is to 90 degree.
8. a kind of biochemistry according to claim 1 is cultivated and proofing unit, it is characterized in that: the lower ends downward extension of the periphery wall of described disposal area (124) flushes with the bottom of cultivation pool (11).
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210269999 CN102816683B (en) | 2012-08-01 | 2012-08-01 | Biochemical culture and detection device as well as detection method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210269999 CN102816683B (en) | 2012-08-01 | 2012-08-01 | Biochemical culture and detection device as well as detection method thereof |
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| CN102816683A CN102816683A (en) | 2012-12-12 |
| CN102816683B true CN102816683B (en) | 2013-10-09 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI540207B (en) * | 2014-01-20 | 2016-07-01 | 國立清華大學 | Device and method for bacterial culture and assay |
| CN106047679B (en) * | 2016-05-31 | 2018-05-22 | 孙术杰 | Microorganism concn detection device and its application method in a kind of water of electrical control |
| TWI715176B (en) * | 2019-08-30 | 2021-01-01 | 台達電子工業股份有限公司 | Biological detection cartridge and method |
| CN114487452B (en) * | 2021-12-30 | 2022-09-02 | 河南黄河科技学院附属医院 | Automatic analyzer for bacterial culture drug sensitivity based on antibiotic concentration gradient |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2107469U (en) * | 1991-05-18 | 1992-06-17 | 彭钧 | Centrifugal bacterial biochemical, medicine sensitive and anaerobic experiment device |
| CN2263345Y (en) * | 1996-02-17 | 1997-09-24 | 董庆书 | Disposable bacterial biochemical identification, medicinal sensitive quantitative detecting plate |
| ATE366301T1 (en) * | 2001-02-05 | 2007-07-15 | Millipore Corp | DETECTION OF MICROORGANISMS |
| CN2923720Y (en) * | 2005-07-21 | 2007-07-18 | 高春平 | Automatic bacteria detection system |
| CN201313888Y (en) * | 2008-12-15 | 2009-09-23 | 杭州电子科技大学 | Microbe inoculation-cultivation device |
| CN102703567B (en) * | 2012-06-15 | 2013-10-09 | 江苏嘉语生物医药技术有限公司 | Multi-phase co-culture and detection method and device thereof |
| CN202730110U (en) * | 2012-08-01 | 2013-02-13 | 江苏嘉语生物医药技术有限公司 | Biochemical culturing and detecting device |
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Owner name: JIAXING JIESAI BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: JIANGSU JIAYU BIOLOG MEDICAL TECHNOLOGY CO., LTD. Effective date: 20140520 |
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Free format text: CORRECT: ADDRESS; FROM: 214431 WUXI, JIANGSU PROVINCE TO: 314400 JIAXING, ZHEJIANG PROVINCE |
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Effective date of registration: 20140520 Address after: 314400 Zhejiang city in Haining Province Economic Development Zone Haining Double Road No. 128, building 5, 515 branch room 517 Patentee after: Jiaxing Jie Biotechnology Co., Ltd. Address before: 214431 B207 room, No. 85 sanding Road, Wuxi, Jiangsu, Jiangyin Patentee before: Jiangsu Jiayu Biomedicine Technology Co.,Ltd. |