CN102735636B - Optical device for biochemical analyzer - Google Patents
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- CN102735636B CN102735636B CN201210225260.XA CN201210225260A CN102735636B CN 102735636 B CN102735636 B CN 102735636B CN 201210225260 A CN201210225260 A CN 201210225260A CN 102735636 B CN102735636 B CN 102735636B
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Abstract
The invention discloses an optical device for a biochemical analyzer. The optical device comprises a light source, a front condenser lens, a reaction disc for placing a reaction cup, a constant temperature bath for maintaining the temperature of a reaction solution in the reaction cup, a rear beam splitting lens and an optical collection point. The front condenser lens comprises a biconvex lens and a meniscus lens with the biconvex lens being in the front and the meniscus lens being at the back, and the convex surface of the meniscus lens is a plane of incidence. The rear beam splitting lens comprises two same planoconvex lenses, convex surfaces of which are attached to each other. According to the invention, through redesigning parts of the optical device and adjusting optical path, the minimum reaction volume is reduced such that reagent consumption and blood consumption of the biochemical analyzer are greatly minimized and reagent usage cost in a hospital is decreased.
Description
Technical field
The present invention relates to biomedical devices technical field, refer to especially a kind of Biochemical Analyzer optical devices.
Background technology
Minimal reaction volume in Biochemical Analyzer is a very important parameter, and it is the liquid volume of minimum needs in primary first-order equation.Minimal reaction volume is the potpourri of the body fluid (being mainly blood) of reagent and human body, minimal reaction volume is less, the blood of human body of required extraction and needed reagent are just fewer, obviously blood drawing reduces the misery that can reduce patient, required reagent reduce can greatly save Biochemical Analyzer use cost.
But the approach dwindling of minimal reaction volume dwindles light exactly by reaction cup volume and spot definition, and this has proposed very high requirement for optical devices.
At present, the reaction cup cross-sectional area of general Biochemical Analyzer is 5 × 6mm
2, spot definition is 3 × 1mm
2, minimal reaction volume is 150 μ l, and if minimal reaction volume is reduced to 50 μ l, just can reduce the painful of patient and greatly save Biochemical Analyzer use cost.
Summary of the invention
In view of this, the object of the invention is to propose a kind of Biochemical Analyzer optical devices, it can reduce the minimal reaction volume of Biochemical Analyzer.
Based on above-mentioned purpose a kind of Biochemical Analyzer optical devices provided by the invention, comprising: the reaction tray of light source, front collector lens, placing response cup, keep calibration cell, rear beam splitting lens, the light collection point of reacting liquid temperature in described reaction cup; Described front collector lens comprises a slice biconvex lens and a slice concave-convex lens, and the biconvex lens of described front collector lens is front, and concave-convex lens is rear, and described concave-convex lens convex surface is the plane of incidence; Described rear beam splitting lens comprises two identical plano-convex lenss, and the convex surface of two lens is affixed; Described incident light sends and passes through successively described biconvex lens, concave-convex lens, calibration cell, constant temperature liquid, reaction cup, reactant liquor from light source, described emergent light passes through described reactant liquor, reaction cup, constant temperature liquid, calibration cell successively, and two identical plano-convex lenss being affixed of convex surface, be finally mapped to described light collection point.
In one embodiment, described reaction cup is 5mm along the inwall spacing of described reaction tray radial direction, and described reaction cup wall thickness is not more than 0.75mm, is not more than 4mm along the inwall spacing of described reaction tray tangential direction; Described calibration cell wall thickness is not less than 1mm, and calibration cell is not more than 15.5mm along the outer wall spacing of described reaction tray radial direction.
In another embodiment, described reaction cup is 4mm along the inwall spacing of described reaction tray tangential direction.
In another embodiment, described calibration cell is 15.5mm along the outer wall spacing of described reaction tray radial direction, and described reaction cup wall thickness is 0.75mm, and described calibration cell wall thickness is 1mm.
In another embodiment, the bob amount sum that described reaction tray rotates a circle is not more than 1.5mm.
In another embodiment, the biconvex lens plane of incidence radius-of-curvature of described front collector lens is 15.4mm, and exit facet radius-of-curvature is 23.6mm, and thickness is 1.7mm; The concave-convex lens plane of incidence radius-of-curvature of described front collector lens is 4.7mm, and exit facet radius-of-curvature is 11mm, and thickness is 2mm.
In another embodiment, the spacing of the biconvex lens exit facet of described front collector lens and the concave-convex lens plane of incidence of described front collector lens is 6.6mm, and the concave-convex lens exit facet of described front collector lens and the spacing of described calibration cell outer wall are 2mm.
In another embodiment, the convex surface radius of the plano-convex lens of described rear beam splitting lens is 9.1mm, and thickness is 3mm.
In another embodiment, the front plano-convex lens plane of incidence of described rear beam splitting lens and the spacing of described calibration cell outer wall are 5mm.
In another embodiment, the biconvex lens plane of incidence of described front collector lens and the spacing of optical devices light source are 30mm, and the spacing of the rear plano-convex lens exit facet of described rear beam splitting lens and the light collection point of optical devices is 24mm.
In another embodiment, the making material of described reaction cup, calibration cell, front collector lens, rear beam splitting lens is optical glass.
As can be seen from above, a kind of Biochemical Analyzer optical devices provided by the invention, by the parts in optical devices are redesigned and adjust light path, thereby reduce minimal reaction volume, make this kind of Biochemical Analyzer greatly reduce reagent consumption and blood using amount, reduce the cost that hospital is used reagent, and then reduce the amount of clinical waste discharge, reduce the difficulty of MEDICAL WASTE TREATMENT; Reduce assay office needs blood volume simultaneously, reduces patient's misery, improves patient's level of comfort, reduces patient's blood drawing pressure.
Brief description of the drawings
Fig. 1 be in Biochemical Analyzer disclosed by the invention optical devices embodiment light path the each several part front view of process;
Fig. 2 is reaction cup vertical view in the embodiment of the present invention.
Embodiment
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with specific embodiment, and with reference to accompanying drawing, the present invention is described in more detail.
A kind of Biochemical Analyzer optical devices disclosed in this invention, comprising: light source, front collector lens, place described reaction cup reaction tray, keep calibration cell, rear beam splitting lens, the light collection point of reacting liquid temperature in described reaction cup; Described front collector lens comprises a slice biconvex lens and a slice concave-convex lens, and the biconvex lens of described front collector lens is front, and concave-convex lens is rear, and described concave-convex lens convex surface is the plane of incidence; Described rear beam splitting lens comprises two identical plano-convex lenss, and the convex surface of two lens is affixed.
Described incident light sends and passes through successively described biconvex lens, concave-convex lens, calibration cell, constant temperature liquid, reaction cup, reactant liquor from light source, described emergent light passes through described reactant liquor, reaction cup, constant temperature liquid, calibration cell successively, and two identical plano-convex lenss being affixed of convex surface, be finally mapped to described light collection point.
With reference to figure 1, Biochemical Analyzer disclosed by the invention with light path in optical devices embodiment the each several part front view of process; In Fig. 1, only drawn light path the part of each parts of process, because incident light sectional area is little, emergent light sectional area is large, therefore varying in size of two-part part, left and right.
Wherein, described optical devices comprise: light source, front collector lens 1, reaction cup 2, calibration cell 4, rear beam splitting lens 6, light collection point; Wherein, reaction cup 2 is arranged in calibration cell 4.
Described incident light from light source send and pass through successively described biconvex lens, concave-convex lens, calibration cell 4, constant temperature liquid 5, reaction cup 2, the reactant liquor 3 of collector lens 1; Described emergent light passes through described reactant liquor 3, reaction cup 2, constant temperature liquid 5, calibration cell 4 successively, and two identical plano-convex lenss that the convex surface of beam splitting lens 6 is affixed afterwards, is finally mapped to described light collection point.
The wall thickness d of described reaction cup 2
3for 0.75mm, the wall thickness d of described calibration cell 4
6for 1mm; Described reaction cup 2 is along the inwall spacing d of described reaction tray radial direction (passing through direction along light)
1for 5mm, described reaction cup 2 is along the inwall spacing d of described reaction tray tangential direction
2(accompanying drawing 2) is 4mm, and described calibration cell 4 is along the outer wall spacing d of described reaction tray radial direction (passing through direction along light)
5for 15.5mm.
Described front collector lens 1 comprises a slice biconvex lens and a slice concave-convex lens, and the biconvex lens of described front collector lens 1 is front, and concave-convex lens is rear, and described concave-convex lens convex surface is the plane of incidence; Described rear beam splitting lens 6 comprises two identical plano-convex lenss, and the convex surface of two lens is affixed.The biconvex lens plane of incidence radius-of-curvature of described front collector lens 1 is 15.4mm, and exit facet radius-of-curvature is 23.6mm, thickness d
7for 1.7mm; The concave-convex lens plane of incidence radius-of-curvature of described front collector lens 1 is 4.7mm, and exit facet radius-of-curvature is 11mm, thickness d
9for 2mm.The convex curvature radius of the plano-convex lens of described rear beam splitting lens 6 is 9.1mm, thickness d
12for 3mm.
The spacing d of the biconvex lens exit facet of described front collector lens 1 and the concave-convex lens plane of incidence of described front collector lens
8for 6.6mm, the concave-convex lens exit facet of described front collector lens 1 and the spacing d of described calibration cell outer wall
10for 2mm.The front plano-convex lens plane of incidence of described rear beam splitting lens 6 and the spacing d of described calibration cell outer wall
11for 5mm.
The biconvex lens plane of incidence of described front collector lens 1 and the spacing of optical devices light source are 30mm, and the spacing of the rear plano-convex lens exit facet of described rear beam splitting lens 6 and the light collection point of optical devices is 24mm.
When test, light penetrates from light source, after the biconvex lens of premenstrual collector lens 1 and concave-convex lens optically focused, the cell wall, constant temperature liquid 5, the wall of cup of reaction cup 2, the reactant liquor 3 that pass through successively calibration cell 4, under illumination, reactant liquor reacts, emergent light afterwards, pass through successively again wall of cup, the constant temperature liquid 5 of reaction cup 2, the cell wall of calibration cell 4, again through after after two identical plano-convex lenss being affixed of the convex surface of beam splitting lens 6 disperse, be mapped to light collection point, after opto-electronic conversion, test.
Adopt after above-mentioned optical devices, see through the luminous point height d of reaction cup 2
4narrow down to 1.5mm, spot width (along reaction tray tangential direction) narrows down to 0.5mm.
Described reaction tray can be rotated perpendicular to the axle of optical axis at horizontal winding in test process, but generally this rotation can not be abswolute level, therefore will cause in its rotary course and produce bob, in the maximum that reaction tray rotates a circle, jerk value and maximum lower jerk value sum are the maximum bob amount sum rotating a circle.Obviously, this beating will impact test process.Under normal circumstances, described reaction tray is by design bearing and make material selection, can make its maximum bob amount sum rotating a circle be no more than 1.5mm, has ensured the stability of reaction tray.
Consider the surface tension of reactant liquor 3, will make reactant liquor 3 increase along wall of cup near the liquid level at reaction cup 2 wall of cup places, reactant liquor 3 can decline accordingly at the liquid level of reaction cup 2 centers.The position of irradiating due to luminous point is at the center of reactant liquor 3, and reactant liquor 3 center liquid levels decline and may cause luminous point cannot be irradiated to completely on reactant liquor 3, so just can cause the error of testing result.In order to compensate the height of the reactant liquor center liquid level reducing because of liquid level tension force, in the time adding reactant liquor, need to consider to increase suitable reactant liquor volume.
Therefore, the theoretical calculation formula of the minimal reaction volume of Biochemical Analyzer is:
Inside surface cross-sectional area × (the luminous point height d of minimal reaction volume=reaction cup 2
4the height that maximum bob amount sum+reactant liquor center liquid level that+reaction tray rotates a circle loses because of surface tension)
Wherein, the inside surface cross-sectional area of reaction cup 2 is d
1× d
2=5mm × 4mm=20mm
2, the maximum bob amount sum that reaction tray rotates a circle is 1.5mm, luminous point height d
4for 1.5mm, the height that reactant liquor center liquid level loses because of surface tension is 1.5mm(theoretical value):
Minimal reaction volume=5 × 4 × (1.5+1.5+1.5) mm
3=90 μ l, if consider the impact of equipment error, minimal reaction volume is 100 μ l.
More than, for utilizing the theoretical value of the minimal reaction volume that optical devices of the present invention measure under theoretical calculating, prove below by experiment.
Experiment material: a set of complete biochemical analysis instrument apparatus, comprises 6 half tables of signal acquisition circuit plate and signals collecting instrument.Reaction tray is to be become by 8 cup joint groups, and each cup is associated with 20 cup positions, and for convenient, we only record the tenth cup position in each glass of connection, have eight data.
Experimental technique: first will add potassium dichromate (K to be measured in each reaction cup
2gr
2+ O
7) solution, the absorbance of this potassium dichromate first configures, and is about 1.0 left and right.
In 160 all reaction cup, add this kind of solution 90 μ l, measure absorbance.Measured value and theoretical value are contrasted, if test value and theoretical value differ large (error is beyond 0.02), increase 10 μ l liquor capacity to be measured, continue to measure, until differ in error range with theoretical value.
Absorbance theoretical value is 0.998.
Experimental data is in table 1.
Table 1
From data, can draw, when reactant liquor volume is 100 μ l, can obtain the test value basically identical with theoretical value.
To sum up can draw, the minimal reaction volume that adopts the Biochemical Analyzer of optical devices of the present invention is 100 μ l, has reduced 1/3rd than general Biochemical Analyzer minimal reaction volume 150 μ l.
As can be seen from the above, Biochemical Analyzer optical devices disclosed in this invention, described reaction cup, along the inwall spacing of described reaction tray radial direction (being the direction that light passes through), still keeps the size of 5mm, by distance, realize good light absorption to ensure enough light; And along the inwall hypotelorism of described reaction tray tangential direction to 4mm, the cumulative volume of reaction cup is dwindled much, the sectional area of the conventional 5mm × 6mm selecting of contrast, this change of single sectional area just by minimal reaction volume-diminished 1/3rd.
Rear beam splitting lens 6 is for light splitting object thereafter and use, and has adopted two identical plano-convex lens eyeglasses to save cost.
A kind of Biochemical Analyzer optical devices disclosed by the invention, by adopting the reaction cup of less xsect, the less spot size that sees through reaction cup, and the parts in optical devices are redesigned and adjust light path, realize less minimal reaction volume, made to adopt the Biochemical Analyzer of this kind of optical devices to greatly reduce reagent consumption and blood using amount, reduced the cost of hospital's use reagent, and then reduce the amount that clinical waste discharges, reduce the difficulty of MEDICAL WASTE TREATMENT; Reduce assay office needs blood volume simultaneously, reduces patient's misery, improves patient's level of comfort, reduces patient's blood drawing pressure.
It needs to be noted, reaction cup cross-sectional area in above-described embodiment is 5 × 4, wherein can also dwindle again along the inwall spacing of described reaction tray tangential direction, can make in theory minimal reaction volume less, consider the debugging of described optical devices and testing apparatus each several part, and the problem of test result precision, adopt the size of 4mm here, but do not got rid of the possibility that adopts 3.5mm or less size.
In addition, calibration cell in above-described embodiment is 15.5mm along the outer wall spacing of described reaction tray radial direction, in this distance theory, also can dwindle, equally, consider the debugging of described optical devices and testing apparatus each several part, and the problem of test result precision, adopt the size of 15.5mm here, but do not got rid of the possibility that adopts 15mm or less size.
Certainly, the design of described reaction cup, the wall thickness of calibration cell neither be confined to 0.75mm and 1mm, suitably reduces reaction cup wall thickness and increases calibration cell wall thickness, also belongs to protection scope of the present invention.
Front collector lens in described optical devices and the design of rear beam splitting lens design according to reaction cup size and spot definition; therefore; after described reaction cup size and spot definition variation; the corresponding variation that also can cause spacing between front collector lens and rear beam splitting lens and each several part, meets accordingly the front collector lens of the present invention's design and the design of rear beam splitting lens and also belongs to protection scope of the present invention.
The present invention can adopt comparatively simple and conventional design, as as described in calibration cell be water bath, the making material of described reaction cup, front collector lens and rear beam splitting lens is optical glass, particularly K9 glass, but other materials is also feasible, as long as described optical devices and testing apparatus are carried out to corresponding debugging.
The maximum bob amount sum that above-mentioned reaction tray rotates a circle is 1.5mm, certainly, selects through special design bearing and making material, and this bob amount sum can also be less, meanwhile, and can also be by minimal reaction volume-diminished.
Those of ordinary skill in the field are to be understood that: the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any amendment of making, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (11)
1. Biochemical Analyzer optical devices, comprise light source, front collector lens, placing response cup reaction tray, keep calibration cell, rear beam splitting lens, the light collection point of reacting liquid temperature in described reaction cup; It is characterized in that, described front collector lens comprises a slice biconvex lens and a slice concave-convex lens, and the biconvex lens of described front collector lens is front, and concave-convex lens is rear, and described concave-convex lens convex surface is the plane of incidence; Described rear beam splitting lens comprises two identical plano-convex lenss, and the convex surface of two lens is affixed; Incident light sends and passes through successively described biconvex lens, concave-convex lens, calibration cell, constant temperature liquid, reaction cup, reactant liquor from light source, emergent light passes through described reactant liquor, reaction cup, constant temperature liquid, calibration cell successively, and two identical plano-convex lenss being affixed of convex surface, be finally mapped to described light collection point.
2. Biochemical Analyzer optical devices according to claim 1, it is characterized in that, described reaction cup is 5mm along the inwall spacing of described reaction tray radial direction, and described reaction cup wall thickness is not more than 0.75mm, is not more than 4mm along the inwall spacing of described reaction tray tangential direction; Described calibration cell wall thickness is not less than 1mm, and calibration cell is not more than 15.5mm along the outer wall spacing of described reaction tray radial direction.
3. Biochemical Analyzer optical devices according to claim 2, is characterized in that, described reaction cup is 4mm along the inwall spacing of described reaction tray tangential direction.
4. Biochemical Analyzer optical devices according to claim 3, is characterized in that, described calibration cell is 15.5mm along the outer wall spacing of described reaction tray radial direction, and described reaction cup wall thickness is 0.75mm, and described calibration cell wall thickness is 1mm.
5. Biochemical Analyzer optical devices according to claim 4, is characterized in that, the bob amount sum that described reaction tray rotates a circle is not more than 1.5mm.
6. Biochemical Analyzer optical devices according to claim 5, is characterized in that, the biconvex lens plane of incidence radius-of-curvature of described front collector lens is 15.4mm, and exit facet radius-of-curvature is 23.6mm, and thickness is 1.7mm; The concave-convex lens plane of incidence radius-of-curvature of described front collector lens is 4.7mm, and exit facet radius-of-curvature is 11mm, and thickness is 2mm.
7. Biochemical Analyzer optical devices according to claim 6, it is characterized in that, the spacing of the biconvex lens exit facet of described front collector lens and the concave-convex lens plane of incidence of described front collector lens is 6.6mm, and the concave-convex lens exit facet of described front collector lens and the spacing of described calibration cell outer wall are 2mm.
8. Biochemical Analyzer optical devices according to claim 7, is characterized in that, the convex surface radius of the plano-convex lens of described rear beam splitting lens is 9.1mm, and thickness is 3mm.
9. Biochemical Analyzer optical devices according to claim 8, is characterized in that, the front plano-convex lens plane of incidence of described rear beam splitting lens and the spacing of described calibration cell outer wall are 5mm.
10. Biochemical Analyzer optical devices according to claim 9, it is characterized in that, the biconvex lens plane of incidence of described front collector lens and the spacing of optical devices light source are 30mm, and the spacing of the rear plano-convex lens exit facet of described rear beam splitting lens and the light collection point of optical devices is 24mm.
11. according to the Biochemical Analyzer optical devices described in claim 1 to 10 any one, it is characterized in that, the making material of described reaction cup, calibration cell, front collector lens, rear beam splitting lens is optical glass.
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CN111022967A (en) * | 2019-11-25 | 2020-04-17 | 中国科学院苏州纳米技术与纳米仿生研究所 | Regional light source dodging structure applied to fluorescence imaging system and fluorescence imaging system |
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