CN102631717B - Device for in-vitro induction of self peripheral blood of patients to generate high-concentration anti-inflammatory factors - Google Patents
Device for in-vitro induction of self peripheral blood of patients to generate high-concentration anti-inflammatory factors Download PDFInfo
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- CN102631717B CN102631717B CN2012101212376A CN201210121237A CN102631717B CN 102631717 B CN102631717 B CN 102631717B CN 2012101212376 A CN2012101212376 A CN 2012101212376A CN 201210121237 A CN201210121237 A CN 201210121237A CN 102631717 B CN102631717 B CN 102631717B
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- sleeve pipe
- peripheral blood
- inflammatory factors
- piston
- centrifuge tube
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- 230000003110 anti-inflammatory effect Effects 0.000 title claims abstract description 25
- 210000005259 peripheral blood Anatomy 0.000 title claims abstract description 25
- 239000011886 peripheral blood Substances 0.000 title claims abstract description 25
- 238000000338 in vitro Methods 0.000 title abstract 3
- 230000006698 induction Effects 0.000 title abstract 3
- 230000000763 evoking effect Effects 0.000 claims description 18
- 238000001914 filtration Methods 0.000 claims description 14
- 239000000945 filler Substances 0.000 claims description 6
- 239000011324 bead Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 210000004369 blood Anatomy 0.000 abstract description 12
- 239000008280 blood Substances 0.000 abstract description 12
- 238000011109 contamination Methods 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 description 15
- 210000003677 hemocyte Anatomy 0.000 description 6
- 229940000351 hemocyte Drugs 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- 201000008482 osteoarthritis Diseases 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
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Abstract
The invention relates to a device for in-vitro induction of self peripheral blood of patients to generate high-concentration anti-inflammatory factors. The device is characterized in that the device comprises a vacuum aspirator I (1), a centrifuge tube (2) and a vacuum aspirator B (3); the vacuum aspirator I (1) comprises a sleeving I (1.1) and a piston I (1.2); the piston I (1.2) is arranged in the sleeving I (1.1) and a flexible pipe (1.3) penetrates into the piston I (1.2); the vacuum aspirator II (3) comprises a sleeving II (3.1) and a piston II (3.2); the piston II (3.2) is arranged in the sleeving II (3.1) and the bottoms of the sleeving I (1.1) and the sleeving II (3.1) can be in screw-thread fit with the top of the centrifuge tube (2). The device for in-vitro induction of self peripheral blood of patients to generate high-concentration anti-inflammatory factors can effectively reduce the contact between blood and air and the possibility of bacterial contamination and is simple and convenient in operation.
Description
Technical field
The present invention relates to the device that a kind of external evoked patient self peripheral blood produces the high concentration anti-inflammatory factors, for osteoarthritis treatment.
Background technology
Human peripheral blood can produce the serum that contains the high concentration anti-inflammatory factors external after inducing, this antiinflammatory serum is recycled in patient's articular cavity, can stop further developing and significantly improving symptom of the osteoarthritis state of an illness.
This process relates to syringe and extracts patient's peripheral blood; Peripheral blood is injected to the container with induced material; Blood in container makes it produce anti-inflammatory factors in external evoked cultivation; Adopt centrifugal method that serum is separated with hemocyte; Draw the serum part and installed to juxtaposition cryopreservation in the preservation pipe in rear minute.Whole process need is used multiple device, organically coordinates and lack between these existing apparatus, makes operation become complicated, is difficult for grasping.In addition, a plurality of steps relate to blood or serum contacts with air, easily cause germ contamination.
Summary of the invention
Purpose of the present invention structurally overcomes above-mentioned deficiency, provides a set of complete external evoked patient self peripheral blood to produce the device of high concentration anti-inflammatory factors.This covering device can avoid preparing the inconvenience that multiple dispersal device brings; The each several part reasonable combination of device, easy to operate; Reduce the chance contacted of blood and air, reduced the probability of germ contamination; Can induce patient self peripheral blood in external generation high concentration anti-inflammatory factors.
The object of the present invention is achieved like this:
The external evoked patient of the present invention self peripheral blood produces the device of high concentration anti-inflammatory factors, it comprises the vacuum suction apparatus I, centrifuge tube and vacuum suction apparatus II, described vacuum suction apparatus I comprises sleeve pipe I and piston I, described piston I is located in the sleeve pipe I, in described piston I, be installed with flexible pipe, the top of described centrifuge tube is with lid, described vacuum suction apparatus II comprises sleeve pipe II and piston II, described piston II is located in the sleeve pipe II, the bottom of described sleeve pipe II is provided with suction attachment, the bottom of described suction attachment is connected to suction pipe, described sleeve pipe I and sleeve pipe II bottom all can coordinate with the screw top of centrifuge tube.
The bottom of described sleeve pipe I is protruded and is provided with the female thread I, and the bottom of described sleeve pipe II is protruded and is provided with the female thread II, and the top end opening of described centrifuge tube also is provided with external screw thread, and female thread I, female thread II all can coordinate with external screw thread.
On described lid, have air-vent, described sleeve pipe II lower sides has filtering holes, in air-vent and filtering holes, is respectively equipped with air filter film and air filtration filler, and the aperture of air filter film and air filtration filler all is less than 0.22 μ m.
The centre of described sleeve pipe I also is provided with spacing ring.
The outer race of described suction attachment has germ tight filter.
The bottom of described suction pipe is bent upwards into hook-shaped.
The bottom of described centrifuge tube is placed with some glass beads.
The device that the external evoked patient of the present invention self peripheral blood produces the high concentration anti-inflammatory factors has the following advantages:
The device that this external evoked patient self peripheral blood produces the high concentration anti-inflammatory factors changes vacuum suction apparatus I, centrifuge tube and vacuum suction apparatus II, centrifuge tube into removable structure, the blood that the vacuum suction apparatus I is extracted out can be delivered directly to and be preinstalled with in the centrifuge tube of inducing peripheral blood to produce the anti-inflammatory factors material, after subsequently the vacuum suction apparatus I being taken apart, just can directly carry out external evoked cultivation to the blood in centrifuge tube, effectively reduce blood and contacted with air.It is centrifugal that the rear centrifuge tube of cultivation end can directly be put centrifuge, and serum is separated with hemocyte.Then use the vacuum suction apparatus II by the serum sucking-off in centrifuge tube, reinject in the serum keeping pipe, so also reduced the chance that blood contacts with air, thereby reduce the possibility of germ contamination, also can sterilizing filter be installed in suction attachment place of vacuum suction apparatus II, serum is filtered, remove possibility residual hemocyte and antibacterial.Whole operating process is simple and convenient, and without preparing more equipment, the operating time is shorter.
The accompanying drawing explanation
Fig. 1 is the fiting relation figure that the external evoked patient of the present invention self peripheral blood produces vacuum suction apparatus I and centrifuge tube in the device of high concentration anti-inflammatory factors.
Fig. 2 is the structural representation of centrifuge tube in Fig. 1.
Fig. 3 is the fiting relation figure that the external evoked patient of the present invention self peripheral blood produces vacuum suction apparatus II and centrifuge tube in the device of high concentration anti-inflammatory factors.
Fig. 4 is the structural representation of vacuum suction apparatus II in Fig. 3.
In figure: vacuum suction apparatus I 1, sleeve pipe I 1.1, piston I 1.2, flexible pipe 1.3, female thread I 1.4, spacing ring 1.5;
Vacuum suction apparatus II 3, sleeve pipe II 3.1, piston II 3.2, female thread II 3.3, suction attachment 3.4, suction pipe 3.5, germ tight filter 3.6, filtering holes 3.7;
Serum keeping pipe 4.
The specific embodiment
Referring to Fig. 1 to Fig. 4, the present invention relates to the device that a kind of external evoked patient self peripheral blood produces the high concentration anti-inflammatory factors, it comprises vacuum suction apparatus I 1, centrifuge tube 2 and vacuum suction apparatus II 3, described vacuum suction apparatus I 1 comprises sleeve pipe I 1.1 and piston I 1.2, described piston I 1.2 is located in sleeve pipe I 1.1, and two ends are exposed to sleeve pipe I 1.1, can in sleeve pipe I 1.1, move up and down, described piston Ⅰ1.2De center is installed with a flexible pipe 1.3, the two ends of flexible pipe 1.3 are exposed to piston I 1.2, its upper end with venipuncture needle, join, the bottom of described sleeve pipe I 1.1 is protruded and is provided with female thread I 1.4, the centre of described sleeve pipe I 1.1 also is provided with a spacing ring 1.5, this spacing ring 1.5 can be spacing to the bottom of piston I 1.2, when piston I 1.2 moves up blood drawing, spacing ring 1.5 can block piston I 1.2, guarantee to extract the blood of fixed volume, simultaneously can prevent that it is pulled out sleeve pipe I 1.1.
The top end opening of described centrifuge tube 2 is provided with external screw thread 2.1, the bottom of centrifuge tube 2 is placed with some glass beads 2.2, described glass bead 2.2 can induce peripheral blood to produce the anti-inflammatory factors of high concentration, the top of centrifuge tube 2 is with lid 2.3, on described lid 2.3, have air-vent 2.4, be provided with air filter film in this air-vent 2.4, the aperture of air filter film is less than 0.22 μ m, to allow air free to pass through, and prevent that antibacterial from passing through.
Described vacuum suction apparatus II 3 comprises sleeve pipe II 3.1 and piston II 3.2, described piston II 3.2 is located in sleeve pipe II 3.1, and upper end is exposed to sleeve pipe II 3.1, can in sleeve pipe II 3.1, move up and down, the bottom of described sleeve pipe II 3.1 is protruded and is provided with female thread II 3.3, the bottom of described sleeve pipe II 3.1 is provided with suction attachment 3.4, the bottom of described suction attachment 3.4 is connected to suction pipe 3.5, the bottom of described suction pipe 3.5 is bent upwards into hook-shaped, the outer race of described suction attachment 3.4 has germ tight filter 3.6, this germ tight filter 3.6 can be removed hemocyte that may be residual in serum and the antibacterial that may pollute, on the sidewall of described sleeve pipe II 3.1 bottoms near female thread II 3.3 places, have filtering holes 3.7, when this filtering holes 3.7 can be avoided vacuum suction apparatus II 3 suction in the interior formation negative pressure of sleeve pipe II 3.1, in described filtering holes 3.7, be provided with the air filtration filler, the aperture of air filtration filler is less than 0.22 μ m, to allow air free to pass through, and prevent that antibacterial from passing through.
Described sleeve pipe I 1.1 bottoms can coordinate with the external screw thread 2.1 of centrifuge tube 2 by the female thread I, and sleeve pipe II 3.1 bottoms also can coordinate with the external screw thread 2.1 of centrifuge tube 2 by female thread II 3.3.
The device of this external evoked patient self peripheral blood generation high concentration anti-inflammatory factors in use, the sleeve pipe I of vacuum suction apparatus I 1 is threaded connection with centrifuge tube 2, only flexible pipe 1.3 need be connected to venipuncture needle, after the insertion blood vessel, pull up piston I 1.2 blood directly is drawn in centrifuge tube 2.After exhausting, the sleeve pipe I is separated, 2.3 pairs of blood of cover lid carry out inducing culture.Finally lid 2.3 is opened, the sleeve pipe II of vacuum suction apparatus II 3 3.1 and centrifuge tube 2 screw threads are joined, just can serum be taken out by suction pipe 3.5, the crooked upwards setting of end due to suction pipe 3.5, suction pipe 3.5 just in time is positioned at the top of hemocyte face, can be to the interference of cell generation of interfaces and by the hemocyte sucking-off.After sucking, then serum is injected to the serum keeping pipe 4 rearmounted low temperature of sealing (80 C °) and deposit.
Vacuum suction apparatus I 1 and centrifuge tube 2 are assembled into one in advance, vacuum suction apparatus II 3 and suction pipe 3.5 are assembled into one in advance, these two parts and lid 2.3, germ tight filter 3.6, serum keeping pipe 4 are divided in the plastic bag of good seal, take oxirane or radiation sterilization to carry out sterilizing.
Claims (6)
1. external evoked patient self peripheral blood produces the device of high concentration anti-inflammatory factors, it is characterized in that: it comprises vacuum suction apparatus I (1), centrifuge tube (2) and vacuum suction apparatus II (3), described vacuum suction apparatus I (1) comprises sleeve pipe I (1.1) and piston I (1.2), described piston I (1.2) is located in sleeve pipe I (1.1), in described piston I (1.2), be installed with flexible pipe (1.3), the top of described centrifuge tube (2) is with lid (2.3), on described lid (2.3), have air-vent (2.4), described vacuum suction apparatus II (3) comprises sleeve pipe II (3.1) and piston II (3.2), described sleeve pipe II (3.1) lower sides has filtering holes (3.7), in air-vent (2.4) and filtering holes (3.7), be respectively equipped with air filter film and air filtration filler, the aperture of air filter film and air filtration filler all is less than 0.22 μ m, described piston II (3.2) is located in sleeve pipe II (3.1), the bottom of described sleeve pipe II (3.1) is provided with suction attachment (3.4), the bottom of described suction attachment (3.4) is connected to suction pipe (3.5), described sleeve pipe I (1.1) and sleeve pipe II (3.1) bottom all can coordinate with the screw top of centrifuge tube (2).
2. a kind of external evoked patient self peripheral blood according to claim 1 produces the device of high concentration anti-inflammatory factors, it is characterized in that: the bottom of described sleeve pipe I (1.1) is protruded and is provided with female thread I (1.4), the bottom of described sleeve pipe II (3.1) is protruded and is provided with female thread II (3.3), the top end opening of described centrifuge tube (2) also is provided with external screw thread (2.1), and female thread I (1.4), female thread II (3.3) all can coordinate with external screw thread (2.1).
3. a kind of external evoked patient self peripheral blood according to claim 1 produces the device of high concentration anti-inflammatory factors, and it is characterized in that: the centre of described sleeve pipe I (1.1) also is provided with spacing ring (1.5).
4. a kind of external evoked patient self peripheral blood according to claim 1 produces the device of high concentration anti-inflammatory factors, and it is characterized in that: the outer race of described suction attachment (3.4) has germ tight filter (3.6).
5. a kind of external evoked patient self peripheral blood according to claim 1 produces the device of high concentration anti-inflammatory factors, and it is characterized in that: the bottom of described suction pipe (3.5) is bent upwards into hook-shaped.
6. a kind of external evoked patient self peripheral blood according to claim 1 produces the device of high concentration anti-inflammatory factors, and it is characterized in that: the bottom of described centrifuge tube (2) is placed with some glass beads (2.2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101212376A CN102631717B (en) | 2012-04-24 | 2012-04-24 | Device for in-vitro induction of self peripheral blood of patients to generate high-concentration anti-inflammatory factors |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101212376A CN102631717B (en) | 2012-04-24 | 2012-04-24 | Device for in-vitro induction of self peripheral blood of patients to generate high-concentration anti-inflammatory factors |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102631717A CN102631717A (en) | 2012-08-15 |
| CN102631717B true CN102631717B (en) | 2013-11-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101212376A Expired - Fee Related CN102631717B (en) | 2012-04-24 | 2012-04-24 | Device for in-vitro induction of self peripheral blood of patients to generate high-concentration anti-inflammatory factors |
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| Country | Link |
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| CN (1) | CN102631717B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039158A (en) * | 2015-07-31 | 2015-11-11 | 无锡阳光谷生命科技有限公司 | Simple small bioreactor |
| CN112375654B (en) * | 2020-11-20 | 2023-12-05 | 广州穗阳生物学研究有限公司 | Adipose-derived stem cell separation device and separation method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0655253A1 (en) * | 1993-10-26 | 1995-05-31 | Daiken Iki Co. Ltd. | Fluid aspiration-collection apparatus |
| CN2316955Y (en) * | 1997-11-03 | 1999-05-05 | 吴潭 | Vacuum blood collecting test-tube |
| WO2004101030A1 (en) * | 2003-05-15 | 2004-11-25 | Emilio Sacristan Rock | Air pressures-actuated driver for pneumatic ventricular assistance devices |
| CN1732028A (en) * | 2002-10-28 | 2006-02-08 | 史密夫及内修公开有限公司 | Apparatus for aspirating, irrigating and cleansing wounds |
| CN2785601Y (en) * | 2005-03-25 | 2006-06-07 | 吉安卡罗·马拉瓦西 | Autoblood recovering device |
| CN101352622A (en) * | 2008-09-17 | 2009-01-28 | 西安交通大学 | Blood separating device and separating method for filtering leucocyte |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE9904539D0 (en) * | 1999-12-10 | 1999-12-10 | Alphahelix Ab | Method and device for handling samples and reagents |
| CN201668712U (en) * | 2010-04-22 | 2010-12-15 | 李红新 | Test tube for blood test |
| CN202699684U (en) * | 2012-04-24 | 2013-01-30 | 祁小平 | Device for in-vitro induction of self peripheral blood of patients to generate high-concentration anti-inflammatory factors |
-
2012
- 2012-04-24 CN CN2012101212376A patent/CN102631717B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0655253A1 (en) * | 1993-10-26 | 1995-05-31 | Daiken Iki Co. Ltd. | Fluid aspiration-collection apparatus |
| CN2316955Y (en) * | 1997-11-03 | 1999-05-05 | 吴潭 | Vacuum blood collecting test-tube |
| CN1732028A (en) * | 2002-10-28 | 2006-02-08 | 史密夫及内修公开有限公司 | Apparatus for aspirating, irrigating and cleansing wounds |
| WO2004101030A1 (en) * | 2003-05-15 | 2004-11-25 | Emilio Sacristan Rock | Air pressures-actuated driver for pneumatic ventricular assistance devices |
| CN2785601Y (en) * | 2005-03-25 | 2006-06-07 | 吉安卡罗·马拉瓦西 | Autoblood recovering device |
| CN101352622A (en) * | 2008-09-17 | 2009-01-28 | 西安交通大学 | Blood separating device and separating method for filtering leucocyte |
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| CN102631717A (en) | 2012-08-15 |
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Address after: Room d312, 159 Chengjiang Middle Road, Jiangyin City, Wuxi City, Jiangsu Province Patentee after: Qi Xiaoping Patentee after: WUXI SUNLIGHT VALE LIFE TECHNOLOGIES Co.,Ltd. Address before: Room d312, 159 Chengjiang Middle Road, Jiangyin City, Wuxi City, Jiangsu Province Patentee before: Qi Xiaoping Patentee before: JIANGYIN BAISHENG BIOTECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20210629 Address after: 214200 room 109, building 4, No. 388, Yangxian West Road, Yicheng street, Yixing City, Wuxi City, Jiangsu Province Patentee after: Wuxi peierni Biotechnology Co.,Ltd. Address before: Room d312, 159 Chengjiang Middle Road, Jiangyin City, Wuxi City, Jiangsu Province Patentee before: Qi Xiaoping Patentee before: WUXI SUNLIGHT VALE LIFE TECHNOLOGIES Co.,Ltd. |
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Granted publication date: 20131127 |
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