CN102565405A - Method for immunological detection by combining acridinium ester labeling technology with general magnetic particles - Google Patents
Method for immunological detection by combining acridinium ester labeling technology with general magnetic particles Download PDFInfo
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- CN102565405A CN102565405A CN2011102432699A CN201110243269A CN102565405A CN 102565405 A CN102565405 A CN 102565405A CN 2011102432699 A CN2011102432699 A CN 2011102432699A CN 201110243269 A CN201110243269 A CN 201110243269A CN 102565405 A CN102565405 A CN 102565405A
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Abstract
The invention relates to the field of analyzing immunological determination, in particular to a method for immunological detection by combining an acridinium ester labeling technology with general magnetic particles and an immunological determination (quantitative/qualitative) kit which is prepared by utilizing the method. According to the method disclosed by the invention, substances, such as acridinium ester or acridine sulfonamide are used for labeling and detecting an antigen or an antibody, biotin is used for labeling and capturing the antigen or the antibody, and the immunological determination of biomolecules is carried out by combining the general (chain enzyme) avidin labeled magnetic particles. The method disclosed by the invention has the advantages of high sensitivity, wide detection range and capabilities of realizing the automation easily and having a wide application prospect in the field of the immunological detection.
Description
Technical field
The present invention relates to the immunologic assay analysis field, be specifically related to adopt the acridinium ester labelling technique to combine universal magnetic particle to carry out the method for immunology detection, and with the immunologic assay of this method preparation (quantitatively qualitative) kit.The present invention has combined acridinium ester chemistry luminescence technology, the general magnetic granule technology of biotin labeling technology and (chain enzyme) Avidin coupling.
Background technology
Common chemical luminous system has multiple at present; Topmost have: HRP-luminol system (or different luminol and derivant thereof), AP-spiral diamantane-1,2-two oxidative ethanes and derivant system thereof; Acridinium ester or acridine sulfonamide system, and electrochemiluminescsystem system etc.; Other is seen in documents and materials but glyoxaline compound, glucose oxidase, rosha acid esters, xanthine derivative or the like in addition of generally not promoting as yet used in commercialization.At present; The system that ripe relatively at present commercialization chemiluminescence immune assay reagent place adopts mainly is preceding four kinds; And in these four kinds of systems; Back relatively three kinds of low and poor stabilities of sensitivity of luminol (or different luminol and derivant thereof) system, the sensitivity of back three kinds of luminescence systems is all very high.
This wherein acridinium ester (or acridine sulfonamide) luminescence system for other systems, have its special advantage: at first, for luminous marker; The mark of acridinium ester or Aden's sulfonamides material is simple relatively; And label is luminous stable; The kit term of validity is long, and cost is relative also lower; And luminous is flash type, luminous concentrate fast and intensity big, the convenient fast detecting that realizes, the sensitivity of detection and precision are all very high, and simple relatively for the requirement of instrument, are convenient to realize full-automatic operation; Secondly, for luminescence system, acridinium ester (or acridine sulfonamide) also is the simplest with respect to other system, just can be directly luminous in alkalescence-superoxol, do not need reinforcing agent or catalyzer; Disturbing factor is few, and this sole is low, and signal to noise ratio (S/N ratio) is very high.These advantages that have based on the acridinium ester luminescence system; Develop acridinium ester class chemiluminescence immune assay reagents series box and necessary instrument; Say it is practicable from theoretical with practical significance, the present invention utilizes acridinium ester ((or acridine sulfonamide) these advantages of system is carried out the exploitation of immune reagent kit.
Biotin-(chain enzyme) Avidin system (biotin-(Strept) avidin system; BAS) the multilevel signal amplification that produces because (chain enzyme) Avidin molecule can combine the characteristic of four biotin molecules can significantly improve reaction sensitivity, and because the adaptability that biotin (chain enzyme) Avidin system is had: labeling method is simple and splendid for the resistance of varying environment; Once appearance; Promptly receive widespread use, but for a long time, what most literature and practical application were related all is the signal amplification of biological (chain enzyme) Avidin system; Basically all utilizing this system to improve the sensitivity of reaction system; Another big advantage of but having ignored this system: by the strong affinity between (chain enzyme) Avidin and the biotin, with (chain enzyme) Avidin coupling other such as polystyrene micropore plate, nano particle and other carrier mass that is fit to as universal support, just can connect any easily by biotinylated molecule; And then participate in further reacting; This application is not enlarged into purpose with signal, and provides a kind of general carrier, therefore has good application prospects in large-scale production and automation application field.The present invention promptly utilizes the ultra paramagnetic particle of (chain enzyme) Avidin coupling to form a kind of general carrier structure, and then is used to combine different biotinylated antigens or antibody, develops the kit that detects different biomacromolecules.
It is thing in recent years that ultra paramagnetic particulate is used for the immunoassay detection; As a kind of solid phase isolation methods, can improve the efficient of separation greatly compared to a traditional micro-pore plate type immunity type reaction, and be convenient to very much realize robotization; The magnetic particle has significantly increased effective binding site compared to the surface area that microwell plate improves greatly; Binding immunoassay is learned liquid phase reactor, can widen the range of linearity of immunology detection greatly, better adapts to clinical demand; And the good environmental resistance of magnetic particle makes it can adapt to the harsh reaction environment from acid to alkali, thereby makes reaction system have bigger pardon.But for a long time, people utilize the magnetic particle as solid phase carrier, the characteristics of just simply having utilized the magnetic particle to be easy to separate; The most application all is directly to be marked on the magnetic particle different molecular and then the participation immune response; Each project all need be with different antigens/antibody labeling on the magnetic particle, and for large-scale production, this will expend a lot of manpower and materials; Can have a strong impact on production efficiency; The present invention is intended to characteristic perfect adaptation that creationary versatility with biotin (chain enzyme) Avidin system and ultra paramagnetic particle system be easy to separate together, and (chain enzyme) Avidin with ultra paramagnetic particle coupling formation universal support, can be combined any biotinylated molecule; And utilize the high sensitivity and the high stability of acridinium ester detection system, develop different immunology detection kits.Novel part of the present invention has: (1) utilizes the ultra paramagnetic particle of (chain enzyme) Avidin coupling as general solid phase carrier, has both kept the stalling characteristic that is easy to of magnetic particle, (chain enzyme) versatility that Avidin is good of having withed a hook at the end; (2) select acridinium ester (or acridine sulfonamide) as luminescence system, have high sensitivity and high stability; (3) antigen of use monoclonal antibody or genetic recombination has improved the specificity of reaction greatly.
With the ultra paramagnetic particle of (chain enzyme) Avidin coupling as general solid phase carrier, with different biotinylated antigen/antibody molecules as capture agent, with the antigen/antibody molecule of acridinium ester (or acridine sulfonamide) mark as detectable, with NaOH-H
2O
2The exploitation of carrying out the immunology detection kit as substrate system is a kind of elder generation and then effective method, will promote the application and the development of chemiluminescence industry greatly.
Summary of the invention
The purpose of this invention is to provide a kind of ultra paramagnetic particle with (chain enzyme) Avidin coupling as general solid phase carrier; With different biotinylated antigen/antibody molecules as capture agent; With the antigen/antibody molecule of acridinium ester (or acridine sulfonamide) mark as detectable, with NaOH-H
2O
2Current techique platform as substrate system.
Another object of the present invention is to be the different immunology detection kit of foundational development with this technology platform.
Current techique platform according to the present invention comprises following technology:
1) technological with the ultra paramagnetic particle of Avidin coupling as general solid phase carrier;
2) with the labelling technique of the various reactive groups of the different capture antigen/antibody molecules of different biotin couplings;
3) cross the different labelling techniques that detect the various reactive groups of antigen/antibody molecule of different coupling agents coupling with different acridinium esters or acridine sulfonamide UNICOM;
4) with NaOH-H
2O
2For the basis, with acid and surfactant is the substrate system of reinforcing agent.
Concrete, (chain enzyme) of the present invention Avidin comprises: Avidin (Avidin), streptavidin (Streptavidin) and neutral Avidin (Neutravidin) and other can combine the class Avidin molecule of biotin molecule.
Ultra paramagnetic particle of the present invention be meant with the tri-iron tetroxide be kernel, surface parcel have such as amino (NH2-), carboxyl (COOH), hydroxyl (OH), sulfydryl (SH) or other be easy to the polymkeric substance of the reactive group of coupling, particle diameter is at the ultra paramagnetic particle of 5nm-5 μ m.
It is that any that core is done is the biotin derivative that purpose is modified generation with the coupling that biotin of the present invention is meant with
.
Acridinium ester of the present invention (or acridine sulfonamide) is meant that with
what be that core done is that purpose is modified acridinium ester (or the acridine sulfonamide) analog derivative that is produced to strengthen luminous intensity.
Coupling of the present invention or labelling technique are meant to be utilized common reactive group such as amino (NH2-), carboxyl (COOH), (OH), (SH) by different coupling agents such as dimethyl imines (EDC), glutaraldehyde, N, N '-dicyclohexylcarbodiimide (DCC), SATA, SMCC etc. carries out the method for intermolecular binding to sulfydryl to hydroxyl.
Substrate of the present invention is meant with NaOH-H
2O
2Carry out the luminous substrate system that is applicable to acridinium ester (or acridine sulfonamide) system of different proportioning combinations for the basis.
Immunology detection kit of the present invention is meant the detection kit based on immunological response that can be used for detecting medical science pathogenic microorganism, clinical disease mark, environment Molecular Detection, food inspection and other similar application of utilizing the present technique platform development.
Concrete mentioned reagent box can comprise calibration object (or positive and negative reference substance), biotinylated antigen/antibody, and (chain enzyme) Avidin encapsulates general magnetic particle, acridinium ester labelled antigen/antibody, lavation buffer solution, chemical luminous substrate etc.Wherein, the raw material of said calibration object (or positive and negative reference substance) is a standard level, and purity is not less than 90%; Antigen is the genetic engineering recombinant antigen, and antibody is monoclonal antibody, and purity is not less than 95% (SDS-PAGE method); Lavation buffer solution is a phosphate buffer; Chemical luminous substrate is NaOH-H
2O
2
Embodiment
Below come further explaination the present invention through specific embodiment, but these embodiment are not as restrictive condition of the present invention.
Embodiment 1
High sensitivity quantitation detects the acridinium ester chemistry luminous detection kit preparation method of alpha-fetoprotein in the serum
The preparation method of present embodiment may further comprise the steps:
The preparation of A, general magnetic particle:
The preparation of magnetic particle lavation buffer solution: with distilled water and 2-(N-quinoline) ethyl sulfonic acid (MES) secure ph is 4.7-5.0; Concentration is the MES damping fluid of 50mmol/ml; Add Tween-20 to final concentration be 0.15%; The Proclin-300 final concentration is that 2-8 ℃ of preservation is subsequent use after 0.1%, the 0.22 μ um filtering with microporous membrane degerming, and the term of validity is three months.
The preparation of mark damping fluid: use distilled water; Boric acid and borax preparation pH are 8.3-8.7; Final concentration is the borate buffer of 50mmol/ml, and adding Tween-20 is 0.15% to final concentration, and the Proclin-300 final concentration is 0.1%; 0.22 2-8 ℃ of preservation is subsequent use after the degerming of μ um filtering with microporous membrane, the term of validity is three months.
The preparation of working buffer liquid: use distilled water, sodium dihydrogen phosphate and sodium hydrogen phosphate preparation pH are 7.0-7.4, and final concentration is the phosphate buffer of 50mM, adds PVP; BSA, Tween-20, sucrose, Proclin-300; Final concentration is respectively 1%, 1%, and 0.1%, 3%; 2-8 ℃ of preservation be subsequent use after 0.2%, the 0.22 μ um filtering with microporous membrane degerming, is valid for one year.
The preparation method of the general magnetic particle of streptavidinization is: use magnetic particle lavation buffer solution damping fluid washing magnetic particle (0.5-5um); Add EDC and make the two final concentration be 50mmol with NHS, room temperature reaction 30-60 minute, wash 3 times after the adding Streptavidin to make the mol ratio of streptavidin and magnetic particle be 5: 1; Room temperature reaction 3 hours; Add the 50mM PBS room temperature that contains 0.5%BSA and sealed 30 minutes, wash 5 times, use working buffer liquid redissolution magnetic particle (1mg magnetic particle redissolves with 20ml working buffer liquid); Be distributed into 5ml/ and prop up, 2-8 ℃ of preservation is subsequent use.
The preparation of B, biotinylated antibody and acridinium ester labelled antibody hybrid working liquid
The preparation method of the anti-AFP capture antibody of biotinylation is: will resist the AFP capture antibody to 2-8 ℃ of dialysed overnight of 0.02M PBS; Adjustment concentration is 2mg/ml; Preparatory activation biotin is used the pure water dissolving; Final concentration is 50mmol/ml, and with 10-50: 1 molecule ratio adds the biotin solution of aequum in antibody-solutions, room temperature reaction 30 minutes; Change over to afterwards molecule hold back diameter be in the bag filter of 4000-8000 to 2-8 ℃ of dialysed overnight of 0.02M PBS, add equal-volume glycerine after-20 ℃ of preservations subsequent use.The preparation method that acridinium ester (or acridine sulfonamide) mark AFP detects antibody is: will resist AFP to detect antibody to 2-8 ℃ of dialysed overnight of 0.05M MES; Adjustment concentration is 2mg/ml; Preparatory activation acridinium ester (or acridine sulfonamide) is used the pure water dissolving; Final concentration is 10mmol/ml, and with 10-50: 1 molecule ratio adds acridinium ester (or the acridine sulfonamide) solution of aequum, room temperature reaction 30 minutes in antibody-solutions; Change over to afterwards molecule hold back diameter be in the bag filter of 4000-8000 to 2-8 ℃ of dialysed overnight of 0.05M MES, add behind the equal-volume glycerine-20 ℃ and keep in Dark Place subsequent use.
The mixed diluting of biotinylated antibody and acridinium ester labelled antibody hybrid working liquid: with the ratio of biotinylation capture antibody with 1/3000-6000; Acridinium ester marker detection antibody is with the ratio of 1/1000-2000; Mixed diluting is in working buffer liquid; Add the HAMA antibody inhibition, final concentration is 2-5%, is distributed into 10ml/ with brown bottle and props up 2-8 ℃ and keep in Dark Place subsequent use.
The preparation of C, AFP calibration solution
Get the pure article of AFP, after the use AFP national standard article definite value, be diluted to 5ng/ml and 400ng/ml with cow's serum (containing 0.1%Proclin-300), be distributed into 1ml/ and prop up ,-20 ℃ of preservations are subsequent use.
The preparation of D, concentrated cleaning solution
Use distilled water, sodium dihydrogen phosphate and sodium hydrogen phosphate preparation pH is 7.0-7.4, and final concentration is the phosphate buffer of 1M, and adding the NaCL final concentration is 300mM, and adding the Tween-20 final concentration is 5%, and being distributed into 50ml/, to prop up 2-8 ℃ of preservation subsequent use.
The preparation of F, substrate solution
A liquid: be mixed with the dilute nitric acid solution of 0.01-0.03M with distilled water and nitric acid, adding the hydrogen peroxide final concentration is 0.1-0.5%, and being distributed into 20ml/ with brown bottle, to prop up 2-8 ℃ of preservation subsequent use.
B liquid: be mixed with the dilute alkaline soln of 0.02-0.06M with distilled water and NaOH, adding the surfactant SDS final concentration is 0.5-2%, and being distributed into 20ml/, to prop up 2-8 ℃ of preservation subsequent use.
The assembling of G, kit
With above-mentioned magnetic particle working fluid, biotinylated antibody and acridinium ester labelled antibody hybrid working liquid, AFP calibration solution, lavation buffer solution and substrate solution are packed in the kit, and encapsulation is gone into 2-8 ℃ of freezer after the assay was approved and preserved.
Through detecting, the Key Performance Indicator of this kit is:
Sensitivity: 0.5ng/ml,
Accuracy in batch:＜10%,
Accuracy between batch:＜15%
Accuracy: the national standard article detect deviation in ± 10%.
Clinical accordance: use 526 parts of clinical samples, test comparison, carry out correlation analysis, related coefficient＞0.975 with import AFP chemical illuminating reagent.
Stability: be no less than 12 months, the Kaifeng rear stability is no less than 28 days.
Embodiment 2
The acridinium ester of HIV-1+2 antibody and P24 antigen chemistry luminous detection kit preparation method in the joint-detection blood
The preparation method of present embodiment may further comprise the steps:
The preparation of A, general magnetic particle:
The preparation of magnetic particle lavation buffer solution: with embodiment 1.
The preparation of mark damping fluid: with embodiment 1.
The preparation of working buffer liquid: with embodiment 1.
The preparation method of the general magnetic particle of neutral Avidinization is: use magnetic particle lavation buffer solution damping fluid washing magnetic particle (0.5-5um); Add EDC and make the two final concentration be 50mmol with NHS, room temperature reaction 30-60 minute, wash 3 times after the neutral Avidin of adding to make the mol ratio of neutral Avidin and magnetic particle be 3-5: 1; Room temperature reaction 3 hours; Add the 50mM PBS room temperature that contains 0.5%BSA and sealed 30 minutes, wash 5 times, use working buffer liquid redissolution magnetic particle (1mg magnetic particle redissolves with 20ml working buffer liquid); Be distributed into 5ml/ and prop up, 2-8 ℃ of preservation is subsequent use.
The preparation of B, biotinylated antigen/antibody and acridinium ester labelled antigen/antibody hybrid working liquid
The preparation method of biotinylation HIV 1+2 reorganization capture antigen is: HIV 1+2 is recombinated capture antigen to 2-8 ℃ of dialysed overnight of 0.02M PBS (containing 0.1%SDS); Adjustment concentration is 5mg/ml; Preparatory activation biotin is used the pure water dissolving; Final concentration is 50mmol/ml, and with 10-50: 1 molecule ratio adds the biotin solution of aequum in antigenic solution, room temperature reaction 30 minutes; Change over to afterwards molecule hold back diameter be in the bag filter of 4000-8000 to 2-8 ℃ of dialysed overnight of 0.02M PBS (containing 0.1%SDS), it is subsequent use with protected protein (0.5%BSA) 2-8 ℃ of preservation afterwards to add equal-volume glycerine.
The preparation method of biotinylation HIV p24 capture antibody is: with the p24 capture antibody to 2-8 ℃ of dialysed overnight of 0.02M PBS; Adjustment concentration is 2mg/ml; Preparatory activation biotin is used the pure water dissolving; Final concentration is 50mmol/ml, and with 10-50: 1 molecule ratio adds the biotin solution of aequum in antibody-solutions, room temperature reaction 30 minutes; Change over to afterwards molecule hold back diameter be in the bag filter of 4000-8000 to 2-8 ℃ of dialysed overnight of 0.02M PBS, add equal-volume glycerine after-20 ℃ of preservations subsequent use.The preparation method that acridinium ester (or acridine sulfonamide) mark HIV 1+2 reorganization detects antigen is: HIV 1+2 is detected antigen to 2-8 ℃ of dialysed overnight of 0.02M PBS (containing 0.1%SDS); Adjustment concentration is 5mg/ml; Preparatory activation acridinium ester (or acridine sulfonamide) is used the pure water dissolving; Final concentration is 10mmol/ml; With 10-50: 1 molecule ratio adds acridinium ester (or the acridine sulfonamide) solution of aequum in antigenic solution; Room temperature reaction 60 minutes, change over to afterwards molecule hold back diameter be in the bag filter of 4000-8000 to 2-8 ℃ of dialysed overnight of 0.02M PBS (containing 0.1%SDS), add equal-volume glycerine and protected protein (0.5%BSA) afterwards 2-8 ℃ keep in Dark Place subsequent use.
The preparation method that acridinium ester (or acridine sulfonamide) mark HIV p24 detects antibody is: with HIV p24 antibody to 0.05M MES2-8 ℃ dialysed overnight; Adjustment concentration is 2mg/ml; Preparatory activation acridinium ester (or acridine sulfonamide) is used the pure water dissolving; Final concentration is 10mmol/ml, and with 10-50: 1 molecule ratio adds acridinium ester (or the acridine sulfonamide) solution of aequum, room temperature reaction 30 minutes in antibody-solutions; Change over to afterwards molecule hold back diameter be in the bag filter of 4000-8000 to 0.05M MES2-8 ℃ dialysed overnight, add behind the equal-volume glycerine-20 ℃ and keep in Dark Place subsequent use.
The mixed diluting of biotinylated antigen/antibody and acridinium ester labelled antigen/antibody hybrid working liquid: with the ratio of biotinylation HIV 1+2 reorganization capture antigen with 1/3000-6000; Biotinylation HIV p24 capture antibody is with the ratio of 1/2000-4000; Acridinium ester mark HIV 1+2 reorganization detects the ratio of antigen with 1/2000-5000; Acridinium ester mark HIV p24 detection antibody is diluted in the working buffer liquid with the mixed of 1/1500-3000; Add the HAMA antibody inhibition, final concentration is 1-3%, is distributed into 10ml/ with brown bottle and props up 2-8 ℃ and keep in Dark Place subsequent use.
The preparation of C, HIV quality-control product
Get positive inactivated serum of HIV and the pure article of reorganization p24 antigen, with reference to national HIV reference serum dish, be diluted to two Quality Controls of positive and negative with cow's serum (containing 0.1%Proclin-300), be distributed into 1ml/ and prop up ,-20 ℃ of preservations are subsequent use.
The preparation of D, concentrated cleaning solution: with embodiment 1.
The preparation of F, substrate solution:
A liquid: be mixed with the dilute nitric acid solution of 0.01-0.03M with distilled water and nitric acid, adding the urea peroxide final concentration is 0.2-1.0%, and being distributed into 20ml/ with brown bottle, to prop up 2-8 ℃ of preservation subsequent use.
B liquid: be mixed with the dilute alkaline soln of 0.02-0.06M with distilled water and NaOH, adding surfactant CTAC final concentration is 0.1-0.5%, and being distributed into 20ml/, to prop up 2-8 ℃ of preservation subsequent use.
The assembling of G, kit
With above-mentioned magnetic particle working fluid, biotinylated antigen/antibody and acridinium ester labelled antigen/antibody hybrid working liquid, HIV quality-control product, concentrated cleaning solution and substrate solution are packed in the kit, and encapsulation is gone into 2-8 ℃ of freezer after the assay was approved and preserved.
Through detecting, the Key Performance Indicator of this kit is:
Sensitivity: national reference serum dish detects ratio and is (4/6),
Accuracy in batch:＜10%,
Accuracy between batch:＜15%
Accuracy: the complete conformance with standard requirement of national reference serum dish, positive (20/20), negative (20/20).
Clinical accordance: use 1482 parts of clinical samples, test comparison with import HIV1+2/p24 joint inspection reagent, positive coincidence rate 100%, negative match-rate (98%), Kappa consistency analysis, Kappa coefficient＞0.9.
Stability: be no less than 12 months, the Kaifeng rear stability is no less than 28 days.
Claims (9)
1. one kind combines universal magnetic particle to carry out the method for immunology detection with the acridinium ester labelling technique, is characterised in that said method has merged following technical method:
1) technological with the ultra paramagnetic particle of Avidin coupling as general solid phase carrier;
2) with the labelling technique of the various reactive groups of the different capture antigen/antibody molecules of different biotin couplings;
3) cross the different labelling techniques that detect the various reactive groups of antigen/antibody molecule of different coupling agents coupling with different acridinium esters or acridine sulfonamide UNICOM;
4) with NaOH-H
2O
2For the basis, with acid and surfactant is the substrate system of reinforcing agent.
2. according to claim 1ly a kind ofly combine universal magnetic particle to carry out the method for immunology detection, be characterised in that with the acridinium ester labelling technique:
1) said " Avidin " is selected from the class Avidin molecule that Avidin, streptavidin, neutral Avidin maybe can combine biotin molecule;
2) said " ultra paramagnetic particle " is to be the polymkeric substance that kernel, surface parcel have various reactive groups with the tri-iron tetroxide, and particle diameter is at the ultra paramagnetic particle of 50nm-5 μ m.
3. according to claim 1ly a kind ofly combine universal magnetic particle to carry out the method for immunology detection, be characterised in that it is that any that core is done is the biotin derivative that purpose is modified generation with the coupling that the biotin of selecting for use is meant with
with the acridinium ester labelling technique.
4. describedly a kind ofly combine universal magnetic particle to carry out the method for immunology detection according to claim 1～3 is arbitrary, be characterised in that said various reactive groups are amino, carboxyl, hydroxyl, sulfydryl with the acridinium ester labelling technique; Said different coupling agents is dimethyl imines, glutaraldehyde, N, N '-dicyclohexylcarbodiimide, SATA, SMCC.
5. according to claim 1ly a kind ofly combine universal magnetic particle to carry out the method for immunology detection, be characterised in that said substrate system is with NaOH-H with the acridinium ester labelling technique
2O
2Carry out the luminous substrate system that is applicable to acridinium ester or acridine sulfonamide system of different proportionings combinations as reinforcing agent for the basis, with acid and surfactant.
6. describedly a kind ofly combine universal magnetic particle to carry out the method for immunology detection according to claim 1 or 5, be characterised in that said acid is nitric acid, sulfuric acid, hydrochloric acid with the acridinium ester labelling technique; Said surfactant is SDS, Tween, Triton-X, Brij, CTAC.
7. alpha-fetoprotein chemiluminescence detection kit that utilizes each described method of claim 1～6 to detect is characterised in that said kit comprises:
The ultra paramagnetic particle of streptavidin coupling;
Capture antibody, said capture antibody is for combining the biotinylation alpha-fetoprotein monoclonal antibody of biotin labeling technology mark;
Detect antibody, said detection antibody is the acridinium ester-alpha-fetoprotein monoclonal antibody of acridinium ester labelling technique mark; And
By A liquid HNO
3-H
2O
2, the substrate system formed of B liquid NaOH-SDS.
8. chemical luminescence reagent kit that utilizes HIV 1+2 and p24 antibody in the joint-detection blood that each described method of claim 1～6 detects is characterised in that said kit comprises:
The ultra paramagnetic particle of neutral Avidin coupling;
Biotinylation HIV 1+2 recombinant antigen and biotinylation HIV p24 antibody in conjunction with biotin labeling technology mark;
The acridinium ester of acridinium ester labelling technique mark-HIV 1+2 recombinant antigen and acridinium ester-HIV p24 antibody; And
By A liquid HNO
3-H
2O
2, the substrate system formed of B liquid NaOH-SDS.
9. each described method of claim 1～6 is in the application in pathogenic microorganism detection, disease marker detection, drugs detection, environmental monitoring, food safety detection field.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000019201A1 (en) * | 1998-09-25 | 2000-04-06 | Quest Diagnostics Investments Incorporated | Biological assay compositions containing non-interfering, foam-inhibiting and foam-collapsing agents |
| US20030027234A1 (en) * | 2001-07-30 | 2003-02-06 | Pandian Murugan R. | Methods for detecting Down's syndrome |
| CN1542449A (en) * | 2003-04-30 | 2004-11-03 | �����ɷ� | Nuclear/shell type superparamagnetism composite particulate, preparation method and application thereof |
| CN1952659A (en) * | 2005-10-19 | 2007-04-25 | 陕西西大北美基因股份有限公司 | An avidin/ streptavidin magnetic complex particle and the process thereof and purpose thereof |
| CN101038255A (en) * | 2007-04-06 | 2007-09-19 | 福州大学 | Capillary electrophoresis chemiluminescence detector of acridiniumester, acridine sulfonamide and marker thereof, and method thereof |
| CN101377503A (en) * | 2008-03-25 | 2009-03-04 | 北京科美东雅生物技术有限公司 | Hepatitis b virus preS2 antigen chemiluminescence immune analysis determination reagent kit and preparing method thereof |
| CN101533028A (en) * | 2008-03-14 | 2009-09-16 | 北京科美东雅生物技术有限公司 | Chemoluminescent immunoassay kit of hyaluronic acid and preparation method thereof |
| CN101539576A (en) * | 2008-03-21 | 2009-09-23 | 北京科美东雅生物技术有限公司 | Hepatitis B virus pre S1 antigen chemiluminscence immunoassay kit and preparation method thereof |
| CN101566631A (en) * | 2008-04-24 | 2009-10-28 | 北京科美东雅生物技术有限公司 | Magnetic immunochromatographic test strip for combined detection of HIV-1+2 antibody and P24 antigen and preparation method thereof |
| CN101609090A (en) * | 2008-06-19 | 2009-12-23 | 北京华科泰生物技术有限公司 | A kind of employing acridinium ester labelled antigen or antibody analysis method |
-
2011
- 2011-08-24 CN CN2011102432699A patent/CN102565405A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000019201A1 (en) * | 1998-09-25 | 2000-04-06 | Quest Diagnostics Investments Incorporated | Biological assay compositions containing non-interfering, foam-inhibiting and foam-collapsing agents |
| US20030027234A1 (en) * | 2001-07-30 | 2003-02-06 | Pandian Murugan R. | Methods for detecting Down's syndrome |
| CN1542449A (en) * | 2003-04-30 | 2004-11-03 | �����ɷ� | Nuclear/shell type superparamagnetism composite particulate, preparation method and application thereof |
| CN1952659A (en) * | 2005-10-19 | 2007-04-25 | 陕西西大北美基因股份有限公司 | An avidin/ streptavidin magnetic complex particle and the process thereof and purpose thereof |
| CN101038255A (en) * | 2007-04-06 | 2007-09-19 | 福州大学 | Capillary electrophoresis chemiluminescence detector of acridiniumester, acridine sulfonamide and marker thereof, and method thereof |
| CN101533028A (en) * | 2008-03-14 | 2009-09-16 | 北京科美东雅生物技术有限公司 | Chemoluminescent immunoassay kit of hyaluronic acid and preparation method thereof |
| CN101539576A (en) * | 2008-03-21 | 2009-09-23 | 北京科美东雅生物技术有限公司 | Hepatitis B virus pre S1 antigen chemiluminscence immunoassay kit and preparation method thereof |
| CN101377503A (en) * | 2008-03-25 | 2009-03-04 | 北京科美东雅生物技术有限公司 | Hepatitis b virus preS2 antigen chemiluminescence immune analysis determination reagent kit and preparing method thereof |
| CN101566631A (en) * | 2008-04-24 | 2009-10-28 | 北京科美东雅生物技术有限公司 | Magnetic immunochromatographic test strip for combined detection of HIV-1+2 antibody and P24 antigen and preparation method thereof |
| CN101609090A (en) * | 2008-06-19 | 2009-12-23 | 北京华科泰生物技术有限公司 | A kind of employing acridinium ester labelled antigen or antibody analysis method |
Non-Patent Citations (4)
| Title |
|---|
| C.DODEIGNE等: "Chemiluminescence as diagnostic tool. A review", 《TALANTA》 * |
| F.JAVIER BAGAZGOITIA等: "Effect of surfactants on the intensity of chemiluminescence emission from acridinium ester labelled proteins.", 《JOURNAL OF BIOLUMINESCENCE AND CHMEILUMINESCENCE》 * |
| LIXIA ZHAO等: "Chemiluminescence immunoassay", 《TRENDS IN ANALYTICAL CHEMISTRY》 * |
| 唐秋艳: "《免疫诊断试剂实用技术》", 31 August 2009 * |
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