CN102559476B - A kind of microorganism seperation culture device - Google Patents
A kind of microorganism seperation culture device Download PDFInfo
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- CN102559476B CN102559476B CN201010612624.0A CN201010612624A CN102559476B CN 102559476 B CN102559476 B CN 102559476B CN 201010612624 A CN201010612624 A CN 201010612624A CN 102559476 B CN102559476 B CN 102559476B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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Abstract
The invention discloses a kind of microorganism seperation culture device, comprise the upper cover and one that are made by transparent high temperature resistant material for placing the lower cover of substratum, the culture vessel of described upper cover and superimposed rear this culture apparatus of formation of lower cover, cover on described and offer for direct application of sample module of carrying out application of sample on substratum, one obtains single bacterium colony inoculation cut module for carrying out cut on substratum is at least provided with in described culture vessel, described application of sample module be opened in described on the loading slot of at least one that covers or the well of at least one, or the loading slot of any number and the combination of well.Microorganism seperation culture device of the present invention can when without the need to opening culture dish upper cover, simple and effective sample is inoculated into solid culture primary surface, and inoculation cut module can be utilized, manually, semi-automatic or automatic mode by sample drawing cloth to be seeded to media surface, to obtain single bacterium colony.
Description
Technical field
The present invention relates to the separation and Culture of microorganism and the association area of qualification, be specifically related to a kind of microorganism seperation culture device.
Background technology
Test at clinical micro-biopsy, be correlated with teaching and scientific research experiment, Food microbe testing etc. of microorganism relate in the field of Isolation and identification of microorganism, because detected sample mostly is the mixture of multiple different strain or different subtype microorganism, to identify accurately or subsequent experimental sample, need to obtain purer microbe colony, therefore, the single bacterium colony of microorganism is separated and just seems particularly important.
In actual mechanical process, the separation of bacterium colony is usually by technician's manual operations.That is: aseptically, technician opens culture dish upper cover, the line in the first direction on culture medium flat plate surface with metal or plastics transfering loop, formed on the first cut band and produce multiple cut, then transfering loop is sterilized afterwards or used new ring to stride across described first cut band and rule at different directions, produce the second cut band, same to aforementioned operation, generally also need to mark the 3rd even the 4th cut band.Generally in operation, usually adopt flame heating or electric heater heating, transfering loop, sampling receptacle mouth etc. are carried out disinfection.Then at the edge of agar plate used, solid medium or directly in atmosphere transfering loop is cooled fast.
Such work repeatability is strong, has higher requirement to the figure cloth technical ability of technician and skill level.For the inoculation of a large amount of samples, this work is dry as dust, and a large amount of repetitions easily causes mishandle, brings to diagnosis, qualification the consequence that error is even serious.
Adopt plastic cement band scratcher and wherein said automatic scoring devices in patent WO2005/071055 and CN1918280A, efficiently solve some drawback of artificial inoculation microbiological specimens.Its specific operation process is: open culture dish upper cover, to treat that the pending sample of figure cloth is divided into a fine rule in media surface by imbibition instrument, again by plastic strip scratcher under the traction of CD-ROM drive motor, the sample of media surface is scratched, again upper cover is restored afterwards, scratcher and liquid-transfering sucker are recovered, and re-use or discard after sterilization.
But above scheme still cannot solve the operation of opening upper cover in microbial inoculant (application of sample and Tu Bu) process.And this operation adds the risk of crossed contamination virtually.The time that upper cover is lifted is longer, and the risk of crossed contamination is larger.During for isolation identification pathogenic micro-organism, such operation also adds the risk that operator infect relative disease virtually.
On the other hand, scheme described in patent CN1918280A and preferred embodiment thereof more lay particular emphasis on inoculation and the figure cloth of liquid sample, and in actually operating, liquid sample is only sub-fraction, and the inoculation for on-liquid sample does not provide effective solution.
Summary of the invention
In order to overcome deficiency of the prior art, the object of the present invention is to provide a kind of microorganism seperation culture device, this device without the need to opening culture dish upper cover, effectively prevent crossed contamination when carrying out the cultivation of microorganism.
In order to solve the problems of the technologies described above, realize above-mentioned purpose, the present invention is achieved through the following technical solutions:
A kind of microorganism seperation culture device, comprise the upper cover and one that are made by transparent high temperature resistant material for placing the lower cover of substratum, the culture vessel of described upper cover and superimposed rear this culture apparatus of formation of lower cover, covering on described and offer for direct application of sample module of carrying out application of sample on substratum, in described culture vessel, being at least provided with one obtains single bacterium colony inoculation cut module for carrying out cut on substratum.
Further, described application of sample module be opened in described on the loading slot of at least one that covers or the well of at least one, or the loading slot of any number and the combination of well.
Preferably, described upper cover becomes disc with lower cover, forms a cylindrical culture vessel.
Preferably, the top of described inoculation cut module is fixed on the interior capping of described upper cover.
Preferably, the central position of described upper cover is provided with a rotary actuation bar, and the central position of described lower cover is provided with the directed seat of a driving stem, described upper cover and lower cover superimposed time, described rotary actuation bar and the directed seat of driving stem are just in time installed and are combined together.
Further, the side of described inoculation cut module is fixed on the side of described rotary actuation bar.
Preferably, described upper cover and lower cover rectangularity lid, form the culture vessel of a rectangular parallelepiped, described upper cover also offers guide path, be provided with moving track in the container of described lower cover, the top of described inoculation cut module is provided with driving stem, and side is provided with orienting lug, described orienting lug is erected on described moving track, and described driving stem is arranged in described guide path.
Further, described moving track comprises the smooth section, rising ramp section and the positioning section that connect successively.
Further, described positioning section is in depression structure or bulge-structure.
Further, the contact surface of the substratum in described inoculation cut module and described lower cover is an one-piece construction or is spaced from each other structure in a row.
Compared with prior art, microorganism seperation culture device of the present invention can when without the need to opening culture dish upper cover, simple and effective sample is inoculated into solid culture primary surface, and inoculation cut module can be utilized, manually, semi-automatic or automatic mode by sample drawing cloth to be seeded to media surface, to obtain single bacterium colony.
Above-mentioned explanation is only the general introduction of technical solution of the present invention, in order to better understand technique means of the present invention, and can be implemented according to the content of specification sheets, coordinates accompanying drawing to be described in detail as follows below with preferred embodiment of the present invention.The specific embodiment of the present invention is provided in detail by following examples and accompanying drawing thereof.
Accompanying drawing explanation
Below in conjunction with drawings and embodiments, the present invention is further detailed explanation.
Fig. 1 is the lower cover structure schematic diagram of the first embodiment of the present invention.
Fig. 2 is the structural representation at a visual angle of the upper cover of the first embodiment of the present invention.
Fig. 3 is the structural representation at another visual angle of the upper cover of the first embodiment of the present invention.
Fig. 4 is the lower cover structure schematic diagram of the second embodiment of the present invention.
Fig. 5 is the structural representation at a visual angle of the upper cover of the second embodiment of the present invention.
Fig. 6 is the sectional view of the second embodiment of the present invention.
Fig. 7 is the structural representation of the inoculation cut module of the second embodiment of the present invention.
Number in the figure illustrates: 1, upper cover, 101, application of sample module, and 102 rotary actuation bars, 103, guide path, 2, lower cover, 201, the directed seat of driving stem, 202, moving track, 2021, smooth section, 2022, rising ramp section, 2023, positioning section, 3, cut module is inoculated, 301, driving stem, 302, orienting lug, 303, contact surface.
Embodiment
Embodiment 1:
Shown in Fig. 1, Fig. 2, Fig. 3, a kind of microorganism seperation culture device, comprise the upper cover 1 and one that are made by transparent high temperature resistant material for placing the lower cover 2 of substratum, the culture vessel of described upper cover 1 and superimposed rear this culture apparatus of formation of lower cover 2, described upper cover 1 offering one for direct application of sample module 101 of carrying out application of sample on substratum, in described culture vessel, being at least provided with one obtains single bacterium colony inoculation cut module 3 for carrying out cut on substratum.
Further, described application of sample module 101 is for being opened in the loading slot of at least one on described upper cover 1 or the well of at least one, or the loading slot of any number and the combination of well.
Preferably, described upper cover 1 and lower cover 2 one-tenth disc, form a cylindrical culture vessel.
Preferably, the top of described inoculation cut module 3 is fixed on the interior capping of described upper cover 1.
Preferably, the central position of described upper cover 1 is provided with a rotary actuation bar 102, the central position of described lower cover 2 is provided with the directed seat 201 of a driving stem, described upper cover 1 and lower cover 2 superimposed time, described rotary actuation bar 102 and the directed seat 201 of driving stem are just in time installed and are combined together.
Further, the side of described inoculation cut module 3 is fixed on the side of described rotary actuation bar 102.
Operating process of the present utility model is as follows:
Transfering loop or suction pipette head are by loading slot 101 and along loading slot 101, and the media surface inoculation in lower cover 2 or application of sample, the substratum thus in lower cover 2 produces along radial direction first cut band; Afterwards, external force drives inoculation cut module 3 to rotate around the center of circle in media surface, thus along forming the second cut band perpendicular to radial direction by rotary actuation bar 102 or upper cover 1 main body directly or indirectly.
Embodiment 2:
Shown in Fig. 4, Fig. 5, Fig. 6, a kind of microorganism seperation culture device, comprise the upper cover 1 and one that are made by transparent high temperature resistant material for placing the lower cover 2 of substratum, the culture vessel of described upper cover 1 and superimposed rear this culture apparatus of formation of lower cover 2, described upper cover 1 offering one for direct application of sample module 101 of carrying out application of sample on substratum, in described culture vessel, being at least provided with one obtains single bacterium colony inoculation cut module 3 for carrying out cut on substratum.
Further, described application of sample module 101 is for being opened in the loading slot of at least one on described upper cover 1 or the well of at least one, or the loading slot of any number and the combination of well.
Further, described upper cover 1 and lower cover 2 rectangularity lid, form the culture vessel of a rectangular parallelepiped, described upper cover 1 also offers guide path 103, be provided with moving track 202 in the container of described lower cover 2, the top of described inoculation cut module 3 is provided with driving stem 301, side is provided with orienting lug 302, described orienting lug 302 is erected on described moving track 202, and described driving stem 301 is arranged in described guide path 103.
Further, described moving track 202 comprises a smooth section 2021.
Further described moving track 202 also comprises the rising ramp section 2022 be connected with described smooth section 2021.
Further, described moving track 202 also comprises the positioning section 2023 be connected with described rising ramp section 2022.
Further, described positioning section 2023 is in depression structure or bulge-structure.
Further, shown in Figure 7, described inoculation cut module 3 is an one-piece construction with the contact surface 303 of the substratum in described lower cover 2 or is spaced from each other structure in a row.
Operating process of the present utility model is as follows:
Transfering loop or suction pipette head are by loading slot 101 and along loading slot 101, and the media surface inoculation in lower cover 2 or application of sample, longitudinally produce the first cut band at substratum thus; Drive driving stem 301 by external force afterwards, drive inoculation cut module 3 to do transverse movement along moving track 202, laterally forming the second cut band thus.When inoculating cut module 3 and moving to the end close to lower cover 2, inoculation cut module 3 rises along rising ramp section 2022, no longer directly contacts with media surface.To most end place, inoculation cut module 3 is fixed on positioning section 2023 place, can not slide easily, and its bottom surface and media surface has certain distance, thus no longer affects cultivation and the observation of microorganism.
Above-described embodiment, just in order to technical conceive of the present invention and feature are described, its objective is and is one of ordinary skilled in the art can be understood content of the present invention and implement according to this, can not limit the scope of the invention with this.The change of every equivalence done by the essence of content of the present invention or modification, all should be encompassed in protection scope of the present invention.
Claims (4)
1. a microorganism seperation culture device, comprise the upper cover (1) that made by transparent high temperature resistant material and the lower cover (2) for placing substratum, the culture vessel of described upper cover (1) and superimposed rear this culture apparatus of formation of lower cover (2), it is characterized in that: described upper cover (1) offers for direct application of sample module (101) of carrying out application of sample on substratum, being at least provided with one in described culture vessel for carrying out cut to obtain the inoculation cut module (3) of single bacterium colony on substratum; Described application of sample module (101) is for being opened in the loading slot of at least one on described upper cover (1) or the well of at least one, or the loading slot of any number and the combination of well; Described upper cover (1) becomes disc with lower cover (2), forms a cylindrical culture vessel; The top of described inoculation cut module (3) is fixed on the interior capping of described upper cover (1), the central position of described upper cover (1) is provided with a rotary actuation bar (102), the central position of described lower cover (2) is provided with the directed seat (201) of a driving stem, described upper cover (1) and lower cover (2) superimposed time, described rotary actuation bar (102) and the directed seat (201) of driving stem are just in time installed and are combined together, and the side of described inoculation cut module (3) is fixed on the side of described rotary actuation bar (102).
2. a microorganism seperation culture device, comprise the upper cover (1) that made by transparent high temperature resistant material and the lower cover (2) for placing substratum, the culture vessel of described upper cover (1) and superimposed rear this culture apparatus of formation of lower cover (2), it is characterized in that: described upper cover (1) offers for direct application of sample module (101) of carrying out application of sample on substratum, being at least provided with one in described culture vessel for carrying out cut to obtain the inoculation cut module (3) of single bacterium colony on substratum, described application of sample module (101) is for being opened in the loading slot of at least one on described upper cover (1) or the well of at least one, or the loading slot of any number and the combination of well, described upper cover (1) and described lower cover (2) rectangularity lid, form the culture vessel of a rectangular parallelepiped, described upper cover (1) also offers guide path (103), moving track (202) is provided with in the container of described lower cover (2), the top of described inoculation cut module (3) is provided with driving stem (301), side is provided with orienting lug (302), described orienting lug (302) is erected on described moving track (202), described driving stem (301) is arranged in described guide path (103), described inoculation cut module (3) is an one-piece construction with the contact surface (303) of the substratum in described lower cover (2) or is spaced from each other structure in a row.
3. microorganism seperation culture device according to claim 2, is characterized in that: described moving track (202) comprises the smooth section (2021), rising ramp section (2022) and the positioning section (2023) that connect successively.
4. microorganism seperation culture device according to claim 3, is characterized in that: described positioning section (2023) is in depression structure or bulge-structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010612624.0A CN102559476B (en) | 2010-12-30 | 2010-12-30 | A kind of microorganism seperation culture device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010612624.0A CN102559476B (en) | 2010-12-30 | 2010-12-30 | A kind of microorganism seperation culture device |
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| CN102559476A CN102559476A (en) | 2012-07-11 |
| CN102559476B true CN102559476B (en) | 2015-10-14 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104017718B (en) * | 2013-12-31 | 2015-11-18 | 无锡源清天木生物科技有限公司 | Disposable microorganism mutation breeding instrument load plate and year bacterium device |
| CN105695316B (en) * | 2016-04-13 | 2018-04-17 | 中国科学院苏州生物医学工程技术研究所 | Microbial streaking device |
| CN111733204A (en) * | 2020-07-03 | 2020-10-02 | 安徽屾远材料技术有限公司 | Microbial rapid detection membrane and preparation method and use method thereof |
| CN113430241A (en) * | 2021-06-17 | 2021-09-24 | 杨凌质量技术检测检验所 | Food pathogen detection reagent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE602005019385D1 (en) * | 2004-01-22 | 2010-04-01 | Medvet Science Pty Ltd | DEVICE FOR SPREADING MICROBES |
| CN200964413Y (en) * | 2006-09-29 | 2007-10-24 | 姜世民 | Automatization isolated culture plate for organism sample copy |
| CN201116302Y (en) * | 2007-08-23 | 2008-09-17 | 姜世民 | Novel biological sample automatization isolated culture device |
| CN202047055U (en) * | 2010-12-30 | 2011-11-23 | 苏州生物医学工程技术研究所 | Microbe isolated culture device |
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