CN102515889A - Production method for biocontrol water flush fertilizer, fertilizer and application - Google Patents
Production method for biocontrol water flush fertilizer, fertilizer and application Download PDFInfo
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- CN102515889A CN102515889A CN2011104254802A CN201110425480A CN102515889A CN 102515889 A CN102515889 A CN 102515889A CN 2011104254802 A CN2011104254802 A CN 2011104254802A CN 201110425480 A CN201110425480 A CN 201110425480A CN 102515889 A CN102515889 A CN 102515889A
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Abstract
The invention provides a production method for a biocontrol water flush fertilizer, comprising the following steps of: independently fermenting a biocontrol bacillus subtilis HL259 strain, a strain bacillus mucilaginosus krassilnikov strain and a biocontrol streptomyces sp. YL04 strain; adsorbing by an adsorbing medium and then mixing in a ratio of (8 to 10): (6 to 8): (1 to 2) by mass; and mixing with yeast organic materials. The invention further provides an application for the biocontrol water flush fertilizer in prevention and treatment for diseases and insect pests. The water flush fertilizer has obvious prevention and treatment effects on the diseases of crop, thereby greatly reducing seedling disease rate; and the water flush fertilizer can comprehensively provide the nutrients needed by the growth of crop, thereby increasing the yield of crop.
Description
Technical field
The present invention relates to a kind of working method of dashing fertilising, specifically, relate to a kind of working method of dashing fertilising with biocontrol effect.
Background technology
The insect pest of farm crop, disease are the important factor that influences its output all the time, disease and pest make the serious underproduction of farm crop, even total crop failure, therefore, extremely important to effective control of disease and pest.China's agricultural serves as the main prevention and elimination of disease and pests that comes with chemical pesticide, chemical bactericide for a long time, and over nearly 20 years, important effect has been brought into play in using to the safety in production that ensures China's agricultural of chemical pesticide; But cause the Harmony of agricultural tillage to reduce thus with intensive; Be too dependent on chemical fertilizer and agricultural chemicals for a long time, cause the waste of a large amount of Nonrenewable energy resources, the farmland soil property degenerates; Fertility descends, and crop products is residual, quality reduction and food and environmental pollution problems become increasingly conspicuous.Control crop pest through biological control and become the new research focus in agricultural working field in the world at present.Countries in the world are taking practical action to reduce the use of chemical pesticide; The dynamics that increasing drops into biological control; Through the vital movement of its contained mikrobe, increase the supply of plant nutrient or promote plant-growth, improve output; Reduce the sickness rate of disease and pest, thereby improve quality of agricultural product and agroecological environment.
When reducing the chemical pesticide usage quantity, the microbial-bacterial fertilizer of developing biological pesticide or having a biological and ecological methods to prevent plant disease, pests, and erosion characteristic has become a kind of international trend.Relevant expert finds that in research and development a lot of mikrobes and meta-bolites thereof have the dual-use function of biological and ecological methods to prevent plant disease, pests, and erosion and nutrition concurrently both at home and abroad; Maybe the microbiobacterial agent that has nutrition and biological and ecological methods to prevent plant disease, pests, and erosion is respectively had obvious synergistic diseases prevention and short effects such as long after composite, this exploitation that is the microbial-bacterial fertilizer with biological and ecological methods to prevent plant disease, pests, and erosion characteristic provides thinking and reference.The domestic technology that goes up biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is in the starting stage now; Biological and ecological methods to prevent plant disease, pests, and erosion property fertilizer on the market is less; The biological and ecological methods to prevent plant disease, pests, and erosion property fertilizer that dashes the type of executing is more few; And technology is immature, and my company is through developing the bio-organic fertilization fertilizer material of biological and ecological methods to prevent plant disease, pests, and erosion property after the TE, exploitation at present have technological difficulties that the type that the topdresses biological defense fertilization of biocontrol effect need solve mainly contain following some:
1, the selection of organic carrier, the life condition of mikrobe receives organic matter, the C/N ratio; The restriction of a series of conditions such as water cut, the especially quantity of biocontrol strain in the middle of soil and the size of colonization ability largely depend on the characteristic of fertilizer carrier; Domestic technique mainly utilizes the peat composed of rotten mosses or humic acids as organic carrier at present, can satisfy the condition of mikrobe existence, guarantees the number of viable of mikrobe; But the peat composed of rotten mosses and humic acids belong to mineral substance, receive the restriction of national resources, and a large amount of exploitations can destroy ecological; And execute very difficult dissolving in soil, organic matter wherein and humic acids are difficult to influenced fertilizer efficiency by crop utilization; The price of the peat composed of rotten mosses and humic acids also goes up year by year simultaneously, and production cost of products is also improving year by year;
2, the screening of biocontrol bacterial strain; Soil beneficial microorganism resource to having the biological and ecological methods to prevent plant disease, pests, and erosion effect in the multiple soil is investigated; Inquire into its validity; Microorganism to can effectively breeding and have the anti-effect of Johnson & Johnson separates, screens and identifies, makes it have spectrum and strong disease resistance, and solves its separation rejuvenation and culture technique;
3, the customizations seriation problem of fertilizer, present different areas, the constituent content difference that Different Crop is required; But the seriation of fertilizer and customizations are not in place; Need produce different fertilizer according to different prescriptions, in order to avoid cause the waste of nutrition, and geographic nutrition accumulation;
4, effective amount of viable bacteria, generally about 0.2 hundred million/gram, the existence timeliness of bacterial strain is short for the living bacteria count amount of dashing the fertilising series products on the market, and biological effect is not remarkable.
Summary of the invention
Therefore, the present invention's technical problem that will solve provides a kind of working method of dashing fertilising with biological and ecological methods to prevent plant disease, pests, and erosion property effect.Second technical problem that the present invention will solve provides this application of dashing fertilising.
Technical scheme of the present invention is, a kind of biological and ecological methods to prevent plant disease, pests, and erosion property is dashed the working method of fertilising, comprises the preparation of yeast organic raw material and the preparation of microbial inoculum, and it is characterized in that: this method may further comprise the steps:
(1) preparation of yeast organic raw material: will extract the later yeast fermentation broth waste liquid magma of yeast and concentrate, and make concentration, and obtain tawny powder yeast organic raw material through spray dry process then greater than 50%;
The preparation of (2) biological and ecological methods to prevent plant disease, pests, and erosion property complex micro organism fungicide: with subtilis (Bacillus subtilis) HL259; Colloid bacillus cereus (Bacillus mucilaginosus Krassilnikov); After fermenting separately, biological and ecological methods to prevent plant disease, pests, and erosion property streptomycete (Streptomyces sp.) YL04 bacterial classification adsorbs with adsorption medium; Again according to 8-10: the mass ratio of 6-8: 1-2 mixes, and pulverizes;
(3) biological and ecological methods to prevent plant disease, pests, and erosion property is dashed the preparation of fertilising: step (1) yeast organic raw material that obtains and the biological and ecological methods to prevent plant disease, pests, and erosion property complex micro organism fungicide that step (2) obtains are mixed, and the back pulverizing that stirs obtains powdery biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer; The mass ratio that said complex micro organism fungicide accounts for the finished product composite microbiological fertilizer is 10-20%.
Biological and ecological methods to prevent plant disease, pests, and erosion property is dashed fertilising as topdressing with fertile, and fertilizer requires and can supplement the nutrients rapidly, acts on crop rapidly, requires the onset time shorter; Therefore, need to add a large amount of mikrobes, make it act on crop rapidly; And, use long duration of action on a small quantity unlike base manure, treat it in soil, to breed; Surely grow, so the microbial inoculum amount of dashing the fertilising interpolation is bigger.
Above-mentioned biological and ecological methods to prevent plant disease, pests, and erosion property subtilis (Bacillus subtilis) HL259 is provided by China Agricultural University's agronomy and seminar of department of plant pathology of biotechnology institute; Be preserved in Chinese common micro-organisms culture presevation administrative center (abbreviation CGMCC; The Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica), deposit number is CGMCC No.1451, and its concrete proterties is referring to patent 200510104872.3; Above-mentioned biological and ecological methods to prevent plant disease, pests, and erosion property streptomycete (Streptomyces sp.) YL04; Belonging to actinomycetales (Actinomycete) streptomyces (Streptomyces) is also provided by China Agricultural University's agronomy and seminar of department of plant pathology of biotechnology institute; Be preserved in Chinese common micro-organisms culture presevation administrative center; Deposit number is CGMCC No.2884, and its concrete proterties is referring to patented claim 200910080902.X, and open day is on September 9th, 2009.
Preferably, said yeast fermentation broth derives from the zymic production process.
Dash the working method of applying fertilizer according to biological and ecological methods to prevent plant disease, pests, and erosion property of the present invention, be preferably, the said yeast organic raw material of step (1) fineness is greater than 80 orders, and moisture is less than 6%.
Dash the working method of applying fertilizer according to biological and ecological methods to prevent plant disease, pests, and erosion property of the present invention, be preferably, the said total viable count that mixes the back biocontrol fungicide of step (2) reaches 6 * 10
8Individual/more than the gram, moisture≤30%.
Dash the working method of applying fertilizer according to biological and ecological methods to prevent plant disease, pests, and erosion property of the present invention, be preferably, step (3) is added one or more in the NPK, processes seriation, the customizations product of Different Nutrient Elements.NPK adopts product commonly used on the market to get final product.
The present invention also provides by biological and ecological methods to prevent plant disease, pests, and erosion property and has dashed fertilising, living bacteria count>=5 * 10 in the said biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer towards the biological and ecological methods to prevent plant disease, pests, and erosion property that the working method of applying fertilizer obtains
8Individual/more than the gram, N+P
2O
5+ K
2O>=15%, organic>=25%.
The present invention also provides above-mentioned biological and ecological methods to prevent plant disease, pests, and erosion property to dash the application of fertilising on crop is topdressed, and 8-30kg is used on every mu of ground.According to the rich or poor situation of the nutrient of crop fertilizer requirement and soil, slightly adjustment.Impose and to be divided into 1-3 time and to impose.Can impose different number of times with the incidence of disease based on the actual nutrient demand of crop.
Bio-feritlizer in the market is a raw material with humic acid such as feces of livestock and poultry and straw, weathered coals mainly, forms through fermentation maturity, and is water-soluble very poor; Be difficult to be used for produce and dash fertilising, and floor space is big, time-consuming; Atmospheric pollution is serious, is difficult to scale operation, also can cause consequences such as burning seedling if can not become thoroughly decomposed fully; Assorted bacterial content exceeds standard, and life-time service can cause the increase of soil disease.
Bio-feritlizer class classification is mainly base manure and topdresses two kinds, topdress with dash fertilising use the most general, but in the market to dash fertilization effect single; And act on not obviously, and only solve to a certain problem, be mostly to replenish soil and required nutrient of plant and content; Functional single; The present invention remedies existing deficiency of dashing fertilising, on the basis of original patent, improves, and produces the bio-organic fertilization fertilizer of biological and ecological methods to prevent plant disease, pests, and erosion property.
Know-why of the present invention is, the yeast fermentation broth waste liquid that produces when producing yeast is through containing great number of organic matters, amino acid, humic acid, protein and nitrogen, phosphorus, potassium, calcium, magnesium, iron, zinc various nutrient elements in the organic raw material after the spraying drying, and most of nutritive substance is the required nutrient of crop and microorganism growth in these materials; Can replenish crop alimentary and promote the quick breeding of useful microbe in soil in the fertilizer, and for organic raw material also very easily is dissolved in water, small molecules organic matter wherein can be directly by soil and crop utilization; When nutrition be provided for crop, promote plant growth; Can combine fast the formation soil granular with soil particle, the structure of improving the soil makes soil become soft, ventilative, water conservation, guarantor's fertilizer; Effectively improve crop yield; After having added biological and ecological methods to prevent plant disease, pests, and erosion property complex micro organism fungicide outside two, make this dash fertilising when having fertilizer efficiency, also have the effect of drug effect; The interpolation of biocontrol strain can significantly reduce the sickness rate of farmland soil-borne disease; Reduce the input of peasant, reduce pollution, help the development of the ecological agriculture environment to agricultural chemicals and chemical fertilizer.The tandem product of production Different Nutrient Elements; Form high nitrogen, high phosphorus, high potassium and universal tandem product; According to different areas; Different Crop, the different demands of Various Seasonal are used Different products, in order to avoid the enrichment that causes the waste of nutritive element or too much use the nutritive element that unwanted nutritive element causes.
Compared with prior art, the present invention has following advantage:
1, widened and dashed the functional of fertilising: broken the pattern that tradition is dashed the single fertilizer efficiency of fertilising; Biocontrol strain is added in the middle of fertilising, make and dash the function that fertilising has drug effect concurrently, reduce the input of peasant agricultural chemicals; Reduction also is improved quality product to the pollution of environment simultaneously; Through the proportioning of rational biological and ecological methods to prevent plant disease, pests, and erosion property microbial inoculum, guaranteed biological and ecological methods to prevent plant disease, pests, and erosion property effect.
2, safety, environmental protection: use the yeast raw material as the biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer that raw material adds biocontrol strain production have the utilization ratio height, fertilizer loss is few, agricultural-food, soil and underground water is not polluted the characteristics of safety and environmental protection;
3, towards executing biological and ecological methods to prevent plant disease, pests, and erosion property flushing and spreading fertilizer, biocontrol microorganisms directly acts on the infection root of soil-borne disease, directly effect, and the input amount of minimizing agricultural chemicals, the input amount of reduction agricultural chemicals is added biocontrol microorganisms when topdressing, further kill the soil-borne disease bacterium, increasing both production and income;
The series product of 4, biological and ecological methods to prevent plant disease, pests, and erosion property bio-feritlizer add biocontrol microorganisms when supplementing the nutrients, consolidate the restraining effect to soil-borne disease, and biological and ecological methods to prevent plant disease, pests, and erosion property is dashed the content of biocontrol microorganisms in the fertilising up to 5.5 * 10
8Individual/more than the gram, fertilizer efficiency is fast and lasting, can and be absorbed by crop, promotes growth, shows fertilizer efficiency;
5, this product is divided into the proportioning of Different Nutrients content, can be according to Different Crop, and different areas, various seasons, different demands are used different fertilizer, are divided into nitrogenous fertilizer, phosphate fertilizer, potash fertilizer and four kinds of products of versatility, form Products in Seriesization and customizations.
Embodiment
Embodiment 1
The preparation of complex micro organism fungicide: with biological and ecological methods to prevent plant disease, pests, and erosion property subtilis HL259; Colloid bacillus cereus; Three kinds of bacterial classifications of biological and ecological methods to prevent plant disease, pests, and erosion property streptomycete YL04 separately the bacterium liquid after the fermentation with the peat composed of rotten mosses in bacterium liquid: the ratio of the peat composed of rotten mosses=1: 4 is adsorbed; Absorption back solid, powdery microbial inoculum was according to 10: 6: 2 mixed, and the living bacteria count amount of mixing the back composite fungus agent reaches 5.5 * 10
8Individual/more than the gram, moisture remains in 30%, is crushed to 80 orders.
Concentrate extracting the later fermented liquid waste liquid magma of yeast, waste liquid magma concentration is about 7%, makes concentration reach 50% after concentrating, and produces fineness greater than 80 orders through spray dry process then, and moisture is less than 6% tawny powder yeast organic raw material.
The basic proterties of the powdery yeast organic raw material after the spraying drying is seen table 1.
Table 1: the basic proterties of powdery yeast organic raw material
Be to carry out accurate feed proportioning according to the original proportioning of table 2 in 1000 kilograms the stirrer with capacity.
Table 2: the production formula of high nitrogen type biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer
Stirrer stirred 10-15 minute, let the material thorough mixing, was crushed to the 60-80 order after the mixing, and basic proterties is seen table 3, and heavy metal content is not measured far below the industry standard of biological organic fertilizer.
Table 3: the detected result of high nitrogen type biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer
Embodiment 2
The preparation of complex micro organism fungicide and yeast raw material are handled with embodiment 1 in earlier stage.Concrete prescription as follows.
Table 4: the production formula of high phosphorus type biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer
Table 5: the detected result of high phosphorus type biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer
Embodiment 3
The preparation of complex micro organism fungicide and yeast raw material are handled with embodiment 1 in earlier stage.Concrete prescription as follows.
Table 6: the production formula of high potassium type biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer
Table 7: the detected result of high potassium type biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer
Embodiment 4
Biological and ecological methods to prevent plant disease, pests, and erosion property subtilis HL259, colloid bacillus cereus, the mixing quality ratio of three kinds of powdery microbial inoculums of biological and ecological methods to prevent plant disease, pests, and erosion property streptomycete YL04 is 8: 8: 1, other are with embodiment 1.
Embodiment 5
Biological and ecological methods to prevent plant disease, pests, and erosion property subtilis HL259, colloid bacillus cereus, the mixing quality ratio of three kinds of powdery microbial inoculums of biological and ecological methods to prevent plant disease, pests, and erosion property streptomycete YL04 is 8: 6: 2, other are with embodiment 2.
Comparative example 1
Dash fertilising with mineral manure production
1, content standard: total nutrient >=20%
2, working method
1) material proportion: press ammonium sulfate 65%, urea 5%, monoammonium phosphate 15%, vitriolate of tartar 8%, zinc sulfate 5.5%, sal epsom 1%, the ratio batching of borax 0.5%.
2) mix: confected materials is mixed.
3) pulverize: the raw material that mixes is pulverized, and fineness reaches 80 orders.
The basic proterties of finished product sees Table 8
Test Example
The tomato field test effect
Test is handled:
A: base manure: 300 kilograms/mu of fertilizers, 20 kilograms of general calcium of one-level, 15 kilograms of vitriolate of tartar, 10 kilograms of urea
Topdress: (high potassium type) biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer matrix that the yeast organic raw material is produced is topdress (25 kilograms/mu);
B: base manure: 300 kilograms/mu of fertilizers, 20 kilograms of general calcium of one-level, 15 kilograms of vitriolate of tartar, 10 kilograms of urea
Topdress: (high potassium type) biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer that the yeast organic raw material is produced is topdress (25 kilograms/mu).
Matrix is meant sterilizes fertilizer.Topdressing in two processing all is the fertilising of dashing among the embodiment 1.
Sub-district area 20m
2, 3 repetitions are set, district's group is arranged at random, topdresses per 15 days once, and other management are identical with habits fertilization.Data statistical approach: yield data is checked significance with duncan's new multiple range method.
Table 8: the back different treatment of topdressing is to the tomato economical character
Can find out that by table 8 dash fertilising in the back biological and ecological methods to prevent plant disease, pests, and erosion of topdressing and do the nutrient that the processing of topdressing can replenish soil rapidly, plant plant height and stem slightly all are improved; Be significantly higher than the processing of dashing fertilising matrix, explain, apply the biological and ecological methods to prevent plant disease, pests, and erosion flushing and spreading fertilizer and be more conducive to later stage plant height and the thick increase of stem; And fruit tree and single fruit weight also are higher than and dash the fertilising matrix treatments; To apply in the soil after water-soluble towards fertilising, and the living bacteria count amount of dashing in the fertilising is many, all 5.5 * 10
7About individual/gram, can in the middle of soil, grow surely rapidly, directly act on soil and crop, effect very obviously.
Table 9: different treatment is to the influence of tomato production and sickness rate
Can find out that by table 9 seedling rate of two processing is basic identical, but after topdressing processing towards fertilising with towards fertilising matrix; Tangible difference has taken place to have produced in the sickness rate of fruit and the output of crop, explains, the later stage carries out towards spraying fertilizer tomato; Matrix treatments and biological and ecological methods to prevent plant disease, pests, and erosion are bigger towards the effect difference of executing of topdressing, and use biological and ecological methods to prevent plant disease, pests, and erosion property and be more conducive to replenishing rapidly of nutrition towards fertilising, and finally the sickness rate of fruit significantly is lower than towards the processing of fertilising matrix; Act on plant rapidly; Reduce the sickness rate of fruits, be more conducive to the accumulation of fruit, can reduce the sickness rate of fruit.
Can find out that from experiment effect the useful biocontrol microorganisms that biological and ecological methods to prevent plant disease, pests, and erosion property is dashed fertilising can act on plant rapidly, the sickness rate that reduces crop from, help increasing crop yield.
Claims (7)
1. a biological and ecological methods to prevent plant disease, pests, and erosion property is dashed the working method of applying fertilizer, and comprises the preparation of yeast organic raw material and the preparation of microbial inoculum, and it is characterized in that: this method may further comprise the steps:
(1) preparation of yeast organic raw material: will extract the later yeast fermentation broth waste liquid magma of yeast and concentrate, and make concentration, and obtain tawny powder yeast organic raw material through spray dry process then greater than 50%;
The preparation of (2) biological and ecological methods to prevent plant disease, pests, and erosion property complex micro organism fungicide: with subtilis (Bacillus subtilis) HL259; Colloid bacillus cereus (Bacillus mucilaginosus Krassilnikov); After fermenting separately, biological and ecological methods to prevent plant disease, pests, and erosion property streptomycete (Streptomyces sp.) YL04 bacterial classification adsorbs with adsorption medium; Again according to 8-10: the mass ratio of 6-8: 1-2 mixes, and pulverizes;
(3) biological and ecological methods to prevent plant disease, pests, and erosion property is dashed the preparation of fertilising: step (1) yeast organic raw material that obtains and the biological and ecological methods to prevent plant disease, pests, and erosion property complex micro organism fungicide that step (2) obtains are mixed, and the back pulverizing that stirs obtains powdery biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer; The mass ratio that said complex micro organism fungicide accounts for the finished product composite microbiological fertilizer is 10-20%.
2. biological and ecological methods to prevent plant disease, pests, and erosion property according to claim 1 is dashed the working method of fertilising, and it is characterized in that: said yeast fermentation broth derives from the zymic production process.
3. biological and ecological methods to prevent plant disease, pests, and erosion property according to claim 1 is dashed the working method of fertilising, and it is characterized in that: the said yeast organic raw material of step (1) fineness is greater than 80 orders, and moisture is less than 6%.
4. biological and ecological methods to prevent plant disease, pests, and erosion property according to claim 1 is dashed the working method of fertilising, it is characterized in that: the said total viable count that mixes the back biocontrol fungicide of step (2) reaches 6 * 10
8Individual/more than the gram, moisture≤30%.
5. biological and ecological methods to prevent plant disease, pests, and erosion property according to claim 1 is dashed the working method of fertilising, it is characterized in that: step (3) is added one or more in the NPK, processes seriation, the customizations product of Different Nutrient Elements.
6. the described biological and ecological methods to prevent plant disease, pests, and erosion property of claim 1 is dashed fertilising towards the biological and ecological methods to prevent plant disease, pests, and erosion property that the working method of applying fertilizer obtains, and it is characterized in that: living bacteria count>=5 * 10 in the said biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilization fertilizer
8Individual/more than the gram, N+P
2O
5+ K
2O>=15%, organic>=25%.
7. the said biological and ecological methods to prevent plant disease, pests, and erosion property of claim 6 is dashed the application of fertilising on crop is topdressed, and it is characterized in that: 8-30kg is used on every mu of ground.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103319248A (en) * | 2013-06-27 | 2013-09-25 | 苏州禾润农业科技有限公司 | Microorganism fertilizer for preventing and treating valsa mali of lettuce and preparation method thereof |
| CN104447111A (en) * | 2014-11-25 | 2015-03-25 | 上海创博生态工程有限公司 | Microbial preparation for improving tobacco planting soil and preparation method thereof |
| CN106689211A (en) * | 2015-07-30 | 2017-05-24 | 上海绿乐生物科技有限公司 | Trichoderma harzianum-containing biocontrol composite microbial agent and preparation method thereof |
| CN109122181A (en) * | 2018-08-24 | 2019-01-04 | 内蒙古蒙草生态环境（集团）股份有限公司 | A kind of cultural method of landscape lily |
| CN112225595A (en) * | 2020-10-21 | 2021-01-15 | 福建三炬生物科技股份有限公司 | Water-soluble compound microbial fertilizer and preparation method thereof |
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2011
- 2011-12-16 CN CN2011104254802A patent/CN102515889A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103319248A (en) * | 2013-06-27 | 2013-09-25 | 苏州禾润农业科技有限公司 | Microorganism fertilizer for preventing and treating valsa mali of lettuce and preparation method thereof |
| CN103319248B (en) * | 2013-06-27 | 2015-02-11 | 苏州禾润农业科技有限公司 | Microorganism fertilizer for preventing and treating valsa mali of lettuce and preparation method thereof |
| CN104447111A (en) * | 2014-11-25 | 2015-03-25 | 上海创博生态工程有限公司 | Microbial preparation for improving tobacco planting soil and preparation method thereof |
| CN106689211A (en) * | 2015-07-30 | 2017-05-24 | 上海绿乐生物科技有限公司 | Trichoderma harzianum-containing biocontrol composite microbial agent and preparation method thereof |
| CN109122181A (en) * | 2018-08-24 | 2019-01-04 | 内蒙古蒙草生态环境（集团）股份有限公司 | A kind of cultural method of landscape lily |
| CN112225595A (en) * | 2020-10-21 | 2021-01-15 | 福建三炬生物科技股份有限公司 | Water-soluble compound microbial fertilizer and preparation method thereof |
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Application publication date: 20120627 |