CN102415339A - Rapid propagation method of photinia fraseri - Google Patents
Rapid propagation method of photinia fraseri Download PDFInfo
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- CN102415339A CN102415339A CN2011103064586A CN201110306458A CN102415339A CN 102415339 A CN102415339 A CN 102415339A CN 2011103064586 A CN2011103064586 A CN 2011103064586A CN 201110306458 A CN201110306458 A CN 201110306458A CN 102415339 A CN102415339 A CN 102415339A
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Abstract
The invention relates to a rapid propagation method of photinia fraseri, which belongs to the technical field of plant culture and comprises five operation steps of: culture medium preparation, explant collection and pretreatment, isolated culture, rooting seedling domestication and transplanting. The transplanting survival rate of the method reaches higher than 90 percent, few explants are adopted, the mother tree growth development and the tree shape culture are not influenced, hundreds of seedlings can be propagated by adopting one terminal bud or stem section, the propagation number can be greatly improved, the scaled and factory seedling is favorably realized, the propagation speed is accelerated, and the cost is reduced.
Description
Technical field
The invention belongs to trees vegetative propagation field, be specifically related to a kind of fast breeding method of photinia glabra.
Background technology
Photinia glabra is the cottage propagations that adopt on producing more, and cuttage generally adopts branch to make propagating materials, and conventional method is to select sturdy then lignification or semi-lignified branch; Clip is about the slotting fringe of 8-15cm; Otch is smooth smooth, and upper cut is apart from about the terminal bud 1cm, and the blade clip at skill bar top is half the; Rest blade is wiped out, and the slotting fringe base portion that shears is handled back medium the waiting of insertion matrix with root-growing agent and taken root.
The method one is to need a large amount of propagating materialss, the 2nd, and big to the elite stand growth effect of being drawn materials, unfavorable tree-like cultivation; The 3rd, insert fringe at clip and will note keeping the distance of upper cut apart from terminal bud 1cm, time-consuming taking a lot of work, the 4th, reproduction coefficient is lower; General external is 30%, and domesticly have only 20%.
Summary of the invention:
The present invention provides the fast breeding method of a kind of photinia glabra that breeding is few with material, speed is fast, quantity is big.
Technical scheme of the present invention is following:
Step 1: medium preparation: comprise starting medium preparation, proliferated culture medium preparation, culture of rootage preparation; Wherein starting medium is minimal medium with MS; Add 2.0 mg/L 6-BA, 0.15 mg/L NAA, 0.1% active carbon, 3% sucrose and 0.65% agar powder, pH5.8; Proliferated culture medium is a minimal medium with 1/2MS, adds 2.5 mg/L 6-BA, 0.5 mg/L NAA, 3% sucrose and 0.65% agar powder, pH5.8; Root media is a minimal medium with 1/2MS, adds 1.5 mg/L IBA, NAA 0.2 mg/L, 1.5% sucrose and 0.65% agar powder, pH5.8.
Step 2: explant collection and preliminary treatment.In the fine morning in summer, gather the semi-lignified shoot as explant, remove unnecessary cauline leaf; Place beaker with 0.1% liquid detergent solution, soak 15 min, during constantly stir; The beaker mouth is tightened with the gauze covering; Wash 30 min with running water, use distilled water flushing again 3～4 times, flush away surface dirt.Place superclean bench then, with changing 0.1% HgCl over to behind 75% alcohol surface sterilization, 30 s
2Soak 5～7 min in the solution; Constantly shake during this time; After sterile water (distilled water of high pressure steam sterilization) flushing 5～6 times, aseptic filter paper (through the filter paper of high pressure steam sterilization) suck dry moisture cuts terminal bud or with the stem section of 1 axillalry bud with aseptic scalpel (through the scalpel of high steam).
Said superclean bench is after start, after 15 minutes, sprays table top with alcohol with ultra violet lamp again, has wind to go out after the start always, and bacterium can not get in the platform again, belongs to the sterile working process.
Step 3: cultured in vitro: comprise starting cultivation, enrichment culture, culture of rootage, wherein, start and cultivate; With terminal bud or with the stem section of 1 axillalry bud be inoculated into start medium after, place in 23～27 ℃ the culturing room, be light source with the fluorescent lamp; Intensity of illumination is 1500Lx～2 000 Lx; Light application time 10h/d, culturing room's relative moisture 70%～80% is cultivated 20-30 d; Change the indefinite bud that generates over to proliferated culture medium, cultivate 20-25 d, produce more indefinite bud, change the seedling of robust growth over to culture of rootage; The culture of rootage condition is that indoor temperature is 20 ℃, is light source with the fluorescent lamp, and intensity of illumination is 1500 Lx, light application time 12h/d, and culturing room's relative moisture 70%～80%, after culture of rootage 20-25 days, root system grows to 3-5cm, carries out acclimatization and transplants.Said enrichment culture condition is identical with the startup condition of culture.
Step 4: the seedling of taking root domestication and transplanting: when root system grows to the 3-5cm left and right sides; Opening cultivation bottle cap injection 5-10ml distilled water makes medium be immersed in distilled water; Refining seedling 3-5 d transplants to being equipped with in the matrix flowerpot of (peat: perlite: vermiculite=5:3:1 mixes by volume) again.Before transplanting with the dark liquid irrigating root of 20% sterilization ling, to note during transplanting with on the root system with medium rinse out, in case mould contamination influences root growth.Every day, each was sprayed water once sooner or later simultaneously, kept the humidity more than 70%.The refining seedling can move on to outdoor after 1 month, transplanting survival rate reaches more than 90%.
Described peat, perlite, vermiculite are commercially available peat, perlite, vermiculites, mix by volume.
Compare with conventional method, the present invention takes explant quantity few, does not influence elite stand and grows and tree-like cultivation; Take a terminal bud or stem section, just can breed hundreds of strain Cheng Miao, and slotting fringe of tradition breeding can only be bred a plant.
Therefore, use the present invention to carry out the photinia glabra plant breeding, can improve breeding quantity greatly, help to carry out scale, factorial seedling growth, accelerate reproduction speed, reduce cost.In the production of photinia glabra nursery stock, have important practice and dissemination.
Embodiment:
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that embodiment only is used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Year December in April, 2009 to 2010, Academy of Agricultural Sciences's crop science research institute group training research department in the Shanxi Province, the researcher has obtained the group culturation rapid propagating technology of photinia glabra, and has successfully cultivated into tissue cultivating seedling through repetition test.Main through being: as in summer in 2009 in the fine morning, to gather the semi-lignified shoot, remove unnecessary cauline leaf as explant; Place beaker with 0.1% liquid detergent solution, soak 15 min, during constantly stir; The beaker mouth is tightened with the gauze covering; Wash 30 min with running water, use distilled water flushing again 3～4 times, flush away surface dirt.Place superclean bench then, with changing 0.1% HgCl over to behind 75% alcohol surface sterilization, 30 s
2Soak 5～7 min in the solution, during constantly shake, after sterile water (distilled water of high pressure steam sterilization) flushing 5～6 times; Aseptic filter paper (through the filter paper of high steam) suck dry moisture cuts terminal bud or with the stem section of 1 axillalry bud with aseptic scalpel (through the scalpel of high steam), is inoculated into that to start medium (be that minimal medium adds 2.0 mg/L 6-BA, 0.15 mg/L NAA, 0.1% active carbon, 3% sucrose and 0.65% agar powder with MS; PH5.8), placing in 23～27 ℃ the culturing room, is light source with the fluorescent lamp; Intensity of illumination is 1500Lx～2 000 Lx; Light application time 10h/d, culturing room's relative moisture 70%～80% was cultivated 20-30 days; (with 1/2MS is that minimal medium adds 2.5 mg/L 6-BA, 0.5 mg/L NAA to change proliferated culture medium over to; 3% sucrose and 0.65% agar powder, pH5.8) after, condition of culture is with start cultivating.Cultivated 20-25 days, and produced indefinite bud, change over to root media (with 1/2MS is that minimal medium adds 1.5 mg/L IBA, NAA 0.2 mg/L, 1.5% sucrose and 0.65% agar powder, pH5.8).The culture of rootage indoor temperature is 20 ℃, is light source with the fluorescent lamp, and intensity of illumination is 1500Lx; Light application time 12h/d, culturing room's relative moisture 70%～80% is cultivated after 20-25 days; When root system grows to the 3-5cm left and right sides; Open and cultivate bottle cap and inject 5-10ml distilled water, refining seedling 3-5 days is transplanted to matrix (peat: perlite: in the flowerpot of vermiculite=5:3:1) is housed again.Before transplanting transplanting medium is sterilized through high-pressure sterilizing pot; With the dark liquid irrigating root of 20% sterilization ling, to note during transplanting with on the root system with medium rinse out, in case mould contamination influences root growth, simultaneously every day sooner or later each water spray once keep the humidity more than 70%.The refining seedling can move into the land for growing field crops after 1 month, and transplanting survival rate reaches more than 90%.
Remarks:
The hormone composition:
6-BA chinesization formal name used at school: 6-benzyl aminopurine
IBA chinesization formal name used at school: indolebutyric acid
NAA chinesization formal name used at school: methyl
MS minimal medium (Murashige & Skoog 1962):
Claims (4)
1. the fast breeding method of a photinia glabra, its characteristic is made up of the following step:
Step 1, medium preparation: comprise starting medium preparation, proliferated culture medium preparation, culture of rootage preparation; Wherein starting medium is minimal medium with MS; Add 2.0 mg/L 6-BA, 0.15 mg/L NAA, 0.1% active carbon, 3% sucrose and 0.65% agar powder, pH5.8; Proliferated culture medium is a minimal medium with 1/2MS, adds 2.5 mg/L 6-BA, 0.5 mg/L NAA, 3% sucrose and 0.65% agar powder, pH5.8; Root media is a minimal medium with 1/2MS, adds 1.5 mg/L IBA, NAA 0.2 mg/L, 1.5% sucrose and 0.65% agar powder, pH5.8;
Step 2, explant collection and preliminary treatment: in the fine morning in summer, gather the semi-lignified shoot, remove unnecessary cauline leaf as explant; Place beaker with 0.1% liquid detergent solution, soak 15 min, during constantly stir; The beaker mouth is tightened with the gauze covering; Wash 30 min with running water, use distilled water flushing again 3～4 times, flush away surface dirt; Place superclean bench then, with changing 0.1% HgCl over to behind 75% alcohol surface sterilization, 30 s
2Soak 5～7 min in the solution, during constantly shake, behind aseptic water washing 5～6 times, the aseptic filter paper suck dry moisture cuts terminal bud or with the stem section of 1 axillalry bud with aseptic scalpel;
Step 3, cultured in vitro: start to cultivate, with terminal bud or with the stem section of 1 axillalry bud be inoculated into start medium after, place in 23～27 ℃ the culturing room; With the fluorescent lamp is light source, and intensity of illumination is 1500 Lx～2 000 Lx, light application time 10h/d; Culturing room's relative moisture 70%～80%; Cultivated 20-30 days, and changed enrichment culture over to, condition of culture is cultivated with starting; Cultivated 20-25 days, and produced indefinite bud, change culture of rootage over to; The culture of rootage indoor temperature is 20 ℃, is light source with the fluorescent lamp, and intensity of illumination is 1500 Lx, light application time 12h/d, and culturing room's relative moisture 70%～80% was cultivated after 20-25 days, and root system grows to 3-5cm, treats acclimatization and transplants;
Step 4, the seedling of taking root domestication and transplanting: when root system grows to the 3-5cm left and right sides, open the cultivation bottle cap and inject a small amount of distilled water, refining seedling 3-5 days; Transplant again to the container that transplanting medium is housed, before transplanting matrix is carried out disinfection, and spray with sterilization ling; Root system must be rinsed well during transplanting, in case mould contamination influences root growth; Every day, each was sprayed water once sooner or later simultaneously, kept humidity, and the refining seedling can move into the land for growing field crops after 1 month.
2. the fast breeding method of a kind of photinia glabra according to claim 1; The enrichment culture condition in the step 3 of it is characterized in that is in temperature is 23～27 ℃ culturing room, to carry out; With the fluorescent lamp is light source; Intensity of illumination is 1500 Lx～2 000 Lx, light application time 10h/d, culturing room's relative moisture 70%～80%.
3. the fast breeding method of a kind of photinia glabra according to claim 1 is characterized in that transplanting survival rate reaches more than 90%.
4. the fast breeding method of a kind of photinia glabra according to claim 1, it is characterized in that in the step 4 that the transplanting medium preparation is according to peat: the volume ratio of perlite: vermiculite=5:3:1 mixes it.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835314A (en) * | 2012-09-20 | 2012-12-26 | 重庆文理学院 | Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting |
| CN103548685A (en) * | 2013-11-01 | 2014-02-05 | 重庆文理学院 | Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method |
| CN104663434A (en) * | 2015-02-09 | 2015-06-03 | 巴中七彩林业科技有限公司 | Method for tissue culture and rapid propagation of photinia x fraseri pink marble |
| CN105875160A (en) * | 2016-05-04 | 2016-08-24 | 枞阳县熊天然生态农业有限公司 | Photinia fraseri seedling culture method |
| CN111296211A (en) * | 2020-03-12 | 2020-06-19 | 重庆市药物种植研究所 | Rapid propagation method of fritillaria taipaiensis bulbs |
| CN114557278A (en) * | 2022-03-17 | 2022-05-31 | 重庆三峡职业学院 | Tissue culture and rapid propagation method and culture medium for hippeastrum rutilum |
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| CN102124955A (en) * | 2011-01-31 | 2011-07-20 | 江苏农林职业技术学院 | Induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos |
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Patent Citations (1)
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| CN102124955A (en) * | 2011-01-31 | 2011-07-20 | 江苏农林职业技术学院 | Induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835314A (en) * | 2012-09-20 | 2012-12-26 | 重庆文理学院 | Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting |
| CN102835314B (en) * | 2012-09-20 | 2014-10-08 | 重庆文理学院 | Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting |
| CN103548685A (en) * | 2013-11-01 | 2014-02-05 | 重庆文理学院 | Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method |
| CN104663434A (en) * | 2015-02-09 | 2015-06-03 | 巴中七彩林业科技有限公司 | Method for tissue culture and rapid propagation of photinia x fraseri pink marble |
| CN105875160A (en) * | 2016-05-04 | 2016-08-24 | 枞阳县熊天然生态农业有限公司 | Photinia fraseri seedling culture method |
| CN111296211A (en) * | 2020-03-12 | 2020-06-19 | 重庆市药物种植研究所 | Rapid propagation method of fritillaria taipaiensis bulbs |
| CN114557278A (en) * | 2022-03-17 | 2022-05-31 | 重庆三峡职业学院 | Tissue culture and rapid propagation method and culture medium for hippeastrum rutilum |
| CN114557278B (en) * | 2022-03-17 | 2023-01-17 | 重庆三峡职业学院 | Rapid propagation method and culture medium for hippeastrum rutilum tissue culture |
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Application publication date: 20120418 |