CN102206691A - Method for producing xylose oligomer from bagasse - Google Patents
Method for producing xylose oligomer from bagasse Download PDFInfo
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- CN102206691A CN102206691A CN 201110075106 CN201110075106A CN102206691A CN 102206691 A CN102206691 A CN 102206691A CN 201110075106 CN201110075106 CN 201110075106 CN 201110075106 A CN201110075106 A CN 201110075106A CN 102206691 A CN102206691 A CN 102206691A
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Abstract
The invention discloses a method for producing xylose oligomer from bagasse. The necessary condition for the method for preparing xylose oligomer is enzymolysis reaction which can decompose xylan into xylose oligomer. The preparation method before enzymolysis reaction can have different results when different raw materials are adopted; and the results embody a concentrated reflection on extraction rate, quality and cost which determine the competitive power in the market, market share and benefit of the xylose oligomer. By using steam explosion and acidic microwave hydrolysis, the invention increases the extraction rate of xylan to 28%; anion-exchange columns are utilized to regulate the pH value of the material, so that the acid value of the coarse syrup can achieve the technical index range without adding acid or alkali, thereby achieving the goals of stabilizing the product quality and increasing the production/extraction yield. The invention is scientific and reasonable and has the advantage of high extraction rate; and the obtained xylose oligomer has the advantages of good quality and low production cost.
Description
Technical field
What the present invention relates to is a kind of method of producing xylo-oligosaccharide, especially produces the method for xylo-oligosaccharide with bagasse, belongs to biological technical field.
Background technology
Xylo-oligosaccharide is one of kind that propagation bifidus bacillus function is the strongest in the polymerization carbohydrate, its effect is nearly 20 times of other polymerization carbohydrates, the enzyme that does not have the hydrolysis xylo-oligosaccharide in the human gastrointestinal tract, so it can directly enter and be preferably bifidus bacillus in the large intestine and utilize, promote bifidus bacillus propagation to produce multiple organic acid simultaneously.Reduce the enteron aisle pH value, suppress harmful bacteria growing, probiotic bacterium is bred in a large number at enteron aisle, reach certain health-care effect.In addition, xylo-oligosaccharide also has many particular performances, decompose such as being difficult to the human consumption enzyme, its Energy value is almost nil, neither influences blood sugar concentration, does not also increase insulin level in the blood sugar, and can not form fatty deposits, so can in low-energy food, play a role, satisfied those to greatest extent and liked sweets and worry diabetes and the people's of obesity requirement, so diabetics, adiposis patient and hypoglycemosis can relievedly eat per capita; To acid, Heat stability is good, difficult fermentation, effectively intake is few.Effect research to xylo-oligosaccharide is very ripe, at present the research of xylo-oligosaccharide is mainly concentrated on the preparation aspect of xylo-oligosaccharide, how improves aspect the extraction yield and raw material of xylo-oligosaccharide.Xylo-oligosaccharide preparation method's prerequisite is an enzyme digestion reaction, and making the xylan enzymolysis by enzyme digestion reaction is xylo-oligosaccharide.And the preparation method before enzyme digestion reaction, adopt different raw materials to have bigger difference, and diverse ways can produce different results, be reflected in extraction yield, quality and cost aspect in these result sets, and this several aspects exactly, the xylo-oligosaccharide competitive capacity in market, share of market and benefit have been determined.
The raw material of preparation xylo-oligosaccharide mainly is a hemicellulose, and the raw material that is fit to the preparation xylo-oligosaccharide at present is relatively good with corn cob and Caulis Sacchari sinensis leaf.And relevant report is not seen in the research for preparing xylo-oligosaccharide with bagasse.In fact the molecular chain structure of bagasse fibre is relatively to be fit to the preparation xylo-oligosaccharide.The main component of bagasse is: Mierocrystalline cellulose 45～50%, hemicellulose 22～36%, xylogen 18～23%, cerosin 2～4%, soluble solid 1～3%.The hemicellulose main component is the wood sugar that contracts (claiming pentosan again) more, molecular formula (C5H804) n, and mean polymerisation degree is generally about 150, and is more much lower than cellulosic degree of polymerization, is easier to hydrolysis and changes xylo-oligosaccharide, wood sugar and pectinose into.Bagasse is as a kind of byproduct in sugaring industry or the beverage/food industry, all be used for raw material at ordinary times as papermaking, just use when bagasse is used for papermaking wherein be the Mierocrystalline cellulose composition, hemicellulose components can only be as waste discharge, and environment is polluted.If xylo-oligosaccharide composition contained in the bagasse is extracted, the added value that is produced is higher, economic benefit can be bigger, therefore, xylo-oligosaccharide is extracted in further investigation from bagasse, and it is higher especially to extract quality, cost is lower, quality is better, and the xylo-oligosaccharide that yield is higher seems more meaningful.
Goal of the invention
Purpose of the present invention just provides a kind of method of producing xylo-oligosaccharide with bagasse, and it is low to overcome produced in conventional processes xylo-oligosaccharide yield, poor quality, the deficiency that cost is high.
The realization of the object of the invention, based on such principle: contain wood sugar (hemicellulose) in the bagasse, and wood sugar contains 30% xylan approximately, xylan is the raw material of xylo-oligosaccharide and Xylitol, but fibre composition wherein---Mierocrystalline cellulose and xylogen are difficult to decompose, for making the xylan in the bagasse be easy to dissolve, adopt steam explosion to handle, moment discharged in normal atmosphere after bagasse after promptly pulverizing heated with high-pressure water vapor, make its cell walls swelling of fiber, because steam explosion is handled the fibrous texture that can destroy bagasse, makes xylan be easier to dissolve extraction.According to above-mentioned principle, the realization of the object of the invention is because taked following technique measures: a kind of method of producing xylo-oligosaccharide with bagasse, comprise three steps, and be respectively pre-treatment; Enzyme decomposes; Concentrate; Described pre-treatment step is:
(1) get bagasse and pulverize and to be 1-5 millimeter coarse grain, place in the sealed vessel, by being steam heated to 190-260 ℃, kept 2-5 minute, at 0.1-1 abrupt release pressure in time second, make bagasse cell walls swelling of fiber then, it is standby to obtain expanded bagasse;
(2) get expanded bagasse and add 15-25 times of water gaging, adjust with acid again that to make the solution pH value be 4.0-5.0, kept 1-2 hour to 80-90 ℃, filter with the 200-400 mesh filter screen earlier with microwave heating, filtrate centrifugation more then, it is standby to obtain supernatant liquor;
(3) with supernatant liquor according to conventional speeds slowly by commercially available cation exchange resin column, filtrate more routinely speed collect filtrate slowly by commercially available active carbon column, vacuum concentration is doubly measured to such an extent that concentrated solution is standby to the 4-6 of raw material under 60-70 ℃ of condition;
(4) get concentrated solution and slowly pass through commercially available anion-exchange resin column according to conventional speeds, make filtrate keep PH3.0-5.0, it is standby to obtain xylan solution;
Described enzyme decomposition step refers to the process with conventional zytase enzymolysis xylan solution, obtains xylo-oligosaccharide solution through behind the enzymolysis;
Described enrichment step is:
The xylo-oligosaccharide solution vacuum concentration under 60-70 ℃ of condition of zytase enzymolysis of learning from else's experience places the crystallization under 60-70 ℃ of environment of crystallization in motion case again to being saturated solution, and control per hour reduces temperature 0.5-2 ℃, until the xylo-oligosaccharide that obtains higher degree.
Take the present invention of above-mentioned measure, with steam explosion and acid microwave hydrolysis, can improve the xylan extraction yield, its extraction yield is up to 28%, and traditional technology is to extract with adding the NaOH heating hydrolysis, and its extraction yield is low, and extraction yield only is about 17%.In xylo-oligosaccharide was produced, whether the syrup acid number was controlled in the proper range, for the quality of massecuite crystallization effect great relation was arranged.Traditional method mainly in subsequent handling to the adjustment of compensating property of raw sugar slurry pH value, promptly, it is added acid or adds alkali according to the value height of output raw sugar slurry PH, make its pH value reach the technic index scope.The result who does like this is: the intermediate that has purified adds a spot of mineral ion again, and operation easier is big, causes quality product easily to fluctuate, and produces the sugar producing rate fluctuation greatly.And the present invention utilizes anion-exchange column that the material pH value is adjusted, and under the prerequisite that does not add soda acid, makes raw sugar slurry acid number reach the technic index scope, has reached stabilized product quality and has improved the purpose of producing sugar producing rate.The present invention is scientific and reasonable, the extraction yield height, and gained xylo-oligosaccharide quality better makes the production cost of xylo-oligosaccharide reduce because extraction yield is high.
Embodiment
Embodiment 1
(1) getting that 100 kilograms of bagasse pulverize is 3 millimeters coarse grain, places in the sealed vessel, keeps 3 minutes by being steam heated to 200 ℃, at 0.1-1 abrupt release pressure in time second, makes bagasse cell walls swelling of fiber then, and it is standby to obtain expanded bagasse;
(2) get expanded bagasse and add 2000 kg of water, adding citric acid again, to make the solution pH value be 4.5, ℃ kept 1.5 hours with microwave heating to 85, filters to get filtrate with 200 mesh filter screens earlier, then filtrate again the supercentrifuge by 5000 rev/mins carry out centrifugation, it is standby to obtain supernatant liquor;
(3) with supernatant liquor according to conventional speeds slowly by commercially available cationic exchange coloum, filtrate slowly by commercially available active carbon column, is collected filtrate again, the concentrated solution of vacuum concentration to 500 kilogram is standby under 60-70 ℃ of condition;
(4) get concentrated solution and slowly pass through commercially available anion-exchange column according to conventional speeds, keep filtrate PH4.5, it is standby to obtain xylan solution;
(5) adding activity in xylan solution is the commercially available zytase of 20,000 activity unit/milligrams, is enzymolysis 100 minutes under 55 ℃ the condition in temperature, obtains the xylo-oligosaccharide solution behind the enzymolysis:
(6) learnt from else's experience xylo-oligosaccharide solution vacuum concentration under 60 ℃ of conditions of zytase enzymolysis places the crystallization under 60 ℃ of environment of crystallization in motion case again to being the saturated solution state, and control per hour reduces by 1 ℃ of temperature, until the xylo-oligosaccharide that obtains higher degree.
Embodiment 2
(1) getting that 100 kilograms of bagasse pulverize is 5 millimeters coarse grain, places in the sealed vessel, keeps 2 minutes by being steam heated to 260 ℃, at 0.1-1 abrupt release pressure in time second, makes bagasse cell walls swelling of fiber then, and it is standby to obtain expanded bagasse;
(2) get expanded bagasse and add 2000 kg of water, adding hydrochloric acid again, to make the solution pH value be 5.0, ℃ kept 1.5 hours with microwave heating to 90, filters to get filtrate with 400 mesh filter screens earlier, then filtrate again the supercentrifuge by 5000 rev/mins carry out centrifugation, it is standby to obtain supernatant liquor;
(3) identical with embodiment 1 step (3);
(4) identical with embodiment 1 step (4);
(5) adding activity in xylan solution is the commercially available liquid zytase of 10,000 activity unit/milliliters, is enzymolysis 100 minutes under 55 ℃ the condition in temperature, obtains the xylo-oligosaccharide solution behind the enzymolysis;
(6) learnt from else's experience xylo-oligosaccharide solution vacuum concentration under 70 ℃ of conditions of zytase enzymolysis places the crystallization under 70 ℃ of environment of crystallization in motion case again to being the saturated solution state, and control per hour reduces by 1.5 ℃ of temperature, until the xylo-oligosaccharide that obtains higher degree.
Embodiment 3
(1) step (1) with embodiment 1 is identical;
(2) get expanded bagasse and add 2000 kg of water, adding citric acid again, to make the solution pH value be 4.0, ℃ kept 1.5 hours with microwave heating to 90, filters to get filtrate with 400 mesh filter screens earlier, then filtrate again the supercentrifuge by 5000 rev/mins carry out centrifugation, it is standby to obtain supernatant liquor;
All the other steps are identical with embodiment 1.
Claims (1)
1. a method of producing xylo-oligosaccharide with bagasse comprises three steps, is respectively pre-treatment; Enzyme decomposes; Concentrate; It is characterized in that described pre-treatment step is:
(1) get bagasse and pulverize and to be 1-5 millimeter coarse grain, place in the sealed vessel, by being steam heated to 190-260 ℃, kept 2-5 minute, at 0.1-1 abrupt release pressure in time second, make bagasse cell walls swelling of fiber then, it is standby to obtain expanded bagasse;
(2) get expanded bagasse and add 15-25 times of water gaging, adjust with acid again that to make the solution pH value be 4.0-5.0, kept 1-2 hour to 80-90 ℃, filter with the 200-400 mesh filter screen earlier with microwave heating, filtrate centrifugation more then, it is standby to obtain supernatant liquor;
(3) with supernatant liquor according to conventional speeds slowly by commercially available cation exchange resin column, filtrate more routinely speed collect filtrate slowly by commercially available active carbon column, vacuum concentration is doubly measured to such an extent that concentrated solution is standby to the 4-6 of raw material under 60-70 ℃ of condition;
(4) get concentrated solution and slowly pass through commercially available anion-exchange resin column according to conventional speeds, make filtrate keep PH3.0-5.0, it is standby to obtain xylan solution;
Described enzyme decomposition step refers to the process with conventional zytase enzymolysis xylan solution, obtains xylo-oligosaccharide solution through behind the enzymolysis;
Described enrichment step is:
The xylo-oligosaccharide solution vacuum concentration under 60-70 ℃ of condition of zytase enzymolysis of learning from else's experience places the crystallization under 60-70 ℃ of environment of crystallization in motion case again to being saturated solution, and control per hour reduces temperature 0.5-2 ℃, until the xylo-oligosaccharide that obtains higher degree.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205472A (en) * | 2013-04-10 | 2013-07-17 | 江苏科技大学 | Method for preparing xylooligosaccharide by using mulberry branches |
| CN103614435A (en) * | 2013-12-02 | 2014-03-05 | 广西壮族自治区农业科学院农产品加工研究所 | Method for preparing xylo-oligosaccharide from bagasse |
| CN104087634A (en) * | 2014-07-11 | 2014-10-08 | 清华大学 | Method for preparing xylooligosaccharide by using sorgo fermentation residue |
| CN104231098A (en) * | 2014-08-27 | 2014-12-24 | 华南理工大学 | Method for preparing xylooligosaccharide from bagasse by ethanol/water pretreatment |
| CN106555015A (en) * | 2017-01-22 | 2017-04-05 | 广西农垦糖业集团良圻制糖有限公司 | A kind of bagasse prepares the preprocess method of xylose |
| CN106744938A (en) * | 2016-12-17 | 2017-05-31 | 段清 | A kind of method that utilization bagasse produces decoloration active carbon high |
| CN107529517A (en) * | 2016-07-09 | 2018-01-02 | 王玲燕 | A kind of method that nitrogen carbon molecular sieve is prepared by bagasse |
| CN108729285A (en) * | 2018-05-08 | 2018-11-02 | 昆明理工大学 | A kind of method of the puffing extraction bagasse hemicellulose of microwave- |
| CN109170461A (en) * | 2018-09-03 | 2019-01-11 | 溧阳市迪贝乐生物科技有限公司 | A kind of oligofructose solid beverage and preparation method thereof |
| CN109354628A (en) * | 2018-07-03 | 2019-02-19 | 中科宜康（北京）生物科技有限公司 | A kind of preparation method and applications of xylo-oligosaccharide |
| CN111944332A (en) * | 2020-08-26 | 2020-11-17 | 湖州思源颜料有限公司 | Method capable of remarkably improving coloring power of iron oxide red |
| CN112203672A (en) * | 2018-05-30 | 2021-01-08 | 三井制糖株式会社 | Obesity inhibitor, antidementia agent, deodorant, antiaging agent, anti-glycation agent, anti-type I allergy agent, antihypertensive agent, flavor improving agent, muscle strengthening agent and bone metabolism improving agent |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205472A (en) * | 2013-04-10 | 2013-07-17 | 江苏科技大学 | Method for preparing xylooligosaccharide by using mulberry branches |
| CN103205472B (en) * | 2013-04-10 | 2015-04-15 | 江苏科技大学 | Method for preparing xylooligosaccharide by using mulberry branches |
| CN103614435A (en) * | 2013-12-02 | 2014-03-05 | 广西壮族自治区农业科学院农产品加工研究所 | Method for preparing xylo-oligosaccharide from bagasse |
| CN103614435B (en) * | 2013-12-02 | 2016-05-04 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of method of preparing xylo-oligosaccharide from bagasse |
| CN104087634A (en) * | 2014-07-11 | 2014-10-08 | 清华大学 | Method for preparing xylooligosaccharide by using sorgo fermentation residue |
| CN104231098A (en) * | 2014-08-27 | 2014-12-24 | 华南理工大学 | Method for preparing xylooligosaccharide from bagasse by ethanol/water pretreatment |
| CN107529517A (en) * | 2016-07-09 | 2018-01-02 | 王玲燕 | A kind of method that nitrogen carbon molecular sieve is prepared by bagasse |
| CN107529515A (en) * | 2016-07-09 | 2018-01-02 | 王玲燕 | A kind of method that nitrogen carbon molecular sieve is prepared by bagasse |
| CN107529516A (en) * | 2016-07-09 | 2018-01-02 | 王玲燕 | A kind of method that nitrogen carbon molecular sieve is prepared by bagasse |
| CN107572520A (en) * | 2016-07-09 | 2018-01-12 | 王玲燕 | A kind of method that nitrogen carbon molecular sieve is prepared by bagasse |
| CN107572521A (en) * | 2016-07-09 | 2018-01-12 | 王玲燕 | A kind of method that nitrogen carbon molecular sieve is prepared by bagasse |
| CN106744938A (en) * | 2016-12-17 | 2017-05-31 | 段清 | A kind of method that utilization bagasse produces decoloration active carbon high |
| CN106555015A (en) * | 2017-01-22 | 2017-04-05 | 广西农垦糖业集团良圻制糖有限公司 | A kind of bagasse prepares the preprocess method of xylose |
| CN106555015B (en) * | 2017-01-22 | 2020-05-19 | 广西糖业集团良圻制糖有限公司 | Pretreatment method for preparing xylose from bagasse |
| CN108729285A (en) * | 2018-05-08 | 2018-11-02 | 昆明理工大学 | A kind of method of the puffing extraction bagasse hemicellulose of microwave- |
| CN112203672A (en) * | 2018-05-30 | 2021-01-08 | 三井制糖株式会社 | Obesity inhibitor, antidementia agent, deodorant, antiaging agent, anti-glycation agent, anti-type I allergy agent, antihypertensive agent, flavor improving agent, muscle strengthening agent and bone metabolism improving agent |
| CN109354628A (en) * | 2018-07-03 | 2019-02-19 | 中科宜康（北京）生物科技有限公司 | A kind of preparation method and applications of xylo-oligosaccharide |
| CN109170461A (en) * | 2018-09-03 | 2019-01-11 | 溧阳市迪贝乐生物科技有限公司 | A kind of oligofructose solid beverage and preparation method thereof |
| CN111944332A (en) * | 2020-08-26 | 2020-11-17 | 湖州思源颜料有限公司 | Method capable of remarkably improving coloring power of iron oxide red |
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