CN102154108A - Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof - Google Patents
Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof Download PDFInfo
- Publication number
- CN102154108A CN102154108A CN2011100212124A CN201110021212A CN102154108A CN 102154108 A CN102154108 A CN 102154108A CN 2011100212124 A CN2011100212124 A CN 2011100212124A CN 201110021212 A CN201110021212 A CN 201110021212A CN 102154108 A CN102154108 A CN 102154108A
- Authority
- CN
- China
- Prior art keywords
- liquid
- bacterium
- xylose residue
- furfural dregs
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a pretreatment fungicide for xylose residue or furfural residue, a preparation method and an application thereof, belonging to the technical field of agricultural microbiology. The invention relates to a microbial composite fungicide specially used for promoting the xylose residue or the furfural residue to degrade and improving the production efficiency of methane. The invention firstly adopts liquid fermentation technology to purely breed and ferment all components of the microbial fungicide, and then the components are compounded. The preparation method of the pretreatment fungicide comprises: 0.20-0.40 parts by weight of trichoderma viride liquid, 0.10-0.15 parts by weight of phanerochaete chrysosporium liquid, 0.05-0.10 parts by weight of aspergillus niger liquid, 0.08-0.16 parts by weight of aspergillus oryzae liquid and 0.05-0.10 parts by weight of trametes versicolor liquid, and the dry weight of fermentation hyphae is more than 20g/L. The composite fungicide can be used for improving the composite fungicide speed of the xylose residue or the furfural residue as well as the methane production efficiency; the methane production efficiency is improved by 30-60%; the fermentation time is shortened; the start period is shortened by 3-10 days; and the invention is also good in the effects on the aspects of pretreatment of smashed straws, and methane production.
Description
Technical field
The invention belongs to agriculture microbial technology field, particularly a kind of xylose residue or furfural dregs pre-treatment microbial inoculum and preparation method thereof and uses thereof.
Background technology
Along with China's rapid economic development, energy demand increases year by year, and the energy shortage problem also becomes increasingly conspicuous.At present China greatly develops the shortage with the reply fossil energy of the renewable energy source that comprises biogas, realizes the Sustainable development of China's economy.Aspect the biogas raw material, except feces of livestock and poultry, behaving most, what attract attention is to utilize crop straws for producing biogas.Yet stalk itself is difficult to abundant degraded, has had a strong impact on its gas producing efficiency and use range, still is in the research and development stage so far.Xylose residue is the byproduct that produces in the Xylitol production process.In recent years, along with China's food and the chemical industry demand to Xylitol increases year by year, producer and output that China produces Xylitol are also rising.Usually produce the xylose residue 26-28 ton that 1 ton of Xylitol can produce water content 70%-80%.These waste residues take a large amount of places and deposit, and in spring and summer season the plant-growth of surrounding enviroment are caused and to have a strong impact on, and environment is caused severe contamination.The raw material of producing Xylitol at present is corn cob, cotton seed hulls and bagasse etc., so fibrous material content is abundant in the xylose residue, its content is usually at 50%-60%, and pulverize powder after peracid treatment, being convenient to microbial process, being difficult for crust, is a kind of good raw material of producing biogas.China is one of the major country of production of furfural and export State, can produce a large amount of furfuraldehyde waste slags in its production process.Furfural product per ton is accompanied by residue discharging more than 10 tons.Nearly 30,000,000 tons of China's annual discharging furfuraldehyde waste slag.Because Mierocrystalline cellulose, hemicellulose and content of lignin are higher in xylose residue or the furfural dregs, be difficult to degraded, so its gas producing efficiency is under some influence.At xylose residue and furfural dregs self-characteristic, develop corresponding microbiological treatment microbial inoculum, can improve the efficient that xylose residue or furfural dregs are produced biogas, and help guaranteeing that cold condition guarantees that biogas engineering normally moves down in the winter time.
Chinese patent file 200510077792.3 discloses a kind of fermentation process of composite fungus agent pretreated straw, and its microbial inoculum consists of Trichoderma, subtilis and Bacillus licheniformis.Stalk through pulverize, wetting back adding composite fungus agent and bicarbonate of ammonia carries out biogas fermentation after carrying out stack retting.Be improved through pretreated stalk fermentation speed and aerogenesis speed, and the aerogenesis time shortens.Its microbial inoculum of developing can be used for agricultural wastes pre-treatment such as maize straw, straw, straw, and use range is extensive.Chinese patent file 200810140400.7 discloses a kind of used for methane tank complex micro organism fungicide and preparation method thereof.This invention has proposed a kind of used for methane tank complex micro organism fungicide, is made up of Pseudomonas fluorescens, waxy Bacillus, subtilis and four kinds of microorganisms of Bacillus circulans.This microbial inoculum not only can promote methane-generating pit normal gas producing in winter, and can utilize stalk to produce biogas as main raw material.Wan Chujun etc. the influence [J] that microbial pretreatment rape stalk heap improves biogas output. Transactions of the Chinese Society of Agricultural Engineering, 2010,26:267-271.) utilize subtilis, Bacillus licheniformis, variable color bolt bacterium, Phanerochaete chrysosporium to prepare the degradation mix bacterium agent of rape stalk.Above-mentioned three files have all only been developed corresponding microbiobacterial agent and have been used for the biogas fermentation preprocessing process at stalk, other industrial fiber raw materials (as xylose residue, furfural dregs) are not then had relate to.Than stalk, xylose residue or furfural dregs acidity are higher, and contain a small amount of furfural, and multiple microorganism (especially yeast and bacterium) activity is all had strong restraining effect.Therefore, it is very important for improving its fermenting speed and gas producing efficiency to develop corresponding pre-treatment microbial inoculum targetedly.
Summary of the invention
The object of the invention provides a kind of xylose residue or pretreated microbial inoculum of furfural dregs of being used for, and this microbial inoculum can significantly improve xylose residue or furfural dregs fermenting speed at the own characteristic of xylose residue or furfural dregs, guarantees xylose residue or furfural dregs production biogas under the cold condition.In addition, this microbial inoculum is also having good effect aspect the stalk pre-treatment.
Technical solution of the present invention is as follows: a kind of xylose residue or furfural dregs pre-treatment microbial inoculum, be to be mixed and made into by the bacterium liquid that viride, Phanerochaete chrysosporium, aspergillus niger, aspergillus oryzae, five kinds of bacterium of variable color bolt bacterium obtain after liquid state fermentation, it is as follows by weight: trichoderma viride liquid 0.20-0.40, Phanerochaete chrysosporium bacterium liquid 0.10-0.15, black-koji mould liquid 0.05-0.10, aspergillus oryzae liquid 0.08-0.16, variable color bolt bacterium bacterium liquid 0.05-0.10; Above-mentioned fermented hypha weight (dry weight) is more than the 20g/L.
Xylose residue or furfural dregs pre-treatment microbial inoculum and preparation method thereof, it comprises the steps: 1. these five kinds of bacterium of viride, Phanerochaete chrysosporium, aspergillus niger, aspergillus oryzae and variable color bolt bacterium to be produced five steps and obtain trichoderma viride liquid, Phanerochaete chrysosporium bacterium liquid, black-koji mould liquid, aspergillus oryzae liquid, variable color bolt bacterium bacterium liquid respectively according to slant strains activation, shake flask fermentation, seeding tank liquid state fermentation, the liquid state fermentation of production fermentor tank respectively;
2. bacterium liquid is composite, and above-mentioned five kinds of bacterium bacterium liquid are mixed and made into liquid dosage form according to the above ratio.
Xylose residue or furfural dregs pre-treatment microbial inoculum and preparation method thereof add solid packing in the bacterium liquid after it is characterized in that trichoderma viride liquid, Phanerochaete chrysosporium bacterium liquid, black-koji mould liquid, aspergillus oryzae liquid, variable color bolt bacterium bacterium liquid is mixed in proportion and make solid dosage.
Described solid packing is one or more mixing in xylose residue, furfural dregs, the peat composed of rotten mosses, the diatomite, and its total consumption is 4-10 times of bacterium liquid gross weight after mixing.
The purposes of xylose residue or furfural dregs pre-treatment microbial inoculum is characterized in that described xylose residue or furfural dregs pre-treatment microbial inoculum are used for xylose residue or the pre-treatment of furfural dregs before carrying out biogas fermentation.
Realize the present invention for convenience, the present invention puts down in writing following substratum and cultural method, but bacterium culture medium and cultural method are not limited thereto, and adopt other ordinary method that used bacterial classification is fermented and also can obtain trichoderma viride liquid, Phanerochaete chrysosporium bacterium liquid, black-koji mould liquid, aspergillus oryzae liquid, the variable color bolt bacterium bacterium liquid that the present invention mentions.
The strain activation and culture base of described viride, Phanerochaete chrysosporium, aspergillus niger, aspergillus oryzae and variable color bolt bacterium comprises: potato extracting solution 200 grams, and glucose 20 grams, 1000 milliliters in tap water, agar 20 grams, pH 5.0.Wherein the potato extracting solution is meant, potato 200 grams, and stripping and slicing is boiled after 30 minutes and is filtered back gained clarification extracting solution with crocus cloth (80-150 order).
1) the liquid state fermentation culture condition of described viride is: yeast extract paste 15 grams, glucose 20 grams, Microcrystalline Cellulose 24g, corn steep liquor 10 grams, wheat bran 20 grams, potassium primary phosphate 1.8 grams, sal epsom 0.9 gram, ammonium sulfate 6 grams, ferrous sulfate 0.05 gram, cobalt chloride 0.4 gram, calcium chloride 0.5 gram, 0.1 milliliter of defoamer, 1000 milliliters in tap water, pH5.0.
Viride spore suspension (10
7Individual/milliliter) inoculum size 5%-10%(v/v), culture temperature 28-32 ℃, 200 rev/mins of rotating speeds, air flow 1:1(ventilation volume ratio is a benchmark with the fermentor tank liquid amount) cultivate 1-3 days to logarithmic phase acquisition liquid seeds.
2) the liquid state fermentation culture condition of Phanerochaete chrysosporium bacterium liquid: glucose 15 grams, potassium primary phosphate 3.6 grams, ammonium sulfate 2.4 grams, corn steep liquor 10 grams, wheat bran 5 grams, sal epsom 0.3 gram, calcium chloride 0.6 gram, 5.2 milligrams in ferrous sulfate, 1.8 milligrams of manganous sulfates, 2.2 milligrams in zinc sulfate, 0.8 milligram in copper sulfate, 1000 milliliters in tap water, defoamer 0.1ml, pH6.0.
Phanerochaete chrysosporium spore suspension (10
6Individual/milliliter) inoculum size 5%-10%(v/v), culture temperature 25-28 ℃, 150 rev/mins of rotating speeds, air flow 1:1(ventilation volume ratio is a benchmark with the liquid amount) and incubation time 2-5 days obtains liquid seeds to logarithmic phase.
3) variable color bolt bacterium bacterium liquid liquid state fermentation culture condition: glucose 15 grams, potassium primary phosphate 3.8 grams, ammonium sulfate 2.2 grams, corn steep liquor 12 grams, wheat bran 10 grams, sal epsom 0.3 gram, calcium chloride 0.6 gram, 4.6 milligrams in ferrous sulfate, 2.2 milligrams of manganous sulfates, 2.6 milligrams in zinc sulfate, 1.3 milligrams in copper sulfate, 1000 milliliters in tap water, defoamer 0.1ml, pH6.0.
Spore suspension (10
6Individual/milliliter) inoculum size 5%-10%(v/v), culture temperature 25-28 ℃, 150 rev/mins of rotating speeds, air flow 1:1(ventilation volume ratio is a benchmark with the liquid amount) and incubation time 2-5 days obtains liquid seeds to logarithmic phase.
4) the liquid state fermentation culture condition of aspergillus niger:, glucose 20 grams, dregs of beans 30 grams, wheat bran 20 grams, potassium primary phosphate 1.8 grams, sal epsom 0.9 gram, ammonium nitrate 1.5 grams, 0.1 milliliter of defoamer, 1000 milliliters in tap water, pH 5.0.
Black-koji mould spore suspension (10
7Individual/milliliter) inoculum size 5%-10%(v/v), culture temperature 28-32 ℃, 200 rev/mins of rotating speeds, air flow 1:1(ventilation volume ratio is a benchmark with the fermentor tank liquid amount) cultivate 1-3 days to logarithmic phase acquisition liquid seeds.
5) the liquid state fermentation culture condition of aspergillus oryzae:, glucose 10 grams, starch 40, dregs of beans 30 grams, wheat bran 20 grams, potassium primary phosphate 1.5 grams, sal epsom 0.2 gram, ammonium sulfate 0.4 gram, calcium chloride 0.6g, Repone K 0.4g, 0.1 milliliter of defoamer, 1000 milliliters in tap water, pH 5.0.Aspergillus oryzae spore suspension (10
7Individual/milliliter) inoculum size 5%-10%(v/v), culture temperature 28-32 ℃, 200 rev/mins of rotating speeds, air flow 1:1(ventilation volume ratio is a benchmark with the fermentor tank liquid amount) cultivate 1-3 days to logarithmic phase acquisition liquid seeds.
The spore suspension that reaches described in the above-mentioned explanation is meant, utilize the aaerosol solution that contain certain spore quantity of sterilized water (tap water was sterilized 30 minutes down at 121 ℃, and containing volume fraction is 0.1% tween 80) to trichoderma viride, black-koji mould, aspergillus oryzae, variable color bolt bacterium and Phanerochaete chrysosporium inclined-plane flushing back dilution certain multiple gained.
What clearly do not limit in the index method of mentioned microorganism microbial inoculum all can carry out according to prior art.Complex micro organism fungicide use temperature of the present invention is more than 15 ℃, and fermentation raw material is one of xylose residue, furfural dregs the two or the two and the mixture of bicarbonate of ammonia, ammoniacal liquor the two or one of them.
It is composite that the present invention adopts five kinds of fungies to carry out, Phanerochaete chrysosporium wherein and variable color bolt bacterium can degrade the furfural component in xylose residue or the furfural dregs and Mierocrystalline cellulose wherein, xylogen and hemicellulose; Mould, aspergillus niger of wood and aspergillus oryzae can be under acidic conditions Mierocrystalline cellulose and hemicellulose in (pH3-6) degraded xylose residue or the furfural dregs.In addition, this fungal inoculant can also destroy the physical structure of xylose residue or furfural dregs by the mechanical penetration power of mycelia, makes its easier degraded that becomes.Xylose residue or the furfural dregs handled by this composite fungi microbial inoculum are used for biogas fermentation, can accelerate biogas start time, and than untreated xylose residue or furfural dregs, can shift to an earlier date 3-10 days its start time usually, and gas producing efficiency improves 30%-60%.Especially in the winter time, xylose residue or the furfural dregs handled through the composite fungi microbial inoculum can guarantee as the biogas fermentation main raw material to continue aerogenesis under low temperature (15 ℃-18 ℃) condition, and control group adopts undressed xylose residue or furfural dregs to produce biogas hardly.
Embodiment
The present invention will be further described for following examples, but the present invention has more than and is limited to this.If no special instructions, the raw material consumption is a weight part.Xylose residue, furfural dregs, the peat composed of rotten mosses, diatomite can obtain by buying among each embodiment.Raw material needed 60 mesh sieves, and water content is below 10%.
Embodiment 1
Viride (
Trichoderma viride), Phanerochaete chrysosporium (
Phanerochaete chrysosporium), aspergillus niger (
Aspergillus niger), aspergillus oryzae (
Aspergillus oryzae), variable color bolt bacterium (
Trametes versicolor) all can obtain in Chinese common micro-organisms culture presevation administrative center (CGMCC), viride is CGMCC 3.5455,3.3711,3.4005,3.4264,3.2941,3.2942,3.2876,3.1913 one of them; Phanerochaete chrysosporium CGMCC is numbered 5.776, and variable color bolt bacterium CGMCC is numbered 5.48 or 5.161, and black-koji mould CGMCC is numbered 3.6479,3.6478,3.6477,3.6476,3.6475,3.6329 one of them; Aspergillus oryzae is 3.5232,3.4437,3.4383,3.4382,3.4259,3.2140 one of them.Behind the liquid pure-blood ferment of routine, obtain five kinds of bacterium liquid.Its dry cell weight all reaches 20 grams per liters.Specific implementation method is as follows:
1) adopts above-mentioned five kinds of microorganisms separately that the activated inclined plane substratum activates, under 30 ℃ of conditions, cultivated 8 days.
2) above-mentioned activation is good bacterial classification spore suspension is inoculated into liquid separately substratum.
Viride is cultivated: yeast extract paste 15 grams, glucose 20 grams, corn steep liquor 10 grams, wheat bran 20 grams, potassium primary phosphate 1.8 grams, sal epsom 0.9 gram, ammonium sulfate 6 grams, ferrous sulfate 0.05 gram, cobalt chloride 0.4 gram, calcium chloride 0.5 gram, 0.1 milliliter of defoamer, 1000 milliliters in tap water, pH5.0.Spore suspension (10
7Individual/milliliter) inoculum size 5%(v/v), 28 ℃ of culture temperature, 200 rev/mins of rotating speeds are cultivated 2 days to logarithmic phase acquisition liquid seeds.
Phanerochaete chrysosporium is cultivated: glucose 15 grams, potassium primary phosphate 3.6 grams, ammonium sulfate 2.4 grams, corn steep liquor 10 grams, wheat bran 5 grams, sal epsom 0.3 gram, calcium chloride 0.6 gram, 5.2 milligrams in ferrous sulfate, 1.8 milligrams of manganous sulfates, 2.2 milligrams in zinc sulfate, 0.8 milligram in copper sulfate, 1000 milliliters in tap water, pH 6.0.Spore suspension (10
6Individual/milliliter) inoculum size 5%(v/v), 28 ℃ of culture temperature, 150 rev/mins of rotating speeds are cultivated 3 days to logarithmic phase acquisition liquid seeds.
Variable color bolt bacterium is cultivated: glucose 15 grams, potassium primary phosphate 3.8 grams, ammonium sulfate 2.2 grams, corn steep liquor 12 grams, wheat bran 10 grams, sal epsom 0.3 gram, calcium chloride 0.6 gram, 4.6 milligrams in ferrous sulfate, 2.2 milligrams of manganous sulfates, 2.6 milligrams in zinc sulfate, 1.3 milligrams in copper sulfate, 1000 milliliters in tap water, defoamer 0.1ml, pH6.0.Spore suspension (10
6Individual/milliliter), inoculum size 5%(v/v), 28 ℃ of culture temperature, 200 rev/mins of rotating speeds, air flow 1:1(ventilation volume ratio is a benchmark with the fermentor tank liquid amount) cultivate 2 days to logarithmic phase acquisition liquid seeds.
Aspergillus oryzae is cultivated: glucose 10 grams, and starch 40, dregs of beans 30 grams, wheat bran 20 grams, potassium primary phosphate 1.5 grams, sal epsom 0.2 gram, ammonium sulfate 0.4 gram, calcium chloride 0.6g, Repone K 0.4g, 0.1 milliliter of defoamer, 1000 milliliters in tap water, pH 5.0.Spore suspension (10
7Individual/milliliter) inoculum size 10%(v/v), 30 ℃ of culture temperature, 200 rev/mins of rotating speeds, air flow 1:1(ventilation volume ratio is a benchmark with the fermentor tank liquid amount) cultivate 2 days to logarithmic phase acquisition liquid seeds.
3) seeding tank 300L, liquid amount 200L, feed intake respectively according to above-mentioned five kinds of microorganism culturing based formulas, and add the described defoamer of preamble in proportion, the sterilization back is inserted according to 5% inoculum size and is shaken a bottle liquid seeds liquid, trichoderma viride, black-koji mould and aspergillus oryzae incubation time 36 hours, 32 ℃ of culture temperature, rotating speed is 250 rev/mins; 28 ℃ of variable color bolt bacterium and Phanerochaete chrysosporium culture temperature, 200 rev/mins of rotating speeds, incubation time 3 days, 28 ℃ of culture temperature, 200 rev/mins of rotating speeds.Air flow is the 1:1(volume ratio in the above-mentioned fermenting process).
4) produce 2000 liters of fermentor tanks, 1400 liters of liquid amounts feed intake respectively according to above-mentioned five kinds of microorganism culturing based formulas, add defoamer sterilization back in proportion and reach the logarithmic phase liquid seeds according to the access of 10% inoculum size.Trichoderma viride, black-koji mould and aspergillus oryzae incubation time 32 hours, 32 ℃ of culture temperature, rotating speed is 300 rev/mins; Phanerochaete chrysosporium and variable color bolt bacterium incubation time 3 days, 28 ℃ of culture temperature, 200 rev/mins of rotating speeds.Air flow is the 1:1(volume ratio in the above-mentioned fermenting process).After finishing, fermentation obtains bacterium liquid.
Above-mentioned bacterium liquid mixes according to following ratio: trichoderma viride liquid 0.40, and Phanerochaete chrysosporium bacterium liquid 0.15, black-koji mould liquid 0.05, aspergillus oryzae liquid 0.08, variable color bolt bacterium bacterium liquid 0.10 promptly gets microbiobacterial agent.
Embodiment 2
The bacterium liquid and preparation method thereof of five kinds of bacterium is as described in the embodiment 1.
3 parts of xylose residues, 1 part in diatomite, 1 part of the peat composed of rotten mosses.Add microbiobacterial agent (trichoderma viride liquid 0.30, Phanerochaete chrysosporium bacterium liquid 0.10, black-koji mould liquid 0.06, aspergillus oryzae liquid 0.16, variable color bolt bacterium bacterium liquid 0.05 with mixing the back after xylose residue, diatomite and the peat composed of rotten mosses sterilization.), promptly get the composite solid microbial inoculum after mixing.
Embodiment 3
The preparation of the bacterium liquid of five kinds of bacterium is as described in the embodiment 1.
1 part of furfural dregs, 2 parts of the peats composed of rotten mosses, 2 parts in diatomite, the sterilization back adds mixing tank and adds microbiobacterial agent (trichoderma viride liquid 0.20, Phanerochaete chrysosporium bacterium liquid 0.15, black-koji mould liquid 0.05, aspergillus oryzae liquid 0.10, variable color bolt bacterium bacterium liquid 0.10.), promptly get the composite solid microbial inoculum after mixing.
Embodiment 4
The liquid bacterial agent that is obtained among the embodiment 1 is inoculated into (pH 3 in the xylose residue, water content 50%), inoculum size is by weight: 9 parts of xylose residues insert 1 part of liquid bacterial agent, after handling 15 days under 25 ℃ of conditions, carry out producing methane through anaerobic fermentation experiment (100 parts of pretreated xylose residues behind the mixing as main raw material, 0.1 part in urea), control group is 100 parts of untreated xylose residues, 0.1 part in urea.Under 35 ℃ of culture condition, biogas fermentation start time shortens 6 days than control group, and factor of created gase improves 45%.
Embodiment 5
With (pH 4 in the solid fungicide access furfural dregs that is obtained among the embodiment 2, water content 20%) and to regulate water content be 70%(w/w), after handling 20 days under 18 ℃ of conditions, carry out biogas fermentation experiment (100 parts of pretreated furfural dregs as main raw material, 0.1 part in urea), control group is 100 parts of untreated furfural dregs, 0.1 part in urea.Under 20 ℃ of culture condition, biogas fermentation start time shortens 10 days than control group, and factor of created gase improves 60%.
Claims (6)
1. xylose residue or furfural dregs pre-treatment microbial inoculum, be to be mixed and made into by the bacterium liquid that viride, Phanerochaete chrysosporium, aspergillus niger, aspergillus oryzae, five kinds of bacterium of variable color bolt bacterium obtain after liquid state fermentation, it is as follows by weight: trichoderma viride liquid 0.20-0.40, Phanerochaete chrysosporium bacterium liquid 0.10-0.15, black-koji mould liquid 0.05-0.10, aspergillus oryzae liquid 0.08-0.16, variable color bolt bacterium bacterium liquid 0.05-0.10; Above-mentioned fermented hypha weight dry weight is more than the 20g/L.
2. xylose residue according to claim 1 or furfural dregs pre-treatment microbial inoculum, the weight part that it is characterized in that the bacterium liquid of described five kinds of bacterium is a trichoderma viride liquid 0.40, Phanerochaete chrysosporium bacterium liquid 0.15, black-koji mould liquid 0.05, aspergillus oryzae liquid 0.08, variable color bolt bacterium bacterium liquid 0.10.
3. the preparation method of xylose residue as claimed in claim 1 or furfural dregs pre-treatment microbial inoculum, it comprises the steps: that 1. these five kinds of bacterium of viride, Phanerochaete chrysosporium, aspergillus niger, aspergillus oryzae and variable color bolt bacterium being produced five steps according to slant strains activation, shake flask fermentation, seeding tank liquid state fermentation, big jar liquid state fermentation respectively obtains trichoderma viride liquid, Phanerochaete chrysosporium bacterium liquid, black-koji mould liquid, aspergillus oryzae liquid, variable color bolt bacterium bacterium liquid respectively; 2. bacterium liquid is composite, and above-mentioned five kinds of bacterium bacterium liquid are mixed and made into liquid dosage form in proportion.
4. the preparation method of xylose residue according to claim 3 or furfural dregs pre-treatment microbial inoculum adds solid packing in the bacterium liquid after it is characterized in that trichoderma viride liquid, Phanerochaete chrysosporium bacterium liquid, black-koji mould liquid, aspergillus oryzae liquid, variable color bolt bacterium bacterium liquid is mixed in proportion and makes solid dosage.
5. the preparation method of xylose residue according to claim 4 or furfural dregs pre-treatment microbial inoculum, it is characterized in that described solid packing is one or more mixing in xylose residue, furfural dregs, the peat composed of rotten mosses, the diatomite, its total consumption is 4-10 times of bacterium liquid gross weight after mixing.
6. the purposes of xylose residue or furfural dregs pre-treatment microbial inoculum is characterized in that described xylose residue of claim 1 or furfural dregs pre-treatment microbial inoculum are used for xylose residue or the pre-treatment of furfural dregs before carrying out biogas fermentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100212124A CN102154108A (en) | 2011-01-19 | 2011-01-19 | Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100212124A CN102154108A (en) | 2011-01-19 | 2011-01-19 | Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102154108A true CN102154108A (en) | 2011-08-17 |
Family
ID=44435784
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100212124A Pending CN102154108A (en) | 2011-01-19 | 2011-01-19 | Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102154108A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102557767A (en) * | 2011-12-19 | 2012-07-11 | 西华大学 | Special compound fungus agent for humification of fungus residues of straw mushrooms and manufacturing method thereof |
| CN102874912A (en) * | 2011-12-31 | 2013-01-16 | 安徽理工大学 | Composite bacterial coal biological flocculant and method for purifying slime water by same |
| CN103923843A (en) * | 2014-04-22 | 2014-07-16 | 山东省科学院能源研究所 | Method for pre-treating corn cob residue by using fungi to improve quality of activated carbon |
| CN105647813A (en) * | 2014-12-05 | 2016-06-08 | 中国石油化工股份有限公司 | Trichoderma viride strain, and applications thereof |
| CN106721891A (en) * | 2016-12-23 | 2017-05-31 | 五河县盛源畜牧养殖专业合作社 | A kind of poultry greenfeed processing harmful bacteria growth inhibition additive |
| CN107417415A (en) * | 2017-09-01 | 2017-12-01 | 济南圣泉集团股份有限公司 | A kind of agricultural microbial agent and preparation method thereof |
| CN107746303A (en) * | 2017-10-18 | 2018-03-02 | 济南圣泉集团股份有限公司 | It is a kind of using xylose residue as decomposed matrix of raw material and its preparation method and application |
| WO2019094995A1 (en) * | 2017-11-17 | 2019-05-23 | STIMPFL, Georg | Method for utilizing biomass |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888073A (en) * | 2005-06-27 | 2007-01-03 | 北京合百意生态能源科技开发有限公司 | Marsh gas fermentation process with composite microbial prepn pre-treated stalk |
| CN101372666A (en) * | 2008-10-17 | 2009-02-25 | 山东省农业科学院土壤肥料研究所 | Composite microbial preparation for methane tank and preparation thereof |
| CN101827939A (en) * | 2007-08-30 | 2010-09-08 | 埃欧金能源公司 | Enzymatic hydrolysis of lignocellulosic feedstocks using accessory enzymes |
-
2011
- 2011-01-19 CN CN2011100212124A patent/CN102154108A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888073A (en) * | 2005-06-27 | 2007-01-03 | 北京合百意生态能源科技开发有限公司 | Marsh gas fermentation process with composite microbial prepn pre-treated stalk |
| CN101827939A (en) * | 2007-08-30 | 2010-09-08 | 埃欧金能源公司 | Enzymatic hydrolysis of lignocellulosic feedstocks using accessory enzymes |
| CN101372666A (en) * | 2008-10-17 | 2009-02-25 | 山东省农业科学院土壤肥料研究所 | Composite microbial preparation for methane tank and preparation thereof |
Non-Patent Citations (3)
| Title |
|---|
| 《中国优秀硕士学位论文全文数据库 农业科技辑》 20100615 邵喜霞 秸秆降解菌的诱变筛选及降解效果研究 , * |
| 《中国优秀硕士学位论文全文数据库 农业科技辑》 20110115 刘甲锋 水稻秸秆腐解复合菌系的筛选构建 , * |
| 《林业科学》 20060330 张晓燕等 木质生物质的生物分解及生物转化研究进展 , 第03期 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102557767A (en) * | 2011-12-19 | 2012-07-11 | 西华大学 | Special compound fungus agent for humification of fungus residues of straw mushrooms and manufacturing method thereof |
| CN102557767B (en) * | 2011-12-19 | 2014-07-02 | 西华大学 | Special compound fungus agent for humification of fungus residues of straw mushrooms and manufacturing method thereof |
| CN102874912A (en) * | 2011-12-31 | 2013-01-16 | 安徽理工大学 | Composite bacterial coal biological flocculant and method for purifying slime water by same |
| CN102874912B (en) * | 2011-12-31 | 2013-11-06 | 安徽理工大学 | Composite bacterial coal biological flocculant and method for purifying slime water by same |
| CN103923843B (en) * | 2014-04-22 | 2016-08-24 | 山东省科学院能源研究所 | The method utilizing fungus pretreatment xylose residue for improving activated carbon quality |
| CN103923843A (en) * | 2014-04-22 | 2014-07-16 | 山东省科学院能源研究所 | Method for pre-treating corn cob residue by using fungi to improve quality of activated carbon |
| CN105647813A (en) * | 2014-12-05 | 2016-06-08 | 中国石油化工股份有限公司 | Trichoderma viride strain, and applications thereof |
| CN105647813B (en) * | 2014-12-05 | 2019-05-21 | 中国石油化工股份有限公司 | One plant of Trichoderma viride and its application |
| CN106721891A (en) * | 2016-12-23 | 2017-05-31 | 五河县盛源畜牧养殖专业合作社 | A kind of poultry greenfeed processing harmful bacteria growth inhibition additive |
| CN107417415A (en) * | 2017-09-01 | 2017-12-01 | 济南圣泉集团股份有限公司 | A kind of agricultural microbial agent and preparation method thereof |
| CN107746303A (en) * | 2017-10-18 | 2018-03-02 | 济南圣泉集团股份有限公司 | It is a kind of using xylose residue as decomposed matrix of raw material and its preparation method and application |
| WO2019094995A1 (en) * | 2017-11-17 | 2019-05-23 | STIMPFL, Georg | Method for utilizing biomass |
| AT520801A1 (en) * | 2017-11-17 | 2019-07-15 | Georg Stimpfl | Process for the utilization of biomass |
| AT520801B1 (en) * | 2017-11-17 | 2021-01-15 | Georg Stimpfl | Process for the utilization of biomass |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sindhu et al. | Water hyacinth a potential source for value addition: an overview | |
| CN105567612B (en) | A kind of degradation composite bacteria agent preparation of garden waste and application | |
| CN102154108A (en) | Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof | |
| CN101974436B (en) | Lignocellulose degrading bacteria and application thereof | |
| CN106631248A (en) | Production process for bioorganic fertilizer with high bacteria content | |
| CN102453676B (en) | Straw starter and use of the straw starter in straw fermentation | |
| CN101591679B (en) | Method for improving utilization rate in native grass-based fuel ethanol production by using mixed strains | |
| CN101100660B (en) | Method for producing cellulose by microorganism mixing fermentation | |
| CN103694010A (en) | Ultrahigh-temperature aerobic fermentation method for sludge and application thereof | |
| CN103642726A (en) | Corn straw fermentation microorganism agent and preparation method and fermentation method thereof | |
| CN104692866A (en) | Biological organic fertilizer | |
| CN101638673B (en) | Method for manufacturing alcohol by utilizing fermentation of plant straws | |
| CN105948841B (en) | Organic fertilizer tank type fermentation method taking mushroom dregs as substrate | |
| CN105002221A (en) | Method for producing biogas through high-efficiency anaerobic fermentation of organic waste resource in intensive vegetable area | |
| CN102174423B (en) | Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof | |
| CN106811438B (en) | Straw degradation acidification microbial inoculum and preparation method thereof | |
| CN104692844A (en) | Preparation method of bioorganic fertilizer | |
| CN111363684A (en) | Composite microbial inoculum for efficiently degrading wood fibers and application thereof in composting | |
| CN101696391B (en) | Rapid composting microbial inoculum of agricultural wastes and method for preparing organic fertilizer from the same | |
| CN101864363B (en) | Complex bacterial preparation and application thereof | |
| CN111334452A (en) | Compound bacterium agent for degrading livestock and poultry manure and application thereof | |
| CN103012009A (en) | Organic acid plant soil conditioner and preparation method thereof | |
| Smith et al. | Bioprocessing of lignocelluloses | |
| CN103074378B (en) | Composite biological preparation for biogas fermentation | |
| CN1326809C (en) | Refuse composting process adding different decay promoting ferments separately and its decay promoting ferments |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110817 |