CN101975898B - Device for dynamically and electrically researching dielectric property of cells by using ultrasonic pulse - Google Patents
Device for dynamically and electrically researching dielectric property of cells by using ultrasonic pulse Download PDFInfo
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- CN101975898B CN101975898B CN2010102844919A CN201010284491A CN101975898B CN 101975898 B CN101975898 B CN 101975898B CN 2010102844919 A CN2010102844919 A CN 2010102844919A CN 201010284491 A CN201010284491 A CN 201010284491A CN 101975898 B CN101975898 B CN 101975898B
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Abstract
The invention discloses a device for dynamically and electrically researching dielectric property of cells by using ultrasonic pulse. The device comprises a computer, an acquisition card and a support with a vertical liquid accommodating cavity; an ultrasonic transducer is arranged in the liquid accommodating cavity of the support; an ultrasonic reflection preventing sound absorption material is filled between the lower end of the ultrasonic transducer and the bottom of the liquid accommodating cavity; a cavity for accommodating a cell suspension sample is positioned above the ultrasonic transducer in the liquid accommodating cavity; a sampling electrode in liquid level contact with the cell suspension sample is arranged at the upper end opening of the liquid accommodating cavity, the sampling electrode is connected with a micro current detection circuit, and the micro current detection circuit is connected to the computer through the acquisition card; and two electrodes of the ultrasonic transducer are connected with ultrasonic signal generating sources. The device can realize research of real-time ultrasonic biological effect under the action of ultrasonic according to a dynamic electric theoretical model of cell suspension by continuously adjusting the functional state of the ultrasonic; and the device has a simple structure and good repeatability, is easy to operate, and has no damage to the cells.
Description
Technical field
The invention belongs to biological cell fundamental research field; Relate to a kind of device of studying the cell dielectric characteristic; Especially a kind of device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic; It moves electric theory model from electric charge level research cell dielectric characteristic as sonic probe according to suspension cell with ultrasonic pulse, can be used for the research of cell membrane surface charging characteristic aspect, especially cell biological effect of ultrasound aspect is studied in ultrasonication down in real time and is had inborn superiority.
Background technology
The ultrasound wave irradiation of doses can cause multiple biological effect in the biosystem of varying level.Up to the present, under many kinds of situation, the physical mechanism of biological effect of ultrasound is still very unclear, does not more have a kind of suitable method can study the process of biological effect of ultrasound in real time.At present, mainly contain methods such as cell electrophoresis method, electrochemical impedance method, patch clamp technique to cell dielectric The Characteristic Study method.The cell electrophoresis method is under the extra electric field effect; Cell is done distinctive moving according to the difference of its surface charge, size, shape; Detect the method for cell membrane electrical characteristics according to the information of the electronic characteristic aspects such as electrophoretic mobility of cell, mainly there are shortcomings such as more numerous, required long period of operation, repeatability and sensitivity is relatively poor in it.The electrochemical impedance method is that a kind of electricity with little amplitude is pulsed as the electrochemical impedance measuring method of disturbing signal; In addition, it also can study electrode system with the very wide impedance spectrum of measurement range, thereby obtains cell electrodynamics information, and studies the method for cell membrane charge characteristic with this, and there are shortcomings such as complicated operation, repeatability and low precision equally in this method.Patch clamp technique is to obtain the technology of electronics aspect information from small pieces (a few approximately square micron) film; Promptly keep transmembrane voltage constant-voltage clamp; Thereby measure technology through cell membrane gas current size, though this method precision is very high, apparatus expensive, complicated operation.In addition, all methods all can not be studied the biological effect of ultrasound of cell in real time more than under ultrasonication.
When ultrasonic (the alternating pressure field) of a high frequency passed a colloid system; The density of medium around wherein the density of colloidal particle is different from; The inertial force that the vibration of suspension causes causes charged particle to follow its motion, thereby causes the electrostatic double layer on micelle surface to produce distortion.Fixed bed and diffusion layer relatively move in the micelle surface electric double layer, and the change of the zeta potential that shows can be detected by outer electrode, and the current potential of this variation is called colloid vibration current potential (CVP); Simultaneously, if external circuit is closed, owing to ultrasonic passing through, will produce the electric current of an alternation in the system, the electric current of this alternation is called as (CVI).Same electroacoustic phenomenon also can appear in the electrolyte solution.Because negative ion is different each other with cationic group in the solution, therefore they will produce periodically dislocation under ultrasonic effect, thereby oscillating current occurs.This electric current is called as ion oscillating current (IVI).Though to first time of this electroacoustic phenomenon report is to propose prediction in 1933 by the debye, up to the nineties in last century Mike O'Brien just set forth his theory, thereby make this electroacoustic techniques that tremendous development arranged and be used in the business equipment.The moving topmost advantage of electrical measurement method is the original strong solution that need not dilute of its energy measurement, even the energy measurement plot ratio reaches 50% solution, and this point makes its be fit to very much many dense biosystems.And in this measuring system, the magnitude range of micelle can be from 100nm to 10 μ m, and this makes it very be fit to size probably is the human red cells of 6 μ m to 9 μ m.The most promising purposes of this technology is exactly medical early diagnosis and the research of cell biological effect of ultrasound in real time, and can be further used for the ultrasonic imaging of unicellular characteristic research.
Up to the present; The research of the moving electric theory method of colloid and application are mainly at chemical field; Basically all be measurement to colloidal suspension particle zeta potential; These particles mainly all are ganoid polymkeric substance grits, the soft particle that ion can penetrating high molecular polymer yet cell but is a surface coverage.In addition, in the moving electric theory model of colloid in the past, all be to use continuous ultrasound wave as vibration source, be not suitable for biological cell is studied, because be approximately 10mw/cm at the sound intensity
2The time, under the ultrasound wave effect, cell has begun to produce biological effect of ultrasound, causes testing result inaccurate; And the low sound intensity acts in the cell suspension, is unlikely to make a little less than distortion of cell electrostatic double layer or the distortion very, makes to detect the unusual difficulty that becomes.So use ultrasonic pulse among the present invention, can single detection ultrasonic pulse energy be increased, thereby detection signal is increased as probe; But the equal sound intensity of whole ultrasonic popin is reduced, so that it can not make cell produce biological effect.In addition, also can detect pulse, thereby reach the function of real-time research cell biological effect of ultrasound through between continuous function property ultrasound wave, inserting.
At present, the model that ion can penetrating flexible glue grain has both at home and abroad just been proposed in theory.According to the model of Debye-Bueche and the acoustic theory of flexible glue grain suspension; Electric current summation (TVI) in the cell suspension can be expressed as ion oscillating current (IVI) and cell oscillating current (CVI) sum; Be shown below, but do not propose correlation theory model and its implementation that actual cell suspension moves electric theory.
TVI＝IVI+CVI (1)
Summary of the invention
The objective of the invention is to overcome the shortcoming of above-mentioned prior art, a kind of device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic is provided, this device is according to the moving electric theory model of cell suspension; Can be through adjusting ultrasonic functional status continuously; Thereby realize that this device is not only simple in structure, and processing ease to real-time ultrasound Biological effects under the ultrasonication; Good reproducibility, and not damage of pair cell.
The objective of the invention is to solve through following technical scheme:
This device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic; Comprise computing machine and capture card; Also include bearing, be provided with ultrasonic transducer in the Sheng sap cavity of said bearing, be filled with the acoustic absorbant that prevents ultrasonic reflection between the lower end of said ultrasonic transducer and the Sheng sap cavity bottom with vertical Sheng sap cavity; Said Sheng sap cavity is interior to be the cavity that holds the cell suspension sample in the top of ultrasonic transducer; The open upper end place of said Sheng sap cavity is provided with the sampling electrode that contacts with cell suspension sample liquid level, and said sampling electrode is connected with micro-current sensing circuit, and said micro-current sensing circuit is connected on the computing machine through capture card; Two electrodes of said ultrasonic transducer connect ultrasonic signal the source take place.
The source takes place and is connected and composed successively by high capacity cadmium-nickel storage cell, ultrasonic waveform control circuit and ultrasonic generation circuit in above-mentioned ultrasonic signal; Said ultrasonic waveform control circuit produces the pulse electrical signal of turnable pulse width; This pulse electrical signal is through the grid source electrode of control IGBT; Regulate the waveform of ultrasonic signal, make ultrasonic signal become pulse waveform by continuous wave; Said ultrasonic generation circuit produce adjustable amplitude and with the consistent sinusoidal signal of said ultrasonic transducer centre frequency, the IGBT copped wave that this sinusoidal signal is controlled through ultrasonic waveform control circuit finally converts the pulse electrical signal that is applied on the ultrasonic transducer into.
Above-mentioned ultrasonic waveform control circuit comprises the source of stable pressure circuit and based on the signal generating circuit of signal generation chip MAX038; Said source of stable pressure circuit is that said signal generating circuit provides burning voltage.
Further, above-mentioned bearing has the steel counterweight, and the Sheng sap cavity of bearing is made by teflon.
Further, above-mentioned sampling electrode is inert electrode or gold-plated electrode.
Above-mentioned acoustic absorbant is a polymethylmethacrylate.
Above-mentioned ultrasonic transduction is the silver-plated lead zirconate titanate ultrasonic transducer in two sides, and the said ultrasonic transducer wherein silver electrode of one side extends to the another side edge, and draws lead; The another side electrode is drawn lead from the silver electrode center.
The edge of above-mentioned ultrasonic transducer is by the rubber sleave sealing of smearing vaseline.
The present invention has following beneficial effect:
The device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic of the present invention can realize that the pair cell dielectric property studies; And the surface charge that can under ultrasonication, detect cell changes; Thereby reach the function of the biological effect of ultrasound of real-time research cell; And apparatus structure of the present invention is simple, good reproducibility, and pair cell has no damage.
Description of drawings
Fig. 1 is ultrasonic waveform control circuit figure;
Fig. 2 is ultrasonic generation circuit diagram;
Fig. 3 is that the ultrasonic transducer sound intensity is demarcated block diagram;
Fig. 4 is the theory diagram of the utility model pick-up unit;
Fig. 5 is micro-current sensing circuit figure among Fig. 4.
Embodiment
Below in conjunction with accompanying drawing the present invention is done and to describe in further detail:
Referring to Fig. 4; The present invention utilizes the device of the moving electricity research of ultrasonic pulse cell dielectric characteristic; Comprise computing machine, capture card and bearing, in the Sheng sap cavity of bearing, be provided with ultrasonic transducer, be filled with the acoustic absorbant that prevents ultrasonic reflection between the lower end of this ultrasonic transducer and the Sheng sap cavity bottom with vertical Sheng sap cavity; In the optimal case of the present invention, it is PMMA that acoustic absorbant is selected polymethylmethacrylate for use.It in containing sap cavity the cavity that holds the cell suspension sample; The open upper end place of containing sap cavity is provided with the sampling electrode that contacts with cell suspension sample liquid level; The sampling electrode contacts with the sample liquid level fully, and to reduce sampling error, this sampling electrode is connected with micro-current sensing circuit; Micro-current sensing circuit is used for accurately detecting the moving electric signal of cell; Micro-current sensing circuit is connected on the computing machine through capture card, and capture card is used for acquisition testing signal and be transferred to computing machine, carries out wave form analysis.Two electrodes of above-mentioned ultrasonic transducer are connected with ultrasonic signal the source take place.Below the each several part that constitutes apparatus of the present invention is described in detail:
Bearing
Bearing shape of the present invention can design as required, has down the bearing in " protruding " font chamber like inside among Fig. 4, and the steel counterweight wherein is set on bearing, and the Sheng sap cavity of bearing made by teflon, so that cavity has strong corrosion resistance.
The sampling electrode
The sampling electrode must use inert electrode or gold-plated electrode, thereby prevents the corrosion of electrode.
Ultrasonic transducer
In preferred embodiment of the present invention, ultrasonic transduction is the silver-plated lead zirconate titanate high-performance ultrasonic transducer in two sides, uses centre frequency to be 1.7MHz.This ultrasonic transducer wherein silver electrode of one side extends to the another side edge, and draws lead; The another side electrode is drawn lead from the silver electrode center.The edge of this ultrasonic transducer is sealed by the rubber sleave of smearing vaseline, thereby prevents ultrasonic transducer short circuit in cell suspension.This ultrasonic transducer be encapsulated into must use before containing sap cavity phonometer to two electrode application voltage of ultrasonic transducer and output sound intensity between corresponding relation accurately demarcate; Thereby could Accurate Analysis the moving electrical characteristics of cell, the mode of specifically demarcating is referring to Fig. 3, among the figure in ultrasonic transducer sound intensity calibration process; Ultrasonic through the transmission of hydrophily matter; Ultrasonic probe and ultrasonic transducer place the cylindricality water receptacle, are protected by bucker around the ultrasonic transducer, to prevent transducer two electric pole short circuits; Wherein bearing and support play the effect of stable support; Ultrasonic signal generation source is the circuit among Fig. 1 and Fig. 2; Ultrasonic probe is conical Vibration induction probe, and it is transferred to the vibrations power meter with induced signal, and shows output.
The source takes place in ultrasonic signal
The source takes place and is connected and composed successively by high capacity cadmium-nickel storage cell, ultrasonic waveform control circuit and ultrasonic generation circuit in ultrasonic signal.Described ultrasonic waveform control circuit produces the pulse electrical signal of turnable pulse width, and this pulse electrical signal is regulated the waveform of ultrasonic signal through the grid source electrode of control IGBT, makes ultrasonic signal become pulse waveform by continuous wave; Described ultrasonic generation circuit produce adjustable amplitude and with the consistent sinusoidal signal of said ultrasonic transducer centre frequency; This sinusoidal signal finally converts the pulse electrical signal that is applied on the ultrasonic transducer into through the IGBT copped wave of ultrasonic waveform control circuit control.In preferred embodiment of the present invention, the concrete structure or the parameter information that more than constitute the each several part in ultrasonic signal generation source are described below:
(1) high capacity cadmium-nickel storage cell: the present invention uses the high capacity cadmium-nickel storage cell can guarantee that ultrasonic signal takes place that the source is sustainable to work long hours, and through use chemical cell can be to greatest extent with the power supply in the ultrasonic signal generation source disturb drop to minimum.
(2) ultrasonic waveform control circuit: referring to Fig. 1, this ultrasonic waveform control circuit comprises source of stable pressure circuit 101, based on signal generating circuit 102 and the IGBT driving circuit 103 of signal generation chip MAX038; Said source of stable pressure circuit 101 provides burning voltage for said signal generating circuit 102 and IGBT driving circuit 103; The signal output part of said signal generating circuit 102 is connected to the IGBT grid and the source electrode (like Fig. 2) of ultrasonic generation circuit through IGBT driving circuit 103, and physical circuit figure introduces as follows:
In the source of stable pressure circuit of Fig. 1, J1 and J2 are the connector head; S1 and S2 are power switch; D1 is a reversal of power protection diode; Z1 and Z2 are respectively voltage stabilizing chip LM7805 and LM7905, respectively with stabilized input voltage at+5V and-5V; D2 and D3 are chip reverse protection diode, are used for protecting the voltage stabilizing chip; E1, E2, C1, C2 are filter capacitor, low-and high-frequency clutter in the filter out power; D5 and D6 are voltage stabilizing diode, stable respectively+5V and-5V, protection power supply; DS1 and DS2 are light emitting diode, are used for indicating power supply to cut-off; Wherein network mark+5V is connected network mark+5V and network mark-5V in the latter half circuit diagram respectively with network mark-5V.
In the signal generating circuit of Fig. 1 based on signal generation chip MAX038; The pin two 0 of chip MAX038 connects the network mark-5V end of source of stable pressure circuit; The pin one 7 of chip MAX038 connects the network mark+5V end of source of stable pressure circuit; They reduce power supply with this and disturb respectively through capacitor C 3 and C4 ground connection; The pin two of chip MAX038,6,9,11,12,13,15,18 connects the network mark GND end of source of stable pressure circuit, i.e. ground connection; The pin 8 of chip MAX038 is through resistance R 3 ground connection; The pin one of chip MAX038 connects the pin one 0 of chip MAX038 through resistance R W; And the pin one of chip MAX038 is connected the pin 7 of chip MAX038 with capacitor C 4 through variable resistor RDAJ; Wherein RDAJ is a slide rheostat, is may command output waveform dutycycle through changing the RDAJ terminal potential; Wherein S2-S7 is a frequency band conversion toggle switch; Their control end is connected the pin 5 of chip MAX038; Common port is connected the power supply ground end of chip MAX038, and the controlled frequency section is 1-100Hz, 100Hz-1KHz, 1KHz-10KHz, 10KHz-100KHz, 100KHz-1MHz, 1MHz-10MHz respectively; Wherein toggle switch S8 one end connects the pin 4 or 17 of chip MAX038, and other end ground connection is controlled the type of output signal with this.
In this signal generating circuit, bottom right half partial circuit figure is the signal amplification circuit at center and is the signal drive circuit at center with triode Q1 with amplifier OP07C.Wherein the signal input part 2 of amplifier OP07C is through the pin one 9 of the chip MAX038 that is connected in series of repeating resistance R3 and input resistance R4; Feedback resistance R6 connects the negative input end 2 and output terminal 3 of OP07C, the enlargement factor of coming control circuit through the ratio of regulating resistance R 6 and resistance R 5; Wherein R7 is the amplifier build-out resistor, is connected between the positive input terminal 3 and ground of amplifier OP07C; Capacitor C 6 is respectively power filtering capacitor with C7, be serially connected in respectively amplifier OP07C positive-negative power end 4 and 7 and ground between.Capacitor C 14 constitutes low frequency filter with resistance R 8 serial connections, and it connects the output terminal 6 of amplifier OP07C and the base terminal of triode Q1; Terminal J3 connects driving power, through diode D4 and D7 protection, is connected the collector terminal of triode Q1; J4 is a lead-out terminal, is connected on the emitter of triode Q1, the ultrasonic waveform control circuit Y1 output terminal 3 and 4 in its connection layout 2; R9 and R11 are divider resistance; DS3 is the output signal lamp.
(3) ultrasonic generation circuit: Fig. 2 is the circuit diagram of ultrasonic generation circuit, in Fig. 2, and the ultrasonic waveform control circuit in the Y1 representative graph 1, this circuit is connected with ultrasonic generation circuit.In the ultrasonic generation circuit in the drawings, QQ1 is that model is the IGBT of FGA25N120AN, and its two poles of the earth, grid source connect the output terminal 3 and 4 of Y1 respectively; Its drain electrode connects the negative pole of power supply BT, controls the conducting of power supply BT with this, through regulating electric signal dutycycle and frequency among the Y1, controls the waveform of ultrasonic signal, and this IGBT can tolerate 1200V voltage and 25A electric current, can satisfy the circuit needs.LM338 is the adjustable voltage stabilizing chip of 5A, and its input end Vi connects the positive pole of power supply BT; Adjustment end ADJ is through slide rheostat RR1 ground connection; Output end vo connects the ADJ end through resistance R R2; Wherein RR1 and RR2 are that output voltage is regulated resistance; May command is added in the voltage of ultrasonic transducer, thereby controls hyperacoustic intensity, and its formula is
through regulating the RR2 resistance; Can realize that the function that the source output voltage is regulated takes place ultrasonic signal; This chip direct current can be crossed the 5A electric current, but and extra pulse 7A electric current, satisfy matching requirements; CC1, CC2 are filter capacitors, but the high frequency interference of filter out power.SS1 is the ultrasonic-frequency power supply switch, controls ultrasonic cut-offfing, and it is serially connected in the output terminal of LM338; DD1 is a power light, and RR3 is the pilot lamp current-limiting resistance, and they are connected in parallel on the output terminal of LM338.
The oscillator that ultrasonic generation circuit connects is a kind of oscillator of being made up of high frequency piezoelectric ceramic sheet YD (being ultrasonic transducer), and its oscillation frequency is 1.7MHz (resonance frequency of the YD that is decided by to select).Model is that the collector of the transistor QQ2 of BU406 is connected the collector that model is the transistor QQ3 of 2SC1815 through resistance in series RR4 and RR5; The emitter tandem electric inductance L1 of triode QQ2; The base stage of triode QQ2 connects the emitter of triode QQ3, and is connected the common port of inductance L 1 and L2 through serial connection capacitor C C4; Inductance L 2 one ends connect inductance L 1, and the other end connects the ground electrode of Y1; Capacitor C C3 one end connects the collector of triode QQ2, the common port of another termination inductance L 1 and L2; Triode QQ3 base stage series resistor RR6 connects the collector of triode QQ2, and this common port is connected ultrasonic transducer YD input end P, and the emitter serial connection capacitor C C5 of triode QQ3 connects ultrasonic transducer input end Z.Triode QQ2 and capacitor C C3, CC4 have constituted Colpitts oscillation circuit in the circuit.The resonance frequency of capacitor C C3 and inductance L 1 equivalent parallel is lower than frequency of operation, and its effect is the oscillation amplitude of decision work vibration; The resonance frequency of capacitor C C4 and inductance L 2 equivalent series is higher than frequency of operation; Its effect is the feedback quantity of decision oscillator; To guarantee the reliable vibration of oscillator starting of oscillation and holding circuit; Piezoelectric ceramic piece YD has very big equivalent capacity, and it is again the operating load in the circuit except that the frequency of operation of decision-making circuit simultaneously.If change ultrasonic transducer YD, need not to adjust other parameters of circuit, its oscillation frequency also can be worked from the frequency of the new piezoelectric ceramic piece of motion tracking.Power supply provides bias current for triode QQ2 through resistance R R4 and RR5, triode QQ3 and resistance R R6, makes its ability oscillatory work.
Micro-current sensing circuit
Fig. 5 is micro-current sensing circuit figure among Fig. 4, and wherein left-half is a power supply, BT1 and BT2 be respectively+and 12V and-12V Ni-MH battery; DS11 and DS22 are power light.A1 is high speed, low-leakage current amplifier chip OPA637 in the right-hand part circuit, and its 7 ends connect network mark+12V, and 4 ends connect network mark-12V, and 3 ends are connected ground electrode with 8 ends; Slide rheostat W11 is connected between the offset side 1 and 5 of amplifier chip A1, and its control end is connected 4 ends of amplifier chip A1, is used to modulate bias current; Sampling resistor RC is parallelly connected with filter capacitor CA, and they are serially connected in 2 ends and 6 ends of amplifier chip A1, is used for sampling; IN is a signal input part, is connected 2 ends of amplifier chip A1; OUT is a signal output part, is connected 6 ends of amplifier chip A1.
In sum, ultrasonic waveform control circuit of the present invention produces turnable pulse width, the maximum peak peak value is the pulse electrical signal of 10V, and this signal is regulated the waveform of ultrasonic signal through the grid source electrode of control IGBT, makes ultrasonic signal become pulse waveform by continuous wave.Ultrasonic generation circuit produces adjustable amplitude and the sinusoidal signal consistent with the ultrasonic transducer centre frequency, and this sinusoidal signal finally converts the pulse electrical signal that is applied on the ultrasonic transducer into through the control of the IGBT of ultrasonic waveform control circuit control.In micro-current sensing circuit, at a high speed, low-leakage current amplifier chip OPA637 played key effect, its gain bandwidth (GB) can reach 80MHz; And in circuit, used the sampling resistor of 100M Ω, therefore can obtain concerning between TVI and the output voltage, as shown in the formula:
TVI＝10
-8U (2)
In device, can apply voltage magnitude through outside in the change ultrasonic signal generation source and change the voltage magnitude that is applied on the ultrasonic transducer, thus the control ultrasonic pulse sound intensity.In addition, use the ultrasonic transducer of centre frequency in the device,, thereby obtain the ultrasonic pulsative signal that repetition frequency is 1kHz through the pulsewidth of electric impulse signal in the adjusting ultrasonic signal generation source as 1.7MHz.Because whole charge migration amount that ultrasonic pulse causes is approximately Δ Q, as shown in the formula:
ΔQ＝IVI
Average×Δt (3)
Here Δ t is the detection time of a ultrasonic pulse, is 25 μ s in the device.IVI
AverageBe the mean value of IVI in the ultrasonic pulse testing process.According to formula (1), can accurately on charge level, study the moving electrical characteristics of cell.With formula (1) substitution formula (3), can obtain in the ultrasonic pulse testing process all that the charge migration amounts are ionic charge migration amount and cell charge migration amount sum, as shown in the formula:
ΔQ＝IVI
Average×Δt+CVI
Average×Δt＝ΔQ
ion+ΔQ
cell (4)
Use the hypervelocity capture card of SF in the device, thereby improve accuracy of detection, after data are adopted capture card,, through data processing software in the computing machine, handle, show and analysis waveform through USB input computing machine as 50MHz.
Data transmission is all used shielding line in the above experimental provision; Joint all uses BNC head or USB interface; Micro-current sensing circuit uses the shielding box shielding separately, and uses the cadmium-nickel storage cell power supply separately, and all devices is inserted in the complete shielded metal cabinet; The metal cabinet is arranged reliable ground through copper cash, thereby pick-up unit and external disturbance are isolated fully.
Claims (7)
1. one kind is utilized the moving electric device of studying the cell dielectric characteristic of ultrasonic pulse; Comprise computing machine and capture card; It is characterized in that: also comprise bearing, be provided with ultrasonic transducer in the Sheng sap cavity of said bearing, be filled with the acoustic absorbant that prevents ultrasonic reflection between the lower end of said ultrasonic transducer and the Sheng sap cavity bottom with vertical Sheng sap cavity; Said Sheng sap cavity is interior to be the cavity that holds the cell suspension sample in the top of ultrasonic transducer; The open upper end place of said Sheng sap cavity is provided with the sampling electrode that contacts with cell suspension sample liquid level, and said sampling electrode is connected with micro-current sensing circuit, and said micro-current sensing circuit is connected on the computing machine through capture card; Two electrodes of said ultrasonic transducer connect ultrasonic signal the source take place; The source takes place and is connected and composed successively by high capacity cadmium-nickel storage cell, ultrasonic waveform control circuit and ultrasonic generation circuit in said ultrasonic signal; Said ultrasonic generation circuit comprises IGBT (QQ1), power supply (BT), ultrasonic modulation circuit (104) and the oscillatory circuit (105) of mutual series connection; Said ultrasonic waveform control circuit produces the pulse electrical signal of turnable pulse width; This pulse electrical signal is through the grid source electrode of the IGBT (QQ1) of the ultrasonic generation circuit of control; Regulate the waveform of ultrasonic signal, make ultrasonic signal become pulse waveform by continuous wave; Said ultrasonic generation circuit produce adjustable amplitude and with the consistent sinusoidal signal of said ultrasonic transducer centre frequency, the IGBT that this sinusoidal signal is controlled through ultrasonic waveform control circuit finally converts the electric impulse signal that is applied on the ultrasonic transducer into.
2. the device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic according to claim 1 is characterized in that: said ultrasonic waveform control circuit comprises source of stable pressure circuit (101), based on signal generating circuit (102) and the IGBT driving circuit (103) of signal generation chip MAX038; Said source of stable pressure circuit (101) provides burning voltage for said signal generating circuit (102) and IGBT driving circuit (103); The signal output part of said signal generating circuit (102) is connected to ultrasonic generation circuit through IGBT driving circuit (103).
3. the device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic according to claim 1, it is characterized in that: said bearing has the steel counterweight, and the Sheng sap cavity of bearing is made by teflon.
4. the device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic according to claim 1, it is characterized in that: said sampling electrode is an inert electrode.
5. the device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic according to claim 1, it is characterized in that: said acoustic absorbant is a polymethylmethacrylate.
6. the device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic according to claim 1; It is characterized in that: said ultrasonic transducer is the silver-plated lead zirconate titanate ultrasonic transducer in two sides; The said ultrasonic transducer wherein silver electrode of one side extends to the another side edge, and draws lead; The another side electrode is drawn lead from the silver electrode center.
7. the device that utilizes the moving electricity research of ultrasonic pulse cell dielectric characteristic according to claim 6, it is characterized in that: the edge of said ultrasonic transducer is by the rubber sleave sealing of smearing vaseline.
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|---|---|---|---|---|
| CN1260486A (en) * | 1999-02-23 | 2000-07-19 | 王劲松 | Detecting method and apparatus for yeast concentration (measuring bioconcentration) |
| CN1906485A (en) * | 2003-11-26 | 2007-01-31 | 分离技术公司 | Method and apparatus for ultrasonic determination of hematocrit and hemoglobin concentrations |
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| 吴超一等.脉冲电声法空间电荷测量波形恢复的数据处理.《中国电机工程学报》.2005,第25卷(第16期),137－140. * |
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