CN101904876A - Method for extracting total triterpenoids in lucid ganoderma as lucid ganoderma anticancer component by tower-type gradient percolation - Google Patents
Method for extracting total triterpenoids in lucid ganoderma as lucid ganoderma anticancer component by tower-type gradient percolation Download PDFInfo
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Abstract
The invention provides a method for extracting total triterpenoids in lucid ganoderma as lucid ganoderma anticancer components by a tower-type gradient percolation, which is a percolation tank in a tower structure for forming alcohol concentrations in different gradients to extract lucid ganoderma total triterpenoids in all directions. The method is used for extracting effective components in all directions by adopting edible alcohol with different concentrations, can be carried out at a constant temperature or a low temperature so as not to damage the effective components of medicine, has complete extraction and high content of effective components, and is suitable for extraction of effective components in rare Chinese medicinal materials.
Description
Technical field
The invention belongs to the Chinese medicine extraction field, more specifically relate to tower-type gradient percolation extracting ganoderma anticancer component Ganoderma total triterpenes extracting process.
Background technology
That Ganoderma just is considered to since ancient times is lucky, rich and honour, fine, the life-prolonging symbol, and the title of " Herba mesonae chinensis ", " Ganoderma " is arranged, chinese tradition medical science for a long time a direct-view Ganoderma be strengthening by means of tonics, consolidate set upright, the precious Chinese herbal medicine of life lengthening.365 kinds of Chinese medicines of record in China's the most ancient existing Chinese medicine ancient books and records Shennong's Herbal, be divided into the upper, middle and lower three products, in 120 kinds of top grade Chinese medicines, Ganoderma is arranged and is higher than Radix Ginseng, be medicine-feeding, and Ganoderma is divided into six sesames according to the form and the color of Ganoderma class, think Ganoderma " pacify smart soul, increase wisdom; the beneficial motive; hard muscles and bones, good color, food is made light of one's life by commiting suicide not oldly for a long time, and the angle prolongs life ".Say that Ganoderma is " medicine of going up very much " in " Chinese force in pass ", " and eat it, the day after tomorrow and always ".Put down in writing in the Compendium of Material Medica that the famous medical scholar's Li Shizhen (1518-1593 A.D.) of the Ming Dynasty is write: " Ganoderma bitter in the mouth, flat, nontoxic, the beneficial motive; invigorate blood circulation is gone into heart hyperemia, helps the heart to fill arteries and veins; to calm the nerves, lung benefiting gas, tonifying liver gas; invigorating middle warmer increases wisdom, good color; sharp joint, hard muscles and bones ... " now, Ganoderma was formally recorded by the Pharmacopoeia of the People's Republic of China in 2000 as medicine, it is again the new resource food of health ministry approval simultaneously, has no side effect, can medicine-food two-purpose." U.S.'s medical herbs pharmacopeia and treatment summary (American Herbal Pharmacopoeia and Therapeutic Compendium) " also records Ganoderma.
Ganoderma and effective ingredient thereof have pharmacological action widely, can be used for preventing and treating multiple diseases such as chronic bronchitis, neurasthenia, hyperlipidemia, coronary heart disease, hepatitis, leukopenia, tumor.Its " strengthening the body resistance " effect still can be used for weakness due to chronic disease and health care for the middle and old aged, but health invigorating, slow down aging.
Extracting method for the Ganoderma active ingredient in the prior art is more single, and the extraction of effective ingredient is comprehensive inadequately, and the tower-type gradient percolation can extract Ganoderma anticancer component Ganoderma total triterpenes comprehensively.
Summary of the invention
The present invention is in order to solve in the process of extracting the anticancer component Ganoderma total triterpenes in the Ganoderma, traditional method is more single, can not extract the problem of Ganoderma total triterpenes comprehensively, a kind of extracting process of tower-type gradient percolation extracting ganoderma anticancer component Ganoderma total triterpenes is proposed, thus the problem that the effective ingredient of solution Ganoderma total triterpenes can not extract comprehensively.
Percolation is to add the leaching solvent to make its infiltrate medicated powder in medicated powder, flow out a kind of leaching method of leachate from the bottom, be to utilize tower structure to form the principle of different gradient concentrations, the method of the abundant anticancer composition Ganoderma total triterpenes of extracting ganoderma, used raw material is the mixture of Ganoderma sporophore and Ganoderma spore powder, and its detailed process is:
1) Ganoderma sporophore is got cap more than 8 centimetres, and is dry, do not have the Ganoderma coarse pulverization processing of going mouldy in 2 centimetres of the stems, to the Ganoderma fineness be 20～60 orders;
2) Ganoderma spore powder is crossed 120 mesh sieves through purifying, and control moisture is at 3%-9%, and breaking cellular wall is processed, and sporoderm-broken rate requires 98%, obtains Ganoderma spore powder;
3) 20～60 order Ganoderma sporophore powder and Ganoderma spore powder are mixed, according to the ratio of liquid material than 8:1, add 95% an amount of ethanol and soaked into 2-4 hours, medicinal material coarse powder is fully expanded, gradation is lined with in the percolator of absorbent cotton bottom packing into equably, installs the back at every turn and compresses with wooden stick;
4) adorned Chinese crude drug, gauze on the upper cover, and press the handstone that one deck is cleaned; In order to avoid behind the adding solvent medicated powder is floated.Open the switch of percolation filter end opening then, add solvent from percolator top at leisure again, when closing, liquid closes switch when end opening flows out, pour into effusive liquid in the percolator again, continue to add that solvent is paramount to be gone out till 5-10 centimetre in the medicated powder face, add a cover then, place and open the end opening switch after 48 hours, percolate is slowly flowed out;
5) should constantly replenish Different concentrations of alcohol in the percolation process,, successively decrease by 5% concentration to 85% from 90%, up to, the ethanol of 50% concentration, last water extraction make solvent all the time submergence medicated powder measure in accordance with regulations and collect the liquid of filtering, reclaim solvent, concentrate, spray drying promptly gets the Ganoderma total triterpenes extract.
The advantage of tower-type gradient percolation is to be loaded in the percolator through the medicinal powder after the suitable processing, utilize tower structure to form the leaching solvent strength of different gradients, the leaching solvent of each gradient adds from percolator top, and solvent infiltrate medical material layer down leaches the method for various active ingredients in the flow process.The tower-type gradient percolation is had relatively high expectations to medical material granularity and process conditions, and the triterpenes composition that relatively is suitable for the extraction of rare medicinal herbs, particularly Ganoderma is a lot, needs to adopt the comprehensive extraction effective ingredient of edible ethanol of variable concentrations; Can carry out under room temperature or cryogenic conditions, therefore can not destroy the effective ingredient of medicine, extraction is complete, the effective component content height.
The specific embodiment
Tower-type gradient percolation extracting ganoderma anticancer component Ganoderma total triterpenes extracting process, its detailed process is:
1) Ganoderma sporophore is got cap more than 8 centimetres, and is dry, do not have the Ganoderma coarse pulverization processing of going mouldy in 2 centimetres of the stems, to the Ganoderma fineness be 20～60 orders;
2) Ganoderma spore powder is crossed 120 mesh sieves through purifying, and control moisture is at 3%-9%, and breaking cellular wall is processed, and sporoderm-broken rate requires 98%, obtains Ganoderma spore powder;
3) 20～60 order Ganoderma sporophore powder and Ganoderma spore powder are mixed, according to the ratio of liquid material than 8:1, add 95% an amount of ethanol and soaked into 2-4 hours, medicinal material coarse powder is fully expanded, gradation is lined with in the percolator of absorbent cotton bottom packing into equably, installs the back at every turn and compresses with wooden stick;
4) adorned Chinese crude drug, gauze on the upper cover, and press the handstone that one deck is cleaned; In order to avoid behind the adding solvent medicated powder is floated.Open the switch of percolation filter end opening then, add solvent from percolator top at leisure again, when closing, liquid closes switch when end opening flows out, pour into effusive liquid in the percolator again, continue to add that solvent is paramount to be gone out till 5-10 centimetre in the medicated powder face, add a cover then, place and open the end opening switch after 48 hours, percolate is slowly flowed out;
5) should constantly replenish Different concentrations of alcohol in the percolation process,, successively decrease by 5% concentration to 85% from 90%, up to, the ethanol of 50% concentration, last water extraction make solvent all the time submergence medicated powder measure in accordance with regulations and collect the liquid of filtering, reclaim solvent, concentrate, spray drying promptly gets the Ganoderma total triterpenes extract.
Below be the several embodiments of the present invention, further specify the present invention, but the present invention be not limited only to this according to claim.
Embodiment 1
Tower-type gradient percolation extracting ganoderma anticancer component Ganoderma total triterpenes extracting process, its detailed process is:
1) Ganoderma sporophore is got cap more than 8 centimetres, and is dry, do not have the Ganoderma coarse pulverization processing of going mouldy in 2 centimetres of the stems, to the Ganoderma fineness be 20 orders;
2) Ganoderma spore powder is crossed 120 mesh sieves through purifying, and control moisture is in 3%, and breaking cellular wall is processed, and sporoderm-broken rate requires 98%, obtains Ganoderma spore powder;
3) 90% Ganoderma sporophore powder and 10% Ganoderma spore powder are mixed, according to the ratio of liquid material than 8:1, add 95% an amount of ethanol and soaked into 2 hours, medicinal material coarse powder is fully expanded, gradation is lined with in the percolator of absorbent cotton bottom packing into equably, installs the back at every turn and compresses with wooden stick;
4) adorned Chinese crude drug, gauze on the upper cover, and press the handstone that one deck is cleaned; In order to avoid behind the adding solvent medicated powder is floated.Open the switch of percolation filter end opening then, add solvent from percolator top at leisure again, when closing, liquid closes switch when end opening flows out, pour into effusive liquid in the percolator again,, continue to add that solvent is paramount to be gone out till 5 centimetres in the medicated powder face, add a cover then, place and open the end opening switch after 48 hours, percolate is slowly flowed out;
5) should constantly replenish Different concentrations of alcohol in the percolation process,, successively decrease by 5% concentration to 85% from 90%, up to, the ethanol of 50% concentration, last water extraction make solvent all the time submergence medicated powder measure in accordance with regulations and collect the liquid of filtering, reclaim solvent, concentrate, spray drying promptly gets the Ganoderma total triterpenes extract.
Embodiment 2
1) Ganoderma sporophore is got cap more than 8 centimetres, and is dry, do not have the Ganoderma coarse pulverization processing of going mouldy in 2 centimetres of the stems, to the Ganoderma fineness be 40 orders;
2) Ganoderma spore powder is crossed 120 mesh sieves through purifying, and control moisture is 5%, and breaking cellular wall is processed, and sporoderm-broken rate requires 98%, obtains Ganoderma spore powder;
3) 80% Ganoderma sporophore powder and 20% Ganoderma spore powder are mixed, according to the ratio of liquid material than 8:1, add 95% an amount of ethanol and soaked into 3 hours, medicinal material coarse powder is fully expanded, gradation is lined with in the percolator of absorbent cotton bottom packing into equably, installs the back at every turn and compresses with wooden stick;
4) adorned Chinese crude drug, gauze on the upper cover, and press the handstone that one deck is cleaned; In order to avoid behind the adding solvent medicated powder is floated.Open the switch of percolation filter end opening then, add solvent from percolator top at leisure again, when closing, liquid closes switch when end opening flows out, pour into effusive liquid in the percolator again, continue to add that solvent is paramount to be gone out till 7 centimetres in the medicated powder face, add a cover then, place and open the end opening switch after 48 hours, percolate is slowly flowed out;
5) should constantly replenish Different concentrations of alcohol in the percolation process,, successively decrease by 5% concentration to 85% from 90%, up to, the ethanol of 50% concentration, last water extraction make solvent all the time submergence medicated powder measure in accordance with regulations and collect the liquid of filtering, reclaim solvent, concentrate, spray drying promptly gets the Ganoderma total triterpenes extract.
Embodiment 3
1) Ganoderma sporophore is got cap more than 8 centimetres, and is dry, do not have the Ganoderma coarse pulverization processing of going mouldy in 2 centimetres of the stems, to the Ganoderma fineness be 60 orders;
2) Ganoderma spore powder is crossed 120 mesh sieves through purifying, and control moisture is 9%, and breaking cellular wall is processed, and sporoderm-broken rate requires 98%, obtains Ganoderma spore powder;
3) 50% Ganoderma sporophore powder and 50% Ganoderma spore powder are mixed, according to the ratio of liquid material than 8:1, add 95% an amount of ethanol and soaked into 4 hours, medicinal material coarse powder is fully expanded, gradation is lined with in the percolator of absorbent cotton bottom packing into equably, installs the back at every turn and compresses with wooden stick;
4) adorned Chinese crude drug, gauze on the upper cover, and press the handstone that one deck is cleaned; In order to avoid behind the adding solvent medicated powder is floated.Open the switch of percolation filter end opening then, add solvent from percolator top at leisure again, when closing, liquid closes switch when end opening flows out, pour into effusive liquid in the percolator again, continue to add that solvent is paramount to be gone out till 10 centimetres in the medicated powder face, add a cover then, place and open the end opening switch after 48 hours, percolate is slowly flowed out;
5) should constantly replenish Different concentrations of alcohol in the percolation process,, successively decrease by 5% concentration to 85% from 90%, up to, the ethanol of 50% concentration, last water extraction make solvent all the time submergence medicated powder measure in accordance with regulations and collect the liquid of filtering, reclaim solvent, concentrate, spray drying promptly gets the Ganoderma total triterpenes extract.
Embodiment 4
1) Ganoderma spore powder is crossed 120 mesh sieves through purifying, and control moisture is 9%, and breaking cellular wall is processed, and sporoderm-broken rate requires 98%, obtains 120 order Ganoderma spore powders;
2) Ganoderma spore powder according to the ratio of liquid material than 8:1, adds 95% an amount of ethanol and soaked into 4 hours, and medicinal material coarse powder is fully expanded, and is lined with in the percolator of absorbent cotton bottom gradation is packed into equably, installs the back at every turn and compresses with wooden stick;
3) adorned Chinese crude drug, gauze on the upper cover, and press the handstone that one deck is cleaned; In order to avoid behind the adding solvent medicated powder is floated.Open the switch of percolation filter end opening then, add solvent from percolator top at leisure again, when closing, liquid closes switch when end opening flows out, pour into effusive liquid in the percolator again, continue to add that solvent is paramount to be gone out till 10 centimetres in the medicated powder face, add a cover then, place and open the end opening switch after 48 hours, percolate is slowly flowed out;
4) should constantly replenish Different concentrations of alcohol in the percolation process,, successively decrease by 5% concentration to 85% from 90%, up to, the ethanol of 50% concentration, last water extraction make solvent all the time submergence medicated powder measure in accordance with regulations and collect the liquid of filtering, reclaim solvent, concentrate, spray drying promptly gets the Ganoderma total triterpenes extract.
Claims (6)
1. tower-type gradient percolation extracting ganoderma anticancer component Ganoderma total triterpenes extracting process, it is characterized in that: this is a kind of percolation jar of tower structure, utilizes tower structure to form the concentration of alcohol of different gradients, the total triterpene of comprehensive extracting ganoderma.
2. tower-type gradient percolation extracting ganoderma anticancer component Ganoderma total triterpenes extracting process, it is characterized in that: used Ganoderma is the mixture of Ganoderma sporophore and Ganoderma spore powder.
3. according to the content of claim 2, it is characterized in that: extraction feed is a Ganoderma spore powder.
4. according to the content of claim 2, it is characterized in that: described Ganoderma sporophore is got cap more than 8 centimetres, and is dry, do not have the Ganoderma coarse pulverization processing of going mouldy in 2 centimetres of the stems, to the Ganoderma fineness be 20～60 orders.
5. according to the content of claim 2, it is characterized in that: described Ganoderma spore powder is crossed 120 mesh sieves through purifying, and control moisture is at 3%-9%, and breaking cellular wall is processed, and sporoderm-broken rate requires 98%, obtains Sporoderm-broken Ganoderma Lucidum Spore powder.
6. according to the content of claim 1, it is characterized in that: should constantly replenish Different concentrations of alcohol in the percolation process from 90%, to 85%, successively decrease by 5% concentration, up to, the ethanol of 50% concentration, last water extraction make solvent all the time submergence medicated powder measure in accordance with regulations and collect the liquid of filtering, reclaim solvent, concentrate, spray drying promptly gets the Ganoderma total triterpenes extract.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102430262A (en) * | 2011-09-06 | 2012-05-02 | 毛景华 | Device and method for extracting lucid ganoderma total triterpene |
| CN102614227A (en) * | 2012-04-06 | 2012-08-01 | 毛景华 | Ganoderma pill and processing method thereof |
| CN107334807A (en) * | 2017-07-14 | 2017-11-10 | 武夷山元生泰生物科技有限公司 | A kind of ganoderma lucidum radix tetrastigme pulvis with treatment lung cancer effect and preparation method thereof |
| CN112048418A (en) * | 2020-07-28 | 2020-12-08 | 武夷山元生泰生物科技有限公司 | Lucid ganoderma health-preserving wine and preparation method thereof |
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- 2010-08-12 CN CN2010102516416A patent/CN101904876A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102430262A (en) * | 2011-09-06 | 2012-05-02 | 毛景华 | Device and method for extracting lucid ganoderma total triterpene |
| CN102430262B (en) * | 2011-09-06 | 2013-10-09 | 毛景华 | Device and method for extracting lucid ganoderma total triterpene |
| CN102614227A (en) * | 2012-04-06 | 2012-08-01 | 毛景华 | Ganoderma pill and processing method thereof |
| CN107334807A (en) * | 2017-07-14 | 2017-11-10 | 武夷山元生泰生物科技有限公司 | A kind of ganoderma lucidum radix tetrastigme pulvis with treatment lung cancer effect and preparation method thereof |
| CN112048418A (en) * | 2020-07-28 | 2020-12-08 | 武夷山元生泰生物科技有限公司 | Lucid ganoderma health-preserving wine and preparation method thereof |
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Open date: 20101208 |