CN101880197B - Microbial compound fertilizer and application thereof - Google Patents
Microbial compound fertilizer and application thereof Download PDFInfo
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- CN101880197B CN101880197B CN200910219977A CN200910219977A CN101880197B CN 101880197 B CN101880197 B CN 101880197B CN 200910219977 A CN200910219977 A CN 200910219977A CN 200910219977 A CN200910219977 A CN 200910219977A CN 101880197 B CN101880197 B CN 101880197B
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 75
- 230000000813 microbial effect Effects 0.000 title abstract description 6
- 150000001875 compounds Chemical class 0.000 title abstract 8
- 210000003608 fece Anatomy 0.000 claims abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 16
- 239000010871 livestock manure Substances 0.000 claims abstract description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims abstract description 13
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims abstract description 11
- 241000186361 Actinobacteria <class> Species 0.000 claims abstract description 10
- 241000191043 Rhodobacter sphaeroides Species 0.000 claims abstract description 10
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 8
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000004021 humic acid Substances 0.000 claims abstract description 8
- 239000010802 sludge Substances 0.000 claims abstract description 4
- 230000035800 maturation Effects 0.000 claims abstract 2
- 239000002131 composite material Substances 0.000 claims description 47
- 244000005700 microbiome Species 0.000 claims description 25
- 239000002689 soil Substances 0.000 claims description 25
- 239000000126 substance Substances 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 18
- 229910052757 nitrogen Inorganic materials 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 14
- 229910052700 potassium Inorganic materials 0.000 claims description 13
- 229920000742 Cotton Polymers 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 11
- 235000005822 corn Nutrition 0.000 claims description 11
- 241000589151 Azotobacter Species 0.000 claims description 9
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 9
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 9
- 239000004615 ingredient Substances 0.000 claims description 9
- 239000004571 lime Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 229910052698 phosphorus Inorganic materials 0.000 claims description 9
- 239000010902 straw Substances 0.000 claims description 9
- 239000012747 synergistic agent Substances 0.000 claims description 9
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 7
- 244000068988 Glycine max Species 0.000 claims description 7
- 235000010469 Glycine max Nutrition 0.000 claims description 7
- 229910019142 PO4 Inorganic materials 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- 229910052796 boron Inorganic materials 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 239000011159 matrix material Substances 0.000 claims description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 7
- 239000010452 phosphate Substances 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 6
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 claims description 6
- 239000004202 carbamide Substances 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 239000002426 superphosphate Substances 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 241000282414 Homo sapiens Species 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 241000209140 Triticum Species 0.000 claims description 4
- 235000021307 Triticum Nutrition 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 235000013339 cereals Nutrition 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 235000000346 sugar Nutrition 0.000 claims description 4
- 241000609240 Ambelania acida Species 0.000 claims description 3
- 241000255789 Bombyx mori Species 0.000 claims description 3
- 240000002791 Brassica napus Species 0.000 claims description 3
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 3
- 241000287828 Gallus gallus Species 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 3
- 241001494479 Pecora Species 0.000 claims description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 3
- 239000010905 bagasse Substances 0.000 claims description 3
- ZFXVRMSLJDYJCH-UHFFFAOYSA-N calcium magnesium Chemical compound [Mg].[Ca] ZFXVRMSLJDYJCH-UHFFFAOYSA-N 0.000 claims description 3
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims description 3
- 235000001727 glucose Nutrition 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000010903 husk Substances 0.000 claims description 3
- 235000012204 lemonade/lime carbonate Nutrition 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 229920001223 polyethylene glycol Polymers 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 150000005846 sugar alcohols Chemical class 0.000 claims description 3
- 230000009967 tasteless effect Effects 0.000 claims description 3
- 235000019156 vitamin B Nutrition 0.000 claims description 3
- 239000011720 vitamin B Substances 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 239000002023 wood Substances 0.000 claims description 3
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract description 5
- 235000013399 edible fruits Nutrition 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 239000002932 luster Substances 0.000 abstract description 2
- 241000589152 Azotobacter chroococcum Species 0.000 abstract 2
- 244000025254 Cannabis sativa Species 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 238000012216 screening Methods 0.000 description 8
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 5
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 5
- 240000008067 Cucumis sativus Species 0.000 description 5
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000001816 cooling Methods 0.000 description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 4
- 235000019797 dipotassium phosphate Nutrition 0.000 description 4
- 238000005469 granulation Methods 0.000 description 4
- 230000003179 granulation Effects 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 238000000643 oven drying Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 240000000851 Vaccinium corymbosum Species 0.000 description 2
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 2
- 235000017537 Vaccinium myrtillus Nutrition 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 235000021014 blueberries Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000005056 compaction Methods 0.000 description 2
- 238000013270 controlled release Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 2
- 206010025482 malaise Diseases 0.000 description 2
- 238000011177 media preparation Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 235000000318 Bindesalat Nutrition 0.000 description 1
- 244000106835 Bindesalat Species 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 241000204060 Streptomycetaceae Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 description 1
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940061607 dibasic sodium phosphate Drugs 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 230000013278 single fertilization Effects 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
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- Fertilizers (AREA)
Abstract
The invention relates to an agricultural fertilizer, in particular to a microbial compound fertilizer and application thereof. The microbial compound fertilizer specifically consists of a zymophyte agent and a compound fertilizer, wherein each gram of the compound fertilizer comprises not less than 0.2*108 bacterial cells; the compound fertilizer comprises the following components in percentage by weight: 5 to 15 percent of farmyard manure, 10 to 30 percent of humic acid, 5 to 8 percent of grass carbonic acid, 15 to 30 percent of nutrient and the balance of boric sludge; and the zymophyte agent is obtained by fermenting silicate bacteria, azotobacter chroococcum, actinomycetes, saccharomyces cerevisiae and rhodopseudomonas sphaeroides, wherein the silicate bacteria, the azotobacter chroococcum, the actinomycetes, the saccharomyces cerevisiae and the rhodopseudomonas sphaeroides are mixed together in a weight fraction of 1:1:1:1:1. The microbial compound fertilizer acting on a big field can increase the yield of crops by 20 to 30 percent, and simultaneously can obviously increase the percentage of fertile fruits, reduce fruit dropping and overgrow the fruits; besides, the maturation stage is shortened by 5 to 7 days, the yield-increasing effect is obvious, and simultaneously, the microbial compound fertilizer can obviously improve the appearance, the mouthfeel, the color and luster and the like of products, improve the quality by 1 to 2 grades, and prolong the product freshness date.
Description
Technical field
The present invention relates to agricultural fertilizer, a kind of specifically micro organism composite fertilizer and application thereof.
Background technology
Along with Increase of population, for satisfying the grain needs, the consumption of China's chemical fertilizer is the trend of sustainable growth; Use chemical fertilizer and make a great contribution, but also brought serious environmental to pollute and ecological balance damage owing to use chemical fertilizer for a long time in a large number simultaneously, also cause soil compaction solving the food shortage problem; Make that useful microbe sharply reduces in the soil, the perviousness of soil, oxygen delivery capacity and keep the nutrient balance ability and reduce, a large amount of residues of harmful substances and harmful microorganism; Controlled root system of plant to nutrient, absorption of water; Soil structure is changed, cause that plant growth is bad, quality descends, various diseases are on the rise; The increase of inside plants pesticide residue is seriously threatening human beings'health and safety simultaneously.
Development along with green agriculture; The application in agriculture prodn of bio-fertilizer or composite bio-fertilizer more and more receives people's attention; Generally make base manure and append fertilizer when present most countries is used composite fertilizer and use, both can not take into account, in the main raw material nitrogen phosphorus potassium three big key elements; Nitrogen lacks slow release/controlled release technology, also just loses the stable balance merit between nitrogen and the phosphorus potassium.The nitrogen effect phase is short, and loss amount is big in the use, have to impose nitrogenous fertilizer in plant growth mid-term, and is when topdressing operational cost, effort, efficient with the modern times, the agriculture prodn that simplifies the operation is unbecoming.
Summary of the invention
The object of the invention is to provide a kind of micro organism composite fertilizer and application thereof of single fertilization.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is:
A kind of micro organism composite fertilizer: form by fermenting agent and composite fertilizer, contain in its every gram composite fertilizer and be not less than 0.2 * 10
8Individual mycetocyte, said composite fertilizer by weight percentage, farm manure 5-15%, humic acid 10-30%, careless carbonic acid 5-8%, nutrient substance 15-30%, synergistic agent 5-10%, surplus is a boron mud; Said fermenting agent is that silicate bacteria, blown-ball Azotobacter, actinomycetes, S. cervisiae and Rhodopseudomonas spheroides fermentation form, and silicate bacteria, blown-ball Azotobacter, actinomycetes, S. cervisiae and Rhodopseudomonas spheroides were according to 1: 1: 1: 1: 1 weight fraction mixed.
Said farm manure is for being made up of major ingredient and auxiliary material by ordinary method production, content meter by weight, and wherein major ingredient accounts for 94-97%, and auxiliary material accounts for 3-6%; Major ingredient is by wheat straw, straw, corn cob, cotton seed hull, corn stalk powder, soya bean stem meal, waste cotton, wheat bran, rice husk, wood chip, cotton straw powder, weeds; The alditol slag, the 2-4 kind mixture in xylose residue, bagasse, fresh grain stillage, rapeseed cake, cow dung, chicken manure, horsehit, sheep excrement, rabbit excrement, human excrement, pigsty fertilizer and the pond sludge soil is formed; Auxiliary material is by soybean cake powder; Cotton seed cake, wheat bran, urea, composite fertilizer, superphosphate of lime, fused(calcium magnesium)phosphate, lime powder, terra alba, lime carbonate, plant ash, potassium primary phosphate, sucrose, brown sugar, ammoniacal liquor, the three-dimensional nutrient substance of edible mushrooms, dried yeast tablets, dried silkworm chrysalis meal, glucose and vitamins B
1In 3-6 kind mixture form.
Said nutrient substance is that N, P, K form, wherein 20-30%N, 15-30%P, 10-15%K by weight percentage, and the providing of said P, N, K element, P is P
2O
5Or superphosphate of lime; N is a granular urea; K is Kcl or K
2SO
4
Said synergistic agent is an agricultural synergist.
Said fermenting agent is inoculated silicate bacteria, blown-ball Azotobacter, actinomycetes, S. cervisiae and Rhodopseudomonas spheroides respectively; Then by 1: 1: 1: 1: 1 weight fraction mixed is changeed 60-70 ℃/200,1: 0.5 air flow of dissolved oxygen is cultivated, and is tasteless until culture.
The application of micro organism composite fertilizer: the disposable amount by 25-40 kilogram/mu of said micro organism composite fertilizer is manured into soil in the matrix, then plants farm crop, ripe until farm crop.
The disposable amount by 15-50 kilogram/mu of said micro organism composite fertilizer is manured into soil in the matrix, then plants flowers, until blooming.
The advantage that the present invention had:
1. improve output, improve quality, micro organism composite fertilizer of the present invention is acted on big Tanaka, can make crop yield 20-30%; Can significantly increase percentage of fertile fruit simultaneously, reduce shedding, make fruit loose; In generally 5-7 days in advance ripening stage, effect of increasing production is obvious, possibly obviously improve product appearance, mouthfeel, color and luster etc. simultaneously; Quality improves 1-2 grade, prolongs the product freshness date.
2. the raising chemical fertilizer utilization ratio reduces applying quantity of chemical fertilizer, after the mikrobe in the fertilizer of the present invention gets into soil, and the nitrogen in a large amount of breedings ability fixed airs of active bio bacterial strain; Decompose organic residual body and unavailable phosphorus of crop and potassium in the soil, transform the available nutrient of crop, can reduce applying quantity of chemical fertilizer more than 50%, improve fertilizer utilization ratio greatly, cost is reduced.
3. improve the soil; A large amount of breedings of microbial strains in the fertilizer of the present invention improve the microecosystem of soil greatly, promote the formation of soil aggregate; Strengthen the ventilation property of soil; Improve the preserve moisture and fertility ability of soil, eliminate applying the soil compaction that chemical fertilizer causes throughout the year in a large number, improve soil activation.
Embodiment
Embodiment 1
The cultivation of silicate bacteria (Silicate bacteria): culture medium preparation: sucrose 10g, Nacl10g, potassium hydrogenphosphate 2g, sal epsom 0.5g, ammonium sulfate 1g, CaCO
33g is soluble in water, and PH=7.2 goes into 121 ℃ of triangular flasks, sterilization in 30 minutes, the inoculation of cooling back, and last shaking table 28 ℃/200 changes to be cultivated 48 hours, for use.
The cultivation of blown-ball Azotobacter (Azotobacter chrooccum Beijerinck): the preparation of substratum: potassium primary phosphate 1g, CaCO
32g, iron trichloride 0.1g, sal epsom 0.2g, Nacl 0.2g, Sodium orthomolybdate 0.005g, sucrose 10g are water-soluble, PH7.0, and being inoculated in shaking table 30 ℃/200 after sterilization, the cooling changes and cultivated 48 hours, for use.
Actinomycetes (Actinomycete) are adopted streptomycete (Streptomycetaceae) culture medium preparation; Semen Maydis powder 60g is heated into pasty state with less water dissolving, will add in the culture medium solution of sal epsom 0.2g, potassium hydrogenphosphate 0.25g, Sodium phosphate, dibasic 1g PH=7.4 in triangular flask 121 ℃ again; Sterilization in 30 minutes; The inoculation of cooling back, and 28 ℃/90-200 of shaking table commentaries on classics cultivation 72 hours, for use.
The preparation of S. cervisiae (Saccharomyces cerevisiae) substratum: with boiling 30 minutes in the 100g soybean sprout adding 1000ml water; Get 100ml bean sprouts juice and add 4g yeast extract paste, 15g sucrose 2gNacl, 1g peptone; PH=6.5-6.7 sterilizes 121 ℃, cooling back inoculation in 30 minutes; And shaking table 28 ℃/200 commentaries on classics cultivations 16-20 hour, for use.
The preparation of Rhodopseudomonas spheroides (Rps.globiformis) substratum: 1g ammonium sulfate, 2gNacl, 0.5g potassium hydrogenphosphate, 0.2g sal epsom, 0.05g iron trichloride, 0.05mg copper sulfate, 0.05mg sal epsom, 1mg zinc sulfate, 2g peptone, 0.1g yeast extract paste; PH=7.5 soluble in water; Sterilize 115 ℃; Be cooled to 30 ℃ of inoculations in 15 minutes, illumination 30-35 ℃, 72-120 hour to scarlet bacterium liquid.All for use with last bacteria culture fluid with the nutrient solution of 1000ml.
Fermenting agent is with Semen Maydis powder 10 ‰, sucrose 15 ‰, potassium hydrogenphosphate 0.02 ‰, CaCO
30.3 ‰, sal epsom 0.02 ‰ is processed fermention medium; PH=7.4 before the sterilization then moves into fermentor tank and dissolves 121 ℃ surely; Sterilization in 30 minutes; To be cooled to 30 ℃, then in 1: 1: 1: 1: 1 weight fraction ratio is blended in above-mentioned bacterial classification 60 ℃/200 changes silicate bacteria, blown-ball Azotobacter, actinomycetes, S. cervisiae and Rhodopseudomonas spheroides, 1: 0.5 air flow of dissolved oxygen is cultivated, tasteless until culture.
Micro organism composite fertilizer is made up of fermenting agent and composite fertilizer; With composite fertilizer by weight percentage; Farm manure 5%, humic acid 10%, careless carbonic acid 5%, nutrient substance 15%, synergistic agent 5%, surplus for boron mud mix through screening, oven dry, broken, then add fermenting agent and mix to contain in the every gram composite fertilizer and be not less than 0.2 * 10
8Individual mycetocyte; Mix back granulation, oven drying at low temperature, screening, promptly get micro organism composite fertilizer; Wherein nutrient substance is N, P, K, and said N, P, K element are provided by granular urea, superphosphate of lime, Kcl respectively, and said NPK is 33 content.Said synergistic agent is controlled release long-acting synergistic agent (available from Shenyang Zhongke Green Field Agriculture Development Co., Ltd.).
Said farm manure is for being made up of major ingredient and auxiliary material by ordinary method production, content meter by weight, and wherein major ingredient accounts for 94-97%, and auxiliary material accounts for 3-6%; Major ingredient is by wheat straw, straw, corn cob, cotton seed hull, corn stalk powder, soya bean stem meal, waste cotton, wheat bran, rice husk, wood chip, cotton straw powder, weeds; The alditol slag, the 2-4 kind mixture in xylose residue, bagasse, fresh grain stillage, rapeseed cake, cow dung, chicken manure, horsehit, sheep excrement, rabbit excrement, human excrement, pigsty fertilizer and the pond sludge soil is formed; Auxiliary material is by soybean cake powder; Cotton seed cake, wheat bran, urea, composite fertilizer, superphosphate of lime, fused(calcium magnesium)phosphate, lime powder, terra alba, lime carbonate, plant ash, potassium primary phosphate, sucrose, brown sugar, ammoniacal liquor, the three-dimensional nutrient substance of edible mushrooms, dried yeast tablets, dried silkworm chrysalis meal, glucose and vitamins B
1In 3-6 kind mixture form.
Embodiment 2
Micro organism composite fertilizer is made up of fermenting agent and composite fertilizer; With composite fertilizer by weight percentage; Farm manure 15%, humic acid 30%, careless carbonic acid 8%, nutrient substance 30%, synergistic agent 3%, surplus for boron mud mix through screening, oven dry, broken, then add fermenting agent and mix to contain in the every gram composite fertilizer and be not less than 0.2 * 10
8Individual mycetocyte; Mix back granulation, oven drying at low temperature, screening, promptly get micro organism composite fertilizer.
Embodiment 3
Micro organism composite fertilizer is made up of fermenting agent and composite fertilizer; With composite fertilizer by weight percentage; Farm manure 10%, humic acid 20%, careless carbonic acid 6%, nutrient substance 20%, synergistic agent 4%, surplus for boron mud mix through screening, oven dry, broken, then add fermenting agent and mix to contain in the every gram composite fertilizer and be not less than 0.2 * 10
8Individual mycetocyte; Mix back granulation, oven drying at low temperature, screening, promptly get micro organism composite fertilizer.
Embodiment 4
Micro organism composite fertilizer is made up of fermenting agent and composite fertilizer; With composite fertilizer by weight percentage; Farm manure 9%, humic acid 2 5%, careless carbonic acid 7%, nutrient substance 25%, surplus for boron mud mix through screening, oven dry, broken, then add fermenting agent and mix to contain in the every gram composite fertilizer and be not less than 0.2 * 10
8Individual mycetocyte; Mix back granulation, oven drying at low temperature, screening, promptly get micro organism composite fertilizer.
Application examples
Experimental field soil is that brown moisture soil, quality are husky earth; It is on the lower side medium to require great effort; The soil organism 1.58%, full nitrogen 0.079%, quick-acting nitrogen 74.03ppm, rapid available phosphorus 98.47ppm, available potassium 76.68ppm, PH7.8, experimental cultivar cucumber, green pepper, corn, flowers.
Experiment is one-factor experiment:
A: the disposable amount by 40 kilograms/mu of the obtained micro organism composite fertilizer of the foregoing description is applied the farm crop soil matrix, apply the flowers soil matrix by 25 kilograms/mu amounts.
B: common fertilizer applies matrix and is then irregularly appending fertilising, and cucumber, green pepper chase after nitrogen 10kg/ mu, corn 25kg/ mu, flowers (blueberry) 20kg/ mu.
C: blank.
Every processing triplicate, district's group is arranged at random, 14.23 mu of cucumber, green pepper sub-district areas, 66.7 mu of corn sub-district areas produce by the sub-district is real during 65 mu of results of flowers sub-district area, and cucumber, green pepper are gathered repeatedly.
Interpretation of result
Table 1 cucumber yield
Table 2 green pepper
Table 3 corn
Table 4 flowers (blueberry)
Other applies embodiment gained micro organism composite fertilizer, can make increasing production of rice 16%, wheat increase yield 18%, apple raising the output 17%; Increasing yield of water melons 19%, said product sugar degree increases 0.7-0.9%, and sickness rate reduces to more than 50%; Romaine lettuce raising the output 30%, tomato production promoting 30-40%, eggplant 20-30%; Peanut yield increasing 20%, sweet potato raising the output 30%, Chinese cabbage is increased production 30% above-mentioned crop sickness rate and reduces 50%.
Be example with the corn simultaneously, measure the soil of maize planting, can know that applying the fertile phase of common fertilizer is 70-80 days, applying the fertile phase of fertilizer of the present invention is 120-130 days.
Claims (4)
1. micro organism composite fertilizer is characterized in that: be made up of fermenting agent and composite fertilizer, contain in its every gram composite fertilizer and be not less than 0.2 * 10
8Individual mycetocyte, said composite fertilizer by weight percentage, farm manure 5-15%, humic acid 10-30%, careless carbonic acid 5-8%, nutrient substance 15-30%, surplus is a boron mud; Said fermenting agent is that silicate bacteria, blown-ball Azotobacter, actinomycetes, S. cervisiae and Rhodopseudomonas spheroides fermentation form, and said silicate bacteria, blown-ball Azotobacter, actinomycetes, S. cervisiae and Rhodopseudomonas spheroides were according to 1: 1: 1: 1: 1 weight fraction mixed;
Said nutrient substance is that N, P, K form, wherein 20-30%N, 15-30%P, 10-15%K by weight percentage, and said P is P
2O
5Or superphosphate of lime; N is a urea; K is KCl or K
2SO
4
Silicate bacteria, blown-ball Azotobacter, actinomycetes, S. cervisiae and Rhodopseudomonas spheroides are inoculated respectively; Then by 1: 1: 1: 1: 1 weight fraction mixed 60-70 ℃/200 commentaries on classics, air flow of 1: 0.5 of dissolved oxygen amount in fermention medium are cultivated, and be tasteless until culture;
Said farm manure is for being made up of major ingredient and auxiliary material by ordinary method production, content meter by weight, and wherein major ingredient accounts for 94-97%, and auxiliary material accounts for 3-6%; Major ingredient is by wheat straw, straw, corn cob, cotton seed hull, corn stalk powder, soya bean stem meal, waste cotton, wheat bran, rice husk, wood chip, cotton straw powder, weeds; The alditol slag, the 2-4 kind mixture in xylose residue, bagasse, fresh grain stillage, rapeseed cake, cow dung, chicken manure, horsehit, sheep excrement, rabbit excrement, human excrement, pigsty fertilizer and the pond sludge soil is formed; Auxiliary material is by soybean cake powder, cotton seed cake, wheat bran, urea, superphosphate of lime, fused(calcium magnesium)phosphate, lime powder, terra alba, lime carbonate, plant ash, potassium primary phosphate, sucrose, brown sugar, ammoniacal liquor, dried yeast tablets, dried silkworm chrysalis meal, glucose and vitamins B
1In 3-6 kind mixture form.
2. by the described micro organism composite fertilizer of claim 1, it is characterized in that: said composite fertilizer by weight percentage, farm manure 5-15%, humic acid 10-30%, careless carbonic acid 5-8%, nutrient substance 15-30%, synergistic agent 3-6%, surplus is a boron mud; Said synergistic agent is an agricultural synergist.
3. application by the described micro organism composite fertilizer of claim 1 is characterized in that: the disposable amount by 25-40 kilogram/mu of said micro organism composite fertilizer is manured into soil in the matrix, then plants farm crop, until the farm crop maturation.
4. application by the described micro organism composite fertilizer of claim 1 is characterized in that: the disposable amount by 15-50 kilogram/mu of said micro organism composite fertilizer is manured into soil in the matrix, then plants flowers, until blooming.
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