CN101805695B - Oblique rotational gravity mixed biological reaction equipment - Google Patents

Oblique rotational gravity mixed biological reaction equipment Download PDF

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Publication number
CN101805695B
CN101805695B CN 201010102619 CN201010102619A CN101805695B CN 101805695 B CN101805695 B CN 101805695B CN 201010102619 CN201010102619 CN 201010102619 CN 201010102619 A CN201010102619 A CN 201010102619A CN 101805695 B CN101805695 B CN 101805695B
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Prior art keywords
culture vessel
stationary installation
biological reaction
column
reaction equipment
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CN 201010102619
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Chinese (zh)
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CN101805695A (en
Inventor
杨国松
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Bohui Biopharmaceutical Co.,Ltd.
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FORWELL BIOPHARMACEUTICALS Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C12M27/12—Roller bottles; Roller tubes

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to oblique rotational gravity mixed biological reaction equipment, in particular to biological reaction equipment capable of enabling a container with a columnar main body to perform micro-carrier cell culture. In a frame which plays a role in fixing and supporting, a plurality of columnar culture vessel fixing devices which are parallel to one another and form angles of between 5 and 8 degrees with a horizontal plane are driven by an electric motor to rotate; a plurality of groups of fixing hole sites are symmetrically distributed around rotating shafts on the columnar culture vessel fixing devices and relatively to the centers of the rotating shafts; each group of hole sites consists of at least two fixing holes with centers on the same straight line and parallel planes and a supporting pedestal which has a supporting effect at the bottom; the lines or rows of columnar culture vessel fixing devices, the lines or rows of columnar culture vessel fixing devices and the electric motor can be driven by a transmission belt or a gear; the reaction equipment can comprise a control circuit part; and the reaction equipment can be accommodated in a small-sized temperature control vessel or arranged in a temperature control chamber.

Description

Oblique rotational gravity mixed biological reaction equipment
Technical field
The microcarrier cell cultures is the main method that mass cell is cultivated, and the conventional bio-reactor that is used for the microcarrier cell cultures costs an arm and a leg, and complex structure needs the operator of specialty to use.
The biological reaction apparatus that the present invention relates to is economical and practical, and is simple to operate, makes the microcarrier cell cultures equally easy as common cell bottle cell cultures.
Background technology
Because traditional rolling bottle culturing cell exists labor capacity big, required bottle quantity is many, and contaminating microorganisms consumes energy greatly easily, produces shortcomings such as dirt is many, and the microcarrier cell culture technology receives publicity day by day.And the bio-reactor of import costs an arm and a leg, and the home-made bio-reactor is cheap, the bad again assurance of culture effect and stability.
Summary of the invention
In order to address the above problem, the invention provides a kind of microcarrier biological reaction apparatus can be that column culture vessel (like 3L rolling bottle etc.) carries out the microcarrier cell cultures through main body, reaches high-cell density, economical and practical.
The technical scheme that the present invention adopted is: a kind of main body that makes is the biological reaction apparatus that the container of column carries out the microcarrier cell cultures; In the framework that plays fixing and supporting role; Rotate by direct motor drive 1 row or multirow, 1 row or multiple row column culture vessel stationary installation, carry out the microcarrier cell cultures.
Rotate about it axle on its column culture vessel stationary installation and relatively rotate a revenue centre and be symmetrically distributed with 2 groups, 3 groups or organize fixed hole position more, position, every group of hole by at least 2 centers on same straight line and plane, place fixed orifices parallel to each other and the base for supporting that plays the support effect in the bottom form.
Its each column culture vessel stationary installation parallel to each other and with horizontal plane be 5～80 degree angles.
Between its each row or each colonnade shape culture vessel stationary installation, can use transmission belt transmission or geartransmission between each row or each colonnade shape culture vessel stationary installation and the phonomoter.
It can contain the pilot circuit part.
It can be contained in the small-sized temperature control container, also can it directly be put in the temperature-controlled chamber.
Description of drawings
Fig. 1, Fig. 2 are the side-view of realizing two kinds of types of the design's thinking
Fig. 3 is the frontview of the column culture vessel stationary installation of horizontal positioned
Fig. 4 is the schematic perspective view with the column culture vessel stationary installation at horizontal plane journey 5～80 degree angles
Fig. 5 is the framework of its fixed support effect of frontview (1) of multiple lines and multiple rows and column culture vessel stationary installations horizontal plane journey 5～80 degree angles; (2) phonomoter; (3) column culture vessel stationary installation; (4) crossbeam; (5) belt or the gear of connection column culture vessel stationary installation; (6) belt or the gear of connection column culture vessel stationary installation and phonomoter; (7) fixed hole position of placement column culture vessel; (8) column culture vessel stationary installation rotation axis; (9) fixed orifices of placement column culture vessel; (10) base for supporting of placement column culture vessel;
Embodiment
For making the feature and advantage of the present invention can be more obviously understandable, the present invention be done further detailed explanation below in conjunction with accompanying drawing and embodiment.
Fig. 1, Fig. 2 are the side-view of realizing two kinds of types of the design's thinking; Connecting on the crossbeam (4) in framework (1) by 1 row or multirow, 1 row or multiple row is parallel to each other and with the column culture vessel stationary installation (3) at horizontal plane journey 5～80 degree angles; Every row or every capable column culture vessel stationary installation (3) are connected by belt or gear (5); And be connected to common phonomoter (2) through belt or gear (6), rotate by pilot circuit control direct motor drive.
Fig. 3 is the frontview of the column culture vessel stationary installation of horizontal positioned, the fixed hole position (7) that 2 groups, 3 groups or many groups are placed the column culture vessels round its revenue centre symmetric offset spread of rotation axis (8).Fig. 4 is the schematic perspective view with the column culture vessel stationary installation at horizontal plane journey 5～80 degree angles, and each is placed on the fixed hole position of column culture vessel at least 2 and places the fixed orifices (9) of column culture vessels and the base for supporting (10) of 1 placement column culture vessel.
Fig. 5 is frontviews multiple lines and multiple rows and column culture vessel stationary installations horizontal plane journey 5～80 degree angles.

Claims (4)

  1. One kind can to make main body be the biological reaction apparatus that the culture vessel of column carries out the microcarrier cell cultures; It is characterized in that in the framework of fixing and supporting role; By the direct motor drive multirow; Multiple row column culture vessel stationary installation is rotated, and carries out the microcarrier cell cultures, and each column culture vessel stationary installation is parallel to each other and all tilt to be connected in the support frame; On the column culture vessel stationary installation of its inclination, rotate about it axle and relatively rotate a revenue centre and be symmetrically distributed with many group fixed hole positions, every group of fixed hole position by at least two centers on same straight line and plane, place fixed orifices parallel to each other and the base for supporting that plays the support effect in the bottom form; Its each column culture vessel stationary installation be parallel to each other and with horizontal plane be 5～80 degree angles; Between the column culture vessel stationary installation that its each row, each row tilt, use the transmission belt transmission between column culture vessel stationary installation that each row, each row tilt and the phonomoter.
  2. 2. according to claim 1 a described biological reaction apparatus, it is characterized in that it contains the pilot circuit part, pilot circuit comprises power switch, electromotor velocity regulating circuit, corresponding button.
  3. 3. according to any described biological reaction apparatus in the claim 1～2, it is characterized in that it is placed in the temperature control container.
  4. 4. according to claim 3 a described biological reaction apparatus, it is characterized in that described temperature control container comprises greenhouse, incubator.
CN 201010102619 2010-01-29 2010-01-29 Oblique rotational gravity mixed biological reaction equipment Active CN101805695B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201010102619 CN101805695B (en) 2010-01-29 2010-01-29 Oblique rotational gravity mixed biological reaction equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201010102619 CN101805695B (en) 2010-01-29 2010-01-29 Oblique rotational gravity mixed biological reaction equipment

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CN101805695A CN101805695A (en) 2010-08-18
CN101805695B true CN101805695B (en) 2012-06-13

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104521946B (en) * 2015-01-26 2016-08-17 深圳康泰生物制品股份有限公司 Cell freezing apparatus and method
DK3430119T3 (en) * 2016-03-14 2023-09-18 Omnibrx Biotechnologies Private Ltd A BIOREACTOR SYSTEM AND ITS OPERATION

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1546642A (en) * 2003-12-10 2004-11-17 上海伯瑞生物技术发展有限公司 Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture
CN1321175C (en) * 2004-01-13 2007-06-13 中国科学院动物研究所 Microcarrier for large-scale culture cell
CN200949106Y (en) * 2006-09-22 2007-09-19 韩毅鹏 Tilted two-level rotating cylinder bioreactor
CN100360657C (en) * 2005-03-30 2008-01-09 韩毅鹏 Horizontal drum type bioreactor
CN201753358U (en) * 2010-01-29 2011-03-02 福尔生物制药股份有限公司 Oblique and rotary gravity mixed biological reaction unit

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1546642A (en) * 2003-12-10 2004-11-17 上海伯瑞生物技术发展有限公司 Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture
CN1321175C (en) * 2004-01-13 2007-06-13 中国科学院动物研究所 Microcarrier for large-scale culture cell
CN100360657C (en) * 2005-03-30 2008-01-09 韩毅鹏 Horizontal drum type bioreactor
CN200949106Y (en) * 2006-09-22 2007-09-19 韩毅鹏 Tilted two-level rotating cylinder bioreactor
CN201753358U (en) * 2010-01-29 2011-03-02 福尔生物制药股份有限公司 Oblique and rotary gravity mixed biological reaction unit

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Owner name: ZHONGKE BIOLOGICAL PHARMACEUTICAL CO., LTD.

Free format text: FORMER NAME: FORWELL BIOPHARMACEUTICALS CO., LTD.

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Address after: 065201 No. 1155 Development Zone, Sanhe, Hebei, Yanjiao

Patentee after: ZHONGKE BIOPHARM CO.,LTD.

Address before: 065201 Yingbin Road 38-2, Yanjiao Development Zone, Hebei, Sanhe

Patentee before: FORWELL BIOPHARMACEUTICALS Co.,Ltd.

CP01 Change in the name or title of a patent holder
CP01 Change in the name or title of a patent holder

Address after: No. 1155, Yanjiao Development Zone, Sanhe City, Hebei Province, 065201

Patentee after: Bohui Biopharmaceutical Co.,Ltd.

Address before: No. 1155, Yanjiao Development Zone, Sanhe City, Hebei Province, 065201

Patentee before: ZHONGKE BIOPHARM CO.,LTD.