CN101796922A

CN101796922A - Betula alnoides leaf bud tissue culture rapid propagation technique

Info

Publication number: CN101796922A
Application number: CN200910113863A
Authority: CN
Inventors: 谌红辉
Original assignee: Experimental Center Of Tropical Forestry Chinese Academy Of Forestry
Current assignee: Experimental Center Of Tropical Forestry Chinese Academy Of Forestry
Priority date: 2009-02-11
Filing date: 2009-02-11
Publication date: 2010-08-11

Abstract

The invention discloses a betula alnoides leaf bud tissue culture rapid propagation technique, which is a seed breeding technique for enlarging propagation of excellent units by a neuter method and a biological technique developed according to totipotency of plant cells. Through selection of excellent betula alnoides, inducing differentiation of excellent leaf bud, proliferation and rooting culture and tree stock transplanting, high-yield and efficient excellent sprouts can be cultured rapidly and the wood single yield of the forest land is increased.

Description

Betula alnoides leaf bud tissue culture rapid propagation technique

Affiliated technical field

Patent of the present invention belongs to the forestry biotechnology, is mainly used in the quick breeding of Birch choiceness nursery stock.

Background technology

Plant Tissue Breeding is the biotechnology that the totipotency according to plant cell grows up, and for Forest Tree Genetic Breeding provides a cover advanced breeding method, its further application can be brought into play the effect of Forest Tree Genetic Breeding in production of forestry better.At present, the Birch nursery stock that China cultivates mainly is a seedling, and seedling is difficult to reach colony's aim of high yield because the diversity of genetic material afforestation back growth phenotype differs.Clone nursery stock is identical because of gene, afforestation back phenotype homogeneous.Adopt the Birch tissue culture rapid propagation technique can breed a large amount of choiceness nursery stocks, thereby can reach colony's aim of high yield.

Summary of the invention

At present, forest department builds the used nursery stock of Birch timber plantation and is the seedling that cultivating seeds forms, but behind the seedling forestation because the inhomogeneity of genetic material, the tree growth quality is uneven, is difficult to improve the forest land timber yield.This technological invention utilizes the vegetative propagation technique of Plant Tissue Breeding, and Birch select tree leaf bud is carried out asexual propagation, and the choiceness nursery stock of cultivating neat high yield is used for producing afforestation, can significantly improve the forest land timber yield.

This invention solves the technical scheme that the betula alnoides leaf bud tissue culture rapid propagation technique problem is adopted

1. the good elite stand of Birch seeds that has reached forest germplasm appraisal age is selected and estimated.

2. the young tender leaf bud explant to the good elite stand selected carries out the clip collection with scissors, takes back the laboratory and handles, and uses alcohol (concentration 75%) and mercury solution (concentration 3 ‰) disinfection then.

3. solve different minimal medium compositions, different hormone kind and concentration and leaf bud explantation tissue is induced the influence of differentiation, solve the influence of different medium, hormone kind and concentration, sum up the supporting medium allocation plan of betula alnoides leaf bud tissue culture tissue culture seeding stem segment sprouting of lateral bud and hestening rooting.

4. after the tissue-culture container seedling that will transplant being carried out hardening, carry out transplanting breeding, the solution group is trained the seedling replanting and the cultivation technology of the seedling of taking root.

The beneficial effect of this invention is embodied in following 4 aspects

1. the novelty of seedling and protection.Good new varieties are the behave of a property changed with efficient clone nursery stock afforestation for the traditional commodity seedling forestation in south, and it is an effective remedial measure that factorial seedling growth produces in great shortage for good seed.

2. locality of seeds and high efficiency.Birch is that the precious native soil speed in south is given birth to the broad-leaved commerical tree species, and is widely distributed, and good new varieties and the neat homogeneous of choiceness nursery stock afforestation back growth can increase substantially the forest land productivity, have good ecology, economy and society benefit.

3. strategic.The timber dependence on import is difficult to be for a long time, can improve the self-produced ability of timber behind this project success in a short time rapidly, and can change south pine, China fir, situation that the eucalyptus reproducting tree species are single, for the industrial timberland construction of base of southwest and South China provides choice of tree species efficiently.

Description of drawings:

Fig. 1 Birch select tree is selected.

Fig. 2 Birch select tree leaf bud is selected.

Fig. 3 betula alnoides leaf bud is disinfected.

Fig. 4 betula alnoides leaf bud is induced differentiation.

Fig. 5 induces stem section sprouting of lateral bud and takes root.

The culture of rootage of Fig. 6 tissue cultivating seedling.

Fig. 7 Birch tissue cultivating seedling is transplanted.

Fig. 8 Birch tissue cultivating seedling afforestation upgrowth situation after 2 years.

Embodiment

In Fig. 1, the good elite stand of Birch that has reached forest germplasm appraisal age is selected and estimates.

In Fig. 2, the young tender leaf bud explant of the good elite stand selected is carried out the clip collection with scissors.

In Fig. 3, the leaf bud of gathering is taken back the laboratory handle, use alcohol (concentration 75%) and mercury solution (concentration 3 ‰) disinfection then.

In Fig. 4, solve different minimal medium compositions, different hormone kind and concentration are induced differentiation to leaf bud explantation tissue influence.

In Fig. 5, solve different medium, hormone kind and concentration the tissue culture seeding stem segment sprouting of lateral bud is also taken root simultaneously, reach the effect of propagation.

In Fig. 6, solve the influence that different medium, hormone kind and concentration are taken root to tissue cultivating seedling, sum up the supporting medium allocation plan of Birch tissue culture.

In Fig. 7, the tissue-culture container seedling that transplant carried out hardening after, carry out transplanting breeding, the training of solution group the take root seedling replanting and the cultivation technology of seedling.

In Fig. 8, Birch tissue cultivating seedling afforestation upgrowth situation after 2 years.

Claims

1. the quick propagating technology of betula alnoides leaf bud tissue culture, adopt leaf bud to do explant material, utilize supporting tissue culture medium (TCM) allocation plan, also take root simultaneously through sprouting of lateral bud propagation, reach the effect of quick breeding, it is characterized in that: after the differentiation of leaf bud explant induction, adopt to promote stem section sprouting of lateral bud also to take root simultaneously, reach the effect of quick breeding.

2. the quick propagating technology of betula alnoides leaf bud tissue culture according to claim 1, it is characterized in that: adopt leaf bud to do explant after medium is induced differentiation, adopt the sprouting of lateral bud elongation growth of supporting medium promotion stem section again and promote base portion to take root, circulation clip stem section is cultivated, and reaches the effect of quick breeding.