CN101526532A

CN101526532A - Narcotic saliva detection

Info

Publication number: CN101526532A
Application number: CN200810302091A
Authority: CN
Inventors: 陈桂勇
Original assignee: Individual
Current assignee: Individual
Priority date: 2008-06-10
Filing date: 2008-06-10
Publication date: 2009-09-09

Abstract

The invention relates to narcotic saliva detection belonging to the field of biological medicines, biological separation, biological devices, reagents and relative detection reagents. A colloidal gold immunity tomography technology is very developed as a development platform technology for the product, but the development key of the product lies in the high sensitivity and the high specificity of a monoclonal antibody and the optimization of the product manufacture technological process. In order to evade the technological problems, the monoclonal antibody used for product development can be firstly separated so that monoclonal antibody stubs with the optimal sensitivity and the optimal specificity can be selected for protein labeling and can meet the special requirements for the saliva detection.

Description

Narcotic saliva detects

Affiliated technical field:

Biological medicine. bio-separation, device, reagent and coherent detection reagent.

Background technology:

The drugs quick detection reagent that is adopted on the domestic and international market all is to be that sample detects with the urine at present; The present greatest problem that faces of these series of products is exactly the sample dirt, defective such as mingle/substitute easily.And in the world cry day high property privacy and human rights issue.

Summary of the invention:

For overcome sample dirt that present drugs detect, mingle easily/substitute, defective such as the property invaded privacy. saliva detection will be clinical disease diagnosis. the forbidden drug of family health care self check and forensic science or drugs detect. open up a brand-new field.Its sampling method is simple, and Noninvasive, and sample totally and not has infectiousness, also can save great amount of manpower and material resources, and drug concentration and blood concentration in the saliva are closely related; This has just been avoided the infectiousness danger of blood testing and the sample dirt of urine detection, mingle easily/substitute, defectives such as the property invaded privacy, be easy to be accepted by the person under inspection, thereby it is this brand-new, fast, the detection means of Noninvasive will be subjected to using widely. and the saliva detection technique also can solve the variety of problems that the urine detection process occurs: the cross reaction between (1) forbidden drug/drugs and the non-prohibited substance, saliva is detected on the one hand and can be got rid of because of taking the false positive results that other non-prohibited substances produce, also can help drug law enforcement personnel and drugs appraiser to distinguish the true and false of drug addict's confession on the other hand, for drugs fighting work brings great convenience; (2) aspect new recruit, new life, civil servant, special practitioner (pilot, driver, work high above the ground person etc.) health check-up, saliva is detected on the one hand can prevent that the experimenter from mingling the manufacturing false positive in sample, attempt escape to be enlisted/the working obligation, can prevent from also on the other hand that the drug addict from arguing to take non-prohibited substance and get by under false pretences.Aspect the specificity that detects saliva, according to the pharmacokinetics principle, the major part that detected of saliva detection is a medicine itself in theory, but not its metabolic product; Therefore the specificity of saliva detection more is better than urine detection.And in the research and application process of reality, because monoclonal antibodies against morphine and morphine-BSA bond, anti-meth monoclonal antibody and meth-BSA bond that this research institute adopts have passed through a large amount of clinical verifications; Therefore from practical application, the specificity that saliva is detected also more is better than urine detection.

Technical scheme:

The product that this project is developed adopts collaurum competition inhibition method immunochromatography technique, and drugs are antigen coated on cellulose nitrate film, and bag is by the monoclonal antibody specific of colloid gold label on glass fibre.When detecting, if do not have morphine, meth or its metabolic product to exist in the sample, the specific antibody of colloid gold label and film district antigen and two resistive connections close, form a nature controlling line C respectively, article one, morphine response line MOR, a meth response line MET show that testing result is that morphine, meth are all negative.If only have morphine or its metabolic product in the sample, and concentration is higher than detection level (15 ± 5ng/ml) time, the drug numerator morphine just combines with the antibody competition of colloid gold label, thereby stop the antibody of colloid gold label to combine with film district antigen, the red response line MOR of its correspondence is disappeared, the nature controlling line C that a redness only occurs shows that testing result is the morphine positive, meth feminine gender.If only have meth or its metabolic product in the sample, and concentration is higher than detection level (50 ± 5ng/ml) time, the drug numerator meth just combines with the antibody competition of colloid gold label, thereby stop the antibody of colloid gold label to combine with film district antigen, the red response line MET of its correspondence is disappeared, nature controlling line C and a morphine response line MOR that a redness only occurs show that testing result is the meth positive, morphine feminine gender.If have morphine and meth or its metabolic product in the sample simultaneously, and concentration is higher than detection level (morphine 〉=15 ± 5ng/ml, during meth 〉=50 ± 5ng/ml), the colloid gold label antibody competition combination that drug numerator morphine and meth are just corresponding with it respectively, thereby stop the antibody of colloid gold label to combine with film district antigen, the red response line MOR and the MET of its correspondence are all disappeared, the nature controlling line C that a redness only occurs shows that testing result is that morphine and meth are all positive.

Embodiment:

This project product throw in on-the-spot on probation and obtain authoritative department and users' approval after, I start the declaration program at corporation plan, and take to promote trade and investment be main, to sell directly to households be the marketing strategy of assisting, product is taken registration certificate and is begun later on to produce in enormous quantities.My company has set up 100,000 grades cleaning at present, is carrying out quality of production system certification and is actively getting in touch clinical testing unit.Enterprise has veteran technical research personnel, has guaranteed the project maturity of technology; And set up marketing center's organizational structure, sell for product is formal and accomplished fluently certain basis.

Description of drawings:

Fig. 1 is that saliva sampler, Fig. 2 are that narcotic saliva detection components, Fig. 3 are, Fig. 4 is that saliva sampling, Fig. 5 are that narcotic saliva detects, Fig. 6 is that narcotic saliva detects, Fig. 7 is the narcotic saliva test card in the saliva sampling.

Claims

1: a kind of saliva reagent that can detect drugs, this product are applicable to army's conscription, college entrance examination enrollment, unit recruitment, civil servant

Employ, driver's health check-up, public place of entertainment are checked, drug abuse generaI investigation and public security bureau, narcotic house and hospital etc. are single

Drugs/forbidden drug the inspection of position.This product is that drugs are antigen coated on cellulose nitrate film, wraps on glass fibre

By the specific antibody of colloid gold label.When detecting, there is not the collaurum mark as having drugs or its metabolic product in the sample

Specific antibody and the film district antigen of note and two resistive connections close two red lines of formation (a response line T, a nature controlling line C),

Show that testing result is negative; As having drugs or its metabolic product in the sample, and concentration is when being higher than detection level, drugs

Molecule just combines with the antibody competition of colloid gold label, thereby stops the antibody of colloid gold label to combine with film district antigen, makes it

Corresponding red response line disappears, and the nature controlling line of a redness only occurs, shows that testing result is positive.

2, technical characterictic: in view of the drugs quick detection reagent that is adopted on the present domestic and international market all is to be that sample is examined with the urine

Survey, defectives such as the present invention can overcome sample dirt that present drugs detect, mingles easily/substitute, the property invaded privacy this

The product that order is developed adopts collaurum competition inhibition method immunochromatography technique, and drugs are antigen coated at cellulose nitrate film

On, bag is by the monoclonal antibody specific of colloid gold label on glass fibre.When detecting, if do not have in the sample

Coffee, meth or its metabolic product exist, and the specific antibody of colloid gold label and film district antigen and two resistive connections close,

Form a nature controlling line C respectively, morphine response line MOR, a meth response line MET show that testing result is that morphine, meth are all negative.