CN101351153A - Biosensor and apparatus for measuring concentration of components - Google Patents
Biosensor and apparatus for measuring concentration of components Download PDFInfo
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- CN101351153A CN101351153A CNA2007800010660A CN200780001066A CN101351153A CN 101351153 A CN101351153 A CN 101351153A CN A2007800010660 A CNA2007800010660 A CN A2007800010660A CN 200780001066 A CN200780001066 A CN 200780001066A CN 101351153 A CN101351153 A CN 101351153A
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Abstract
This invention provides an apparatus for measuring the concentration of components by blood collection through laser beam piercing, which apparatus can prevent the leakage of an offensive smell generated during laser piercing and can improve the convenience of users. The component concentration measuring apparatus comprises a body (2). The body (2) is provided with a laser device, a focusing lens, an analyzer for analyzing components of a body fluid by an enzymatic reaction with an analyte reagent, a laser operating button (5), a display (3), a display switching button (4), a chargeable battery, and an electric circuit for the analysis of the concentration of components by the operation of the laser and the output from the analyzer and provided with a memory for storing analysis results. A slide sheet (6) comprising a film provided on an optical axis of a laser beam and an insertion holder (7) of which the interior is in a hollow state are inserted into the body (2). A biosensor (8) comprising a film provided on the optical axis of a laser beam is set so that the protrusions of the insertion holder (7) are fitted into the opening of the biosensor (8).
Description
Technical field
The present invention relates to a kind of apparatus for measuring concentration of components, its available laser beam pierces through the skin of human body, and the quantity of the various special components of the trace in the biological specimen of determining to be sampled fast and easily, and relate to the biosensor that is used for this apparatus for measuring concentration of components.
Background technology
Traditional biosensor will be described by concept map.
Figure 58 has shown traditional laser aid, and in its one side, the finger tip abdomen is used the laser beam irradiation of traditional laser aid.The inner solid-state laser agitator of reference marker 101 expression, labelling 102 expression main bodys, solid-state laser agitator 101 is arranged on wherein, and power supply and control device are arranged in the controller 103 as independent unit.Lens cap 104 is arranged on the outlet side of the laser beam in the main body 102, and collecting lens 105 is arranged on the inside of lens cap 104, and is assembled by collecting lens 105 from the laser beam output of solid-state laser agitator 101.
For from the finger tip sampling blood, the people's that its blood will be sampled part finger abdomen is placed on the platform 106, towards collecting lens 105.Finger holder 108 is the lateral bending song within it, makes people's finger tip to enter a little, and is fixed to the upper surface of platform 106, makes laser beam can accumulate near the outside of top edge of this holder.
The people's that will be sampled when its blood finger 107 laser beam irradiations, at point of irradiation generation Wicresoft mouth, and blood oozes out.Then, the people's that its blood will be sampled blood is by the biosensor sampling of independent setting, and the special component of the trace in the blood is quantized (for example, referring to Patent Document 1).
In addition, Figure 59 is the decomposition diagram as the glucose sensor of an embodiment of traditional biosensor.On insulated substrate 120, comprise that the silver paste of resin binder and conduction carbon paste are printed, and be formed with the electrode system that comprises lead 112 and 113, measurement electrode 114 and antielectrode 115.And insulating barrier 116 forms by the printing insulating paste.
System for electrode 114 and 115, after the part that polishing is exposed, carboxymethyl cellulose (hereinafter is called CMC, it is a hydrophilic polymers) aqueous solution be coated on the electrode, form the CMC layer thus, and wherein the material that is dissolved in the phosphate buffer solution as the glucoseoxidase (GOD) of enzyme is coated, to cover the CMC layer, forms the conversion zone that comprises the CMC-GOD layer thus.
Shown in Figure 59, substrate 120, the dividing plate of making by resin plate 117 and cover plate 119 these three members in conjunction with and integrate, make each member can have the position relation shown in the dotted line.When integrating, an end of cut-away portions becomes the introducing port 110 of sample solution, and mid portion forms spatial portion 118.In addition, cover plate 119 has the hole, and when integrating, it becomes outlet 111.
When sample solution was blood contact introducing port 110, this blood imported inner from introducing port 110.At this moment, the air in the spatial portion 118 is discharged from outlet 111 apace.Equally, blood is in the electrode surface rapid diffusion, and is full of this spatial portion.Blood dissolves CMC and become viscose, and oxidation reaction takes place by the effect that is carried on the glucoseoxidase on the electrode in the glucose in the blood, produces hydrogen peroxide thus.By applying voltage between the electrode, by this measurement electrode of hydrogen peroxide oxidation of enzyme reaction generation, and the concentration (for example, referring to Patent Document 2) of the corresponding glucose substance of the oxidation current value of following.
On the other hand, when laser beam pierced through skin (laseropuncture), research alleviated the technology of the pain of user as a rule.Figure 60 is the flow chart that shows the operation of traditional laseropuncture device.In traditional laseropuncture device, if the start button of this device is pressed, whether switch is partly pressed in step S1 is determined.Produce sound (being) if desired, this switch is partly pressed, and program advances to step S2, and in this step, for example the sound of music produces from speaker.Then, by push the puncture switch in step S3, program advances to the step S4 that punctures.Then, after the time through fixing period, program advances to step S5, in this step, is stopped from the sound of speaker.
In addition, if switch is partly pressed in step S1, program advances to step S6, and in this step, the puncture switch is pressed.Correspondingly,, do not spread out of from the sound of speaker at step S7, but carried out puncturing (for example, referring to Patent Document 3).Equally, in traditional laseropuncture device, speaker is arranged in this laseropuncture device, and to produce music or sound, it offsets the operation sound in the piercing process.This has alleviated the pain in the piercing process.
Patent documentation 1:JP-A-04-314428
Patent documentation 2:JP-A-01-291153
Patent documentation 3:JP-A-2005-131088
Summary of the invention
The problem to be solved in the present invention
Yet, the problem below existing in traditional laseropuncture device and the bonded apparatus for measuring concentration of components of biosensor.
At first be in the process of laseropuncture skin, user smells the plume abnormal flavour of generation, and feels quite uncomfortable, and plume is stained with on the optical element and is polluted.Laseropuncture skin is by absorbing laser beam energy in the main component moisture of skin histology, by heating or transpiring moisture skin histology being heat-treated.At this moment, produce the plume evaporant in the space.
Skin comprises epidermis, corium and subcutaneous tissue successively from the outside.For sampling blood, must pierce through the capillary tube in the skin mastoid process of skin to the side of the ground floor epidermis of corium, and most of puncture occurs on the epidermis.In skin of palm of hand, particularly for example point meat, the epidermis of skin is thicker, has thickness 1 to 1.5mm, and most skin is horny layer, and its main component is a keratin protein.The volatile material that produces as the aminoacid of the composition of keratin protein is decomposed and is flowing in the plume.Horny layer is made up of the fibrous tissue of keratin protein, and fiber is by the cystine linkage combination, and this key is the key of the sulfur part of the more relatively amino acid cystine that comprises.
Because these aminoacid decompose in evaporation process, have produced volatile sulphur compound or nitrogen compound, especially, the people feels that they are a kind of abnormal flavour.In addition, when these evaporants were attached on the optical element of laser beam, because attachment is to the absorption of laser beam, the transmittance of optical element reduced, and the situation of burning of attachment takes place.
Second problem is the infection problem when blood is sampled by laser aid.For the aggregation laser bundle with skin puncture in the zone below 1 square millimeter, the distance between collecting lens and the skin should be constant with the maintenance of the precision below the 1cm at least.Therefore, in fact, skin is compressed against on the specific fixed structure, and uses laser beam irradiation.Because this structure is repeatedly used by same user, perhaps used by two above users, worry to infect via this structure.In addition, as also as described in the preamble, when using laser aid to puncture, produce plume.Because this plume adheres to, and also has infectious worry.
The 3rd problem is to use the complication in the process.After with the device sampling blood, user should be put blood into and be provided with separately in the pick off, this pick off is connected with display, and quantizes the various special components of the trace in the blood.And when existing a large amount of replacement parts to solve up to now problem, the convenience of user may reduce.
In addition, in the laser irradiation situation of traditional laser aid, the optically focused diameter of laser beam is more than the 0.5mm, and cicatrix can keep more than the week.Although there are individual variation in moisture content of skin and thickness,, therefore radiate excessive laser beam energy for the laser beam pulses of utilizing primary emission punctures reliably stably to take a blood sample.For this reason, in some cases, because the paracentesis depth of skin is too dark, it is strong that pain becomes.
Owing to inventor's research, produced a kind of optically focused diameter of laser beam of preventing from scar, this optically focused diameter is below the 0.15mm, and is preferably below the 0.1mm.In addition, the inventor has found if the optically focused diameter is 0.5mm (traditional), and then the individual variation of the required laser beam energy of blood sampling is about twice, and if the optically focused diameter by below the 0.15mm, then become about tens percent.And the inventor finds, if the optically focused diameter is below the 0.15mm, the required laser beam energy of blood sampling can also be the quite little energy of about tens mJ.
Use laseropuncture to carry out blood sampling from skin owing to study in great detail, the inventor has found the following fact.Figure 61 is the sketch map of skin.Skin from outside comprise epidermis and corium successively, for sampling blood, must destroy the good capillary tube of skin mastoid process in the corium.Yet, the free teleneuron that detects pain also be present in the skin mastoid process near.Epidermis comprises five kinds of layers, comprises the horny layer on the outmost surface.
Usually, blood sampling is to carry out on the skin of finger tip or palm.This is because the blood glucose levels in the capillary tube of finger tip or palm can be measured by no time lag of ground, for example when measuring diabetes patient's blood glucose levels.It is said that it is 1 to 1.5mm epidermis, particularly horny layer that the finger tip of the most general blood sampling point has thickness.In addition, this horny layer is a thanatogenic tissue, and has the outward appearance that is different from tissue alive, for example has the skin mastoid process of corium capillaceous.
The inventor finds during along shape that its depth direction is observed skin in the laseropuncture process, for example when the surface of skin is used laser gathering patterned illumination, the cross sectional shape that the main epidermis of being made up of horny layer has substantial cylindrical, and corium has conical cross sectional shape.
In order to realize the hypodynia laser irradiating method, the degree of depth of cross sectional shape in hole of importantly controlling conical laseropuncture is to essential minimum-depth.If the degree of depth is excessive, then stimulates free teleneuron, make user feel that pain and the hemorrhage probability that needs that surpasses uprise.In addition, if corium is pierced through dearly, can produce the evaporation and the reacting by heating of dermal tissue along the shock wave (compressional wave) of the direction opposite with evaporation because the optical absorption of laser beam energy makes, this also can be changed into a kind of pain.
On the other hand, as mentioned above, in traditional laseropuncture device, alleviate pain in the piercing process in the laseropuncture device, and offset operation sound in the piercing process by producing the music or the sound by speaker is set.Yet if user is always listened identical music etc., he may get used to this music etc.As a result, the consciousness of user can't focus on this music etc., and the pain in the piercing process can not alleviate.
In addition, owing to always occur in same time after the puncture switch is pressed on opportunity of laseropuncture, user can be unconsciously to the opportunity of being careful laseropuncture, so the pain in the piercing process can not alleviate.
In addition, in traditional laseropuncture device, the optically focused diameter or the time width of laser pulse bundle can design equably.Yet because the optimum condition of blood sampling is different for each user reliably, the variable-width of laser pulse beam energy is set up, and user individually is provided with the laser pulse beam energy.For this reason, in order to check the laser beam energy of sampling blood for user, at first, therefore his laseropuncture that should experimentize can not use single laser puncture carrying out blood sampling.In addition, because the condition of checking from day to day changes, this condition should be checked at every turn.
Of the present invention making is in order to solve these traditional problems.Therefore, an object of the present invention is to provide a kind of biosensor and apparatus for measuring concentration of components, it can make user avoid feeling abnormal flavour in using the laser beam piercing process, and carries out simple and easy measurement and do not worry to use and cause infection.
In addition, the purpose of this invention is to provide a kind of laser irradiating method of laseropuncture device, it can carry out stable blood sampling and not stay cicatrix, and pain is very little in piercing process.
In addition, the objective of the invention is to provide a kind of laseropuncture device and laseropuncture method, wherein user not can be appreciated that the opportunity of laseropuncture, and the pain detection level of user is lower, and the pain in the piercing process is very little.
In addition, the purpose of this invention is to provide a kind of laseropuncture device and laseropuncture method, it can be for user sampling blood and do not need experiment puncture reliably.
And, the purpose of this invention is to provide the mode of operation of laseropuncture device, particularly, the degradation of monitoring flash lamp, it is the main degeneration factor of this device.In addition, another purpose is the access times that will monitor the laseropuncture device.
The means of dealing with problems
Apparatus for measuring concentration of components of the present invention comprises: main body, its have at least laser aid, this laser beam of emission laser beam beam condensing unit, body fluid composition analytical equipment and show display device by this analytical equipment result calculated; Sheet; Insertion body with the opening that does not cover this laser beam; And reagent paper.Here, main body is provided with opening along the optical axis direction of laser beam.Opening is equipped with the insertion body, and an end of this insertion body is equipped with reagent paper.Sheet provides the film between beam condensing unit and insertion body, and this film is not punctured by laser beam.
By this configuration, because user can not smell the abnormal flavour in the plume that produces in the laseropuncture process, and the reagent paper of the sampled point of each puncture replacing contact user, needn't worry to infect, and, can realize simple and easy use because user can utilize a mechanism to sample and analyze.
In addition, in apparatus for measuring concentration of components of the present invention, reagent paper is provided with the composition measurement electrode, insert body and be provided with electrode, and the composition measurement of reagent paper with the electrode of electrode and insertion body by being electrically connected with reagent paper is chimeric.
By this configuration, in the component analysis of the bodily fluid sampling by laseropuncture, user can carry out component analysis and not remove reagent paper and reinstall it and be used for component analysis once more, and simple thus and be easy to use the possibility that becomes.
In addition, in apparatus for measuring concentration of components of the present invention, analytical equipment is analyzed the composition of this body fluid by the enzyme reaction of sample reagent.
By this configuration, because user can not smell the abnormal flavour in the plume that produces in the laseropuncture process, and the reagent paper of the sampled point of each puncture replacing contact user, needn't worry to infect, and, can realize simple and easy use because user can utilize a mechanism to sample and analyze.
And in apparatus for measuring concentration of components of the present invention, analytical equipment is Optical devices of analyzing the composition of this body fluid by the chromogenic reaction of sample reagent.
By this configuration,, can obtain simple and easy use in a mechanism because user can sample and analyze.
In addition, in apparatus for measuring concentration of components of the present invention, reagent paper provides the film that is punctured by laser beam on the optical axis that is positioned at laser beam.
By this configuration, the plume that produces in the laseropuncture process passes the hole of puncture and discharges from the skin of user.Therefore, this plume can not leak into user, so user can be prevented from smelling the abnormal flavour in the plume that produces in the laseropuncture process.
In addition, in apparatus for measuring concentration of components of the present invention, reagent paper also provides the film that is not punctured by laser beam on the optical axis that is positioned at laser beam.
By this configuration, the plume that produces in the laseropuncture process passes the hole of puncture and discharges from the skin of user, and is limited in the reagent paper.Therefore, plume can not leak into user, so user can be prevented from smelling the abnormal flavour in the plume that produces in the laseropuncture process.
In addition, in apparatus for measuring concentration of components of the present invention, reagent paper also has the function as sheet.
By this configuration, user needn't have different with managerial structure two kinds of sheets and biosensor, and the convenience of user can be enhanced.
In addition, in apparatus for measuring concentration of components of the present invention, reagent paper also provides the film that is used as sheet on the optical axis that is positioned at laser beam, and when as sheet, this film is not punctured by laser beam.
By this configuration, because user needn't have different with managerial structure two kinds of sheets and biosensor, and change this sheet needn't use the time at every turn, the convenience of user can be enhanced.
And in apparatus for measuring concentration of components of the present invention, the insertion body is fitted to main intravital sidepiece and is provided with unsymmetric structure.
By this configuration, user can not made a mistake direction of insertion, and the therefore simple and easy possibility that becomes of using.
And apparatus for measuring concentration of components of the present invention provides a structure, wherein reagent paper and insert chimeric between the body and undertaken by unsymmetric structure.
By this configuration, user can not made a mistake the cooperation direction, and the therefore simple and easy use possibility that becomes.
And apparatus for measuring concentration of components of the present invention provides a structure, wherein reagent paper and to insert chimeric between the body be chimeric carrying out between opening by reagent paper and the projection of inserting body.
By this configuration, user can not made a mistake the cooperation direction, and it can not skid off.Therefore, the simple and easy use possibility that becomes.
And, in apparatus for measuring concentration of components of the present invention, the film of sheet, insert body and reagent paper film at least a portion or all be provided with deodorization functions.
By this configuration, the abnormal flavour in the plume that produces in the laseropuncture process can be eliminated.
And, in apparatus for measuring concentration of components of the present invention, reagent paper, insert body and reagent paper film at least a portion or all be provided with antibacterial functions.
By this configuration, can prevent the infection when user uses.
Biosensor of the present invention is to be used for the puncture biosensor of composition of the sample specimen of collecting of analysis and utilization laser beam.Here, this pick off forms the sample service duct, and the sample specimen of being supplied is drawn onto between this first and second substrate by this sample service duct; By being fitted between this first and second substrate, filter and be arranged in this sample service duct with the reagent of composition reaction in this sample specimen; And leading to the air outside hole from this sample service duct is arranged in this second substrate.Reagent has enzyme and chromogen, and it specifically reacts with this composition, and partly or entirely shared opening is arranged on the outside of the sample service duct in first and second substrates, and any one opening at least of first and second substrates is provided with film.
By this configuration, the plume that comprises abnormal flavour that is produced by laser beam in piercing process is limited in the sensor board, can suppress plume and leak into the outside.
In addition, in biosensor of the present invention, film has deodorization functions.
By this configuration, the odor pollutant in the plume that is produced by laser beam in the piercing process can be eliminated.
And in biosensor of the present invention, first and second substrates also are provided with the part or all of shared opening outside this opening.
By this configuration, can set up reliably and being connected of the laseropuncture device that comprises laser oscillator.Thus, the relative distance between laser oscillator and the pick off can be determined, and the shape variable in laseropuncture hole must be stablized.
Laseropuncture biosensor of the present invention is to be used for the puncture biosensor of composition of the sample specimen of collecting of analysis and utilization laser beam.This pick off forms the sample service duct, and the sample specimen of being supplied is drawn onto between this first and second substrate by this sample service duct; At least by being fitted between this first and second substrate, be arranged in this sample service duct with the reagent of composition reaction in this sample specimen; And leading to the air outside hole from this sample service duct is arranged in this second substrate.Pick off has electrode system, and this electrode system comprises measurement electrode and electrode galvanic couple at least, and reaction is detected by electrode system.Partly or entirely shared opening is arranged on the outside of the sample service duct in first and second substrates, and any one opening at least of first and second substrates is provided with film.
By this configuration, the plume that comprises abnormal flavour that laser beam produces in the piercing process is limited in the sensor board, and can suppress plume and leak into the outside.
In addition, in biosensor of the present invention, film has deodorization functions.
By this configuration, the odor pollutant in the plume that is produced by laser beam in the piercing process can be eliminated.
And in biosensor of the present invention, first and second substrates also are provided with the part or all of shared opening outside this opening.
By this configuration, can set up reliably and being connected of the laseropuncture device that comprises laser oscillator.Thus, the relative distance between laser oscillator and the pick off can be determined, and the shape variable in laseropuncture hole must be stablized.
And with in the biosensor, first and second substrates also are provided with at least two the part or all of openings of sharing outside this opening, make electrode system to expose at laseropuncture of the present invention.
By this configuration, can set up reliably and being connected of the equipment that comprises laser oscillator and analytical equipment.Thus, the relative distance between laser oscillator and the pick off can be determined, and the shape variable in laseropuncture hole must be stablized.And, can be electrically connected pick off and device, monitor connection status thus.
In addition, apparatus for measuring concentration of components of the present invention comprises: main body, its have at least laser aid, this laser beam of emission laser beam beam condensing unit, body fluid composition analytical equipment and show display device by this analytical equipment result calculated; Sheet; Insertion body with opening that laser beam passes; And reagent paper.Main body is provided with opening along the optical axis direction of laser beam.Opening is equipped with the insertion body, and an end of this insertion body is equipped with reagent paper.Sheet is between beam condensing unit and insertion body, and this sheet has the function of reagent paper.
By this configuration, because user can not smell the abnormal flavour in the plume that produces in the laseropuncture process, and the pollution as the optical element of beam condensing unit that is caused by plume can not realize simple and easy use, and user can use a kind of consumable goods to manage sheet and pick off.
In addition, this sheet provides the film on the optical axis that is positioned at this laser beam, and this film is not punctured by this laser beam as this sheet the time, and is punctured by this laser beam when as this reagent paper.
By this configuration, because user can not smell the abnormal flavour in the plume that produces in the laseropuncture process, and the pollution as the optical element of beam condensing unit that is caused by plume can not realize simple and easy use, and user can use a kind of consumable goods to manage sheet and pick off.
And, sheet provide on the optical axis that is positioned at this laser beam be used as this reagent paper the time film that punctured by this laser beam, and provide on the optical axis that is positioned at this laser beam be used as this sheet the time film that punctured by this laser beam.
By this configuration, because user can not smell the abnormal flavour in the plume that produces in the laseropuncture process, and the pollution as the optical element of beam condensing unit that is caused by plume can not realize simple and easy use, and user can use a kind of consumable goods to manage sheet and pick off.
And, also provide detection lug to be inserted into main functions.
And, can prevent that user from forgetting the insertion of sheet, and correspondingly, can prevent the pollution that causes by plume reliably as the optical element of beam condensing unit.
And it is that the Mechanical Contact of the insertion by sheet is operated and carried out that detection lug is inserted into main functions.
By this configuration, can prevent that user from forgetting the insertion of sheet, and correspondingly, can prevent the pollution that causes by plume reliably as the optical element of beam condensing unit.
And it is by being arranged on being electrically connected to fetch and carrying out of electrode in the sheet that detection lug is inserted into main functions.
By this configuration, can prevent that user from forgetting the insertion of sheet, and correspondingly, can prevent the pollution that causes by plume reliably as the optical element of beam condensing unit.
And at least one or two in the film of sheet and the insertion body are provided with deodorization functions.
By this configuration, the abnormal flavour in the plume that produces in the laseropuncture process can not eliminated.
And at least one or two that insert in keeper and the sheet are provided with antibacterial functions.
By this configuration, can prevent any infection when user uses.
Puncture according to the present invention is mounted in the puncture that punctures thus on the laseropuncture device that utilizes laser beam irradiation skin.This adapter comprises: the ducted body that has opening two ends; Be used for sealing first film and second film of the opening of these two ends with setting.First film absorbs laser beam and is punctured, the second film transmission laser bundle and do not absorb laser beam.
According to above-mentioned configuration, form enclosed space by first film, ducted body and second film.Thus, when utilization is pressed in first film on the skin when carrying out laseropuncture, in the space that the plume of generation can be limited in sealing.Therefore, user can carry out laseropuncture and can not smell a strange smell and feel under the weather.In addition, change puncture during by each use, do not worry the infection in the use, and puncture can be carried out simple and easyly.
In addition, in puncture according to the present invention, ducted body has the projection that is formed on around first film.
According to above-mentioned configuration and since projection will push predetermined laseropuncture point around, can alleviate the stimulation that user is felt in the laseropuncture process.And, when by puncture blood when being sampled, sampling blood stably.
In addition, in puncture according to the present invention, at least one in first film, ducted body and second film is provided with deodorization functions.
According to above-mentioned configuration, the abnormal flavour in the plume that produces in the laseropuncture process can be eliminated.
In addition, in puncture according to the present invention, first film is provided with antibacterial functions.
According to above-mentioned configuration, can prevent infection via puncture.
And laseropuncture device according to the present invention comprises separable according to puncture of the present invention, and the central shaft of this ducted body and laser beam axis are set to overlap each other.
According to above-mentioned configuration, form enclosed space by first film, ducted body and second film.Thus, when utilization is pressed in first film on the skin when carrying out laseropuncture, in the space that the plume of generation can be limited in sealing.Therefore, user can carry out laseropuncture and can not smell a strange smell and feel under the weather.In addition,, when each the use, be replaced, needn't worry to infect, and puncture can be carried out simple and easyly owing to will contact the puncture of the skin of user according to above-mentioned configuration.
By setting laser irradiation condition that is used for laseropuncture corium that differs from one another and the laser irradiation condition that is used for the laseropuncture epidermis, with utilize dissimilar laser pulse beam energies repeatedly to shine, the present invention has realized stable blood sampling and almost painless laseropuncture.
Especially, the time width of the first laser pulse bundle of puncture epidermis is set up the second laser pulse bundle that equals or be longer than puncture corium, and laser beam energy is set up greater than the second laser pulse bundle.Here, the laser pulse bundle of puncture epidermis being divided into a plurality of laser pulse bundles is effective for stable blood sampling.In this embodiment, when the illuminate condition of laser pulse bundle of the laser pulse bundle of puncture epidermis and puncture corium is compared to single laser pulse bundle, they can be identical, even or the width of the laser pulse bundle of puncture assembling is longer than and laser beam energy greater than each laser pulse bundle of puncture epidermis.
In addition, laser irradiating method according to the present invention is a kind ofly to utilize laser pulse bundle irradiation to comprise that the skin of epidermis and corium carries out the laser irradiating method of laseropuncture thus.The method comprising the steps of: radiation be used to the to puncture laser pulse bundle of this epidermis; With radiation be used for the puncturing laser pulse bundle of corium of point of puncture of this epidermis.
According to above-mentioned configuration, the laser pulse bundle of the corium that is used to puncture shines the position with the laser pulse Shu Xiangtong of the epidermis that is used to puncture, and mortar shape puncturing hole is formed in the corium.Therefore, corium is not punctured dearly, can not stay cicatrix, and does not almost have pain in the piercing process, and can carry out stable blood sampling.
In addition, in laser irradiating method according to the present invention, the time width of laser pulse bundle of this epidermis of being used to puncture equals or is longer than to be used to the laser pulse bundle of corium that punctures, and the energy of the laser pulse bundle of this epidermis that is used to puncture is greater than the laser pulse bundle of the corium that is used to puncture.
According to above-mentioned configuration, the degree of depth of the cross sectional shape in the laseropuncture hole in the corium can be controlled to necessary minima, and can not stay any cicatrix, and does not almost have pain in the piercing process, and can carry out stable blood sampling.
In addition, in laser irradiation condition according to the present invention, the time width of the laser pulse bundle of the epidermis that is used to puncture is 100 to 400 μ s, and the time width of the laser pulse bundle of the corium that is used to puncture is 50 to 300 μ s.
According to above-mentioned configuration,, can not stay cicatrix, and almost not have pain in the piercing process, and can carry out stable blood sampling because corium is not punctured dearly.
In addition, in laser irradiating method according to the present invention, the difference that the time width of the laser pulse bundle of the epidermis that is used to puncture and being used to punctures between the time width of laser pulse bundle of corium is 50 to 200 μ s.
According to above-mentioned configuration,, almost do not have pain in the piercing process, and can carry out stable blood sampling because the people that its blood is sampled awares a puncture.
In addition, in laser irradiating method according to the present invention, the irradiation that the laser pulse bundle of the epidermis that is used to puncture and being used to punctures between the laser pulse bundle of corium is spaced apart below the 500ms.
According to above-mentioned configuration,, almost do not have pain in the piercing process, and can carry out stable blood sampling because the people that its blood is sampled awares a puncture.
In addition, in laser irradiating method according to the present invention, the laser pulse bundle of the epidermis that is used to puncture and being used to puncture the energy summation of laser pulse bundle of corium be every square centimeter 100 to 300J.
According to above-mentioned configuration, the energy that is used for the laser pulse bundle of blood sampling can be minimized, and can not stay cicatrix, and does not almost have pain in the piercing process, and can carry out stable blood sampling.
In addition, in laser irradiating method according to the present invention, the energy of the laser pulse bundle of the corium that is used to puncture be used to puncture epidermis and being used to puncture corium the laser pulse bundle the energy summation 10 to 40%.
According to above-mentioned configuration, the degree of depth of the cross sectional shape in the taper laseropuncture hole in the corium can be controlled to necessary minima, and can not stay any cicatrix, and does not almost have pain in the piercing process, and can carry out stable blood sampling.
In addition, in laser irradiating method according to the present invention, the optically focused diameter of laser pulse bundle is below the 0.15mm.
According to above-mentioned configuration, the individual variation in the energy of the laser pulse bundle that blood sampling is required can be set to less, can not stay any cicatrix, and does not almost have pain in the piercing process, and can carry out stable blood sampling.
In addition, in laser irradiating method according to the present invention, the laser pulse bundle of the epidermis that is used to puncture comprises a plurality of laser pulse bundles.
According to above-mentioned configuration,, can not stay any cicatrix, and almost not have pain in the piercing process, and can carry out stable blood sampling because the laser pulse bundle of the epidermis that is used to puncture can fine be controlled according to the individual variation of skin thickness, water content etc.
In addition, in laser irradiating method according to the present invention, the time width of each that a plurality of laser pulses of the epidermis that is used to puncture are intrafascicular is 100 to 400 μ s.
According to above-mentioned configuration,, almost do not have pain in the piercing process, and can carry out stable blood sampling because the people that its blood is sampled awares a puncture.
In addition, in laser irradiating method according to the present invention, the irradiation of a plurality of laser pulse bundles of the epidermis that is used to puncture is spaced apart below the 500ms.
According to above-mentioned configuration,, almost do not have pain in the piercing process, and can carry out stable blood sampling because the people that its blood is sampled awares a puncture.
In addition, in laser irradiating method according to the present invention, a plurality of laser pulse bundles of the epidermis that is used to puncture and being used to puncture the energy summation of laser pulse bundle of corium be every square centimeter 5 to 100J.
According to above-mentioned configuration, the energy that is used for the laser pulse bundle of blood sampling can be minimized, and can not stay cicatrix, and does not almost have pain in the piercing process, and can carry out stable blood sampling.
Laseropuncture device according to the present invention is a kind of laseropuncture device that utilizes laser beam irradiation skin to puncture thus, and it comprises the periodicity flexible piezoelectric sound-generating devices that produces periodicity sound.Puncture is carried out during producing this periodicity sound.
According to above-mentioned configuration, by producing periodically sound, the consciousness of user is transferred, and it makes user not recognize the moment of puncture.Thus, user feels that the threshold level of pain reduces.Because laseropuncture carries out under this state, can reduce the pain in the piercing process.
In addition, in laseropuncture device according to the present invention, periodically sound is that its mid frequency is with 20 to 100Hz dull multiple sound.
According to above-mentioned configuration carry out because puncture is drawn towards in the consciousness of user under the state of dull multiple sound, and user about the detection level of pain by step-down, the pain in the piercing process can alleviate.
In addition, in laseropuncture device according to the present invention, dull multiple sound comprises the operation sound and the electromotor sound of pump.
According to above-mentioned configuration, because the consciousness of user is drawn towards dull multiple sound, for example operation sound of pump and electromotor sound, by step-down, the pain in the piercing process can alleviate user about the detection level of pain.
In addition, in laseropuncture device according to the present invention, periodically sound is to have the sound that per minute impacts 60 to 208 repetition beat.
According to above-mentioned configuration be drawn towards under the state of dull multiple sound and carry out because puncture is a consciousness at user, and user about the detection level of pain by step-down, the pain in the piercing process can alleviate.
In addition, in laseropuncture device according to the present invention, sound with repetition beat comprise metronomic sound, mechanical switch ON/OFF sound and knock the sound of keyboard, and user about the detection level of pain by step-down, the pain in the piercing process can alleviate.
According to above-mentioned configuration, since puncture be drawn towards in the consciousness of user the sound with repetition beat, metronomic sound, mechanical switch ON/OFF sound and knock under the state of sound of keyboard and carry out, and by step-down, the pain in the piercing process can alleviate user about the detection level of pain.
In addition, in laseropuncture device according to the present invention, this is sound generating apparatus generation tonequality or different polytype periodicity sound of cycle periodically, and change the type of the periodicity sound that produces when at every turn puncturing randomly.
According to above-mentioned configuration, because the periodicity sound randomly changing of different a plurality of types of tonequality or cycle, user can not got used to ambient sound, and the consciousness of user is shifted from puncture.Therefore, even under the situation that the laseropuncture device is used, the effect that alleviates the pain in the piercing process can be held.
In addition, in laseropuncture device according to the present invention, the time from the generation of periodicity sound to puncture changes when each puncture randomly.
In addition, owing to change randomly when puncturing to the time of puncturing from the generation of periodicity sound at every turn, user can not predict the opportunity of laseropuncture, and the pain during the puncture can be alleviated.
In addition, laseropuncture method according to the present invention is a kind of laseropuncture method of utilizing laser beam irradiation skin to puncture thus, punctures thus.The method comprising the steps of: select and produce predetermined periodicity sound from different polytype periodicity sound of tonequality or cycle, and puncturing after through the time at random from the generation of predetermined periodicity sound.
According to above-mentioned configuration, because tonequality or different polytype periodicity sound of cycle change randomly, user can not predicted the opportunity of laseropuncture, and the pain in the piercing process can be alleviated.
Laseropuncture device according to the present invention is a kind of laseropuncture device that utilizes laser beam irradiation skin to puncture thus.This laseropuncture device comprises adjusting device, and its basis is regulated the energy of this laser pulse bundle by the moisture content of skin of the moisture measurement sensor measurement of measurement moisture content of skin.
According to above-mentioned configuration, can be set according to the energy of the suitable laser pulse bundle of moisture content of skin.Therefore, the puncture that do not experimentize, or user oneself do not carry out the setting of laser pulse bundle, and almost do not have the reliable blood sampling of pain to carry out according to individual variation, daily fluctuation and time fluctuation.
In addition, laseropuncture device according to the present invention comprises the moisture measurement pick off.In addition, in laseropuncture device according to the present invention, the moisture measurement pick off comprises sensor electrode, and it is arranged on the surface of laseropuncture device main body, to measure moisture content of skin.
According to above-mentioned configuration, owing to be used to measure the surface that the sensor electrode of moisture content of skin is arranged on the laseropuncture device main body, moisture content of skin can easily be measured.
In addition, laseropuncture device according to the present invention comprises holding device, and it keeps having the puncture medicated cap of moisture measurement pick off separably.This puncture medicated cap is included in first film and second film that two ends have the ducted body of opening and are set to seal the opening of these two ends.This first film transmission laser bundle also absorbs laser beam and is punctured, and this second film transmission laser bundle and do not absorb laser beam.The moisture measurement pick off comprises the sensor electrode on the same level that is arranged on first film, to measure moisture content of skin.
According to above-mentioned configuration because the puncture medicated cap comprises the sensor electrode of measuring moisture content of skin, can carry out simple, easily, blood sampling reliably, by changing the medicated cap that punctures when each the use, and do not worry the infection between the operating period.
In addition, laseropuncture device according to the present invention comprises the setting button of the attribute of importing user.Adjusting device is according to regulating the energy of laser pulse bundle from setting button inputted attribute.
According to above-mentioned feature, according to from setting the attribute of button inputted user, for example age, sex, ethnic group or skin matter are regulated the energy of laser pulse bundle.Therefore, can be according to the ideal laser pulse beam energy of the condition enactment of skin, and can almost not have the reliable blood sampling of pain.
In addition, laseropuncture device according to the present invention comprises the humidity sensor of the humidity of measurement environment air.Adjusting device is regulated the energy of laser pulse bundle according to the humidity of the surrounding air of humidity sensor measurement.
According to above-mentioned configuration since the laser pulse bundle according to and the humidity of the surrounding air that is closely related very much of moisture content of skin be conditioned, can set the minimum laser pulsed beams energy that is suitable for blood sampling exactly.
In addition, laseropuncture method according to the present invention is a kind of laseropuncture side that utilizes laser beam irradiation skin to puncture thus.This method comprises the measurement moisture content of skin; And the energy of regulating this laser pulse bundle according to the moisture content of skin of measuring.
According to above-mentioned configuration, can be set according to the suitable laser pulse beam energy of moisture content of skin.Therefore, the puncture that do not experimentize, or user oneself do not carry out the setting of laser pulse bundle, and almost do not have the reliable blood sampling of pain to carry out according to individual variation, daily fluctuation and time fluctuation.
In addition, insertion keeper of the present invention is a kind of insertion keeper that utilizes on the laseropuncture device that laser beam irradiation skin punctures thus that is installed in.This insertion keeper comprises: the ducted body that has opening two ends; Be set to seal an end of this opening, and transmission laser bundle and do not absorb the film of laser beam; Be set to seal the biosensor of the other end of this opening; And be arranged on the outer peripheral face of this ducted body or inner peripheral surface to be electrically connected the electrode of this laseropuncture device and this biosensor.
According to above-mentioned configuration, insert keeper and constitute, but have an insertion keeper to constitute now by biosensor, insertion keeper and three elements of slide plate.Therefore, have an advantage, when user used this device, the part that change can be only one.
In addition, in insertion keeper of the present invention, finger holder film is formed in the part surface of this biosensor, and sample reagent service duct opening is formed in the side of this biosensor.
In addition, in insertion keeper of the present invention, biosensor has measurement electrode and the electrode galvanic couple that is formed in the reagent layer.
In addition, insertion keeper of the present invention is a kind of insertion keeper that utilizes on the laseropuncture device that laser beam irradiation skin punctures thus that is installed in.This insertion keeper comprises: by crooked biosensor be connected the ducted body that the end of biosensor forms; Be set to seal an end of this ducted body, and transmission laser bundle and do not absorb the film of laser beam; Be set to seal the other end of this ducted body, and the film that absorbs laser beam and be punctured; And be arranged on the outer peripheral face of this ducted body or inner peripheral surface to be electrically connected the electrode of this laseropuncture device and this biosensor.
In addition, laser irradiating method of the present invention is a kind of laser irradiating method that utilizes the laser pulse bundle to carry out laseropuncture thus via film irradiation skin.The method comprising the steps of: radiation be used to the to puncture first laser pulse bundle of this film; And radiation is used for the second laser pulse bundle in the point of puncture skin puncture of this film.
In addition, in laser irradiating method of the present invention, the intensity of the first laser pulse bundle is weaker than the intensity of the second laser pulse bundle.
According to above-mentioned configuration, in film, form through hole by the first laser pulse bundle, and when having individual variation in the laseropuncture condition of skin, can allow stable blood sampling by regulating the second laser pulse bundle.
In addition, biosensor of the present invention is a kind of biosensor that utilizes on the laseropuncture device that laser beam irradiation skin punctures thus that is installed in.This biosensor comprises: first substrate, have attaching thereon reagent layer and have first opening that laser beam passes; Second substrate is set to predetermined space in the face of this first substrate, has second opening that should first opening, and has and be positioned at than the airport of this reagent layer further from the position of this second opening; And the sample reagent service duct, be formed at from this first opening to this reagent layer this first substrate and this second substrate.
According to above-mentioned configuration, user can be supplied blood as the target that will measure to biosensor, even he does not enter his/her finger the opening of sample reagent service duct behind laseropuncture.
In addition, in biosensor of the present invention, the optical axis of this laser beam is set to be parallel to the centrage of this first opening, and is positioned at the position than more close this sample reagent service duct of the centrage of this first opening.
According to above-mentioned configuration, because the more close sample reagent service duct of blood that oozes out but not more close sensor openings center can more effectively arrive reagent layer by capillarity.
In addition, laseropuncture device of the present invention is the laseropuncture device that a kind of laser beam irradiation skin that utilizes flash lamp to encourage punctures thus.This laseropuncture device comprises optical pickocff, and it detects the degradation of the light emission of flash lamp with the monitoring flash lamp.
According to above-mentioned configuration, can point out the service time of user laseropuncture device, and can increase the reliability of sting device.
In addition, laseropuncture device of the present invention comprises informing device, and it informs the message of this matching requirements maintenance of user based on the access times of the device of photoelectric sensor detection.
According to above-mentioned configuration, can point out the user service time according to access times, and can be according to the access times in some cycles, allow the people who bears medical expense, for example the diabetes patient bears medical expense etc.
Effect of the present invention
The invention provides a kind of apparatus for measuring concentration of components, it utilizes the laser beam skin puncture to sample a small amount of body fluid and measure the composition of body fluid by the chromogenic reaction of utilizing sample reagent.In this apparatus for measuring concentration of components, main body is provided with the beam condensing unit of laser aid, laser beam, Optical devices, the analytical equipment of composition and the display device of analysis result of analysis chromogenic reaction at least.Main body has along the opening of the optical axis of laser beam.Be provided with not the sheet of the film that is punctured by laser beam, and the insertion body with the opening that does not cover laser beam inserts in the opening separably.And the reagent paper of the composition of analysing body fluid is set up to be entrenched in and inserts in the body.Thus, can provide apparatus for measuring concentration of components with following effect.Promptly, because user can not smell the abnormal flavour in the plume that produces in the laseropuncture process, and the reagent paper of the sampled point of each sampling replacing contact user, needn't worry to infect, and, can realize simple and easy use because user can utilize a mechanism to sample and analyze.
In addition, the invention provides a kind of apparatus for measuring concentration of components, it utilizes the laser beam skin puncture to sample a small amount of body fluid and measure the composition of body fluid.In this apparatus for measuring concentration of components, main body is provided with the beam condensing unit of laser aid, laser beam, the analytical equipment of composition and the display device of analysis result at least.Main body has along the opening of the optical axis of laser beam.Be provided with not the sheet of the film that is punctured by laser beam, and the insertion body with the opening that does not cover laser beam inserts in the opening separably.And the reagent paper of body fluid is set up to be entrenched in and inserts in the body.Thus, can provide apparatus for measuring concentration of components with following effect.Promptly, because user can not smell the abnormal flavour in the plume that produces in the laseropuncture process, and the reagent paper of the sampled point of each sampling replacing contact user, needn't worry to infect, and, can realize simple and easy use because user can utilize a mechanism to sample and analyze.
In addition, according to the present invention, be provided with the opening of the substrate that passes pick off, and film is set to cover this opening.Thus, laseropuncture can have an effect with biosensor, that is, the plume that comprises abnormal flavour that produces in the laseropuncture process can be prevented from leaking into the outside.
In addition, the invention provides a kind of apparatus for measuring concentration of components, it utilizes the composition of laser beam skin puncture with sample a small amount of body fluid and measurement body fluid.Apparatus for measuring concentration of components comprises main body, this main body be provided with at least laser aid, the laser beam of emission laser beam beam condensing unit, body fluid composition analytical equipment and show display device by the analytical equipment result calculated; Sheet; Insertion body with the opening that does not cover laser beam; And reagent paper.This main body is provided with opening along the optical axis direction of laser beam.Opening is equipped with the insertion body, and an end of this insertion body is equipped with reagent paper.Sheet is between beam condensing unit and insertion body, and sheet has the function of reagent paper.Thus, can provide a kind of apparatus for measuring concentration of components with following effect.Promptly, because user can not smell the abnormal flavour in the plume that produces in the laseropuncture process, also can prevent to be polluted by plume as the optical element of beam condensing unit, and the reagent paper of the sampled point of contact user is changed in each sampling, needn't worry to infect, and because user can manage sheet and reagent paper at least with a kind of consumable goods, and user can utilize a mechanism to sample and analyze, and can realize simple and easy use.
In addition, according to the present invention, can form the space of sealing by first film, ducted body and second film.Thus, when using first film that is pressed on the skin to carry out laseropuncture, in the space that the plume that is produced can be limited in sealing.Therefore, user can carry out laseropuncture and can not smell a strange smell and feel under the weather.
According to the present invention, the laser pulse bundle of the corium that is used to puncture is transmitted into the position with the laser pulse Shu Xiangtong of the epidermis that is used to puncture, and mortar shape puncturing hole is formed in the corium.Therefore, corium is not punctured dearly, can not stay cicatrix, and the pain in the piercing process is very little, and can carry out stable blood sampling.
According to the present invention, by producing periodically sound, the consciousness of user is transferred, and user is caught not note opportunity of puncturing.Thus, the threshold level step-down of user feels pain.Because laseropuncture is to carry out in this state, the pain in the piercing process is reduced.
In addition, according to the present invention, can set suitable laser pulse beam energy according to moisture content of skin.Therefore, the puncture that do not experimentize, or user oneself do not carry out the setting of laser pulse bundle, and can carry out the very little reliable blood sampling of pain according to individual variation, daily fluctuation and time fluctuation.
And, according to the present invention, the service time of user laseropuncture device can be pointed out, and the reliability of sting device can be increased.In addition, can point out the user service time according to access times, and can be according to the access times in some cycles, allow the people who bears medical expense, for example the diabetes patient bears medical expense etc.
Description of drawings
Fig. 1 is the top view of the apparatus for measuring concentration of components in the first embodiment of the present invention;
Fig. 2 is the internal structure sketch map of the apparatus for measuring concentration of components of the first embodiment of the present invention of observing from the side;
Fig. 3 is the lower interior portion structural representation from the apparatus for measuring concentration of components of the first embodiment of the present invention of top view;
Fig. 4 is the insertion keeper in the first embodiment of the present invention;
Fig. 5 is the plane graph and the sectional drawing of the slide plate in the first embodiment of the present invention;
Fig. 6 is the plane graph and the sectional drawing of the biosensor in the first embodiment of the present invention;
Fig. 7 is the zoomed-in view of the laseropuncture point in piercing process;
Fig. 8 is the zoomed-in view of the laseropuncture point in piercing process;
Fig. 9 is the plane graph and the sectional drawing of the biosensor in the second embodiment of the present invention;
Figure 10 is the plane graph and the sectional drawing of the biosensor in the third embodiment of the present invention;
Figure 11 is the plane graph and the sectional drawing of the biosensor in the fourth embodiment of the present invention;
Figure 12 is the structural representation of the analytic unit of the biosensor in the first embodiment of the present invention;
Figure 13 is the perspective view of the insertion keeper in the fifth embodiment of the present invention;
Figure 14 is the plane graph and the sectional drawing of the biosensor in the fifth embodiment of the present invention;
Figure 15 is the decomposition diagram that the laseropuncture in the sixth embodiment of the present invention is used biosensor;
Figure 16 is total view that the laseropuncture in the sixth embodiment of the present invention is used biosensor;
Figure 17 be when the laseropuncture in the sixth embodiment of the present invention with biosensor at the sectional drawing of core when the longitudinal direction of Figure 16 dissects;
Figure 18 illustrates when the laseropuncture in the use sixth embodiment of the present invention is used biosensor the view of the relation between pick off, finger and the laser beam;
Figure 19 is when the laseropuncture in the use sixth embodiment of the present invention is used biosensor, shows the view of the situation in the laseropuncture process in detail;
Figure 20 is plane graph and the sectional drawing that the laseropuncture in the seventh embodiment of the present invention is used biosensor;
Figure 21 is plane graph and the sectional drawing that the laseropuncture in the seventh embodiment of the present invention is used biosensor;
Figure 22 illustrates the laseropuncture that how to use in the sixth embodiment of the present invention view of biosensor;
Figure 23 is the plane graph and the sectional drawing of the biosensor in the ninth embodiment of the present invention;
Figure 24 is the plane graph and the sectional drawing of another biosensor in the tenth embodiment of the present invention;
Figure 25 illustrates view how to recognize another slide plate in the ninth embodiment of the present invention;
Figure 26 is the plane graph and the sectional drawing of another biosensor in the 11st embodiment of the present invention;
Figure 27 is the perspective view of the laseropuncture device 1 of the 12nd embodiment of the present invention;
Figure 28 is the sketch map of puncture 507 of the laseropuncture device 1 of the 12nd embodiment of the present invention;
Figure 29 is the sketch map of puncture 514 of the laseropuncture device 1 of the 12nd embodiment of the present invention;
Figure 30 is the sketch map of laser irradiation condition of the laseropuncture device of the 13rd embodiment of the present invention;
Figure 31 is the zoomed-in view in hole of the puncture of the skin in the laser irradiation condition of laseropuncture device of the 13rd embodiment of the present invention;
Figure 32 is the sketch map of other laser irradiation condition of the laseropuncture device of the 13rd embodiment of the present invention;
Figure 33 is the sketch map of the laser oscillator 620 of lift-launch on the laseropuncture device of the 13rd embodiment of the present invention;
Figure 34 is the perspective view (1) of the laseropuncture device 1 of the 13rd embodiment of the present invention;
Figure 35 is the perspective view of the laseropuncture device 1 of the 14th embodiment of the present invention;
Figure 36 is the flow chart of operation of the laseropuncture device 1 of the 14th embodiment of the present invention;
Figure 37 is the view of the example of the periodic sound that shows that the laseropuncture device 1 of the 14th embodiment of the present invention uses;
Figure 38 is the block diagram of the laseropuncture device 1 of the 14th embodiment of the present invention;
Figure 39 is the sketch map of the puncture medicated cap 507 in the laseropuncture device 1 of the 15th embodiment of the present invention, and wherein the moisture measurement pick off is set in the puncture medicated cap 507;
Figure 40 is the sectional drawing that the puncture medicated cap 7 of the 15th embodiment of the present invention is used for the situation of skin;
Figure 41 is near the sectional drawing of the puncture medicated cap 507 of the 15th embodiment of the present invention;
Figure 42 is the view of use flow process of the laseropuncture device 1 of the 15th embodiment of the present invention;
Figure 43 is the sketch map of the situation about being corrected based on the moisture content of skin measurement result of the laser pulse beam energy in the embodiments of the invention;
Figure 44 is a moisture measurement sensor electrode 821, and the view of the example of the lip-deep humidity sensor 822 of setting laseropuncture device 1 in an embodiment of the present invention;
Figure 45 is the zoomed-in view of the laseropuncture point in the laseropuncture process in the embodiments of the invention;
Figure 46 is the sketch map of the insertion keeper in the 16th embodiment of the present invention;
Figure 47 is the sketch map of the insertion keeper in the 16th embodiment of the present invention;
Figure 48 is the sketch map of the biosensor in the 16th embodiment of the present invention;
Figure 49 is the sketch map of the insertion keeper among ground of the present invention 16 embodiment;
Figure 50 is the view of the oscillatory regime of the laser pulse bundle in the 17th embodiment of the present invention;
Figure 51 is the zoomed-in view of the postradiation laseropuncture point of the first laser pulse bundle in the 17th embodiment of the present invention, and the zoomed-in view (b) of the postradiation laseropuncture point of the second laser pulse bundle;
Figure 52 is the plane graph and the sectional drawing of the biosensor in the 18th embodiment of the present invention;
Figure 53 is the zoomed-in view (a) of the laseropuncture point in the laseropuncture process in the 18th embodiment of the present invention, and the zoomed-in view of the laseropuncture point behind the laseropuncture (b);
Figure 54 is the zoomed-in view of the laseropuncture point in the laseropuncture process of the 19th embodiment of the present invention;
Figure 55 is the laseropuncture schematic representation of apparatus in the 20th embodiment of the present invention;
Figure 56 is the flow chart of the operation of the laseropuncture device in the 20th embodiment of the present invention;
Figure 57 is the sketch map of the light wave shape in the 20th embodiment of the present invention;
Figure 58 is total view of traditional laser aid;
Figure 59 is the decomposition diagram of traditional biosensor;
Figure 60 is the flow chart of the operation of conventional laser sting device;
Figure 61 is the sketch map that is used to explain the skin of laseropuncture.
Reference marker
1: apparatus for measuring concentration of components
2,102: main body
3: display
4: the display switch button
5: the laser operations button
6: slide plate
7,207: insert keeper
8: biosensor
9: laser aid
10: battery
11: circuit
12,105: collecting lens
13: analyze Optical devices
14,213: cylinder
15,214: otch
16: projection
17,313: laser beam
18,107,314: finger
19: through hole
20: laseropuncture
21: plume
22,32: opening
31,51,100,251,301,451: substrate
33,54,63,65,254,304,325,454: film
52: filter
53,255,305,455: sample reagent
55,117,256,306,456: dividing plate
56,257,307,457: the sample reagent service duct
57,119,258,308,458: cover plate
58,259,459: airport
59: base openings
60: the dividing plate opening
61: the cover plate opening
62: the second openings
64: the three openings
66: photocell
67: light receiving element
101: the solid-state laser agitator
103: controller
104: lens cap
106: platform
108: the finger holder
110: introducing port
111: outlet
112,113: lead
114: measurement electrode
115: antielectrode
116: insulating barrier
118: spatial portion
218: the first electrodes
215: the first projections
216: the second projections
217: the three projections
425: switch
Preferred forms of the present invention
First embodiment
Hereinafter, biosensor in the first embodiment of the present invention and apparatus for measuring concentration of components will be described with reference to the accompanying drawings.Fig. 1 is the top view of the apparatus for measuring concentration of components in the first embodiment of the present invention, Fig. 2 is the internal structure sketch map of apparatus for measuring concentration of components when Fig. 1 observes from the side, and Fig. 3 is the lower interior portion structural representation when Fig. 1 apparatus for measuring concentration of components during from top observe.
In Fig. 1, apparatus for measuring concentration of components 1 comprises the display switch button 3 and the laser operations button 5 of display 1, display 3, and it is set in the main body 2; And comprise in addition, with respect to main body 2 interchangeable slide plates 6, be entrenched in insertion keeper 7 and biosensor 8 in the main body 2.Although Fig. 1 has shown that insertion keeper 7 and biosensor 8 are isolating, when insertion keeper 7 and biosensor 8 in operating process combine by chimeric.
In Fig. 2, it is the decomposition view of the internal structure when Fig. 1 observes from the side, and the reference marker identical with Fig. 1 represented the components identical with Fig. 1.Laser aid 9, rechargeable battery 10, circuit 11 and collecting lens 12 are arranged on main body 2 inside.In Fig. 3, it is for when the decomposition view of Fig. 1 from the internal structure in top when observation, and the reference marker identical with Fig. 1 and 2 represented the components identical with Fig. 1 and 2.The analysis of biosensor 8 is connected with circuit 11 by unshowned distribution with laser aid 9 with analysis Optical devices 13, laser operations button 5, display 3, display switch button 3, battery 4.Similarly, laser aid 9 is connected with battery 10 by unshowned distribution.
(insertion keeper)
Fig. 4 is a detailed perspective view of inserting keeper 7.In inserting keeper 7, otch 15 is arranged on an end of cylinder 14, and projection 16 is arranged on the cylindrical other end along three directions.
For cylinder 14, can use various plastic materials, as polyethylene, polrvinyl chloride, polypropylene, polystyrene, polyvinyl acetate, ABS resin, AS resin, acrylic resin, polyacetals, polyimide resin, Merlon, modification polyphenylene oxide (PPE), polybutyleneterephthalate (PPB), polyarylate, polysulfones, poly-inferior benzene sulfide, polyether-ether-ketone, fluororesin etc.In this embodiment, use the AS resin.The material that has low electrokinetic potential (zeta potential) on frosting is preferred.
The overall diameter that inserts keeper 7 is in such scope, and promptly biosensor 8 can be inserted into, and is preferably 3 to 25mm.In addition, the wall thickness of cylinder 14 is 0.2 to 3mm, and its length is 5 to 30mm.In this embodiment, inserting keeper forms cylindrical.Yet, also can use multiaspect body or similar.
(slide plate)
Fig. 5 is the detailed view of slide plate 6.Fig. 5 (a) is a plane graph, and Fig. 5 (b) is when the sectional drawing when core is cut open longitudinally.
(biosensor)
Fig. 6 is the detailed view of biosensor 8.Fig. 6 (a) is a plane graph, and Fig. 6 (b) is the sectional drawing when the center part is longitudinally dissectd, and Fig. 6 (c) is when the sectional drawing of center part during along transverse cross sectional.
And, has notch with the dividing plate 55 that forms the sample reagent service duct 56 of supplying sample reagent, the reagent layer 53 that is used for the filter 52 of separating particles composition and is impregnated with reagent, be clipped between cover plate 57 and the substrate 51, and this cover plate and substrate 51 integration settings.Reagent layer 53 forms by the reagent that coating comprises enzyme, electron acceptor, aminoacid, sugar alcohol, water soluble (CO) polymers etc.
The opening that penetrates substrate 51, dividing plate 55 and cover plate 57 respectively is respectively base openings 59, dividing plate opening 60 and cover plate opening 61.On the face relative that film 54 adheres to substrate 51, with covered substrate opening 59 with dividing plate 55.In addition, be provided with three second openings 62.Second opening 62 is provided with to such an extent that penetrate substrate 51, dividing plate 55 and cover plate 57 respectively, and some or all of being set in them is in alignment with each other.
The gross thickness of biosensor 8 is 0.1 to 1mm, and the length of minor face is 5 to 30mm, and the length of major axis is 30 to 60mm.
Then, will be described in detail operation.
(preparation)
At first, user is inserted into slide plate 6 in the main body 2.Then, similarly, insert keeper 7 by in the chimeric insertion body 2.Insert keeper 7 and be provided with otch 15, and otch 15 is arranged so that user can not make a mistake direction of insertion.Although used otch at this, cause and insert keeper 7 asymmetric other shapes, also can use as projection or a plurality of otch or projection.
Then, user inserts in the keeper 7 by chimeric biosensor 8 is inserted into.Second opening 62 of biosensor 8 is provided with to such an extent that they can be entrenched on the projection 16 of inserting keeper 7.Because second opening 62 is arranged in the biosensor 8 asymmetricly, user can insert this biosensor and can not make a mistake direction of insertion.With reference to figure 6, be provided with three second openings 62.Yet the present invention is not limited only to this, and the various settings of satisfying above-mentioned purpose can be used.The cross sectional shape of opening also can be a different shape, for example except the portions cut of two circles, also has circle, rectangle, square, polygon and ellipse.In the situation of the shape of second opening 62 that changes biosensor 8 and structure, need not, insert the also correspondingly change of shape, layout and quantity of the projection 16 of keeper 7.
Subsequently, user is attached to skin on the film 54 of biosensor 8, makes predetermined laseropuncture point can arrive base openings 59.Simultaneously, the projection 16 of inserting keeper 7 protrudes about 1 to 3mm from biosensor 8, and this projections press skin.This will stimulate predetermined laseropuncture point around.For this reason, this can alleviate the stimulation that user is felt during laseropuncture.
(laseropuncture)
After user is attached to biosensor 8 to skin, finish the preparation of laseropuncture thus, user is pressed laser operations button 5, to carry out laseropuncture.Laser aid 9 utilizes laser operations button 5 from the signal of circuit 11 generations and the electric energy oscillating laser bundle of battery 10.The laser beam of vibration is focused on by collecting lens 12, and the skin of puncture user.
Wavelength for laser beam, wavelength with high skin absorbs coefficient is preferred, and 3 mu m wavebands (the middle infrared semiconductor laser of optically pumped laser or er-doped medium), or 9 to 10 mu m wavebands (the middle infrared semiconductor laser of discharge excitation laser or carbon dioxide gas body medium) are operable.
In addition, the optically focused diameter of the laser beam on the skin is preferably below the φ 0.5mm, more preferably is below the φ 0.15mm.Although this optically focused shape is preferably circle, also can use major axis to be set at the following ellipse of 0.5mm.Laser beam is preferably with the pulse mode vibration, and the time width of the waveform of pulse is preferably more than the 1 μ s and below the 400 μ s.The irradiation of laser beam can be irradiation of pulse bundle or the irradiation of multiple-pulse bundle.
For collecting lens 12, by at calcium fluoride (CaF
2), carry out non-reflection on the substrate of yttrium-aluminium-garnet (YAG), germanium (Ge), zinc selenide (ZnSe), anhydrous synthetic quartz and the manufacturing of fluoride moulding material and apply (dielectric multilayer-film) and obtain collecting lens, and its focal length is preferably 5 to 30mm.In this embodiment, use the calcium fluoride substrate.In addition, collecting lens 12 is roughly circle, and its external dimensions is 6 to 30mm.
Fig. 7 is the zoomed-in view of the laseropuncture point in the laseropuncture process.Laser beam is focused on the finger 18 of the user that carries out laseropuncture by collecting lens 12, and its place is the place 17 of laser beam.The film 54 of the finger 18 contact biosensors 8 of user, and come the laser beam 17 of autonomous agent to propagate, its beam diameter little by little shrinks simultaneously, as Fig. 7.Laser beam 17 is set up, and passes the film 33, opening 32 of slide plate 6, the inside of inserting keeper 7, the opening 22 and the film 54 of biosensor 8 with propagation, on the finger 18 that focuses on user.
Constitute the opening 59,60 and 61 the opening diameter of the opening 32 of slide plate 6, the interior diameter that inserts the opening 22 of keeper 7 and biosensor 8 can be such diameter, this diameter makes laser beam 17 not cresteds basically, and all diameters needn't be identical.In addition, the minimum diameter of opening is 1mm.And with reference to figure 6, this opening is circular.Yet, may be different shape, for example rectangle, square, polygon and ellipse.
Fig. 8 is the zoomed-in view of the laseropuncture point behind laseropuncture and then.Because laseropuncture forms through hole 19 in film 54, and in the skin of the finger 18 of user, form laseropuncture hole 20, because the evaporation of skin histology generation plume 21
Be similar to Fig. 8, the plume 21 that produces when the tissue vaporisation of skin via the through hole 19 that laser beam irradiation film 54 forms, passes the opening 22 of biosensor 8, the inside of inserting keeper 7 and the opening of film 33 formation place.Because the through hole 19 of film 54 is very little, be similar to the laseropuncture hole 20 of skin, even lift his/her finger 18 at user from biosensor 8, the amount of the plume that flows back to is very little, therefore, user can avoid smelling the abnormal flavour of plume 21.
The apparatus for measuring concentration of components of this embodiment uses in glove box to carry out laseropuncture, wherein, is filled with nitrogen with the inside of the reliable gastight glove box in outside.Behind laseropuncture, when the glove box gas inside is extracted into the gas detecting tube inspection of assessment case and detected sulfide composition by air exhauster,, the level of detected quantity also equates even comparing with the situation of not carrying out laseropuncture.
For the base material of film 54, can use polyamide, polyester, polyimides, fluorine system, vinyl chloride, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.More preferably mulch film 54 has the absorptance higher to the wavelength of laser beam 17.Use Merlon in this embodiment.Because not only the absorptance of base material but also the absorptance of its thickness and additive work to this absorptance, preferably adjust thickness and additive, suitably to adjust this absorptance.
For the base material of film 33, can use polyamide, polyester, polyimides, fluorine system, vinyl chloride, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.Use cycloolefine polymer in this embodiment.More preferably mulch film 33 has for the lower absorptance of the wavelength of laser beam 17.Because not only the absorptance of base material but also the absorptance of its thickness and additive work to this absorptance, preferably adjust thickness and additive, suitably to adjust this absorptance.
The inside of film 54, film 33 and insertion keeper 7 also has the deodorization functions for the smell component of plume 21.The volatile material that is produced by the aminoacid of the protein component that constitutes skin histology is decomposed and flows in plume 21.Main target horny layer in the laseropuncture process is made up of the fibrous tissue of keratin protein, and fiber is by the cystine linkage combination, and this key is the key that comprises the sulfur part of more relatively amino acid cystine.These aminoacid decompose in evaporation process, produce volatile sulfur compounds or nitrogen compound, and the people feels as the sulphur compound of abnormal flavour.
Hydrogen sulfide or methyl mercaptan are mentioned as main sulphur compound.Hydrogen sulfide can pass through the chemosorbent deodorization, and can use manganese, copper and cobaltic composite oxygen compound.In addition, similarly, comprise by use oxide, hydroxide, composite oxygen compound or its mixture of manganese, copper, zinc and any one of cobalt can obtaining adsorbent for the effective chemical Absorption of hydrogen sulfide.
Chemosorbent is absorbing hydrogen sulphide chemically, finally is the form with sulfate or simple sulfur material.In addition, chemosorbent also has instrumentality, and its methyl mercaptan with same sulfenyl abnormal flavour is converted to the dimethyl disulfide with higher thresholds.Because this effect, the people can feel and is equivalent to the deodorant effect.Chemosorbent has the size of about 0.1 to 1 μ m, and the shape of this chemosorbent is not particularly limited.
And because dimethyl disulfide can be removed by the physical absorbent effect of the physical absorbent that carries, it can remove sulphur compound substantially.Hydrophobic zeolite with major part silicon can be used as physical absorbent.In addition, even use zeolite, meerschaum, Silicon stone, aluminium oxide etc., also can obtain identical effect.
These chemosorbents and physical absorbent can be by adding film 33 and 54 or insert keeper 7 base materials to, or by realizing the inside that is coated on film 33 and 54 or insert keeper 7.In addition, also preferably add or apply these adsorbents to/at substrate 51, dividing plate 55 or cover plate 57.
In addition, because the finger 18 of film 54 contact user preferably gives the film antibacterial characteristics.Various antibacterial can be used, and can by apply or mixing on base material/within realize.Antibacterial comprises Ag, Cu, Zn, Ni, Co or its alloy, TiO
2, ZnO, WO
3Deng.
In addition, antibacterial characteristics by on the film 54 that not only is implanted in biosensor 8 but also at biosensor 8, insert on the outer surface of keeper 7 and slide plate 6 and effectively.
In this embodiment, the wherein silver-colored antibacterial that is carried in the zirconium phosphate is added to coating material with 0.5wt%, is coated on the substrate then.And on the outer surface of substrate, the above-mentioned antibacterial of 0.5wt% is added in the resin that constitutes substrate.In addition, in these two processing methods, the antibacterial of 0.5wt% to 1.0wt% has antibacterial action, and is economical.
User from biosensor 8 move apart skin and push point of puncture around, with extrude 0.5 to several microliters of blood as the sample specimen.Subsequently, select the sample reagent service duct 56 of contact biosensor 8 one sides by making blood sampling, airport 58 actions, blood is collected in the biosensor 8 by capillarity thus.For blood, the blood cell composition in the blood is filtered device 52 on the way and captures, and only plasma fraction arrives sample reagent 53.
The chromogen of enzymic colorimetric well known by persons skilled in the art and enzyme are arranged in the sample reagent 53, this sample reagent 53 is in coating, adheres to or is printed on the substrate 51, or they by coating, printing, injection or kneading with the state of filter paper, film or other vector integration.The gravel size decision ground of sample reagent 53 is 3mm above square or rectangle on one side.
For the liquid to be analyzed that arrives sample reagent 53 is blood, if analyte is a glucose, then the reaction of glucose+oxygen+water-(glucoseoxidase) → gluconic acid+hydrogen peroxide occurs between the enzyme in glucose and the sample reagent 53, and the other reaction of hydrogen peroxide+chromogen-(peroxidase) → hydrogen peroxide+benzoquinonyl pigment occurs between product and the chromogen.By series reaction, sample reagent 53 shows the corresponding color of concentration with the analyte glucose.
Pyranose oxidase also can be used the replacement glucoseoxidase.Except peroxidase, also can use hemoglobin, Ferric sulfocyanate, ferriferro cyanide, potassium iodide, ammonium molybdate etc.
For color development (color development) situation, chromogen can have the absorbability in the visual range.Can use chromogen based on o-anisidine, benzidine, o-tolidine, tetramethyl benzidine and white; Can use by trinder base reagent, or derivatives thereof and anil etc. in conjunction with 4-amino-antipyrine and phenol acquisition.
Figure 12 is used to measure the structural representation of the analysis Optical devices 13 of sample reagent 53 color status.The light of the photocell 66 that is electrically connected with circuit 11 penetrates the opening that is arranged in the main body 2, and enter sample reagent 53 via the only transparent cover plate 57 for the photocell 66 of biosensor 8 basically, similarly, reflected light is by 67 detections of the light receiving element that is electrically connected with circuit 11.
Be used to prevent to enter from the dust of outside or analog, are openings that transparent window material also can be arranged on the analysis Optical devices 13 of main body 2 for photocell 66 wavelength of light emitted.As window material, can use Merlon, lucite, MS resin, polypropylene, ABS resin, polystyrene, epoxy resin, polyarete, polysulfones, poly--4-methylpentene-1, phenoxy resin, polyimide resin, alkene/maleimide copolymer, phenolic resin, the fire-resistant PC of non-bromo, cyclenes copolymer, methylpropanoic acid olefine resin, triacetyl cellulose and various glass material, for example silicon dioxide (SiO
2) and BK7.
Depend on the unicolor reflectance of visible light or near infrared light, or the such light intensity ratio of multi beam, the degree of colour developing is converted into concentration.As light source, can use combination, light emitting diode (LED) and the laser diode (LD) of lamp and frequency spectrum filter.In this embodiment, use glucoseoxidase, peroxidase and quinochrome, utilize wavelength to analyze for the reflected light of the light emitting diode of 610nm.
Be presented on the display 3 by the concentration of analyzing material to be measured in the blood that obtains, and discerned by user.Circuit 11 also comprises unshowned memory element, and analysis result was recorded together with measurement data and time or user code.Use display switch button 4, user can be discerned measurement result in the past afterwards once more.
As mentioned above, apparatus for measuring concentration of components according to the first embodiment of the present invention is provided with like this, its main body is provided with laser aid, collecting lens, be used for determining the Optical devices, circuit of the chromogenic reaction of biosensor, display and rechargeable battery that can the display analysis result, and slide plate and insertion keeper are inserted in the main body along the optical axis of laser beam, and biosensor is fitted in the insertion keeper.Therefore, because user does not smell abnormal flavour in the plume that produces in the laseropuncture process, and the reagent paper of the sampled point of contact user replaces when sampling at every turn, therefore do not worry to infect, and, can realize simple and easy use in a mechanism because user can sample and analyze.
In addition, the film that be penetrated by the laser beam on the laser beam optical axis is arranged in the biosensor, and this biosensor carries out component analysis by the chromogenic reaction of sample reagent.Therefore, the plume that produces in the laseropuncture process passes the hole discharge that penetrates from the skin of user, and the plume that produces in the laseropuncture process is discharged by penetrating from the skin of user.Therefore, plume does not leak into user, so user can be prevented from smelling the abnormal flavour in the plume that produces in the laseropuncture process.
And the film of the slide plate on the laser beam optical axis is provided with deodorization functions, similarly on the inner surface at least of the insertion keeper on the laser beam optical axis and the film that is arranged on the biosensor on the laser beam optical axis similarly be provided with deodorization functions.Therefore, can eliminate abnormal flavour in the plume that produces in the laseropuncture process.
Second embodiment
Hereinafter, biosensor according to a second embodiment of the present invention will be described in detail.In addition, only described with the first embodiment difference.
In first embodiment, the slide plate 6 of user use Fig. 5 is structurally different with the biosensor 8 of Fig. 6.In this embodiment, the biosensor 8 of Fig. 6 is as slide plate 8.The film 54 of the biosensor 8 of Fig. 6 and opening 59,60 and 61 are arranged on the optical axis of laser beam, with the film 33 and the opening 32 of the slide plate 6 of corresponding diagram 5.
During laseropuncture, because the film 54 that is used as the biosensor 8 of slide plate 6 is positioned at the side of collecting lens, and not on the focus of this collecting lens, therefore the laser beam energy density on the film is lower, and, can be used as biosensor again as the biosensor after the slide plate because laser beam does not form through hole.Correspondingly, because user can reduce the number of the type of the consumable goods that will manage, convenience is improved.
The 3rd embodiment
Hereinafter, the biosensor of the 3rd embodiment among the present invention will be described in detail.In addition, the point that is different from previous embodiment is only described.Biosensor is shown in Figure 10.Figure 10 (a) is a plane graph, and Figure 10 (b) is when the cross-sectional view when core dissects longitudinally, and Figure 10 (c) is when along the horizontal cross-sectional view when core dissects.When biosensor is used as slide plate, also be provided with the 3rd opening 64 and film 65.When biosensor when the slide plate, the 3rd opening 64 and film 65 are arranged on the optical axis of laser beam, even and film 65 be arranged so that laser beam repeatedly is radiated on this film and also can on this film, do not form through hole.
For film 65, can use various plastics or resin, for example polyamide, polyester, polyimides, fluorine system, vinyl chloride, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.In addition, also can use by at calcium fluoride (CaF
2), carry out that non-reflection applies (dielectric multilayer-film) on yttrium-aluminium-garnet (YAG), germanium (Ge), zinc selenide (ZnSe), anhydrous synthetic quartz and the fluoride moulding material and the film that obtains.Use cycloolefine polymer in this embodiment.
More preferably, film 65 has for the lower absorptance of the wavelength of laser beam.Because not only the absorptance of base material but also the absorptance of its thickness and additive work to this absorptance, preferably adjust thickness and additive, suitably to adjust this absorptance.In addition, be similar to first embodiment, preferably give film 65 with deodorization functions and antibacterial characteristics.
Correspondingly, buying 25 to 50 pieces of biosensors usually only can use one of them as slide plate as the user of a unit.Therefore, prevented decline, and do not needed to carry out the replacement of slide plate at every turn owing to the analytical performance that exposes the sample reagent that biosensor and air long period cause.Therefore, can improve the convenience of user.
The 4th embodiment
Hereinafter, Fig. 9 and 11 shows the biosensor in the fourth embodiment of the invention.Fig. 9 illustrates the biosensor that obtains by biosensor among first embodiment that changes Fig. 6.Fig. 9 (a) is a plane graph, and Fig. 9 (b) is when the cross-sectional view when core dissects longitudinally, and Fig. 9 (c) is when along the horizontal cross-sectional view when core dissects.In addition, Figure 11 has shown the biosensor that obtains by biosensor among the 3rd embodiment that changes Figure 10.Figure 11 (a) is a plane graph, and Figure 11 (b) is when the cross-sectional view when core dissects longitudinally, and Figure 11 (c) is when along the horizontal cross-sectional view when core dissects.Hereinafter, the point that is different from previous embodiment is only described.
In the biosensor of Fig. 9 and 11, except film 54 is arranged on a side of opening 59,60 and 61, film 63 also is arranged on the opposite side of these openings.For film 63, can use various plastics or resin, for example polyamide, polyester, polyimides, fluorine system, vinyl chloride, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.In addition, also can use by at calcium fluoride (CaF
2), carry out that non-reflection applies (dielectric multilayer-film) on yttrium-aluminium-garnet (YAG), germanium (Ge), zinc selenide (ZnSe), anhydrous synthetic quartz and the fluoride moulding material and the film that obtains.Use cycloolefine polymer in this embodiment.More preferably, film 63 has for the lower absorptance of the wavelength of laser beam.Because not only the absorptance of base material but also the absorptance of its thickness and additive work to this absorptance, preferably adjust thickness and additive, suitably to adjust this absorptance.In addition, be similar to first embodiment, preferably give film 63 with deodorization functions and antibacterial characteristics.
In first embodiment, the plume that comprises smell component that in the laseropuncture process, produces, via the through hole in the film 54 that in the laseropuncture process, is formed on the people side, flow to by the opening 59,60 and 61 of biosensor 8, the inside of inserting keeper 7 and the space that slide plate 6 forms.By these deodorization functions, prevent that user from smelling the abnormal flavour of plume.
In this embodiment, the plume that comprises smell component that in the laseropuncture process, produces, via the through hole in the film 54 that in the laseropuncture process, is formed on biosensor 8, flow to opening 59,60 and 61 by biosensor 8, and the space that in the laseropuncture process, does not form film 63 formation of through hole therein.The deodorization composition of smell component tunicle 54 and film 63 is eliminated, and prevent that thus user from smelling the abnormal flavour of plume, so convenience is improved.
In addition, for sample specimen, can use for example blood or interstitial fluid, and, can use blood glucose levels (concentration of glucose) or various serum or biochemical project for analyte via the skin sampling.
The 5th embodiment
Hereinafter, will describe the fifth embodiment of the present invention in detail.Figure 13 is a detailed perspective view of inserting keeper 207.In Figure 13, reference marker 213 expression cylinders, first projection of one end of the otch of one end of reference marker 214 expression cylinders 213, reference marker 215 expression cylinders 213, second projection of one end of reference marker 216 expression cylinders 213, the 3rd projection of one end of reference marker 217 expression cylinders 213, reference marker 218 are illustrated in first electrode that extends towards first projection 215 on the outer wall of cylinder 213.In addition, although not shown, the outer wall of cylinder 213 is provided with second electrode that extends towards second projection 216.
For cylinder 213, can use various plastics, they are polyethylene, polrvinyl chloride, polypropylene, polystyrene, polyvinyl acetate, ABS resin, AS resin, acrylic resin, polyacetals, polyimide resin, Merlon, modification polyphenylene oxide (PPE), polybutyleneterephthalate (PPB), polyarylate, polysulfones, poly-inferior benzene sulfide, polyether-ether-ketone, fluororesin etc.Use the AS resin in this embodiment.The material that has low electrokinetic potential on frosting is preferred.First electrode 218 and second electrode can be made by the material with electric conductivity.Use copper in this embodiment.Preferably, can use metallic alloy, for example copper and aluminum, and wherein metallic alloy is electroplated through metal and the material of antirust processing.
The overall diameter that inserts keeper 207 is in such scope, and promptly biosensor 8 can be inserted into, and is preferably 3 to 25mm.In addition, the wall thickness of cylinder 213 is 0.2 to 3mm, and its length is 5 to 30mm.In this embodiment, inserting keeper forms cylindrical.Yet, also can use multiaspect body or similar.
(biosensor)
Figure 14 is the detailed view according to the biosensor 8 of this embodiment.Figure 14 (a) is a plane graph, and Figure 14 (b) is when the cross-sectional view when core dissects longitudinally, and Figure 14 (c) is when along the horizontal cross-sectional view when core dissects.
Labelling 258 expression insulation cover plates, the airport of reference marker 259 expression circular.The material of cover plate 258 is preferably plastic foil, and comprises polyester, polyolefin, polyamide, polyethers, polyamidoimide, polystyrene, Merlon, polyethylene-ρ-phenyl sulfur, polrvinyl chloride etc.Use Merlon in this embodiment.In addition, copolymer, composite material and their bridge material can be used for cover plate 258, but and the cover plate of used thickness 0.01mm to 0.5mm.
And dividing plate 256 has notch portion to form the sample reagent service duct 257 of supply sample reagent, and reagent layer 255 is impregnated with reagent, and this dividing plate 256 and reagent layer 255 are clipped between cover plate 258 and the substrate 251, and this cover plate and substrate 251 integration settings.
Dividing plate 256 is set to electrode galvanic couple 253 and the measurement electrode 252 on the covered substrate 251; The intermediary rectangular cutout of the leading edge of sample service duct 257 by being arranged on dividing plate 256 partly forms; And reagent layer 255 forms by applying the reagent that comprises enzyme, electron acceptor, aminoacid, sugar alcohol, water soluble (CO) polymers etc. on the electrode galvanic couple 253 that exposes in dividing plate 256 notch portion and the measurement electrode 252.
And base openings 260, dividing plate opening 261 and cover plate opening 262 are for penetrating the opening of substrate 251, dividing plate 256 and cover plate 258 respectively.And film 254 adheres to substrate 251 and dividing plate 256 facing surfaces, with covered substrate opening 260.The gross thickness of biosensor is 0.1 to 1mm, and the length of its minor face is 5 to 30mm, and the length of its major axis is 30 to 60mm.
In addition, be provided with second opening 263 and the 3rd opening 264, and measurement electrode 252 and electrode galvanic couple 253 are exposed to the part in the opening 264.Second opening 263 and the 3rd opening 264 are set to pass respectively substrate 251, dividing plate 256 and cover plate 258, and these openings partly or entirely be set to be in alignment with each other.
Then, will be described in detail operation.
(preparation)
At first, user is inserted into slide plate 6 in the main body 2, then, similarly, inserts keeper 207 by in the chimeric insertion body 2.Insert keeper 207 and be provided with otch 214, and this otch 214 is arranged so that user can not make a mistake direction of insertion.Although used otch, insert keeper 207 asymmetric other shapes but also can use to cause, as projection or a plurality of otch or projection at this.
Then, user inserts in the keeper 207 by chimeric biosensor 8 is inserted into.Second opening 263 of biosensor 8 and the 3rd opening 264 are arranged so that they can be entrenched on first projection 215, second projection 216 and the 3rd projection 217 of inserting keeper 207.Because second opening 263 is to be arranged in the biosensor 8 asymmetricly, user can insert this biosensor and can not make a mistake direction of insertion.With reference to Figure 14, be provided with one second opening 263.Yet the present invention is not limited only to this, and the various settings of satisfying above-mentioned purpose can be used.The cross sectional shape of the 3rd opening 264 and second opening 263 can also be a different shape, for example except the part stripping and slicing of two circles, also has circle, rectangle, square, polygon and ellipse.In the situation of the shape of second opening 263 that changes biosensor 8 and the 3rd opening 264 and structure, need not, shape, structure and the quantity of inserting the projection that comprises first projection 215, second projection 216 and the 3rd projection 217 of keeper 207 also correspondingly change.
When biosensor is inserted into when inserting in the keeper 207 by chimeric, electrode galvanic couple 253 in the 3rd opening 262 of biosensor 8 and measurement electrode 252 are electrically connected respectively with the second electrode (not shown) and first electrode 218 of the periphery of the cylinder 213 of insertion keeper 207.And, although it is not shown in Fig. 1 to 3, the terminal that is used for first electrode 218 and second electrode is arranged on the sidepiece of main body 2 sides, and pass through chimeric first electrode 218 that is inserted into main body 2 and second electrode that make to insert keeper 207 are electrically connected with circuit 11 in the main body 2.If biosensor 8 and main body 2 place the state of mutual electrical connection, apparatus for measuring concentration of components 1 will be in the readiness from the laseropuncture and the measuring concentration of components of circuit 11, and the fact that this device has reached this state is presented on the display 3, makes the user can recognize this fact thus.
Subsequently, user is attached to skin on the film 54 of biosensor 8, makes predetermined laseropuncture point can arrive base openings 260.At this moment, insert the projection of keeper 207, that is, first projection 215, second projection 216 and the 3rd projection 217 protrude about 1 to 3mm from biosensor 8, and this projections press skin.This will stimulate predetermined laseropuncture point around.For this reason, this can alleviate the stimulation that user is felt during laseropuncture.
(laseropuncture)
After user is attached to biosensor 8 to skin, finish the preparation of laseropuncture thus, user is pressed laser operations button 5 to carry out laseropuncture.Laser aid 9 utilizes laser operations button 5 from the signal of circuit 11 and the electric energy oscillating laser bundle of battery 10.The laser beam of vibration is focused on by collecting lens 12, and the skin of puncture user.
Wavelength for laser beam, wavelength with high skin absorbs coefficient is preferred, and 3 mu m wavebands (the middle infrared semiconductor laser of optically pumped laser or er-doped medium), or 9 to 10 mu m wavebands (the middle infrared semiconductor laser of discharge excitation laser or carbon dioxide gas body medium) are operable.In addition, the optically focused diameter of the laser beam on the skin is preferably below the φ 0.5mm, and more preferably is below the φ 0.15mm.Although this optically focused shape is preferably circle, also can use its major axis to be set at the following ellipse of 0.5mm.Laser beam preferably vibrates in the mode of pulse, and the time width of the waveform of pulse is preferably more than the 1 μ s and below the 400 μ s.The irradiation of laser beam can be the irradiation of pulse bundle or the irradiation of multiple-pulse bundle.
For collecting lens 12, by at calcium fluoride (CaF
2), to carry out that non-reflection applies (dielectric multilayer-film) and obtain collecting lens on the substrate made of yttrium-aluminium-garnet (YAG), germanium (Ge), zinc selenide (ZnSe), anhydrous synthetic quartz and fluoride moulding material be operable, and its focal length is preferably 5 to 30mm.Use the calcium fluoride substrate in this embodiment.In addition, collecting lens 12 is roughly circle, and its overall diameter is 6 to 30mm.
Behind laseropuncture, user from biosensor 8 move apart skin and push point of puncture around, with extrude 0.5 to several microliters of blood as the sample specimen.Therefore, select the sample reagent service duct 56 of a side of contact biosensor 8 by making blood sampling, airport 259 actions, blood is collected in the biosensor 8 by capillarity thus.For the blood of sampling, the material to be measured in the blood of reagent and sampling is reaction each other in reagent layer 255.For the electric charge that produces in the reaction, analyze the quantity of electric charge by the electrode of measurement electrode 252, electrode galvanic couple 253 and insertion keeper 207 by circuit 11.Be presented on the display 3 by the concentration of analyzing the material to be measured in the blood that obtains, and discerned by user.Circuit 11 also comprises unshowned memory element, and analysis result was recorded together with measurement data and time or user code.Use display switch button 4, user can be discerned measurement result in the past afterwards once more.
Apparatus for measuring concentration of components according to this embodiment of the invention, main body is provided with laser aid, collecting lens, can analyzes the circuit of composition, can the display analysis result display and rechargeable battery, and slide plate and insertion keeper are inserted into along in the main body of the optical axis of laser beam, and biosensor is entrenched in the insertion keeper.Therefore, because user does not smell abnormal flavour in the plume that produces in the laseropuncture process, and the reagent paper of the sampled point of contact user replaces when sampling at every turn, therefore do not worry to infect, and, can realize simple and easy use in a mechanism because user can sample and analyze.
In addition, will be arranged in the biosensor on the laser beam optical axis by the film that laser beam penetrates.Therefore, the plume that produces in the laseropuncture process passes the puncturing hole discharge from the skin of user, and the plume that produces in the laseropuncture process is discharged from the skin of user by this puncture.Therefore, plume does not leak into user, so user can be prevented from smelling the abnormal flavour in the plume that produces in the laseropuncture process.
And by on the sidepiece that is inserted into main intravital insertion keeper dissymmetrical structure being set, user can not made a mistake direction of insertion, and therefore can realize simple and easy use.
And, by a structure is set, biosensor and to insert chimeric between the keeper be chimeric realization between opening by biosensor and the projection of inserting keeper wherein, user can not made a mistake the cooperation direction, and therefore can realize simple and easy use.
And, by a structure is set, biosensor and to insert chimeric between the keeper be chimeric realization between opening by biosensor and the projection of inserting keeper wherein, user can not made a mistake the cooperation direction, and can skid off hardly.Therefore can realize simple and easy use.
And, by a structure is set, wherein the measurement electrode of biosensor and electrode galvanic couple by biosensor and insert chimeric between the keeper and be arranged on lip-deep each electrode that inserts keeper and be electrically connected.Thus, in the component analysis process of the body fluid of sampling by laseropuncture, user can carry out component analysis, and need not remove reagent paper and reinstall it for component analysis once more, and can realize simple and easy use thus.
And deodorization functions is arranged on the film of the slide plate on the optical axis of laser beam, at least on the inner surface of the insertion keeper on the optical axis at laser beam similarly and similarly on the film of the biosensor on the optical axis at laser beam.Therefore, can eliminate abnormal flavour in the plume that produces in the laseropuncture process.
And, by on biosensor, insertion keeper and slide plate, antibacterial functions being set, can prevent that user is infected in use.
In addition, for the sample specimen, can use these for example blood and interstitial fluids, and, can use blood glucose levels (concentration of glucose) for analyte via the sampling of skin, or various serum or biochemical project.
In addition, be used as in the situation of analyte at blood glucose levels (concentration of glucose), as can be as the example of reagent, the enzyme combination of coenzyme and medium (mediator) in some cases comprising: glucoseoxidase+ferricyanic acid potassium or ferricynium; And glucoseoxidase+pyrroloquinolinequinine or nicotinamide adenine+phenanthroline quinine, osmium comple or potassium ferricyanide.
The 6th embodiment
The laseropuncture of the sixth embodiment of the present invention is presented among Figure 15 to 17 with biosensor.Figure 15 is the decomposition diagram of biosensor, and Figure 16 is total view of biosensor, and Figure 17 is when the pick off of Figure 16 cross-sectional view when core dissects longitudinally.
Use Merlon in this embodiment.
In addition, copolymer, composite material and bridge material can be used for cover plate 308, but and the cover plate of used thickness 0.01mm to 0.5mm.
And, have the dividing plate 306 of otch with the sample reagent service duct 307 of formation supply sample reagent, and the reagent layer 305 that is impregnated with reagent, be clipped between cover plate 308 and the substrate 301, and this cover plate and substrate 301 integration settings.
Dividing plate 306 is set to electrode galvanic couple 303 and the measurement electrode 302 on the covered substrate 301, the intermediary rectangular cutout of the leading edge of sample service duct 307 by being arranged on dividing plate 306 partly forms, and reagent layer 305 forms by applying the reagent that comprises enzyme, electron acceptor, aminoacid, sugar alcohol, water soluble (CO) polymers etc. on the electrode galvanic couple 303 that exposes in dividing plate 306 notch portion and the measurement electrode 302.
And base openings 310, dividing plate opening 311 and cover plate opening 312 are the openings that penetrate substrate 301, dividing plate 306 and cover plate 308 respectively.And film 304 adheres on substrate 301 and dividing plate 306 facing surfaces, with covered substrate opening 310.In addition, film 325 adhere to cover plate 308 with dividing plate 306 facing surfaces on, to cover cover plate opening 312.The gross thickness of biosensor is 0.1 to 1mm, and the length of its minor face is 5 to 30mm, and the length of its major axis is 30 to 60mm.
Then, will be described in detail operation.
Figure 22 explains the view how to use.In Figure 22, reference marker 319 expression main bodys, the display device on the reference marker 321 expression main bodys 319, the operating means on the reference marker 322 expression main bodys 319, and reference marker 320 expression laseropunctures are with biosensor (hereinafter being sometimes referred to as pick off).In main body 319 inside, be provided with unshowned laser oscillator, unshowned gathering is from the beam condensing unit of the laser beam of laser oscillator, the unshowned circuit board that is used to operate and control laser oscillator similarly similarly.
For the wavelength of laser beam, the wavelength with high skin absorbs coefficient is preferred, and 3 mu m wavebands or 9 to 10 mu m wavebands are operable.In addition, the optically focused diameter of the laser beam on the skin is preferably below the φ 0.5mm, and more preferably is below the φ 0.15mm.Although this optically focused shape is preferably circle, also can use its major axis to be set at the following ellipse of 0.5mm.Laser beam preferably vibrates in the mode of pulse, and the time width of the waveform of pulse is preferably more than the 1 μ s and below the 400 μ s.The irradiation of laser beam can be the irradiation of pulse bundle or the irradiation of multiple-pulse bundle.
And although not shown, main body 319 keeps the laseropuncture biosensor.Be provided with in main body: apply constant voltage to the change-over circuit of laseropuncture with biosensor, will be voltage its this moment with the corresponding current conversion of concentration of analyte in the sample specimen; The A-D transducer is a digital numerical value with the voltage transitions of being changed; Memorizer, the numerical value that its record is measured; And circuit board, be used for operation and control these elements.In addition, although not shown, be provided with power supply to the said elements power supply.
Measurement numerical value, the state that is stored in the interior numerical value of memorizer, device, date and time etc. are presented in the display device 321.Operating means 322 is buttons for example, and carry out oscillating operation, the measurement of laser beam start-up operation, show the switching manipulation of display device 321 etc.
User is provided with pick off 320, makes pick off can contact the whole surface of main body 319.User is placed on his/her finger on the pick off 320, and therefore predetermined point of puncture can arrive the almost center of the film 304 of pick off 320.With from the laser oscillator oscillating laser, on the skin of finger, be used for the puncture of blood sampling by operating operation device 322.After puncture, user at first separates his/her finger from pick off 320, and the extruding point of puncture near, with extrude as the sample specimen 0.5 to several microliters of blood.Subsequently, the sample reagent service duct 307 of the side by making blood sampling whisker sensor 320, blood is collected in the pick off 320.Analysis result is presented in the display device 321, and is discerned by user.
Figure 18 is the view of the relation between pick off, finger and the laser beam that shows when using.In Figure 18, carry out the finger 314 of user of laseropuncture and the place of the laser beam 313 that focused on by beam condensing unit and be illustrated.The film 304 of finger 314 touch sensors 320 of user, and laser beam 313 propagates simultaneously from main body that its beam diameter little by little shrinks, as Figure 18.Laser beam 313 is set up, and passes cover plate opening 312, dividing plate opening 311 and the base openings 310 of pick off 320 with propagation, and focuses on the finger 314 of user.
Opening 310,311 and 312 opening diameter can be such diameters, and promptly laser beam 313 does not have crested basically, and all diameters needn't be identical.In addition, the minimum diameter of opening is 1mm.And with reference to Figure 15 to 17, these openings are circular.Yet, can be for example rectangle, square, polygon and oval-shaped different shape.
Figure 19 is the view that shows the situation in the laseropuncture process in detail.In Figure 19, laser beam forms through hole 315 in film 304.Similarly, the hole 316 of laseropuncture is formed on the skin of finger 314 of user.In the laseropuncture process, when skin histology evaporates, produce plume 317.
As Figure 19, the plume 317 that produces during the skin histology evaporation via the through hole 315 that forms by laser beam irradiation film 304, flows to the opening of base openings 310, dividing plate opening 311, cover plate opening 313 and film 325 formation place.Because the through hole 315 of film 304 is similar to the hole 315 of laseropuncture of skin and very little, even after user lifted his/her finger 314 from pick off, the amount of the plume that flows back to was also very little, so user can avoid smelling the abnormal flavour of plume 317.
For the base material of film 304, can use nylon, polyester, polyimides, fluorine system, vinyl chloride, polystyrene, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.Use polyethylene in this embodiment.
More preferably mulch film 304 has the absorptance higher to the wavelength of laser beam 313.Because not only the absorptance of base material but also the absorptance of its thickness and additive work to this absorptance, preferably adjust thickness and additive, suitably to adjust this absorptance.
For the base material of film 325, can use nylon, polyester, polyimides, fluorine system, vinyl chloride, polystyrene, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.Use polystyrene in this embodiment.
More preferably mulch film 325 has for the lower absorptance of the wavelength of laser beam 313.Because not only the absorptance of base material but also the absorptance of its thickness and additive work to this absorptance, preferably adjust thickness and additive, suitably to adjust this absorptance.
Hydrogen sulfide or methyl mercaptan are mentioned as main sulphur compound.Hydrogen sulfide can pass through the chemosorbent deodorization, and can use manganese, copper and cobaltic composite oxygen compound.In addition, similarly, comprise any one oxide, hydroxide, composite oxygen compound or its mixture of manganese, copper, zinc and cobalt, can obtain adsorbent for the effective chemical Absorption of hydrogen sulfide by use.
Chemosorbent is absorbing hydrogen sulphide chemically, finally is the form with sulfate or simple sulfur material.In addition, chemosorbent also has instrumentality, and the methyl mercaptan of the sulfenyl abnormal flavour that its conversion is same is the dimethyl disulfide with higher thresholds.Because this effect, the people can feel and is equivalent to the deodorant effect.Chemosorbent has the size of about 0.1 to 1 μ m, and the shape of this chemosorbent is not particularly limited.
And because dimethyl disulfide can be removed by the physical absorbent effect of using the physical absorbent that loads, it can remove sulphur compound substantially.Hydrophobic zeolite with major part silicon can be used as physical absorbent.In addition, even use zeolite, meerschaum, Silicon stone, aluminium oxide etc., also can obtain identical effect.
These chemosorbents and physical absorbent can be by in the base materials that adds film to, or realize by being coated on the film.In addition, also preferably add or apply these adsorbents on substrate or its.
In addition, because the finger 314 of film 304 contact user preferably gives this film antibacterial characteristics.Various antibacterial can be used, and can by apply or mixing on base material/within realize.These antibacterial comprise Ag, Cu, Zn, Ni, Co or its alloy, TiO
2, ZnO, WO
3Deng.In this embodiment, the wherein silver-colored antibacterial that is carried in the zirconium phosphate is added in the film 304 with 0.5wt%.
Laseropuncture biosensor according to a sixth embodiment of the invention, and film is arranged in the pick off, and in the one side, and give these film deodorization functions.Therefore, comprise that the plume of the abnormal flavour that produces in the laseropuncture process can be prevented from leaking into the outside, and can eliminate smell component.
The 7th embodiment
Next, Figure 20 has shown the laseropuncture biosensor of the seventh embodiment of the present invention.Figure 20 (a) is a plane graph, and Figure 20 (b) is when the vertically cross-sectional view when core dissects along Figure 20 (a).The 7th embodiment is different from the 6th embodiment part and only is newly to be provided with three second openings 323.Second opening 323 is set to pass respectively substrate 301, dividing plate 306 and cover plate 308, and in these openings some or all are set to be in alignment with each other.
The operation of second opening 323 is as follows.In the 6th embodiment, pick off links together by contacting with main body.Yet in the 7th embodiment, projection is arranged in the main body to align with second opening 323.User can insert projection to pick off, thus it is connected with main body.For this reason, the relative distance between laser oscillator and the pick off can be determined, and the shape in the hole of laseropuncture can stably obtain.
In addition, second opening 323 is arranged on asymmetric position along the minor face of pick off.Therefore, user can insert biosensor and not mistaken direction of insertion.And, when user is attached to pick off to his/her finger, the projection from second opening, 323 projectioies of main body will stimulate predetermined laseropuncture point near.Therefore, can alleviate user and during laseropuncture, feel stimulation.
In addition, with reference to Figure 20, be provided with three second openings 323.Yet, the invention is not restricted to this, can use the various settings of satisfying above-mentioned purpose.The cross sectional shape of second opening 323 also can be a different shape, and for example circle, rectangle, square, polygon and ellipse are except the part stripping and slicing of Double Circle.
As mentioned above, laseropuncture biosensor according to a seventh embodiment of the invention, new opening are provided with to such an extent that leave and be filtered the opening that device covers.Thus, the relative distance between laser oscillator and the pick off can be determined, and the shape in the hole of laseropuncture can stably obtain.And the closure between pick off and the main body can accurately be provided with, and can alleviate the stimulation during the laseropuncture, and this depends on the stimulation that causes from the projection of opening projection.
The 8th embodiment
Then, the laseropuncture of the eighth embodiment of the present invention is illustrated among Figure 21 with biosensor.Figure 21 (a) is a plane graph, and Figure 21 (b) is when the vertically cross-sectional view when core dissects along Figure 21 (a), and Figure 21 (c) is when the laterally cross-sectional view when core dissects along Figure 21 (a).
The 8th embodiment is different from the 6th embodiment part and is newly to be provided with second opening 323 and the 3rd opening 324, and the measurement electrode 302 and the electrode galvanic couple 303 that are exposed to the surface are moved in the opening 324, be different from the 7th embodiment part and be that two of second opening 323 become the 3rd opening 324, the measurement electrode 302 and the electrode galvanic couple 303 that are exposed to the surface are arranged so that and are exposed to respectively in the 3rd opening 324.Second opening 323 and the 3rd opening 324 are set to pass respectively substrate 301, dividing plate 306 and cover plate 308, and in these openings some or all are set to be in alignment with each other.
The operation of second opening 323 and the 3rd opening 324 is as follows.In the 6th embodiment, pick off links together by contacting with main body.Yet in the 8th embodiment, projection is arranged in the main body, to align with second opening 323 and the 3rd opening 324.They with projection that the 3rd opening 324 aligns in, the electrode that is used for the analyte concentration of electroanalysis sample specimen extends in main body.Therefore, user can insert projection to pick off, thus it is electrically connected and mechanical connection with main body.Thus, the relative distance between laser oscillator and the pick off can be determined, and the shape in the hole of laseropuncture can stably obtain.And insertion can be by electro-detection, and user can be discerned and insert mistake.Therefore, the convenience of user is improved.
In addition, second opening 323 and the 3rd opening 324 are arranged in the asymmetric position along the short side direction of pick off.Therefore, user can insert biosensor and not mistaken direction of insertion.And, when user is attached to pick off to his/her finger, the projection from second opening 323 and the 3rd opening 324 projectioies of main body will stimulate predetermined laseropuncture point near.Therefore, can alleviate user and during laseropuncture, feel stimulation.
In addition, with reference to Figure 21, in three points second opening 323 and the 3rd opening 324 are being set altogether.Yet, the invention is not restricted to this, can use the various settings of satisfying above-mentioned purpose.The cross sectional shape of second opening 323 and the 3rd opening 324 also can be a different shape, and for example circle, rectangle, square, polygon and ellipse are except the part stripping and slicing of Double Circle.
As mentioned above, according to the laseropuncture biosensor of the eighth embodiment of the present invention, new opening is provided with to such an extent that leave the opening that is filtered the device covering, and the direction electrodes exposed is in these openings.Thus, the relative distance between laser oscillator and the pick off can be determined, and the shape in the hole of laseropuncture can stably obtain.And because the state that inserts can be by electro-detection and affirmation, user can be discerned the insertion mistake.And the closure between pick off and the main body can accurately be provided with, and can alleviate the stimulation during the laseropuncture, and this depends on the stimulation that causes from the projection of opening projection.
In addition, for the sample specimen, can use these for example blood and interstitial fluids, and, can use blood glucose levels (concentration of glucose) for analyte via the sampling of skin, or various serum or biochemical project.
In addition, as in the situation of analyte, as can be as the example of reagent, the enzyme combination of coenzyme and medium in some cases comprises: glucoseoxidase+ferricyanic acid potassium or ferricynium at blood glucose levels (concentration of glucose); And glucoseoxidase+pyrroloquinoline quinine or nicotinamide adenine+phenanthroline quinine, osmium comple or potassiumferricyanide.
The 9th embodiment
Figure 23 is the detailed view according to the biosensor 8 of this embodiment.Figure 23 (a) is a plane graph, and Figure 23 (b) is when the cross-sectional view when core dissects longitudinally, and Figure 23 (c) is when along the horizontal cross-sectional view when core dissects.
On the surface of substrate 451, polyethylene terephthalate, polyester, polyolefin, polyamide, polyethers, polyamidoimide, polystyrene, Merlon, polyethylene-ρ-phenyl sulfur, polrvinyl chloride etc. have wherein been used, use polyethylene terephthaldehyde base acid esters in this embodiment, form by silk screen printing or sputter vapour deposition process by for example electrode galvanic couple 453 and measurement electrode 452 golden or palladic noble metal or for example conductive material manufacturing of carbon.
And, have notch portion with dividing plate 456 that forms the sample reagent service duct 457 of supplying sample reagent and the reagent layer 455 that is impregnated with reagent, be clipped between cover plate 458 and the substrate 451, and cover plate and substrate 451 integration settings.
Dividing plate 456 is set to electrode galvanic couple 453 and the measurement electrode 452 on the covered substrate 451, the intermediary rectangular cutout of the leading edge of sample service duct 457 by being arranged on dividing plate 456 partly forms, and reagent layer 455 forms by applying the reagent that comprises enzyme, electron acceptor, aminoacid, sugar alcohol, water soluble (CO) polymers etc. on the electrode galvanic couple 453 that exposes in the notch portion of dividing plate 456 and the measurement electrode 452.
And base openings 460, dividing plate opening 461 and cover plate opening 462 are for penetrating the opening of substrate 451, dividing plate 456 and cover plate 458 respectively.And film 454 adheres on substrate 451 and dividing plate 456 facing surfaces, with covered substrate opening 460.The gross thickness of biosensor is 0.1 to 1mm, and the length of its minor face is 5 to 30mm, and the length of its major axis is 30 to 60mm.
In addition, be provided with second opening 463 and the 3rd opening 464, measurement electrode 452 and electrode galvanic couple 453 are exposed to the part in the opening 464.Second opening 463 and the 3rd opening 464 are set to pass respectively substrate 451, dividing plate 456 and cover plate 458, and in these openings some or all are set to be in alignment with each other.
Then, will be described in detail operation.
(installation of slide plate)
At first, user is inserted into slide plate 6 in the main body 2.In the present invention, biosensor 8 can be used as slide plate 6.The film 454 and the opening 460,461 and 462 of the biosensor 23 by Figure 23 have represented and have prevented that because the function of the pollution (bluring) of the collecting lens 12 that the evaporant in the laseropuncture process causes, this is the purpose of slide plate 6.Therefore, because user does not need and prepares slide plate 6 dividually for the requisite biosensor 8 of component analysis, the convenience of user is improved.
Figure 25 is used to allow main body 2 to discern the schematic structure chart of the installation of slide plates 6.Be provided with the switch 425 that is electrically connected with circuit 11 in the main body 2, and switch 425 is opened (Figure 25 (a)) when slide plate 6 does not insert.In the time of in slide plate 6 is inserted into main body 2, switch 425 enters closed condition, and subsequently, circuit 11 can be discerned the insertion (Figure 25 (b)) of slide plate 6.
The tenth embodiment
Now, the method for the installation of identification slide plate 6 is to use the method for the electrode on the slide plate 6.Figure 24 has shown by revising the biosensor that the slide plate identical with the biosensor of Figure 23 6 obtains.Figure 24 (a) is a plane graph, and Figure 24 (b) is when the cross-sectional view when core dissects longitudinally, and Figure 24 (c) is when along the horizontal cross-sectional view when core dissects.The pick off part that this biosensor is different from Figure 23 is that cover plate 458 and dividing plate 456 are configured to measurement electrode 452 and electrode galvanic couple 453 can expose in the end of biosensor.Although not shown, being electrically connected between the circuit 11 of setting up main body 2 inside by the insertion of slide plate 6 and measurement electrode 452 and the electrode galvanic couple 453.Therefore, the insertion of slide plate 6 can be detected.Although Figure 24 has shown a configuration, wherein used measurement electrode 452 and electrode galvanic couple 453, the electrode that is independent of measurement electrode 452 and electrode galvanic couple 453 can be provided for detecting and insert.
(inserting the installation of keeper)
Then, similarly, insert keeper 207 (referring to Figure 13) by the chimeric body 2 that is inserted into.Insert keeper 207 and be provided with otch 214, this otch 214 is arranged so that user can not make a mistake direction of insertion.Although used otch at this, also can use other shape of projection for example or a plurality of otch or projection, it inserts the keeper asymmetry.
(installation of biosensor)
Then, user inserts in the keeper 207 by chimeric biosensor 8 is inserted into.Second opening 463 of biosensor 8 and the 3rd opening 464 are arranged so that they can be entrenched on first projection 215, second projection 216 and the 3rd projection 217 of inserting keeper 207.Because second opening 463 asymmetricly is arranged in the biosensor 8, user can insert biosensor and not mistaken direction of insertion.With reference to Figure 23, be provided with one second opening 463.Yet the present invention is not limited only to this, also can use the various configurations of satisfying above-mentioned purpose.The cross sectional shape of the 3rd opening 464 and second opening 463 can also be a different shape, for example except the part stripping and slicing of two circles, also has circle, rectangle, square, polygon and ellipse.In the situation of the shape of second opening 463 that changes biosensor 8 and the 3rd opening 464 and structure, need not, shape, structure and the quantity of inserting the projection that comprises first projection 215, second projection 216 and the 3rd projection 217 of keeper 207 also correspondingly change.
When biosensor by chimeric insertion in the described insertion keeper 207 time, electrode galvanic couple 453 in the 3rd opening 464 of biosensor 8 and measurement electrode 452 are electrically connected respectively with the second electrode (not shown) and first electrode 218 of the periphery of the cylinder 213 that inserts keeper 207.And, although it is not shown in Fig. 1 to 3, the terminal that is used for first electrode 218 and second electrode is arranged on the sidepiece of main body 2 sides, and pass through chimeric first electrode 218 that is inserted into main body 2 and second electrode that make to insert keeper 207 are electrically connected with circuit 11 in the main body 2.If biosensor 8 and main body 2 enter mutual status of electrically connecting, apparatus for measuring concentration of components 1 will be in laseropuncture and from the readiness of the measuring concentration of components of circuit 11, and the fact that this device has reached this state is presented on the display 3, makes the user can recognize this fact thus.
Subsequently, user is attached to skin on the film 454 of biosensor 8, makes predetermined laseropuncture point can arrive base openings 460.Simultaneously, insert the projection of keeper 207, first projection 215, second projection 216 and the 3rd projection 217 protrude about 1 to 3mm from biosensor 8, and these projections press skins.This will stimulate predetermined laseropuncture point around.Therefore, this can alleviate the stimulation that user is felt during laseropuncture.
(laseropuncture)
After user is attached to biosensor 8 to skin, finish the preparation of laseropuncture thus, user is pressed laser operations button 5, to carry out laseropuncture.Laser aid 9 utilizes laser operations button 5 from the signal of circuit 11 generations and the electric energy oscillating laser bundle of battery 10.The laser beam of vibration is focused on by collecting lens 12, and the skin of puncture user.
Wavelength for laser beam, wavelength with high skin absorbs coefficient is preferred, and 3 mu m wavebands (the middle infrared semiconductor laser of optically pumped laser or er-doped medium), or 9 to 10 mu m wavebands (the middle infrared semiconductor laser of discharge excitation laser or carbon dioxide gas body medium) are operable.In addition, the optically focused diameter of the laser beam on the skin is preferably below the φ 0.5mm, and it more preferably is below the φ 0.15mm.Although this optically focused shape is preferably circle, also can use its major axis to be set at the following ellipse of 0.5mm.Laser beam preferably vibrates in the mode of pulse, and the time width of the waveform of pulse is preferably more than the 1 μ s and below the 400 μ s.The irradiation of laser beam can be the irradiation of pulse bundle or the irradiation of multiple-pulse bundle.
For collecting lens 12, by at calcium fluoride (CaF
2), carry out non-reflection on the substrate of yttrium-aluminium-garnet (YAG), germanium (Ge), zinc selenide (ZnSe), anhydrous synthetic quartz and the manufacturing of fluoride moulding material and apply (dielectric multilayer-film) and obtain collecting lens, and its focal length is preferably 5 to 30mm.Use the calcium fluoride substrate in this embodiment.In addition, collecting lens 12 is roughly circle, and its overall diameter is 6 to 30mm.
(measurement of constituent concentration)
Behind laseropuncture, user from biosensor 8 move apart skin and push point of puncture around, with extrude 0.5 to several microliters of blood as the sample specimen.Therefore, select the sample reagent service duct 457 of a side of contact biosensor 8 by making blood sampling, airport 459 actions, blood is collected in the biosensor 8 by capillarity thus.For the blood of sampling, the material to be measured in the blood of reagent and sampling is reaction each other in reagent layer 455.For the electric charge that produces in the reaction, circuit 11 is via the electrode analysis quantity of electric charge of measurement electrode 452, electrode galvanic couple 453 and insertion keeper 207.Be presented on the display 3 by the concentration of analyzing the material to be measured in the blood that obtains, and discerned by user.Circuit 11 also comprises unshowned memory element, and analysis result was recorded together with measurement data and time or user code.Use display switch button 4, user can be discerned measurement result in the past afterwards once more.
In this embodiment, after biosensor at first was used as slide plate 6, the biosensor that is used as slide plate 6 in aforementioned measurement was used as biosensor 8 in ensuing composition measurement.In addition, because user is replaced biosensor and taken multiple measurements in one day when each composition measurement, user is prepared the biosensor 8 of tens of blade units.Therefore, it also helps only to use one of the dozens of biosensor and is used for slide plate.
The 11 embodiment
And, can also be provided for isolating opening and film for biosensor as the slide plate of Figure 26.Figure 26 (a) is a plane graph, and Figure 26 (b) is when the cross-sectional view when core dissects longitudinally, and 26 (c) are when along the horizontal cross-sectional view when core dissects.The biosensor part that this biosensor is different from Figure 23 and 24 is, wherein is provided with the opening 465 that passes substrate 451, dividing plate 456, cover plate 458 and film 466.Opening 465 can have the diameter that a laser beam does not have crested basically, and its minimum diameter is 1mm.And with reference to Figure 26, these openings are circular.Yet, can be for example rectangle, square, polygon and oval-shaped different shape.Although the shape of the opening 465 in substrate 451, dividing plate 456 and the cover plate 458 is identical, they can be the different shapes that laser beam does not have crested basically.
Selective membrane 466 from the base material group identical with film 454.Use cycloolefine polymer in this embodiment.Wish to increase the transmittance of film 466,, also can not form through hole therein even when making film be used laser beam repeatedly to shine for laser beam.And, be similar to aforementioned films 454, need not, it is useful giving film 466 deodorization functions and antibacterial characteristics.
Apparatus for measuring concentration of components according to this embodiment of the invention, main body is provided with laser aid, collecting lens, can analyzes the circuit of composition, can the display analysis result display and rechargeable battery, slide plate and insert keeper and be inserted in the main body on the laser beam optical axis, biosensor is entrenched in and inserts in the keeper, and slide plate also can be used as biosensor.Therefore, because user does not smell the abnormal flavour in the plume that produces in the laseropuncture process, and do not have to be polluted by plume yet, can realize simple and be easy to using as the optical element of beam condensing unit, that is, user can utilize a kind of consumable articles to manage sheet and reagent paper.
In addition, be provided with film in the slide plate that also has the biosensor function, this film is arranged on the optical axis of laser beam, is not pierced through by laser beam when as slide plate and is pierced through by laser beam when as biosensor.Thus, because user does not smell the abnormal flavour in the plume that produces in the laseropuncture process, and do not have to be polluted by plume yet, can realize simple and be easy to using as the optical element of beam condensing unit, that is, user can utilize a kind of consumable articles to manage sheet and reagent paper.
And, in the slide plate that also has the biosensor function, be provided with when the optical axis of laser beam is taken in as biosensor not film that is pierced through by laser beam and the film that when the optical axis of laser beam is taken in as slide plate, is not pierced through by laser beam.Thus, because user does not smell the abnormal flavour in the plume that produces in the laseropuncture process, and do not have to be polluted by plume yet, can realize simple and be easy to using as the optical element of beam condensing unit, that is, user can utilize a kind of consumable articles to manage sheet and reagent paper.
And, detect slide plate and be inserted into main functions by providing, can prevent that user from forgetting the insertion of slide plate, and correspondingly, can prevent reliably that collecting lens from being polluted by plume.
And the insertion operation aperture by slide plate to be to carry out the measuring ability that slide plate is inserted into main body, can prevent that user from forgetting the insertion of slide plate, and correspondingly, can prevent reliably that collecting lens from being polluted by plume.
And, by be arranged on slide plate in being connected of electrode can carry out the measuring ability that slide plate is inserted into main body, can prevent that user from forgetting the insertion of slide plate, and correspondingly, can prevent reliably that collecting lens from being polluted by plume.
And deodorization functions is arranged on the film of the slide plate that has the biosensor function on the optical axis of laser beam, is inserting the inner surface of keeper and the film of the biosensor on the optical axis at laser beam similarly on the optical axis of laser beam at least similarly.Thus, can eliminate abnormal flavour in the plume that produces in the laseropuncture process.
And, by in slide plate with biosensor function and insertion keeper, providing antibacterial functions, can prevent the infection when being used by user.
In addition,, in addition, for the sample specimen, can use these for example blood and interstitial fluids, and, can use blood glucose levels (concentration of glucose) for analyte via the sampling of skin for the sample specimen, or various serum or biochemical project.
In addition, as in the situation of analyte, as can be as the example of reagent, the enzyme combination of coenzyme and medium in some cases comprises: glucoseoxidase+ferricyanic acid potassium or ferricynium at blood glucose levels (concentration of glucose); And glucoseoxidase+pyrroloquinoline quinine or nicotinamide adenine+phenanthroline quinine, osmium comple or potassiumferricyanide.
The 12 embodiment
Hereinafter, will describe the example of laseropuncture device, wherein can be provided with the puncture of the 12nd embodiment of the present invention with installing.
Laseropuncture device according to the present invention comprises laser oscillator, be used for the display of display power supply, battery and mode of operation, setting operation pattern the setting button and wherein be provided with the main body and the interchangeable puncture of the operating operation switch that starts puncture procedure.For puncture; in ducted body with the axis that is parallel to laser beam axis; the collecting lens protecting film is arranged in the opening of the ducted body on the sidepiece of collecting lens; and the puncture film is arranged on the sidepiece of the position that the skin of point of puncture in the laseropuncture process is pressed, in the relative opening of ducted body.
By adopting such configuration, puncture film, ducted body and collecting lens protecting film form the space of sealing.By in the puncture film, forming the hole with laser beam, pass this hole laseropuncture skin, the plume of generation can be limited in the space of this sealing, and the deodorization functions that abnormal flavour can be set in puncture film, ducted body and the collecting lens protecting film is removed.In addition, owing to also be provided with antibacterial functions, needn't worry to infect.And because this adapter can be replaced, the replacement adapter is protected from infection in the time of can be by each uses.
Figure 27 (a) is the top view of laseropuncture device 1 according to this embodiment of the invention, and Figure 27 (b) is the schematic section that shows the internal structure of laseropuncture device.Laseropuncture device 1 comprises main body 2, comprises the laser oscillator 9 of operating power, collecting lens 12, puncture 507, panel 11, battery 10, console switch 5, set button 4 and display 3.Battery 10, panel 11 and laser oscillator 9, panel 11 and laser oscillator 9, setting button 4, display 3 are connected with signal with console switch 5 electricity.
Figure 28 is the sketch map of the puncture 507 in the laseropuncture device 1 of this embodiment of the present invention.This puncture 507 has the shape based on cylinder 513.Material for adapter, can use various plastic materials, they are polyethylene, polrvinyl chloride, polypropylene, polystyrene, polyvinyl acetate, ABS resin, AS resin, acrylic resin, polyacetals, polyimide resin, Merlon, modification polyphenylene oxide (PPE), polybutyleneterephthalate (PPB), polyarylate, polysulfones, poly-inferior benzene sulfide, polyether-ether-ketone, fluororesin etc.The material that has low electrokinetic potential on frosting is preferred.
The film 508 (first film) that punctures is arranged in the opening of people's skin (finger etc.) contact portion.Puncture film 508 absorbs laser beam and is punctured.For the base material of film 508, can use nylon, polyester, polyimides, fluorine system, vinyl chloride, polystyrene, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.
Opening on the sidepiece of main body is equipped with collecting lens protecting film 506 (second film).Collecting lens protecting film 506 does not absorb laser beam, but transmits this laser beam.Because this film is not by the absorption of laser beam puncture, unlike puncture film 508 (first film), it has the function that prevents to adhere to from the fragment of tissue of skin evaporation in the laseropuncture process collecting lens 12 and pollution collecting lens 12.For the base material of collecting lens protecting film 506, can use nylon, polyester, polyimides, fluorine system, vinyl chloride, polystyrene, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.
Then, operation will be described.At first, when user pressing operation switch 5, laseropuncture device 1 begins to start.Then, the operating condition of button 4 input laseropuncture devices 1 is set in the user utilization.After input, user is inserted into puncture 507 in the main body 2, and is his/her finger that the laseropuncture point is pressed on the puncture 507.
After finishing preparation, when " awaiting orders " is presented on the display 3, and user is once more during pressing operation switch 5, laser oscillator 9 oscillating laser pulsed beams, the laser beam of vibration is focused on by collecting lens 12, and focus on the skin of finger of user its beam diameter setting simultaneously less and be arranged in hollow puncture 507.Then, laser beam is by skin absorbs, and skin is punctured thus by gradually heat and evaporation.By puncture, the evaporation of the epidermis of skin and the upper space of its corium, for example the capillary tube in the skin mastoid process of corium is by wound, and blood oozes out.Then, skin surface is oozed out in the blood hole of passing puncture.
At this moment, do not have the hole to be formed in the collecting lens protecting film 506, the puncture film 508 of laser beam at the contact skin place is absorbed simultaneously, forms the hole thus.Pass this hole, carry out the aforementioned laser puncture.The plume that percutaneous evaporation produces in the laseropuncture process is disseminated in the ducted body 513 by the hole of puncture film 508.
The volatile material that is produced by the proteinic composition aminoacid that constitutes skin histology is decomposed and is flowing in the plume.Main target horny layer in the laseropuncture process is made up of the fibrous tissue of keratin protein, and fiber is by the cystine linkage combination, and this key is the key of the sulfur part of amino acid cystine, and it is more relatively that it comprises.Because these aminoacid decompose in evaporation process, produce volatile sulfur compounds or nitrogen component, especially, the people feels as the sulphur compound of abnormal flavour.
Therefore, be set to the inboard of puncture 507 for the deodorization functions of the smell component in the plume.Hydrogen sulfide or methyl mercaptan are mentioned as main sulphur compound.Hydrogen sulfide can pass through the chemosorbent deodorization, and can use manganese, copper and cobaltic composite oxygen compound.In addition, similarly, comprise any one oxide, hydroxide, composite oxygen compound or its mixture of manganese, copper, zinc and cobalt, can obtain adsorbent for the effective chemical Absorption of hydrogen sulfide by use.
Chemosorbent is absorbing hydrogen sulphide chemically, finally is the form with sulfate or simple sulfur material.In addition, chemosorbent also has instrumentality, and the methyl mercaptan of the sulfenyl abnormal flavour that its conversion is same is the dimethyl disulfide with higher thresholds.Because this effect, the people can feel and is equivalent to the deodorant effect.
And because dimethyl disulfide can be removed by the physical absorbent effect of using the physical absorbent that loads, it can remove sulphur compound substantially.Hydrophobic zeolite with major part silicon can be used as physical absorbent.In addition, even use zeolite, meerschaum, Silicon stone, aluminium oxide etc., also can obtain identical effect.
These chemosorbents and physical absorbent add the base material of collecting lens film 506, puncture film 508 or puncture 507 to.Perhaps, absorbent can be coated on the inside of collecting lens film 506, puncture film 508 or puncture 507.
In addition, because the finger of puncture film 508 contact user preferably gives the film antibacterial characteristics.Antibacterial characteristics can by apply or mixing on base material or within realize.These antibacterial comprise Ag, Cu, Zn, Ni, Co or their alloy, TiO
2, ZnO, WO
3Deng.In addition, antibacterial characteristics is by not only being immersed on the puncture film 508 but also on the outer surface of the ducted body 513 of puncture 507 and effectively.
Figure 29 (a) is the sketch map of another puncture 514 in the laseropuncture device 1 of embodiments of the invention.For puncture 514, projection 515 controls of the diameter of the opening that skin presses by forming around puncture film 508.Because the diameter of pushing to skin of realizing ideal depends on the shape setting of projection 515, can obtain reliable blood sampling.
Figure 29 (b) is the zoomed-in view of laseropuncture point during the laseropuncture.User is installed to puncture 514 the predetermined laseropuncture point of skin 516.At this moment, to 3mm, this projection 515 will be pushed skin 516 to projection 515 from puncture film 508 projectioies about 1.This will stimulate predetermined laseropuncture point around.Therefore, can alleviate the stimulation that user is felt in the laseropuncture process.
As mentioned above, according to the laseropuncture device 1 of this embodiment, puncture film 508, ducted body 513 and collecting lens protecting film 506 can form the space of sealing.Then, by form the hole with laser beam in puncture film 508, pass this hole laseropuncture skin, the plume of generation can be limited in the space of this sealing.
In addition, abnormal flavour can be set at the deodorization functions removal in puncture film 508, ducted body 513 and the collecting lens protecting film 506.In addition, owing to also be provided with antibacterial functions, needn't worry to infect.And because puncture 507 or 514 can be replaced, the replacement adapter is protected from infection in the time of can be by each uses.
The 13 embodiment
Hereinafter, with the laser irradiating method of describing as the 13rd embodiment of the present invention with laser beam puncture (laseropuncture) human body skin.After at first describing laser irradiating method, the laseropuncture device will be described.
Figure 30 is the sketch map according to the laser irradiation condition of the laseropuncture device of the 13rd embodiment of the present invention.In this view, the abscissa express time, vertical coordinate is represented laser beam intensity.Left side laser pulse bundle L1 is used to puncture the laser pulse bundle of epidermis, and right laser pulse bundle L2 is used to puncture the laser pulse bundle of corium.
The total time width T1 of laser pulse bundle L1 of epidermis of being used to puncture is 100 to 400 μ s.The total time width T3 of laser pulse bundle L2 of corium of being used to puncture is 50 to 300 μ s.The total time width T1 of laser pulse bundle L1 and L2 and poor (T1-T3) between the T3 are preferably 50 to 200 μ s.In addition, the interval between laser pulse bundle L1 and the laser pulse bundle L2 is short more good more.This is preferably below the 500ms at interval, and more preferably is below the 1ms.
For the energy of laser pulse bundle, when using two laser pulse bundle L1 and L2 unit of exposure area (1cm
2) time energy summation that obtains be preferably 100 to 300J.For example, if the diameter of irradiated area be 0.1mm (=0.01cm), area is 7.85 * 10
-5Cm
2(=0.005 * 0.005 * π).Energy intensity 100 by area being multiply by every unit of exposure is to 300J/cm
2Obtain 7.85 * 10
-5Cm
2* 100 to 300J/cm
2=7.85 to 23.6mJ become the energy summation of laser pulse bundle L1 and L2.
In addition, be used to puncture energy and being used to that the energy of laser pulse bundle of corium is preferably the laser pulse bundle of the epidermis that is used to puncture puncture corium the laser pulse bundle energy the energy summation 10% to 40%.That is, if the energy summation of laser pulse bundle L1 and L2 is 20mJ, the energy of the laser pulse bundle of the corium that then is used to puncture is preferably 10% to 40% (2 to 8mJ) of this energy summation.
And the optically focused diameter of laser pulse bundle is below the 0.15mm, to be preferably below the 0.1mm.In addition, the interval T2 between laser pulse bundle L1 and the L2 is short more good more.This is spaced apart below the 500ms, and is preferably below the 1ms.
Figure 31 is the zoomed-in view of the puncturing hole of the skin in the laser irradiation condition of laseropuncture device according to an embodiment of the invention.When skin was used to puncture the first laser pulse bundle L1 irradiation of epidermis, the cylindricality puncturing hole was formed in the epidermis, as Figure 31 (a).
Then, when the position identical with the first laser pulse bundle L1 was used to puncture the second laser pulse bundle L2 irradiation of corium, mortar shape puncturing hole was formed in the corium, as Figure 31 (b).Simultaneously, the capillary tube in the corium is by wound, and blood oozes out.The blood that oozes out oozes out via the laseropuncture hole of epidermis.The laseropuncture hole is 0.05 to 0.3mm to the degree of depth of corium, and preferably 0.05 to 0.25mm.
Figure 32 has shown other laser irradiation condition according to the 13rd embodiment of the present invention.The first laser pulse bundle L1 of the epidermis that is used to puncture shown in Figure 30 further is divided into a plurality of laser pulse bundle L3, L4 and L5.Be used to puncture laser beam L3, the L4 of epidermis and time width T4, T6 and the T8 of L5 is respectively 100 to 400 μ s.
In addition, laser pulse bundle L3, L4, L5 and L6 unit of exposure area (1cm
2) the energy summation that obtains is preferably 5 to 100J (energy intensity of every unit of exposure area is 5 to 100J/cm
2).The interval of laser pulse bundle L3, L4, L5 and L6 is short more good more.This is spaced apart below the 500ms, and is preferably below the 1ms.
Figure 33 is the sketch map that is contained in the laser oscillator 620 on the laseropuncture device according to an embodiment of the invention.Laser oscillator 620 comprises: laser bar 625, and the mirror film 623 and 624 that is used for the laser beam resonator is formed on its end; The flash lamp 621 that is used for excitation laser beam; Be used for effectively the light of flash lamp 621 being directed to the lampshade 622 of laser bar 625.In addition, also be useful on the power supply (not shown) of operation flash lamp 621.In addition, laser bar 625 and two mirror films 623 and 624 are formed the laser beam resonators.
The white light that sends from flash lamp 621 is directly reflected or in lampshade 722 inside, and the wavelength of the absorption of laser bar 625 is absorbed by laser bar 625.Then, the active medium in the laser bar 625 is energized, and the counter-rotating distribution forms.Spontaneous emission light is at laser bar 625 internal communications, and quilt is in the mirror film 623 and 624 reflections of two ends of laser bar 625, pattern with characteristic solution in optical resonator moves back and forth, and sensed emission amplification, and is drawn as laser beam from a low slightly mirror film 623 of reflection coefficient.
By trigger electrode ionizing in advance, by filling the high voltage electricity in capacitor, during xenon gaseous discharge in the glass tubing, flash lamp 621 sends white light.For a plurality of laser pulse bundles that vibrate, flash lamp 621 should be repeatedly luminous.This can realize, gate control high voltage electricity for example by once charge by gated semiconductor switch (IGBT), and when charging, carry out gate control, feasible repeatedly luminous method.By the inspection door control model, can control number, total time width, light energy and the pulse spacing of the pulse of each laser pulse bundle.
Figure 34 is the sketch map of laseropuncture device 1 according to an embodiment of the invention.The inside of main body is provided with the collecting lens 12 on the laser beam axis 632 of structure oscillation device 620, unshowned power supply and laser oscillator 620.Opening 633 is arranged in the focus of the collecting lens 12 on the laser beam axis 632 of main body.By finger being attached to opening and carrying out laseropuncture, can carry out blood sampling from finger.
As mentioned above, according to the laseropuncture method of this embodiment, the laser pulse bundle of the corium that is used to puncture shines the position with the laser pulse Shu Xiangtong of the epidermis that is used to puncture, and mortar shape puncturing hole is formed in the corium.Therefore, it is darker that corium is not punctured, and it can not stay scar, and the pain in the piercing process is less, and can carry out stable blood sampling.
In addition, the time width of the laser pulse bundle of the epidermis that is used to puncture is equal to or greater than the time width of the laser pulse bundle of the corium that is used to puncture, and the energy of the laser pulse bundle of the epidermis that is used to puncture is greater than the energy of the laser pulse bundle of the corium that is used to puncture.Therefore, the degree of depth of the cross sectional shape in the taper laseropuncture hole in the corium can be controlled to necessary minima, and can not stay cicatrix, and the pain in the piercing process is less, and can carry out stable blood sampling.
The 14 embodiment
At first, will the research contents that the present invention has invented be described.The present invention has found the following fact as the result of pain in the research blood sampling process.That is, the puncture of skin that is used for the people of blood sampling is directly carried out by pin and is carried out indirectly by laser beam.If the interaction of generation and human body can give the reaction of human body for effect.In addition, the testing agency of pain is that it detects pain by the nerves reaction for the stimulation of the free teleneuron of the corium on the sidepiece of epidermis.When the nerves reaction amount was higher than the pain detection threshold, pain was detected.
That is,, make the nerves reaction amount be lower than the pain detection threshold, perhaps consider to make fully high device of pain detection threshold in order to realize painless puncture.In order to make the nerves reaction amount less, wish to puncture, not stimulate other free teleneuron in the position that does not have free teleneuron.Yet as common blood sampling point, finger tip has the free teleneuron of higher density, and is not easy to avoid.
Simultaneously, also confirmed is that the pain detection threshold can be from external control to some degree.That is, find that the effect that reduces the pain detection threshold is along with the people changes for the consciousness that stimulates.This consciousness can be lowered by people's consciousness being focused on puncture opportunity in addition.
Especially, find, when the laseropuncture device uses, give periodically sound of user simultaneously, for example operating sound of pump or electromotor sound, the consciousness of user is focused on this periodicity sound, but not the puncture opportunity, and the pain that causes by stimulation during the laseropuncture feel be lowered.
In addition, can prepare the periodicity sound of any type, and randomly changing at every turn.In addition, the randomly changing at every turn of the time from the generation of periodicity sound to laseropuncture.This be because, if user is always heard identical periodicity sound, then can not focus on the consciousness of user on this periodicity sound, and if to arrive time of laseropuncture always identical, then user can by mistake be recognized the opportunity of laseropuncture.
Figure 35 is the sketch map of laseropuncture device 1 according to an embodiment of the invention.This laseropuncture device 1 comprises main body 2, comprises the laser oscillator 9 of operating power, collecting lens 12, puncture medicated cap 507, panel 11, battery 10, console switch 5, set button 4, display 3 and speaker 517.Battery 10, panel 11 and laser oscillator 9, panel 11 and laser oscillator 9, setting button 4, display 3, console switch 5 are connected with signal with speaker 517 electricity.The control signal that speaker 517 produces based on inside produces periodically sound, and is as mentioned below.
Figure 36 is the flow chart of the operation of laseropuncture device 1 according to an embodiment of the invention.This operation will be described according to this flow chart.At first, during user push switch 5 (step S11), laseropuncture device 1 begins to start.Then, user is by setting button 4 initial conditions, for example laseropuncture condition (step S12).
After setting, user is pressed in his/her finger as point of puncture on the puncture medicated cap 507.Simultaneously, puncture medicated cap 507 can be replaced (step S13).Then, as user (step S14) during push switch 5 once more, periodically sound begins to send (step S17) from speaker 517, and the operating power of laser oscillator 9 enters armed state (step S15).
Then, the laser pulse bundle that laser oscillator 9 vibration has specific time sequence, the laser pulse bundle of vibration are focused on and focus on by collecting lens 12 on the skin of finger of user, and its beam diameter is provided with lessly simultaneously, is arranged in hollow puncture medicated cap 507.Then, laser beam is by skin absorbs, and skin is heated and evaporates, and it is punctured (step S16) thus.
By puncture, the epidermis of skin and the evaporation of the outmost surface of its corium, for example the capillary tube in the skin mastoid process of corium is by wound, and blood oozes out.Then, blood oozes out the surface of skin by the hole of puncture.The periodically particular fixed week after date disappearance (step S18) of sound behind laseropuncture.
According to the laseropuncture device 1 of this embodiment, the consciousness of user is concentrated on the periodicity sound.Therefore, user can not waited for the opportunity of laseropuncture, and carries out laseropuncture under can be in the threshold value of the sensation of the pain lower state.Therefore, can ease the pain.
In addition, there are several periodicity sound, and the randomly changing at every turn of the time from the generation (step S17) of periodicity sound to laseropuncture (step S16).Therefore, user can not got used to this periodicity sound or time, and can concentrate on his/her consciousness on the laseropuncture thing in addition.
Figure 37 shows the quilt view of the example of the periodicity sound of laseropuncture device 1 use according to an embodiment of the invention.Here, periodically sound is meant dull multiple sound, the sound in the particular range in for example sound of pump or electromotor, or the repetition beat, for example metronomic sound.
Figure 37 (a) is the view of the intensity of the dull multiple sound of the sound of pump or electromotor for example.Dull multiple sound is low-frequency sound, and its mid frequency is 20 to 100Hz, and more preferably is 40 to 70Hz.Therefore, user can point to this sound with his consciousness, and does not feel to disperse.
Figure 37 (b) is the view of the intensity of the sound of the particular range with repetition beat of metronomic sound for example.For other sound with repetition beat, have mechanical switch ON/OFF sound, knock the sound of keyboard etc.If with the multiple beat of metronomic standard (per minute impacts how many times) expression is within 60 to 208 scope, and more preferably is 120 to 180.Therefore, user can point to bat to his consciousness and not sensory stimuli.
Figure 38 is the block diagram of internal structure that shows the laseropuncture device 1 of this embodiment.As shown in the drawing, laseropuncture device 1 is provided with laser oscillator 9 as sting device, display 3, console switch 5, sets button 4, CPU 721, be used for storing make CPU 721 carry out the program 719 of surrounding acoustic processing and periodically acoustic information 720 memorizer 718, be used to produce the periodicity sound processing unit 722 of sound periodically and as the speaker 517 of sound source.
In memorizer 718, a plurality of periodicity sound are stored as periodically acoustic information 720, and these sound for example be dull multiple sound, as the sound of the particular range of the sound of pump or electromotor and repetition beat, as metronomic sound.When the condition of laseropuncture etc. by when setting button 4 and set back console switchs 5 and be pressed, according to the program 719 that is stored in the memorizer 718, CPU 721 reads the periodicity acoustic information 720 of predetermined item from memorizer 718, it is corresponding with the random number that produces according to random function, and pass and send them to periodically sound processing unit 722, to produce predetermined periodicity sound from speaker 517.
After this, after through the random time that produces according to random function, CPU 721 makes skin by the laser pulse bundle irradiation from laser oscillator 9, punctures thus.Equally, in the laseropuncture device 1 of this embodiment, in piercing process, produce periodicity sound at random, and change the time from the generation of periodicity sound to the laser size randomly.Therefore, the consciousness of user can be disperseed from puncture.In addition, make user be difficult to wait for the opportunity of laseropuncture.Thus, the pain in the piercing process can not alleviate.
As mentioned above, according to the laseropuncture device 1 of this embodiment, produce periodic emulation sound, the consciousness of user is transferred to this sound, and makes user not recognize puncture opportunity.Therefore, can under the state of the threshold level of user feels pain, carry out laseropuncture.
In addition, in piercing process, produce random and periodical sound, and change the time from the generation of periodicity sound to laseropuncture randomly.Therefore, the consciousness of user can be shifted from puncture.In addition, user is difficult to wait for the opportunity of laseropuncture.Therefore, the pain in the piercing process can be alleviated.
The 15 embodiment
Before describing embodiments of the invention, at first, will the progress that the present invention has reached be described.Laseropuncture by laser pulse Shu Jinhang is the epidermis that is aimed at by puncturing, the capillary tube in the intradermal skin mastoid process of destruction, make blood ooze out from capillary tube, and skin is oozed out in the hole that makes blood pass in the epidermis.In addition, the laser pulse bundle is because skin is heated and evaporation by the systemic laser pulse beam energy of skin to the realization of the laseropuncture of skin.
The main component of skin is a water, and selection has the wavelength of the laser pulse bundle of high water absorption coefficient.For example, use the wavelength of 2.94 μ m of Er:YAG solid-state laser medium to be absorbed water most effectively, and can allow to use minimum laser pulse beam energy to puncture.
Therefore, the formative factor of the form between the individuality in the laser pulse beam energy that requires of laseropuncture comprises the thickness of epidermis of skin and the water content of epidermis.Usually, the thickness of corium becomes big with the age, and man's corium is thicker than the woman, and the relation of the thickness of the colour of skin and skin is that casting skin is the thickest, and yellow skin second is thick, and pale skin is the thinnest.In addition, the thickness of skin is also relevant with the frequency of utilization of people's finger tip, and uses the musician of its finger tip and athlete to have the skin thicker than common people.
And negative related although the water content of epidermis and the thickness of epidermis also have, it has the thickness individual difference more at random than epidermis.In addition, because there are fluctuation in the water content of the upper space of epidermis and the wetting balance of surrounding between sky and the sky and within one day.
In addition, because result of study of the present invention has proved that if utilize and traditional littler optically focused diameter (＜φ 0.15mm) of laseropuncture device, because individual variation, this water content has bigger effect than the thickness of epidermis for laseropuncture.Prove that at the optically focused diameter place of for example φ 0.5mm, the laser pulse beam energy that laseropuncture requires has the difference of about twice, and at the optically focused diameter place of φ 0.15mm, almost can't see difference.Find,, also have bigger difference although the thickness of epidermis is little.Yet, in the water content of epidermis, do not have difference.
Because above research, the laseropuncture device is configured according to an embodiment of the invention, makes water content sensor can be arranged in the puncture medicated cap of the skin that contacts user.Therefore, the water content of point of puncture can be in addition measured before puncture, and the attribute of user is imported in advance as age, sex, ethnic group and skin matter (thick, hard, thin and soft).
In addition, from the age, the data of sex, ethnic group and skin matter, with the laser pulse beam energy setup parameter comparison in the memorizer that is stored in the laseropuncture device, setting value is presented, and allows reliable blood sampling thus.
And, can also be at the laseropuncture device inner or its humidity sensor is set on surperficial, with the humidity of measurement environment air, to determine setting value.In addition, the pick off of measuring water content can be arranged on the surface of laseropuncture device and is punctured on the head.
Figure 39 is in the laseropuncture device 1 of embodiments of the invention, is provided with the sketch map of the puncture medicated cap 507 of moisture measurement pick off.Puncture medicated cap 507 has the shape based on cylinder 513, and the opening of the medicated cap on the sidepiece of main body is equipped with collecting lens protecting film 506.Collecting lens protecting film 506 does not absorb laser beam, but the propagated laser bundle.This film has during the laseropuncture from the fragment of tissue of skin evaporation and adheres on the collecting lens 12 and pollute this lens 12.
For the base material of this collecting lens protecting film 506, can use nylon, polyester, polyimides, fluorine system, vinyl chloride, polystyrene, polyethylene, polyolefin, polyvinyl alcohol, polypropylene etc.
Opening is arranged on the part that punctures that is used to of contact finger, and this opening is equipped with puncture film 508.Puncture film 508 propagated laser bundles, and absorb laser beam and be punctured.And the sensor electrode 821 that is used to measure water content is set up around around the puncture film.In puncture medicated cap 507, sensor electrode 821 is fixed to ducted body 513, and the surface-coated of sensor electrode has insulant glass.In addition, coupling part 814 is electrically connected sensor electrode 821 and apparatus main body 2.In addition, coupling part 814 can be arranged on the outer peripheral face or the inner peripheral surface of ducted body 513.
When this puncture medicated cap 507 was attached on the skin, electric capacity formed, and the horny layer of its mesocuticle is particularly as dielectric.Although the static capacity of this electric capacity is by the dielectric constant decision as the skin of dielectric, this dielectric constant depends critically upon moisture content of skin.This is because the ratio dielectric constant of water is about 80, compares greatly with for example proteinic dielectric constant about 1.5 of dielectric constant that forms cuticular other material.Therefore, this static capacity has reflected moisture content of skin significantly, and if the moisture content of skin change, the numerical value of static capacity also significantly changes.
Figure 40 (a) is the cross-sectional view that puncture medicated cap 507 is attached to the state of skin.At the end of puncture medicated cap 507, sensor electrode 821 is fixed to substrate, and the surface-coated of pick off has insulant glass.When this puncture medicated cap 507 was attached to skin, as shown in the drawing, sensor electrode 821 did not directly contact skin, but via insulant glass contact skin.
On the other hand, people's to be detected skin is made up of sebum layer 826, horny layer 827 and epidermis 828 successively from the surface.When sensor electrode 821 was attached to skin, the capacitor that its mesocuticle 827 is used as dielectric formed C as shown in FIG..Although the static capacity C of this capacitor is by the dielectric constant decision of dielectric skin, this dielectric constant depends critically upon moisture content of skin.
The static capacity checkout gear can produce the square wave that has by the frequency of static capacity and resistance value decision, and to transform this frequency of vibration be moisture content of skin.Figure 40 (b) is a schematic block diagram of explaining the static capacity checkout gear of the change be used to obtain static capacity C.
In the figure, especially, the part that is used to detect static capacity illustrates the object lesson of circuit configuration.Because the C of this attached middle school is the static capacity between the sensor electrode 821, and changes with moisture content of skin, it is described to variable capacitance.Circuit is the multi-frequency generator that the NOR door of known C-MOS is formed.
This circuit can produce the square wave that has by the frequency of C and R decision.This circuit is configured so that the change of static capacity C can be used as the change of frequency of oscillation and is obtained.This square wave is input in the microcomputer 819 as the moisture content of skin accountant, and microcomputer 819 these frequencies of conversion are moisture content of skin, and it is stored in the memorizer 820.And microcomputer 819 is regulated the laser pulse beam energy according to water content.
In addition, puncture medicated cap 507 is arranged at state, and this puncture medicated cap is released by spring and is used for accurately measuring water content, and is designed so that and can measures water content by pushing.This mechanism utilizes the interlocking of laseropuncture device 1 to connect, and be adapted to collecting lens 12 and finger skin between distance can be existing really by the high duplication Herba Stellariae Saxatilis.In addition, puncture medicated cap 507 is supported separably by the medicated cap keeper 831 of sting device 1 main body.
Figure 41 is near the cross-sectional view of puncture medicated cap 507.Puncture medicated cap 507 is connected to medicated cap keeper 831, and medicated cap keeper 831 keeps by the shell 833 of being formed shell as elastomeric spring 832.By such configuration, medicated cap keeper 831 is configured, with in the part of puncture medicated cap 507 when being compressed against on the skin, by the elasticity of spring 832 towards the slides within of shell 833.
That is, although puncture medicated cap 507 usually by spring 832 to extrapolation because flange 835 contact shells 833, the medicated cap 507 that punctures is in the state that does not jump.When puncture medicated cap 507 is pressed on the skin, puncture medicated cap 507 recessed shell inside, and constant by the spring pressure maintenance.If it is suitable that spring pressure is adjusted ground, sensor electrode 821 contacts skin by fixed pressure, and has therefore improved measuring stability.
Then, operation will be described.Figure 42 has shown the use flow process of laseropuncture device 1 according to an embodiment of the invention.At first, user pressing operation switch 5 (step S21), laseropuncture device 1 begin to start.Then, if user uses laseropuncture device 1 (not having step S22) first, user uses setting button 4 to import for example feature (step S23) of age, sex, ethnic group and skin matter (thick, hard, thin and soft).After input, user is pressed his/her finger on medicated cap (step S24) at full tilt fully.Then, the measurement (step S25) of beginning moisture content of skin.
After the measurement of moisture content of skin, be set (step S26) according to the measurement result of information of importing previously and moisture content of skin as the laser pulse beam energy of laseropuncture condition.Be shown among Figure 43 about the method for setting.At first, because the proportion function of laser pulse beam energy roughly is prepared according to initial conditions, the laser pulse beam energy correspondingly is set up.
Figure 43 (a) shows how to come setting laser pulsed beams energy according to initial conditions.As shown in the figure, high or low according to the age in advance, sex be man or woman (binary), ethnic group whether white people or yellow or Black people's (colour of skin) or skin matter be thin or thick soft or hard, prepare the laser pulse beam energy.For this relation, can be ratio, sum of powers exponential relationship.
Figure 43 (b) be in the embodiment of the invention laser pulse beam energy based on the measurement result of moisture content of skin and the explanation view of situation about being corrected.Proofreaied and correct along the vertical direction in this view based on the measurement result of moisture content of skin according to the numerical value that top initial conditions is set, and the laser pulse beam energy that will radiate is determined.At this moment, if water content is more, this numerical value is proofreaied and correct downwards, but and proofreaies and correct to making laser pulse beam energy step-down.
After correction is finished, when " awaiting orders " is presented on the display 3, and user is once more during push switch 5 (step S27), laser oscillator oscillating laser pulsed beams, the laser beam of vibration is focused on by collecting lens 12, and focus on the skin of finger of user, its beam diameter is provided with lessly simultaneously, is positioned at hollow puncture medicated cap 507.Then, laser beam is by skin absorbs, and skin is heated and evaporates, and is punctured (step S28) thus.
By puncture, the evaporation of the epidermis of skin and the upper space of its corium, for example the capillary tube in the skin mastoid process of corium is by wound, and blood oozes out.Then, the blood that oozes out passes puncturing hole and oozes out skin surface.In addition, if identical user reuses biosensor later on, owing to former initial conditions is stored, and the processing ("Yes" of step S22) after using for the second time is from the measurement of moisture content of skin.
Therefore, user can be realized blood sampling reliably and not by the individual variation in the laser pulse beam energy, everyday fluctuation and influence of fluctuations in a day simple and easyly, and does not have the laseropuncture of sampling blood in advance.
Figure 44 (a) illustrates example, and wherein, in laseropuncture device 1 according to an embodiment of the invention, the sensor electrode 821 of measuring water content is arranged on the surface of the laseropuncture device 1 except puncture medicated cap 507.In this case, before puncturing, finger tip is attached to the sensor electrode 821 that is used to measure water content, and water content is measured subsequently.According to the laseropuncture device of this embodiment, be arranged on the surface of laseropuncture device 1 owing to measure the sensor electrode 821 of water content, moisture content of skin can be measured simple and easyly.
And, can also be at the laseropuncture device inner or its humidity sensor is set on surperficial, with the humidity of measurement environment air, to determine setting value.Figure 44 (b) illustrates example, and wherein, in laseropuncture device 1 according to an embodiment of the invention, the pick off 822 of the humidity of measurement environment air is arranged on the surface of laseropuncture device 1.Because the humidity and the moisture content of skin of surrounding air are closely related, if the energy of laser pulse bundle is proofreaied and correct according to the humidity of surrounding air, the minimum laser pulsed beams energy that is suitable for blood sampling can be set exactly then.
Figure 45 is the zoomed-in view of the laseropuncture point during the laseropuncture.At first, when user push switch 5, laseropuncture device 1 begins to start.Then, user uses the operating condition of setting button 4 input laseropuncture devices 1.After input, user inserts main body 2 to puncture medicated cap 507, and its his/her finger as point of puncture is pressed on the puncture medicated cap 507.Then, moisture content of skin is measured, and is set as the laser pulse beam energy of the laseropuncture condition measurement result according to front input information and moisture content of skin.
After being ready to complete, when " awaiting orders " is presented at display 3 and user once more during push switch 5, laser oscillator 9 oscillating laser pulsed beams, the laser beam of vibration is focused on by collecting lens 12, and focus on the skin of finger of user, its beam diameter is provided with lessly simultaneously, to be positioned at hollow puncture medicated cap 507.Then, laser beam is by skin absorbs, and skin is heated and evaporates, and is punctured thus.By puncture, the evaporation of the epidermis of skin and the upper space of its corium, for example the capillary tube in the skin mastoid process of corium is by wound, and blood oozes out.Then, the blood that oozes out passes puncturing hole and oozes out skin surface.
At this moment, do not have the hole to be formed in the collecting lens protecting film 506, and laser beam is absorbed, and forms the hole thus by the puncture film 508 at contact skin place.Pass this hole, laseropuncture is performed the preceding.The hole that the plume that produces owing to the skin evaporation during laseropuncture passes puncture film 508 is diffused in the ducted body 513.
The volatile material that is produced by the proteinic composition aminoacid that constitutes skin histology is decomposed and is flowing in the plume.Main target horny layer in the laseropuncture process is made up of the fibrous tissue of keratin protein, and fiber is by the cystine linkage combination, and this key is the key of the sulfur part of amino acid cystine, and it is more relatively that it comprises.Because these aminoacid decompose in evaporation process, produce volatile sulfur compounds or nitrogen component, the people feels as the sulphur compound of abnormal flavour.
Therefore, be set to the inside of puncture 507 for the deodorization functions of the smell component in the plume.Hydrogen sulfide or methyl mercaptan are mentioned as main sulphur compound.Hydrogen sulfide can pass through the chemosorbent deodorization, and can use manganese, copper and cobaltic composite oxygen compound.In addition, similarly, comprise any one oxide, hydroxide, composite oxygen compound or its mixture of manganese, copper, zinc and cobalt, can obtain adsorbent for the effective chemical Absorption of hydrogen sulfide by use.
Chemosorbent is absorbing hydrogen sulphide chemically, finally is the form with sulfate or simple sulfur material.In addition, chemosorbent also has instrumentality, and the methyl mercaptan of the sulfenyl abnormal flavour that its conversion is same is the dimethyl disulfide with higher thresholds.Because this effect, the people can feel and is equivalent to the deodorant effect.
And because dimethyl disulfide can be removed by the physical absorbent effect of using the physical absorbent that loads, it can remove sulphur compound substantially.Hydrophobic zeolite with major part silicon can be used as physical absorbent.In addition, even use zeolite, meerschaum, Silicon stone, aluminium oxide etc., also can obtain identical effect.
These chemosorbents and physical absorbent can be by adding collecting lens film 506, puncture film 508 or puncture medicated cap 507 to.Or absorbent can be coated on the inside of collecting lens film 506, puncture film 508 or puncture medicated cap 507.
In addition, because the finger of puncture film 508 contact user preferably gives the film antibacterial characteristics.Antibacterial characteristics can by apply or mixing on base material or within realize.These antibacterial comprise Ag, Cu, Zn, Ni, Co or their alloy, TiO
2, ZnO, WO
3Deng.In addition, antibacterial characteristics is by not only being implanted on the puncture film 508 but also on the outer surface of ducted body 513 of puncture medicated cap 507 and effectively.
As mentioned above, according to laseropuncture device 1 and the laseropuncture method of this embodiment, the laser pulse beam energy is conditioned according to the moisture content of skin that measures by the pick off 821 of measuring moisture content of skin.Therefore, can set ideal laser pulse beam energy according to the fluctuation of moisture content of skin, and can carry out reliable blood sampling and the test of not puncturing, and almost not have pain.
In addition, be provided with the setting button, the attribute of its input user, as age, sex, ethnic group or skin matter, and the laser pulse beam energy is according to being conditioned from setting button inputted age, sex, ethnic group or skin matter.Therefore, can set ideal laser pulse beam energy by skin condition, and can almost not have the reliable blood sampling of pain.
The 16 embodiment
The 16th embodiment of the present invention designs a kind of new shape and inserts body, and wherein sheet, insertion body and reagent paper are one.When the laseropuncture device that does not have the measuring concentration of components function was considered, configured in one piece and film that film inserts body also allowed.
Figure 46 is the detailed perspective view of the insertion keeper 907 of the modification in the 16th embodiment of the present invention.In the figure, reference marker 913 expression ducted bodies, labelling 906 expression films, labelling 908 expression biosensors, labelling 914 expressions first electrode, labelling 915 expressions second electrode.
It is identical with for example insertion keeper 207 according to the 5th embodiment (Figure 13) to insert keeper 907, and the opening of the insertion keeper 907 on the main body is equipped with film 906.Film 906 is identical with the film 33 of slide plate 6.
Figure 47 is the biosensor sticking patch type insertion keeper according to this embodiment.In the figure, labelling 913 expression ducted bodies, labelling 906 expression collecting lens protecting film, labelling 908 expression biosensors, labelling 914 expressions first electrode, labelling 916 expression finger holder films, labelling 917 expression sample reagent service duct openings.Thereby the one group of electrode that is electrically connected with the analytic unit of main body is formed on the periphery of ducted body 913 and electrochemically uses biosensor, and is made of first electrode 914 and unshowned second electrode.
Collecting lens protecting film 906 is formed on the main body sidepiece that inserts keeper 907 and on the optical axis of laser beam, to comprise laser beam.Biosensor 908 is attached on the optical axis of laser beam on the sidepiece relative with main body that inserts keeper 907, to comprise laser beam.
In the part of optical axis that comprises biosensor 908 and laser beam, finger holder film 916 is formed on the position of wanting irradiated skin to press.In addition, be used for supplying the blood that oozes out from skin behind the laseropuncture and wait until that the sample reagent service duct of biosensor 908 is formed on the side of biosensor 908.Finger holder film 916 is the films that absorb laser beam and be punctured.On finger holder film 916, irradiated part can be fixed and can puncture with being stabilized by the handle finger pressure.
Figure 48 is the detailed view of biosensor 908 of Figure 47 and center sectional drawing (in addition, Figure 14's of the various piece of this configuration and the 5th embodiment is identical).For first electrode 914 of biosensor 908 and Figure 47 ducted body 913 peripheries and the second electrode (not shown) are electrically connected, each electrodes exposed on the sidepiece of biosensor 908 is on periphery on the sidepiece of biosensor 908, and these electrodes contact with each other during attaching.
Figure 49 is the sketch map that the hollow biosensor type inserts keeper 907, and wherein biosensor bends to ducted body and their end is connected, and collecting lens protecting film and finger holder film are arranged on two ends of ducted body.In the figure; reference marker 913,908 expression ducted body and biosensors; reference marker 906 expression collecting lens protecting film; reference marker 914 expressions first electrode; reference marker 916 expression finger holder films; reference marker 917 expression sample reagent service duct openings, reference marker 918 expressions second electrode, and reference marker 919 expression airports.
The acquisition of this keeper is identical with insulating barrier by the size of dividing plate that makes traditional biosensor (for example Figure 59) and cover plate, make first electrode 914 and second electrode 918 be exposed to dividing plate and cover plate, and otch is set to bend to ducted body at ducted body and to be made for the insertion keeper holding portion that can be fitted to main body when inserting keeper.The insertion keeper side of the insertion keeper holding portion of electrodes exposed on the sidepiece of unshowned main body, and when inserting keeper 907 and be engaged allows and being electrically connected of each electrode of biosensor.
Collecting lens protecting film 906 is attached on the main body sidepiece of making the intravital biosensor of hollow, and finger holder film 916 be attached to main body sidepiece facing surfaces on.In addition, sample reagent service duct opening 917 is arranged on the sidepiece of finger holder film 916, and airport 919 is arranged on the periphery of inserting keeper 907, makes the blood of sampling to flow by capillarity and passes the sample reagent runner.
The 17 embodiment
The 17th embodiment of the present invention relates to a kind of double-pulse laser pulsed beams, relates in particular to the puncture reagent paper film that utilizes the first weak emission and utilizes the last the second to launch the piercing method of skin puncture.
Figure 50 is the view that shows the oscillatory regime of the laser pulse bundle in the 17th embodiment of the present invention.As shown in figure 50, laseropuncture is by the first laser pulse bundle and the second laser pulse Shu Jinhang.Here, the light energy of the first laser pulse bundle and peak light intensity are set to be weaker than the light energy and the peak light intensity of the second laser pulse bundle.
Figure 51 (a) is the zoomed-in view of the postradiation laseropuncture point of the first laser pulse bundle in the 17th embodiment of the present invention, and Figure 51 (b) is the zoomed-in view of the postradiation laseropuncture point of the second laser pulse bundle.Laser beam is focused on the finger 18 of the user that carries out laseropuncture by collecting lens 12 and its place is the place 17 of laser beam.
The film 54 of the finger 18 contact biosensors 8 of user, and laser beam 17 propagates simultaneously from main body that its beam diameter little by little shrinks, as Figure 51 (a).Laser beam 17 is set to pass with propagation the opening 24 and the film 54 of the film 33, opening 32 of slide plate 6, the inside of inserting keeper 907, biosensor 8 and propagates, thereby focuses on the finger 18 of user.
The first laser pulse bundle tunicle 33 partly absorbs, but does not form through hole in the film, and laser beam 17 almost accumulates on the film 54 simultaneously, and its energy density is higher.Therefore, the laser beam tunicle absorbs with heating and evaporates this film, forms through hole (Figure 51 (a)) thus.Subsequently, the second laser pulse bundle with heating and evaporate skin, is finally destroyed the capillary tube of corium by the skin absorbs of the finger 18 of user thus, to allow blood sampling (Figure 51 (b)).
Therefore, in film 54, form through hole, and when having individual variation in the laseropuncture condition of skin, can allow stable blood sampling by regulating the second laser pulse bundle by the first laser pulse bundle.
The 18 embodiment
Figure 52 has shown the laseropuncture biosensor according to the 18th embodiment of the present invention.The biosensor of this embodiment is a biosensor, and wherein the sample reagent service duct is arranged on the sidepiece of film.Figure 52 (a) is a plane graph, and Figure 52 (b) is when the pick off of Figure 52 (a) cross-sectional view when core dissects longitudinally.In Figure 52, there is a feature, sample reagent service duct 307 is not connected with the outside of biosensor, but is connected with cover plate opening 311.In addition, because airport 309 is set in the face of the sample reagent service duct 307 across reagent layer 305, its position change.
Then, will utilize Figure 53 to describe operation.Figure 53 (a) is the view that shows pick off, finger and laser beam relation in use.Laser beam 13 tunicles 325 partly absorb, but do not form through hole in this film, and laser beam is collected on the film 304 simultaneously, to heat and to evaporate this film, form through hole thus.Subsequently, laser beam with heating and evaporate skin, is finally destroyed the capillary tube of corium by the skin absorbs of the finger 314 of user thus.
Figure 53 (b) is the view that shows the state behind the laseropuncture in detail.In Figure 53 (b), utilize laser beam in film 304, to form through hole.Similarly, the hole 316 of laseropuncture is formed in the skin of finger 314 of user.
The hole 316 that the blood that oozes out from the capillary tube of wound passes laseropuncture is exuded to the outside of finger 314.Utilize airport 309, the blood that oozes out passes sample reagent service duct 307 and is fed to reagent layer 305 by capillarity, so the constituent concentration in the blood can be measured.
According to this configuration, user can be supplied blood as measurement target to biosensor, does not take the sample reagent service duct to even he points his/her behind laseropuncture.
The 19 embodiment
Figure 54 has shown the zoomed-in view of the laseropuncture point behind the laseropuncture in the 19th embodiment of the present invention.In this embodiment, laseropuncture is to utilize the laser beam axis of the center deflection sample service duct of the film opening from the sidepiece of human body to carry out.In the figure, d-1 is the opening 310,311 and 312 and the centrage of 312 approximate centre of pick off of expression pick off, and d-2 is the laser beam axis at the center of expression laser beam 313.
In this embodiment, the central shaft d1 of the opening of pick off and laser beam axis d-2 are in different positions, although they are parallel.Especially, laser beam axis d-2 is set to the position of deflection sample reagent service duct 307.Laseropuncture carries out in the places that the finger 314 of laser beam axis d-2 and user intersects, and oozes out the outside of finger 314 from the blood that the capillary tube by the laseropuncture wound of the corium of skin oozes out.Because the more close sample reagent service duct 307 of blood ratio sensor open centre d-1 that oozes out can be more effectively by capillarity contact reagent layer 305.
The 20 embodiment
In the laseropuncture device of this embodiment, Figure 33's of laser oscillator 620 and the 13 embodiment is identical.This laser oscillator 620 comprises: laser bar 625, and the mirror film 623 and 624 that is used for the laser beam resonator is formed on its end; The flash lamp 621 that is used for excitation laser beam; Be used for the light of flash lamp 621 effectively is directed to the lampshade 622 of laser bar 625.In addition, also be useful on the power supply (not shown) of operation flash lamp 621.
The white light that sends from flash lamp 621 is directly reflected or in lampshade 722 inside, and the wavelength of the absorption of laser bar 625 is absorbed by laser bar 625.Then, the active medium in the laser bar 625 is energized, and the counter-rotating distribution forms.Spontaneous emission light is at laser bar 625 internal communications, and quilt is in the mirror film 623 and 624 reflections of two ends of laser bar 625, pattern with characteristic solution in optical resonator moves back and forth, and sensed emission amplification, and is drawn as laser beam from a low slightly mirror film of reflection coefficient.
By trigger electrode ionizing in advance, by filling the high voltage electricity in capacitor, during xenon gaseous discharge in the glass tubing, flash lamp 621 sends white light.Equally, if be provided with vacuum tube and have the high voltage electricity discharge, by the electrode sputtering phenomenon of discharge, the particle of sputter drifts about in xenon, and the emissive porwer of white light descends owing to particle optics absorbs.
In addition, because the shape of electrode changes by sputter, the space electronic supply status of (-) electrode that particularly comes to think highly of oneself changes, and the space homogeneity of discharge descends, and similarly, the emissive porwer of white light descends.And, as long as vacuum tube is provided, leaks and can not eliminate, even when the concentration of xenon changes, the emissive porwer of white light descends.Like this, the change of the emissive porwer of the flash lamp 621 of driving source reduces the output of laser oscillator 620, even and under identical imposing a condition, it is impossible that laseropuncture becomes.
In the laseropuncture device of this embodiment, be provided with the pick off of the white light of measuring flash lamp 621 emissions.By the peak value output of monitoring sensor, the peak value output and middle the comparing of obtaining that impose a condition.If difference exceeds constant value (for example 20%), inform that user state and the message that user is maintained sends.More preferably, can also not the still direct output of the flash lamp 621 of conduct input of emission by monitoring, carry out above-mentioned informing.
In addition, use the frequency of the sensor monitoring white light emission.Be imported into pick off if surpass the setting of specific settings triggering level, this is stored the device counting.This counting can show on the display of laseropuncture device and add up to and the count value in the fixed cycle (once restarting the back).
Correspondingly, if should count the stationary digital of the approaching number of times that adds up to, user is prompted the requirement maintenance, and if be somebody's turn to do the stationary digital of counting the number of times that surpasses total, may ban use of, and require the user maintenance.
In addition, for example, if diabetics uses this device to measure the blood sampling device of his blood glucose levels as being used to be in, with be used for controlling his/her blood glucose levels, for example insulin administration and oral administration, because the pin type sting device of existing sting device is simple and its element is less, it is very cheap.Owing to compare with pin type sting device, this laseropuncture device has inundatory many component parts, and it is difficult to provide with the price identical with pin type sting device.
Yet if use pin type sting device, each use will be bought new pin and replacement.Therefore, if, may make that prime cost is cheap even in the laseropuncture device, also allow to calculate rate according to access times.
For rate, the patient who uses insurance system to receive treatment carries this laseropuncture device, and for example, when he goes to hospital once every month regularly, the sanitarian can determine the number of times that uses, and increases and collect medical expense.In addition,, can periodically visit,, collect the charges to determine access times for maker or seller if use this puncture needle to exist in medical institutions.And the wired or wireless communication function of outer setting that can also give this laseropuncture device is determined access times to utilize this function from the outside, and is collected the charges to user.
Figure 55 is the laseropuncture schematic representation of apparatus according to this embodiment.This laseropuncture device comprises: main body 2, the laser head 952 of forming laser oscillator and Laser Power Devices 951, collecting lens 12, puncture medicated cap 507, panel 11, battery 10, console switch 5, setting button 4, display 3 and photoelectric sensor 950.Battery 10 is connected with signal with panel 11, Laser Power Devices 951 and photoelectric sensor 950, panel 11, Laser Power Devices 951, setting button 4, display 3, console switch 5 and photoelectric sensor 950 electricity.
Figure 56 has shown the use flow process of the laseropuncture device of present embodiment.Then, operation will be described.At first, when user pressing operation switch 5, laseropuncture device 1 begins to start (step S31).Then, the user utilization is set button 4 and is imported for example condition of laseropuncture condition (step S32).Simultaneously, the counting of the access times in the fixed cycle can be reset (step S41).Here, during fixed cycle in not advancing to the front flow process, the laseropuncture device will be in done state automatically.
After input, when user he/when his finger as point of puncture is pressed on the puncture medicated cap 507 (step S33), when " awaiting orders " is presented on the display 3, and when user once more during push switch 5 when (step S34), laser oscillator 34 oscillating laser pulsed beams, the laser beam of vibration is focused on by collecting lens 12, and focuses on the skin of finger of user, its beam diameter is provided with lessly simultaneously, to be arranged in hollow puncture medicated cap 507.Then, laser beam is by skin absorbs, and skin is punctured (step S35) thus by gradually heat and evaporation.By puncture, the evaporation of the epidermis of skin and the upper space of its corium, for example the capillary tube in the skin mastoid process of corium is by wound, and blood oozes out.Then, skin surface is oozed out in the blood hole of passing puncture.
Simultaneously, the photoelectric sensor in the laseropuncture device 950 is measured the photoemissive waveform (step S42) of emission white light with the flash lamp 621 of operation laser oscillator.In addition, be observed by the propagation waveform of collecting lens 12 laser light reflected pulsed beams.For photoelectric sensor 950, the pick off that has from the visible light of Si or InGaAs to the susceptiveness of the near infrared light that does not comprise the laser pulse bundle can be used for white light.
In addition, because the main component of target skin is a water, so selection has the wavelength of the laser pulse bundle of high water absorption coefficient.For example, use the wavelength of 2.94 μ m of Er:YAG solid-state laser medium to absorb most effectively in the water, and allow to utilize minimum laser pulse beam energy to puncture, therefore, pick off as the laser pulse bundle, the pick off that selection has susceptiveness and preferably at room temperature operates for infrared light, for example HgCdTe (MCT).
The light wave shape that photoelectric sensor 950 is measured is by following analysis.Figure 57 (a) is used for the view of method of interpretive analysis light wave shape.In this accompanying drawing, axis of abscissas express time, axis of ordinates are represented the light intensity that detects.In addition, Figure 57 (b) illustrates photoelectric sensor 950 and detects from the aspect from the light of laser head 952 emissions.
At first, if input surpasses default triggering level, then carry out waveform recording.Under conventional environment, in the photoelectric sensor 950 in being arranged on main body, the triggering level is set to triggering can be owing to outside white light or infrared light and effective level.Trigger if input surpasses this, the numeral of the counting of the memorizer in the panel 11 increases by 1 (step S43).
Then, similarly, wavy peak value is recorded in the memorizer in the panel 11 (step S45).Then, compare with the estimation peak value that is stored in advance in the memorizer based on the laseropuncture condition that is provided with by condition enactment.Then, the peak value of the peak value of measurement and estimation compares (step S46).Then, if the peak value of measuring is lower than the threshold level that the peak value one of estimation allows, the demonstration of the requirement maintenance of display 3 is performed.
In addition, if from the numeral of the comprehensive number of times brought into use numeral near the upper limit number of times the memorizer that is stored in advance similarly in the panel 11, the message that maintenance closes on is presented on the display 3, if and reached the numeral of the comprehensive number of times of the upper limit, the demonstration of the requirement maintenance of display 3 would be performed.If the display requirement maintenance can also be carried out the demonstration of forbidding then using and locking this device.
In order to show the numeral of the comprehensive number of times in total and fixed cycle, can do to set button 4 in the condition entry on-screen options, make them be shown thus.In addition, can setting code, make the reset numeral of the comprehensive number of times in the fixed cycle of person in charge only thus.And, although not shown, can by communication function externally is set, the numeral of the comprehensive number of times in the fixed cycle is read in for example wire communication (telephone wire or LAN circuit) or wireless telecommunications (mobile communication, infrared communication or WLAN) from the outside.
Although the present invention is described in detail with the reference specific embodiment, be clear that for those skilled in the art, do not leave the spirit and scope of the present invention can carry out various substitutions and modifications.The application requires the interests of Japanese patent application 2006-110673 that submits in the Japanese patent application No.2006-078430 that submitted on March 22nd, 2006, the Japanese patent application No.2006-082303 of submission on March 24th, 2006, on April 13rd, 2006 and the Japanese patent application 2006-111805 that submitted on April 14th, 2006, and it is quoted and is incorporated into this.
Industrial applicibility
As mentioned above, apparatus for measuring concentration of components according to the present invention has in the laser beam of the preventing piercing process The plume that comprises peculiar smell that produces leaks into the outside, processes the effect as the plume of smell component, and quilt As the apparatus for measuring concentration of components of biology sensor with the skin that can utilize laser beam puncture human body, and fast The quantity of the various special components very in a small amount in the biological specimen of speed and easily definite sampling.
Claims (70)
1. apparatus for measuring concentration of components comprises:
Main body, its have at least laser aid, the described laser beam of emission laser beam beam condensing unit, body fluid composition analytical equipment and show display device by described analytical equipment result calculated;
Sheet;
Insertion body with the opening that does not cover described laser beam; With
Reagent paper,
Wherein said main body is provided with opening along the optical axis direction of described laser beam,
Wherein said opening is equipped with described insertion body, and an end of described insertion body is equipped with described reagent paper, and
Wherein said provides the film between described beam condensing unit and described insertion body, and described film is not punctured by described laser beam.
2. apparatus for measuring concentration of components as claimed in claim 1, wherein said reagent paper is provided with the composition measurement electrode, described insertion body is provided with electrode, and the composition measurement of described reagent paper with the electrode of electrode and described insertion body by being electrically connected with described reagent paper is chimeric.
3. apparatus for measuring concentration of components as claimed in claim 1, wherein said analytical equipment are analyzed the composition of described body fluid by the enzyme reaction of sample reagent.
4. apparatus for measuring concentration of components as claimed in claim 3, wherein said analytical equipment are the Optical devices of analyzing the composition of described body fluid by the chromogenic reaction of sample reagent.
5. apparatus for measuring concentration of components as claimed in claim 1, wherein said reagent paper provides the film that is punctured by laser beam on the optical axis that is positioned at described laser beam.
6. apparatus for measuring concentration of components as claimed in claim 5, wherein said reagent paper also provides the film that is not punctured by laser beam on the optical axis that is positioned at described laser beam.
7. apparatus for measuring concentration of components as claimed in claim 1, wherein said reagent paper also has the function as described.
8. apparatus for measuring concentration of components as claimed in claim 7, wherein said reagent paper also provide and are used as described film on the optical axis that is positioned at described laser beam, and when as described, described film is not punctured by laser beam.
9. apparatus for measuring concentration of components as claimed in claim 1, the chimeric sidepiece between the main body of wherein described insertion body and the opening is provided with unsymmetric structure.
10. apparatus for measuring concentration of components as claimed in claim 1, chimeric between wherein said reagent paper and the described insertion body undertaken by unsymmetric structure.
11. apparatus for measuring concentration of components as claimed in claim 10, chimeric between wherein said reagent paper and the described insertion body are by chimeric the carrying out between the projection of the opening of described reagent paper and described insertion body.
12. apparatus for measuring concentration of components as claimed in claim 1, at least a portion of the film of wherein said film, described insertion body and described reagent paper or all be provided with deodorization functions.
13. apparatus for measuring concentration of components as claimed in claim 1, at least a portion of the film of wherein said reagent paper, described insertion body and described reagent paper or all be provided with antibacterial functions.
14. one kind is used for the puncture biosensor of composition of the sample specimen of collecting of analysis and utilization laser beam,
Wherein said pick off forms the sample service duct, and the sample specimen of being supplied is drawn onto between described first and second substrates by described sample service duct; By being fitted between described first and second substrates, filter and be arranged in the described sample service duct with the reagent of composition reaction in the described sample specimen; And lead to the air outside hole from described sample service duct and be arranged in described second substrate, and
Wherein said reagent has enzyme and chromogen, it specifically reacts with described composition, partly or entirely shared opening is arranged on the outside of the sample service duct in described first and second substrates, and any one opening at least of described first and second substrates is provided with film.
15. biosensor as claimed in claim 14, wherein said film has deodorization functions.
16. as claim 14 or 15 described biosensors, wherein said first and second substrates also are provided with the part or all of shared opening outside the described opening.
17. one kind is used for the puncture biosensor of composition of the sample specimen of collecting of analysis and utilization laser beam,
Wherein said pick off forms the sample service duct, and the sample specimen of being supplied is drawn onto between described first and second substrates by described sample service duct; At least by being fitted between described first and second substrates, be arranged in the described sample service duct with the reagent of composition reaction in the described sample specimen; And lead to the air outside hole from described sample service duct and be arranged in described second substrate,
Wherein said pick off has electrode system, and described electrode system comprises measurement electrode and electrode galvanic couple at least, and reacts and detected by described electrode system, and
Partly or entirely shared opening is arranged on the outside of the described sample service duct in described first and second substrates, and any one opening at least of described first and second substrates is provided with film.
18. laseropuncture biosensor as claimed in claim 17, wherein said film has deodorization functions.
19. as claim 17 or 18 described laseropuncture biosensors, wherein said first and second substrates also are provided with the part or all of shared opening outside the described opening.
20. as claim 17 or 18 described laseropuncture biosensors, wherein said first and second substrates also are provided with at least two the part or all of openings of sharing outside the described opening, make described electrode system to expose.
21. an apparatus for measuring concentration of components comprises:
Main body, its have at least laser aid, the described laser beam of emission laser beam beam condensing unit, body fluid composition analytical equipment and show display device by described analytical equipment result calculated;
Sheet;
Insertion body with opening that laser beam passes; With
Reagent paper,
Wherein said main body is provided with opening along the optical axis direction of described laser beam,
Wherein said opening is equipped with described insertion body, and an end of described insertion body is equipped with described reagent paper, and
Wherein said between described beam condensing unit and described insertion body, and described function with described reagent paper.
22. apparatus for measuring concentration of components as claimed in claim 21, wherein said provides the film on the optical axis that is positioned at described laser beam, and described film is not punctured by described laser beam as described the time, and is punctured by described laser beam when as described reagent paper.
23. apparatus for measuring concentration of components as claimed in claim 21, wherein said provide on the optical axis that is positioned at described laser beam be used as described reagent paper the time film that punctured by described laser beam, and provide on the optical axis that is positioned at described laser beam be used as described the time film that punctured by described laser beam.
24. apparatus for measuring concentration of components as claimed in claim 21 wherein also provides described of detection to be inserted into described main functions.
25. apparatus for measuring concentration of components as claimed in claim 24, wherein detecting described, to be inserted into described main functions be that the Mechanical Contact of the insertion by described is operated and carried out.
26. apparatus for measuring concentration of components as claimed in claim 24, wherein detecting described, to be inserted into described main functions be by being arranged on being electrically connected to fetch and carrying out of electrode in described.
27. as each described apparatus for measuring concentration of components of claim 21 to 26, at least one or two in wherein said film and the described insertion body are provided with deodorization functions.
28. as each described apparatus for measuring concentration of components of claim 21 to 27, at least one or two among wherein said insertion body and described are provided with antibacterial functions.
29. one kind is installed in the puncture that punctures thus on the laseropuncture device that utilizes laser beam irradiation skin, described adapter comprises:
The ducted body that has opening two ends; With
Setting is used for sealing first film and second film of the described opening of described two ends,
Wherein said first film absorbs laser beam and is punctured, the described second film transmission laser bundle and do not absorb laser beam.
30. having, puncture as claimed in claim 29, wherein said ducted body be formed on described first film projection on every side.
31. as claim 30 or 31 described punctures, at least one in wherein said first film, described ducted body and described second film is provided with deodorization functions.
32. as claim 30 or 31 described punctures, wherein said first film is provided with antibacterial functions.
33. a laseropuncture device that comprises separable as each described puncture of claim 29 to 32, the central shaft of described ducted body and laser beam axis are set to overlap each other.
34. one kind is utilized the irradiation of laser pulse bundle to comprise that the skin of epidermis and corium carries out the laser irradiating method of laseropuncture thus, described method comprises step:
Radiation be used to the to puncture laser pulse bundle of described epidermis; With
Radiation be used for the puncturing laser pulse bundle of corium of point of puncture of described epidermis.
35. laser irradiating method as claimed in claim 34, the time width of laser pulse bundle of described epidermis of wherein being used to puncture equals or is longer than to be used to the laser pulse bundle of corium that punctures, and the energy of the laser pulse bundle of the described epidermis that is used to puncture is greater than the laser pulse bundle of the corium that is used to puncture.
36. laser irradiating method as claimed in claim 34, the time width of the laser pulse bundle of the epidermis that wherein is used to puncture is 100 to 400 μ s, and the time width of the laser pulse bundle of the corium that is used to puncture is 50 to 300 μ s.
Difference 37. laser irradiating method as claimed in claim 34, the time width of the laser pulse bundle of the epidermis that wherein is used to puncture and being used to puncture between the time width of the laser pulse bundle of corium is 50 to 200 μ s.
Irradiation 38. laser irradiating method as claimed in claim 34, the laser pulse bundle of the epidermis that wherein is used to puncture and being used to puncture between the laser pulse bundle of corium is spaced apart below the 500ms.
Energy summation 39. laser irradiating method as claimed in claim 34, the laser pulse bundle of the epidermis that wherein is used to puncture and being used to puncture during the laser pulse bundle irradiation of corium be every square centimeter 100 to 300J.
40. laser irradiating method as claimed in claim 34, the energy of the laser pulse bundle of the corium that wherein is used to puncture be used to puncture epidermis and being used to puncture corium the laser pulse bundle the energy summation 10 to 40%.
41. laser irradiating method as claimed in claim 34, the optically focused diameter of wherein said laser pulse bundle is below the 0.15mm.
42. laser irradiating method as claimed in claim 34, the laser pulse bundle of the epidermis that wherein is used to puncture comprises a plurality of laser pulse bundles.
43. laser irradiating method as claimed in claim 42, the time width of each that a plurality of laser pulses of the epidermis that wherein is used to puncture are intrafascicular is 100 to 400 μ s.
44. laser irradiating method as claimed in claim 42, the irradiation of a plurality of laser pulse bundles of the epidermis that wherein is used to puncture is spaced apart below the 500ms.
Energy summation 45. laser irradiating method as claimed in claim 42, a plurality of laser pulse bundles of the epidermis that wherein is used to puncture and being used to puncture during the laser pulse bundle irradiation of corium be every square centimeter 5 to 100J.
46. a laseropuncture device that utilizes laser beam irradiation skin to puncture thus comprises:
Produce the periodically periodicity flexible piezoelectric sound-generating devices of sound,
Wherein puncture is carried out during producing described periodicity sound.
47. laseropuncture device as claimed in claim 46, wherein said periodicity sound are that its mid frequency is with 20 to 100Hz dull multiple sound.
48. laseropuncture device as claimed in claim 47, the multiple sound of wherein said dullness comprise the operation sound and the electromotor sound of pump.
49. laseropuncture device as claimed in claim 46, wherein said periodicity sound are to have the sound that per minute impacts 60 to 208 repetition beat.
50. laseropuncture device as claimed in claim 49, wherein said sound with repetition beat comprise metronomic sound, mechanical switch ON/OFF sound and knock the sound of keyboard.
51. laseropuncture device as claimed in claim 46, wherein said periodicity sound generating apparatus produces tonequality or different polytype periodicity sound of cycle, and changes the type of the periodicity sound that produces when at every turn puncturing randomly.
52. laseropuncture device as claimed in claim 46, wherein the time from the generation of periodicity sound to puncture changes when each puncture randomly.
53. a laseropuncture method of utilizing laser beam irradiation skin to puncture thus, described method comprises step:
From different polytype periodicity sound of tonequality or cycle, select and produce predetermined periodicity sound at random; And
Puncturing after from the generation of described predetermined periodicity sound through the time at random.
54. a laseropuncture device that utilizes laser beam irradiation skin to puncture thus, described device comprises step:
Adjusting device, its basis are regulated the energy of described laser pulse bundle by the moisture content of skin of the moisture measurement sensor measurement of measurement moisture content of skin.
55. laseropuncture device as claimed in claim 54 comprises described moisture measurement pick off.
56. laseropuncture device as claimed in claim 55, wherein said moisture measurement pick off comprises sensor electrode, and described sensor electrode is arranged on the surface of described laseropuncture device main body to measure moisture content of skin.
57. laseropuncture device as claimed in claim 54 comprises holding device, described holding device keeps having the puncture medicated cap of described moisture measurement pick off separably,
Wherein said puncture medicated cap is included in first film and second film that two ends have the ducted body of opening and are set to seal the opening of described two ends,
The wherein said first film transmission laser bundle also absorbs laser beam and is punctured, and the described second film transmission laser bundle and do not absorb laser beam, and
Wherein said moisture measurement pick off comprises the sensor electrode on the same level that is arranged on described first film, to measure moisture content of skin.
58. laseropuncture device as claimed in claim 54 comprises the setting button of the attribute of importing user, wherein said adjusting device is according to the energy of regulating described laser pulse bundle from the button inputted attribute of described setting.
59. laseropuncture device as claimed in claim 54 comprises the humidity sensor of the humidity of measurement environment air, wherein said adjusting device is regulated the energy of described laser pulse bundle according to the humidity of the surrounding air of described humidity sensor measurement.
60. a laseropuncture method of utilizing laser beam irradiation skin to puncture thus, described method comprises step:
Measure moisture content of skin; And
Regulate the energy of described laser pulse bundle according to the moisture content of skin of measuring.
61. one kind is installed in the insertion keeper that utilizes on the laseropuncture device that laser beam irradiation skin punctures thus, described insertion keeper comprises:
The ducted body that has opening two ends;
Be set to seal an end of described opening, and transmission laser bundle and do not absorb the film of laser beam;
Be set to seal the biosensor of the other end of described opening; And
Be arranged on the outer peripheral face of described ducted body or inner peripheral surface to be electrically connected the electrode of described laseropuncture device and described biosensor.
62. as the described insertion keeper of claim 67, wherein finger holder film is formed in the part surface of described biosensor, and sample reagent service duct opening is formed in the side of described biosensor.
63. insertion keeper as claimed in claim 61, wherein said biosensor have measurement electrode and the electrode galvanic couple that is formed in the reagent layer.
64. one kind is installed in the insertion keeper that utilizes on the laseropuncture device that laser beam irradiation skin punctures thus, described insertion keeper comprises:
By crooked biosensor be connected the ducted body that the end of biosensor forms;
Be set to seal an end of described ducted body, and transmission laser bundle and do not absorb the film of laser beam;
Be set to seal the other end of described ducted body, and the film that absorbs laser beam and be punctured; And
Be arranged on the outer peripheral face of described ducted body or inner peripheral surface to be electrically connected the electrode of described laseropuncture device and described biosensor.
65. one kind is utilized the laser pulse bundle to shine the laser irradiating method that skin carries out laseropuncture thus via film, described method comprises step:
Radiation be used to the to puncture first laser pulse bundle of described film; And
Radiation is used for the second laser pulse bundle in the point of puncture skin puncture of described film.
66. as the described laser irradiating method of claim 65, the intensity of the wherein said first laser pulse bundle is weaker than the intensity of the described second laser pulse bundle.
67. one kind is installed in the biosensor that utilizes on the laseropuncture device that laser beam irradiation skin punctures thus, described biosensor comprises:
First substrate, have attaching thereon reagent layer and have first opening that laser beam passes;
Second substrate is set to predetermined space to have second opening of corresponding described first opening in the face of described first substrate, and has and be positioned at than the airport of described reagent layer further from the position of described second opening; And
The sample reagent service duct is formed at from described first opening to described reagent layer described first substrate and described second substrate.
68. as the described biosensor of claim 67, the optical axis of wherein said laser beam is set to be parallel to the centrage of described first opening, and is positioned at the position than the more close described sample reagent service duct of centrage of described first opening.
69. the laseropuncture device that the laser beam irradiation skin that utilizes flash lamp to encourage punctures thus, comprising: optical pickocff, described optical pickocff detect the light emission of described flash lamp to monitor the degradation of described flash lamp.
70. as the described laseropuncture device of claim 69, comprise informing device, described informing device is informed the message of the described matching requirements maintenance of user based on the access times of the device that is detected by photoelectric sensor.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP078430/2006 | 2006-03-22 | ||
| JP2006078430 | 2006-03-22 | ||
| JP082303/2006 | 2006-03-24 | ||
| JP110673/2006 | 2006-04-13 | ||
| JP111805/2006 | 2006-04-14 |
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| Publication Number | Publication Date |
|---|---|
| CN101351153A true CN101351153A (en) | 2009-01-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007800010660A Pending CN101351153A (en) | 2006-03-22 | 2007-03-22 | Biosensor and apparatus for measuring concentration of components |
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| Country | Link |
|---|---|
| CN (1) | CN101351153A (en) |
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