CN101263783A - Method for using herbicide barban to fast breed aloe all-male plant strain - Google Patents
Method for using herbicide barban to fast breed aloe all-male plant strain Download PDFInfo
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- CN101263783A CN101263783A CNA2008100609033A CN200810060903A CN101263783A CN 101263783 A CN101263783 A CN 101263783A CN A2008100609033 A CNA2008100609033 A CN A2008100609033A CN 200810060903 A CN200810060903 A CN 200810060903A CN 101263783 A CN101263783 A CN 101263783A
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Abstract
The invention discloses a method utilizing herbicide Barban to rapidly cultivate asparagus all male plants strain, which comprises the following steps: 1) amphoteric strain is chosen from the asparagus varieties for production purpose; the amphoteric strain produces asparagus seeds by homologous strain inbred; 2) the asparagus seeds are soaked in Barban, and bud forcing treatment is carried out to the asparagus seeds; 3) the processed asparagus seeds are cultivated in a nutrition pot; flowering takes place after 30 to 35 days; each individual plant respectively hybrid with a female strain, and the seeds of each individual plant are reserved; 4) the seed reserved from each individual plant are processed again by Barban; after flowing, the ratio of male and female flowering is calculated, and super male plants are identified; 5) super male plant clones are reserved and propagated by in vitro culture method; 6) the super male plant is used as the male parent to hybrid excellent female line, and asparagus all male plants strain can be acquired. The method has the advantages that, the time of asparagus from seedling to flowering is greatly reduced; the identification process of super male plants is accelerated; the invention has an important role in the breeding of asparagus all male plants strain, and the application prospect is wide.
Description
Technical field
The invention belongs to crop varieties breeding technique field, specifically, the present invention relates to the technology of the full staminiferous plant breed breeding of a kind of asparagus, particularly a kind of method of utilizing weed killer herbicide barban to fast breed aloe all-male strain.
Background technology
Asparagus formal name used at school Asparagus officinalis L is the perennial perennial root herbaceous plant of Liliaceae Asparagus, is that vegetables are edible to take out living tender stem, asparagus edibility height, and the sweet fragrant deliciousness of food has flavour; The contained nutritive element of asparagus is very comprehensive and ratio is proper, and medical value is long and use extensive.Contain micro substances such as surprising abundant antioxidant, immunocyte activator and Normocellular growth regulator in the asparagus.Asparagus remarkable in economical benefits asparagus purposes is wide, remove the processing can, outside the nutriment, country and area all are extremely welcome vegetables to asparagus in America and Europe, Japan, Taiwan etc., be described as " one of the world's ten big famous dishes ", in recent years also be subjected to consumer's approval and favor gradually in China, market prospects are wide.
China introduced u.s. variety UC500 from 1974, and in Zhejiang, province such as Shandong, Liaoning, Fujian carry out commercial production plantation achieving success.The eighties in 20th century and the nineties, main Mary Washington and the UC series of products that adopts California, USA also introduced the U.S., Germany, Holland, Spain and Japan cultivars in succession in the production.UC157 for example, Idalea (love Delhi), Franklim (Franklin) etc., selecting for use of F1 hybrid began to draw attention in recent years, and for example Apollo (Apollo), the Glan base of a fruit (Grande), pool are to last (Jersey supreme), damp western giant (Jesey knight) etc.But China asparagus produces with planting almost entirely from abroad at present, and it is very unbecoming that this and asparagus are developed into a big industry in China, therefore wants our asparagus kind industry of develop actively.Along with the renewal of asparagus plant, the continuous import seed of still needing has from now on so not only increased production cost greatly, and seed purity is poor, have a strong impact on yield per unit area and quality, produce the China that develops on a large scale asparagus, this has become its very severe problem of advancing of obstruction.So China asparagus process of breeding and crossbreeding is voluntarily quickened in an urgent demand.
Asparagus is dioecious plant, the sex chromosome of female plant is XX, the sex chromosome of staminiferous plant is XY, and the staminiferous plant quality is good, output is high, under field conditions (factors), the ratio of male and female plant is 1: 1, if cultivate staminiferous plant flower pesticide with anther cultural method, can obtain the non-existent supermale strain of natural world (YY) after haplobiont doubles, supermale strain and normal plant mating, the offspring who obtains is staminiferous plant entirely, on producing very high economic worth is arranged.The output of complete male crossbreed comparable female originally/volume increase of male bulk variety is more than 30%.Complete male crossbreed grows consistent and unnecessary fruit is seldom arranged.Tanaka can not produce a large amount of volunteers in production, has exempted the trouble of artificial removing volunteer.Complete male crossbreed outward appearance homogeneous, local flavor is good, and disease resistance improves, and reduces weeds, and can go into operation in advance.Complete male kind is not owing to produce F
2For seed, thereby do not palm off seed.
In the full staminiferous plant breed breeding process, the cultivation of supermale strain and screening are very important.And the cultivation of supermale strain at present mainly obtains by two kinds of approach, the 2nd, and by anther culture or pollen cultured method, the 2nd, by teleianthous selfing.Although anther cultural method successfully has been applied to the seed selection of many kinds, also exist technical difficulty, for example anther culture regeneration frequency very low (on average having only 6.8), and different genotype differences very big (Falarigna et al 1985).By second method is that the hermaphrodite flower cross method is also very difficult, in a single day though obtain the hermaphrodite flower plant, 1/4 offspring is the supermale strain.Because asparagus is the dioecism plant, 1: 1 sex ratio (male: Mm; Female: mm).But general supermale strain and male strain are difficult to be distinguished on form, and a supermale strain need be distinguished from offspring's sex segregation ratio.Because blooming, male strain of confirmation and female strain need to broadcast in the greenhouse back 210-235 days and 269-295 days separately respectively.So utilize this system to identify that from hermaphrodite flower the supermale strain needs 24 months at present, this has seriously hindered the cultivation work of the complete male system of asparagus.And because it is the dioecism crop, selfing is unlikely, so form the very difficulty of kind of isozygotying of homogeneity by the mode of crossbreeding, because the stable yield evaluation needs the time in several years, so utilize conventional method to cultivate the time that asparagus new varieties often need more than ten years even decades.
Abe and kameya (1986) takes the lead in having found to utilize Atrazine and diuron to handle asparagus seeds can inducing seedling can bloom in one month after field planting.Also have report to show Triazines, Ureas, anilides, benzamides and carbarnates have similar effect (Abe et al., 1987; Hara et al., 1992; Ozaki etal., 1999).United States Patent (USP) (Patent 6174712) also provides a kind of fatty acid that promotes that asparagus blooms.Utilize the chemical reagent processing to induce the seedling of 1-2 month seedling age to bloom and to form each fast from generation to generation, can form pure lines at short notice, thereby shorten breeding process, in asparagus genetic research and breeding of new variety, be of great use.
It will be the main means of cultivating new varieties from now on that asparagus conventional breeding and modern biotechnology organically combine.The asparagus traditional breeding method is simple, and is workable, but the cycle is very long. and utilize tissue culture technique, can accelerate the seed selection progress of asparagus breeding greatly, simplify the procedure of breeding, shortening the breeding cycle.
List of references:
Abe?T.&T.Kameya.1986.Promotion?of?flower?formation?by?atrazine?anddiuron?in?seedlings?of?Asparagus.Planta?169：289-291.
Abe?T.，R.Shimizu，H.Iwamura&T.Kameya.1?987.Flower?induction?byatrazine?analogs?in?seedlings?of?Asparagus?officinalis.Physiol?Plant?70：228-230.
Hara?T.，N.Wada&H.Iwamura，1992.Flower?induction?in?asparagus?seedlingsby?anilide?and?benzamide?derivatives.J?Agric?Food?Chem?40：1692-1694.
Ozaki?Y.，T.Kurahashi，T.Tashiro&H.Okubo.1999.Carbamate?inducedflowering?in?asparagus(Asparagus?officinalis?L.)seedlings：optimization?of?treatmentand?cultivar?variation?in?flowering?response?and?pollen?germination.Euphytica?110：77-83.
Peng?MS，Ziauddin?A，Wolyn?D.Development?of?asparagus?microspores?in?vivoand?in?vitro?is?influenced?by?gametogenic?stage?and?cold?treatment?In?Vitro?Cellularand?Development?Biology-Plant.1997，33：263-268
Summary of the invention
The objective of the invention is to be difficult for evaluation, the main dependence on import of hybrid seed, to cost an arm and a leg and purity is difficult to guarantee this present situation, propose a kind of method of utilizing weed killer herbicide barban to fast breed aloe all-male strain at the breeding hybridized excessive cycle of present asparagus, supermale strain.The Ba Er plate claims oatax and barban again, and chemistry 4-chloro-2-butynyl-N-(3 chlorphenyl) carbamate by name belongs to a kind of chloropropham.
Utilize the method for weed killer herbicide barban to fast breed aloe all-male strain to comprise the steps:
1) select the both sexes strain from the asparagus kind of production usefulness, the selfing of both sexes strain homophyletic obtains asparagus seeds;
2) utilize the Ba Er plate to soak also vernalization and handle asparagus seeds;
3) treated asparagus seeds is grown seedlings in nutritive cube, grows to bloom after 30～35 days, and each individual plant with female strain hybridization, is reserved seed for planting by individual plant respectively;
4) the individual plant seed of reserving seed for planting utilizes the Ba Er plate to handle again, and the ratio of the back statistics male and female flowering of blooming is identified the supermale strain;
5) utilize in vitro culture method to preserve and breeding supermale strain clone;
6) utilize above-mentioned supermale strain can obtain the complete male strain of asparagus with good female series hybridization as male parent.
Described step 2) the vernalization methods for the treatment of seed comprises the steps: to utilize weed killer herbicide Ba Er plate to soak also
A) the Ba Er plate is dissolved in the dimethyl sulfoxide (DMSO), is dissolved in lentamente in the deionized water then, obtaining concentration is 50mgl
-1Ba Er plate solution;
B) put three metafiltration paper in the culture dish, add 20ml Ba Er plate solution, with two-layer gauze parcel asparagus seeds, cultivated 12 days at 25 ℃ of following irradiations, intensity of illumination is 54.2 μ mol
-1m
-2s
-1, the photoperiod is 12 hours, the back seed that germinates cleaned sowing 10～15 minutes in clear water.
Described step 4) identifies that the method for supermale strain comprises the steps:
C) utilize the Ba Er plate to soak also vernalization and handle asparagus seeds;
D) asparagus seeds after the germination is sowed in the nutritive cube, blooms the ratio of statistics male and female flowering after 30～35 days;
E) from male parent, identify the supermale strain according to the filial generation sexual type after blooming.
4, a kind of method of utilizing the full staminiferous plant strain of weed killer herbicide quickly breeding asparagus according to claim 1 is characterized in that the method for described step 5) cultured in vitro supermale strain comprises the steps:
F) the tender stem of supermale strain washed 30～45 minutes with running water, cleaned 10～20 minutes with washing agent, with 70% alcohol-pickled 5 minutes, handled 25 minutes with containing 0.1% mercuric chloride that 1-2 drips tween again, use rinsed with sterile water at last 3～4 times, after blotting material surface moisture with aseptic filter paper, cut 0.5～0.6 centimetre stem with bud, inoculate after peelling off the scale on axillalry bud surface;
G) stem with bud is inoculated into and is added with the 0.2mg/L 6-benzyladenine, 0.1mg/L methyl, evoking adventive bud on the MS medium of 25g/L sucrose and 6g/L agar;
H) indefinite bud was cultivated after 1 month, was transferred to contain 1.0mg/L paclobutrazol PP
333Root induction on the 1/2MS medium of+0.5mg/L indole-3-butyric acid+sucrose 25g/L+ agar 6g/L;
I) rooting tube plantlet carries out hardening transplanting to place the last week under the natural lighting, day by day unclamp blake bottle and seal film until removing at last, peat soil, vermiculite and perlite are mixed with 3: 1: 1 ratios, the sterilization back keeps the ambient humidity of ventilation and 80～90% as transplanting medium under the HTHP behind the test-tube seedling transplanting.
The beneficial effect that the present invention compared with prior art has:
1) utilize the method for the full staminiferous plant strain of carbamate compound quickly breeding asparagus greatly to shorten asparagus (reduced to about 35 days from broadcasting the back) from the seedling to the flowering time in more than 200 day, accelerated the qualification process of supermale strain, made the evaluation of supermale strain reduce to below 6 months from 24 months;
2) utilize carbamate compound to handle the ratio that asparagus seeds can improve male flower, in asparagus supermale strain screening, play an important role;
3) cultured in vitro supermale strain development supermale strain system can keep the good strains of seeds of supermale strain system on the one hand, can significantly reduce the supermale stable variety time on the other hand, accelerates the breeding process of full staminiferous plant kind;
4) the present invention has good economic and social benefit having obtained important breakthrough aspect the full staminiferous plant strain breeding of asparagus, has a extensive future.
Description of drawings
Accompanying drawing asparagus supermale strain Rapid identification process.
Embodiment
Utilize the method for the full staminiferous plant strain of weed killer herbicide quickly breeding asparagus to comprise the steps:
1) select the both sexes strain from the asparagus kind of production usefulness, the selfing of both sexes strain homophyletic obtains asparagus seeds;
Asparagus is dioecism normally, but the both sexes strain of 1-5% appears in regular meeting in some kind, finds also in some kind that the rate of setting seeds exactly likes the both sexes strain of staminiferous plant, the male both sexes strain of only a few.General staminiferous plant gynoecium style and column cap are degenerated fully, and ovary is degenerated only to stay and moved back mark.And male both sexes strain gynoecium style and stigma development are good, and ovary is not exclusively degenerated or grown normal.These hermaphrodite flowers can be set seeds, and the rate of setting seeds is about 2-10%.
Both sexes strain individual plant selfing is reserved seed for planting.In the both sexes strain that big Tanaka finds, get male flower pollen and carry out artificial pollination, it is pollinated on the teleianthous column cap of corresponding individual plant seed maturity results after about 50 days.Statistics ripening rate and seed number.
2) utilize weed killer herbicide Ba Er plate to soak also vernalization and handle asparagus seeds;
The Ba Er plate is dissolved in the dimethyl sulfoxide (DMSO), is dissolved in lentamente in the deionized water then, obtaining concentration is 50mgl
-1Ba Er plate solution;
Asparagus seeds is used 50mgl earlier
-1Ba Er plate solution soaking 8-10 hour; Put three metafiltration paper in culture dish, add 20ml Ba Er plate solution, with two-layer gauze parcel asparagus seeds, cultivated 12 days at 25 ℃ of following irradiations, intensity of illumination is 54.2 μ mol
-1m
-2s
-1, the photoperiod is 12 hours, the back seed that germinates cleaned sowing 10～15 minutes in clear water.
3) treated asparagus seeds is grown seedlings in nutritive cube, grows to bloom after 30～35 days, and each individual plant with female strain hybridization, is reserved seed for planting by individual plant respectively;
Select staminiferous plant and female plant because the selfing of both sexes strain homophyletic produces 3/4 staminiferous plant (Mm), and wherein 1/3 is homogeneity type staminiferous plant (MM), 2/3 is heterogeneous type staminiferous plant (Mm), 1/4 homogeneity type female plant (mm).Adopt the test cross method just homogeneity type staminiferous plant (MM) can be screened.Bloom in 30～35 days right sides behind the planting seed that the Ba Er plate is handled, and distinguishes staminiferous plant and female plant according to the flower type.
Test cross is reserved seed for planting above-mentioned staminiferous plant is numbered by individual plant, and male flower is carried out artificial hybridization with the female flower on the homogeneity female plant (mm).
4) the individual plant seed of reserving seed for planting utilizes the Ba Er plate to handle again, and the ratio of the back statistics male and female flowering of blooming is identified the supermale strain;
Utilize the Ba Er plate to soak and vernalization processing asparagus seeds; Asparagus seeds after the germination is sowed in the nutritive cube,
Divide the sub-district sowing by the staminiferous plant individual plant.Identify male flower and female flower, statistics numbers, calculating ratio in the time of after planting 30～35 days.If be male flower in the offspring, its male parent individual plant is the supermale strain; If female flower and male flower ratio were near 1: 1 in the offspring, then male parent is heterogeneous staminiferous plant.
5) utilize in vitro culture method to preserve and breeding supermale strain clone;
The tender stem of supermale strain washed 30～45 minutes with running water, cleaned 10～20 minutes with washing agent, with 70% alcohol-pickled 5 minutes, handled 25 minutes with containing 0.1% mercuric chloride that 1-2 drips tween again, use rinsed with sterile water at last 3～4 times, after blotting material surface moisture with aseptic filter paper, cut 0.5～0.6 centimetre stem with bud, inoculate after peelling off the scale on axillalry bud surface;
Stem with bud is inoculated into and is added with the 0.2mg/L 6-benzyladenine, 0.1mg/L methyl, evoking adventive bud on the MS medium of 25g/L sucrose and 6g/L agar;
Indefinite bud was cultivated after 1 month, was transferred to contain 1.0mg/L paclobutrazol PP
333Root induction on the 1/2MS medium of+0.5mg/L indole-3-butyric acid+sucrose 25g/L+ agar 6g/L;
Rooting tube plantlet carries out hardening transplanting to place the last week under the natural lighting, unclamp blake bottle day by day and seal film until removing at last.Peat soil, vermiculite and perlite are mixed with 3: 1: 1 ratios, and HTHP is used as transplanting medium in the sterilization back down.The ambient humidity that keeps ventilation and 80～90% behind the test-tube seedling transplanting.Water after surviving, sealing fertilizer 1 time, it is moistening that the seedbed keeps, and temperature keeps 25-30 ℃. and growing seedlings gets final product field planting to the land for growing field crops about 1 month.
6), preparation combination, select good full staminiferous plant strain
UC157, UC800, Atlas are introduced in the selection of homogeneity type female series, Apollo (U.S.), Lucullus (Germany), Franklim (France) etc. are to selecting the female series individual plant in the stronger kind of this province adaptability, through screening for many years, formed more stable female series.Selecting wherein, 4 good female series comprise as female parent: 991407,990712,002204,010207.
The perianth of preparing hybrid combination fresh flower and dry and cold storage is as hybridization pollen source.The fresh flower flower pesticide of gathering at once before blooming, the dried flower powder is stored in 3 ℃, can keep 12 days.Dry and cold storage can be used for adjusting the florescence.
Each makes up field comparative test each combination in conjunction with combining ability test and the acquisition of disease resistance evaluation and screening, carries out the continuous observation of 3 term output and quality characteristic.The commodity of field trial statistics output and tender stem comprises and takes out the par of giving birth to tender stem, weight, transverse diameter, color and luster, the degree of packing etc., thereby determines good hybrid combination.
Embodiment 1
1. found 9 strain both sexes strains the complete male production Tanaka of West Germany, wherein 6 strains have obtained 58 mature seeds after the homophyletic selfing artificial pollination.
2. asparagus seeds is used earlier 30ml 50mgl in flask
-1Ba Er plate solution soaking 8-10 hour; Put three metafiltration paper in culture dish, add 20ml Ba Er plate solution, with two-layer gauze parcel asparagus seeds, cultivated 12 days at 25 ℃ of following irradiations, intensity of illumination is 54.2 μ mol
-1m
-2s
-1, the photoperiod is 12 hours, the back seed that germinates cleaned sowing 10～15 minutes in clear water.
3. after planting grow seedlings, majority is bloomed after about 35 days, and flowering rate is 58.7%, distinguishes staminiferous plant and female plant according to the flower type.Staminiferous plant and female plant ratio are 21: 6 as a result, are higher than 3: 1 ratio of expection.Explanation is under carbamate cpd Ba Er plate is handled, and the staminiferous plant ratio significantly improves.
4. above-mentioned staminiferous plant is pressed the individual plant numbering, male flower is carried out artificial hybridization with the female flower on kind ' UC157 ' the homogeneity type female plant (mm) of life in 2 years, select another kind female plant to help improving its ripening rate.Each staminiferous plant is with 12 female plant test crosses.
5. divide the seed of individual plant results to utilize the Ba Er plate to handle by preceding method.Asparagus seeds is used earlier 30ml 50mgl in flask
-1Ba Er plate solution soaking 8-10 hour; Put three metafiltration paper in culture dish, add 20ml Ba Er plate solution, with two-layer gauze parcel asparagus seeds, cultivated 12 days at 25 ℃ of following irradiations, intensity of illumination is 54.2 μ mol
-1m
-2s
-1, the photoperiod is 12 hours, the back seed that germinates cleaned in clear water 10～15 minutes, the subregion sowing.Identify male flower and feminisation, statistics numbers, calculating ratio in the time of after planting 35 days.Found that 5 strains are that the test cross offspring is staminiferous plant or the overwhelming majority is male flower, infer that thus this 5 strain parent is the supermale strain.
6. the foundation of supermale strain vegetative propagation system
(1) the tender stem of supermale strain is fetched with running water flushing 30～45 minutes in the field, cleaned 10～20 minutes with washing agent, 70% alcohol-pickled 5 minutes, handled 25 minutes with containing 0.1% mercuric chloride that 1-2 drips tween again, last rinsed with sterile water 3～4 times, after aseptic filter paper blots material surface moisture, cut 0.5～0.6 centimetre stem with bud, inoculate after peelling off the scale on axillalry bud surface;
(2) be inoculated into and be added with the 0.2mg/L 6-benzyladenine, the 0.1mg/L methyl cultivates on the MS medium of 25g/L sucrose and 6g/L agar that indefinite bud occurs after 14 days.
(3) treat that indefinite bud grows to 4～5cm and is inoculated into when high and contains 1.0mg/L paclobutrazol PP
333Root induction on the 1/2MS medium of+0.5mg/L indole-3-butyric acid IBA+ sucrose 25g/L+ agar 6g/L.
Rooting tube plantlet carries out hardening transplanting to place the last week after (4) 40 days under the natural lighting, unclamps blake bottle day by day and seals film until removing at last.Peat soil, vermiculite and perlite are mixed with 3: 1: 1 ratios, and HTHP is used as transplanting medium in the sterilization back down.Transplant the back heat and moisture preserving, promote slow seedling.
(5) seedling management.Behind the slow seedling, can impose 10-15kg/667m
2Composite fertilizer, water immediately after the fertilising, as finding insect pest such as aphid, the control of agrochemicals such as available Rogor.At seedling raising process, need spray bactericide protections such as tpn, mancozeb.In matrix, cultivate after 30-35 days and get final product field planting.
(6) form the supermale strain system of 2 separate sources at last, be respectively ME14, MF12-3.
7. the utilization of supermale strain system
(1) the asparagus kind Lucullus (Germany) that introduces from Germany is through the screening of selfing for many years, and it is consistent to have formed a growth, stable female series, promptly 012204.
(2) the preparing hybrid combination male flower pollen of getting ME14 or MF12-3 is invested on 012204 the female flower style, treats to gather respectively behind the seed maturity statistics ripening rate.
(3) respectively make up the field comparative test with above combined grow in booth, carry out the sub-district comparative test, began to add up output in 1 year, its yield and quality estimated in continuous 3 years, select fine combination.
Claims (4)
1, a kind of method of utilizing weed killer herbicide barban to fast breed aloe all-male strain is characterized in that comprising the steps:
1) select the both sexes strain from the asparagus kind of production usefulness, the selfing of both sexes strain homophyletic obtains asparagus seeds;
2) utilize weed killer herbicide Ba Er plate to soak also vernalization and handle asparagus seeds;
3) treated asparagus seeds is grown seedlings in nutritive cube, grows to bloom after 30～35 days, and each individual plant with female strain hybridization, is reserved seed for planting by individual plant respectively;
4) the individual plant seed of reserving seed for planting utilizes weed killer herbicide Ba Er plate to handle again, and the ratio of the back statistics male and female flowering of blooming is identified the supermale strain;
5) utilize in vitro culture method to preserve and breeding supermale strain clone;
6) utilize above-mentioned supermale strain can obtain the complete male strain of asparagus with good female series hybridization as male parent.
2, a kind of method of utilizing weed killer herbicide barban to fast breed aloe all-male strain according to claim 1 is characterized in that described step 2) the vernalization methods for the treatment of seed comprises the steps: to utilize the Ba Er plate to soak also
A) the Ba Er plate is dissolved in the dimethyl sulfoxide (DMSO), is dissolved in lentamente in the deionized water then, obtaining concentration is 50mgl
-1Ba Er plate solution;
B) put three metafiltration paper in the culture dish, add 20ml Ba Er plate solution, with two-layer gauze parcel asparagus seeds, cultivated 12 days at 25 ℃ of following irradiations, intensity of illumination is 54.2 μ mol
-1m
-2s
-1, the photoperiod is 12 hours, the back seed that germinates cleaned sowing 10～15 minutes in clear water.
3, a kind of method of utilizing weed killer herbicide barban to fast breed aloe all-male strain according to claim 1 is characterized in that the method for described step 4) evaluation supermale strain comprises the steps:
C) utilize the Ba Er plate to soak also vernalization and handle asparagus seeds;
D) asparagus seeds after the germination is sowed in the nutritive cube, blooms the ratio of statistics male and female flowering after 30～35 days;
E) from male parent, identify the supermale strain according to the filial generation sexual type after blooming.
4, a kind of method of utilizing weed killer herbicide barban to fast breed aloe all-male strain according to claim 1 is characterized in that the method for described step 5) cultured in vitro supermale strain comprises the steps:
F) the tender stem of supermale strain washed 30～45 minutes with running water, cleaned 10～20 minutes with washing agent, with 70% alcohol-pickled 5 minutes, handled 25 minutes with containing 0.1% mercuric chloride that 1-2 drips tween again, use rinsed with sterile water at last 3～4 times, after blotting material surface moisture with aseptic filter paper, cut 0.5～0.6 centimetre stem with bud, inoculate after peelling off the scale on axillalry bud surface;
G) stem with bud is inoculated into and is added with the 0.2mg/L 6-benzyladenine, 0.1mg/L methyl, evoking adventive bud on the MS medium of 25g/L sucrose and 6g/L agar;
H) indefinite bud was cultivated after 1 month, was transferred to contain 1.0mg/L paclobutrazol PP
333Root induction on the 1/2MS medium of+0.5mg/L indole-3-butyric acid+sucrose 25g/L+ agar 6g/L;
I) rooting tube plantlet carries out hardening transplanting to place the last week under the natural lighting, day by day unclamp blake bottle and seal film until removing at last, peat soil, vermiculite and perlite are mixed with 3: 1: 1 ratios, the sterilization back keeps the ambient humidity of ventilation and 80～90% as transplanting medium under the HTHP behind the test-tube seedling transplanting.
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| CN107211892A (en) * | 2017-06-22 | 2017-09-29 | 中国热带农业科学院热带生物技术研究所 | A kind of MD2 pineapples root media and its tissue culture sprout quick breeding method |
| CN107439220A (en) * | 2017-08-17 | 2017-12-08 | 四川省农业科学院经济作物育种栽培研究所 | A kind of high yield cultivating method of the non-all-male kind of asparagus |
| CN107708408A (en) * | 2015-01-09 | 2018-02-16 | 利姆集团有限公司 | Sex determining gene and its purposes in breeding |
| CN110313396A (en) * | 2019-02-15 | 2019-10-11 | 山东省潍坊市农业科学院 | A method of supermale strain is obtained using asparagus male both sexes strain |
| CN116602206A (en) * | 2023-06-27 | 2023-08-18 | 山东省农业科学院 | Cultivation method of low-temperature-resistant asparagus variety |
-
2008
- 2008-03-25 CN CN2008100609033A patent/CN101263783B/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107708408A (en) * | 2015-01-09 | 2018-02-16 | 利姆集团有限公司 | Sex determining gene and its purposes in breeding |
| US11439072B2 (en) | 2015-01-09 | 2022-09-13 | Limgroup B.V. | Sex determination genes and their use in breeding |
| CN105724244A (en) * | 2016-04-20 | 2016-07-06 | 云南农业大学 | Method for conducting cross breeding through homozygous parent prepared through selfing of bisexual plant and supermale |
| CN107211892A (en) * | 2017-06-22 | 2017-09-29 | 中国热带农业科学院热带生物技术研究所 | A kind of MD2 pineapples root media and its tissue culture sprout quick breeding method |
| CN107439220A (en) * | 2017-08-17 | 2017-12-08 | 四川省农业科学院经济作物育种栽培研究所 | A kind of high yield cultivating method of the non-all-male kind of asparagus |
| CN107439220B (en) * | 2017-08-17 | 2020-02-18 | 四川省农业科学院经济作物育种栽培研究所 | High-yield cultivation method for asparagus |
| CN110313396A (en) * | 2019-02-15 | 2019-10-11 | 山东省潍坊市农业科学院 | A method of supermale strain is obtained using asparagus male both sexes strain |
| CN116602206A (en) * | 2023-06-27 | 2023-08-18 | 山东省农业科学院 | Cultivation method of low-temperature-resistant asparagus variety |
| CN116602206B (en) * | 2023-06-27 | 2024-05-03 | 山东省农业科学院 | Cultivation method of low-temperature-resistant asparagus variety |
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| CN101263783B (en) | 2012-02-29 |
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