CN101181540A - Application of pharmaceutical composition in the preparation of resistance prostatitis - Google Patents
Application of pharmaceutical composition in the preparation of resistance prostatitis Download PDFInfo
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Abstract
The invention relates to an application of a medicine compound in the preparation of a medicine for treating prostatitis, belonging to the Chinese medicine field. The invention relates to the Chinese Medicine Preparation for Treating Heat Stranguria, namely a new usage of the medicine compound with the patent No. of 02146487.1. The medicine compound is made from the following Chinese medical raw materials in a formula with a certain weigh proportion: 125 to 375 parts of honeysuckle, 62 to 187 parts of herba scutellariae barbatae, 37 to 112 parts of herba dianthi, 37 to 112 parts of knotgrass, 37 to 112 parts of folium pyrrosiae, 19 to 56 parts of fiveleaf akebia, 37 to 112 parts of semen plantaginis, 37 to 112 of parts common lophatherum herb, 12 to 37 parts of rush and 62 to 187 parts of mistletoe. The invention can be used for treating prostatitis, and the Chinese native medicines in the formula is rich in source, low in cost, convenient in carrying and easy to be accepted by users, so the medicine compound is applicable for the sufferers and has a substantial social and economical benefit.
Description
Technical field
The invention belongs to technical field of Chinese medicines.
Background technology
Jilin Huakang Pharmaceutical Co., Ltd has Chinese invention patent " Chinese medicine preparation of treatment pyretic stranguria ", the patent No. is 02146487.1, Granted publication CN1168488C, it is characterized in that, make by following Chinese medicine raw materials by weight proportion for per 1000 doses, 125～375 parts of Flos Loniceraes, 62～187 parts of Herba Scutellariae Barbataes, 37～112 parts of Herba Dianthis, 37～112 parts of Herba Polygoni Avicularis, 37～112 parts of Folium Pyrrosiaes, 19～56 parts of Caulis Akebiaes, 37～112 parts of Semen Plantaginiss, 37～112 parts of Herba Lophatheris, 12～37 parts of Medulla Juncies, 62～187 parts of Herba Taxillis.Its function of preparation of the present invention cures mainly to: heat-clearing and toxic substances removing, dampness removing is treating stranguria.Be applicable to that pyretic stranguria (syndrome of dampness-heat in lower jiao) is equivalent to each disease of acute urinary system infection.
Summary of the invention
The invention provides the application of a kind of pharmaceutical composition in the medicine of preparation resistance prostatitis, be " Chinese medicine preparation of treatment pyretic stranguria ", the patent No. is the new medicinal usage of 02146487.1 pharmaceutical composition, this pharmaceutical composition is made by following Chinese medicine raw materials by weight proportion, 125～375 parts of Flos Loniceraes, 62～187 parts of Herba Scutellariae Barbataes, 37～112 parts of Herba Dianthis, 37～112 parts of Herba Polygoni Avicularis, 37～112 parts of Folium Pyrrosiaes, 19～56 parts of Caulis Akebiaes, 37～112 parts of Semen Plantaginiss, 37～112 parts of Herba Lophatheris, 12～37 parts of Medulla Juncies, 62～187 parts of Herba Taxillis.
Among the present invention, this pharmaceutical composition is made by following Chinese medicine raw materials by weight proportion: 187～312 parts of Flos Loniceraes, 93～156 parts of Herba Scutellariae Barbataes, 56～93 parts of Herba Dianthis, 56～93 parts of Herba Polygoni Avicularis, 56～93 parts of Folium Pyrrosiaes, 28～47 parts of Caulis Akebiaes, 56～93 parts of Semen Plantaginiss, 56～93 parts of Herba Lophatheris, 19～31 parts of Medulla Juncies, 93～156 parts of Herba Taxillis.
Among the present invention, this pharmaceutical composition is made by following Chinese medicine raw materials by weight proportion: 190～270 parts of Flos Loniceraes, 110～136 parts of Herba Scutellariae Barbataes, 65～85 parts of Herba Dianthis, 65～85 parts of Herba Polygoni Avicularis, 65～85 parts of Folium Pyrrosiaes, 65～85 parts of Caulis Akebiaes, 65～85 parts of Semen Plantaginiss, 65～85 parts of Herba Lophatheris, 12～28 parts of Medulla Juncies, 110～136 parts of Herba Taxillis.
Above medical material weight portion if be that unit calculates and can be made into 1000 doses of medicaments with the gram, as is made 1000 of capsule preparations, 1000 in tablet etc.
Chinese medicine preparation of the present invention, can add the medicine acceptable carrier when being prepared into medicament, described medicine acceptable carrier can be: starch, sucrose, lactose, mannitol, silicon derivative, cellulose family and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, soil temperature 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Chinese medicine preparation of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, peroral dosage form preferably, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum, most preferably capsule.
The present invention has the effect of resistance prostatitis, the Chinese medicine in the pharmaceutical formulation, and its medicine source is abundant, and is cheap, easy to carry, easily accepted by the patient.Be applicable to the extensive patients use, so appreciable social benefit and economic benefit are arranged.Preparation of the present invention is determined usage and dosage according to patient's situation in use, but obeys every day three times, each 1～10/or sheet.
Chinese medicine preparation of the present invention can prepare by the following method:
Half amount of Flos Lonicerae is used ethanol extraction, filters, and reclaims ethanol, and is concentrated standby, and other gets 9 flavors such as Herba Scutellariae Barbatae, Herba Dianthi and decocts with water filtration, concentrates standby.Half amount, decontamination, the coarse pulverization of extracting honeysuckle again, with above two standby thick pastes, merging, drying is ground into fine powder, sieves, and obtains active component, and this active component can directly be made preparation, also can add the medicine acceptable carrier and make pharmaceutical preparation.
Further verify the present invention below by pharmacodynamic experiment.
Be subjected to the reagent thing
1. medicine of the present invention: hereinafter to be referred as: Flos Lonicerae is secreted scorching clever sheet (Yinhuamiyanling Pian), is provided lot number by Jilin Huakang Pharmaceutical Co., Ltd: 050903.Every crude drug content is 1.875g, and high performance liquid chromatography is carried out quantitative assay, and every of this product contains Flos Lonicerae, with chlorogenic acid (C
10H
18O
9) must not count and be less than 3.60mg.
2. compound method: face the time spent and Flos Lonicerae is secreted scorching clever sheet go to be made into desired concn with distilled water behind the film-coat, the time spent shakes up, 4 ℃ of preservations.
3. dosage is selected: oral 16 of everyone every day of being grown up, every heavy 0.5g, every contains crude drug in whole 1.875g, the people presses 70kg and calculates, be equivalent to 0.428g crude drug/kg, press human body and animal body surface area equivalent dose ratio and calculate, selecting three kinds of dosages of white mice is 15.0g, 7.5g, 3.75g crude drug/kg; Rat is 10.8g, 5.4g, 2.7g crude drug/kg (being equivalent to 4 times of dose,equivalent, 2 times and dose,equivalent respectively), the equal gastric infusion of animal (ig).Route of administration is with clinical consistent.
Animal
1.Wistar rat, closed colony, male and female dual-purpose, animal quality certification SCXK (Ji) 2003--0004.
2. outbreeding system, Kunming kind white mice, closed colony, body weight 18-22g, the animal quality certification number: SCXK (lucky 2003-0004.
Wistar rat and Kunming kind white mice provide by Changchun High-technology Medical Animal Experiment Research Center.
The raising condition
Animal housing is a regular grade, 26 ℃ of room temperatures, and relative humidity is 40%, the well-ventilated adopts standard feed to raise, white mice cage tool 26 * 16cm, male and female are divided cage, 5 in every cage.DABAI mouse cage tool 50 * 35cm, male and female are divided cage, 5 animals of every cage
The positive control medicine is selected
1. aspirin (enteric coatel tablets), clinical adult's every day oral this product 40mg, contain aspirin 25mg, face the time spent grinding, select dosage rat 0.2g/kg, white mice 0.3g/kg (determining) with reference to the interrelated data test dose, Shijiazhuang Shineway Pharmaceutical Co., Ltd produces, lot number: 20050209.
2 FUSAIMI PIAN, four of clinical adult every days, every contains furosemide 20mg.Tianjin Lisheng Pharmaceutical Co., Ltd. produces, and presses human body and animal body surface area equivalent dose ratio and calculates.Select dosage rat 36mg/kg (5 times of dose,equivalents), lot number: 040811.
3 QIANLIETONG PIAN, it is basic identical that (the accurate word Z44022456 of traditional Chinese medicines number) and Flos Lonicerae are secreted scorching clever sheet therapeutical effect, and the route of administration unanimity is so be chosen as positive reference substance.This product is a coated tablet, removes to show light brown to brown behind the sugar-coat the heavy 0.34g of substrate, per day for adults 3 times, is pressed human body and animal body surface area equivalent dose ratio and is calculated by each 6, selecting rat dosage is 2.75g/kg, be equivalent to 5 times of dose,equivalents, face time spent desaccharide clothing, grind, be made into desired concn with distilled water, animal is all irritated stomach (ig) administration, is produced lot number: 04507 by Guangzhou Zhongyi Medicine Industry Co., Ltd.
4 amoxicillin, everyone 2000mg every day that is grown up, 2 times of dose,equivalent 520mg/kg of test and Selection, Heilungkiang three diligent pharmaceutical Co. Ltds produce lot number: 031010
Instrument
1, JN one A type precision torsion balance (Shanghai Second Balance Factory)
2, SARTORIUS GMBH GOTTINGEN precision balance (precision: 0.00001), Germany
3, L-301 type biological microscope (optical instrument factory, Guangzhou)
4, ultramicrotome LKB2088 (Sweden's manufacturing)
5Leitj1512 paraffin slicing machine (Germany makes)
6SW-CJ-113 standard type clean work station (Suzhou Decontamination Equipment Plant)
7WX-753 type microcirculation microscope (Xuzhou medical optical instrument factory)
8 biomedical image analyzers (CIMA-400)
The multiple star FORTUNE.IMS microbial identification in 9 Shanghai/susceptibility analytical system
10, constant-temperature shaking culture case, HZQ-X100, Taicang experimental facilities factory
11 double superclean benches, SW-CT-ZF type, Chinese Suzhou
12 electric drying oven with forced convections, 101A-2 type, Chinese Shanghai;
13 vacuum freeze driers, MRK, Japan;
14 domestic cold storage refrigerators, BCD-226 type, U.S. Pedicellus et Pericarpium Trapae, Chinese Hefei.
Reagent:
Carrageenin: provide by Shenyang Pharmacy College's department of Chinese materia medica, face the time spent and prepare, no lot number with normal saline; Glacial acetic acid, produce lot number by Beijing northization fine chemical company limited: 020730,25% XIAOZHILING ZHUSHEYE, produce lot number: H0031 by Guangzhou traditional Chinese medical science pharmaceutcal corporation, Ltd; Sodium chloride (analytical pure), the Beijing Chemical Plant produces; Bacto Tryptone, OXOID, Britain; Bacto Yeast Extract, OXOID, Britain; Agar powder, Shanghai chemical reagent purchasing and supply station chemical reagent work produces, lot number: 77-07-14.Albumen, the East Sea, Shanghai pharmaceutical factory produces.
Bacterial strain: reference culture (American Type Culture Collection ATCC): colon bacillus ATCC44102, staphylococcus aureus ATCC26003, shigella flexneri ATCC51571.Identify that by Chinese pharmaceutical biological product institute provides.
Clinical isolates strain (Clinical Isolated Bacterial Strain CIBS): staphylococcus aureus strain CIBS831, staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, deformed rod bacterial strain CIBS1072, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, fungus Candida albicans strain CIBS850 and 3 strain coli strain CIBS872, CIBS614, CIBS853.Staphylococcus aureus, bacterium number: 26003 (Attached Hospital, Changchun Chinese Medicinal College's Bacteriology Room provides).
Data statistical approach:
The test 1～10 result data all with
The t check is adopted in expression, the significance of group difference, and test 10,11 results adopt χ
2Check.
Test method and result:
1, Flos Lonicerae is secreted scorching clever sheet activates thing chronic prostatitis model to xiaozhiling injection influence
Make the nonbacterial prostatitis rat model according to prostate direct injections such as Zhang Shi.Get 50 of Wistar rat, male, body weight 350-400g, 3% chloral hydrate 10ml/kg anaesthetizes through lumbar injection, under aseptic condition, the lower abdomen center disinfects the back in alcohol and mentions skin with tweezers, successively cuts skin, through abdominal cavity, mention bladder and both sides spermatic cord, expose bladder dorsal part prostate dorsal part leaf, inject 25% XIAOZHILING ZHUSHEYE 0.1ml respectively, suture muscles, skin, the iodine disinfection wound.Postoperative was raised 7 days meticulously, in the 7th day rat is divided into 5 groups at random, the 1st, 2,3 groups is that Flos Lonicerae is secreted scorching clever sheet height, in, low dose group, dosage is respectively 10.8g, 5.4g, 2.7g crude drug/kg, the 4th group is QIANLIETONG PIAN positive drug group, and dosage is 2.75g/kg, and the 5th group is model group, other gets 10 and is the normal control group, 1-4 organizes gastric infusion (ig) according to dosage, and all the other give distilled water, successive administration 30 days, in the last administration after 1 hour, weigh, the sacrificed by decapitation rat, dissection is got prostate and is weighed, respectively get then prostatic half, place the 1ml normal saline, fully mixing is measured leukocyte count and lecithin number in the prostatic fluid in microscopically, all the other prostate are fixed with 10% formalin, do histopathology section procuratorial work.The results are shown in Table 1, table 2.
Table 1 Flos Lonicerae is secreted the influence of scorching clever sheet to rat chronic prostatitis model prostatic fluid
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | Leukocyte count (individual/mg) | The lecithin number (individual/mg) |
| Flos Lonicerae is secreted scorching clever sheet QIANLIETONG PIAN model contrast normal control | 10.8 5.4 2.7 2.75 | 10 10 10 10 10 10 | 22.1±14.4 * 25.4±12.3 * 33.0±24.5 31.9±12.0 37.8±10.4 9.0±4.04### | 762.8±381.6 * 716.1±290.7 * 651.0±323.3 705.7±236.8 * 467.8±196.5 1245.1±751.8## |
Medication group and model control group be * P＜0.05 relatively
Normal control group and model group be ##P＜0.01###P＜0.001 relatively
Prostatic fluid is measured and is shown, model group and normal control group are relatively, leukocyte count obviously raises, and the lecithin number obviously reduces, confirm this model establishment, be subjected to reagent thing Flos Lonicerae to secrete the quantity of leucocyte and the apparent in view minimizing of model control group of scorching clever sheet height, middle dosage group, the lecithin number has obvious rising, and learning by statistics to handle has significant difference P＜0.05.
Table 2 Flos Lonicerae is secreted scorching clever sheet rat chronic prostatitis model is influenced the sxemiquantitative classification
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | 0 grade | 1 grade | 2 grades | 3 grades |
| Flos Lonicerae is secreted scorching clever sheet QIANLIETONG PIAN model contrast normal control | 10.8 5.4 2.7 2.75 | 10 10 10 10 10 10 | 7 6 3 6 0 10 | 3 3 6 4 0 0 | 0 1 1 0 4 0 | 0 0 0 0 6 0 |
Annotate: grade scale:
0 grade: normal prostatic, a matter does not have broadening and edema, and no cell infiltration does not have obvious proliferation of fibrous tissue;
1 grade: body of gland is arranged still neat, a light weight degree broadening, and edema is slighter, and a small amount of cell infiltration and proliferation of fibrous tissue are arranged;
2 grades: body of gland is arranged more neat, a matter moderate broadening, and edema is heavier, and more cell infiltration and proliferation of fibrous tissue are arranged;
3 grades: gland tissue has destruction, the obvious broadening of a matter, and edema is obvious, and visible a large amount of cell infiltration and obviously proliferation of fibrous tissue have the inflammatory cell of infiltration under the tunicle.
The histopathology observed result:
(1), normal control group: prostate lumen of gland out-of-shape, the glandular epithelium lumen of gland is projecting inward, forms a plurality of pleats and stretches to lumen of gland.Glandular epithelium is column or cube, and epithelial proliferation is inactive, and the nipple of formation is few and lack, visible thin Yihong secretions of having a liking in the lumen of gland.Closely arrange between body of gland, a matter is narrow, and a matter composition obviously is less than body of gland, and a matter includes and a small amount of smooth muscle cell, lymphocyte and fibroblast.Be about 1/3 of body of gland, do not have obvious inflammatory cell.And marshalling,
(2), model group: body of prostate is arranged loose, and glandular epithelium is column.Glandular epithelium does not have obvious hypertrophy, and a matter is obviously widened, edema, visible most lymphocytic infiltrations and fibroblast proliferation.The normal matched group of deposition of fibrous tissue is many.The normal matched group showed increased of some position deposition of fibrous tissue.
(3), positive control drug group: body of gland is arranged more loose, and glandular epithelium is shorter, and glandular epithelium does not have obvious hypertrophy, does not form more prominent nipple to lumen of gland.As seen have a liking for Yihong secretions in the lumen of gland on a small quantity.Closely arrange between body of gland.Between the light weight degree widen, the degree of lymphocytic infiltration and fibroblast proliferation obviously alleviates than model group, the interstitial fibers tissue deposition is not obvious.
(4), high dose group: body of gland is arranged and is tending towards the form of normal control group, and glandular epithelium is column or cube, and adenomatosis is not obvious, does not form most prominent nipples to lumen of gland.As seen have a liking for Yihong secretions in the lumen of gland on a small quantity.Compare with model group, a matter width obviously narrows down, and edema obviously alleviates, and the inflammatory cell of infiltration is sparse.The interstitial fibers tissue deposition is not obvious.
(5), middle dosage group: glandular epithelium does not have obvious hypertrophy, does not form more prominent mastoid process to lumen of gland, as seen has a liking for Yihong secretions in the lumen of gland on a small quantity.Body of gland form and positive controls are close, and a matter widens degree and interstitial edema obviously alleviates, and the inflammatory cell quantity of infiltration reduces, sparse distribution, and the interstitial fibers tissue deposition is not obvious.
(6), low dose group: body of gland is arranged still neat, and glandular epithelium does not have obvious hypertrophy, does not form more prominent mastoid process to lumen of gland, as seen has a liking for Yihong secretions in the lumen of gland on a small quantity.Between matter widen with edema and alleviate to some extent than model group, a matter inflammatory cell quantity reduces, visible a small amount of interstitial fibers tissue deposition.
Conclusion:
The model group body of gland is arranged more loose, disorder, and the obvious edema of a matter is widened, as seen most cell infiltration, fibroblast proliferation and deposition of fibrous tissue occur, some position deposition of fibrous tissue showed increased shows that the chronic prostatitis model of foundation is successful.
Flos Lonicerae is secreted scorching clever sheet height, middle dosage group and positive drug control group, the prostate body of gland is arranged and is tending towards normal, matter is widened between prostate, the edema degree obviously alleviates than model group, between the inflammatory cell that soaks into of matter obviously reduce, the interstitial fibers tissue deposition obviously alleviates, and wherein the improvement effect of high dose is the most obvious.
2 Flos Lonicerae are secreted the influence of scorching clever sheet to the rat nonbacterial prostatitis
Get 60 of rats, body weight 300-350g, after be divided into 6 groups at random, 1-3 group is secreted the high, medium and low dosage group of scorching clever sheet for Flos Lonicerae, dosage is respectively 10.8g, 5.4g, 2.7g crude drug/kg, 4 groups of positive medicine QIANLIETONG PIAN groups, dosage is 2.75g/kg, and 5 groups is model control group, and 6 groups is the normal control group, the 1-4 group is all irritated stomach (ig) administration, and model control group and normal control group give with the volume distilled water by the administration high dose.Every day 1 time, continuous 10 days, 1h improves a little by the Dai Shi method after the last administration, every Mus is cut the abdominal cavity with lumbar injection 3% chloral hydrate 10ml/kg anesthesia open under the aseptic condition, injects sterilization 1.5% carrageenin 0.05ml in every Mus prostate, (normal control group injection physiological saline solution), sew up 24h sacrificed by decapitation rat, (row is administered once again) therebetween, getting prostate weighs, after get half prostate in the 1ml normal saline, fully mixing is drawn the prostate suspension with capillary tube, splash in the blood countng chamber, move into microscopically,, press record leukocyte count and Lecithin corpusculum number under the leukocyte method mirror with the low power lens counting; Simultaneously prostatic fluid is inoculated on the common blood agar plate, cultivates 24h for 37 ℃.Inspection has or not colony growth.Residue prostate formalin fixed is got rat prostate and is done histopathologic examination.The results are shown in Table 3, table 4.
The influence of table 3 pair rat nonbacterial prostatitis model prostatic fluid test rating
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | Prostate coefficient (g/100g) | Leukocyte count (individual/mg) | The lecithin number (individual/mg) |
| Flos Lonicerae is secreted scorching clever sheet QIANLIETONG PIAN model contrast normal control | 10.8 5.4 2.7 2.75 | 10 10 10 10 10 10 | 0.15±0.047 * 0.16±0.056 0.19±0.079 0.17±0.061 0.20±0.041 0.15±0.018## | 18.4±10.67 *17.6±10.45 *21.7±13.216 19.2±11.52 *33.0±16.09 9.76±3.99### | 794.4±331.7 **829.8±472.7 *632.1±181.6 *773.1±299.6 **437.8±149.5 987.1±231.3### |
Medication group and model control group are relatively
*P＜0.05
*P＜0.01
Normal control group and model group are relatively
*P＜0.01
* *P＜0.001
The result shows by table 3, Flos Lonicerae is secreted scorching clever sheet 10.8g crude drug/kg, 5.4g crude drug/kg dosage group and rat carrageenan prostatitis model is had the obvious suppression leucocyte hyperplasia and improve the effect of Lecithin corpusculum density, relatively distinguishes P＜0.01, P＜0.05 with model control group.Prostatic fluid is cultivated and be there is no colony growth simultaneously.
Pathological study result shows, the normal control group: the prostate lumen of gland is regular, closely arranges between body of gland, and a matter is narrow, does not have obvious cell infiltration.Model control group: body of prostate is arranged loose, disorder, and a matter is obviously widened, edema, visible most lymphocytic infiltrations and fibroblast proliferation.The normal matched group of deposition of fibrous tissue is many.Cell infiltration obvious (success of prostatitis model) under the visible capsule of prostate in some position.Flos Lonicerae is secreted scorching clever sheet 10.8g crude drug/kg dosage group: body of gland is arranged and is tending towards normal control group degree, and adenomatosis is not obvious.Between the matter width than the obvious constriction of model group, edema is slight, the inflammatory cell of infiltration is sparse.Most of tunicle is not seen obvious cell infiltration.Flos Lonicerae is secreted scorching clever sheet 5.4g crude drug/kg dosage group: body of gland is arranged more loose, and the matter degree of widening obviously alleviates, and the inflammatory cell quantity that a matter is soaked into reduces, and edema alleviates, visible minority cell infiltration under the capsule of prostate.Positive drug QIANLIETONG PIAN group: body of gland form and Flos Lonicerae secrete that scorching clever sheet 5.4g crude drug/kg dosage group is close, and the matter degree of widening alleviates than model control group, the inflammatory cell negligible amounts that a matter is soaked into.In sum, Flos Lonicerae is secreted scorching clever sheet to be had the rat prostate inflammation and obviously alleviates effect, especially remarkable with effect of high dosage.(with the histopathology photo).
Table 4 Flos Lonicerae is secreted scorching clever sheet rat nonbacterial prostatitis model is influenced the sxemiquantitative classification
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | 0 grade | 1 grade | 2 grades | 3 grades |
| Flos Lonicerae is secreted scorching clever sheet QIANLIETONG PIAN model contrast normal control | 10.8 5.4 2.7 2.75 | 10 10 10 10 10 10 | 7 6 2 6 0 10 | 3 4 8 4 1 0 | 0 0 0 0 4 0 | 0 0 0 0 5 0 |
Annotate: grade scale: (the sxemiquantitative grade scale sees Table 2 grade scales)
The result shows by table 4, and Flos Lonicerae is secreted scorching clever sheet height, middle dosage group all has the effect that alleviates the prostata tissue pathological changes, and the effect of wherein administration high dose group is the most obvious.
3, Flos Lonicerae is secreted the influence of scorching clever sheet to rat bacterial prostatitis due to the escherichia coli
Get 60 of rats, body weight 280-340g, after be divided into six groups at random, press the Dai Shi method with 0.1ml colibacillus (1.4 * 10
7/ ml) cause inflammation (method is with 2), cause scorching preceding 5 days and begin by group shown in the table 4 and dosage grouping administration (ig), model group and normal control group give with the volume distilled water by the administration high dose.Cause scorching back and continue administration 3 days every day 1 time, 1h after the last administration, and the sacrificed by decapitation rat is got prostate 50mg respectively under aseptic condition, in the 1ml normal saline, abundant mixing, mirror is record leukocyte count and Lecithin corpusculum number down; Simultaneously prostatic fluid is inoculated on the common blood agar plate, cultivates 48h for 37 ℃.The record clump count, each group is got prostate and is done histopathologic examination with formalin fixed simultaneously.
Table 5 Flos Lonicerae is secreted scorching clever sheet causes rat bacterial prostatitis model prostatic fluid test rating to escherichia coli influence
| Group and dosage (the g crude drug/kg) | Number of animals (only) | Leukocyte count (individual/mg) | Lecithin corpusculum number (individual/mg) | Clump count (individual) |
| Flos Lonicerae is secreted scorching clever sheet 10.8 5.4 2.7 QIANLIETONG PIAN 2.75g/kg models contrast (distilled water) normal control (distilled water) | 10 10 10 10 10 10 | 6.61±1.90* 6.32±2.24* 7.72±1.21 6.81±1.97* 37.0±9.49 1.73±0.46*** | 243±53.02* 243±77.80* 214±22.90 244±53.25* 183±43.17 445±66.94*** | 4.7±1.70* 4.9±1.73 5.7±1.42 4.7±1.49* 6.6±1.95 0.7±0.82*** |
Administration group and model control group are relatively
*P＜0.05
*P＜0.01
Normal control group and model control group are relatively
*P＜0.01
* *P＜0.001
The result shows by table 5, Flos Lonicerae is secreted scorching clever sheet 10.8g crude drug/kg, 5.4g crude drug/kg dosage group causes rat bacterial prostatitis model to escherichia coli and has tangible minimizing quantity of leucocyte and improve the effect of Lecithin corpusculum density, also can reduce the bacterial population in the prostatitis rat prostate simultaneously, with model control group statistical significance is arranged more all, P＜0.01, P＜0.05.
4, the influence that the intraperitoneal mouse capillary permeability is increased
Choose 50 of healthy mices, body weight is 18-22g, and male and female half and half are divided into 5 groups at random, 10 of every treated animals.The 1st, 2,3 groups is that Flos Lonicerae is secreted scorching clever sheet height, in, low dose group, dosage is 15.0g respectively, 7.5g, 3.75g crude drug/kg, the 4th group of positive medicine aspirin, dosage is 0.3g/kg, the 5th group is matched group, the continuous gastric infusion of animal 5 days, tail vein injection 0.5% azovan blue solution 0.01mL/g behind last administration 1h, lumbar injection 0.6% acetic acid normal saline 0.2ml/ only takes off cervical vertebra and puts to death white mice behind the 20min immediately, dabs abdominal part 1min, cutting the abdominal cavity open washes repeatedly with an amount of normal saline, collect eluate, filter paper filters, and adjusts volume to 6mL with normal saline, with the centrifugal 15min of centrifuge (3000rPm), get supernatant (wavelength 590nm) in 752 type spectrophotometers and measure OD value, each difference of organizing relatively, and directly calculate suppression ratio.The results are shown in Table 6.
Table 6 Flos Lonicerae is secreted scorching clever sheet to the influence of intraperitoneal mouse capillary permeability (X ± SD)
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | The OD value |
| Flos Lonicerae is secreted scorching clever sheet aspirin contrast | 15.0 7.5 3.75 0.3 | 10 10 10 10 10 | 0.488±0.088 * 0.462±0.216 * 0.533±0.189 0.466±0.093 ** 0.657±0.174 |
Compare with matched group
*P＜0.05
*P＜0.01
Table 6 result shows, Flos Lonicerae secrete scorching clever sheet height, middle dosage Dichlorodiphenyl Acetate cause the intraperitoneal mouse capillary permeability increase and have obvious inhibitory action (with matched group relatively, P＜0.05), but its effect is not as positive drug (P＜0.01)
5, to the influence of rat thoracic cavity leukoplania
Get 50 of rat, male and female half and half are divided into 5 groups at random, 10 of every treated animals, the 1-3 group is secreted the high, medium and low dosage group of scorching clever sheet for Flos Lonicerae, and dosage is respectively 10.8g, 5.4g, 2.7g/kg, 4 groups of positive medicine aspirin groups, dosage is 0.2g/kg, 5 groups is matched group, and the 1-4 group is according to dosage irritated stomach (ig) administration, every day 1 time during experiment, continuous 5 days, matched group gave with the volume distilled water by the administration high dose group.Behind last administration 1h, rat is used the ether light anaesthesia, face upward the position and be fixed on the operating-table.Inject 0.5% carrageenin 0.5ml in thoracic cavity, rat right side, sacrificed by decapitation rat after 5 hours is opened the thoracic cavity at the tabula periphery, and with syringe sucking-off pleural fluid, record seepage discharge and leukocyte count the results are shown in Table 7.
Table 7 Flos Lonicerae is secreted scorching clever sheet to the influence of rat thoracic cavity leukoplania (X ± SD)
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | Transudate (ml) | Leukocyte count (* 10 7Individual/ml) |
| Flos Lonicerae is secreted scorching clever sheet aspirin contrast | 10.8 5.4 2.7 0.2 | 10 10 10 10 10 | 1.69±0.39 *1.90±0.41 1.85±0.31 1.66±0.35 **2.13±0.37 | 3.59±1.36 * 4.32±2.70 ** 4.44±0.99 3.39±0.88 ** 5.57±1.84 |
Compare with matched group
*P＜0.05
*P＜0.01
As shown in Table 7, Flos Lonicerae is secreted scorching clever sheet height, the equal rat of middle dosage thoracic cavity leukoplania has certain inhibitory action, but its effect is weaker than positive control drug aspirin.
6, to the influence of rat chondrus ocellatus Holmes colloidality foot swelling
Get 50 of rat, male, be divided into five groups at random, 10 of every treated animals, the 1-3 group is secreted the high, medium and low dosage group of scorching clever sheet for Flos Lonicerae, and dosage is respectively 10.8g, 5.4g, 2.7g/kg, 4 groups of positive medicine aspirin groups, dosage is 0.2g/kg, 5 groups is matched group, and the 1-4 group is according to dosage irritated stomach (ig) administration, every day 1 time during experiment, continuous 7 days, matched group gave with the volume distilled water by the administration high dose group.Measure the left back ankle of rat joint girth with self-control arrowband chi.Behind last administration 30min, respectively only at the right back sufficient subcutaneous injection 1% carrageenin suspension 0.1ml/ of rat.Per hour survey right back ankle joint girth 1 time, altogether 6h.From as contrast, is inflammation swelling degree (mm) with the difference of left and right sides metapedes ankle joint girth with the left back foot of every Mus, the results are shown in Table 8
Table 8 Flos Lonicerae is secreted scorching clever sheet to the influence of rat chondrus ocellatus Holmes colloidality foot swelling (X ± SD)
| Group | Dosage g/kg | Number of animals (only) | Cause scorching back different time 1h | (h) foot swelling degree (mm) 2h | (X±SD) 3h |
| Flos Lonicerae is secreted scorching clever sheet aspirin contrast (distilled water) | 10.8 5.4 2.7 0.2 | 10 10 10 10 10 | 3.75±0.82 ** 3.95±0.93 * 3.80±1.00 * 3.20±0.82 *** 4.70±0.42 | 3.05±1.48 ***3.45±1.71 **4.50±0.78 *2.45±0.90 ***5.45±0.90 | 3.40±0.94 **4.05±1.44 4.90±1.29 2.70±0.42 ***5.00±1.13 |
| Group | Dosage g/kg | Number of animals (only) | Cause scorching back different time 4h | (h) foot swelling degree (mm) 5h | (X±SD) 6h |
| Flos Lonicerae is secreted scorching clever sheet aspirin contrast (distilled water) | 10.8 5.4 2.7 0.2 | 10 10 10 10 10 | 3.50±1.68 ** 5.15±1.76 5.65±1.23 2.40±0.52 *** 5.75±1.11 | 3.65±1.84 *5.05±1.28 5.55±1.14 2.90±0.66 ***5.40±1.88 | 4.50±1.55 5.25±0.86 5.30±1.06 3.05±0.60 ***5.50±0.82 |
Compare with matched group
*P＜0.05
*P＜0.01
* *P＜0.001
The result shows by table 8, and Flos Lonicerae is secreted scorching clever sheet 10.8g/kg group, and foot swelling has the obvious suppression effect to 1～5h rat chondrus ocellatus Holmes colloidality; Flos Lonicerae secretes scorching clever sheet 10.8g crude drug/kg group and the 5.4g crude drug/kg group 1～2h has inhibitory action, but its effect illustrates that not as the positive drug aspirin this product has certain antiinflammatory action.
7, to the influence of white mice writhing response incidence rate
Get 50 of white mice, male and female half and half are divided into five groups at random by dosage shown in the table 9,10 of every treated animals, animal ig administration, every day 1 time, continuous 5 days (positive drug administration on the same day), matched group is given with the volume distilled water, 1h after the last administration, every Mus is begun behind the 5min to observe and the interior white mice writhing response number of times of record 10min by lumbar injection 0.6% acetum 0.1ml/10g (body weight), the results are shown in Table 9.
Computing formula:
Table 9 Flos Lonicerae is secreted scorching clever sheet to the influence of white mice writhing response (X ± SD)
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | White mice is turned round body number (inferior) | Suppression ratio (%) |
| Flos Lonicerae is secreted scorching clever sheet aspirin contrast | 15.0 7.50 3.75 0.3 | 10 10 10 10 10 | 16.5±6.59 * 16.6±5.95 ** 19.4±8.68 14.9±5.72 *** 23.3±5.21 | 29.18 28.75 16.74 36.05 |
Compare * * * P＜0.001 with matched group
The result shows by table 9, and Flos Lonicerae is secreted high, medium and low three dosage of scorching clever sheet and all had significant analgesia role, and suppression ratio is respectively 47.21%, 42.49%, 51.07%.But effect is not as positive drug aspirin group.
8, to the diuresis of rat
Get 50 of rat, be divided into 5 groups at random, the 1st, 2,3 group is that Flos Lonicerae is secreted the high, medium and low dosage group of scorching clever sheet, the 4th group of positive medicine FUSAIMI PIAN group, and the 5th group is matched group.Each administration group is gastric infusion according to dosage, continuous 5 days, matched group is given with the volume distilled water, after the last administration 0.5 hour, each Mus was pressed the normal saline lumbar injection of 2.5ml/100g, and gently depressed abdominal part and make bladder emptying (test fasting the previous day is water 18hr not only), immediately rat is put into metabolic cage, a cage is put 1 of animal, collects the urine amount totally 3 times of each treated animal after 60 minutes, each 60 minutes.Relatively, take statistics to learn and handle between the average of each the group urine amount of rat in the corresponding time organized, the results are shown in Table 10.
Table 10 Flos Lonicerae is secreted scorching clever sheet to the influence of rat urine amount (X ± SD)
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | Different time urine amount (ml) after the administration | Total volume of urine (ml) | |
| 0～1h | 1～4h | ||||
| Flos Lonicerae is secreted scorching clever sheet furosemide contrast | 10.8 5.4 2.7 36mg/kg | 10 10 10 10 10 | 1.95±1.08 * 4.26±0.75 3.22±1.03 8.64±1.93 *** 3.44±1.64 | 4.05±1.52 2.74±1.10 2.28±0.94 5.28±1.05 ***2.93±1.29 | 9.00±1.06 *7.0±1.75 5.5±1.68 13.9±2.61 ***6.37±2.82 |
Compare with matched group
*P＜0.05
* *P＜0.001
By last table result show Flos Lonicerae secrete scorching clever sheet high dose group to the diuresis of white mice significantly (
*P＜0.05), but the effect not as the positive drug group (
* *P＜0.005), middle dosage group and low dose group action effect are not obvious.
9, Flos Lonicerae is secreted the influence of scorching clever sheet to rat granuloma hamartoplasia
Get 50 of rats, every Mus is with lumbar injection 3% chloral hydrate 10ml/kg anesthesia anesthesia, under aseptic condition, with strange portion iodine disinfection about rat, after 75% ethanol took off iodine, each cut the long osculum of 1-1.5cm, with the ophthalmology tweezer 20mg (sterilization) cotton balls is imbedded subcutaneous, skin suture immediately, animal was stablized one day, be divided into 5 at random, 10 every group, press dosage shown in the table 11, the equal ig administration of animal, every day 1 time, continuous 7 days, matched group was given with the volume distilled water.In the 7th day rat is put to death, open former otch, take out cotton balls, reject fatty tissue, put 70 ℃ of oven dry of baking oven, weigh.The results are shown in Table 11.
Computing formula:
Granuloma weight=(two granuloma weight/2)-20mg
Table 11 Flos Lonicerae is secreted the influence of scorching clever sheet to rat granuloma hamartoplasia
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | Granuloma induced by implantation of cotton pellets heavy (mg) | Suppression ratio (%) |
| Flos Lonicerae is secreted scorching clever sheet aspirin contrast | 10.8 5.4 2.7 0.2g/kg | 10 10 10 10 10 | 80.7±17.68 ** 87.1±18.80 * 101.2±35.66 74.1±8.76 *** 114.4±29.87 | 29.46 23.86 11.54 35.23 |
Compare * P＜0.05**P＜0.01***P＜0.001 with matched group
As shown in Table 11, Flos Lonicerae is secreted scorching clever sheet 10.8g crude drug/kg, two kinds of dosage of 5.4g crude drug/kg have the obvious suppression effect to cott on pellet-induced granuloma formation, wherein 10.8g crude drug/kg group is learned by statistics and is handled P＜0.01, but effect is not as positive control drug aspirin.
10, Flos Lonicerae is secreted scorching clever sheet to infecting the protective effect of white mice in the staphylococcus aureus body
With the staphylococcus aureus dried frozen aquatic products, be inoculated in the fluid medium after the recovery, in incubator, cultivated 18 hours for 37 ℃, take out the back and use normal saline, add the bacteria suspension that 5% concentration gastron (to strengthen virulence) is made different gradients simultaneously with 10 times of serial dilutions.Through trial test, lumbar injection staphylococcus aureus minimum lethal dose (MLD) is 10 under this experimental condition
-1Formal test, get 75 of mices, male and female half and half, body weight 18-20g, be divided into 5 groups at random, every group 15,1st, 2,3 groups is that Flos Lonicerae is secreted scorching clever sheet group, and dosage is respectively 31.2g, 15.6g, 7.8g crude drug/kg (be equivalent to respectively mice dose,equivalent 8 times, 4 times, 2 times), the 4th group of positive medicine Amoxicillin Capsules group, dosage is 0.52g/kg (being equivalent to 2 times of dose,equivalents), give distilled water 10ml/kg for the normal control group for the 5th group, every day, the ig administration was 2 times, the upper and lower noon each once, successive administration 5 days was in administration pneumoretroperitoneum injection 10 in the 5th day
-1The staphylococcus aureus suspension, every 0.5ml, and continue administration, and observe 24h dead animal number, calculate mortality rate and carry out x
2Statistical procedures the results are shown in Table 12.
Table 12 Flos Lonicerae is secreted scorching clever sheet to infecting mouse protective effect in the staphylococcus aureus body
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | Death toll (only) | Survival rate (%) | The P value |
| Flos Lonicerae is secreted the contrast of scorching clever sheet amoxicillin | 31.2 15.6 7.8 0.52 distilled water | 15 15 15 15 15 | 5 7 10 4 13 | 66.7 53.3 33.3 73.3 13.3 | ＜0.05 ＞0.05 ＞0.05 ＜0.01 |
Table 12 is the result show; Flos Lonicerae is secreted scorching clever sheet 31.2g crude drug/kg dosage group and staphylococcus aureus is caused in the mice body to infect significant protective effect and matched group comparison P＜0.05 are arranged; other two dosage also have inhibition trend in various degree, but statistical procedures does not have explicitly difference.Illustrate that this product has bacteriostasis in the tangible body.
11, Flos Lonicerae is secreted scorching clever sheet to infecting the protective effect of white mice in the escherichia coli body
With the escherichia coli dried frozen aquatic products, be inoculated in the fluid medium after the recovery, in incubator, cultivated 18 hours for 37 ℃, take out the back and use normal saline, add the bacteria suspension that 5% concentration gastron (to strengthen virulence) is made different gradients simultaneously with 10 times of serial dilutions.Through trial test, lumbar injection staphylococcus aureus minimum lethal dose (MLD) is a bacterium stock solution under this experimental condition.Formal test, get 75 of mices, male and female half and half, body weight 18-20g, be divided into 5 groups at random, every group 15,1st, 2,3 groups is that Flos Lonicerae is secreted scorching clever sheet group, and dosage is respectively 31.2g, 15.6g, 7.8g crude drug/kg (be equivalent to respectively mice dose,equivalent 8 times, 4 times, 2 times), the 4th group of positive medicine Amoxicillin Capsules group, dosage is 0.52g/kg (being equivalent to 2 times of dose,equivalents), give distilled water 10ml/kg for the normal control group for the 5th group, every day, the ig administration was 2 times, the upper and lower noon each once, successive administration 5 days was in administration pneumoretroperitoneum injection 10 in the 5th day
-1The staphylococcus aureus suspension, every 0.5ml, and continue administration, and observe 24h dead animal number, calculate mortality rate and carry out x
2Statistical procedures the results are shown in Table 13.
Table 13 Flos Lonicerae is secreted scorching clever sheet to infecting mouse protective effect in the escherichia coli body
| Group | Dosage (the g crude drug/kg) | Number of animals (only) | Death toll (only) | Survival rate (%) | The P value |
| Flos Lonicerae is secreted the contrast of scorching clever sheet amoxicillin | 31.2 15.6 7.8 0.52 distilled water | 15 15 15 15 15 | 4 7 11 4 14 | 73.3 53.3 36.7 73.3 6.7 | ＜0.05 ＞0.05 ＞0.05 ＜0.01 |
Table 13 is the result show; Flos Lonicerae is secreted scorching clever sheet 31.2g crude drug/kg dosage group and staphylococcus aureus is caused in the mice body to infect significant protective effect and matched group comparison P＜0.01 are arranged; other two dosage also have inhibition trend in various degree, but statistical procedures does not have explicitly difference.Illustrate that this product has bacteriostasis in the tangible body.
12, Flos Lonicerae is secreted external bacteriostatic test of scorching clever sheet and bactericidal assay
1. minimum inhibitory concentration (MIC) is measured: with Flos Lonicerae secrete scorching clever sheet medicated powder with the LB fluid medium be mixed with 300,250,200,150,100,80,60,40,20 respectively, 10mgml
-1Experiment is established 21 groups, and the 1-10 group adds above-mentioned 300mgml respectively
-1-10mgml
-1The medicinal liquid of 10 concentration, every group of 3 parallel control pipes; The 11-20 group does not add bacterium liquid group for only adding the clever sheet medicinal liquid of the secret inflammation of above-mentioned same concentrations Flos Lonicerae, as observing the medicine matched group whether medicine pollutes, every group of 3 parallel control pipes; Do not secrete the matched group that scorching clever sheet medicinal liquid only adds antibacterial for not adding Flos Lonicerae for 21 groups, establish 3 parallel control pipes simultaneously.To be coated with flat board behind the suitable multiple of inoculated bacteria dilution, overnight incubation in 37 ℃ of incubators, colony counting is also taken pictures.With each the group developmental tube put cultivate 16～18 hours in 37 ℃ of constant-temperature shaking culture casees after, to observe above each drug component of lowest concentration of drug of not seeing bacterial growth (comparing) and the suitable multiple of liquid in pipe dilution will not be coated with flat board, overnight incubation in 37 ℃ of incubators with the medicinal liquid matched group.Colony counting is also taken pictures.Bacteriostasis rate reaches 99.99% lowest concentration of drug and is minimum inhibitory concentration (MIC).Measurement result table 14 and table 15.
2. minimum bactericidal concentration (MBC) is measured: with Flos Lonicerae secrete scorching clever sheet medicated powder with the LB fluid medium be mixed with 300,250,200,150,100,80,60,40,20 respectively, 10mgml
-1Experiment is established 21 groups, and the 1-10 group adds above-mentioned 300mgml respectively
-1-10mgml
-1The medicinal liquid of 10 concentration, every group of 3 parallel control pipes; The 11-20 group does not add bacterium liquid group for only adding the clever sheet medicinal liquid of the secret inflammation of above-mentioned same concentrations Flos Lonicerae, as observing the medicine matched group whether medicine pollutes, every group of 3 parallel control pipes; Do not secrete the matched group that scorching clever sheet medicinal liquid only adds antibacterial for not adding Flos Lonicerae for 21 groups, establish 3 parallel control pipes simultaneously.Behind inoculation bacterium liquid, put in 37 ℃ of constant-temperature shaking culture casees and cultivated 16～18 hours.Observe each above developmental tube culture of medicine minimum inhibitory concentration (MIC), draw 0.1ml respectively, move on the ordinary flat agar culture medium, smoothen bacterium liquid with Glass rod, put 37 ℃ of overnight incubation, in the plating medium, counting is less than the minimum bactericidal concentration (MBC) of 5 bacterium colony persons as this medicine.Measurement result sees Table 15
3. result and analysis: patina is secreted scorching clever sheet to colon bacillus strain ATCC44102, staphylococcus aureus strain ATCC26003, shigella flexneri strain ATCC51571; Clinical SEPARATION OF GOLD Staphylococcus aureus strain CIBS831, staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, deformed rod bacterial strain CIBS1072, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, fungus Candida albicans strain CIBS850 and 3 strain coli strain CIBS872, the external bacteriostatic experiment of CIBS614, CIBS853 all have antibacterial preferably and bactericidal effect.Patina is secreted scorching clever sheet the MIC of coli strain CIBS872, CIBS614, CIBS853, staphylococcus epidermidis strain CIBS 2972, deformed rod bacterial strain CIBS 1072, enterococcus faecalis strain CIBS 859, colon bacillus strain ATCC44102, staphylococcus aureus strain ATCC26003 is 80mgml
-1The MIC of staphylococcus aureus strain CIBS 831 is 100mgml
-1The MIC of klebsiella spp strain CIBS 861 is 150mgml
-1The MIC of staphylococcus saprophyticus strain CIBS 3586 and bacillus pyocyaneus strain CIBS 868 is 60mgml
-1The MIC of fungus candida albicans strain CIBS 861 and shigella flexneri ATCC51571 is 40mgml
-1The MBC of staphylococcus aureus strain CIBS 831 and colon bacillus strain ATCC44102 is 300mgml
-1The MBC of coli strain CIBS872, deformed rod bacterial strain CIBS1072 and staphylococcus aureus ATCC26003 is 250mgml
-1The MBC of coli strain CIBS614, CIBS853, staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, fungus candida albicans strain CIBS861 is 200mgml
-1The MBC of shigella flexneri ATCC51571 is 150mgml
-1, the MBC of bacillus pyocyaneus strain CIBS868 is 80mgml
-1From top result as can be seen, Flos Lonicerae is secreted scorching clever sheet to e. coli strains CIBS872, CIBS614, CIBS853, and staphylococcus epidermidis strain CIBS2972, deformed rod bacterial strain CIBS1072, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, fungus candida albicans strain CIBS850 and colon bacillus strain ATCC44102, staphylococcus aureus strain ATCC26003, shigella flexneri strain ATCC51571 all have certain bacteriostasis; Flos Lonicerae is secreted scorching clever sheet to e. coli strains CIBS614, CIBS853, and staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, shigella flexneri strain ATCC5 1571 and fungus candida albicans strain CIBS861 all have certain bactericidal action.
Above-mentioned result of the test shows that Flos Lonicerae is secreted scorching clever sheet to reference culture antibacterial colon bacillus strain ATCC44102, staphylococcus aureus strain ATCC26003, shigella flexneri strain ATCC51571; Clinical SEPARATION OF GOLD Staphylococcus aureus strain CIBS831, staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, deformed rod bacterial strain CIBS1072, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, fungus Candida albicans strain CIBS850 and 3 strain coli strain CIBS872, CIBS614, CIBS853 have certain inhibitory action.
Table 14 Flos Lonicerae is secreted scorching clever sheet is cultivated bacterial strain to difference bacteriostasis rate (%) (unit: mgml
-1 N=3)
| Strain | 300 | 250 | 200 | 150 | 100 | 80 | 60 | 40 | 20 | 10 |
| Escherichia coli 872 escherichia coli 614 escherichia coli 853 gold medal Portugal bacterium 831 staphylococcus epidermidiss 2972 staphylococcus saprophyticus 3586 Bacillus proteuss 1072 klebsiella spps 861 enterococcus faecalis 859 bacillus pyocyaneus 868 Candida albicans 850 colon bacillus 44102 gold medal Portugal bacterium 26003 shigella flexneris 51571 | 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 | 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 | 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 | 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 99.98±0.23 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 | 100.00±0.0 100.00±0.0 100.00±0.0 99..97±0.67 100.00±0.0 100.00±0.0 100.00±0.0 78..98±0.83 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 100.00±0.0 | 99.99±0.35 99.98±0.23 99..99±0.78 69..67±0.67 98..88±0.77 100.00±0.0 99..96±0.67 54..88±0.49 99.96±0.10 100.00±0.0 100.00±0.0 99.96±0.24 99.76±0.78 100.00±0.0 | 75.19±0.55 73.42±0.67 77..89±0.65 40.56±0.47 70..87±0.54 99.99±0.35 79.45±0.29 27..95±0.66 70.98±0.22 99.78±0.33 100.00±0.0 70..05±0.32 71.07±0.10 100.00±0.0 | 30.17±0.67 31.47±0.25 33.76±0.89 15.87±0.95 36.98±0.33 30.76±0.12 35.91±0.22 11..91±0.77 39.98±0.70 40.76±0.12 99.90±0.65 37.98±0.54 36.76±0.21 98.67±0.33 | 5.98±0.34 6.19±0.14 5.90±0.11 4.87±0.67 6.87±0.80 6.29±0.71 6.98±0.93 1.10±0.19 6.09±0.11 5.98±0.91 46.00±0.17 6.49±0.87 6.32±0.32 35.09±0.90 | 1.43±0.15 1.25±0.57 1.54±0.98 0.99±0.11 1.98±0.91 0.99±0.32 1.98±0.33 0.36±0.29 2.00±1.09 1.90±0.12 11.95±0.09 2.09±0.23 1.94±0.56 11.09±0.01 |
Table 15 Flos Lonicerae is secreted scorching clever sheet to In vitro culture different strains bacteriostatic experiment MIC and the MBC result of calculation (mgml of unit
-1)
| Bacterial strain | Escherichia coli 872 | Escherichia coli 614 | Escherichia coli 853 | Staphylococcus aureus 831 | Staphylococcus epidermidis 2972 | Staphylococcus saprophyticus 3586 | Bacillus proteus 1072 | Klebsiella spp 861 | Enterococcus faecalis 859 | Bacillus pyocyaneus 868 | Candida albicans 850 | Colon bacillus 44102 | Staphylococcus aureus 26003 | Shigella flexneri 51571 |
| MIC | 80 | 80 | 80 | 100 | 80 | 60 | 80 | 150 | 80 | 60 | 40 | 80 | 80 | 40 |
| MBC | 250 | 200 | 200 | 300 | 200 | 200 | 250 | 200 | 200 | 80 | 200 | 300 | 250 | 150 |
Conclusion
Show that by the animal experiment result of study Flos Lonicerae is secreted scorching clever sheet various dose and all the chronic prostatitis animal model had the obvious treatment effect.Simultaneously bacillary, two kinds of acute prostatitis models of non-bacterial also there are the obvious suppression effect, prove that this product has obvious therapeutic action to prostatitis.Test has also confirmed the antiphlogistic effects of this medicine, and the experimenter thinks that its anti-inflammatory activity may be the pharmacological basis of this product.This product has the obvious suppression effect to intraperitoneal mouse capillary permeability, the colloidality foot swelling of rat chondrus ocellatus Holmes and rat cott on pellet-induced granuloma formation.To infecting staphylococcus aureus and escherichia coli in the white mice body significant protective effect is arranged, external antibacterial, bactericidal assay result shows: to the strain of reference culture antibacterial colon bacillus, staphylococcus aureus strain, shigella flexneri strain; Bacterial strains such as clinical isolating staphylococcus aureus strain, staphylococcus epidermidis strain, staphylococcus saprophyticus strain all have certain antibacterial and bactericidal action.Show that this product has antiinflammatory, antibacterial, bactericidal action; In addition, this product also has effects such as tangible diuresis and minimizing white mice writhing response incidence rate.
In sum, Flos Lonicerae is secreted scorching clever sheet and is had and treat acute and chronic prostatitis (new indication) effect preferably, and this medicine also has many-sided pharmacological actions such as antiinflammatory, pain relieving, microcirculation improvement and diuresis simultaneously.
The specific embodiment
Embodiment 1: tablet
Prescription
Flos Lonicerae 270g, Herba Scutellariae Barbatae 136, Herba Dianthi 85g Herba Polygoni Avicularis 85g, Folium Pyrrosiae 85g, Caulis Akebiae 85g, Semen Plantaginis 85g, Herba Lophatheri 85g, Medulla Junci 28g, Herba Taxilli 136g makes 1000.
Method for making
80% of Flos Lonicerae, with 70% alcohol reflux 2 times, each 3 hours, add amount of alcohol and be followed successively by 8 times, 6 times of crude drug amount, merge extractive liquid,, filter, reclaim ethanol, standby when being concentrated into relative density and being 1.2～1.5 (60 ℃), other gets 9 flavors such as Herba Scutellariae Barbatae, Herba Dianthi and decocts with water and extract 3 times, and decocting time is followed successively by 1,5 hours, 1.5 hours, 1 hour, amount of water is followed successively by 10 times, 8 times, 6 times merge extractive liquid, of crude drug amount, filters, and is standby when being concentrated into relative density and being 1.10～1.35 (60 ℃).Half amount, decontamination, the coarse pulverization of extracting honeysuckle again merges with above two standby thick pastes, and drying is ground into fine powder, sieves, and makes granule, and drying is pressed into 1000, sugar coating, promptly.
Embodiment 2: capsule
Prescription
Flos Lonicerae 270g, Herba Scutellariae Barbatae 136g, Herba Dianthi 85g, Herba Polygoni Avicularis 85g, Folium Pyrrosiae 85g, Caulis Akebiae 85g, Semen Plantaginis 85g, Herba Lophatheri 85g, Medulla Junci 28g, Herba Taxilli 136g makes 1000 capsules.
Method for making
80% of Flos Lonicerae, with 70% alcohol reflux 2 times, each 3 hours, add amount of alcohol and be followed successively by 8 times, 6 times of crude drug amount, merge extractive liquid,, filter, reclaim ethanol, standby when being concentrated into relative density and being 1.2～1.5 (60 ℃), other gets 9 flavors such as Herba Scutellariae Barbatae, Herba Dianthi and decocts with water extraction 3 times, and decocting time was followed successively by 1.5 hours, 1.5 hours, 1 hour, and amount of water is followed successively by 10 times, 8 times, 6 times merge extractive liquid, of crude drug amount, filter, standby when being concentrated into relative density and being 1.10～1.35 (60 ℃).Half amount, decontamination, the coarse pulverization of extracting honeysuckle again merges with above two standby thick pastes, and drying is ground into fine powder, sieves, and makes granule, and drying is packed 1000 capsules into promptly.
Claims (3)
1. the application of pharmaceutical composition in the medicine of preparation resistance prostatitis, this pharmaceutical composition is made by following Chinese medicine raw materials by weight proportion, 125～375 parts of Flos Loniceraes, 62～187 parts of Herba Scutellariae Barbataes, 37～112 parts of Herba Dianthis, 37～112 parts of Herba Polygoni Avicularis, 37～112 parts of Folium Pyrrosiaes, 19～56 parts of Caulis Akebiaes, 37～112 parts of Semen Plantaginiss, 37～112 parts of Herba Lophatheris, 12～37 parts of Medulla Juncies, 62～187 parts of Herba Taxillis.
2. the application of pharmaceutical composition as claimed in claim 1 in the medicine of preparation resistance prostatitis, this pharmaceutical composition is made by following Chinese medicine raw materials by weight proportion: 187～312 parts of Flos Loniceraes, 93～156 parts of Herba Scutellariae Barbataes, 56～93 parts of Herba Dianthis, 56～93 parts of Herba Polygoni Avicularis, 56～93 parts of Folium Pyrrosiaes, 28～47 parts of Caulis Akebiaes, 56～93 parts of Semen Plantaginiss, 56～93 parts of Herba Lophatheris, 19～31 parts of Medulla Juncies, 93～156 parts of Herba Taxillis.
3. the application of pharmaceutical composition as claimed in claim 2 in the medicine of preparation resistance prostatitis, this pharmaceutical composition is made by following Chinese medicine raw materials by weight proportion: 190～270 parts of Flos Loniceraes, 110～136 parts of Herba Scutellariae Barbataes, 65～85 parts of Herba Dianthis, 65～85 parts of Herba Polygoni Avicularis, 65～85 parts of Folium Pyrrosiaes, 65～85 parts of Caulis Akebiaes, 65～85 parts of Semen Plantaginiss, 65～85 parts of Herba Lophatheris, 12～28 parts of Medulla Juncies, 110～136 parts of Herba Taxillis.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200710056354 CN101181540B (en) | 2007-11-27 | 2007-11-27 | Application of pharmaceutical composition in the preparation of medicine for prostatitis resistance |
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| CN 200710056354 CN101181540B (en) | 2007-11-27 | 2007-11-27 | Application of pharmaceutical composition in the preparation of medicine for prostatitis resistance |
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| CN101181540A true CN101181540A (en) | 2008-05-21 |
| CN101181540B CN101181540B (en) | 2013-01-23 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102552784A (en) * | 2012-02-05 | 2012-07-11 | 王永刚 | Preparation method of traditional Chinese medicine for treating prostatitis caused by fire excess from yin deficiency |
| CN101342216B (en) * | 2008-07-23 | 2012-07-18 | 孙庆敏 | Medicine for curing prostatitis and preparation method thereof |
| CN104042957A (en) * | 2014-06-25 | 2014-09-17 | 姜丽莎 | Traditional Chinese medicine (TCM) composition for treating prostatitis |
| CN112043808A (en) * | 2020-10-27 | 2020-12-08 | 禹州济德医院 | Traditional Chinese medicine preparation for treating prostatitis and preparation method thereof |
| CN114569680A (en) * | 2022-04-11 | 2022-06-03 | 吉林华康药业股份有限公司 | Composition for treating heat stranguria and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168488C (en) * | 2002-11-12 | 2004-09-29 | 吉林华康药业股份有限公司 | Chinese medicinal preparation for treating pyretic stranguria |
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2007
- 2007-11-27 CN CN 200710056354 patent/CN101181540B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101342216B (en) * | 2008-07-23 | 2012-07-18 | 孙庆敏 | Medicine for curing prostatitis and preparation method thereof |
| CN102552784A (en) * | 2012-02-05 | 2012-07-11 | 王永刚 | Preparation method of traditional Chinese medicine for treating prostatitis caused by fire excess from yin deficiency |
| CN102552784B (en) * | 2012-02-05 | 2013-06-19 | 王永刚 | Preparation method of traditional Chinese medicine for treating prostatitis caused by fire excess from yin deficiency |
| CN104042957A (en) * | 2014-06-25 | 2014-09-17 | 姜丽莎 | Traditional Chinese medicine (TCM) composition for treating prostatitis |
| CN112043808A (en) * | 2020-10-27 | 2020-12-08 | 禹州济德医院 | Traditional Chinese medicine preparation for treating prostatitis and preparation method thereof |
| CN114569680A (en) * | 2022-04-11 | 2022-06-03 | 吉林华康药业股份有限公司 | Composition for treating heat stranguria and preparation method and application thereof |
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| CN101181540B (en) | 2013-01-23 |
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