CN101072997A - Optical detector for a particle sorting system - Google Patents
Optical detector for a particle sorting system Download PDFInfo
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- CN101072997A CN101072997A CN 200480030084 CN200480030084A CN101072997A CN 101072997 A CN101072997 A CN 101072997A CN 200480030084 CN200480030084 CN 200480030084 CN 200480030084 A CN200480030084 A CN 200480030084A CN 101072997 A CN101072997 A CN 101072997A
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Abstract
To provide a display device having a shift register circuit capable of suppressing a current consumption from increasing. This display device is equipped with a shift register 30<SB>m</SB>. The shift register 30<SB>m</SB>includes a p-channel transistor PT3 which is connected to an HVSS side, a 1st circuit part 31<SB>m</SB>which is connected to a clock signal line, a p-channel transistor PT8 which is connected to the HVSS side, and a 2nd circuit part 32<SB>m</SB>which is connected to the clock signal line. The p-channel transistor PT3 turns on in response to a clock signal HCLK. The 1st circuit part 31<SB>m</SB>has a p-channel transistor PT5 which turns on in response to the output signal SR<SB>m+1</SB>(1st signal) of a shift regsiter 30<SB>m+1</SB>of a trailing stage to supply the clock signal HCLK to the p-channel transistor PT3. The p-channel transistor PT8 turns on in response to the clock signal HCLK. A 2nd circuit part 32<SB>m</SB>has a p-channel transistor PT10 which turns on in response to the output siganl SR<SB>m-1</SB>(2nd singal) of a shift register circuit 30<SB>m-1</SB>of a precedent stage to supply the clock signal HCLK to the p-channel transistor PT8.
Description
Related application
The present invention requires in the U.S. Provisional Patent Application sequence number 60/495 of submission on August 1st 4,2003,374 and the U.S. Patent Application Serial Number XX/XXX that submits on August 9th, 2004, the right of priority of XXX, the content of these patented claims is quoted as a reference by complete.
Technical field
The present invention relates to be used for the system and method that monitor flows is crossed the particle of passage.
Background technology
In system, can use optical system to monitor, analyze or detect this particle such as the microfluid system by one or more channel transfer particles.Optical system is useful in particle sorting system for example, and wherein this particle sorting system is classified to the particle flux that flows through one or more passages based on the predefine characteristic.
Traditional detection system has significant disadvantage.For example, because low luminance level (light level) signal of fluorescence labels on when light signal is expanded, being difficult to observe, make existing optical detection system out of true and bad result is provided sometimes on big zone from particle.Short when the light signal to be detected duration, during for example less than one millisecond, this light signal of existing optical system detection is also had any problem.For example, the frame frequency of traditional CCD (charge-coupled device (CCD)) technology is greater than one millisecond.
The existing system that is used for the inquiry microchannel also is subject to the single passage that focuses light rays at less than about 500 microns zone, and catches the light from similar limited area.
Summary of the invention
The invention provides the optical system that is used to gather from the quick spectrum of spatial channel array.This system is designed to be used for the analysis of inquiry microfluidic particle or the chip that contains at interval one or more horizontal fluid passage arrays of 1 to 200 millimeter is classified.The particle's velocity of transmitting in this passage is 0.1 to 10 meter of a per second, thus the duration of the signal that arrives of detector observes be inferior millisecond, need to adopt the detecting device and the electron device of 1 to 100 megahertz bandwidth to observe this signal.
This optical detection system comprises: be used to produce the light source of light beam, wherein this light beam passes micro-fluid chip to be monitored or passage; One or more lens or optical fiber are used for catching the particle of this light source and this microfluidic channel or the light that chemical substance interaction back forms; And one or more detecting devices.This detecting device can comprise the light amplification element, and detecting device detects each light signal and converts this light signal to electric signal.This electric signal of representing light signal strength respectively is delivered to the electronic data acquisition system from each detecting device and is used for analyzing.These one or more light amplification elements can comprise the image intensifier based on the hyperchannel flat board of photovoltaic array, multianode photoelectric tube or coupling photodiode detector array.
This optical system can be at once effectively and catch the delustring signal from one or more particle transmission channel arrays, one or more optical scattering signal and one or more fluorescence signal simultaneously, wherein these signals all have low intensity level and have high bandwidth (＞1MHz).This system provides and under various conditions the efficient and accurate of various particles has been monitored.
Description of drawings
To become apparent by this instructions and accompanying drawing the present invention, wherein the same reference numerals in the accompanying drawing is represented the same section in all different views.
Fig. 1 has set forth and has been applicable to and implements example embodiment of the present invention, has the synoptic diagram of the system of a plurality of passages that are used to transmit particle flux.
Fig. 2 is the synoptic diagram of optical detection of the present invention system.
Fig. 3 has set forth the cross section of running through a microchannel and being positioned at the plane vertical with this microchannel.
Fig. 4 is the synoptic diagram of optical detection of the present invention system, and it has elaborated the parts of this fluorescence detector.
Fig. 5 has set forth the optical detection system that is applicable to the particle in a plurality of passages of analyzing microfluid system.
Fig. 6 A to 6C shows in the optical detection system of Fig. 2, at the embodiment of the subsystem of an angle of 90 degrees or extinction position detection optical scattering.
Fig. 7 is the synoptic diagram of beam forming optical element that is applicable to the optical detection system of Fig. 2.
Fig. 8 has set forth the facet mirrors that is applicable to optical detection of the present invention system.
Fig. 9 is the partial view of the groove of Fig. 8 facet mirrors.
Figure 10 is the form that shows based on the difference configuration of the facet mirrors groove of corresponding spot width.
Figure 11 is the synoptic diagram that adopts the beam forming optical element of facet mirrors in the optical detection system of illustrated embodiments of the invention.
Figure 12 has set forth the suitable image intensifier that uses with the optical detection system of illustrated embodiments of the invention.
Embodiment
The invention provides the optical system that is used to monitor and detect the particle flux that flows through channel array.Below with reference to each example embodiment the present invention is described.Those skilled in the art will be appreciated that the present invention can be applicable to many different purposes and embodiment, and the present invention is not subject to the application in specific embodiment as described herein especially.
Fig. 1 has set forth and has been applicable to the microfluid system 10 of implementing example embodiment of the present invention, and this microfluid system comprises and being used for from transmitting a plurality of passages such as particle or cellular material therebetween.This illustrative microfluidic system 10 comprises substrate 1, and this substrate has a plurality of passages such as microchannel 3 that are placed in one.These passages are by microfluid system 10 transmitting fluids and/or particle, and wherein this microfluid system 10 is used to process, handle and/or carry out any suitable operation of fluid samples.Here employed term " microfluid " refers to be used to process, handle, get rid of and/or analyze the system or the device of the fluid sample that comprises at least one passage with microscopic dimensions.Here the path that permission such as the fluids that employed term " passage " refers to form in medium or run through medium moves.Term " microchannel " refers to preferably be formed at the passage in microfluid system or the device, and its sectional dimension scope is extremely about 500 μ m of about 1.0 μ m, is preferably about 25 μ m to about 350 μ m, most preferably is about 50 μ m to about 300 μ m.Those skilled in the art can determine the appropriate volume and the length of passage.These scopes are intended to comprise that above-mentioned value is as the upper limit or lower limit.This passage can have any selected shape or arrangement, and its example comprises linearity or nonlinear configurations and U-shaped configuration.Microfluid system 10 can comprise the microchannel 3 of any proper number, to be used for by microfluid system 10 transmitting fluids.
The invention provides the fluorescence detector that can use together with micro-fluid chip such as the microfluid system of Fig. 1.Thereby fluorescence detector of the present invention may be implemented in the measured zone 2 of microfluid system the described system of inquiry in this zone.The present invention helps to create scalable detection system to micro-fluid chip, wherein in micro-fluid chip, the array of parallel channels row portion of 1 to 200 passage is on one or more query area 2, and the physical width of this query area is 1 to 250mm, and preferable width is 1 to 100mm.
Fluorescence detector can monitor the fluid that flows through in a plurality of passages in the chip simultaneously.This fluorescence detector or optical sensor systems can be checked conspicuous other characteristic of one or more concrete properties such as size, shape, fluorescence intensity optical scattering and those skilled in the art of each particle.For example, in example embodiment, fluorescence detector of the present invention can place on the relatively large zone of chip (for example diameter is about 12 millimeters to 50 millimeters effective coverage), and this zone comprises the fluidized particle passage to be observed more than 100.This fluorescence detector can save local catch simultaneously from a plurality of or all passages fast, the signal of low luminance level.Those skilled in the art will appreciate that this optical system is not limited to be used for particle or cellular classification system, and can be used for having any appropriate system that flows through one or more passages to be monitored such as particulate material.
Fig. 2 has set forth the general view of the optical detection system 8 of illustrated embodiments of the invention, and this optical detection system can be used for the microfluid system of Fig. 1.Those skilled in the art will appreciate that this optical detection system can be used for any suitable system but not is limited to the microfluid system of Fig. 1.
Optical detection system 8 comprises light source 11, this light source is shown to laser and is coupled to beam forming optical element 12 to produce and to form the light beam 14 that passes optical mask 13, wherein this optical mask 13 be shown to micro-fluid chip 10 in the pinhole array of array alignment of particle transmission channel 3.The light of being permitted entering by pin hole passes transmission channel 3 itself subsequently.Permitted light beam that enters and the intersects particles of transmitting by passage 3 by one or more related pin holes by each passage, thereby produce light signal.In optical particle analysis, blood count or classification, the example of the light signal that can produce during with intersects particles when light beam comprises optical extinction, optical scattering and fluorescent light that angle is relevant.Optical extinction is meant the quantity of passing particle and not having interactional light.The optical scattering that angle is relevant is meant and is scattered or is bent and departs from the ratio of the light of incident beam with all angles (θ).Fluorescent light is meant by molecule absorption in the particle and the light launched once more long wavelength more.
Be positioned on the passage 3 detector optical element 15,16,17 on the side relative and catch and observe the light signal that intersects particles produced of light beam and passage with light source 11.Optical extinction detecting device 15 is set to light source 11 opposed, and aligns with the detection optical delustring with travel path of incident light 14.Optical scatter detectors 16 is set to and is substantially perpendicular to travel path of incident light 14, and wherein this path 14 is positioned at the plane that is formed by incident light vector and crossing microfluidic channel thereof.Preferably, this optical scatter detectors is positioned on the position with respect to 14 one-tenth about an angle of 90 degrees of travel path of incident light.Alternatively, can in same plane, place optical scatter detectors on these angles, to measure in other angle.Fluorescence detection subsystem 17 catches the light signal of autofluorescence.This fluorescence detection subsystem 17 can comprise large-sized high numerical aperture lens and subsidiary optical element.As shown in the figure, this fluorescence detection subsystem places micro-fluid chip 10 tops to catch fluorescent photon as much as possible and it is imaged onto on the detecting device (not shown).
Can realize optical detection system 8 in the query area 2 of chip 10.Shown in query area 2 comprise 24 passages 3, although those skilled in the art will appreciate that the passage that uses optical detection system 8 can observe any appropriate number.In the embodiment shown, this query area 2 is about 10mm wide (crossing over a plurality of passages 3) and multiply by 4mm long (along the direction of each passage 3), although one of ordinary skill in the art would recognize that this scope that the invention is not restricted to.
When the light 14 from laser 11 or other light source incides on the chip 10, the light that only passes the narrow district that particle flux crosses just can with particle interaction to produce light signal.The light that passes the light of the chip 10 that is positioned at outside the passage 3 or pass the passage area that does not comprise particle only has contribution and signal is not had contribution background or noise, so these light are parasitic light and should be minimized.Also to consider, pass this chip and do not pass and wait that the light of observing particle represented the laser source power that is wasted, therefore for the former of cost and radiating management thereby must minimize these light.Optical mask 13 that is formed by layer of pinholes and beam forming optical element 12 boths make minimum stray light and make the waste of laser power minimize.
As shown in the figure, light source 11 provides with respect to the incident light of passage 3 into about miter angle.According to this method, direct scattering/delustring is expanded at the opposite side upper edge of passage 3 equidirectional.As shown in the figure, 3 one-tenth miter angles of the propagation direction of direct scattering 14b and passage.The propagation direction of lateral scattering 14c becomes 90 degree with incident light, the mechanical degree of freedom 170 of taper is provided for fluorescence optics 17.Cone of mechanical degree of freedom 170 provides the unobstructed view (unobstructed view) of 90 degree for detecting device between direct scattering 14b and lateral scattering 14c.
Fig. 3 shows the schematic cross-section of a part that runs through the micro-fluid chip 10 that comprises a pair of microchannel 3a and 3b.This cross section is the pin hole 13a that passes microchannel and mask 13, the plane of 13b.Incident light 14 is stopped by layer of pinholes 13 parts, and incipient beam of light 14 narrows to form the focused beam acts 18 by each pin hole 13a, 13b definition.Focused beam acts 18 and each passage intersect the zone 31 that wherein allows particle 18 to flow through in routine nuclear (core flow) mode that flows with irradiation.Many parasitic lights are blocked by layer of pinholes 13, and this layer of pinholes can be separated with micro-fluid chip, and other method that perhaps can use photoetching or chip manufacturing those skilled in the art to know is produced on this layer of pinholes on the surface of chip.
This microfluid system comprises any system that flows through such as the passage of particle or cellular material that is useful on that contains.For example, microfluid system 10 can comprise particle sorting system, and as the particle sorting system described in U.S. Patent Application Serial Number 10/179488 and 10/329008, the content of these two patented claims is referred to herein as a reference.Described other microfluid system that is suitable in U.S. Patent Application Serial Number 10/028852,10/027484,10/027516 and 10/607287, all these patented claims are incorporated herein by reference.
Fig. 4 has set forth the synoptic diagram of the optical detection system of Fig. 2, and this figure has at length set forth the parts of fluorescence detection subsystem 17.Fluorescence detection subsystem 17 comprises the convergent lens 45 of high-NA (low F#), and these lens are configured and are placed to the seizure photon from illuminated particle emission as much as possible.Lens 45 can be the F#=1 lens of the 50mm that can be purchased off the shelf, and its focal length is obtainable on the market.One is exemplified as Leica Noctilux 50mm F# 1 lens.Can also obtain bigger lens and use it for the imaging multi-layer planar.The dispersion element 46 that is shown to the Littrow grating is positioned on first convergent lens 45.Dispersion element 46 is according to the wavelength of concrete light beam and crooked this light.Shown in the diameter of Littrow grating 46 be 76.2mm, useful area is 73mm.Littrow grating 46 every mm have 720 grooves, are 43.1 degree (angle of grating offset from perpendicular) at the blazing angle of 550nm.The Littrow angle is 23.33 degree, and promptly the light of 550nm is bent the angle of offset from perpendicular among Fig. 4.Those skilled in the art will appreciate that according to instruction of the present invention to use any proper tools with special mode bend light rays.Place reconstruction lens 47 with the single order diffracted ray of seizure with the Littrow angle, and diffracted ray is reconstituted the image of illuminated particle in as plane 48 from grating 46.
Owing to there is the Littrow grating in light path, when the particle in the irradiated microchannel 3 was imaged onto in the plane 48, the angle that long wavelength photons is tilted was greater than the angle that short wavelength photons is tilted, and makes this particle have spectrum widening on as the plane.When lens 45 and 47 used the lens of 50mm focal lengths, wavelength is 500nm to the photon of 700nm had been about 7841 microns as the broadening in the plane 48.The spectral resolution of illustrated embodiment is 39.2 microns every nano wave lengths.
Optical detection system 8 can be used for observing the particle that is marked with antibody, and wherein this antibodies is to the known fluorophore of blood count those skilled in the art or other fluorescent grain mark.When excitation wavelength is 488nm, the particle that antibody for example can usage flag be arranged, this antibodies is to fluorophore FITC (fluorescein isothiocynate), PE (phycoerythrin), APC (allophycocyanin) and PerCP (perdinin phyllochlorin), and the emission of the peak fluorescence of these fluorophores lays respectively at 530nm, 575nm, 630nm and 695nm.To place the position (is 0 as reference with 550nm) of-784 microns, 980 microns, 3136 nanometers on the picture plane and 5684 microns respectively from the photon of FITC, PE, APC and PerCP.Opaque flat board has 400 microns hole and placed diameter in these holes is 400 micron fiber, and this opaque flat board will make each optical fiber 49 have the wavelength pullin banwidth that is about 10nm.Optical fiber 49 is placed and the corresponding position of the peak emission of desirable fluorophore, can form effective and compact polychrome detection system.One end of optical fiber 49 is positioned at picture plane 48, and the other end is connected to detecting device.In the embodiment shown, the second end of optical fiber is being coupled to the photocathode window (for example H6780-20 of single anode of Hamamatsu company production or the H7260-20 photoelectric tube of 32 anodes) of photoelectric tube with the corresponding position of single anode, thereby amplifies the fluorescence light signal and convert thereof into electric signal.Also can use other light amplification detecting device of knowing such as image intensifier or avalanche photodide array or optical field technician to come sensed light signal and convert thereof into electric signal.
In Fig. 4, the optical fiber 49 of the particle shown in the inquiry in the passage is positioned at and identical plane, micro-fluid chip internal channel plane.If on multichannel array, use this system, before the plane of passage shown in then other passage is positioned at or after the plane of passage shown in being positioned at.
Fig. 5 shows the skeleton view of the optical sensor systems 80 that is used for observing a plurality of passages of micro-fluid chip.Optical sensor systems 80 also comprises pinhole array 13, and the incident light 14 that the blocking-up of this pinhole array is most also shines little surveyed area 2 in each passages 3 of six passages of micro-fluid chip.The optical column of convergent lens, Littrow grating and reconstruction lens is similar to shown in Figure 4, and can have identical lens and grating specification embodiment.Usually, the component size of lens and grating group must be enough to form the visual field greater than the size of surveyed area (by the zone of the irradiated passage of pin hole) on chip.Placed the flat board 480 that supports six arrays 490 in as plane 48, each array comprises four optical fiber respectively.Each array of placing four optical fiber 49 is with the spectrum of sampling from association channel 3 emissions.Each optical fiber of this array places the peak emission location of a fluorophore.Here be fit to use the optical fiber or the lensed optical fiber of high-NA, those skilled in the art will be appreciated that this point.
Fig. 6 A to 6C shows the embodiment of the subsystem of the optical scattering that is used to detect an angle of 90 degrees or delustring.In the present embodiment, optical extinction column (columnated) detecting device band 63 places on the multi-channel chip 10 that is about 500 microns between the passage at interval.Fig. 6 B shows the cross section of optical extinction column detecting device band 63, this detecting device band is such mechanical component, the diameter of boring is 300 microns, and drilling depth is less than tape thickness 63d, thereby and between this mechanical component center spacing be 500 microns and alignd with channel pitch in these holes.Place high numerical aperture fiber 65 to form fiber array 61 in each hole, wherein each passage is equipped with an optical fiber.But in each hole, get out diameter littler with optic fibre hole 63c be concentrically ringed cylinder hole.This cylinder hole passes Belt connector 63b, allows light to pass cylinder hole 63c and arrive to place in the interior optical fiber 65 of the bigger axle of diameter.In order to make this subsystem work, incident light 68 is to intersect near miter angle and pin hole and passage, and optical extinction detecting device band 63 is directly installed (promptly becoming 180 degree angles with incident light) along the incident light vector, as schemes shown in the position of this band.The aperture of post device (Columnator) must surpass the aperture of pin hole, makes that for by the incident light of strict stylolitization, all light that pass pin hole can be detected in the optical fiber of post device end.Thereby select sufficiently long post device to remove any parasitic light from other passage.For example, in one embodiment, pinhole aperture is 150 microns, and post device diameter is 250 microns, and fibre diameter is 300 microns, and placing the length of the post device in the passage of 2mm is 1mm.At the far-end of fiber array 61, each optical fiber is attached to photoelectric tube or other fluorescence detector.Optical extinction is normally bright enough, can use photodiode as its detecting device.
In Fig. 6 C, second is with 63 substantially the samely with 66 structure and described first, but second be with 66 to be set to incident light and to become an angle of 90 degrees, and this angle is applicable to 90 degree scattering or the sidescattering signals of measurement from cell or particle.Those skilled in the art will appreciate that to place similarly in other angle and be with to observe other scattering parameter.Interested special angle be along almost forwards to the so-called direct scattering of optical scattering, this is forwards to need not to gather straight-through light near directly placing (almost becoming 180 degree with incident direction) forward on the delustring path usually.
In yet another embodiment, light source 11 is Coherent Sapphire 488/200 laser instrument, and this is little, the air cooled solid-state device of a kind of volume, produces the laser of about 200mW from Gas LASER Tube emission, and the noise of this laser instrument is very little or do not have a noise.Alternatively, can use OPSS (optically pumped solid-state) laser instrument, this laser instrument also can produce all required different excitation wavelengths of execution monitoring.Those skilled in the art will appreciate that and to use any appropriate light source.
The sectional view of an embodiment of the beam forming optical element 12 that Fig. 7 uses for the fluorescence detector that is fit to illustrated embodiment of the present invention.Draw this optical schematic diagram in the x-z plane, wherein the general direction of light transmission is along the z axle.Each dotted line is directed upwards towards the x-y section sketch 14 ' of light beam, is how to handle this light beam so that the moulding optical element to be shown.The cross section of this light beam when single laser instrument 11 is exported is the sub-circular of 700 microns of diameters, becomes the wave length filtering bundle after through low pass or bandpass filter 74.This light beam is subsequently through first pair of cylindrical collimation 73, and the focal length of these lens is 5mm, and focal length is 250mm, and these lens produce the light beam that is essentially rectangle.This light beam passes cylindrical lens subsequently and focal length is the condenser lens 71 of 150mm, thereby makes light beam 14 sharpen into 100 microns on the y axle.Whole sectional view in the present embodiment after the process condenser lens 71 is that 36mm multiply by 100 microns, and can be used for shining the pinhole array 13 that is spaced apart 500 microns and reaches 70 pin hole/passages.Because these pin holes less than about 100 microns, have prevented the waste of light at the y direction of principal axis to the restriction of light beam.On N pinhole chip of 500 microns of center distance, preferably make this light beam be slightly larger than 500 * N micron along the x axle, be 200 microns (being slightly larger than 100 microns) along the y axle, thereby make the laser power of being wasted minimize.This column and shaped beam intersect with pinhole array 13 subsequently, and become and be separated N the pin hole shaped beam 78 that intersects with the coupling array with passage 3.
The beam forming embodiment of Fig. 7 is very useful, can realize minimum parasitic light and be about 10% acceptable power efficiency, in view of quick (bandwidth is greater than 10MHZ) delustring, scattering and fluorescence from many passages can be once observed in this design simultaneously.
Fig. 8 shows the reflective beam splitter 80 based on grooved mirror that is applicable to optical detection of the present invention system.Beam splitter 80 comprises the facet mirrors 83 that is used for incident beam is split into a plurality of light beams.Column incoming beam 82 enters beam splitter 80, and on incident mirror 81, be reflected, this incident is used to be provided with the correct incident angle (be generally low-angle) of light beam on facet mirrors 83 through 81, and this facet mirrors splits into the more array 84 of penlight with incident beam.This more the array 84 of penlight extend upward, and be parallel to incoming beam 82.
Fig. 9 shows design point of view and the formula that instructs this facet mirrors.Incident beam 82 is reflected, and each groove 83a partly prunes in the mirror, and the part of being pruned with the fixed angle reflection and form narrower light beam 84a.Form the second arrow beam of light 84b by adjacent grooves 84b.Be spaced apart groove pitch A between each groove, it is the light beam of L that beam splitter produces even spot width (supposing that groove is uniform) and light beam or channel pitch, wherein this spacing L is designed to micro-fluid chip in pin hole and channel spacing be complementary.
Figure 10 is the form of the embodiment of Fig. 8 beam splitter, and wherein channel spacing L is 500 microns and uses silicon anisotropic etching to make these grooves (fixing groove angle e=54.74).This form has been pointed out the appropriate mirror arrangement of selected spot size.For example, 100 microns spot sizes are suitable for the pin hole less than 100 microns, corresponding to the groove pitch of A=575 micron, the ditch groove tilt angle of G=29.7 degree and the incident angle of I=25 degree.
Figure 11 shows the embodiment of the beam forming subsystem 112 that is suitable for optical sensor systems.This schematic beam forming subsystem 112 adopted to the similar beam forming optical element 12 of described beam forming optical element 12 with reference to figure 7 after final stage utilized facet mirrors 80 such as Fig. 8 facet mirrors.
Alternative is included in makes pinhole array 13 on each micro-fluid chip, rather than this array is installed on this optical system discretely.
Fig. 4 and Fig. 5 as the plane in the alternative of detecting device of employed fiber array then be image intensifier is placed in this plane and optical fiber placed image intensifier after to read out in the light signal that produces on its fluorescent powder.By all fluorescence signals only being used a light amplification element (image intensifier), and use photodiode to convert the light signal after the image intensifier to electric signal, this alternative can reducing cost.
Figure 12 shows the image of standard Hamamatsu image intensifier 220, but those skilled in the art will appreciate that at this and can use any large tracts of land light amplification element with high spatial resolution in alternative.Image intensifier 220 is used for amplifying the intensity of optical imagery before passing the signal to photodiode array or any other suitable pick-up unit.As shown in the figure, this image intensifier comprise picture signal input window 221, be used for light be transformed into photoelectronic photosensitive electronic emitter such as photocathode 222, be used for electron multiplication MCP 223, be used for the video screen 224 of electronic switch Cheng Guang and the output window 225 that is shown to fibre optic faceplate (fiber optic plate).According to an illustrated embodiment, image intensifier can comprise the Hamamatsu image intensifier of 25mm-40mm, can use any suitable device although one of ordinary skill in the art would recognize that.
Both alternatives of beam forming subsystem 12 and fluorescence detection subsystem 17 comprise short logical or long logical or wavelength band is logical or rejection filter, thereby in fluorescence detecting system, eliminate spuious or (spurious) source light that looks genuine, perhaps from the light of light source 11 emissions, remove spuious or the wavelength component of looking genuine.
An alternative of delustring and scatter detector 15,16 is, adds a laser power monitor independently in system, is used for these signals of normalization.This point is useful, and this is because those signals all are directly proportional with laser power, so the noise on the laser can make these distorted signals.
With detecting device 15,16, and an alternative of 17 fiber arrays that use together be to use photodiode or avalanche photodide array or other fluorescence detector array to substitute each fiber array.Those skilled in the art will appreciate that here and can adopt alternative detecting device, as long as the intensity level of these detecting devices coupling samples requires and the form factor requirement of the concrete chip embodiment that will use.
The alternative of beam splitter can be used reflective groove arrays manufactured, wherein can be by anisotropically corroding crystalline material or also carrying out suitable optical polish subsequently or apply reflectance coating making this reflective groove arrays manufactured by tradition processing or formation plastics to metal.
In all embodiment of the present invention, pinhole array mates with microfluidic channel on spacing usually.When using reflective beam splitter in the beam forming optical element, this beam splitter also must mate with pin hole.
Although the simplest implementation method has been used the passage of arrayed equably and the pin hole of arrayed and the groove that divides intrafascicular arrayed equably equably, but the present invention does not have these requirements, and similar embodiment can be designed so that with irregular channel spacing or figure.
The alternative of fluorescence detection subsystem A7 is, on picture plane (3-5), adds narrow-band pass filter before or after (2-8).400 micron fiber will be caught the bandwidth of 10nm in this plane.Adding the bandpass filter of 10nm or 5nm will improve sensitivity in some cases and reduce noise.
Reference example embodiment has described the present invention.Owing to do not leave scope of the present invention and can not carry out specific change to above-mentioned structure, thus all things shown in foregoing description or the accompanying drawing all should be understood as that be set forth character and non-limiting matter.
Should also be clear that following claims will cover all general featuress of the present invention as described herein and concrete feature, all statements of the scope of the invention are all dropped within the scope of following claims.
Claims (12)
1. optical detection system that is used to observe microfluid system, this microfluid system comprises the channel array that is used to transmit particle or molecule, and this optical detection system comprises:
Be used to produce the light source of light beam;
Be used to focus on a beam combination moulding optical element of this light beam;
Pinhole array, each pin hole is complementary with related with the interior microfluidic channel of the interior described channel array of microfluid system;
At least one row shape detecting device band is used to detect this light beam and passes one of optical extinction, forward scattering and side-scattered of the back formation of one of described passage by one of described pin hole; And
The fluorescence detector of high-NA is used for receiving the light signal that this particle produces when the particle of arbitrary described channel array and described light beam interact.
2. one kind is used reflective beam splitter that single incident beam is formed the system of the controlled more penlight array of spacing, and this system comprises:
The uniform array of reflection groove; And
Be used for reflective groove arrays manufactured being presented to the steady arm of incoming beam with selected angle.
3. optical detection system that is used for the microfluid system that comprises the microfluidic channel array that transmits particle is carried out inquiry, this optical detection system comprises:
Be used to produce the light source of light beam;
Comprise a beam combination moulding optical element of reflective beam splitter, be used for this beam splitting is become a plurality of auxiliary beams;
With the pinhole array that microfluidic channel array in this microfluid system is complementary, wherein this beam forming optical element passes one of described pin hole with each described a plurality of auxiliary beams guiding.
4. optical detection system that is used to observe microfluid system, wherein this microfluid system contains the passage of transmission particle or molecule, and this optical detection system comprises:
Be used to produce the light source of light beam;
Be used to focus on a beam combination moulding optical element of this light beam;
With the pinhole array that this microfluidic channel is complementary, wherein this beam combination moulding optical element makes this light beam pass described pinhole array;
At least one row shape detecting device band; And
The fluorescence detector of high-NA is used for a plurality of passages in this microfluid system of inquiry simultaneously and detects three fluorescence wave bands simultaneously.
5. optical detection system that is used to observe microfluid system, wherein this microfluid system comprises the passage of transmission particle or molecule, this optical detection system comprises:
Be used to produce the light source of light beam;
Be used to focus on a beam combination moulding optical element of this light beam;
The pin hole that is connected with the passage of this microfluid system; And
The fluorescence detector of high-NA is used for a plurality of passages in this microfluid system of inquiry simultaneously and uses image intensifier as the optics amplifier element.
6. optical detection system that is used to observe microfluid system, wherein this microfluid system comprises the passage that is used to transmit particle or molecule, this optical detection system comprises:
One or more laser, this laser shines a spatial spread array of spots by a mask at the speckle displacement opening;
First high numerical aperture lens, these lens are set to the light of catching at once from whole spatial spread array of spots;
The spectral separation element is used to receive and crooked light from first lens;
Second high numerical aperture lens, be used to catch by the light of this spectral separation element bending and with the image formation by rays of being caught to second pinhole array;
Be positioned at second pinhole array image intensifier afterwards, be used to detect and amplify the light that passes this pinhole array; And
Photodiode detector array is used to catch and change the light from image intensifier.
7. the detecting device of claim 6 further comprises and is coupled to this photodiode detector to gather the electronic data acquisition system from the electronics output of this photodiode detector.
8. the detecting device of claim 6 further comprises the laser wavelength blocking filter that places between described array of spots and the image intensifier.
9. the detecting device of claim 6, wherein this detecting device can amplify from a plurality of spots of spatial spread and the duration less than one millisecond low-intensity spectrum, and can convert this spectrum to electric signal.
10. optical system comprises:
Light source is used to produce and passes the light beam for the treatment of monitored object;
Lens are used to catch the light from this light source; And
The detecting device that comprises the light amplification element is used for sensed light signal and converts described light signal to electric signal.
11. the optical system of claim 10, wherein this light amplification element comprises photovoltaic array.
12. the optical system of claim 10, wherein this light amplification element comprises the image intensifier based on the hyperchannel flat board that is coupled to photodiode detector array.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US49537403P | 2003-08-14 | 2003-08-14 | |
| US60/495.374 | 2003-08-14 | ||
| US10/915,016 | 2004-08-09 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910173676A Division CN101776599A (en) | 2003-08-14 | 2004-08-16 | Optical detector for a particle sorting system |
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| Publication Number | Publication Date |
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| CN101072997A true CN101072997A (en) | 2007-11-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200480030084 Pending CN101072997A (en) | 2003-08-14 | 2004-08-16 | Optical detector for a particle sorting system |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN101072997A (en) |
| ZA (2) | ZA200601273B (en) |
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| CN102272580A (en) * | 2010-03-31 | 2011-12-07 | 古河电气工业株式会社 | Optical information analysis device and optical information analysis method |
| CN104487818A (en) * | 2012-06-19 | 2015-04-01 | 美利坚合众国政府,由海军部长所代表 | Remote multisensor optical particle monitor for flowing fluid systems |
| CN104990907A (en) * | 2015-07-01 | 2015-10-21 | 上海睿钰生物科技有限公司 | Automatic multichannel flow-like image fluorescence analysis system |
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2004
- 2004-08-16 ZA ZA200601273A patent/ZA200601273B/en unknown
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- 2004-08-16 ZA ZA200704831A patent/ZA200704831B/en unknown
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| Publication number | Publication date |
|---|---|
| ZA200601273B (en) | 2007-09-26 |
| ZA200704831B (en) | 2009-08-26 |
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