CN101029895A - Method for inspecting blood conservative-liquid intracellular toxin of blood-collecting bag - Google Patents
Method for inspecting blood conservative-liquid intracellular toxin of blood-collecting bag Download PDFInfo
- Publication number
- CN101029895A CN101029895A CN 200610059048 CN200610059048A CN101029895A CN 101029895 A CN101029895 A CN 101029895A CN 200610059048 CN200610059048 CN 200610059048 CN 200610059048 A CN200610059048 A CN 200610059048A CN 101029895 A CN101029895 A CN 101029895A
- Authority
- CN
- China
- Prior art keywords
- blood
- anticoagulant
- storage
- conservative
- collecting bag
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A method for detecting bacterial toxins of blood storage liquid in blood sampling bag includes taking Ca(OH)2 and drying it under high temperature to remove off heat source, preparing it to be saturated solution, adding blood storage liquid to dilute said saturated solution to be diluted solution of certain times and carrying out detection by utilizing king crab reagent according to gel method listed in bacterial toxins detection manner of XIE appendit on 2005 version of Chinese pharmacopeia.
Description
Technical field
The present invention relates to a kind of detection blood conservative-liquid intracellular toxin of blood-collecting bag method.
Background technology
The anticoagulant for storage of whole blood bacterial endotoxin can cause patient's pyrogen reaction in the blood taking bag, present national current law and standard " have stipulated that the limit value of the bacterial endotoxin of anticoagulant for storage of whole blood is<5.56EU/ml in Chinese pharmacopoeia and " national drug standards ", and two kinds of detection methods of pyrogen or bacterial endotoxin, i.e. rabbit method and tachypleus amebocyte lysate detection of bacterial endotoxin (BET) method have been stipulated to detect.As detecting with the rabbit method, must be equipped with Animal House and the animal that meets experimental standard, and the feeding and management personnel of animal used as test and a series of auxiliary facilities etc., expense is higher, and this is fit to for the blood taking bag production unit, and for Blood Center or blood station, only being to the layer sampling observation, is not too to be fit to.Use BET detection method compatible blood center or blood station, but because of citric acid radical ion in the anticoagulant for storage of whole blood can have interference effect to limulus test, therefore the thin ups that adopt disturb to get rid of more, operation steps is many, error is big but it exists, can cause anticoagulant for storage of whole blood increased and the test result instability problem by the environmental pollution probability, increase owing to adding water simultaneously, require the sensitivity of the limulus reagent height, increased cost.
Summary of the invention
Purpose of the present invention provides a kind of detection blood conservative-liquid intracellular toxin of blood-collecting bag method for people exactly, calcium hydroxide [Ca (OH)
2] become saturated solution, mix with anticoagulant for storage of whole blood and detect through doing baking, can get rid of the interference of citric acid radical ion in the anticoagulant for storage of whole blood fast, effectively.
The objective of the invention is to realize: with the stable calcium hydroxide of high temperature resistant physics and chemistry [Ca (OH) by following technical method
2], high temperature is prepared saturated solution after doing roasting depyrogenation, gets the certain multiple dilution with the anticoagulant for storage of whole blood mixed diluting, detects with tachypleus amebocyte lysate then.
Described concrete steps:
1., take a morsel and analyze pure cerium hydroxide calcium [Ca (OH)
2] put in the clean glass container, in baking box, do roasting depyrogenation more than 1 hour for 250 ℃, add the apirogen water dissolving and make saturated solution.
2., the supernatant of getting above-mentioned solution is earlier with 11 times of anticoagulant for storage of whole blood dilutions, again with 2 times of apirogen water dilutions, 22 times of dilutions of anticoagulant for storage of whole blood.
3., with the tachypleus amebocyte lysate of sensitivity 0.25EU/ml, according to " method 1 (gel method) detects in Chinese pharmacopoeia version appendix in 2005 the XI E bacterial endotoxins test.
Advantage of the present invention is compared with existing rabbit method detection, and facility and equipment are simple, expense is low; Detect the dilute with water anticoagulant for storage of whole blood with the BTE method and compare, operation steps is few, error is little, is subjected to the environmental pollution probability few, test result is stable, the requirement sensitivity of the limulus reagent is low, cost is low, can fast get rid of the interference of citric acid radical ion in the process of the test, is specially adapted to Blood Center or blood station and uses.
Embodiment
Embodiments of the invention are to utilize calcium hydroxide [Ca (OH)
2] the stable character of high temperature resistant physics and chemistry, take a morsel and analyze pure Ca (OH)
2Put in the clean glass container, in baking box, do roasting depyrogenation more than 1 hour for 250 ℃, add the apirogen water dissolving again and make saturated solution, the supernatant of getting this solution is earlier with 11 times of anticoagulant for storage of whole blood dilutions, again with 2 times of apirogen water dilutions, 22 times of dilutions of anticoagulant for storage of whole blood, and then with the tachypleus amebocyte lysate of sensitivity 0.25EU/ml, according to " method 1 (gel method) detects in Chinese pharmacopoeia version appendix in 2005 the XI E bacterial endotoxins test.
Claims (2)
1, a kind of detection blood conservative-liquid intracellular toxin of blood-collecting bag method is characterized in that with the stable calcium hydroxide of high temperature resistant physics and chemistry [Ca (OH)
2], high temperature is prepared saturated solution after doing roasting depyrogenation, gets the certain multiple dilution with the anticoagulant for storage of whole blood mixed diluting, detects with tachypleus amebocyte lysate then.
2, detection blood conservative-liquid intracellular toxin of blood-collecting bag method according to claim 1 is characterized in that concrete steps:
1., take a morsel and analyze pure cerium hydroxide calcium [Ca (OH)
2] put in the clean glass container, in baking box, do roasting depyrogenation more than 1 hour for 250 ℃, add the apirogen water dissolving and make saturated solution,
2., the supernatant of getting above-mentioned solution is earlier with 11 times of anticoagulant for storage of whole blood dilutions, again with 2 times of apirogen water dilutions, 22 times of dilutions of anticoagulant for storage of whole blood,
3., with the tachypleus amebocyte lysate of sensitivity 0.25EU/ml, according to " method 1 (gel method) detects in Chinese pharmacopoeia version appendix in 2005 the XI E bacterial endotoxins test.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200610059048 CN101029895A (en) | 2006-02-28 | 2006-02-28 | Method for inspecting blood conservative-liquid intracellular toxin of blood-collecting bag |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200610059048 CN101029895A (en) | 2006-02-28 | 2006-02-28 | Method for inspecting blood conservative-liquid intracellular toxin of blood-collecting bag |
Publications (1)
Publication Number | Publication Date |
---|---|
CN101029895A true CN101029895A (en) | 2007-09-05 |
Family
ID=38715358
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 200610059048 Pending CN101029895A (en) | 2006-02-28 | 2006-02-28 | Method for inspecting blood conservative-liquid intracellular toxin of blood-collecting bag |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101029895A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104903725A (en) * | 2012-12-12 | 2015-09-09 | 艾维萨制药公司 | Determination of location of bacterial load in the lungs |
CN111141909A (en) * | 2019-12-24 | 2020-05-12 | 南京健友生化制药股份有限公司 | Method for detecting bacterial endotoxin in thioglycerol by gel method |
-
2006
- 2006-02-28 CN CN 200610059048 patent/CN101029895A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104903725A (en) * | 2012-12-12 | 2015-09-09 | 艾维萨制药公司 | Determination of location of bacterial load in the lungs |
CN111141909A (en) * | 2019-12-24 | 2020-05-12 | 南京健友生化制药股份有限公司 | Method for detecting bacterial endotoxin in thioglycerol by gel method |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Lu et al. | Nanomaterials as assisted matrix of laser desorption/ionization time-of-flight mass spectrometry for the analysis of small molecules | |
Yang et al. | Gold‐Doped Covalent Organic Framework Reveals Specific Serum Metabolic Fingerprints as Point of Crohn's Disease Diagnosis | |
CN203519585U (en) | Air quality detection device | |
CN101029895A (en) | Method for inspecting blood conservative-liquid intracellular toxin of blood-collecting bag | |
CN103969411B (en) | A kind of method directly measuring soluble organic nitrogen concentration in water body based on electrodialysis pretreatment mode | |
Xiang et al. | Analysis of children's serum fluoride levels in relation to intelligence scores in a high and low fluoride water village in China | |
CN205851046U (en) | A kind of medical treatment internal medicine blood sugar test paper quick crashing device | |
CN102323105A (en) | Method for sampling electrolyte of lithium ion battery in closed mode | |
CN102228808B (en) | Composite mesoporous film of PETP (polyethylene glycol terephthalate) as well as preparation method and application thereof | |
CN104089997B (en) | A kind of electrochemical immunosensor and its preparation method and application | |
CN101055270A (en) | Semen energy quick detection reagent kit and its preparation and usage method | |
CN205981818U (en) | A MALDI target plate that is used for sample to fix in detection of chip carrier normal position | |
CN102692494B (en) | Endotoxin detection method for nano-particle size analyzer | |
CN204500785U (en) | A kind of secretions specimen sampling pen | |
CN104698159A (en) | Method for detecting endotoxin content | |
CN104345151B (en) | Improve the specific membrane enclosure method of elispot assay | |
CN203965305U (en) | A kind of Ozone Monitor | |
CN103289929A (en) | Novel quick separation method of bacilluscereus | |
CN102716072A (en) | Method for preparing ciprofloxacin lactate sodium chloride injection | |
CN102841205A (en) | Method for detecting fleroxacin bacterial endotoxin used for injection | |
Saardpun et al. | The Finding of New In Vivo Metabolite Triptorelin (5-10) in Human Urine Using Liquid Chromatography Coupled with Ion Trap/Time-of-Flight Mass Spectrometry with Dimethyl Sulfoxide Additives in the Mobile Phase | |
Ning et al. | Protective Effects of Different Molecular Weights of Purslane (Portulaca oleracea L.) Aqueous Extract on DSS-Induced Ulcerative Colitis in Mice | |
CN109580827A (en) | A kind of improved method of livestock meat veterinary drug residue measurement pretreatment process | |
CN115792044B (en) | Method for identifying whether enoxaparin sodium from pig source is doped with enoxaparin sodium from sheep by utilizing enoxaparin sodium reduction end | |
CN102435640A (en) | Hotwash oil inspection method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20070905 |