CN100427194C - Microfluid dynamic mixer, mixing pond mould in chip and driving method thereof - Google Patents
Microfluid dynamic mixer, mixing pond mould in chip and driving method thereof Download PDFInfo
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- CN100427194C CN100427194C CNB2004100110633A CN200410011063A CN100427194C CN 100427194 C CN100427194 C CN 100427194C CN B2004100110633 A CNB2004100110633 A CN B2004100110633A CN 200410011063 A CN200410011063 A CN 200410011063A CN 100427194 C CN100427194 C CN 100427194C
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Abstract
The present invention relates to a microfluid dynamic mixer in a chip, which comprises a mixing pond 1, a collimator 2, an optical fiber 3, a positioning substrate 4, a bracket 5, a vibrating film 6, an alternating current power supply 7, a plane coil 8, a permanent magnet slice 9, an optical fiber 10 and a collimator 11. A driving method comprises the steps that the alternating current power supply is used for switching on current in a square wave form in the plane coil to make the plane coil generate an alternating magnetic field; the permanent magnet slice is used for forming a magnetic field which interacts with the alternating magnetic field for driving the vibrating film to move back and forth to make the volume of the mixing pond change for driving the microfluid dynamic mixer in a chip. A mould comprises a groove 20, an open mould body 21, a square column 22, a groove 23, a cylinder 24, a square column 25, a convex mould body 26, a cylinder 27, a mould opening threaded hole 28, a mould opening screw 29, a mould opening threaded hole 30, a mould opening screw 31, a concave mould body 32, a groove 33, a positioning plane 34, a square column 35, a groove 36, a bottom groove 37 and a square column 38.
Description
Technical field:
The invention belongs to micro-electromechanical system field, relate to micro liquid dynamic mixer in the chip.
Background technology:
Microfluid system can be applicable to fields such as chemical analysis, biology and chemical sensitisation, molecular separation, nucleic acid ordering and analysis, environmental monitoring.Fully mixing is the necessary condition of carrying out biochemical reaction, but tradition obviously is not suitable for biochemical chip by the power mixed method that mixes.Therefore inquired into by many scholars and can realize the microlitre or the new method that rises the mixing of magnitude liquid of receiving in the chip.
Existing in the world at present dissimilar micro-mixers are broadly divided into dynamic mixer and static mixer.
In dynamic mixer, it is mainly by microfluidic channel, microreactor with add the field of force and wait the married operation of realizing sample.1991, Moroney and White used the ultrasonic pump drive fluid to form fluid circuit, have realized fabulous mixing.Evan flowed in the mixed cabin (area is 1.6 * 0.6mm, and 100 μ m are dark) of steam bubble in Multiple Cycle with the high temperature generation back and forth in 1997.Make that adjacent fluid particles is separated in a large number in chaos flow field (movement locus of its initial conditions convection cell molecule cuts much ice with the final position), polymerization, fluid molecule forms the chaos convection current, and fluid is mixed fully.Miyake utilized the feature of fluid by miniature spout in 1993, designed a kind of micro-mixer that can produce many rills.It as fluid line, has the Mixed Zone of 2.2mm * 2mm * 330 μ m with microchannel in the porch of reagent, 400 miniature spouts that are spaced apart 15 μ m are arranged at the bottom.By these spouts, top sample is injected in the following reagent, has formed a lot of small jet flows, i.e. the jet flow array.Jet flow can increase the contact-making surface on two kinds of fluid-mixing surfaces greatly, by spreading the speed of quickening to spread.
In static mixer, it mainly reaches effective mixing by elongating or shear laminar flow (increasing the contact area between liquid) or shunting (big liquid flow split is become many little liquid streams, the thickness of liquid is reduced greatly).The realization of above target mainly reaches by geometry that changes micro-tube in the blender or the relative motion that produces between liquid stream.Using more method at present is according to the comparatively complicated continuous or parallel flow path system of microfluid mechanics principle design, to strengthen the convection current and the molecular diffusion of microfluid, increase effective contact area of microfluid, improve the mixing efficiency of liquid, reduce and mix required time and space.Alcatel Optronics Netherlands company has developed a kind of layer-stepping mixer structure, and liquid repeatedly converges, thereby reaches the good mixing effect through repeatedly shunting.Branebjerg in 1996 and Larse have developed a kind of blender that repeats layering again.In micro-structural, because Reynolds number is very low, fluid often is laminar condition.If under the situation that does not influence fluid properties, two kinds of fluids are mixed by " overlapping " or " superposition ".Through after n such mixing, fluid-mixing is divided into the 2n layer.So just increased the contact-making surface between fluid greatly, mixed effect is greatly improved.1997, A.Desai etc. developed a kind of T type blender, and being applied to Reynolds number is the inferior Millisecond liquid mixing of 2000-6000.Center dies is made up of two T type blenders, and the middle microchannel connection of passing through one as reaction chamber enters the 3rd pipeline after liquid converges, form T font shape once more, to reach the even mixing of liquid.Document " principle and the making of PDMS film electromagnetism Micropump " (white orchid, Wu Yihui, Zhang Ping, Wang Shurong, Micrometer-Nanometer Processing Technology, 2003 the 2nd phases, the 54-58 page or leaf, in June, 2003) a kind of structure and operation principle of PDMS film electromagnetism Micropump are disclosed: the elasticity pumping diaphragm that PDMS film electromagnetism Micropump mainly is made of PDMS, Nd-Fe-B permanent magnetic approaches piece, and planar coil three parts of bottom constitute, when in planar coil, feeding the alternating current of square wave form, this coil can produce alternating magnetic field, magnetic field that the thin piece of Nd-Fe-B permanent magnetic forms and alternating magnetic field interact and drive the reciprocating motion of PDMS film, thereby cause the variation of pump chamber volume, and then the turnover of control micro fluid.
Detailed content of the present invention:
In sum, the microfluid static mixer uses under the Reynolds number condition with higher mostly, and mixing mechanism is still to be diffused as the master, can only cause chaos convection current among a small circle in local microchannel, incorporation time is long, speed is slow, and the laminar flow phenomenon is difficult for fundamentally solving.And injection device is generally external, can't be integrated.Dynamically be blended in and still can realize under the lower situation of Reynolds number fast, mix uniformly, but often complex structure is unfavorable for integrated making.Long at incorporation time in the above-mentioned background technology, speed slow, complex structure, integrated level are poor, the problem that adopts silicon sheet material cost height, bio-compatibility difference, the present invention will provide microfluid dynamic mixer, mixing pit mould and driving method thereof in the chip.
The microfluid dynamic mixer comprises in the chip of the present invention: mixing pit 1, first collimater 2, first optical fiber 3, position substrate 4, support 5, vibration film 6, AC power 7, planar coil 8, permanent magnetism thin slice 9, second optical fiber 10 and second collimater 11, the groove center fixation of support 5 has planar coil 8, the input of planar coil 8 is connected with AC power 7, high spot at support 5 is fixed with position substrate 4, its underpart was fixed on the position substrate 4 after first collimater 2 and second collimater 11 were coupled and aligned, the lower plane of mixing pit 1 is fixedlyed connected with the last plane of position substrate 4, first collimater 2 and second collimater, 11 its tops embed in the mixing pit 1, be fixedly connected with first optical fiber 3 and second optical fiber 10 respectively at the two ends of first collimater 2 and second collimater 11, fixedly connected with the top of vibration film 6 in the bottom of mixing pit 1, fixedly connected with the top of permanent magnetism thin slice 9 in bottom at vibration film 6, the space is arranged at the top of the bottom of permanent magnetism thin slice 9 and planar coil 8.
Position substrate 4 comprises as shown in Figure 2: lining body 12, first groove 13, through hole 14 and second groove 15, center symmetry preparation has first groove 13, second groove 15 and a through hole 14 on lining body 12 bodies, in two planar ends of through hole 14 first groove 13 and second groove 15 is arranged respectively; First collimater 2 and second collimater, 11 its underparts are fixed on respectively in first groove 13 and second groove 15.
Mixing pit 1 comprises as shown in Figure 3: supporter 16, three-flute 17, hybrid chamber 18 and the 4th groove 19, preparation has three-flute 17, hybrid chamber 18 and the 4th groove 19 on supporter 16 bodies, two planar ends at hybrid chamber 18 have three-flute 17 and the 4th groove 19 respectively, between hybrid chamber 18 and three-flute 17 and the 4th groove 19 wall of thin supporter 16 as hybrid chamber 18 are arranged; First collimater 2 and second collimater, 11 its tops embed respectively in three-flute 17 and the 4th groove 19.
The open mould of mixing pit of the present invention as shown in Figure 4, comprise: groove 20, spacious die body 21, square column 22, groove 23, cylinder 24 and square column 25, symmetrical distribution groove 20, square column 22, groove 23, cylinder 24 on the body of spacious die body 21, square column 22, square column 25 are profiles of groove 17, groove 19, groove 20, groove 23 are profiles of supporter 16, gapped in the planar ends of the end of square column 22 and square column 25 and cylinder 24 is the inwall of hybrid chamber 18, worn-out die body 21 and cylinder 24 are by being threaded, and cylinder 24 is positioned at worn-out die body 21 centers.
As shown in Figure 5 and as shown in Figure 6: the convex concave mould of mixing pit of the present invention comprises: body of punch 26, cylinder 27, molding screw 28, draw-screw 29, molding screw 30, draw-screw 31, die body 32, groove 33, plane of orientation 34, square column 35, groove 36, bottom groove 37 and square column 38, preparation has cylinder 27 on body of punch 26 bodies, molding screw 28 and molding screw 30, molding screw 28 and molding screw 30 are symmetrically distributed on the body of punch 26, cylinder 27 is positioned at the center of body of punch 26, draw-screw 29, draw-screw 31 is fixedlyed connected with body of punch 26 respectively, and cylinder 27 correspondences the profile of hybrid chamber 18; Fluted 33, the plane of orientation 34 of preparation, square column 35, groove 36, bottom groove 37 and square column 38 on die body 32 bodies, the last plane of square column 35 and square column 38 and plane of orientation 34 are in one plane and be symmetrically distributed, bilateral symmetry at square column 35 and square column 38 is distributed with groove 33, groove 36, in the preparation of the center of groove 33 and groove 36 bottom groove 37 is arranged.
When the microfluid dynamic mixer was worked in the chip: first optical fiber 3 linked to each other with light source, second optical fiber 10 links to each other with spectrometer, in hybrid chamber, inject sample and reagent earlier, open AC power then, in planar coil, feed the electric current of square wave form, utilize planar coil and permanent magnetism thin slice to form magnetic field and alternating magnetic field interaction, drive vibration film and move back and forth the generation resonant drive, make the flow field produce periodic disturbance and be formed with that eddy flow is moving to make the volume of hybrid chamber cause variation, thereby realize the dynamic mixing of microfluid in the chip.
Advantage of the present invention: the present invention adopts the method for electromagnetic excitation standing wave, has solved that driving voltage height, complicated operation, incorporation time are long, speed waits problem slowly; The present invention adopts the mixing pit design that groove is arranged, and adopts the mode that patches, and collimater is built in the problem that has solved the integrated level difficulty in the groove.The present invention adopts open mould to have advantage simple in structure, that operation is few.The present invention adopts concave, convex mould mould that vibration film and mixing pit are structure as a whole, and has reduced operation, man-hour.Because the present invention has utilized mould to prepare microfluid dynamic mixer in the chip, then can adopt the polymeric material that bio-compatibility is good, price is low, solved the problem that background technology adopts silicon sheet material cost height, bio-compatibility difference.
Description of drawings:
Microfluid dynamic mixer schematic diagram in Fig. 1 chip of the present invention;
Fig. 2 position substrate of the present invention;
The mixing pit of microfluid dynamicizer in Fig. 3 chip of the present invention;
The mixing pit open mould of microfluid dynamic mixer in Fig. 4 chip of the present invention;
The mixing pit punch of microfluid dynamic mixer in Fig. 5 chip of the present invention;
The mixing pit die of microfluid dynamic mixer in Fig. 6 chip of the present invention;
The specific embodiment:
The microfluid dynamic mixer comprises: mixing pit 1, first collimater 2, first optical fiber 3, position substrate 4, support 5, vibration film 6, AC power 7, planar coil 8, permanent magnetism thin slice 9, second optical fiber 10 and second collimater 11 as shown in Figure 1 in the chip of the present invention.
Mixing pit 1 comprises as shown in Figure 3: three-flute 17, hybrid chamber 18 and the 4th groove 19 are arranged on supporter 16 bodies.Mixing pit 1 can adopt light transmission polymeric materials good, good springiness such as PDMS, epoxy resin to make with vibration film 6.Mixing pit 1 and vibration film 6 can adopt injection molding method.
Position substrate 4 comprises as shown in Figure 2: lining body 12, first groove 13, through hole 14 and second groove 15, through hole 14 has two parallel planes, links to each other with first groove 13, second groove 15 respectively.Position substrate 4 can adopt materials such as silicon, glass, metal and organic polymer to make.
With PDMS is example, takes by weighing the PDMS prepolymer earlier and mixes at 10: 1 by mass ratio with curing agent, quietly puts or is placed in the vacuum desiccator, till the bubble in mixture does not have fully.When the hybrid chamber 18 on supporter 16 bodies adopts through hole, adopt the mixing pit open mould of microfluid dynamic mixer in the chip as shown in Figure 4.PDMS is poured in the mixing pit open mould, and quiet putting treats that it is even, smooth in (if any bubble, be placed on the bubble of bleeding in the vacuum desiccator in mixture do not have till).On the mixing pit open mould, put a smooth interlayer, push down (noting not moving) with slide.With anchor clamps worn-out mould of mixing pit and slide are clamped, then it is moved into the high-temperature baking some time in the baking oven.After the baking and curing, the mixing pit figure on the open mould has just been transferred on the PDMS after the curing.PDMS is carefully stripped down from worn-out mould, and the margins of excision part has just obtained the PDMS mixing pit 1 when hybrid chamber 18 is through hole.The vibration film 6 that is stained with permanent magnetism thin slice 9 with handles such as PDMS, 502 glue sticks on the lower plane of mixing pit, notices that permanent magnetism thin slice 9 should be at the center of hybrid chamber 18.When the hybrid chamber 18 on supporter 16 bodies adopts blind hole, adopt mixing pit punch, the concave die of microfluid dynamic mixer in the chip shown in accompanying drawing 5,6.PDMS is poured in the mixing pit die, and quiet putting treats that it is even, smooth in (if any bubble, be placed on the bubble of bleeding in the vacuum desiccator in mixture do not have till).The mixing pit punch is inserted in the die,, notes not moving with plane of orientation 34 location.With anchor clamps that the concavo-convex die clamp of mixing pit is tight, then it is moved into the high-temperature baking some time in the baking oven.After the baking and curing, the mixing pit figure on the concavo-convex mould has just been transferred on the PDMS after the curing.Utilize draw- screw 29,31 concavo-convex mould separately earlier, notice firmly that evenly the PDMS after will solidifying again carefully strips down, the margins of excision part has just obtained the PDMS mixing pit 1 when hybrid chamber 18 is blind hole.Degree of tightness by the control anchor clamps can be regulated the bottom thickness of blind hole, and controlled range is 10 μ m-300 μ m.When anchor clamps were the most loose, when promptly clearance between punch and die was maximum, bottom thickness was 300 μ m to the maximum; When anchor clamps are the tightest, promptly clearance between punch and die hour, the bottom thickness minimum is 10 μ m; Anchor clamps control clearance between punch and die can obtain the bottom thickness of 100 μ m when 100 μ m.
Microfluid dynamic mixer driving method as shown in Figure 1 in the chip of the present invention: at first utilize AC power 7 to feed the electric current of square wave form in planar coil 8, make planar coil 8 produce alternating magnetic field, utilize permanent magnetism thin slice 9 to form magnetic field and alternating magnetic field interaction again, drive vibration film 6 and move back and forth the generation resonant drive, it is moving to make flow field generation periodic disturbance be formed with eddy flow, thereby forms strong convection and improve mixing velocity and efficient with this.
When hybrid chamber 18 was used through hole, the present invention can adopt open mould to realize the making of mixing pit, as shown in Figure 4, comprising: groove 20, spacious die body 21, square column 22, groove 23, cylinder 24 and square column 25, can adopt material such as metal to make.Cylinder 24 have two with square column 22, the parallel plane of square column 25 end faces, these two plane symmetry distribute, the other parts of the cross section periphery of cylinder 24 can adopt straight line or circular arc.Gapped between cylinder 24 and square column 22, the square column 25, i.e. the wall of hybrid chamber 18.Cylinder 24 and spacious die body 21 are by being threaded.
When hybrid chamber 1 is used blind hole, the present invention can adopt convex concave mould to realize the making of mixing pit, as shown in Figure 5 and as shown in Figure 6, comprise: body of punch 26, cylinder 27, molding screw 28, draw-screw 29, molding screw 30, draw-screw 31, die body 32, groove 33, plane of orientation 34, square column 35, groove 36, bottom groove 37 and square column 38, can adopt materials such as metal to make.Cylinder 27 have two with square column 35, the parallel plane of square column 38 end faces, these two plane symmetry distribute, the other parts of the cross section periphery of cylinder 27 can adopt straight line or circular arc.Bottom groove 37 have two with square column 35, the parallel plane of square column 38 end faces, these two plane symmetry distribute, the other parts of the cross section periphery of bottom groove 37 can adopt straight line or circular arc.
Claims (3)
1, microfluid dynamic mixer in the chip, comprise: first optical fiber (3), support (5), AC power (7), second optical fiber (10), it is characterized in that also comprising: mixing pit (1), first collimater (2), position substrate (4), vibration film (6), planar coil (8), permanent magnetism thin slice (9), second collimater (11), the groove center fixation of support (5) has planar coil (8), the input of planar coil (8) is connected with AC power (7), high spot at support (5) is fixed with position substrate (4), its underpart was fixed on the position substrate (4) after first collimater (2) and second collimater (11) were coupled and aligned, the lower plane of mixing pit (1) is fixedlyed connected with the last plane of position substrate (4), its top of first collimater (2) and second collimater (11) embeds in the mixing pit (1), be fixedly connected with first optical fiber (3) and second optical fiber (10) respectively at the two ends of first collimater (2) and second collimater (11), fixedly connected with the top of vibration film (6) in the bottom of mixing pit (1), fixedly connected with the top of permanent magnetism thin slice (9) in bottom at vibration film (6), the space is arranged at the top of the bottom of permanent magnetism thin slice (9) and planar coil (8); Mixing pit (1) comprising: supporter (16), three-flute (17), hybrid chamber (18) and the 4th groove (19), preparation has three-flute (17), hybrid chamber (18) and the 4th groove (19) on supporter (16) body, two planar ends at hybrid chamber (18) have three-flute (17), the 4th groove (19) respectively, and the wall of thin supporter (16) as hybrid chamber (18) arranged between hybrid chamber (18) and three-flute (17), the 4th groove (19); First collimater (2) and its top of second collimater (11) embed respectively in three-flute (17) and the 4th groove (19).
2, microfluid dynamic mixer in the chip according to claim 1, it is characterized in that: position substrate (4) comprising: lining body (12), first groove (13), through hole (14) and second groove (15), center symmetry preparation has first groove (13), second groove (15) and a through hole (14) on lining body (12) body, in two planar ends of through hole (14) first groove (13) and second groove (15) is arranged respectively; First collimater (2) is fixed on respectively in first groove (13) and second groove (15) with second collimater (11) its underpart.
3, microfluid dynamic mixer in the chip according to claim 1 is characterized in that: the hybrid chamber (18) on supporter (16) body adopts through hole or blind hole; When hybrid chamber (18) adopts through hole, the fixing vibration film (6) in the bottom of through hole; When hybrid chamber (18) adopted blind hole, the bottom thickness of blind hole was chosen as 10 μ m-300 μ m.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100110633A CN100427194C (en) | 2004-08-27 | 2004-08-27 | Microfluid dynamic mixer, mixing pond mould in chip and driving method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100110633A CN100427194C (en) | 2004-08-27 | 2004-08-27 | Microfluid dynamic mixer, mixing pond mould in chip and driving method thereof |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810127165XA Division CN101308135B (en) | 2004-08-27 | 2004-08-27 | Convex concave mould for manufacturing chip microfluid dynamic mixer mixing pool |
| CN2008101271664A Division CN101308136B (en) | 2004-08-27 | 2004-08-27 | Open mould for manufacturing chip microfluid dynamic mixer mixing pool |
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| CN1598589A CN1598589A (en) | 2005-03-23 |
| CN100427194C true CN100427194C (en) | 2008-10-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102861526A (en) * | 2012-09-17 | 2013-01-09 | 江苏大学 | Flexible magnetomotive micro-mixing method and device of micro-fluidic chip |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100562749C (en) * | 2005-07-20 | 2009-11-25 | 中国科学院上海微系统与信息技术研究所 | Preparation facilities of a kind of low flux micro array biochip and preparation method thereof |
| ATE500884T1 (en) * | 2007-05-15 | 2011-03-15 | Corning Inc | MICROFLUIDIC AND SELF-RELAYING OSCILLATING MIXERS AND DEVICES AND METHODS FOR USE THEREOF |
| US7628082B2 (en) * | 2007-06-25 | 2009-12-08 | Integrated Sensing Systems, Inc. | Microfluidic device and microtube therefor |
| CN101564659B (en) * | 2008-04-25 | 2012-07-18 | 鸿富锦精密工业（深圳）有限公司 | Microfluid mixing device |
| CN109999930B (en) * | 2019-04-04 | 2020-07-03 | 中国科学院长春光学精密机械与物理研究所 | Microfluid dynamic mixer and chip system thereof |
| CN111468018A (en) * | 2020-04-16 | 2020-07-31 | 西南交通大学 | Flexible active micro-mixer device integration system and preparation method |
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| CN2473618Y (en) * | 2001-04-20 | 2002-01-23 | 中国科学院长春光学精密机械与物理研究所 | Micro integrated biochemical analysis chip |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2473618Y (en) * | 2001-04-20 | 2002-01-23 | 中国科学院长春光学精密机械与物理研究所 | Micro integrated biochemical analysis chip |
Non-Patent Citations (2)
| Title |
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| PDMS 薄膜电磁微泵的原理与制作. 白兰，吴一辉，张平，王淑荣.微细加工技术，第2003年卷第2期. 2003 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102861526A (en) * | 2012-09-17 | 2013-01-09 | 江苏大学 | Flexible magnetomotive micro-mixing method and device of micro-fluidic chip |
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