CN100397062C - Apparatus for quick separation of pine wood nematode and detection method therefor - Google Patents
Apparatus for quick separation of pine wood nematode and detection method therefor Download PDFInfo
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- CN100397062C CN100397062C CNB2005100880623A CN200510088062A CN100397062C CN 100397062 C CN100397062 C CN 100397062C CN B2005100880623 A CNB2005100880623 A CN B2005100880623A CN 200510088062 A CN200510088062 A CN 200510088062A CN 100397062 C CN100397062 C CN 100397062C
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- suction cylinder
- plank
- hopper
- nematode
- wood
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Abstract
The present invention discloses a quick separation apparatus and a detection method thereof for pine wood nematode, which relates to a quick separation apparatus and a detection method thereof for pine wood nematode in wood, particularly to a quick separation apparatus and a detection method thereof for pine wood nematode in a wood board. The present invention provides a separation apparatus and a detection method thereof which are more suitable for the quarantine of packaging wood and cause nematode in a wood board to be taken out by water flow swimmingly for detection. A suction drum and a wood board presser of the separation apparatus are fixed on a supporting frame; the suction drum is a pipe body, a drum opening forms a blade shape, holes are arranged in the pipe wall near the drum opening, and the pipe wall near the drum opening is fixedly provided with a pipeline; the pipeline is connected with a vacuum pump; holes are arranged below the suction drum; the wood board presser is arranged above the suction drum, a pressing board of the wood board presser is connected with screw rods, and the screw rods are connected with hand wheels. During detection, a gap is arranged along the vertical direction of a wood fiber pipe in the center of a wood board sample, the gap faces to the suction drum, and the gap covers the drum opening. The wood board is embedded into the edges of the suction drum by rotating the hand wheels; the vacuum pump is started, water in a water storage tank flows into the suction drum; a power supply is cut off, the water in the suction drum flows into a culture dish, and the nematode is detected below an anatomical lens.
Description
Technical field
The present invention relates to timber, particularly a kind of fast separation device and the detection method thereof of the pine wood nematode in the plank.
Background technology
Pine wood nematode (Bursaphelenchus xylophilus) can cause pine tree wilt disease, because this disease is by insect-borne transmissions such as longicorns, and pathogenic nematode history of life is short, reproductive capacity is strong, therefore spreads very soon and causes disaster, and is called as " smokeless forest fire " or " pine tree cancer ".This disease does not still have practicable methods of treatment at present, forbids that it is one of major measure that prevents this disease diffusion that lesion timber moves.In addition,, therefore, strengthen quarantine, comprise that internal quarantine and external quarantine also are to prevent one of major measure of this disease diffusion because of the packing case of store of goods is made of pine.Interior inspection is that the inspection post is set up on main traffic main artery in various places, interception pine tree diseased wood; Outer inspection is a Wooden packing container of tightly checking cargoes imported and exported at each port.But, from timber, isolate nematode and also identified, be a time-consuming loaded down with trivial details job of taking a lot of work.Usually must be split into little (also thinner) earlier after the timber sampling, be placed on then in the containers such as funnel or large beaker, separate, generally need just can isolate nematode through 24h by the graceful funnel ratio juris of Bel than chopsticks.(Jian Yongxun etc. such as Jian Yongxun, the research of pine wood nematode quarantine and examination sampling method, the sick worm of Chinese forest, 2001,4:5-6,23) though report to adopt electric drill to drill through wood chip more laborsaving than splitting head, separation method is not broken away from " the graceful funnel of Bel ", therefore still needs just can go out the result more than the 20h.In order to solve this difficult problem, " fast separation device of pine material nematode and the detection method thereof " of inventor's invention (CN02129944.7) makes the time of separating nematode from timber shorten to 20～30min from 24h.But this device is applicable to the inspection round log, to checking the not very good usefulness of plank; And in real work, the quarantine amount of wooden packing is also more than the quarantine amount of round log.
Summary of the invention
Purpose of the present invention aims to provide a kind of wooden current that quarantine, that the nematode quilt in the plank is passed through rapidly of packing that more are applicable to and takes tripping device and the detection method of being identified thereof smoothly out of.
The tripping device of the said a kind of pine wood nematode of the present invention is provided with suction cylinder, hopper, vacuum pump, plank pressure applicator and fixed support, suction cylinder and plank pressure applicator are individually fixed on the fixed support, suction cylinder is that a bottom is funnelform body or horizontal columniform body or curved shape body, the diameter of suction cylinder is 4～10cm, height is 10～20cm, the nozzle of suction cylinder is knife edge shape edge, the nearly nozzle tube wall of suction cylinder is provided with an aperture and sets firmly a pipeline, as aspirating hole, pipeline connects vacuum pump, suction cylinder below is established an aperture in addition, as the setting-out hole and set firmly valve; The plank pressure applicator is located at the suction cylinder top, and the plank pressure applicator comprises pressure plate, screw rod and handwheel, and pressure plate is connected with screw rod, catcher wheel on the screw rod.
Hopper can fuse with suction cylinder, and when the two was one, hopper was positioned at the top of suction cylinder, and its diameter is 2.5～4 times of suction cylinder diameter, highly is 1/2～1/3 of suction cylinder height; The top of suction cylinder exceeds the bottom surface 0.5～1.5cm of hopper, seals water-tight between the two.
In addition, enter vacuum pump, can between vacuum pump and suction cylinder, be connected a bottle,suction that drying agent is housed for preventing steam.
The present invention is said, and to utilize the tripping device of above-mentioned a kind of pine wood nematode to detect the method for pine wood nematode as follows:
1) saw is got one section plank sample, and along sawing out or dig out breach with the vertical direction of timber dimension pipe, the degree of depth of breach is no more than the thickness of plank (promptly can not penetrate plank) in plank sample central authorities;
2) the plank breach towards suction cylinder and cover on the nozzle of suction cylinder, when hopper and suction cylinder are one, plank to be checked is all in hopper, if hopper and suction cylinder are split, then the lower end of plank to be checked should be stretched in the hopper below suction cylinder:
3) rotate handwheel, rely on the strength of screw rod precession, plank is embedded in the edge of suction cylinder knife edge shape;
4) clear water is poured in the hopper, allowed water logging not have plank;
5) start vacuum pump, the water in the hopper will flow in the suction cylinder;
6) close the power supply of vacuum pump, open the valve in setting-out hole, suction cylinder below, allow the water in the suction cylinder flow in the double dish, check that under anatomical lens nematode is (if water is relatively more muddy, available clear water reexamines after precipitating 1～2 time), or further under high-power microscope or inverted microscope, identify the kind of nematode.
The present invention more is applicable to the quarantine of packing wood, it can isolate hiding nematode in timber, particularly in plank, isolate hiding nematode, not only make the above mask work of original 24h of need be able within 10～20min, finish, be convenient to the Rapid identification pine wood nematode, improve the efficient of quarantine and examination work, accelerated wooden packing goods and the Wood products card speed of being open to the custom greatly.Also exempted simultaneously the hardship that quarantine and examination personnel splitting is chipped.
Description of drawings
Fig. 1 is the fast separation device structural representation that the bottom is the funnel-form body for suction cylinder.
Fig. 2 is the fast separation device structural representation of horizontal cylindrical tube for suction cylinder.
Fig. 3 is the fast separation device structural representation of suction cylinder for curved shape body.
Its body embodiment
As shown in Figure 1, the fast separation device of pine wood nematode comprises suction cylinder 1, hopper 2, vacuum pump, plank pressure applicator 3 and fixed support 4, suction cylinder and plank pressure applicator are individually fixed on the fixed support, suction cylinder is that a bottom is funnelform body, its internal diameter 5.5cm, external diameter 6cm, high 15cm.Be drilled with an aperture and a bit of tubule 11 of burn-oning is made aspirating hole on the tube wall at 1cm place, distance top, the external diameter of tubule is 1cm, can be inserted in pressure-resistant plastic tube and link to each other with vacuum pump.The nozzle of suction cylinder is ground to knife edge shape edge.Add a hopper 2 on the table top above the suction cylinder, its diameter is 20cm, highly is 6cm.The nozzle of suction cylinder (top) exceeds the bottom surface 1.0cm of hopper, and is airtight and water-tight between the two.Plank pressure applicator 3 is located at the suction cylinder top, comprises pressure plate 31, and pressure plate connects a screw rod 32, connects a handwheel 33 on the screw rod.Steam enters vacuum pump when preventing to bleed, and can be connected a bottle,suction that drying agent is housed between vacuum pump and suction cylinder.
Saw is got one section plank sample, and its length and width are greater than the suction cylinder diameter, less than the diameter of hopper.In plank sample central authorities, along sawing out breach with the vertical direction of timber dimension pipe, the degree of depth of breach is no more than the thickness of plank with circular saw.The plank tipping that cutter is good is come, make breach towards lower cover on the nozzle of suction cylinder.Clockwise direction is rotated handwheel 33, and plank is embedded in the edge of suction cylinder knife edge shape.Clear water is poured in the hopper, and the water surface exceeds plank 1.0cm (flooding plank).Start vacuum pump, the water in the hopper just flows in the suction cylinder of plank below.The power supply of turning off vacuum pump, open the valve 12 in setting-out hole, suction cylinder below, allow the water in the suction cylinder flow in the double dish, under anatomical lens, check to have or not nematode, if what nematode does not all have, the goods and the delivery vehicle thereof that just can allow this batch pack with timber are let pass; If nematode is arranged, then draw several nematodes, be contained in covered on the microslide, be placed on and identify it is what nematode under the high-power microscope; If pine wood nematode is arranged, then will destroy or fumigation.
Referring to Fig. 2, similar to Example 1, what they were different is that suction cylinder is horizontal cylindrical tube, and with whole device half-twist placement, above the suction cylinder away from tube port position and below respectively get out an aperture and burn-on a bit of tubule 11 and 12 on the tube wall of nearly tube port position, the former links to each other with vacuum pump by aspirating hole, and the latter is the setting-out hole, and suction cylinder and hopper independently are provided with.During detection one of plank sample to be checked end (upper end) is sealed with the paraffin or the Instant cement of hot melt, the other end (lower end) immerses in the water.All the other are with embodiment 1.
Referring to Fig. 3, suction cylinder is changed into curved shape body, the invariant position of aspirating hole, but the setting-out hole is located at the bottom of bend pipe.
Claims (4)
1. the tripping device of a pine wood nematode, it is characterized in that being provided with suction cylinder, hopper, vacuum pump, plank pressure applicator and fixed support, suction cylinder and plank pressure applicator are individually fixed on the fixed support, suction cylinder is that a bottom is funnelform body or horizontal columniform body or curved shape body, the diameter of suction cylinder is 4～10cm, height is 10～20cm, the nozzle of suction cylinder is knife edge shape edge, the nearly nozzle tube wall of suction cylinder is provided with a hole as aspirating hole and set firmly a pipeline, pipeline connects vacuum pump, suction cylinder below is established a hole in addition, as the setting-out hole and set firmly valve; The plank pressure applicator is located at the suction cylinder top, and the plank pressure applicator comprises pressure plate, screw rod and handwheel, and pressure plate is connected with screw rod, catcher wheel on the screw rod; Hopper and suction cylinder fuse, and hopper is positioned at the top of suction cylinder, and its diameter is 2.5～4 times of suction cylinder diameter, highly are 1/2～1/3 of suction cylinder height.
2. the tripping device of a kind of pine wood nematode as claimed in claim 1 is characterized in that the top of suction cylinder exceeds the bottom surface 0.5～1.5cm of hopper.
3. the tripping device of a kind of pine wood nematode as claimed in claim 1 is characterized in that being connected a bottle,suction that drying agent is housed between vacuum pump and suction cylinder.
4. use the method for the tripping device detection pine wood nematode of the described a kind of pine wood nematode of claim 1, it is characterized in that the steps include:
1) saw is got one section plank sample, and along establishing breach with the vertical direction of timber dimension pipe, the degree of depth of breach is less than the thickness of plank in plank sample central authorities;
2) the plank breach towards suction cylinder and cover on the nozzle of suction cylinder, when hopper and suction cylinder were one, plank to be checked was all in hopper; Or
If hopper and suction cylinder are split, then the lower end of plank to be checked should be stretched in the hopper below suction cylinder;
3) rotate handwheel, rely on the strength of screw rod precession, plank is embedded in the edge of suction cylinder knife edge shape;
4) clear water is poured in the hopper, allowed water logging not have plank;
5) start vacuum pump, the water in the hopper flows in the suction cylinder;
6) close the power supply of vacuum pump, open the valve in setting-out hole, suction cylinder below, allow the water in the suction cylinder flow into double dish, under anatomical lens, check nematode, or further under high-power microscope or inverted microscope, identify the kind of nematode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100880623A CN100397062C (en) | 2005-08-01 | 2005-08-01 | Apparatus for quick separation of pine wood nematode and detection method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100880623A CN100397062C (en) | 2005-08-01 | 2005-08-01 | Apparatus for quick separation of pine wood nematode and detection method therefor |
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| Publication Number | Publication Date |
|---|---|
| CN1731130A CN1731130A (en) | 2006-02-08 |
| CN100397062C true CN100397062C (en) | 2008-06-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2005100880623A Expired - Fee Related CN100397062C (en) | 2005-08-01 | 2005-08-01 | Apparatus for quick separation of pine wood nematode and detection method therefor |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101832889B (en) * | 2010-04-30 | 2011-11-23 | 王进福 | Sheet washing method for rapid pine wood nematode separation technology |
| CN104412954B (en) * | 2013-08-22 | 2016-12-28 | 天津卫技天马除害处理技术服务有限公司 | The quarantining treatment system and method for nematicide in a kind of flowers kind ball |
| CN107356459B (en) * | 2017-06-20 | 2020-09-15 | 潘沧桑 | Detachable and washable fast pine wood nematode separating device and detection method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB295126A (en) * | 1927-08-09 | 1928-08-09 | Adolf Martin Kobiolke | Process and apparatus for preserving timber and other materials and the destruction of insect and other pests therein |
| US4027401A (en) * | 1975-06-23 | 1977-06-07 | Fairbanks Jr John B | Method and structure for curing and/or treating wood |
| CN1019366B (en) * | 1985-04-01 | 1992-12-09 | 罗森伦德 | Method for impregnating wood and its apparatus |
| CN1184479C (en) * | 2003-01-21 | 2005-01-12 | 厦门大学 | Fast separation device of pine material nematode and its detection method |
-
2005
- 2005-08-01 CN CNB2005100880623A patent/CN100397062C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB295126A (en) * | 1927-08-09 | 1928-08-09 | Adolf Martin Kobiolke | Process and apparatus for preserving timber and other materials and the destruction of insect and other pests therein |
| US4027401A (en) * | 1975-06-23 | 1977-06-07 | Fairbanks Jr John B | Method and structure for curing and/or treating wood |
| CN1019366B (en) * | 1985-04-01 | 1992-12-09 | 罗森伦德 | Method for impregnating wood and its apparatus |
| CN1184479C (en) * | 2003-01-21 | 2005-01-12 | 厦门大学 | Fast separation device of pine material nematode and its detection method |
Non-Patent Citations (2)
| Title |
|---|
| 线虫分离器的改进和松材线虫分离特点. 周弘等.植物检疫，第16卷第4期. 2002 |
| 线虫分离器的改进和松材线虫分离特点. 周弘等.植物检疫，第16卷第4期. 2002 * |
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| CN1731130A (en) | 2006-02-08 |
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Owner name: PAN CANGSANG Free format text: FORMER OWNER: XIAMEN UNIVERSITY Effective date: 20140124 |
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Effective date of registration: 20140124 Address after: Xiamen University East Siming District of Xiamen city in Fujian province 361005 Building No. 5 Room 202 Patentee after: Pan Cangsang Address before: Xiamen City, Fujian Province, 361005 South Siming Road No. 422 Patentee before: Xiamen University |
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