CN100361630C - Diode laser spectrum instrument for tumor imaging and diagnosis - Google Patents
Diode laser spectrum instrument for tumor imaging and diagnosis Download PDFInfo
- Publication number
- CN100361630C CN100361630C CNB2005101273632A CN200510127363A CN100361630C CN 100361630 C CN100361630 C CN 100361630C CN B2005101273632 A CNB2005101273632 A CN B2005101273632A CN 200510127363 A CN200510127363 A CN 200510127363A CN 100361630 C CN100361630 C CN 100361630C
- Authority
- CN
- China
- Prior art keywords
- diode laser
- interferometric filter
- fluorescence
- laser
- convex lens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention discloses a diode laser spectrum instrument for tumor imaging and diagnosis, which relates to an instrument for tumor imaging and diagnosis. The present invention is to solve the problem how to use laser to induce photosensitizer ALA to generate a fluorescence spectrum to make tumors image. The present invention utilizes laser with 405 nms of wave length output by a diode laser (1), and the laser which is expanded in beam is irradiated on the human organism (6) on which the photosensitizer ALA is injected; the laser excites the autofluorescence of the human organism and the fluorescence of the injected photosensitizer to return to the surface of a dichromatism directive mirror (3) along a light path, and the fluorescence is reflected by the dichromatism directive mirror (3) and penetrates a first interference filter (7) and a second interference filter (8) successively; a CCD detector (9) receives the fluorescence penetrating the first interference filter (7) and the second interference filter (8) successively, and a microcomputer (10) which is connected with the CCD detector (9) displays the image of the detected human organism. The instrument of the present invention can predicate the situation of the detected organism by observing the color of each point of the organism, and then judges the organism whether has tumors and the malignant degree. The present invention has low cost and convenient carryover.
Description
Technical field
The present invention relates to a kind of instrument for tumor imaging and diagnosis device.
Background technology
Diagnosing tumor method at present commonly used has imaging examination (X line, CT, magnetic resonance), B ultrasonic, positron emission tomography art (PET), biochemistry method (enzyme, hormone, receptor etc.), cytology and pathological diagnosis, gene diagnosis etc., but these means just seem powerless to the early diagnosis of cancer and minimum cancer and even precancerous lesion.Photodynamic therapy is the novel medical skill that is used for diagnosing tumor and treatment that grows up the eighties.Photodynamic therapy is that the laser by photosensitizer and specific wavelength causes biological photosensitization and reacts and damage the purpose that tumor and other pathological proliferation reach treatment, and it has Wicresoft or noinvasive, have no side effect, do not damage many distinct advantages such as function of human body; Photodynamics diagnosis is a kind of contactless diagnosis that matches with photodynamic therapy, it have diagnosis accurate, can determine the malignization degree of tumor boundaries, tumor and the characteristics such as photodynamic therapy effect of cancer.Photodynamic therapy has been obtained achievement highly visible since coming out, can be used for treating malignant tumor, part benign tumor, precancerous lesion and the viral disease of almost all kinds.At present, photodynamic therapy has been subjected to the extensive concern of Chinese scholars and medical circle, all obtain government permission in many developed countries such as the U.S., Canada, Holland, Germany, Japan, and be widely used in treatment pulmonary carcinoma, the esophageal carcinoma, bladder cancer, skin carcinoma, colorectal cancer, laryngeal carcinoma, oral cancer etc.And to make PDT bring into play its medical function better, just be necessary for it diagnostic data accurately is provided.
At present, along with development of high-tech such as laser technology, optical fiber technology, CCD Detection Techniques, the fluorescence spectrum diagnostic techniques has obtained strong development, particularly recent years in a lot of fields, and this technology is proved aspect detection and differentiation malignant tumor and non-malignant tumors very potential.Some researcheres have brought into use the autofluorescence of tissue and photosensitizer fluorescence that colorectal cancer, the early stage of lung cancer, gastric cancer, breast carcinoma, bladder cancer and skin carcinoma etc. are diagnosed, the result shows, quick and objective, sensitive and accurate, painless characteristics such as harmless and simple and practical that laser Induced Fluorescence Technology has, can diagnose out sciagraphy, cytolgical examination, white light prop up organ pipe spectroscopy and CT technology the infantile tumour that can not survey and navigate to.At present, three kinds of photosensitive drugs (Photofrin, ALA, Visudyne) have obtained FDA Food and Drug Administration (FDA) approval list marketing.Having only Britain DIOMED630 type light power laser therapeutic apparatus at present is unique light power armarium through drugs approved by FDA.The photosensitizer that this photodynamic therapeutic instrument is suitable for is Photofrin.And photosensitizer nitrilo acetic acid propanoic acid (being ALA) enjoys domestic and international medical circle to welcome with short advantage of its metabolism time.Yet also there are not photodynamic therapeutic instrument and diagnostic apparatus merchandise sales both at home and abroad at photosensitizer ALA.And,, carried out certain research work though the hospital and the research center of some universities are arranged for light power diagnosis, device therefor costs an arm and a leg, relatively backward, and do not see have product to come out.In view of external development and domestic wilderness demand space, research and development advanced person's light power diagnosis equipment is imperative.Publication number is that the patent of CN1543914A can come tumor is carried out a diagnosis by fluorescence spectrum, judges its deterioration degree, but but can't realize the imaging of malignant tumor, and is more directly perceived, reproduce the deterioration degree of tumor visually.
Summary of the invention
How to utilize laser induced photosensitizer ALA to produce the problem that fluorescence spectrum makes tumor imaging in order solving, to the invention provides a kind of diode laser spectrum instrument for tumor imaging and diagnosis.Instrument of the present invention as light source, utilizes laser-induced tissue fluorescence and photosensitizer ALA characteristic fluorescence to the tested tissue imaging with diode laser, comes the character of diagnosing tumour, discovery to hide focus by the gained image, and determines its border; This instrument not only can provide experimental basis for the early diagnosis of cancer, for PDT provides reliable therapeutic domain, and help to determine the excision extension of tumor in the operation, checks whether excision is thorough, for following up a case by regular visits to behind the oncotherapy provides important referential data.
Diode laser spectrum instrument for tumor imaging and diagnosis of the present invention comprises diode laser, first convex lens, microcomputer and ccd detector, as excitation source, the laser of diode laser output changes directional light into after by first convex lens with diode laser; It is characterized in that described diagnostic apparatus comprises that also two colors are to mirror, second convex lens, the 3rd convex lens, first interferometric filter, second interferometric filter and motor, the wavelength of described diode laser output is 405nm (nanometer), the described directional light of exporting from the light output end of first convex lens passes two colors to mirror, shining injection from two colors after the laser of mirror output expands bundle by second convex lens and the 3rd convex lens has on the tissue of photosensitizer ALA, and described two colors are used for reflected fluorescent light, transmission laser to mirror; Fluorescence by the photosensitizer ALA of the autofluorescence of the tissue of laser excitation and injection turns back to the surface of two colors to mirror through second convex lens and the 3rd convex lens, described two kinds of fluorescence are also seen through first interferometric filter and second interferometric filter by two colors respectively to mirroring, first interferometric filter is fixedlyed connected with the output shaft of motor with second interferometric filter, and described motor drives first interferometric filter and second interferometric filter is that turning cylinder rotates in same horizontal plane with this output shaft, the autofluorescence of the described first interferometric filter transmission tissue, photosensitizer fluoresces in the described second interferometric filter transmission tissue; Described ccd detector receives the fluorescence that sees through first interferometric filter earlier, and then receives the fluorescence of second interferometric filter, is shown the image of tested person soma by the microcomputer that is connected with ccd detector.
Operation principle: with the excitation source of a diode laser as photosensitizer, the laser that is produced 405nm by diode laser at first becomes directional light through lens, this directional light shines the tissue of injection (smear or oral) photosensitizer ALA after mirror and one group of beam expanding lens through two colors, tissue and inner photosensitizer thereof will send peak value respectively at the fluorescence of 470nm and 635nm under the inducing of 405nm laser, institute fluoresces and can return along original optical path, fluorescence successively enters in the ccd detector at second interferometric filter of 635nm at first interferometric filter and the central homology wavelength of 470nm through the central homology wavelength after mirroring through two colors, and the computer that be connected with ccd detector this moment will store the 470nm that tested tissue each point that CCD monitors sends and the fluorescence intensity level of 635nm wavelength.Two interferometric filters are by step motor control, and when first interferometric filter was in the light path, CCD surveys was the fluorescence intensity of the 470nm that sends of tested tissue each point, is stored in the computer; When second interferometric filter was in the light path, what CCD surveyed was the fluorescence intensity of the 635nm that photosensitizer sends in the tissue, is stored in the computer.After computer is handled, the wavelength of organizing each point to send is the fluorescence intensity of 635nm and the corresponding different color of ratio of the fluorescence intensity of 470nm, press the yellowish green Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) purple of the corresponding successively reddish orange of ratio size, the tested tissue each point just is presented on the computer screen with different colors like this.Can conclude the situation of tested tissue by the color relation of tissues observed each point, judge that whether it is tumor and malignization degree, then can provide its concrete border for the tumor after making a definite diagnosis, as shown in Figure 3.
Device of the present invention has the following advantages: 1, the present invention adopts diode laser, make that device of the present invention is light, be easy to carry about with one, this for make house calls, many section office utilize jointly same set of equipment provide convenience and may; 2, its diagnosis rapid sensitive can repeat, but record can utilize image to carry out the pathological changes location, can be group discussion objective data is provided; 3, in diagnostic procedure, patient's noinvasive, painless; 4, it can combine with endoscope, is used for contactless diagnosis in the body: 5, instrument use of the present invention is wide, economical and practical, is easy to promote; Advantages such as 6, instrument cost of the present invention is low, and is painless harmless, simple and practical are for three early (early find, early diagnosis, early treatment) of realizing cancer provides a kind of new approach.
Description of drawings
Fig. 1 is a system structure sketch map of the present invention; Fig. 2 is the syndeton sketch map of first interferometric filter 7 and second interferometric filter 8 and motor 11 in the instrument of the present invention; Fig. 3 is a testing result sketch map of the present invention.
The specific embodiment
In conjunction with Fig. 1 and Fig. 2 this specific embodiment is described, this specific embodiment is made up of to mirror 3, second convex lens 4, the 3rd convex lens 5, first interferometric filter 7, second interferometric filter 8, motor 11, microcomputer 10 and ccd detector 9 diode laser 1, first convex lens 2, two colors, as excitation source, the laser of diode laser 1 output changes directional light into after by first convex lens 2 with diode laser 1; The wavelength of described diode laser 1 output is 405nm, the described directional light of exporting from the light output end of first convex lens 2 passes two colors to mirror 3, shining injection from two colors after the laser of mirror 3 outputs expands bundle by second convex lens 4 and the 3rd convex lens 5 has on the tissue 6 of photosensitizer ALA, and described two colors are used for reflected fluorescent light, transmission laser to mirror 3; Fluorescence by the photosensitizer ALA of the autofluorescence of the tissue of laser excitation and injection turns back to the surface of two colors to mirror 3 through second convex lens 4 and the 3rd convex lens 5, described two kinds of fluorescence by two colors to mirror 3 reflection and see through first interferometric filter 7 and second interferometric filter 8 respectively, first interferometric filter 7 is fixedlyed connected with the output shaft 11-1 of motor 11 with second interferometric filter 8, and described motor 11 drives first interferometric filter 7 and second interferometric filter 8 is that turning cylinder rotates in same horizontal plane with this output shaft 11-1, the autofluorescence of described first interferometric filter, 7 transmission tissues, photosensitizer fluoresces in described second interferometric filter, the 9 transmission tissues; Described ccd detector 9 receives the fluorescence that sees through first interferometric filter 7 earlier, adds the fluorescence that receives second interferometric filter 8 then, is shown the image of tested person soma by the microcomputer 10 that is connected with ccd detector 9.Described two colors are used for the fluorescence of reflection wavelength greater than 405nm to mirror 3, and transmission peak wavelength is the laser of 405nm; Described first interferometric filter 7 is central homology wavelength bandpass filters at 470nm, half-peak breadth 12nm; Described second interferometric filter 8 is central homology wavelength bandpass filters at 635nm, half-peak breadth 12nm; The power of described diode laser 1 output is 10～30mw; The resolution of described ccd detector 9 is 1600 * 1200; The temperature of described diode laser 1 is controlled by temperature controller, and its temperature-control range is 8～60 ℃, and temperature-controlled precision is 0.1 ℃; The input current of described diode laser 1 is controlled by current controller, and its span of control is 0～200mA, and control accuracy is 0.1mA; The rotating speed of motor can be regulated as required, but must guarantee that the inversion frequency of two optical filters (7 and 8) is less than the sensitization frequency of ccd detector 9.Fig. 3 has shown the result schematic diagram that this specific embodiment detects, and zones of different has represented lesion degree from heavily to gently from the redness to the purple.
Claims (6)
1. diode laser spectrum instrument for tumor imaging and diagnosis, described diagnostic apparatus comprises diode laser (1), first convex lens (2), microcomputer (10) and ccd detector (9), as excitation source, the laser of diode laser (1) output changes directional light into after by first convex lens (2) with diode laser (1); It is characterized in that described diagnostic apparatus comprises that also two colors are to mirror (3), second convex lens (4), the 3rd convex lens (5), first interferometric filter (7), second interferometric filter (8) and motor (11), the wavelength of described diode laser (1) output is 405nm, the described directional light of exporting from the light output end of first convex lens (2) passes two colors to mirror (3), shining injection from two colors after the laser of mirror (3) output expands bundle by second convex lens (4) and the 3rd convex lens (5) has on the tissue (6) of photosensitizer ALA, and described two colors are used for reflected fluorescent light to mirror (3), transmission laser; Fluorescence by the photosensitizer ALA of the autofluorescence of the tissue of laser excitation and injection turns back to the surface of two colors to mirror (3) through second convex lens (4) and the 3rd convex lens (5), described two kinds of fluorescence by two colors to mirror (3) reflection and see through first interferometric filter (7) and second interferometric filter (8) respectively, first interferometric filter (7) is fixedlyed connected with the output shaft (11-1) of motor (11) with second interferometric filter (8), and described motor (11) drives first interferometric filter (7) and second interferometric filter (8) is that turning cylinder rotates in same horizontal plane with this output shaft (11-1), the autofluorescence of described first interferometric filter (7) transmission tissue, photosensitizer fluoresces in described second interferometric filter (8) the transmission tissue; Described ccd detector (9) receives the fluorescence that sees through first interferometric filter (7) earlier, and then receive the fluorescence of second interferometric filter (8), the image that shows tested person soma by the microcomputer (10) that is connected with ccd detector (9), the wavelength of organizing each point to send is the fluorescence intensity of 635nm and the corresponding different color of ratio of the fluorescence intensity of 470nm, press the yellowish green Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) purple of the corresponding successively reddish orange of ratio size, the tested tissue each point just is presented on the computer screen with different colors like this, the situation of the color relation decidable tested tissue by the tissues observed each point, judge that whether it is tumor and malignization degree, then can provide its concrete border for the tumor after making a definite diagnosis.
2. diode laser spectrum instrument for tumor imaging and diagnosis according to claim 1 is characterized in that described two colors are used for the fluorescence of reflection wavelength greater than 405nm to mirror (3), and transmission peak wavelength is the laser of 405nm.
3. diode laser spectrum instrument for tumor imaging and diagnosis according to claim 1 is characterized in that described first interferometric filter (7) is the bandpass filter of central homology wavelength at 470nm, half-peak breadth 12nm.
4. diode laser spectrum instrument for tumor imaging and diagnosis according to claim 1 is characterized in that described second interferometric filter (8) is the bandpass filter of central homology wavelength at 635nm, half-peak breadth 12nm.
5. diode laser spectrum instrument for tumor imaging and diagnosis according to claim 1 is characterized in that the power of described diode laser (1) output is 10～30mW.
6. diode laser spectrum instrument for tumor imaging and diagnosis according to claim 1, the resolution that it is characterized in that described CCD survey meter (9) is 1600 * 1200.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101273632A CN100361630C (en) | 2005-12-20 | 2005-12-20 | Diode laser spectrum instrument for tumor imaging and diagnosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101273632A CN100361630C (en) | 2005-12-20 | 2005-12-20 | Diode laser spectrum instrument for tumor imaging and diagnosis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1803096A CN1803096A (en) | 2006-07-19 |
| CN100361630C true CN100361630C (en) | 2008-01-16 |
Family
ID=36865341
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101273632A Expired - Fee Related CN100361630C (en) | 2005-12-20 | 2005-12-20 | Diode laser spectrum instrument for tumor imaging and diagnosis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100361630C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008122628A (en) | 2006-11-10 | 2008-05-29 | Fuji Xerox Co Ltd | Vertical fringe interference pattern projection lens |
| AU2016260153B2 (en) * | 2015-05-08 | 2019-04-04 | Jiangsu Kunhui Biotechnology Co., Ltd. | Method for detecting, in noninvasive in-vivo manner, skin damage induced by ultraviolet light, and detection device thereof |
| CN106420041A (en) * | 2015-08-13 | 2017-02-22 | 复旦大学附属肿瘤医院 | High frequency electric operating knife with fiber probe |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999022814A1 (en) * | 1997-10-30 | 1999-05-14 | Esc Medical Systems Ltd. | System and method for endoscopically applying and monitoring photodynamic therapy and photodynamic diagnosis |
| US6026319A (en) * | 1997-02-13 | 2000-02-15 | Fuji Photo Film Co., Ltd. | Fluorescence detecting system |
| US6212425B1 (en) * | 1995-09-26 | 2001-04-03 | Karl Storz Gmbh & Co., Kg | Apparatus for photodynamic diagnosis |
| US20020035330A1 (en) * | 2000-07-14 | 2002-03-21 | Xillix Technologies Corporation | Compact fluorescent endoscopy video system |
| CN1543914A (en) * | 2003-11-10 | 2004-11-10 | 哈尔滨工业大学 | Laser spectrum cancer diagnostic device with diode laser |
-
2005
- 2005-12-20 CN CNB2005101273632A patent/CN100361630C/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6212425B1 (en) * | 1995-09-26 | 2001-04-03 | Karl Storz Gmbh & Co., Kg | Apparatus for photodynamic diagnosis |
| US6026319A (en) * | 1997-02-13 | 2000-02-15 | Fuji Photo Film Co., Ltd. | Fluorescence detecting system |
| WO1999022814A1 (en) * | 1997-10-30 | 1999-05-14 | Esc Medical Systems Ltd. | System and method for endoscopically applying and monitoring photodynamic therapy and photodynamic diagnosis |
| US20020035330A1 (en) * | 2000-07-14 | 2002-03-21 | Xillix Technologies Corporation | Compact fluorescent endoscopy video system |
| CN1543914A (en) * | 2003-11-10 | 2004-11-10 | 哈尔滨工业大学 | Laser spectrum cancer diagnostic device with diode laser |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1803096A (en) | 2006-07-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yang et al. | A multispectral fluorescence imaging system: Design and initial clinical tests in intra‐operative Photofrin‐photodynamic therapy of brain tumors | |
| AU2002228673B9 (en) | Fluorescence-mediated molecular tomography | |
| EP0513986B1 (en) | A photosensitizer enhanced fluorescence biopsy needle | |
| US8865128B2 (en) | Folate targeted enhanced tumor and folate receptor positive tissue optical imaging technology | |
| Kondepati et al. | Recent applications of near-infrared spectroscopy in cancer diagnosis and therapy | |
| Andersson‐Engels et al. | Preliminary evaluation of two fluorescence imaging methods for the detection and the delineation of basal cell carcinomas of the skin | |
| US20110042580A1 (en) | Fluorescence quantification and image acquisition in highly turbid media | |
| US8858914B2 (en) | Folate targeted enhanced tumor and folate receptor positive tissue optical imaging technology | |
| CN201578365U (en) | Surgical oncology intraoperative fluorescent navigation system | |
| CN113614486A (en) | Systems, methods, and apparatus for three-dimensional imaging, measurement, and display of wound and tissue specimens | |
| CN100361630C (en) | Diode laser spectrum instrument for tumor imaging and diagnosis | |
| CN1256918C (en) | Laser spectrum cancer diagnostic device with diode laser | |
| Zharkova et al. | Laser-excited fluorescence spectrometric system for tissue diagnostics | |
| WO2009005549A2 (en) | Angiogenesis monitoring using in vivo hyperspectral radiometric imaging | |
| Gibbs-Strauss et al. | Noninvasive measurement of aminolevulinic acid-induced protoporphyrin IX fluorescence allowing detection of murine glioma in vivo | |
| Kustov et al. | Intraoperative video-fluorescence navigation by PpIX and tissue saturation measurement during surgical resection of gastric malignant tumor | |
| Borisova et al. | Diagnostics of Pigmented Skin Tumors Based on Light-Induced Autofluorescence and Diffuse Reflectance Spectroscopy | |
| Pfefer et al. | Long‐term Effects of Photodynamic Therapy on Fluorescence Spectroscopy in the Human Esophagus¶ | |
| Pogue et al. | Fluorescent molecular imaging and dosimetry tools in photodynamic therapy | |
| Gordon et al. | Doppler optical coherence tomography for monitoring the vascular effects of photodynamic therapy | |
| Dalal et al. | Characterization Of Breast Cancer Tissue Using Autofluorescence And Fluorescence Spectroscopy In-Vitro | |
| Mathurb | Optical diagnostic and therapeutic technologies in pulmonary medicine | |
| Patil et al. | 18 Autofluorescence-Guided Resection of Intracranial Tumor | |
| Meyers | Optical Detection of Cancer | |
| Vasefi et al. | Autofluorescence-Guided Resection of Intracranial Tumor: Past, Present, and Future |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |