CA2090904A1 - Dna detection method - Google Patents
Dna detection methodInfo
- Publication number
- CA2090904A1 CA2090904A1 CA002090904A CA2090904A CA2090904A1 CA 2090904 A1 CA2090904 A1 CA 2090904A1 CA 002090904 A CA002090904 A CA 002090904A CA 2090904 A CA2090904 A CA 2090904A CA 2090904 A1 CA2090904 A1 CA 2090904A1
- Authority
- CA
- Canada
- Prior art keywords
- primer
- selfcomplementary
- disclosed
- region
- detection method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A process for the detection of a nucleic acid sequence in a homogeneous assay format using an energy transfer system is disclosed. This process utilizes a 5' labeled primer containing a selfcomplementary sequence in an amplification process together with a subsequent detection step using a 3' labeled probe for the amplified region. The labels will be close together in space after hybridizing the probe dose to the short piece of double-stranded DNA
resulting from backfolding of the selfcomplementary region of the primer which has been incorporated into the amplified product. The new primer for use in this process is also disclosed.
resulting from backfolding of the selfcomplementary region of the primer which has been incorporated into the amplified product. The new primer for use in this process is also disclosed.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP92104956A EP0566751B1 (en) | 1992-03-23 | 1992-03-23 | DNA detection method |
EP92104956.5 | 1992-03-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2090904A1 true CA2090904A1 (en) | 1993-09-24 |
CA2090904C CA2090904C (en) | 2004-08-10 |
Family
ID=8209457
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002090904A Expired - Lifetime CA2090904C (en) | 1992-03-23 | 1993-03-03 | Dna detection method |
Country Status (9)
Country | Link |
---|---|
US (1) | US5573906A (en) |
EP (1) | EP0566751B1 (en) |
JP (1) | JP3771281B2 (en) |
CA (1) | CA2090904C (en) |
DE (1) | DE69207580T2 (en) |
DK (1) | DK0566751T3 (en) |
ES (1) | ES2082256T3 (en) |
GR (1) | GR3019617T3 (en) |
IE (1) | IE72498B1 (en) |
Cited By (21)
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US5591578A (en) * | 1993-12-10 | 1997-01-07 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5770369A (en) * | 1993-12-10 | 1998-06-23 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5952172A (en) * | 1993-12-10 | 1999-09-14 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6013170A (en) * | 1997-06-12 | 2000-01-11 | Clinical Micro Sensors, Inc. | Detection of analytes using reorganization energy |
US6071699A (en) * | 1996-06-07 | 2000-06-06 | California Institute Of Technology | Nucleic acid mediated electron transfer |
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US6096273A (en) * | 1996-11-05 | 2000-08-01 | Clinical Micro Sensors | Electrodes linked via conductive oligomers to nucleic acids |
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US7935481B1 (en) | 1999-07-26 | 2011-05-03 | Osmetech Technology Inc. | Sequence determination of nucleic acids using electronic detection |
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US7273749B1 (en) | 1990-06-04 | 2007-09-25 | University Of Utah Research Foundation | Container for carrying out and monitoring biological processes |
US5935522A (en) * | 1990-06-04 | 1999-08-10 | University Of Utah Research Foundation | On-line DNA analysis system with rapid thermal cycling |
US7081226B1 (en) | 1996-06-04 | 2006-07-25 | University Of Utah Research Foundation | System and method for fluorescence monitoring |
US6576419B1 (en) * | 1993-07-23 | 2003-06-10 | University Of Utah Research Foundation | Assay procedure using fluorogenic tracers |
US5620850A (en) | 1994-09-26 | 1997-04-15 | President And Fellows Of Harvard College | Molecular recognition at surfaces derivatized with self-assembled monolayers |
DK1179600T3 (en) | 1996-06-04 | 2005-09-05 | Univ Utah Res Found | Hybridization monitoring during PCR |
ES2354902T3 (en) * | 1996-06-04 | 2011-03-21 | University Of Utah Research Foundation | APPARATUS TO CARRY OUT THE PCR AND MONITORING OF THE REACTION IN REAL TIME DURING TEMPERATURE CYCLES. |
AU726821B2 (en) * | 1996-07-16 | 2000-11-23 | Gen-Probe Incorporated | Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm |
US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US7070925B1 (en) | 1996-07-16 | 2006-07-04 | Gen-Probe Incorporated | Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe |
US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
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US6312902B1 (en) | 1998-03-13 | 2001-11-06 | Promega Corporation | Nucleic acid detection |
US6277578B1 (en) | 1998-03-13 | 2001-08-21 | Promega Corporation | Deploymerization method for nucleic acid detection of an amplified nucleic acid target |
US6270973B1 (en) | 1998-03-13 | 2001-08-07 | Promega Corporation | Multiplex method for nucleic acid detection |
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FR2565996B1 (en) * | 1984-06-15 | 1988-01-22 | Inst Nat Sante Rech Med | CHEMICALLY MARKED NUCLEIC ACIDS, THEIR USE AND THE NECESSARY FOR ITS IMPLEMENTATION |
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-
1992
- 1992-03-23 DE DE69207580T patent/DE69207580T2/en not_active Expired - Lifetime
- 1992-03-23 ES ES92104956T patent/ES2082256T3/en not_active Expired - Lifetime
- 1992-03-23 DK DK92104956.5T patent/DK0566751T3/en active
- 1992-03-23 EP EP92104956A patent/EP0566751B1/en not_active Expired - Lifetime
-
1993
- 1993-03-03 CA CA002090904A patent/CA2090904C/en not_active Expired - Lifetime
- 1993-03-22 IE IE930219A patent/IE72498B1/en not_active IP Right Cessation
- 1993-03-22 JP JP06153893A patent/JP3771281B2/en not_active Expired - Lifetime
- 1993-03-22 US US08/035,010 patent/US5573906A/en not_active Expired - Lifetime
-
1996
- 1996-04-09 GR GR960401012T patent/GR3019617T3/en unknown
Cited By (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6277576B1 (en) | 1993-12-10 | 2001-08-21 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6238870B1 (en) | 1993-12-10 | 2001-05-29 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5770369A (en) * | 1993-12-10 | 1998-06-23 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5780234A (en) * | 1993-12-10 | 1998-07-14 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5824473A (en) * | 1993-12-10 | 1998-10-20 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5952172A (en) * | 1993-12-10 | 1999-09-14 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5705348A (en) * | 1993-12-10 | 1998-01-06 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6528266B2 (en) | 1993-12-10 | 2003-03-04 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6268149B1 (en) | 1993-12-10 | 2001-07-31 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6087100A (en) * | 1993-12-10 | 2000-07-11 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6268150B1 (en) | 1993-12-10 | 2001-07-31 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5591578A (en) * | 1993-12-10 | 1997-01-07 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6177250B1 (en) | 1993-12-10 | 2001-01-23 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6180352B1 (en) | 1993-12-10 | 2001-01-30 | California Insitute Of Technology | Nucleic acid mediated electron transfer |
US6200761B1 (en) | 1993-12-10 | 2001-03-13 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US6258545B1 (en) | 1993-12-10 | 2001-07-10 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US7033760B2 (en) | 1995-06-07 | 2006-04-25 | California Institute Of Technology | Metallic solid supports modified with nucleic acids |
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Also Published As
Publication number | Publication date |
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US5573906A (en) | 1996-11-12 |
DE69207580T2 (en) | 1996-08-08 |
GR3019617T3 (en) | 1996-07-31 |
DE69207580D1 (en) | 1996-02-22 |
DK0566751T3 (en) | 1996-03-04 |
ES2082256T3 (en) | 1996-03-16 |
CA2090904C (en) | 2004-08-10 |
EP0566751A1 (en) | 1993-10-27 |
JP3771281B2 (en) | 2006-04-26 |
IE72498B1 (en) | 1997-04-23 |
EP0566751B1 (en) | 1996-01-10 |
JPH067199A (en) | 1994-01-18 |
IE930219A1 (en) | 1993-10-06 |
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