BRPI0921966A2 - levedura expressando celulases para sacarificação e fermentação simultâneas usando celulose. - Google Patents

levedura expressando celulases para sacarificação e fermentação simultâneas usando celulose.

Info

Publication number
BRPI0921966A2
BRPI0921966A2 BRPI0921966A BRPI0921966A BRPI0921966A2 BR PI0921966 A2 BRPI0921966 A2 BR PI0921966A2 BR PI0921966 A BRPI0921966 A BR PI0921966A BR PI0921966 A BRPI0921966 A BR PI0921966A BR PI0921966 A2 BRPI0921966 A2 BR PI0921966A2
Authority
BR
Brazil
Prior art keywords
fermentation
cellulose
simultaneous saccharification
yeast expressing
expressing cellulases
Prior art date
Application number
BRPI0921966A
Other languages
English (en)
Inventor
Froelich Alan
Gilbert Alan
K Warner Anne
Rice Charles
Ghandi Chhayal
Murphy Dan
Lagrange Danie
Willes Deidre
Brevnova Elena
Van Zyl Emile
A Stonehouse Emilly
Xu Haowen
Hanson Heidi
Shikare Indraneel
Uusitalo Jaana
Villari Jeff
D Keating Jeffrey
Flatt Jim
Mcbrid John
Deleault Kristen
Ilmen Marja
Mellon Mark
Siika-Aho Matti
Penttila Merja
Thorngren Naomi
Den Haan Riaan
Rose Shaunita
Rajaharia Vineet
Original Assignee
Mascoma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mascoma Corp filed Critical Mascoma Corp
Publication of BRPI0921966A2 publication Critical patent/BRPI0921966A2/pt
Publication of BRPI0921966A8 publication Critical patent/BRPI0921966A8/pt
Publication of BRPI0921966B1 publication Critical patent/BRPI0921966B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0921966-8A 2008-11-21 2009-11-23 Célula hospedeira de levedura termotolerante, método de fermentar celulose e cultura BRPI0921966B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11698108P 2008-11-21 2008-11-21
US61/116,981 2008-11-21
PCT/US2009/065571 WO2010060056A2 (en) 2008-11-21 2009-11-23 Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

Publications (3)

Publication Number Publication Date
BRPI0921966A2 true BRPI0921966A2 (pt) 2017-10-24
BRPI0921966A8 BRPI0921966A8 (pt) 2017-12-12
BRPI0921966B1 BRPI0921966B1 (pt) 2019-05-07

Family

ID=42198846

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0921966-8A BRPI0921966B1 (pt) 2008-11-21 2009-11-23 Célula hospedeira de levedura termotolerante, método de fermentar celulose e cultura

Country Status (10)

Country Link
US (4) US9102955B2 (pt)
EP (1) EP2361311B1 (pt)
CN (2) CN108315272A (pt)
AU (1) AU2009316309B2 (pt)
BR (1) BRPI0921966B1 (pt)
CA (3) CA3092340A1 (pt)
DK (1) DK2361311T3 (pt)
ES (1) ES2621181T3 (pt)
NZ (1) NZ593469A (pt)
WO (1) WO2010060056A2 (pt)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009139839A1 (en) 2008-05-11 2009-11-19 Mascoma Corporation Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases
ES2621181T3 (es) * 2008-11-21 2017-07-03 Lallemand Hungary Liquidity Management Llc Levaduras que expresan celulasas para la sacarificación y fermentación simultáneas utilizando celulosa
BR122018013596B1 (pt) 2009-10-26 2024-01-30 Universiteit Stellenbosch (Also Known As Stellenbosch University) Células hospedeiras de saccharomyces cerevisiae bem como processos para hidrólise de um substrato celulósico e para fermentação de celulose
US20130071884A1 (en) 2009-11-06 2013-03-21 Agrivida, Inc. Plants expressing cell wall degrading enzymes and expression vectors
US9605269B2 (en) 2010-05-05 2017-03-28 Lallemand Hungary Liquidity Management Llc Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
US20130323822A1 (en) * 2010-06-03 2013-12-05 Mascoma Corporation Yeast Expressing Saccharolytic Enzymes for Consolidated Bioprocessing Using Starch and Cellulose
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
PL2468875T3 (pl) * 2010-12-22 2022-11-07 Neste Oyj Zintegrowany sposób wytwarzania biopaliw
DK3235903T3 (da) 2011-01-26 2021-10-11 Novozymes Inc Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem
MX2013007720A (es) 2011-01-26 2013-08-09 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
WO2012122308A2 (en) * 2011-03-07 2012-09-13 Agrivida, Inc. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes
CA2832279C (en) 2011-04-05 2024-03-05 Mascoma Corporation Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
US9598689B2 (en) 2011-11-10 2017-03-21 Lallemand Hungary Liquidity Management Llc Genetically modified strain of S. cerevisiae engineered to ferment xylose and arabinose
US9708591B2 (en) 2011-11-18 2017-07-18 Novozymes Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
AU2012352763A1 (en) * 2011-12-13 2014-04-24 Danisco Us Inc. Enzyme cocktails prepared from mixed cultures
DK2794664T3 (en) * 2011-12-22 2017-08-21 Novozymes Inc HYBRID POLYPEPTIDES WITH CELLOBIO HYDROLASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
JP2013169154A (ja) * 2012-02-17 2013-09-02 Toyota Motor Corp セルロース系バイオマス分解増強活性ポリペプチド
US20140024064A1 (en) 2012-07-23 2014-01-23 Butamax(Tm) Advanced Biofuels Llc Processes and systems for the production of fermentative alcohols
CN102851324A (zh) * 2012-08-24 2013-01-02 太仓市周氏化学品有限公司 一种利用耐高温酵母同步糖化发酵产乙醇的方法
AU2013315520B2 (en) 2012-09-12 2017-03-30 Butamax Advanced Biofuels Llc Processes and systems for the production of fermentation products
AU2013329024A1 (en) 2012-10-11 2015-03-12 Butamax Advanced Biofuels Llc Processes and systems for the production of fermentation products
BR112015009237A2 (pt) * 2012-10-31 2017-08-22 Danisco Us Inc Composição compreendendo beta-glicosidase de magnaporthe grisea, ácido nucleico, célula hospedeira e métodos para produzir beta-glicosidase e para hidrolisar substrato de biomassa lignocelulósica
BR112015009201A2 (pt) * 2012-10-31 2017-11-14 Danisco Us Inc composição compreendeendo um polipeptídeo recombinante, ácido nucleico codificando o mesmo, célula hospedeira, bem como métodos para produzir uma beta-glucosidase e para hidrolisar um substrato de biomassa lignocelulósica
BR112015010134A2 (pt) 2012-11-09 2017-08-22 Lallemand Hungary Liquidity Man Llc Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicas
BR112015013647A2 (pt) 2012-12-12 2017-11-14 Danisco Us Inc variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
CA2905033C (en) * 2013-03-15 2023-06-27 Lallemand Hungary Liquidity Management Llc Expression of beta-glucosidases for hydrolysis of lignocellulose and associated oligomers
CN103409333B (zh) * 2013-05-20 2015-01-28 山东大学 一株持续高效分泌β-葡萄糖苷酶的重组酿酒酵母菌株及其应用
SI3008193T1 (en) * 2013-06-12 2018-08-31 Renescience A/S Methods for treating urban solid waste (MTO) using microbial hydrolysis and fermentation and liquid slurry, which is the result of
CN105814191A (zh) 2013-08-15 2016-07-27 拉勒曼德匈牙利流动管理有限责任公司 通过甘油回收改进微生物中的产物产率和生产的方法
WO2015042543A2 (en) * 2013-09-20 2015-03-26 The Trustees Of Columbia University In The City Of New York Biofuel production enzymes and uses thereof
DK3083671T3 (da) 2013-12-20 2020-12-07 Hutchinson Fred Cancer Res Mærkede kimære effektormolekyler og receptorer deraf
CN103805673B (zh) * 2014-03-01 2015-12-09 青岛科技大学 一种利用转基因酵母混合发酵生产秸秆乙醇的方法
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
CN104046605A (zh) * 2014-05-29 2014-09-17 中国科学院广州能源研究所 一种嗜温耐乙醇β-葡萄糖苷酶及其编码基因和应用
WO2016037096A1 (en) 2014-09-05 2016-03-10 Novozymes A/S Carbohydrate binding module variants and polynucleotides encoding same
DK3191587T3 (da) 2014-09-08 2019-10-14 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
CN104357340B (zh) * 2014-11-17 2017-08-01 中国食品发酵工业研究院 一种生物转化富马酸生产l‑苹果酸的方法
BR112017018030A2 (pt) 2015-02-24 2018-04-10 Novozymes A/S variante de celobiohidrolase, polinucleotídeo isolado, célula hospedeira recombinante, método para produção de uma variante de celobiohidrolase, composição de enzima inteiro ou composição de cultura de células, e, processos para degradação de um material celulósico, para produção de um produto de fermentação e para fermentação de um material celulósico
EP3288570A4 (en) 2015-04-29 2018-11-21 Fred Hutchinson Cancer Research Center Modified stem cells and uses thereof
EP3288569A4 (en) * 2015-04-29 2018-12-19 Fred Hutchinson Cancer Research Center Modified hematopoietic stem/progenitor and non-t effector cells, and uses thereof
CN106086087A (zh) * 2016-08-18 2016-11-09 吉林中之林农业科技有限公司 一种微生物共培养制备生物质单体的系统
BR122022015725B1 (pt) 2017-05-23 2023-03-14 Lallemand Hungary Liquidity Management Llc Processo para a produção de um produto de fermentação
EP3502126A1 (en) * 2017-12-19 2019-06-26 CIC nanoGUNE - Asociación Centro de Investigación Cooperativa en Nanociencias Ancestral cellulases and uses thereof
CN108707510B (zh) * 2018-06-07 2021-07-06 湖北中烟工业有限责任公司 一种红枣烟草复合美拉德反应物的制备方法及其应用
CN108823102B (zh) * 2018-06-10 2021-09-03 东北农业大学 寒地秸秆腐熟真菌被孢霉菌株及其在水稻秸秆腐熟中的应用
CN109593662B (zh) * 2018-12-11 2021-12-03 大连理工大学 一种乙酸和木糖共利用的马克斯克鲁维酵母菌株及筛选方法
CN109981620A (zh) * 2019-03-14 2019-07-05 山东浪潮云信息技术有限公司 一种后台接口管理系统
CN110438019B (zh) * 2019-06-06 2021-04-06 浙江工业大学 一种复合菌剂及其发酵餐厨垃圾制备有机液体肥料的应用
CN112852649B (zh) * 2019-11-28 2022-08-23 华东理工大学 一株耐高温的生产纤维素乙醇的酿酒酵母菌株及其发酵应用
CN112899294B (zh) * 2021-02-04 2022-07-22 温氏食品集团股份有限公司 一种多功能酶基因hg27及应用
WO2024097990A1 (en) * 2022-11-03 2024-05-10 Xylome Corporation Carbohydrolytic and lipogenic recombinant yeasts

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9400623D0 (en) * 1994-01-14 1994-03-09 Univ Leeds Exploitation of the cellulase enzyme complex of neurospora
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
JP3645394B2 (ja) 1997-03-12 2005-05-11 日立建機株式会社 タイヤローラ
US7026152B2 (en) * 2000-06-26 2006-04-11 University Of Florida Research Foundation, Inc. Methods and compositions for simultaneous saccharification and fermentation
US7045331B2 (en) * 2001-12-18 2006-05-16 Genencor International, Inc. EGVII endoglucanase and nucleic acids encoding the same
US7344876B2 (en) * 2003-01-24 2008-03-18 Phage Biotechnology, Inc. Kluyveromyces strains metabolizing cellulosic and hemicellulosic materials
DK1626979T3 (da) * 2003-05-02 2012-07-09 Cargill Inc Genetisk modificeret gærart og fremgangsmåder til fermentering, hvor den genetisk modificerede gær anvendes
WO2005116271A2 (en) 2004-05-25 2005-12-08 The Trustees Of Dartmouth College Selection of microorganisms with growth dependent upon extracytoplasmic enzymes
ES2607891T3 (es) * 2004-07-16 2017-04-04 Dsm Ip Assets B.V. Ingeniería metabólica de células eucariotas que fermentan xilosa
WO2008008793A2 (en) 2006-07-10 2008-01-17 Dyadic International Inc. Methods and compositions for degradation of lignocellulosic material
EP2099923A2 (en) * 2006-11-22 2009-09-16 The Trustees of Dartmouth College Recombinant yeast strains expressing tethered cellulase enzymes
WO2008155665A2 (en) 2007-05-09 2008-12-24 University Of Stellenbosch Method for enhancing cellobiose utilization
US7923236B2 (en) 2007-08-02 2011-04-12 Dyadic International (Usa), Inc. Fungal enzymes
WO2009139839A1 (en) 2008-05-11 2009-11-19 Mascoma Corporation Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases
CA3021166C (en) 2008-05-11 2024-01-09 Universiteit Stellenbosch Heterologous expression of fungal cellobiohydrolases in yeast
CA2729945A1 (en) 2008-07-07 2010-01-14 Mascoma Corporation Heterologous expression of termite cellulases in yeast
WO2010005553A1 (en) 2008-07-07 2010-01-14 Mascoma Corporation Isolation and characterization of schizochytrium aggregatum cellobiohydrolase i (cbh 1)
ES2621181T3 (es) 2008-11-21 2017-07-03 Lallemand Hungary Liquidity Management Llc Levaduras que expresan celulasas para la sacarificación y fermentación simultáneas utilizando celulosa
US9206434B2 (en) 2008-12-23 2015-12-08 Enchi Corporation Heterologous biomass degrading enzyme expression in thermoanaerobacterium saccharolyticum
US9315833B2 (en) 2009-02-20 2016-04-19 Lallemand Hungary Liquidity Management Llc Yeast cells expressing an exogenous cellulosome and methods of using the same
US20120295306A1 (en) 2009-04-21 2012-11-22 The Trustees Of Dartmouth College Modified CIPA Gene From Clostridium Thermocellum for Enhanced Genetic Stability
WO2011022651A1 (en) 2009-08-21 2011-02-24 John Mcbride Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms
US9605269B2 (en) 2010-05-05 2017-03-28 Lallemand Hungary Liquidity Management Llc Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
US20130323822A1 (en) 2010-06-03 2013-12-05 Mascoma Corporation Yeast Expressing Saccharolytic Enzymes for Consolidated Bioprocessing Using Starch and Cellulose
FI20105632A0 (fi) * 2010-06-04 2010-06-04 Valtion Teknillinen Menetelmä proteiinien tuottamiseksi rihmamaisilla sienillä
CA2881772C (en) 2012-08-29 2023-03-21 Lallemand Hungary Liquidity Management Llc Expression of enzymes in yeast for lignocellulose derived oligomer cbp
CA2905033C (en) 2013-03-15 2023-06-27 Lallemand Hungary Liquidity Management Llc Expression of beta-glucosidases for hydrolysis of lignocellulose and associated oligomers

Also Published As

Publication number Publication date
EP2361311B1 (en) 2017-01-18
WO2010060056A3 (en) 2010-07-15
US9102955B2 (en) 2015-08-11
CA2964245C (en) 2020-10-27
ES2621181T3 (es) 2017-07-03
CA2744495C (en) 2017-05-30
US20160010117A1 (en) 2016-01-14
CN108315272A (zh) 2018-07-24
DK2361311T3 (en) 2017-04-24
US20120129229A1 (en) 2012-05-24
AU2009316309A1 (en) 2011-10-20
WO2010060056A2 (en) 2010-05-27
BRPI0921966B1 (pt) 2019-05-07
CA3092340A1 (en) 2010-05-27
EP2361311A4 (en) 2013-03-27
EP2361311A2 (en) 2011-08-31
US20210388398A1 (en) 2021-12-16
CA2964245A1 (en) 2010-05-27
NZ593469A (en) 2012-12-21
CN102272303A (zh) 2011-12-07
US9988652B2 (en) 2018-06-05
BRPI0921966A8 (pt) 2017-12-12
CA2744495A1 (en) 2010-05-27
AU2009316309B2 (en) 2016-07-07
US20180258449A1 (en) 2018-09-13

Similar Documents

Publication Publication Date Title
BRPI0921966A2 (pt) levedura expressando celulases para sacarificação e fermentação simultâneas usando celulose.
BRPI0921438A2 (pt) Hospedeiros e processos de fermentação para produção de celulase
BRPI0820497A2 (pt) Processo para produção de etanol utilizando celulose com enzimas geradas através de cultura do estado sólido.
BRPI0915828A2 (pt) expressão heteróloga de celulases de cupim em levedura
EP2173889A4 (en) SIMULTANEOUS SUGARING AND FERMENTATION OF FIBER-CONTAINING BIOMASS
BRPI0908198A2 (pt) Célula de fermentação de açúcar pentose
BRPI1011597A2 (pt) Métodos para degradar ou converter um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico, e, composição de enzima.
BRPI0923915A2 (pt) endoglucanases fúngicas, sua produção e uso
BRPI0922612A2 (pt) microorganismo expressando xilose isomerase
BRPI0905853A2 (pt) Meios de fermentação e processos destes.
BRPI0913400A2 (pt) método para produzir ácido graxos para biocombustível, biodiesel e outros produtos químicos valiosos
BRPI0910992A2 (pt) método simplificado para digestão de biomassa celulósica
BRPI0905792A2 (pt) método de liquefação aprimorada e sacarificação de biomassa lignocelulósica
BRPI0812267A2 (pt) Célula hospedeira fúngica filamentosa, método para produzir uma composição de proteína celulolítica, composição de proteína celulolítica, e, métodos para degradar ou converter um material, contendo celulose e para produzir um produto de fermentação
BRPI0923026A2 (pt) métodos para degradar ou converter um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico.
ZA201101167B (en) Increased heterologous fe-s enzyme activity in yeast
BRPI1009093A2 (pt) "método para pré-tratamento do estoque de alimentação de biomassa celulósica que compreende celulose, hemicelulose, e lignina".
BRPI1008370A2 (pt) Biomassa sacarificante
BRPI0914208A2 (pt) célula de eletrólise, e, método
BRPI0923646A2 (pt) Processo de biotecnologia microbiana para degradar completamente glúten em farinhas.
BR112012032504A2 (pt) método para desconstruir e extraír moléculas de açúcar de biomassa que compreende celulose e hemicelulose; material de biomassa desconstruída, hidrosilado condicionado; método para produzir um bioproduto, método para desconstruir biomassa que compreende celulose e hemicelulose para a extração de moléculas de açúcar da dita biomassa e material de biomassa
WO2009158627A3 (en) Cellulosic protein expression in yeast
BRPI0920891A2 (pt) misturas de alfa-amilase e métodos para uso das referidas misturas
BRPI1013889A2 (pt) Célula de levedura, e, método para gerar uma célula de levedura.
BR112014004193A2 (pt) célula hospedeira recombinante de trichoderma, métodos para produzir uma composição enzimática, composição enzimática, processos para degradar um material celulósico, para sintetizar um produto de fermentação e para fermentar um material celulósico

Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC. (HU)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 23/11/2009, OBSERVADAS AS CONDICOES LEGAIS. (CO) 20 (VINTE) ANOS CONTADOS A PARTIR DE 23/11/2009, OBSERVADAS AS CONDICOES LEGAIS

B25G Requested change of headquarter approved

Owner name: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC. (HU)