BR9916840A - Integrated portable biological detection system - Google Patents
Integrated portable biological detection systemInfo
- Publication number
- BR9916840A BR9916840A BR9916840-5A BR9916840A BR9916840A BR 9916840 A BR9916840 A BR 9916840A BR 9916840 A BR9916840 A BR 9916840A BR 9916840 A BR9916840 A BR 9916840A
- Authority
- BR
- Brazil
- Prior art keywords
- integrated portable
- detection system
- dna
- biological detection
- followed
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 2
- 230000004544 DNA amplification Effects 0.000 abstract 1
- 239000003298 DNA probe Substances 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000004720 dielectrophoresis Methods 0.000 abstract 1
- 239000000975 dye Substances 0.000 abstract 1
- 239000007850 fluorescent dye Substances 0.000 abstract 1
- 238000012632 fluorescent imaging Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000002032 lab-on-a-chip Methods 0.000 abstract 1
- 230000002093 peripheral effect Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Dispersion Chemistry (AREA)
- Signal Processing (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Electrostatic Separation (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Patente de Invenção: <B>"SISTEMA DE DETECçãO BIOLóGICA PORTáTIL INTEGRADO"<D>. A presente invenção refere-se a separação de células bacterianas e cancerígenas de sangue humano periférico em chips eletrónicos microfabricados por dieletroforese. As células isoladas foram examinadas por coloração dos núcleos com corante fluorescente, seguida por formação de imagem fluorescente induzida por laser. DNA e RNA também foram liberados eletronicamente das células liberadas, e as sequências de marcadores específicos detectadas por amplificação de DNA, seguida por hibridização eletrónica para as sondas de captura imobilizadas. As tentativas no sentido da construção de um sistema "lab-on-a chip" são apresentadas, o que envolve a seleção de sondas de DNA, corantes, reagentes e estabelecimento de um protótipo do instrumento portátil completamente integrado.Invention Patent: <B> "INTEGRATED PORTABLE BIOLOGICAL DETECTION SYSTEM" <D>. The present invention relates to the separation of bacterial and cancer cells from peripheral human blood into electronic chips manufactured by dielectrophoresis. Isolated cells were examined by staining the nuclei with fluorescent dye, followed by laser-induced fluorescent imaging. DNA and RNA were also released electronically from the released cells, and the sequences of specific markers detected by DNA amplification, followed by electronic hybridization for the immobilized capture probes. Attempts to build a "lab-on-a chip" system are presented, which involves selecting DNA probes, dyes, reagents and establishing a fully integrated portable instrument prototype.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11373098P | 1998-12-23 | 1998-12-23 | |
PCT/US1999/031098 WO2000037163A1 (en) | 1998-12-23 | 1999-12-22 | Integrated portable biological detection system |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9916840A true BR9916840A (en) | 2001-10-09 |
Family
ID=22351155
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9916840-5A BR9916840A (en) | 1998-12-23 | 1999-12-22 | Integrated portable biological detection system |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1144092A4 (en) |
JP (1) | JP2002536962A (en) |
KR (1) | KR20010102961A (en) |
AU (1) | AU763514B2 (en) |
BR (1) | BR9916840A (en) |
CA (1) | CA2353461A1 (en) |
NZ (1) | NZ512087A (en) |
WO (1) | WO2000037163A1 (en) |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6379897B1 (en) | 2000-11-09 | 2002-04-30 | Nanogen, Inc. | Methods for gene expression monitoring on electronic microarrays |
CN1181337C (en) | 2000-08-08 | 2004-12-22 | 清华大学 | Solid molecule operating method in microfluid system |
US6884395B2 (en) | 2000-05-12 | 2005-04-26 | Gyros Ab | Integrated microfluidic disc |
US6277582B1 (en) * | 2000-07-27 | 2001-08-21 | Becton, Dickinson And Company | Amplification and detection of mycoplasma pneumoniae targeting the P1 gene |
US6261785B1 (en) * | 2000-07-27 | 2001-07-17 | Becton, Dickinson And Company | Amplification and detection of Bordetella pertussis |
US6251609B1 (en) * | 2000-07-27 | 2001-06-26 | Becton, Dickinson And Company | Amplification and detection of Legionella pneumophila targeting the mip gene |
CN100495030C (en) | 2000-09-30 | 2009-06-03 | 清华大学 | Multi-force operator and use thereof |
JP4505776B2 (en) * | 2001-01-19 | 2010-07-21 | 凸版印刷株式会社 | Gene detection system, gene detection apparatus equipped with the same, detection method, and gene detection chip |
KR100393131B1 (en) * | 2001-03-15 | 2003-07-31 | 케이맥(주) | Method for crystalizing proteins in nano scale, and chamber for crystalizing proteins and chip comprising the same |
US6890409B2 (en) | 2001-08-24 | 2005-05-10 | Applera Corporation | Bubble-free and pressure-generating electrodes for electrophoretic and electroosmotic devices |
GB0205455D0 (en) | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
AU2003218806A1 (en) * | 2002-03-27 | 2003-10-08 | Jae-Chern Yoo | Bio-disc, bio-driver apparatus, and assay method using the same |
EP1517752A4 (en) * | 2002-05-03 | 2009-07-08 | Univ California | Fast electrical lysis of cells and rapid collection of the contents thereof using capillary electrophoresis |
KR100441893B1 (en) * | 2002-08-03 | 2004-07-27 | 학교법인 포항공과대학교 | Nucleic acid separation device using MEMS component |
JP4216018B2 (en) * | 2002-08-26 | 2009-01-28 | 賢二 安田 | Nucleic acid recovery chip and nucleic acid recovery device |
WO2004029221A2 (en) | 2002-09-27 | 2004-04-08 | The General Hospital Corporation | Microfluidic device for cell separation and uses thereof |
US7217542B2 (en) * | 2002-10-31 | 2007-05-15 | Hewlett-Packard Development Company, L.P. | Microfluidic system for analyzing nucleic acids |
EP1590669B1 (en) * | 2003-02-05 | 2009-11-25 | The General Hospital Corporation | A microchip-based system for hiv diagnostics |
CN100516212C (en) * | 2004-05-07 | 2009-07-22 | 柯尼卡美能达医疗印刷器材株式会社 | Testing microreactor, testing device and testing method |
US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
DE102005059536B4 (en) | 2005-12-13 | 2008-08-28 | Siemens Ag | Method and biochip for studying a biological sample |
US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
EP2589668A1 (en) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
CN101544351B (en) * | 2009-05-08 | 2011-12-14 | 东南大学 | Low-dimensional nanophase material high-flexibility assembling chip and application method |
EP3049531B8 (en) | 2013-09-24 | 2019-08-14 | AIT Austrian Institute of Technology GmbH | Method for pathogen diagnosis using an electric field |
WO2015103225A1 (en) * | 2013-12-31 | 2015-07-09 | Illumina, Inc. | Addressable flow cell using patterned electrodes |
EP3112869A1 (en) * | 2015-06-30 | 2017-01-04 | IMEC vzw | Sensor device |
GB201704761D0 (en) * | 2017-01-05 | 2017-05-10 | Illumina Inc | Common line selector valve for a system |
KR102562371B1 (en) * | 2021-08-19 | 2023-08-02 | (주)엠큐빅 | Microalgae analysis device using artificial intelligence |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4661451A (en) * | 1984-02-06 | 1987-04-28 | Ortho Diagnostic Systems, Inc. | Methods for immobilizing and translocating biological cells |
US5795457A (en) * | 1990-01-30 | 1998-08-18 | British Technology Group Ltd. | Manipulation of solid, semi-solid or liquid materials |
US5605662A (en) * | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
US6071394A (en) * | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
GB9615775D0 (en) * | 1996-07-26 | 1996-09-04 | British Tech Group | Apparatus and method for characterising particles using dielectrophoresis |
-
1999
- 1999-12-22 EP EP19990968558 patent/EP1144092A4/en not_active Withdrawn
- 1999-12-22 AU AU25950/00A patent/AU763514B2/en not_active Ceased
- 1999-12-22 NZ NZ512087A patent/NZ512087A/en unknown
- 1999-12-22 CA CA002353461A patent/CA2353461A1/en not_active Abandoned
- 1999-12-22 WO PCT/US1999/031098 patent/WO2000037163A1/en not_active Application Discontinuation
- 1999-12-22 JP JP2000589268A patent/JP2002536962A/en active Pending
- 1999-12-22 KR KR1020017007947A patent/KR20010102961A/en not_active Application Discontinuation
- 1999-12-22 BR BR9916840-5A patent/BR9916840A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
NZ512087A (en) | 2003-05-30 |
WO2000037163A1 (en) | 2000-06-29 |
CA2353461A1 (en) | 2000-06-29 |
AU763514B2 (en) | 2003-07-24 |
EP1144092A1 (en) | 2001-10-17 |
KR20010102961A (en) | 2001-11-17 |
AU2595000A (en) | 2000-07-12 |
EP1144092A4 (en) | 2002-10-29 |
JP2002536962A (en) | 2002-11-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE AS 6A E 7A ANUIDADES |
|
B08H | Application fees: decision cancelled [chapter 8.8 patent gazette] |
Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1907 DE 24/07/2007 POR TER SIDO INDEVIDO. |
|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 7A E 8A ANUIDADES. |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1922 DE 06/11/2007. |