BR9809556A - "methods for the quantification of nucleic acid molecules". - Google Patents

"methods for the quantification of nucleic acid molecules".

Info

Publication number
BR9809556A
BR9809556A BR9809556-0A BR9809556A BR9809556A BR 9809556 A BR9809556 A BR 9809556A BR 9809556 A BR9809556 A BR 9809556A BR 9809556 A BR9809556 A BR 9809556A
Authority
BR
Brazil
Prior art keywords
nucleic acid
sample
molecule
quantification
target
Prior art date
Application number
BR9809556-0A
Other languages
Portuguese (pt)
Inventor
Adrew John George Simpson
Luisa Line Villa
Otavia Silva Damas D Caballero
Original Assignee
Ludwig Inst Cancer Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ludwig Inst Cancer Res filed Critical Ludwig Inst Cancer Res
Publication of BR9809556A publication Critical patent/BR9809556A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"MéTODO PARA QUANTIFICAR A EXPRESSãO DE UMA MOLéCULA DE áCIDO NUCLêICO DE INTERESSE EM UMA AMOSTRA, MéTODO PARA DETERMINAR A PROGRESSãO DE UMA CONDIçãO PATOLóGICA, E MéTODO PARA DETERMINAR UMA VARIAçãO EM UM AGENTE INFECIOSO" A presente invenção se refere a um ensaio para quantificar o número de cópias de um gene, ou o nível de sua expressão, em uma amostra biológica. O método envolve utilizar uma molécula de oligonucleotídeo, a qual irá se hibridizar com ambas a molécula de ácido nuclêico alvo e com pelo menos uma molécula de ácido nuclêico adicional na amostra. A competição entre a molécula alvo e a molécula ou moléculas adicionais resulta em um padrão de expressão o qual permite a quantificação do alvo na amostra. O método também pode ser utilizado para seguir infecções, ou o desenvolvimento de variações em um agente infeccioso."METHOD FOR QUANTIFYING THE EXPRESSION OF A NUCLEIC ACID MOLECULE OF INTEREST IN A SAMPLE, METHOD FOR DETERMINING THE PROGRESSION OF A PATHOLOGICAL CONDITION, AND METHOD FOR DETERMINING A VARIATION IN AN INFECTIOUS AGENT to refer to the present invention" to refer to this invention. number of copies of a gene, or the level of its expression, in a biological sample. The method involves using an oligonucleotide molecule, which will hybridize to both the target nucleic acid molecule and at least one additional nucleic acid molecule in the sample. The competition between the target molecule and the additional molecule or molecules results in an expression pattern which allows the quantification of the target in the sample. The method can also be used to track infections, or to develop variations in an infectious agent.

BR9809556-0A 1997-08-06 1998-08-04 "methods for the quantification of nucleic acid molecules". BR9809556A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90712997A 1997-08-06 1997-08-06
PCT/US1998/016361 WO1999007895A1 (en) 1997-08-06 1998-08-04 Methods for quantifying nucleic acid molecules

Publications (1)

Publication Number Publication Date
BR9809556A true BR9809556A (en) 2000-10-17

Family

ID=25423572

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9809556-0A BR9809556A (en) 1997-08-06 1998-08-04 "methods for the quantification of nucleic acid molecules".

Country Status (4)

Country Link
EP (1) EP0950115A4 (en)
AU (1) AU8694598A (en)
BR (1) BR9809556A (en)
WO (1) WO1999007895A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5674680A (en) * 1994-01-31 1997-10-07 The Rockefeller University Methods for the prognosis and monitoring of AIDS

Also Published As

Publication number Publication date
AU8694598A (en) 1999-03-01
WO1999007895A1 (en) 1999-02-18
EP0950115A1 (en) 1999-10-20
EP0950115A4 (en) 2001-05-30

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Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law
B11Y Definitive dismissal acc. article 33 of ipl - extension of time limit for request of examination expired