BR112022004797A2 - Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides - Google Patents
Optimized tetrahydrocannabinolic acid (thca) synthase polypeptidesInfo
- Publication number
- BR112022004797A2 BR112022004797A2 BR112022004797A BR112022004797A BR112022004797A2 BR 112022004797 A2 BR112022004797 A2 BR 112022004797A2 BR 112022004797 A BR112022004797 A BR 112022004797A BR 112022004797 A BR112022004797 A BR 112022004797A BR 112022004797 A2 BR112022004797 A2 BR 112022004797A2
- Authority
- BR
- Brazil
- Prior art keywords
- tetrahydrocannabinolic acid
- thca
- optimized
- engineered variants
- methods
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
- UCONUSSAWGCZMV-HZPDHXFCSA-N Delta(9)-tetrahydrocannabinolic acid Chemical compound C([C@H]1C(C)(C)O2)CC(C)=C[C@H]1C1=C2C=C(CCCCC)C(C(O)=O)=C1O UCONUSSAWGCZMV-HZPDHXFCSA-N 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 3
- 108030003705 Tetrahydrocannabinolic acid synthases Proteins 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 229930003827 cannabinoid Natural products 0.000 abstract 2
- 239000003557 cannabinoid Substances 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 229940065144 cannabinoids Drugs 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y121/00—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21)
- C12Y121/03—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21) with oxygen as acceptor (1.21.3)
- C12Y121/03007—Tetrahydrocannabinolic acid synthase (1.21.3.7)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
polipeptídeos otimizados de ácido tetra-hidrocanabinólico (thca) sintase. a presente divulgação fornece variantes manipuladas de um polipeptídeo de ácido tetra-hidrocanabinólico sintase (thcas) que compreende uma sequência de aminoácidos da seq id no:44 com uma ou mais substituições de aminoácido, ácidos nucleicos compreendendo sequências nucleotídicas que codificam as referidas variantes manipuladas, métodos de produção de células hospedeiras modificadas compreendendo os referidos ácidos nucleicos, células hospedeiras modificadas que expressam as referidas variantes manipuladas, métodos de produção de canabinoides ou de derivados canabinoides, e métodos de triagem de variantes manipuladas do polipeptídeo de ácido tetra-hidrocanabinólico sintase (thcas).optimized tetrahydrocannabinolic acid (thca) synthase polypeptides. the present disclosure provides engineered variants of a tetrahydrocannabinolic acid synthase (thcas) polypeptide comprising an amino acid sequence of seq id no:44 with one or more amino acid substitutions, nucleic acids comprising nucleotide sequences encoding said engineered variants, methods of producing modified host cells comprising said nucleic acids, modified host cells expressing said engineered variants, methods of producing cannabinoids or cannabinoid derivatives, and methods of screening engineered variants of tetrahydrocannabinolic acid synthase (thcas) polypeptide ).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962902300P | 2019-09-18 | 2019-09-18 | |
| PCT/US2020/051261 WO2021055597A1 (en) | 2019-09-18 | 2020-09-17 | Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112022004797A2 true BR112022004797A2 (en) | 2022-06-21 |
Family
ID=72744852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112022004797A BR112022004797A2 (en) | 2019-09-18 | 2020-09-17 | Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP4031657A1 (en) |
| JP (1) | JP2022548904A (en) |
| CN (1) | CN115038786A (en) |
| AU (1) | AU2020349513A1 (en) |
| BR (1) | BR112022004797A2 (en) |
| CA (1) | CA3152803A1 (en) |
| IL (1) | IL291328A (en) |
| MX (1) | MX2022003203A (en) |
| WO (1) | WO2021055597A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3130763A1 (en) | 2019-02-25 | 2020-09-03 | Ginkgo Bioworks, Inc. | Biosynthesis of cannabinoids and cannabinoid precursors |
| WO2023168266A2 (en) * | 2022-03-02 | 2023-09-07 | Genomatica, Inc. | Flavin-dependent oxidases having cannabinoid synthase activity |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003287028B2 (en) | 2002-10-04 | 2008-09-04 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
| CA2709107A1 (en) | 2007-12-13 | 2009-06-18 | Danisco Us Inc. | Compositions and methods for producing isoprene |
| MY153871A (en) | 2008-04-23 | 2015-03-31 | Danisco Us Inc | Isoprene synthase variants for improved microbial production of isoprene |
| CN102791848B (en) | 2008-07-02 | 2017-11-10 | 丹尼斯科美国公司 | For producing the method and composition of the isoprene without C5 hydrocarbon in the case where removing coupling condition and/or safe operating range |
| JP7198555B2 (en) * | 2017-04-27 | 2023-01-04 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Microorganisms and methods for producing cannabinoids and cannabinoid derivatives |
| EP3652327A4 (en) * | 2017-07-12 | 2021-04-21 | Biomedican, Inc. | Production of cannabinoids in yeast |
| CA3123182A1 (en) * | 2017-12-14 | 2019-06-20 | Medicinal Genomics Corporation | Methods and kits for classifying cannabinoid production in cannabis plants |
| WO2020069214A2 (en) * | 2018-09-26 | 2020-04-02 | Demetrix, Inc. | Optimized expression systems for producing cannabinoid synthase polypeptides, cannabinoids, and cannabinoid derivatives |
-
2020
- 2020-09-17 WO PCT/US2020/051261 patent/WO2021055597A1/en unknown
- 2020-09-17 JP JP2022517341A patent/JP2022548904A/en active Pending
- 2020-09-17 CA CA3152803A patent/CA3152803A1/en active Pending
- 2020-09-17 BR BR112022004797A patent/BR112022004797A2/en not_active Application Discontinuation
- 2020-09-17 CN CN202080078001.1A patent/CN115038786A/en active Pending
- 2020-09-17 EP EP20786122.0A patent/EP4031657A1/en not_active Withdrawn
- 2020-09-17 AU AU2020349513A patent/AU2020349513A1/en active Pending
- 2020-09-17 MX MX2022003203A patent/MX2022003203A/en unknown
-
2022
- 2022-03-13 IL IL291328A patent/IL291328A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA3152803A1 (en) | 2021-03-25 |
| IL291328A (en) | 2022-05-01 |
| EP4031657A1 (en) | 2022-07-27 |
| CN115038786A (en) | 2022-09-09 |
| JP2022548904A (en) | 2022-11-22 |
| AU2020349513A1 (en) | 2022-05-05 |
| MX2022003203A (en) | 2022-06-08 |
| WO2021055597A1 (en) | 2021-03-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |