BR0212098A - Método de isolamento de massa celular interna para o estabelecimento de linhas de células tronco embriÈnicas humanas (hesc) - Google Patents

Método de isolamento de massa celular interna para o estabelecimento de linhas de células tronco embriÈnicas humanas (hesc)

Info

Publication number
BR0212098A
BR0212098A BR0212098-4A BR0212098A BR0212098A BR 0212098 A BR0212098 A BR 0212098A BR 0212098 A BR0212098 A BR 0212098A BR 0212098 A BR0212098 A BR 0212098A
Authority
BR
Brazil
Prior art keywords
embryonic stem
human embryonic
cell mass
hesc
establishment
Prior art date
Application number
BR0212098-4A
Other languages
English (en)
Inventor
Firuza Rajesh Parikh
Satish Mahadeorao Totey
Shailaja Anupam Saxena
Original Assignee
Reliance Life Sciences Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reliance Life Sciences Pvt Ltd filed Critical Reliance Life Sciences Pvt Ltd
Publication of BR0212098A publication Critical patent/BR0212098A/pt

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/42Gynaecological or obstetrical instruments or methods
    • A61B17/425Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
    • A61B17/435Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B18/00Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
    • A61B18/18Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by applying electromagnetic radiation, e.g. microwaves
    • A61B18/20Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by applying electromagnetic radiation, e.g. microwaves using laser
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Surgery (AREA)
  • Reproductive Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Medical Informatics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Otolaryngology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Transplantation (AREA)
  • Optics & Photonics (AREA)
  • Electromagnetism (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

"MéTODO DE ISOLAMENTO DE MASSA CELULAR INTERNA PARA O ESTABELECIMENTO DE LINHAS DE CéLULAS TRONCO EMBRIÈNICAS HUMANAS (HESC)". Um método de isolamento de massa celular interna (ICM) que compreende as etapas de imobilização de um embrião em estágio de blastocisto tendo uma zona pelúcida, trofectoderma, e uma massa celular interna, criando um orifício no embrião em estágio de blastocisto por ablação a laser, e remoção da massa celular interna do embrião em estágio de blastocisto através do orifício. O orifício é através da zona pelúcida e trofectoderma. O orifício é atingido usando um laser de diodo de não contato. A massa celular interna removida do embrião em estágio de blastocisto é usada para estabelecer linhas de células tronco embriónicas humanas.
BR0212098-4A 2001-08-23 2002-08-20 Método de isolamento de massa celular interna para o estabelecimento de linhas de células tronco embriÈnicas humanas (hesc) BR0212098A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31432301P 2001-08-23 2001-08-23
PCT/IN2002/000168 WO2003018783A1 (en) 2001-08-23 2002-08-20 ISOLATION OF INNER CELL MASS FOR THE ESTABLISHMENT OF HUMAN EMBRYONIC STEM CELL (hESC) LINES

Publications (1)

Publication Number Publication Date
BR0212098A true BR0212098A (pt) 2004-08-03

Family

ID=23219502

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0212098-4A BR0212098A (pt) 2001-08-23 2002-08-20 Método de isolamento de massa celular interna para o estabelecimento de linhas de células tronco embriÈnicas humanas (hesc)

Country Status (13)

Country Link
US (2) US7294508B2 (pt)
EP (1) EP1421181A1 (pt)
JP (3) JP2005514004A (pt)
KR (1) KR20040030161A (pt)
CN (1) CN1545549A (pt)
AU (1) AU2002334378B2 (pt)
BR (1) BR0212098A (pt)
CA (1) CA2458362A1 (pt)
IL (2) IL160351A0 (pt)
RU (1) RU2291703C2 (pt)
SG (1) SG172471A1 (pt)
WO (1) WO2003018783A1 (pt)
ZA (1) ZA200304132B (pt)

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US20050272149A1 (en) 2004-06-02 2005-12-08 Life & Brain Gmbh Therapeutic delivery of adenosine into a tissue
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EP2245141B1 (en) 2008-01-18 2018-03-14 Regents of the University of Minnesota Stem cell aggregates and methods for making and using
WO2010114193A1 (ko) * 2009-03-29 2010-10-07 Kim Chang-Hyun 내부 세포 덩어리의 분리 방법 및 이를 이용한 배아 줄기 세포주의 제조 방법
JP6243119B2 (ja) 2009-07-21 2017-12-06 エイビーティー ホールディング カンパニー 白血球溢出を低下させる幹細胞の使用
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CN103237886B (zh) 2010-08-24 2018-10-30 明尼苏达大学董事会 细胞集合体的非静态悬浮培养
WO2012048298A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions
GB2502704B (en) 2010-11-15 2019-12-04 Accelerated Biosciences Corp Generation of neural stem cells from human trophoblast stem cells
US8748178B2 (en) 2010-11-23 2014-06-10 The New York Stem Cell Foundation Method for producing pluripotent stem cells
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Also Published As

Publication number Publication date
US20030104616A1 (en) 2003-06-05
JP2005514004A (ja) 2005-05-19
IL160351A (en) 2009-11-18
CN1545549A (zh) 2004-11-10
IL160351A0 (en) 2004-07-25
JP2008099706A (ja) 2008-05-01
US7294508B2 (en) 2007-11-13
US20080064099A1 (en) 2008-03-13
WO2003018783A1 (en) 2003-03-06
US20070048864A2 (en) 2007-03-01
KR20040030161A (ko) 2004-04-08
RU2004108209A (ru) 2005-04-20
AU2002334378B2 (en) 2008-02-28
CA2458362A1 (en) 2003-03-06
EP1421181A1 (en) 2004-05-26
SG172471A1 (en) 2011-07-28
RU2291703C2 (ru) 2007-01-20
ZA200304132B (en) 2004-07-19
JP2008194055A (ja) 2008-08-28

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B15K Others concerning applications: alteration of classification

Ipc: A61B 17/435 (2006.01), A61B 18/20 (2006.01), C12N

B11T Dismissal of application maintained [chapter 11.20 patent gazette]