AU770885B2 - Genetic suppressor elements against human immunodeficiency virus - Google Patents

Description

WO 00/37635 PCT/US99/30187 GENETIC SUPPRESSOR ELEMENTS AGAINST HUMAN IMMUNODEFICIENCY VIRUS 1. INTRODUCTION The present invention relates to genetic elements that suppress the activities of the human immunodeficiency virus (HIV). In particular, the invention relates to polynucleotides isolated from the HIV-1 genome, methods for isolating, identifying and designing such polynucleotides, and methods for using them for the protection of human cells against HIV infection and/or replication.

2. BACKGROUND OF THE INVENTION 2.1. THE HUMAN IMMUNODEFICIENCY VIRUS The primary cause of acquired immunodeficiency syndrome (AIDS) has been shown to be HIV (Barre-Sinoussi et al., 1983, Science 220:868-870; Gallo et al., 1984, Science 224:500-503).

HIV causes immunodeficiency in an individual by infecting important cell types of the immune system, which results in their depletion. This, in turn, leads to opportunistic infections, neurological dysfunctions, neoplastic growth, and death.

HIV is a member of the lentivirus family of retroviruses (Teich et al., 1984, RNA Tumor Viruses, Weiss et al., eds., CSH-Press, pp. 949-956). Retroviruses are small enveloped viruses that contain a diploid, single-stranded RNA genome, and replicate via a DNA intermediate produced by a virallyencoded reverse transcriptase, an RNA-dependent DNA polymerase (Varmus, 1988, Science 240:1427-1439). There are at least two distinct subtypes of HIV: HIV-1 (Barre-Sinoussi et al., 1983, Science 220:868-870; Gallo et al., 1984, Science 224:500-503) and HIV-2 (Clavel et al., 1986, Science 233:343-346; Guyader et al., 1987, Nature 326:662-669). Genetic heterogeneity exists within each of these HIV subtypes.

CD4 T cells are the major targets of HIV infection because the CD4 cell surface protein acts as a cellular receptor for HIV attachment (Dalgleish et al., 1984, Nature WO 00/37635 PCT/US99/30187 312:763-767; Klatzmann et al., 1984, Nature 312:767-768; Maddon et al., 1986, Cell 47:333-348). Viral entry into cells is dependent upon viral protein gpl20 binding to the cellular CD4 receptor molecule (McDougal et al., 1986, Science 231:382- 385; Maddon et al., 1986, Cell 47:333-348), and a chemokine co-receptor such as CXCR4 or CCR5 (Moore, 1997, Science 276:51-52).

2.2. HIV TREATMENT HIV infection is pandemic and HIV-associated diseases have become a world-wide health problem. Despite considerable efforts in the design of anti-HIV modalities, there is, thus far, no successful prophylactic or therapeutic regimen against AIDS. However, several stages of the HIV life cycle have been considered as potential targets for therapeutic intervention (Mitsuya et al., 1991, FASEB J. 5:2369-2381). For example, virally-encoded reverse transcriptase has been a major focus of drug development. A number of reverse-transcriptasetargeted drugs, including 2',3'-dideoxynucleotide analogs such as AZT, ddl, ddC, and ddT have been shown to be active against HIV (Mitsuya et al., 1990, Science 249:1533-1544). While beneficial, these nucleotide analogs are not curative, probably due to the rapid appearance of drug resistant HIV mutants (Lander et al., 1989, Science 243:1731-1734). In addition, the drugs often exhibit toxic side effects, such as bone marrow suppression, vomiting, and liver abnormalities.

Another stage of the HIV life cycle that has been targeted is viral entry into the cells, the earliest stage of HIV infection. This approach has primarily utilized recombinant soluble CD4 protein to inhibit infection of CD4 4

T

cells by some HIV-1 strains (Smith et al., 1987, Science 238:1704-1707). Certain primary HIV-1 isolates, however, are relatively less sensitive to inhibition by recombinant CD4 (Daar et al., 1990, Proc. Natl. Acad. Sci. USA 87:6574-6579).

To date, recombinant soluble CD4 clinical trials have produced inconclusive results (Schooley et al., 1990, Ann. Int. Med.

112:247-253; Kahn et al., 1990, Ann. Int. Med. 112:254-261; WO 00/37635 PCT/US99/30187 Yarchoan et al., 1989, Proc. Vth Int. Conf. on AIDS, p. 564, MCP 137).

Additionally, the later stages of HIV replication which involve crucial virus-specific secondary processing of certain viral proteins and enzymes have also been targeted for anti- HIV drug development. Late stage processing is dependent on the activity of a virally-encoded protease, and drugs including nelfinavir, saquinavir, ritonavir, and indinavir have been developed to inhibit this protease (Pettit et al., 1993, Persp. Druc. Discov. Design 1:69-83). With this class of drugs, the emergence of drug resistant HIV mutants is also a problem; resistance to one inhibitor often confers cross resistance to other protease inhibitors (Condra et al., 1995, Nature 374:569-571). These drugs often exhibit toxic side effects such as nausea, altered taste, circumoral parethesias, lipodystrophy and nephrolithiasis. Furthermore, these drugs also interact with many drugs commonly used to treat HIV infection (Flexner, 1998, N. Engl. J. Med. 338:1281-1292) Antiviral therapy of HIV using different combinations of nucleoside analogs and protease inhibitors have recently been shown to be more effective than the use of a single drug alone (Torres et al., 1997, Infec. Med. 14:142-160). However, despite the ability to achieve significant decreases in viral burden, there is no evidence to date that combinations of available drugs will afford a curative treatment for AIDS.

Other approaches for developing treatment for AIDS include the delivery of exogenous genes into infected cells.

One such gene therapy approach involves the use of genetically-engineered viral vectors to introduce toxic gene products to kill HIV-infected cells. For instance, replication defective vectors have been designed to introduce cell growth inhibitory genes into host cells (WO 90/12087, October 18, 1980). One strategy attempted by several groups involves the delivery of the herpes simplex virus type 1 thymidine kinase (tk) toxin gene. The tk gene product is toxic to mammalian cells only in the presence of nucleoside WO 00/37635 PCTIUS99/30187 analogs, such as ganciclovir (Ventakash et al., 1990, Proc.

Natl. Acad. Sci. USA 87: 8746-8750; Brady et al., 1994, Proc.

Natl. Acad. Sci. USA 91: 365-369; WO 90/07936, July 26,1990) Diphtheria toxin gene has also been used, and the gene was placed under the control of cis-acting HIV regulatory sequences Patent 5,306,631, issued April 26, 1994) Others have utilized replication incompetent mutants of HIV which have the potential to express an inhibitory gene product in the presence of HIV tat (WO 94/16060, July 21, 1994).

Another form of gene therapy is designed to protect virally-infected cells from cytolysis by specifically disrupting viral replication. Efforts to identify appropriate protective genes have, in large part, been based on an understanding of the molecular biology of HIV replication. A few examples of this approach are as follows.

The HIV-1 Rev gene encodes a protein that is necessary for the expression of full length HIV-1 transcripts in infected cells and the production of HIV-1 virions.

Transfection with one Rev mutant known as RevM10 has been shown to protect the cells against HIV infection (Malim et al., 1992, J.Exp. Med. 176:1197; Bevec et al., 1992, Proc.

Natl. Acad. Sci. USA 89:9870-74). Typically, the transfectants are resistant to HIV-1 infection for about 2 weeks from the time of inoculation before resistant variants appear (Woffendin et al., 1994, Proc. Natl. Acad. Sci. USA 91: 11581-85).

In addition, Rev function can be interfered with by producing an excess of the binding site of the Rev protein, termed Rev Response Element (RRE), which prevents the binding of Rev to RRE of viral transcripts. A "decoy" which consisted of a chimeric RNA composed of an RRE and a tRNA prevented infection of cultured cells for a period of greater than about 40 days (Lee et al., 1994, J.Virology 68:8254-64).

Alternatively, fusion proteins capable of binding to viral env proteins have been made to prevent the production of HIV-1 virions. Examples include a fusion protein composed of WO 00/37635 PCT/US99/30187 CD4 and a lysosomal targeting protein procathepsin D, and an anti-env Fv which is secreted into the endoplasmic reticulum (Lin et al., WO 93/06216; Marasco et al., 1993, Proc. Natl.

Acad. Sci. USA 90:7889-93).

Antisense polynucleotides have also been designed to complex with and sequester the HIV-1 transcripts (Holmes et al., WO 93/11230; Lipps et al., WO 94/10302; Kretschmer et al., EP 594,881; and Chatterjee et al., 1992, Science 258:1485). Furthermore, an enzymatically active RNA, termed ribozyme, has been used to cleave viral transcripts. The ribozyme approach to forming an HIV-1 resistant hematopoietic cell line has been reported (Ojwang et al., 1992, Proc. Natl.

Acad. Sci. USA 89:10802-06; Yamada et al., 1994, Gene Therapy 1:38-45; Ho et al., WO 94/26877; and Cech and Sullenger, WO 95/13379).

Roninson et al. described a method for isolating genetic fragments from the HIV-1 genome capable of protecting a cell from HIV-1 infection Patent No. 5,217,889 and WO 92/07071). The method involves the preparation of an expression library known as a Random Fragment Expression (RFE) library that contains random sequence fragments of the HIV-1 genome. Gene fragments referred to as HIV-1 Genetic Suppressor Element (HIV-1 GSE) are then selected from the RFE library following an extensive selection procedure. The selection step involves transfection of the RFE library into a cell line to which HIV-1 infection is normally cytotoxic.

However, the low sensitivity of this selection step greatly limits the practical use of the procedure. Moreover, no specific GSE sequences were reported using this method that were capable of suppressing HIV-1 infection.

3. SUMMARY OF THE INVENTION The present invention relates to specific HIV-derived polynucleotides herein referred to as GSEs that suppress HIV infection and/or replication in human cells, methods for isolating and identifying such polynucleotides, methods for WO 00/37635 PCT/US99/30187 designing such polynucleotides, and methods for using them in the prevention or treatment of HIV infection.

The invention is based, in part, on the Applicants' discovery that nucleotide fragments can be isolated from the HIV-1 genome, based on their ability to suppress the activation of latent HIV-1 in a CD4' cell line or on their ability to prevent cells from productive infection by HIV-1.

In this connection, any cellular or viral marker associated with HIV replication can be used to monitor the activation of latent HIV or productive infection of cells. A number of novel HIV-1 GSE polynucleotides are selected on the basis of their ability to sustain CD4 expression by the induced cells, and several of such sequences are further shown to protect uninfected T cells from productive infection by HIV-1. The GSEs may function in the form of an RNA product or protein product, both of which are within the scope of the invention.

Another exemplary marker is the intracellular p24.

Replication of HIV in susceptible cells is associated with accumulation of intracellular p24, concomitant with down modulation of surface CD4. The expression of GSEs capable of interfering with productive infection should result in enrichment of protected cells displaying the CD4 p24phenotype. Such cells can be separated by FACS from infected population.

This invention is also based upon the discovery that isolated GSEs cluster around narrowly defined regions of the HIV-1 genome, particularly, the surprising discovery that inhibitory or protective clusters of GSEs were also isolated from previously untargeted regions (RT and nef). Preferred embodiments of the invention provide methods for designing GSEs based upon the consensus sequences of such GSE clusters.

A wide range of uses are encompassed by the invention, including but not limited to, AIDS treatment and prevention by transferring GSE into HIV-l-susceptible cell types. For example, GSE may be transferred into T cells or hematopoietic progenitor cells in vitro followed by their engraftment in an autologous, histocompatible or even histoincompatibile 7 recipient. Alternatively, any cells susceptible to HIV infection may be directly transduced or transfected with GSE in vivo.

Accordihgly, in a first embodiment of the present invention there is provided an isolated polynucleotide, consisting of a nucleotide sequence recited in any one of SEQ ID NOS: 1 to 25, or comprising a fragment of a gene comprising a nucleotide sequence recited in any one of SEQ ID NOS: 2 to 6, 8 to 11, 14, 15, or 17 to 25, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a second embodiment of the present invention there is provided an 0o isolated polynucleotide consisting of a nucleotide sequence recited in any one of SEQ ID NOS: 1 to According to a third embodiment of the present invention there is provided an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence recited in any one of SEQ ID NOS: 2 to 6, 8 to 11, 14, 15, or 17 to 25, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a fourth embodiment of the present invention there is provided an isolated polynucleotide consisting of a nucleotide sequence that is fully complementary to a nucleotide sequence of any one of SEQ ID NOS: 1 to o. 20 According to a fifth embodiment of the present invention there is provided an isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO:1, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a sixth embodiment of the present invention there is provided an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:2, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a seventh embodiment of the present invention there is provided an 30 isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:3, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to an eighth embodiment of the present invention there is provided an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide [R:\LIBVV]04443.doc:aak sequence of SEQ ID NO:4, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a ninth embodiment of the present invention there is provided an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:5, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a tenth embodiment of the present invention there is provided an 0o isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:6, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to an eleventh embodiment of the present invention there is provided an isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO:7, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a twelfth embodiment of the present invention there is provided an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide 20 sequence of SEQ ID NO:8, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a thirteenth embodiment of the present invention there is provided an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:9, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

S. According to a fourteenth embodiment of the present invention there is provided an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide 30 sequence of SEQ ID NO:10, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a fifteenth embodiment of the present invention there is provided an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:11, wherein said nucleotide sequence is operably linked to a [R:\LIBVV]04443.doc:aak regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a sixteenth embodiment of the present invention there is provided an isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO:12, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a seventeenth embodiment of the present invention there is provided an isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO: 13, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein to expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to an eighteenth embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:14, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a nineteenth embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:15, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses 20 infection by HIV-1.

According to a twentieth embodiment of the present invention there is an isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO:16, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a twenty-first embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:17, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses •infection by HIV-1.

According to a twenty-second embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:18, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

[R:\LIBVV]04443.doc:aak 7c According to a twenty-third embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:19, wherein said iucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a twenty-fourth embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:20, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell to suppresses infection by HIV-1.

According to a twenty-fifth embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:21, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a twenty-sixth embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:22, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a twenty-seventh embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:23, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a twenty-eighth embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:24, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell S 30 suppresses infection by HIV-1.

•According to a twenty-ninth embodiment of the present invention there is an isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:25, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

[R:\LIBVV]04443.doc:aak 7d According to a thirtieth embodiment of the present invention there is provided a recombinant vector containing the isolated polynucleotide of any one of the first to the twenty-ninth embodiments.

According to a thirty-first embodiment of the present invention there is provided a genetically engineered host cell that contains the vector of the thirtieth embodiment.

According to a thirty-second embodiment of the present invention there is provided a method of inhibiting HIV infection, the method comprising the step of contacting a cell with the polynucleotide of any one of the first to the twenty-ninth embodiments, thereby inhibiting HIV replication in said cell.

According to a thirty-third embodiment of the present invention there is provided a method of treating HIV infection in a subject, the method comprising the step of administering to said subject a therapeutically effective amount of the polynucleotide of any one of the first to the twenty-ninth embodiments.

According to a thirty-fourth embodiment of the present invention there is provided Is an isolated polynucleotide, consisting of a first nucleotide sequence that hybridizes under highly stringent conditions to a second nucleotide sequence that is complementary to any one of SEQ ID NOS: 2 to 5, 8, 9, 11, 14, 15 or 17 to 25, wherein said first nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

According to a thirty-fifth embodiment of the present invention there is provided a recombinant vector containing the isolated polynucleotide of the thirty-fourth embodiment.

According to a thirty-sixth embodiment of the present invention there is provided a genetically engineered host cell that contains of the vector of the thirtieth-fifth embodiment.

According to a thirty-seventh embodiment of the present invention there is provided *0a method of inhibiting HIV infection, the method comprising the step of contacting a cell with the polynucleotide of the thirty-fourth embodiment, thereby inhibiting HIV eeee °replication in said cell.

30 According to a thirty-eighth embodiment of the present invention there is provided a method of treating HIV infection in a subject, the method comprising the step of ".•."administering to said subject a therapeutically effective amount of the polynucleotide of the thirty-fourth embodiment.

[R:\LIBVV]04443.doc:aak 4. Brief Description of the Drawings Figure 1. Percentage of CD4 OM10.1 cells diminishes after TNF-a induction, TNF-induced cells, 0 uninduced cells, Figure 2. HIV p24 level increases in OM10.1 cells after TNF-a induction; s TNF-induced cells, uninduced cells, E Figure 3. Location of isolated HIV GSE on the HIV-BRU genome. Arrows indicate antisense orientation elements, while shaded boxes indicate sense orientation elements. Scale of the HIV-1 genome is in kilobases.

Figure 4. CD4 retention levels of OM10.1 cells containing GSEs after TNF-a induction.

Figure 5A&B. Time course of viral infection of CEM-ss cells containing GSEs.

Results are presented as the percentage of p24 positive cells at specified days post infection. All results are of a representative experiment. Figure 5A: CEM-ss cells containing IGX-117 (vpr/tat sense, SEQ ID NO:9), IGX-201 (RRE antisense, SEQ ID and controls were infected with a TCID 50 of 500 of HIV- 1SF2. Figure 5B: CEM-ss cells containing IGX-104 (RT sense, SEQ ID NO:2) and controls were infected with a TCID5o of 3000 20 of HIV-1IB.

5. Detailed Description of the Invention :The present invention relates to specific HIV-derived polynucleotides identified by an improvement of the method disclosed by Roninson et al. in United States Patent No.

5,217,889. More specifically, the improvement of the method includes the use of a cell line containing a latent and oe* *oo o [R:\LIBVV]04443.doc:aak WO 00/37635 PCTUS99/30187 inducible HIV-I provirus such as OM10.1. In addition, the improvement also encompasses the use of a marker associated with HIV infection such as CD4 to select for polynucleotides from an HIV-l RFE library that effectively suppress HIV-I infection. The GSEs selected by this procedure are also capable to protect uninfected cells from HIV infection.

In another aspect of the invention, GSEs are isolated based upon their ability to prevent cells from productive infection by HIV-1.

Generally, the method of the invention includes the steps of: 1) fragmenting the HIV-I genome into 100-700 base pair (bp) fragments; 2) inserting the fragments into expression vectors such that the fragments are transcribed and translated to form an expression library; 3) transferring the expression library into a population of cells containing an inducible latent HIV-I provirus or cells susceptible to HIV infection; 4) selecting a subpopulation of cells which contain a subset of the expression library enriched for HIV-1 GSEs by monitoring the expression of a cellular or viral marker associated with HIV infection; and 5) recovering the GSEs from the selected cell population. The method further includes repetition of the aforementioned steps with a secondary or tertiary library so that many rounds of successive selection can be performed. The selection of GSEs can be performed by monitoring the continued expression of a cellular marker such as CD4 or the decreased expression of a viral marker such as p24 or gpl20 using an antibody.

The invention is discussed in more detail in the subsections below, solely for purposes of description and not by way of limitation. For clarity of discussion, the specific procedures and methods described herein are exemplified using OMI0.1 cells, CEM-ss cells, tumor necrosis factor-alpha (TNFan anti-CD4 antibody, and an anti-p24 antibody, but they are merely illustrative for the practice of the invention.

Analogous procedures and techniques are equally applicable to isolating GSE from different subtypes of HIV, utilizing any cell line containing an inducible latent provirus or any cell WO 00/37635 PCT/US99/30187 line or freshly isolated cell population susceptible to HIV infection, and any marker associated with HIV infection that can be easily assayed.

5.1. PREPARATION AND TRANSFECTION OF AN HIV-1 GSE LIBRARY An HIV RFE library can be constructed from the DNA of a plasmid or multiple plasmids that contain an HIV provirus insert. HIV proviral DNA is first treated with enzymes to produce randomly cleaved fragments. This can be conveniently performed by DNase I cleavage in the presence of Mn" (Roninson et al., Patent No. 5,217,889, column 5, lines 5-20).

Thereafter, the randomly cleaved genomic DNA are size fractionated by gel electrophoresis. Fragments of between 100 and 700 bp are the preferred lengths for constructing RFE libraries. Single strand breaks of the size-selected fragments are repaired, by Klenow or T4 polymerase, and ligated with 5' and 3' adaptors.

The 5' and 3' adaptors are selected to have non-cohesive restriction sites so that each fragment can be inserted into an expression vector in an oriented fashion. Further, the adaptor contains at least one start (ATG) codon to allow the translation of the fragments which contain an open reading frame in the correct phase.

After ligation with the adaptors the fragments are inserted into appropriate expression vectors. Any expression vector that results in efficient expression of the fragments in host cells can be used. In a preferred embodiment viral based vectors such as the retroviral vectors LXSN and a modified LSXN vector, LXSNgfr, are exemplified (Miller and Rosman, 1989, BioTechniques 7:980). Alternatively, adenoassociated virus vectors may also be used for this purpose.

When viral-based vectors are used, the ligated vectors are first transfected into a packaging cell line to produce viral particles. For retroviral vectors, any amphotropic packaging line such as PA317 (Miller and Buttimore, 1986, Mol.

Cell. Biol. 6:2895-2902; ATCC CRL #9078) or BING may be used WO 00/37635 PCT/US99/30187 to efficiently produce virus. In a preferred embodiment of the invention, the viral vector also contains a selectable gene, such as the neo r gene or a truncated nerve growth factor receptor (NGFR) gene, which allow isolation of the cells that contain the vector.

The number of independent clones present in each GSE expression library may vary. In a preferred embodiment, libraries of about 5 x 104 to 106 independent clones may be used.

5.2. SELECTION OF GSE IN HIV-INFECTED CELLS In a specific embodiment by way of example, OM10.1 cells are used to select for GSEs, and they are maintained in conventional tissue culture as described in Butera (U.S.

Patent No. 5,256,534). The purpose of using OM10.1 cells for the selection of HIV-1 GSEs is that they contain a latent HIV- 1 provirus which is inducible by TNF-a. Other cell lines may be similarly engineered with an inducible HIV provirus.

Examples of cell lines that are infected with latent HIV include, but are not limited to, Ul, U33, 8E5, ACH-2, LL58, THP/HIV and UHC4 (Bednarik and Folks, 1992, AIDS 6:3-16). A variety of agents have been shown to be capable of inducing latent HIV-infected cells, and these include TNF-a, TNF-3, interleukins-1, -4 and granulocyte-macrophage colony stimulating factors, macrophage-colony stimulating factors, interferon-y, transforming growth factor-1, PMA, retinoic acid and vitamin D3 (Poli and Fauci, 1992, AIDS Res.

Human Retroviruses 9:191-197).

The HIV-infected cells may be transduced with the HIV-1 RFE library by any technique well known in the art that is appropriate to the vector system employed. In one embodiment of the invention, the viral vector also contains a selectable marker in addition to a random fragment of the HIV-1 genome.

A suitable marker is the neor gene, which permits selection by the drug G-418. In alternative embodiments, the multiplicity of infection of the virions of the library is adjusted so that WO 00/37635 PCT/US99/30187 pre-selection for cells that are transduced by the vector is not needed.

In the case of OM10.1 cells, the transduced population is treated with 10 U/ml TNF-a for a period of 24-72 hours and preferably about 24 hours according to the method of Butera.

The activation of the latent HIV-1 provirus in OM10.1 can be detected by the suppression of the cell surface CD4. It is believed that viral protein gpl20 binds to CD4 in the cytoplasm, which prevents subsequent expression of CD4 on the cell surface. Clones that are resistant HIV replication continue to express cell surface CD4. Such clones can be selected by cell sorting using any conventional antibody staining technique for CD4 and a fluorescence activated cell sorter (FACS).

After selection for continued CD4 expression, the OM10.1 cells harboring putative GSE and sorted after TNF-a induction are used to purify genomic DNA and the inserts amplified by the polymerase chain reaction (PCR). Optionally, the selected OM10.1 cells can be re-cultured under the selection conditions for the marker gene, in G-418, to ensure that the cells have retained the GSE derived from the HIV-1 RFE library.

The fraction of CD4' cells that have been transduced with an HIV-1 RFE library can be compared with cells transduced with an expression library consisting of the vector only. An increased relative difference between the HIV-1 RFE library and the control library can be found with each additional round of TNF-a induction. Thus, in the preferred embodiment of the invention there are at least two cycles of induction, selection and reculturing before the HIV-1 GSE are recovered from the cells for further characterization.

5.3. RECOVERY GSE FROM THE SELECTED CELLS After selection, specific GSE sequences can be recovered from cells that continue to express CD4 following induction of the latent HIV provirus by TNF-a. The HIV-l-associated GSEs in this population are recovered by amplification in PCR using the primers according to the sequence of the linkers.

WO 00/37635 PCT/US99/30187 The recovered GSEs can be introduced into an expression vector as discussed in Section 5.1, supra. The resultant HIV- 1 GSE expression library is known as a secondary library. The secondary library may utilize the same or a different vector from that used for the construction of the primary library.

The secondary library may be transduced into another cell population and the resultant population selected, recultured and processed as described herein.

Additionally, each individually recovered element can be inserted into cloning vectors for determining its specific nucleotide sequence, its orientation and the portion of HIV genome from which it is derived. Concurrently, the isolated GSEs can be analyzed to determine their minimal core sequences and tested for their ability to protect previously uninfected cells from HIV infection.

In addition to the sequences depicted in SEQ ID NOS: 1nucleotide sequences capable of hybridizing to these sequences or their complementary sequences under highly or moderately stringent hybridization conditions are well within the scope of the invention. Highly stringent hybridization conditions may be defined as hybridization to filter-bound DNA in 0.5 M NaHPO4, 7% sodium dodecyl sulfate (SDS), imM EDTA at 0 C, followed by washing in 0.1 x SSC/0.1% SDS at 68 0

C

(Ausubel F.M. et al., eds, 1989, Current Protocols in Molecular Biology, Vol. I, Green Publishing Associates, Inc., and John Wiley Sons, Inc., New York at p. 2.10.3). Less highly stringent conditions, such as moderately stringent conditions, may be defined as hybridizations carried out as described above, followed by washing in 0.2x SSC/0.1% SDS at 42 0 C (Ausubel et al., 1989, supra).

5.4. DETERMINATION OF CORE SEQUENCES OF GSE The present invention also includes methods for determining the core sequence of each GSE. This may be done by comparing overlapping sequences of independently derived GSEs. Figure 3 depicts a number of clusters of independently derived GSEs. A surprising discovery of the invention is that WO 00/37635 PCT/US99/30187 isolated GSEs are clustered around narrowly defined regions of the HIV-1 genome. In addition, clusters of GSEs from previously untargeted regions reverse transcriptase and nef) were also isolated. In Figure 3, a number of GSEs selected on the basis of their ability to prevent productive infection are clustered in the region (RT) with coordinates 2426-2511 of the HIV-1 isolate BRU (GenBank accession number K02013). Such GSEs also showed inhibitory effects upon in virally-infected cells (Figure One of skill in the art would appreciate that sequences selected from this region are expected to be active in preventing productive infection and in inhibiting induction of latently infected cells.

Similarly, a number of GSEs are clustered around the region 8195-8599 of the HIV-1 genome in both sense and antisense orientation. It would be apparent to those of skill in the art that nucleotides containing sequences selected from this region are likely to be active in preventing productive infection and/or inhibiting virus induction in latently infected cells.

Depending upon the mechanisms of action of specific GSEs, the length of GSE sequences may affect their ability of inhibiting HIV-1 infection. The sequences selected from the regions of the viral genome depicted in Figure 3 are preferably at least 25 nucleotides, more preferably at least nucleotides, and most preferably at least 100 nucleotides in length.

Alternatively, GSEs may be altered by additions, substitutions or deletions and assayed for retention of HIVsuppressive function. Alterations in the GSE sequences may be generated using a variety of chemical and enzymatic methods which are well known to those skilled in the art. For example, oligonucleotide-directed mutagenesis may be employed to alter the GSE sequence in a defined way and/or to introduce restriction sites in specific regions within the sequence.

Additionally, deletion mutants may be generated using DNA nucleases such as Bal 31 or Exo III and S1 nuclease.

Progressively larger deletions in the GSE sequences may be WO 00/37635 PCT/US99/30187 generated by incubating the DNA with nucleases for increased periods of time (See Ausubel, et al., 1989 Current Protocols for Molecular Biology, for a review of mutagenesis techniques).

The altered sequences may be evaluated for their ability to suppress expression of HIV proteins such as p24 in appropriate host cells. It is well within the scope of the present invention that any altered GSE sequences that retain their ability to suppress HIV infection may be incorporated into recombinant expression vectors for further use.

PROTECTION OF UNINFECTED CELLS BY GSE AGAINST HIV-1 INFECTION In order to confirm that the selected GSEs can protect uninfected cells from HIV-1 infection, the GSEs may be transferred into HIV susceptible host cells, followed by HIV infection. Protection experiments can be performed in any cell type that takes up the potential HIV-1 GSEs and which is otherwise susceptible to HIV infection. In a preferred embodiment by way of example, the CEM-ss cell line is used (Foley et al., 1965, Cancer 18:522-29). The use of CEM-ss cells as targets for quantitative infectivity of HIV-1 has been described by Nara Fischinger (1988, Nature 322:469-70) Other cell lines that are susceptible to HIV infection include, but are not limited to, HUT-78, H9, Jurkat E6-1, A3.01, U-937, AA-2, HeLa CD4' and C8166.

The test of the potential HIV-1 GSEs can be performed using the same expression vector system as that employed in the RFE library transduction of cells during initial selection steps. In other embodiments, the vector system can be modified to achieve higher levels of expression, linkers can be employed to introduce a leader sequence that increases the translational efficiency of the message. One such sequence is disclosed by Kozak, 1994, Biochemie 76:815-821.

Another way of testing the effectiveness of a GSE against HIV is to determine how rapidly HIV-1 variants develop that can negate the effects of the potential HIV-1 GSE. Such a WO 00/37635 PCT/US99/30187 test includes infection of a culture of susceptible cells such as CEM-ss cells at a low multiplicity of infection and repeatedly assaying the culture to determine whether and how quickly HIV-1 infection becomes widespread. The range of useful multiplicities of infection is between about 100 to 1000 tissue culture infectious units (TCID 50 per 106 CEM-ss cells. The TCID, 5 is determined by an endpoint method and is important for determining the input multiplicity of infection (moi).

A parameter that correlates with the development in the test culture of HIV-1 strains that are resistant to the effects of the potential HIV-1 GSE is the fraction of cells that are infected in the culture. This fraction can be determined by any means. Immunofluorescent staining with an antibody specific for the HIV-1 p24 antigen of fixed permeabilized cells is a convenient method for determining the fraction of cells that is infected. Commercially available reagents are suitable for performing such tests (Lee et al., 1994, J. Virol. 68:8254-8264).

In Section 6.2, infra, GSEs were tested for their ability to protect CEM-ss cells from infection with HIV-1 strains SF 2 and IIIB. Uninfected cells were transduced with a LXSN construct containing either an irrelevant DNA or a GSE sequence. Non-transduced cells were eliminated by exposure to the selection agent, G-418. The percentage of p24' cells was determined at specific time points post infection. The results demonstrate that GSEs tested are able to protect a productive HIV-1 infection in susceptible host cells.

5.6. USES OF GSE TO SUPPRESS HIV-1 INFECTION Another aspect of the present invention is to use the isolated GSEs against HIV infection prophylactically or therapeutically. In this connection, GSE operably linked to a regulatory sequence such as a promoter that controls its expression may be transferred in vitro into any HIVsusceptible host cells such as CD4'T cells or hematopoietic progenitor cells such as CD34' cells obtained from bone marrow WO 00/37635 PCT/US99/30187 or mobilized peripheral blood, by any DNA transfer techniques well known in the art such as electroporation, transfection or transduction, followed by transplantation of the cells into a recipient. When the GSE-containing progenitor cells differentiate in vivo, the progeny cells express the GSE and become resistant to HIV.

Alternatively, GSEs may be directly administered in vivo using a gene therapy expression vector. In particular, anti- HIV GSEs can be delivered or transferred into CD4' T cells in both HIV-infected or uninfected individuals to protect against development of HIV infection. GSEs can also be transferred into stromal cells, including macrophages.

Expression vectors derived from viruses such as retroviruses, adeno-associated virus, herpes viruses, or bovine papilloma virus may be used for delivery of a recombinant GSE into the targeted cell population. Methods which are well known to those skilled in the art can be used to construct recombinant viral vectors containing a GSE sequence operably linked to a promoter that controls its expression (Sambrook et al., 1989, Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, N.Y. and Ausubel et al., 1989, Current Protocols in Molecular Biology, Greene Publishing Associates and Wiley Interscience, In a specific embodiment by way of example, GSE sequences were inserted into a retroviral vector. In cases where an adenovirus is used as an expression vector, a GSE sequence may be ligated to an adenovirus transcription-translation control complex, the late promoter and tripartite leader sequence. This chimeric gene may then be inserted in the adenovirus genome by in vitro or in vivo recombination.

Insertion in a non-essential region of the viral genome region El or E3) will result in a recombinant virus that is viable and capable of expressing GSE in infected hosts (Logan Shenk, 1984, Proc. Natl. Acad. Sci. USA 81:3655-3659).

Alternatively, recombinant GSE nucleic acid molecules can be reconstituted into liposomes for delivery to target cells.

WO 00/37635 PCT/US99/30187 Liposomes are spherical lipid bilayers with aqueous interiors.

All molecules that are present in an aqueous solution at the time of liposome formation (in this case, oligonucleotides) are incorporated into this aqueous interior. The liposomal contents are both protected from the external microenvironment and, because liposomes fuse with cell membranes, are efficiently delivered into the cell cytoplasm, obviating the need to neutralize the polynucleotides' negative charge.

Specific initiation signals may also be required for efficient translation of inserted GSE sequences. Exogenous transcriptional control signals, including the ATG initiation codon, must be provided. Furthermore, the initiation codon must be in phase with the reading frame of the GSE sequence to ensure translation of the entire insert. These exogenous translational control signals and initiation codons can be of a variety of origins, both natural and synthetic. The efficiency of expression may be enhanced by the inclusion of appropriate transcription enhancer elements, transcription terminators, etc. (see Bittner et al., 1987, Methods in Enzymol. 153: 516-544).

The isolated GSE sequences suppress HIV activity by either encoding protein or RNA products. The present invention encompasses any such protein product, including fusion proteins, leader peptides and localization signals. In addition, anti-sense RNA, DNA molecules and ribozymes that function to inhibit HIV infection are also within the scope of the invention. Anti-sense RNA and DNA molecules act to directly block the translation of mRNA by binding to targeted mRNA and preventing protein translation. GSEs may be represented by structural RNAs which act as decoys.

Some GSEs may also form triplexes.

Oligodeoxyribonucleotides can form sequence-specific triple helices by hydrogen bonding to specific complementary sequences in duplexed DNA. Formation of specific triple helices may selectively inhibit the replication and/or gene expression of targeted genes by prohibiting the specific binding of functional trans-acting factors.

WO 00/37635 PCT/US99/30187 Polynucleotides to be used in triplex helix formation should be single stranded and composed of deoxynucleotides.

The base composition of these polynucleotides must be designed to promote triple helix formation via Hoogsteen base pairing rules, which generally require sizeable stretches of either purines or pyrimidines to be present on one strand of a duplex. Polynucleotide sequences may be pyrimidine-based, which will result in TAT and CGC triplets across the three associated strands of the resulting triple helix. The pyrimidine-rich polynucleotides provide base complementarity to a purine-rich region of a single strand of the duplex in a parallel orientation to that strand. In addition, polynucleotides may be chosen that are purine-rich, for example, containing a stretch of G residues. These polynucleotides will form a triple helix with a DNA duplex that is rich in GC pairs, in which the majority of the purine residues are located on a single strand of the targeted duplex, resulting in GGC triplets across the three strands in the triplex.

Alternatively, the potential sequences that can be targeted for triple helix formation may be increased by creating a so called "switchback" oligonucleotide. Switchback oligonucleotides are synthesized in an alternating 3'manner, such that they base pair with first one strand of a duplex and then the other, eliminating the necessity for a sizeable stretch of either purines or pyrimidines to be present on one strand of a duplex.

Ribozymes are enzymatic RNA molecules capable of catalyzing the specific cleavage of RNA. The mechanism of ribozyme action involves sequence specific hybridization of the ribozyme molecule to complementary target RNA, followed by endonucleolytic cleavage. Within the scope of the invention are engineered hammerhead motif ribozyme molecules that specifically and efficiently catalyze endonucleolytic cleavage of HIV RNA sequences. GSE represented by antisense RNA showing high affinity binding to target sequences can also be WO 00/37635 PCT/US99/30187 used as ribozymes by addition of enzymatically active sequences known to those skilled in the art.

Both anti-sense RNA and DNA molecules, and ribozymes of the invention may be prepared by any method known in the art.

These include techniques for chemically synthesizing oligodeoxyribonucleotides well known in the art such as solid phase phosphoramidite chemical synthesis. Alternatively, RNA molecules may be generated by in vitro and in vivo transcription of DNA sequences encoding the antisense RNA molecule. Such DNA sequences may be incorporated into a wide variety of vectors which incorporate suitable RNA polymerase promoters such as the T7 or SP6 polymerase promoters.

Alternatively, antisense cDNA constructs that synthesize antisense RNA constitutively or inducibly, depending on the promoter used, can be introduced stably into host cells.

Various modifications to the nucleic acid molecules may be introduced as a means of increasing intracellular stability and half-life. Possible modifications include, but are not limited to, the addition of flanking sequences of ribonucleotides or deoxyribonucleotides to the 5' and/or 3' ends of the molecule or the use of phosphorothioate or 2' 0methyl rather than phosphodiesterase linkages within the oligodeoxyribonucleotide backbone.

Methods for introducing polynucleotides into cells or tissues include the insertion of naked polynucleotide, i.e., by injection into tissue, the introduction of a GSE in a cell ex vivo, for use in autologous cell therapy, the use of a vector such as a virus, retrovirus, phage or plasmid, etc.

or techniques such as electroporation which may be used in vivo or ex vivo.

The GSEs may be formulated and administered through a variety of means, including systemic, localized, or topical administration. Techniques for formulation and administration may be found in "Remington's Pharmaceutical Sciences," Mack Publishing Co., Easton, PA, latest edition. The mode of WO 00/37635 PCT/US99/30187 administration may be selected to maximize delivery to a desired target site in the body.

For systemic administration, route of injection includes, intramuscular, intravenous, intraperitoneal, and subcutaneous.

The polynucleotides of interest are formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks's solution, Ringer's solution, or physiological saline buffer. In addition, the polynucleotides may be formulated in solid or lyophilized form, then redissolved or suspended immediately prior to use.

6. EXAMPLE: ISOLATION AND IDENTIFICATION OF GSE AGAINST HIV-1 6.1. MATERIALS AND METHODS 6.1.1. CONSTRUCTION OF RFE LIBRARY HIV-1 genomic DNA included pBENN7 (Cat. No. 342) derived from 5' half of HIV-IBRu (AIDS Research and Reference Reagent Program, Rockville, MD) (ARRRP), 9B/6R derived from 3' half of HIV-1sF2 and HIV-1HxB2 genomic DNA. One RFE library was constructed using HIV-1BRu and HIV-1sF 2 -derived genomes as the DNA source. A second library was made from HIV-IHxB 2 genomic DNA. The plasmids were partially digested with DNAseI in the presence of manganese (Sambrook et al., 1989, Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, Under these conditions, DNAseI is known to produce mostly double-stranded breaks. The resulting fragments were repaired with the Klenow fragment of DNA polymerase I and T4 polymerase and ligated to synthetic double-stranded adaptors.

The adaptor sequence for the first library contained three ATGs: (SEQ ID NO: 26) For the second library, only one ATG was used: (SEQ ID NO: 27) Both libraries used the same 3' primer containing three stop codons: (SEQ ID NO: 28) WO 00/37635 PCT/US99/30187 Thereafter, the mixture was digested with BamHI and EcoRI, column purified and ligated to the retroviral vector LXSN (Miller and Rosman, 1989, BioTechniques 7:980-990) cut with EcoRI and BamHI. Alternatively, the fragments were ligated to the vector LXSNgfr, which was constructed by replacing the neomycin resistance gene of LXSN with a truncated low affinity nerve growth factor receptor gene. The ligation mixture was transformed into E. coli. The total plasmid was purified from -100,000 recombinant clones. The size distribution of the cloned fragments was tested by PCR amplification using primers derived from the vector sequences adjacent to the adaptors.

6.1.2. CELL LINES AND REAGENTS The OM10.1 cells are available from the American Type Culture Collection, Rockville, MD as CRL 10850 (Butera, United States Patent No. 5,256,534). OM10.1 cells were a chronically infected promyelocytic clone of HL-60 which contained a single copy of the HIV-1 8 Ru isolate. The CEM-ss cells are available from the ARRRP as Cat. No. 776. The cells were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37 0 C and 5% CO 2 The amphotropic packaging cell line BING was maintained in DMEM supplemented with 10% FBS at 37 0 C and

CO

2 Persistently HIV-infected cells, HUT78/HIV-1 5

F

2 and H9/HIV-11lIs were obtained from ARRRP and used to prepare HIV- 'SF2 and HIV-1 1 IIB viral stocks.

The anti-CD4 antibodies, Q4120PE (Sigma, St. Louis, MO) and L120 (Becton Dickinson Immunocytometry Systems, San Jose, CA), and anti-p24 (KC-57 FITC, Coulter, Hialeah, FL) antibodies were purchased. TNF-a was obtained from Boehringer Mannheim. G418 was purchased from Gibco/BRL as neomycin (Gaithersburg, MD).

6.1.3. TRANSFECTION AND TRANSDUCTION The plasmid DNA prepared according to the method of Section 6.1.1. supra, was transfected into the packaging cell WO 00/37635 PCT/US99/30187 line, BING, using a standard calcium phosphate method. For LXSN-based libraries and GSEs, the packaging cells were cocultivated with OM10.1 cells or CEM-SS cells for 2-3 hours.

Three cocultivations were used at 24, 48 and 72 hours after transfection. Cells were then grown under neomycin selection for two weeks. The surviving cells were purified using "FICOLL" and grown in neomycin-free media before any further manipulations. A different method was used for LXSNgfr based libraries and GSEs. Filtered retroviral supernatants from BING cells (24 and 48 hour virus) were used to infect the target cells by centrifugation at 1200xg for 90 minutes. One week later, cells were stained with a NGFR monoclonal antibody and the transduced cells represented by NGFR positive population were sorted using a FACS Vantage (Becton Dickinson) equipped with a 488 nm argon laser. Cells were cultured in nedia and checked for NGFR prior to use.

6.1.4. IMMUNOFLUORESCENCE AND FLOW CYTOMETRY For the selection of CD4 or NGFR cells, 10 7 cells were washed twice with Assay Buffer (500 ml PBS, 1 ml of 0.5 mM of EDTA at pH 8, 0.5 ml of 10% sodium azide and 10 ml of fetal bovine serum), and resuspended in 500 il PBS to which 50 .l of an anti-CD4 antibody, Q4120PE or L120, or PE-conjugated anti- NGFR antibody 20.4 (ATCC) was added. After incubation at 4 0

C

for 30 min., 5 ml of Assay Buffer was added and the cells centrifuged at 1200 rpm for 4 min. The cells were washed twice with Assay Buffer before sorting by FACS. The aforementioned procedure was performed under sterile conditions.

In order to determine p24 expression in HIV-infected cells, the cells were first washed twice with Assay Buffer.

About 106 cells were suspended in 100 pl Assay Buffer, mixed with 2 ml of Ortho PermeaFix Solution (Ortho Diagnostics, Raritan, NJ), and incubated for 40 min. at room temperature.

After centrifugation at 1200 rpm for 4 min. at room temperature, the cells were resuspended in 2 ml Wash Buffer (500 ml PBS, 25 ml fetal bovine serum, 1.5% bovine serum WO 00/37635 PCT/US99/30187 albumin and 0.0055% EDTA) for 10 min. at room temperature.

After centrifugation, the cells were resuspended in 50 Al Wash Buffer and mixed with 1:500 dilution of an IgG 2 a antibody for min. at room temperature, followed by incubation with 5-10 Al of anti-p24 antibody (KC57-FITC, Coulter, Hialeah, FL) for min. at room temperature. The cells were then washed twice with Wash Buffer and analyzed by flow cytometry.

6.1.5. SELECTION OF GSE IN OM10.1 CELLS The transduced populations of the OM10.1 cells (GSE library or insert free vector) were washed once with PBS, then induced with 0.1 ng/ml of TNF-a (Boehringer Mannheim, Indianopolis, IN) in RPMI supplemented with 10% FBS at a density 5x10 5 cells/ml. After 24 hours, cells were stained with a PE-conjugated anti-CD4 monoclonal antibody. Propidium iodide was added to a final concentration of 10 Ag/ml immediately prior to sorting. The propidium iodide negative, CD4 positive population was sorted by flow cytometry. The cells were lysed and the genomic DNA was purified. Inserts were amplified by PCR using vector-derived primers, the mixture was digested with BamHI and EcoRI, column purified, ligated to BamHI/EcoRI digested vector, and transformed into E. coli. Purified DNA from transformants were either used as a pool for subsequent rounds of selection and/or individually isolated and sequenced using the ALF DNA Sequencer (Pharmacia LKB, Piscataway, NJ).

6.1.6. PRODUCTIVE INFECTION SELECTION OF GSE Transduced CEM-ss cells (GSE library or insert free vector) were infected with HIV-,m 1 a at a tissue culture infectious doses (TCID 50 of 3000 per 106 cells in the presence of polybrene. At 9 days post infection, 107 cells were stained for CD4 and p24. The p24 negative, CD4 positive population was sorted. Genomic DNA purification, insert amplification and subcloning were performed as described above.

6.1.7. RECOVERY OF GSE AND SEQUENCE ANALYSIS Genomic DNA was isolated from the selected population of OM10.1 cells or CEM cells harboring putative GSE by WO 00/37635 PCT/US99/30187 resuspending the cell pellet in 0.1% Triton X-100, proteinase K in Ix PCR buffer, incubating at 55°C for 1 hour, and boiling for 10 minutes. Genomic DNA was used for PCR amplification using vector-derived primers, cloned into the LXSN vector, and transformed into E. coli using techniques well known in the art. Individual plasmids were purified from E. coli clones using QIAGEN plasmid kits. Inserts were sequenced by the dideoxy procedure (AutoRead Sequencing Kit, Pharmacia Biotech) and run on a Pharmacia LKB A.L.F. DNA sequencer. Sequences were analyzed using the DNASTAR program.

6.2. RESULTS The HIV-1 life cycle consists of two distinct stages: productive or acute infection and latency. An ideal HIV inhibitor should be effective against both stages.

Accordingly, two selection approaches of HIV-1 GSEs were developed. The first approach, designed to inhibit virus induction in latently infected cells, was based on the unique properties of OM10.1 cells. For selection of GSEs in OM10.1 cells, an HIV-1 RFE library was constructed from plasmids containing the genome of the virus. Following transfection of the entire library into a packaging cell line, virus was transferred into OM10.1 cells by co-cultivation. The virallytransduced cells were selected in culture medium containing G- 418 to ensure the retention of the viral vector.

When the transduced OM10.1 cells were treated with TNF-a and stained with an antibody specific for the cell surface molecule CD4, a rapid loss of CD4 expression was observed in about 90% of the cells (Figure In contrast, approximately 99% of the uninduced OM10.1 cells retained CD4 expression. It is believed that activation of the latent virus in OM10.1 cells by TNF-a led to the production of viral protein gp160/120, which bound to cytoplasmic CD4, thereby preventing its cell surface translocation. A diminution of CD4' OM10.1 cells also correlated with an increased production of viral protein p24 in the cells following TNF-a induction (Figure 2).

Based on these findings, expression of a GSE capable of interfering with induction was expected to result in the WO 00/37635 PCT/US99/30187 retention of surface CD4 by OM10.1 cells. The small number of residual CD4 cells were stained with an anti-CD4 antibody and sorted by FACS. After the cells were expanded in culture, the individual GSE polynucleotides were recovered by PCR amplification and their nucleotide sequences determined.

The second selection scheme was designed to select GSEs capable of inhibiting productive HIV infection. Replication of HIV.in susceptible cells is associated with accumulation of intracellular p24, concomitant with down-modulation of surface CD4. For productive infection selection, an HIV-1 RFE library was constructed from plasmids containing the genome of the virus. The entire library was transfected into CEM-ss cells.

The cells were infected with HIV-1,ma and sorted by flow cytometry for CD4 positive, p24 negative cells.

RFE libraries from various HIV-1 isolates were cloned into retroviral vectors. A library from isolates HIV-lBRU and HIV-1SE 2 in LXSN vector was used in OM10.1 selection. A library from HIV-1HXB2 in LXSN vector was used in productive infection selection. A third library from HIV-1HXB 2 in LXSNgfr vector was used in two independent experiments in OM10.1 cells. Each RFE library was transferred into the target cells (OM10.1 or CEM-ss) and two rounds of selection were performed.

The reproducibility of the system was demonstrated by independent transfers and selections of the same RFE library.

After the second round of selection in OM10.1 cells, 40-50% of the elements were from a few short regions of the HIV-1 genome, indicating selection for these sequences. Sequence comparison of individual elements enriched in all selections revealed 7 clusters of overlapping sequences, 5 in the sense orientation and 2 in the antisense orientation (Figure 3).

Two clusters are from areas of the HIV-1 genome where two or more viral genes overlap (vpr/tat, rev/tat, env/nef). Some elements were nearly identical to those isolated in the productive infection selection. Thus, three clusters (the nef sense cluster, the rev/tat sense cluster, and the vpr/tat antisense cluster) were identified-in all libraries and selections. A cluster of sense-oriented elements from the WO 00/37635 PCT/US99/30187 reverse transcriptase (RT) gene was found only in the productive infection selection, presumably because reverse transcription preceded integration. SEQ ID NOS: 1-25 present nucleotide sequences of the selected polyncleotides which corresponded to GSEs in either sense or antisense orientation.

More specifically, GSE IGX-149 in SEQ ID NO:1 is in the gag (p24) sense orientation. IGX 104 (SEQ ID NO:2), IGX-109 (SEQ ID NO:3) and IGX-102 (SEQ ID NO:4) are in the pol (reverse transcriptase) sense orientation. IGX-111 (SEQ ID NO:5), IGX- 013 (SEQ ID NO:6), IGX-018 (SEQ ID NO:7), IGX-117 (SEQ ID NO:8), IGX-217 (SEQ ID NO:9) and IGX-050 (SEQ ID NO:10) are in vpr/tat sense orientation. IGX-120 (SEQ ID NO:11), IGX-108 (SEQ ID NO:12), IGX-031 (SEQ ID NO:13) and IGX-201 (SEQ ID NO:14) are in RRE antisense orientation. IGX-103 (SEQ ID and IGX-171 (SEQ ID NO:16) are in rev/tat sense orientation. IGX-222 (SEQ ID NO:17), IGX-003 (SEQ ID NO:18), IGX-219 (SEQ ID NO:19), IGX-210 (SEQ ID NO:20), IGX-221 (SEQ ID NO:21) and IGX-151 (SEQ ID NO:22) are in env/nef antisense orientation. IGX-078 (SEQ ID NO:23), IGX-121 (SEQ ID NO:24) and IGX-140 (SEQ ID NO:25) are in nef sense orientation.

The fact that GSEs with overlapping sequences from narrowly defined regions of the HIV-1 genome were isolated in different selection systems using different HIV-1 isolates indicates that these GSEs are from functionally important and conserved regions of the viral genome. Surprisingly, clusters of GSEs from previously untargeted regions (RT and nef) were also isolated.

GSEs from the first OM10.1 selection were further tested for their ability to prevent HIV-1 induction by TNF-a. After induction, negative controls (OM10.1 cells and OM10.1 cells with the LXSN vector) revealed background levels of 10% CD4 positive cells (Figure Two additional negative controls containing anonymous DNA inserts from plasmid vectors (#34 and #220) were 8-11% CD4 positive. However, individual GSE showed a constant and reproducible inhibitory effect by allowing 18- 26% of the transduced cells to retain CD4 upon induction with WO 00/37635 PCT/US99/30187 TNF-a (Figure In contrast, OM10.1 cells containing the transdominant mutant, RevM10 (Malin et al., 1989, Cell 58:205), were not effective at inhibiting induction of virus from latency.

GSEs isolated by OM10.1 selection were tested for their ability to protect uninfected human T cells from a productive HIV-1 infection. Plasmids containing each of these sequences were transferred into CEM-ss cells followed by G418 selection.

The Rev transdominant mutant, RevM10 and a LXSN vector containing an irrelevant piece of plasmid DNA were used as controls. The G418 resistant cells were 99% CD4 and were then infected with low titers (TCID 50 of 500-1000) of HIV-ls, 2 or high titers (TCID, 5 of 3000) of HIV-1lre. The cells were removed at various days after infection, and stained with a fluorescinated-anti-p24 antibody as an indicator of HIV infection.

Upon infection with HIV-1sF 2 a significant delay was observed in the development of the productive infection as determined by intracellular p24 staining (Figure 5A). GSEs isolated in productive infection selection had sequences overlapping with those from the OM10.1 selection and had similar effects upon viral challenge. The RT cluster of elements isolated in productive infection selection showed inhibitory effects when challenged with HIV- 1 1 nB (Figure In all infections, the GSEs demonstrated similar or better inhibitory effects than the transdominant mutant, RevM10, a known inhibitor of HIV-1 replication in T cells.

In conclusion, a large number of GSEs have been isolated from the HIV-1 genome based on their ability to maintain CD4 expression in OM10.1 cells after activation of latent HIV by induction with TNF-a or based on their ability to inhibit productive HIV infection in CEM-ss cells. The isolated GSEs contain nucleotide sequences in both sense and anti-sense orientations, and are mapped to different regions of the HIV-1 genome. Several elements corresponding to portions of the integrase, Nef and Rev/Tat genes are able to suppress HIV-1 infection of T cells by reducing p24 levels in infected cells.

WO 00/37635 PCT/US99/30187 Such polynucleotides are useful in protecting the infection by and/or suppressing the replication of HIV-l in human host cells.

The present invention is not to be limited in scope by the exemplified embodiments, which are intended as illustrations of individual aspects of the invention. Indeed, various modifications for the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description and accompanying drawings. Such modifications are intended to fall within the scope of the appended claims.

All publications cited herein are incorporated by reference in their entirety.

EDITORIAL NOTE APPLICATION NUMBER 21954/00 The following Sequence Listing pages 1 to 6 are part of the description. The claims pages follow on pages "29" to "36".

WO 00/37635 PCT/US99/30187 SEQUENCE LISTING <110> Dunn, Stephen Holzmayer, Tanya <120> Genetic Suppressor Elements Against Human Immunodeficiency Virus <130> 99-283-A <140> PCT/US99/ <141> 1999-12-17 <150> US 09/218,755 <151> 1998-12-22 <160> 28 <170> PatentIn Ver. <210> 1 <211> <212> DNA <213> Human immunodeficiency virus <400> 1 atgacaaata atccacctat cccagtagga gaaatttata aaagatggat aatcctggga <210> 2 <211> 86 <212> DNA <213> Human immunodeficiency virus <400> 2 gggttaaaaa agaaaaaatc agtaacagta ctggatgtgg gtgacgcata tttttcagtt cccttagatg aagacttcag gaaata 86 <210> 3 <211> 77 <212> DNA <213> Human immunodeficiency virus <400> 3 aaaaagaaaa aatcagtaac agtactggat gtgggtgacg catatttttc agttccctta gatgaagact tcaggaa 77 <210> 4 <211> 67 <212> DNA <213> Human immunodeficiency virus <400> 4 cagtaacagt actggatgtg ggtgacgcat atttttcagt tcccttagat gaagacttca ggaaata 67 WO 00/37635 WO 0037635PCTIUS99/30187 <210> <211> 216 <212> DNA <213> Human immunodeficiency virus <400> gaattctgca acaactgctg tttatccatt tcagaattgg gtgtcgacat agcagaatag gcgttactca acagaggaga gcaagaaatg gagccagtag atcctagact agagccctgg 120 aagcatccag gaagtcagcc taaaactgct tgtaccactt gctattgtaa aaagtgttgc 180 tttcattgcc aagtttgttt cacaacaaaa gcctta 216 <210> 6 <211> 144 <212> DNA <213> Human immunodeficiency virus <400> 6 gaattctgca acaactgctg tttatccatt tcagaattgg gtgtcgacat agcagaatag gcgttactca acagaggaga gcaagaaatg gagccagtag atcctagact agagccctgg 120 aagcatccag gaagtcagcc taaa 144 <210> 7 <211> 184 <212> DNA <213> Human immunodeficiency virus <400> 7 gaattctgca acaactgctg tttatccatt tcagaattgg gtgtcgacat agcagaatag gcgttactca acagaggaga gcaagaaatg gagccagtag atcctagact agagccctgg 120 aagcatccag gaagtcagcc taaaactgct tgtaccactt gctattgtaa aaagtgttgc 180 ttt c 184 <210> 8 <211> 94 <212> DNA <213> Human immunodeficiency virus <400> 8 gaattctgca acaactgctg tttatccatt tcagaattgg gtgtcgacat agcagaatag gcgttactca acagaggaga gcaagaaatg gagc 94 <210> 9 <211> 92 <212> DNA <213> Human immunodeficiency virus <400> 9 gaattctgca acaactgctg tttatccatt tcagaattgg gtgtcgacat agcagaatag gcgttactca acagaggaga gcaagaaatg ga 92 <210> <211> 179 <212> DNA <213> Human immunodeficiency virus WO 00/37635 WO 0037635PCTIUS99/301 87 <400> acaactgctg tttatccatt tcagaattgg gtgtcgacat agcagaatag gcgttactca acagaggaga gcaagaaatg gagccagtag atcctagact agagccctgg aagcatccag 120 gaagtcagcc taaaactgct tgtaccactt gctattgtaa aaagtgttgc tttcattgc 179 <210> 11 <211> 104 <212> DNA <213> Human immunodeficiency virus <400> 11 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat acca 104 <210> 12 <211> 69 <212> DNA <213> Human immunodeficiency virus <400> 12 cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgca 69 <210> 13 <211> 83 <212> DNA <213> Human immunodeficiency virus <400> 13 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atg 83 <210> 14 <211> 91 <212> DNA <213> Human immunodeficiency virus <400> 14 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac a 91 <210> <211> 124 <212> DNA <213> Human immunodeficiency virus <400> ggcccgacgg aatcgaagaa gaaggtggag agagacagag acagatccgt tcgattagtg tatggattct tagcacttat ctgggaagat ctgcggagcc tgtgcctctt cagctaccgc 120 cgc t 124 <210> 16 <211> 71 <212> DNA <213> Human immunodeficiency virus WO 00/37635 WO 0037635PCTIUS99/301 87 <400> 16 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc c 71 <210> 17 <211> 339 <212> DNA <213> Human immunodeficiency virus <400> 17 tgtattgcta atctgctgct actttttgac ttctaggtat tcccctcagc cctgactcca cttgtgattg ggctcagctc cacttgccac gtggcgaata tactgctatg atactgtagg ctccatgttt gtctcattct ccatcttata gctctacaag gctgtggcat agattggagg ttccaggtct ttcccttaca gcaaaatcct ctccttgtac tgagcaagct aatatttga cgagatgctg ctcccacccc gtaggccatc caaccacact ttccaagccc tgtcttattc tacttctata accctatctg aacagcacta ttctttagtt 120 180 240 300 339 <210> 18 <211> 339 <212> DNA <213> Human immunodeficiency virus <400> 18 caggcacaag aggtc tcgag cttacagtag aatcctttcc ttgtactact caagctaaca cagcattggt atgctgctcc gccatccaac aagccctgtc tctataaccc gcactattct agctgctgta caccccatct cacactactt ttattcttct tatctgtccc ttagttcctg ttgctacttg gctgctggct tttgaccact aggtatgtgg ctcagctact actccaata tgattgctcc cagctcgtct tgccacccat cgaatagctc gctatggctg atgtttttcc cattctttcc cttatagcaa tacaagctcc tggcattgag 120 180 240 300 339 <210> 19 <211> 103 <212> DNA <213> Human immunodeficiency virus <400> 19 aaatcctttc caagccctgt cttattcttc taggtatgtg gcgaatagct ctacaagctc cttgtactac ttctataacc ctatctgtcc cctcagctac tgc 103 <210> <211> 112 <212> DNA <213> Human immunodeficiency virus <400> actttttgac cacttgccac ccatcttata gcaaaatcct ttccaagccc tgtcttattc ttctaggtat gtggcgaata gctctacaag ctccttgtac tacttctata ac 112 <210> 21 <211> 113 <212> DNA <213> Human immunodeficiency virus <400> 21 WO 00/37635 PCTIUS99/301 87 tcctcctctt gtgcttctag ccaggcacaa gcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc caggtctcga gatgctgctc ccaccccatc tgc 113 <210> 22 <211> 339 <212> DNA <213> Human immunodeficiency virus <400> 22 tgtattgcta cttgtgattg ctccatgttt ttccaggtct cgagatgctg ctcccacccc atctgctgct ggctcagctc gtctcattct ttcccttaca gtaggccatc caaccacact 120 actttttgac cacttgccac ccatcttata gcaaaatcct ttccaagccc tgtcttattc 180 ttctaggtat gtggcgaata gctctacaag ctccttgtac tacttctata accctatctg 240 tcccctcagc tactgctatg gctgtggcat tgagcaagct aacagcacta ttctttagtt 300 cctgactcca atactgtagg agattggagg aatatttga 339 <210> 23 <211> 91 <212> DNA <213> Human immunodeficiency virus <400> 23 tgggagcagt atctcgagac ctggaaaaac atggagcgat cacaagtagc aatacagcag ctactaatgc tgattgtgcc tggctagaag c 91 <210> 24 <211> 82 <212> DNA <213> Human immunodeficiency virus <400> 24 cgagacctgg aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgct tgtgcctggc tagaagcaca ag 82 <210> <211> 79 <212> DNA <213> Human immunodeficiency virus <400> aagtagcaat acagcagcta ccaatgctgc ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtc 79 <210> 26 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 26 gaattcaagc ttatggatgg atg 23 <210> 27 WO 00/37635 PCT/US99/30187 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 27 gattcagctt gccgcaccat ggct 24 <210> 28 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Primer <400> 28 ggatccatcg attcactcac tca 23

Claims (54)

1. An isolated polynucleotide, consisting of a nucleotide sequence recited in any one of SEQ ID NOS: 1 to 25, or comprising a fragment of a gene comprising a nucleotide sequence recited in any one of SEQ ID NOS: 2 to 6, 8 to 11, 14, 15, or 17 to 25, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

2. An isolated polynucleotide consisting of a nucleotide sequence recited in any one of SEQ ID NOS: 1 to

3. An isolated polynucleotide comprising a fragment of a gene comprising a 0o nucleotide sequence recited in any one of SEQ ID NOS: 2 to 6, 8 to 11, 14, 15, or 17 to wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

4. An isolated polynucleotide consisting of a nucleotide sequence that is fully complementary to a nucleotide sequence of any one of SEQ ID NOS: 1 to

5. An isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO:1, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

6. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:2, wherein said nucleotide sequence is operably 20 linked to a regulatory sequence, and wherein expression of said polynucleotide in a host S cell suppresses infection by HIV-1. o 7. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:3, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

8. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:4, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. 30 9. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:5, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. [R:\LIBVV]031 An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:6, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

11. An isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO:7, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

12. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:8, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

13. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:9, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

14. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:10, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. 20 15. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:11, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

16. An isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO:12, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

17. An isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO: 13, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. 30 18. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:14, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. [R:\LIBVV]03165.doc:sxc

19. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:15, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

20. An isolated polynucleotide consisting of a nucleotide sequence of SEQ ID NO: 16, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

21. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:17, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

22. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:18, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

23. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:19, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. 20 24. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:20, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:21, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

26. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:22, wherein said nucleotide sequence is operably 30 linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

27. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:23, wherein said nucleotide sequence is operably [R:\LIBVV]03165.doc:sxc 32 linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

28. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:24, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

29. An isolated polynucleotide comprising a fragment of a gene comprising a nucleotide sequence of SEQ ID NO:25, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host 0o cell suppresses infection by HIV-1. The isolated polynucleotide of any one of claims 1 to 29 that is contained in a recombinant vector.

31. The isolated polynucleotide of any one of claims 1 to 29 that is contained in an expression vector. Is 32. A genetically engineered host cell that contains the vector of claim 30 or claim 31.

33. The genetically engineered host cell of claim 32 that expresses said nucleotide sequence as a peptide.

34. The genetically engineered host cell of claim 32 that expresses said nucleotide 20 sequence as an RNA molecule. A method of inhibiting HIV infection, the method comprising the step of :contacting a cell with the polynucleotide of any one of claims 1 to 29, thereby inhibiting HIV replication in said cell.

36. A method of treating HIV infection in a subject, the method comprising the step of administering to said subject a therapeutically effective amount of the polynucleotide of any one of claims 1 to 29. i.

37. An isolated polynucleotide, consisting of a first nucleotide sequence that hybridizes under highly stringent conditions to a second nucleotide sequence that is complementary to any one of SEQ ID NOS: 2 to 5, 8, 9, 11, 14, 15 or 17 to 25, wherein 30 said first nucleotide sequence is operably linked to a regulatory sequence, and wherein *expression of said polynucleotide in a host cell suppresses infection by HIV-1.

38. The isolated polynucleotide of claim 37 that is contained in a recombinant vector. [R:\LIBVV]03165.doc:sxc

39. The isolated polynucleotide of claim 37 that is contained in an expression vector. A genetically engineered host cell that contains the vector of claim 38 or claim 39.

41. The genetically engineered host cell of claim 40 that expresses said nucleotide sequence as a peptide.

42. The genetically engineered host cell of claim 40 that expresses said nucleotide sequence as an RNA molecule.

43. A method of inhibiting HIV infection, the method comprising the step of contacting a cell with the polynucleotide of claim 37, thereby inhibiting HIV replication in said cell.

44. A method of treating HIV infection in a subject, the method comprising the step of administering to said subject a therapeutically effective amount of the polynucleotide of claim 37. Is 45. Use of a therapeutically effective amount of the polynucleotide of any one of claims 1 to 29 for the manufacture of a medicament for treating HIV infection in a subject.

46. Use of a therapeutically effective amount of the polynucleotide of claim 37 for the manufacture of a medicament for treating HIV infection in a subject.

47. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:2, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

48. An isolated polynucleotide comprising a fragment of a gene consisting of a 25 nucleotide sequence of SEQ ID NO:3, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

49. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:4, wherein said nucleotide sequence is operably 30 linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

50. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:5, wherein said nucleotide sequence is operably [R:\LIBVV]03353.doc:sxc linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

51. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:6, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

52. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:8, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host to cell suppresses infection by HIV-1.

53. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:9, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

54. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:10, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:l1, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host S: cell suppresses infection by HIV-1.

56. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:14, wherein said nucleotide sequence is operably 25 linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

57. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:15, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host 30 cell suppresses infection by HIV-1.

58. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:17, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. [R:\LIBVV]03353.doc:sxc

59. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:18, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

60. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:19, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

61. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:20, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

62. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:21, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

63. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:22, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

64. An isolated polynucleotide comprising a fragment of a gene consisting of a nucleotide sequence of SEQ ID NO:23, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

65. An isolated polynucleotide comprising a fragment of a gene consisting of a S" nucleotide sequence of SEQ ID NO:24, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1.

66. An isolated polynucleotide comprising a fragment of a gene consisting of a 30 nucleotide sequence of SEQ ID NO:25, wherein said nucleotide sequence is operably linked to a regulatory sequence, and wherein expression of said polynucleotide in a host cell suppresses infection by HIV-1. [R:\LIBVV]03353.doc:sxc

67. An isolated polynucleotide substantially as hereinbefore described with reference to any one of the examples. Dated 6 January, 2004 PPD Development, LP Patent Attorneys for the Applicant/Nominated Person SPRUSON FERGUSON [R:\LIBVV]03353.doc:sxc