AU742747B2 - Peptide turn mimetics - Google Patents

Description

wn OQ/4Q1 lr A /nrn "b O I O -TAf, 1- i It'tUYIUU4.U

TITLE

"PEPTIDE TURN MIMETICS" FIELD OF THE INVENTION THIS INVENTION relates to new compounds designed to be peptide turn mimetics, and to new compounds useful for the synthesis of peptide mimetics, especially turn mimetics. Peptide mimetics are used to reproduce the important structural and functional elements contained in a bio-active peptide sequence principally in order to develop novel pharmaceuticals with increased binding affinity, selectivity, stability and/or oral bioavailability compared to the bio-active peptide.

BACKGROUND OF THE INVENTION Reverse turns (beta and gamma turns and beta bulages) are localised on the protein surface (Kuntz, 1972) and are of importance in protein interactions (Rose et al., 1985; Chalmers and Marshall. 1995) (and references contained therein). In addition reverse turns are important structures of peptide hormones and other biologically active peptides and cyclic peptides.(Giannis and Kolter, 1993; Olson et al., 1993; Kessler et al., 1995) Peptide mimetics and peptide turn mimetics have as their object the replacement of a peptide sequence (a peptide turn) with a new compound which retains the elements essential for biological activity, thereby enabling or facilitating the development of novel pharmaceuticals devoid of the inherent problems of peptides namely flexibility and poor pharmacodynamics. The essential elements for biological activity are thought to be the peptide sidechain groups (Farmer and Ariens, 1982: Ball and Alewood, 1990), therefore a peptide mimetic should include the side chain groups to have the best chance of retaining biological activity.

A peptide mimetic may then take the form of a framework for displaying sidechain groups in an appropriate arrangement.

The majority of reverse turns are beta turns. The generally accepted definition of the beta turn is a sequence of four residues where the distance between the alpha carbons of residue and residue (i+3) WO 99/48913 PCT/AU99/00207 2 (defined as d) is less than 7A, and the central residues i+2) are nonhelical.(Lewis et al., 1973) The general structure is shown below and includes the phi and psi backbone dihedral angles that are used to describe the conformation of the peptide backbone. A schematic conversion of the beta turn to a beta turn mimetic is also shown the peptide backbone is here replaced by an undefined framework.

0 R2\

R

2 H f R C N0

R-

HHN 0 Mimetic Framework R O N R RR' C'(i d<7A C-(i+3) General structure of a hydrogen bonded 1-turn.

The four backbone dihedral angles traditionally used in turn classification are indicated, and also the position of the 7A upper distance cutoff for dused for the definition of 1-turns.

A schematic representation of a beta turn mimetic the peptide backbone has been replaced by an alternative chemical framework, represented here by a rectangle The gamma turn is generally defined by the presence of a hydrogen bond between C=O and N-H to form a pseudo seven membered ring as illustrated below (Milner-White, 1988). Where the equivalent hydrogen bond is present in a beta turn a pseudo ten membered ring is formed.

3 R3 cZ 0

HN

JJ

General structure of a 7 -turn, defined by the presence of a hydrogen bond between the C=0 of the residue and the N-H of the residue, as indicated.

WO 99/48913 PCT/AU99/00207 3 The chemical synthesis of a framework having four independent chiral groups each with a wide range of possible functionality (for example, a beta turn mimetic) is a very significant synthetic challenge (Nakanishi and Kahn, 1996) as illustrated by the the fact that most proposed beta turn mimetics either do not provide for the incorporation of any sidechain functionality, or provide for a limited range of functionality, and at a limited number of positions. Reference may be made to reviews by Ball and by Holzemann for illustration of these points (Ball and Alewood, 1990; Holzemann, 1991; Holzemann, 1991). In the case of mimetics that do provide for the incorporation of sidechain functionality, the syntheses are often complex and lengthy, and most seriously may require a different synthetic method for different sidechain sequences (i.e.

the synthetic method is not generic). For example, in the work of Callahan, Huffman and Newlander on gamma turn mimetics the synthetic method varied depending on the sidechain sequence required a 10 step sequence for a Gly-Phe-Leu mimetic, 13 steps for Phe-Gly-Val and 21 steps for Ala-Phe-Ala (Huffman et al., 1988; Callahan et al., 1992; Newlander et a/.,1993). Given that the possible combinations of three residue sequences of the 20 natural amino acids is 8000 (20x20x20), and 160,000 for the four residue beta turn sequence, such non-generic methods are of limited use. The methods of Callahan and Huffman were further hampered by a lack of chiral control, as are most methods in the art.

In the development of peptide turn mimetics a further important issue is the reproduction of the variety of different turn conformations, particularly of the beta turn. Several different methods of describing turn conformation have been proposed, the traditional method having several turn types based on the backbone dihedral angles of the and residues i.e. I, II, II', III, IIl', IV, V, Via, VIb, VII and VIII, with even this diversity of types being insufficient to adequately describe turn conformations.(Richardson, 1981; Wilmont and Thornton, 1990; Ball WO 99/48913 PCT/AU99/00207 4 et al., 1993) No single mimetic framework can accurately mimic this diversity of turns; a selection of mimetic frameworks is required.

The problems encountered in the development of peptide turn mimetic syntheses are discussed in a review by Kahn (Kahn, 1993) and reference may also be made to a review article entitled "Design of Peptidomimetics" (Nakanishi and Kahn, 1996) which discusses aspects of mimetic design and developments regarding peptide mimetics.

The uses of reverse turn mimetics (and peptides or other compounds containing reverse turn mimetics) in drug development have been described in the art, notably in publications by Kahn and co-workers (Kahn, 1996; Nakanishi and Kahn, 1996; Qabar et a/.,1996) and references contained therein. An important example of the application of reverse turn mimetics is the production of mimetics of known biologically active cyclic peptides (typically penta- or hexapeptides), as illustrated by Hirschmann and co-workers with D-D-glucose based mimetics.(Hirschmann et al.,1992; Hirschmann et al., 1993) Other beta turn mimetics having biological activity are known in the art. For example, U.S. Patent 4535169 discloses a method for the synthesis of beta turn mimetics which can incorporate a functional substitution for the sidechain (only), and Krystenansky et al. disclose a leucine enkephalin mimetic based on this method which had analgesic activity one third the potency of morphine (Krstenansky et al., 1982).

Reference may also be made to U.S. Patents 5475085 and 5618914 and International Publication WO96/22304 (all Kahn, M) which describe methods for the synthesis of a range of reverse turn mimetics.

These mimetics are all produced by a modular synthesis technique (that may be applied to solid phase synthesis) which involves amino acid derivatives and various dipeptide azetidinones synthesised by a variety of techniques. An important common step in all of the syntheses of these mimetics is the cyclisation reaction which involves the azetidinone as activated ester component. Conformational variation is introduced to these mimetics by the inclusion of a variable component in the ring WO 99/48913 PCT/AU99/00207 of the cyclic turn mimetics. It should be noted that with two exceptions (the parent mimetics which have X=NH and have a ten or eleven membered ring) the beta turn mimetics produced by these methods have ring sizes of twelve members and above. Such large rings allow many conformations with d>7A, the mimetic conformations are therefore biased away from the accepted definition of a beta turn (d less than 7A), or more importantly the conformations are biased away from the most common reverse turn conformations which have d in the range of 4.5A to 6A (Rose et al., 1985; Gardner et al.,1993). Enkephalin mimetics have been made (Gardner et al., 1993) and also mimetics of a loop of CD4 that inhibit binding of HIV gp120 and infection of human lymphocytes (Chen et al., 1992). The synthetic methods described for the majority of these mimetics appear to be limited with respect to the possible functionality at the and positions, and indeed no mimetic with any functionality at the position (other than -H glycine no sidechain) appears to have been described at this time.

Reference may also be made to International Publication W097/15577 (Kahn, M) which describes the synthesis of bicyclic reverse turn mimetics and chemical libraries containing such reverse turn mimetics. While concise, the synthetic methods do not provide for control of chirality at all positions, and the degree of sidechain function generality is questionable at two of the four positions. Furthermore the structure of the mimetics means they are not able to be easily incorporated in a peptide sequence, nor do they reproduce the relative positioning of the sidechain groups in the ideal manner (each sidechain attachment position should ideally be separated by three covalent bonds, as in a peptide).

Reference may also be made to the turn mimetics of Virgilio et al. (Valle et al., 1989; Virgilio and Ellman, 1994; Virgilio et al., 1996) that incorporate functionality at the and positions (but not the position), and that do not allow for incorporation of the mimetic in a peptide sequence no amino and carboxy terminal groups in addition to the sidechains are present).

WO 99/48913 PCT/AU99/00207 6 Reference may be made to U.S. Patents 5438188 and 5470849 (Callahan and Huffman) that describe biologically active compounds containing gamma turn mimetics, providing further illustration of the general utility of reverse turn mimetics.

Reference may also be made to International Publication W095/25120 that describes the use of turn mimetics in the synthesis of peptide vaccines for generating a protective immune response in warm blooded animals.

In the methods and mimetics of the aforementioned references several common problems are evident: limited numbers of sidechains are able to be reproduced, there is limited control of chirality in the syntheses and a limited range of sidechain functions could be included. In addition, many of the syntheses of turn mimetics described are relatively long and complex, even when not all the sidechain functions are included, for example the syntheses of certain enkephalin mimetics were in the range of approximately 15 to 21 steps (Gardner et al., 1993).

There is therefore still a need in the art for peptide mimetics that can incorporate a wide range of sidechain functions in all positions, that can be readily synthesised with control of chirality, and that have a wide range of conformations corresponding to those found in native peptides.

OBJECT OF THE INVENTION It is the object of the invention to provide novel compounds useful as, and useful for the synthesis of, conformationally constrained mimetics of biologically active peptides and proteins (peptide mimetics).

In particular, the invention provides new compounds and methods for the synthesis of new peptide reverse turn mimetics that can display a wide range of sidechain functions at all sidechain positions, can be incorporated in a peptide sequence, can be readily synthesised, and have a variety of conformations.

SUMMARY OF THE INVENTION This invention describes novel compounds useful for the synthesis of peptide mirnetics, and describes the use of these compounds WO 99/48913 PCT/AU99/00207 7 for the synthesis of novel reverse turn mimetics. The reverse turn mimetics of the invention have the general structure X, or in a preferred embodiment the general structures 1-VI (which are subsets of the general structure X; see below and Figures 1 and 2 on the attached sheets; the structures are fully described in the detailed description following this summary).

R

2

Q

2 3 4

R

RN M Q RC

M"

x It has now been discovered that B-allyldialkylboranes Rgla-i, Figure 3) react with imines 3 (Scheme 1) to give the novel allyl amines 4a-d in good yield and with a very high degree of chemo- and stereoselectivity. This is surprising because in contrast to these good results, allylation with the related B-allyldialkoxyboranes Rglj, Figure 3) or allylcopper or allylzinc reagents gave inferior results with racemisation and reaction at other functional groups. The reaction of imines 3 to form compounds 4a-d and formation of the related compounds 5-8a-d (all of which are made from compounds 4a-d) forms the basis of the synthesis of all the compounds of the invention, and hence the invention. Thus the allyl amines 4a-d are suprisingly valuable intermediates for the synthesis of new peptide mimetics, particularly reverse turn mimetics, enabling the synthesis of the significant variety of new reverse turn mimetics of the invention (having the general structure by the variety of different pathways described herein. All the mimetic systems of the invention can be incorporated into peptide sequences (i.e.

they include amino and carboxy termini in addition to the sidechain WO 99/48913 PCT/AU99/00207 8 functions), or if desired the amino and/or carboxy termini can be omitted from the mimetic.

As described above, there is a need for a wide range of different mimetics to better reproduce the wide range of conformations found in native reverse turns. The turn mimetics of the invention have a large variety of novel functionalised ring structures, each of these therefore having novel conformational characteristics. Furthermore, the structure and ring sizes of many of the turn mimetics make them well suited to the reproduction of the geometry of the more common native reverse turn conformations (those having d of 4.5A to 6A).

The synthetic methods described in this invention are generally superior to the prior art in terms of the capacity to include a wide range of side chain functions, in all the sidechain positions, without significant changes in the synthetic method; that is, the methods are more truly generic. In addition, the control of chirality in the synthesis of the mimetics of the invention is superior to the prior art an important consideration in the elucidation of structure-activity relationships and the development of novel pharmaceuticals, and other commercially useful peptide mimetics, as diastereomeric mixtures are normally unsuitable and may be impractical or impossible to separate on a commercial basis.

Furthermore, selective access to a range of different diastereomers for a particular mimetic with a given sequence provides a selection of different conformations. Thus in a mimetic with four chiral centres there are a total of 16 (24) possible diastereomers each having a different conformation.

The methods of the invention allow for a high level of chiral control by using available chiral starting materials, non-racemising conditions and diastereoselective reactions.

The invention includes all novel intermediates used in the preparation of the turn mimetics and more generally useful for the preparation of peptide mimetics, particularly Scheme 1 and Scheme 2. Also 11-12, Scheme 3; 13-14, Scheme 4; 16-17, Scheme 18-19, Scheme 6; 21-22, Scheme 7; 23(a-d)-25(a-d), 26, Scheme 8; 27- WO 99/48913 PCT/A U99/00207 9 28, Scheme 11; 29-34, Scheme 12; 35(a-c), 36-38, Scheme 13; 43-46, Scheme DETAILED DESCRIPTION OF THE INVENTION The peptide mimetics of this invention have the general structure X, shown below and defined as follows:-

R

2

Q

Q

2 3 RN R RN M' RC

M"

X

wherein R and R 2 and other R groups referred to hereinafter inclusive of R 3

R

4

R

3 and R"n 4 etc. unless otherwise indicated, are amino acid side chain groups, each independently chosen and therefore the same or different (two separate R groups in the same mimetic do not require a different suffix to indicate that they are independently chosen and can be the same or different). The definition of "amino acid side chain group" as used in this document is the same as the definition of "amino acid side chain moiety or derivative" as described in International Publication W097/15577, pages 7-9 (Kahn, incorporated herein by reference. Amino acid side chain groups typically correspond to, but are not limited to, those found in natural amino acids and derivatives and in common unnatural amino acids. Thus for glycine R hydrogen; for alanine R methyl; for phenylalanine R -CH 2 Ph; for homophenylalanine R -CH 2

CH

2 Ph; for valine R -CH(CH 3 2 for leucine R -CH 2

CH(CH

3 2 p-nitrophenylalanine R -CH 2 ((4-NO 2 )Ph); naphthylalanine R -CH 2 -naphthyl etc. Also included are cyclic amino acid sidechains such as for proline, hydroxyproline and homoproline which involve a cyclization to the adjcent backbone nitrogen atom or the equivalent position, but only where this is possible the amine or WO 99/48913 PCT/AU99/00207 equivalent atom is not already substituted as part of the heterocyclic mimetic framework).

Z is normally hydrogen, methyl, ethyl, formyl or acetyl, and may alternatively be R or -CH 2 R or -C(O)R where R is an amino acid side chain group, or alternatively Z is part of a cyclic amino acid side chain group joined to R 2 (for example to mimic a proline residue at position For II(i) referred to hereinafter, Z cannot be hydrogen due to compound instability.

Rc is the carboxy terminal part of the mimetic, typically C(O)Pgc or alternatively hydrogen or an amino acid side chain group R or

-CH

2

R.

PgC (and Pg c etc.) is a protecting group for carboxylic acid.

typically including, but not limited to: alkoxy, benzyloxy, allyloxy, fluorenyl methyloxy, amines forming easily removable amides, or alternatively an appropriate cleavable linker to a solid phase support, or such a support itself, or alternatively hydroxy -OR, -NHR or remaining C-terminal portion of the mimetic system as described below.

RN is the amino terminal part of the mimetic, i.e. -N(Z')PgN, Z' is normally hydrogen, alternatively methyl (to mimic an Nmethyl amino acid residue at position or alternatively part of a cyclic amino acid side chain group joined to R 1 (for example, to mimic a proline residue at position PgN (and PgN') is a protecting group for amine, typically including, but not limited to: Boc, Cbz, Fmoc, Alloc, trityl; or alternatively an appropriate cleavable linker to a solid phase support, or such a support itself, or alternatively hydrogen or R or -C(O)R where R is an amino acid side chain group, or alternatively part or all of the remaining N-terminal portion of the mimetic system, as described below.

M" are normally hydrogen, alternatively one or more may be C 1

-C

4 alkyl (preferred methyl), chloro, C 1

-C

4 alkoxy (preferred methoxy).

WO 99/48913 PCT/AU99/00207 11 Q1 R 1 and Q2 Z; alternatively there is a cyclisation from Q1 to Q 2 and then in preferred embodiments of the invention Q1Q 2 CH(R)C(O) or -CH 2 CH(R)C(O)- or -CH 2

CH

2

Q

1

Q

2 can also be: -CH(R)CH 2 or -CH 2

CH(R)CH

2 or -CH 2

CH

2

CH(R)CH

2 or

CH

2 CH(R)- or -CH 2

CH

2 CH(R)- or -CH(R)CH 2

CH

2 or

CH

2

CH(R)CH

2

CH

2 or -CH(R)CH 2 or -CH 2

CH(R)CH

2 hydrogen, C1-C4 alkyl, chloro or Cl-C4 alkoxy and Q 3 Y or -C(O)NHCH(R)Y- or -C(O)ENHCH(R)Y-; or alternatively when Q 3

C(O)N(Q

5 )CH(R)Y- Q 5 is a covalent bond from the Q 4 group to the nitrogen atom in Q 3 (a cyclisation forming a bicyclic ring system).

Y is selected from the group consisting of C(0) and CH 2 and

Q

4 is selected from the group consisting of CHM', CH(Q 5

)CH

2 and

CH(Q

5 with the provisos that:

Q

4 Y is C(0); (ii) Q 4 Y is CH 2 (iii) Q 4 CH(Qs)CH 2 Y is and (iv) Q 4 CH(QS)C(0), Y is CH 2 where n 1, 2, 3, (n 1 to about 300, but more typically n is between 1 and 30) and AA is an amino acid residue AA -NHCH(CH 3 for alanine); E is therefore a loop of n amino acids which are linked in a cycle by the rest of the mimetic system.

The loop may also incorporate non-alpha amino acids, alpha dialkyl amino acids or any other amino acid which confers favourable properties on the mimetic system, for example increased binding affinity, or ease of detection, identification or purification. The invention, when used with such larger loops, is functioning as a covalent hydrogen bond mimic (another aspect of the invention), as generally described by Arrhenius et al. (Arrhenius et al., 1987) and also in U.S. Patent 5807979 (Arrhenius et al.).

WO 99/48913 PCT/AU99/0007 12 Preferred embodiments of the invention are the structures I- VI, as ilustrated in Figures 1 and 2 and defined in Table 1:- Table 1 Mimetic Q1 Q2 Q 3 I R 1 Z Y- II R 1 Z -C(O)NHCH(R)Y- M III R 1 Z M

C(O)NHCH(R)C(O)-

NHCH(R)Y-

IV R 1 Z -C(O)N(Q 5 )CH(R)Y- Q 3 V -CH(R)C(O)Q 2 Q1 Y M VI -CH 2

CH(R)C(O)Q

2

Q

1 Y M Recursive entries of Q groups in Table 2 indicate a cyclisation thus mimetics V and VI have a cyclisation between Q 1 and

Q

2 and mimetic IV has a cyclisation between Q 3 and Q 5 In the Tables, the groups Q 1

-Q

5 and Y are as defined above, and the other groups are asdefined herein.

The compounds of this invention have been designed to allow for incorporation in a peptide or protein chain, or for covalent attachment to any molecule or group that may be useful for the enhancement of the biological activity, or other property, of the peptide mimetic. Thus the mimetics typically contain amino and carboxy termini independent of the sidechain functions. The term "remaining C- (or N-) WO 99/48913 PCT/AU99/00207 13 terminal portion of the mimetic" is any group, molecule, linker, support, peptide, protein, nucleoside, glycoside or combination of these, covalently linked to the mimetic. Typically such remaining portions would be peptides or combinations of peptides and other mimetics, or compounds to facilitate detection or identification, or to improve the pharmacodynamics or other useful feature of the mimetic system.

In addition, any R group (an amino acid side chain group) may serve as an attachment point to a solid support, or to a linker to a solid support, or as a covalent attachment point for another molecule that may be useful for the enhancement of the biological activity, or other property, of the mimetic, as described above for the remaining C- or Nterminal portions of the mimetic.

The term "cleavable linker" and "solid phase support" are as defined in International Publication W097/1557 The use of a wavy line for one of the bonds at a chiral centre in the general structures X and I-V and in the other structures in the Figures and Schemes indicates that the centre may be in either the or configuration, or be a mixture in any proportion of the and (S) configurations. In most circumstances it is preferable to avoid mixtures of configurations unless the intention is to provide a mixture of diastereomers for example for the purpose of more efficient screening (by the use of a mixture) or for synthetic expediency. Chirality at the amino acid side chain positions in the compounds of the invention at R 1 to

R

4 is controlled by the use of chiral starting materials (L or D amino acids) and the avoidance of synthetic conditions which cause racemisation. The configuration at chiral centres formed in the mimetic synthesis is dependent on several factors and can be controlled in several cases, but in other cases mixtures of diastereomers will result, which can potentially be separated by physical means. A significant advantage of the invention is the superior level of chiral control possible at the chiral centres in the mimetics.

WO 99/48913 PCT/AU99/00207 14 EXAMPLES OF PREFERRED EMBODIMENTS OF THE MIMETICS fl-Turn mimetics I(i)a, I(ii)a Z and Z' hydrogen): R 1 pgNH HN 2 N H 0 0 I(ii)a pgC fl-Turn mimetics 11(i)a, 1l(iii)a MW and Z' hydrogen, Z Me): MeN H

NN

N H 0 pgN pgC II(i)a ieN. H 0' Pgc pgN II(iii)a fl-Bulge mimetics I1l(i)a, 1II(iii)a M" and Z' =hydrogen, Z Me): R 3 R 3 NH 04 HN_ g 2 H N R M2e N HMe R4R

N

N N R N H NH P 1 0 P gN Pg c Pg N 111(i)a 111(iii)a WO 99/48913 PCT/AU99/00207 Bicyclic El-turn mimetics IV(i)a, IV(ii)a Z and Z' hydrogen): 0

R

H9N IV(i)a

HNA

N H 0 pgC pg

N

IV(ii)a Bicyclic El-turn mimetics V(i)a, VI(i)a, V(ii)a, VI(ii)a MWand M"= hydrogen) 0 R2 N N

R

4 R N\pgN 0 :pgC V(iQa

N

N R 4 R_,pgN 0 PgC VI(i)a

R

N

NN 4 R1 g N N 0Q VI(ii)a 11 ~PgN 0O0 PgC V(ii)a The synthesis of all the mimetics described in this specification may proceed initially by the same general synthetic procedure for formation of the common intermediates reaction of imines 3 with allyl metal reagents Rgl (allyl boranes preferred) to give the allyl diamines 4, which are new, as described in Scheme 1. The other compounds of Scheme 1 5-8) may all be derived from the allyl diamines 4, as described in Scheme 1 and in the comments below. The WO 99/48913 PCT/AU99/00207 16 allylation reaction of imines 3, which falls within the scope of the invention, is remarkable for its mildness and selectivity allowing a wide range of functional groups to be present in the rest of the molecule, a very important consideration in the synthesis of peptide mimetics. Another important feature of the reaction of allylboranes with the imines 3 is that it proceeds in good yield >50% isolated yield) in the sterically hindered general case where R 1 and R 2 are both not hydrogen i.e. for all mimetics of dipeptides not containing glycine. Scheme 1 and all subsequent Schemes describe the preferred case of RN=NHPgN and RC=C(O)Pg c (Figures 1 and analogous methods apply in the general case.

In relation to Scheme 1, preparation of the imines 3 is completed by condensation of an amino acid aldehyde (compound 1) with an amine The aldehydes 1 may be prepared by either oxidative procedures from the corresponding N-protected amino alcohol, or reduction of an N-protected amino acid derivative (Fehrentz and Castro, 1983), the different approaches have been reviewed, (Jurczak and Golebiowski, 1989) (see also Goel et al., 1988, Org. Syn. 67 69). The amines 2a are amino acid esters (or other acid protected amino acid derivatives), which are commercially available or may be synthesised by standard procedures from amino acids. Amines 2b-2d are prepared by reductive amination of an amine 2a and an amino acid aldehyde 1: WO 99/48913 PCT/AU99/00207 17 O R R 0 R H H 2 pgC reductive amination H HH pgC N'O pgN R pgN 1 2a protect amine Pg deprotect Pg N O R Pg

R

P R S0 H2 N CO 2 H N N APgC HH H 2 N pgC

H

R" R 1. couple R 2c 2. deprotect Pg N 2b Amines 2d are prepared by repeated coupling/deprotection steps (as in conversion of 2b to 2c), standard techniques of peptide synthesis.

The reductive amination procedure for the alkylation of amines by aldehydes is well established in the art. (See for example, Sasaki and Coy, 1987, Peptides 8 119), the preferred reagents are sodium cyanoborohydride (Borch et al., 1971; Hutchins and Natale, 1979; Gribble and Nutatits, 1985), or more preferred sodium triacetoxyborohydride in dichloroethane. (Abdel-Magid et al., 1996).

Methods for the formation of amide bonds (coupling) are well established in the art. For coupling at more hindered amines the use of certain reagents, for example those based on 1-hydroxy-7azabenzotriazole (Ehrlich et al., 1993; Carpino et al., 1994), or the use of amino acid fluorides (Carpino et al., 1990; Wenschuh et al., 1994) is advantageous.

Protecting strategies for the synthesis of peptides and peptide mimetics are well established in the art, for example a five dimensional orthogonal strategy was used by Hirschmann and co-workers in the synthesis of a somatostatin mimetic.(Hirschmann et al., 1996) A more general reference work on protection/deprotection is the monograph by Greene and Wuts.(Greene and Wuts, 1991).

WO 99/48913 PCT/AU99/00207 18 The example syntheses described in this document use solution phase chemistry. The mimetics may also be synthesised by analogous solid phase techniques, or by a combination of solid phase and solution phase techniques, or the mimetics may be incorporated in normal solid phase peptide synthesis in the same way as a standard protected amino acid derivative. A review by Fruchtel and Jung (Fruchtel and Jung, 1996) details the state of the art in solid phase organic synthesis (in 1996).

It will be clear to those skilled in the art that the mimetics of the invention, due to their generic methods of synthesis, are suited to the application combinatorial chemistry techniques (more specifically combinatorial organic synthesis) and certain associated identification and screening techniques. The application of combinatorial and associated technologies to drug discovery are well known in the art and have been reviewed, see for example papers by Gallop et al. and by Gordon et al., and references therein, incorporated herein by reference (Gallop et al., 1994; Gordon et al., 1994). Additionally, reference may be made to a review by Thompson and Ellman on the synthesis and application of small molecule libraries, and references therein, incorporated herein by reference.(Thompson and Ellman, 1996).

The imines 3 form rapidly at room temperature on mixing of the amine and aldehyde in an appropriate solvent, e.g. CH 2

CI

2 or diethyl ether, with liberation of water. The water is removed with a drying agent, e.g. dried MgSO 4 which is subsequently removed by filtration. The imines are then reacted with an allyl metal reagent (Rgl) to give, after work-up, compounds 4 (Scheme 1).

In relation to reagents Rgl: standard allyl organometals, such as allyl magnesium bromide, are unsuitable for reaction with imines 3 due to a lack of selectivity for the imine function over the carboxylic acid derived groups (esters, amides) also present in 3. Allyl copper and zinc reagents have been used in selective reactions with imines (Bocoum et al., 1991; Basile et al., 1994) but in the case of imines 3 these reagents WO 99/48913 PCT/AU99/00207 19 result in extensive racemisation at the D-imine chiral centre, and attack esters present in the imine to a significant extent. While some of the desired target 4 may be produced by many allyl metal reagents on reaction with 3, the reaction product typically contains a mixture of four diastereomers and also by-products from reaction at the carboxylic acid derived groups (especially esters). In contrast to these results, reaction of the imines 3 with allyl boranes, such as B-allyl-9borabicyclo[3.3.1]nonane (allyl-9-BBN), Rgla, gives excellent results and reasonable diastereoselectivity isolated yield based on crude aldehyde, and ~80:20 diastereoselectivity where R 1 is not H).

R

2

R

2

R

2

'CO

2 Bn HN C0 2 Bn HN 1CO2Bn R R R SR2B AcOH, NHBoc THF, A NHBoc NHBoc 3a Rgla 4a 4a (R) S) configuration new chiral centre new chiral centre (R) minor product major product By the use of allyl trialkylboranes with appropriate chiral alkyl groups such as B-allyl-diisopinocampheylborane (allyl-DIP, Rglb and Rglc), or the diisocaranylboranes Rgld-e it is possible to produce give only the major product (one diastereomer, >99:1) in good yield and purity. The configuration at the new stereocentre was determined to be when using aldehyde derived from natural configuration amino acids, and the stereocontrol exerted by the O-aldehyde chiral centre was dominant over the effect of chiral boron ligands and over the effect of the other amino acid chirality in all cases examined. The (+)DIP reagent Rglb gave higher diastereoselectivity on imines derived from natural (S) configuration aldehydes than Rg1c (from The purity of the allylation products 4a may also be improved by the removal of the ester protecting group PgC to give a crystalline amino acid which can be recrystallised from ethanol/water) to the desired level of purity and then reprotected.

WO 99/48913 PCT/AU99/00207

R

2

R

2 HN C0 2 R deprotect R +H 2 .N 1CO NHPgN re-protect acid NHPgN 4a 4a Pg C=H crystalline derivative The use of crotyl (Rglf, Rglh-i), methallyl (Rg1g) or other substituted allyl derivatives leads to bridge substituted mimetics (mimetics where at least one of M, M' and M" is not hydrogen) with further opportunities for stereocontrol. The less reactive allyl boronate allyldimethoxyboron (Rglj) was found to give inferior results (significant epimerisation at CD(i)) compared to the allyltrialkylboranes. Many allylboronate and related reagents Rglk-m) are described in the literature, and some of these may be more effective than allyldimethoxyboron for the conversion of 3 to 4. Selective reactions using allylic metals have been reviewed by Yamamoto and Asao, Tables IV and V in the review (pp 2224-2230) list a wide variety of allyl boron reagents.(Yamamoto and Asao, 1993) The preparation of allyl-9-BBN and other allyltrialkylboranes has been described by Brown and coworkers (Kramer and Brown, 1977; Brown and Jadhav, 1983; Brown and Jadhav, 1984; Brown and Bhat, 1986; Brown, Randad et al., 1990) Allyltrialkylboranes may also be prepared by the reaction of the corresponding B-chloro or B-methoxy derivative with an allylmagnesium bromide diethyl ether), and reacted in situ with the imine (Yamamoto and Asao, 1993). The imines 3 formed from two non-glycine derivatives R 1 and R 2 not H) are significantly hindered about the imine nitrogen, and the use of bulky boron ligands (such as diisopinocampheyl) can reduce the reaction yield. For high yield and selectivity smaller chiral B-allyl compounds, e.g. those based on dimethylboracyclopentane are preferred Rgln, Figure 3).

WO 99/48913 PCT/AU99/00207 21 In relation to protection and deprotection of compounds 4 and 5: addition of formaldehyde solution to 4 results in the rapid formation of imidazolidines 5; the relative configuration in the major allylation products 4 results in a 4,5-cis-substituted imidazolidine 5. This protection strategy is important for further reaction of these compounds. The protecting group is removed by treatment with aqueous acid (e.g.

aqueous methanolic acetic acid).

R

2

R

2 S CH20, EtOAc HN CO 2 R (fast) N CO 2

R

NHPgN HOAc, H 2 0, CH 3

OH

(slow) 4a 5a A similar protection system is the dibenzyltriazone group of Knapp and co-workers,(Knapp et al., 1992) the paper describes other deprotection conditions and is incorporated herein by reference. An alternative deprotection method involves the hydrogenation of the imidazolidine system to an amine N-methyl group (40psi H 2 Pd-C, MeOH, 48hrs), a conversion that can be used to give mimetics where Z Me.

In relation to oxidation of alkenes 5: acids 6 can be synthesised directly by oxidative cleavage of the alkenes 5, e.g. by RuCI 3 /NalO 4 aldehydes/ketones 8 may be synthesised directly from 5 by ozonolysis (for oxidation methods see for example the monograph by Hudlicky (Hudlicky) and references therein), but this process is not sufficiently selective and results in over-oxidation and the formation of other by-products. Preferred is the two step process of dihydroxylation (Os0 4 N-methylmorpholine-N-oxide (NMO),tBuOH/water) (VanRheenen et al., 1976; Ray and Matteson, 1980) to 7 followed by oxidative cleavage (Pb(OAc) 4 in benzene or H 5 10 6 in THF).(Hudlicky, 1990) Examination of the products of the oxidation reactions led to the surprising discovery that cleavage with (Pb(OAc) 4 resulted in isomerised product with the substituents now trans, not cis as in the starting material. It was further WO 99/48913 PCT/AU99/00207 22 discovered that oxidation of the diol with H 5 10 6 in dry THF resulted in retention of the 4,5-cis configuration in the aldehyde product 8. The cis aldehydes can also be isomerised to the trans by treatment with catalytic acid, e.g. HCI in CHCI 3

R

2

R

2

R

2 SCo 2 PC Pb(OAc), N co 2 PgC H 5 0 6 -N CO 2 PgC P O benzene TN OHF N pgN Pg N -OH pgN pgN N 0 p gN H p g 8a 4,5-trans, 7a 4,5-cis 8a inversion retention These important discoveries now allow selective access to all of the eight possible diastereomers of the aldehydes 8 and the acids 6, and therefore control of the majority of the chirality in all the mimetic systems described in the invention.

In relation to the oxidation of aldehydes 8 to acids 6: many oxidation reagents may effect this conversion, e.g. pyridinium dichromate.(Hudlicky, 1990) Glycols 7 may also be oxidised directly to acids, e.g. by RuCl 3 /NalO 4 In relation to reduction of acids 6 to aldehydes 8: carboxylic acids 6 can be converted to aldehydes by the same general methods used for the formation of protected D-amino aldehydes described above.(Jurczak and Golebiowski, 1989). The carboxylic acid can be selectively reduced to the alcohol in the presence of carboxylic esters by the use of borane (Brown and Krishnamurthy, 1979), then oxidised to the aldehyde as previously described.(Jurczak and Golebiowski, 1989) In relation to Scheme 2: Aldehydes/ketones 8 undergo reductive amination with amino esters 9 by the methods previously described. The preferred method is NaBH(OAc) 3 in dichloroethane (room temperature). Surprisingly, it was discovered that the reductive amination of 4,5-cis imidazolidine aldehydes 8 resulted in the formation of the WO 99/48913 PCT/AU99/00207 23 trans amines 10 trans:cis). This isomerisation reaction is rapid (much faster than that of aldehydes 8) as the reductive amination reaction is complete in only a few minutes. It was further discovered that the isomerisation reaction could be prevented by the pre-formation of the imine between the aldehyde 8 and amine 9 (in MeOH, 2-4 h at room temperature) with rigorous exclusion of acid, followed by reduction with sodium borohydride to give the cis amine 10 from the cis aldehyde. This discovery allows the selective synthesis of either the 4,5-cis diastereomer or 4,5-trans (9:1 with cis) diastereomer of the amines 10 starting from the 4,5-cis aldehyde 8.

R

2

R

3

R

2 K G H 2 N J Y/NG R 3 PgN-NH 9 P9N-N N 9

C'

1 HOAc, (CH 2

CI)

2 RI H O R NaBH(OAc) 3 8 (4R, 5S) 10 (4S, major product is 4,5 trans inversion R 3 J[P gC

R

2

H

2 N p C', 9 0 1. MeOH, 4h G R 3 2. NaBH 4 PgN-N' ~N p N ff Fi H 0 (4R, product remains 4,5-cis retention It is important to appreciate that the methods described above allow the selective synthesis of all sixteen relative and absolute diastereomers of compounds 8 and 6, and all thirty two diastereomers of compounds 10. The ability to selectively synthesise these diastereomers is a significant advantage of the invention.

In relation to Scheme 3: Deprotection of 10 is by standard methods consistent with the overall protecting strategy, as previously discussed. Many coupling agents are suitable for effecting the cyclisation WO 99/48913 PCT/AU99/00207 24 of 11 to 12, typical conditions: THF, BOP or HBTU or HATU, EtN(i-Pr) 2 (DIEA). The imidazolidine group is then deprotected (as previously described) by hydrogenation (MeOH, H 2 -Pd/C) when Z Me, and by hydrolysis H 2 0) for Z H (other Z groups may be introduced by acylation or alkylation of the deprotected secondary amine).

In relation to Scheme 4: Deprotection and cyclisation of 6b to 13, 14 and I(ii): standard deprotection and coupling (cyclisation) methods are used. Other conversions are as previously described.

In relation to Scheme 5: As previously discussed, coupling reactions to relatively hindered (usually secondary) amines often require the use of specialised coupling conditions such as acid fluorides 15, as described by Carpino et al. (Carpino et al., 1990; Wenschuh et al., 1994) Protecting groups PgN' and PgC' (in 16) are typically benzyloxycarbonyl (Cbz) and benzyl ester, simultaneously deprotected by hydrogenation (0.1M HCI in EtOH, H 2 cyclised using the BOP coupling reagent in THF or DMF, followed by conversion (deprotection) of the imidazolidine group to N-Me by hydrogenation as previously described.

In relation to Scheme 6: Standard deprotection/ coupling conditions as previously described.

In relation to Scheme 7: Where R 4 is a E-branched amino acid side chain (such as in Valine) then the coupling of 6a and 20 may require the use of HATU or other system suitable for a hindered coupling when bulky sidechain groups are present, as previously discussed.

Conditions and protecting groups for the conversion of 21 to 19 are the same as for the conversion of 16 to 11(i), Scheme In relation to Scheme 8: Hydroboration of alkenes is well known in the art, see for example monographs by Brown (Brown, 1975; Pelter et al., 1988) The resulting alkyl boranes can be oxidised to alcohols (using alkaline hydrogen peroxide, or in a preferred embodiment using trimethylamine oxide, or other amine oxide, to form the borate with subsequent liberation of the alcohol by transesterification) (Soderquist and Najafi, 1986). Alternatively, treatment of the borane with acid WO 99/48913 PCT/AU99/0007 dichromate or, in a preferred embodiment, with pyridinium chlorochromate (PCC) gives the aldehyde (Brown et al., 1980; Brown et al., 1986). The aldehydes so formed may be reductively aminated on to amines 9 by the methods previously described.

In relation to Schemes 9-11: Standard synthetic techniques, previously described.

Methods for the synthesis of beta bulge III(i-iv)) and higher loop mimetics follow the corresponding methods for the synthesis of beta turn mimetics II(i-iv). Appropriate protecting groups are chosen so that extra residues can be added to the system prior to cyclisation, as illustrated in Scheme 11 for the synthesis of a ll(i) mimetic.

In relation to Scheme 12: Conversion of 1,2-diols 7 to epoxides 29 (dehydration) may be achieved with a number of reagents, for example triphenylphosphine and a dialkylazodicarboxylate (the Mitsunobu reagents) (Carlock and Mack, 1978; Robinson, Barry et al., 1983) or TsCI/NaOH/PhCH 2 NEt3 CI-.(Szeja 1985). The epoxides 29 alkylate amines 9 on warming in ethanol or DMSO solution to give the amino alcohols 30. The alcohol may then be oxidised to the ketone 32 by the use of TPAP (tetrapropylammonium perruthenate) with Nmethylmorpholine-N-oxide in CH 2

CI

2 /acetonitrile by the method of Griffith and Ley (Griffith and Ley ,1990). For 32 typically PgN'=Cbz and PgC'=Obenzyl, then by the use of catalytic hydrogenation conditions (EtOH, H 2 Pd/C) the protecting groups are both removed and intramolecular reductive amination of the free amine to the ketone occurs to give 33.

Coupling using the BOP reagent (or other suitable conditions) followed by deprotection of the imidazolidine group as previously described gives the bicyclic mimetic IV(i). Alternative syntheses are possible with the use of mild oxidising reagents to convert the glycols to carbonyl compounds, followed by reductive amination (Frigerio and Sangostino, 1994).

In relation to Scheme 13: 1,2 diols can be oxidised without carbon-carbon bond cleavage by the use of certain mild reagents e.g. IBX WO 99/48913 PCT/AU99/00207 26 (Frigerio and Sangostino, 1994). Conversion of 35c to 36 proceeds by intramolecular reductive amination, or alternatively 35a can be reductively aminated onto 2b, as indicated. Reductive amination, coupling and deprotection details are as previously described.

The syntheses for the bicyclic O-turn mimetic systems V and VI are accomplished from the corresponding D-turn mimetic systems I, where the R 1 side chain group is derived from an aspartic acid or glutamic acid (VI) derivative.

O R3 R 3

R

R 2 N Y 4 N

R

4 R XNR X M' RC R'N RC PgN M" p MM V VI X Y X Y V(i) CH(M) C(O) Vl(i) CH(M) C(O) V(ii) C(O) CH 2 Vl(ii) C(O) CH 2 The synthesis of mimetics V and VI thus proceeds as in Scheme 1, with the aldehyde component 1 (Scheme 1) being of the form Id or le (Scheme 14), with the R and Pg groups as previously defined.

The synthesis follows the synthesis of D-turn mimetic systems I, and is completed by the method illustrated in Scheme In relation to the preparation of alkylated aspartic and glutamic acid derivatives Id and le the alkylated derivatives 39-42 can be prepared by a number of methods known in the art. Selected methods are summarised in Schemes 16 and 17. Rapoport and co-workers have developed methods for the selective alkylation of N-phenylfluorenyi protected aspartic and glutamic acid derivatives (Koskinen and Rapoport, 1989; Wolf and Rapoport, 1989). A review by Sardina and Rapoport, and references contained therein, describe several methods for the synthesis of alkylated aspartic and glutamic acid derivatives, incorporated herein by WO 99/48913 PCT/AU99/00207 27 reference (Sardina and Rapoport. 1996). Derivatives 39-42 are converted to aldehydes Id and le by the methods previously described for for the preparation of aldehydes 1.

The use of standard chemical techniques, in particular the Arndt-Eistert homologation reaction (Meier and Zeller, 1975) and reductions of carboxylic acids to aldehydes (Jurczak and Golebiowski, 1989), and also the synthesis of ketones -C(O)R from amides C(O)N(OMe)Me (Nahm and Weinreb, 1981), to modify the aspartic and glutamic acid or their alkylated derivatives, or the use of similar derivatives of non-natural amino acids, such as homo-glutamic acid, enables the synthesis of the other compounds of the invention in which

Q'Q

2 (in the general structure X) forms part of a cyclic system, defined as: -Q 1

Q

2

-CH

2

CH

2 CH(R)C(O)- (from sidechain alkylated homoglutamic acid); -CH(R)CH 2 (from aspartic acid by reduction of the O-carboxylate and reductive amination); -CH 2

CH(R)CH

2 (from glutamic acid by reduction of the O-carboxylate and reductive amination);

CH

2

CH

2

CH(R)CH

2 (similarly from homoglutamic acid); -CH 2

CH(R)-

(from an aspartic acid sidechain ketone -CH 2 C(O)R by reductive amination); -CH 2

CH

2 CH(R)- (from a glutamic acid sidechain ketone

CH

2

CH

2 C(O)R by reductive amination); -CH(R)CH 2 (postalkylation sidechain homologated aspartic acid); -CH 2

CH(R)CH

2

C(O)-

(post-alkylation sidechain homologated glutamic acid); -CH(R)CH 2

CH

2 or -CH 2

CH(R)CH

2

CH

2 (from reductive amination of reduced postalkylation sidechain homologated aspartic acid or glutamic acid derivatives).

In relation to Scheme 18: An alternative procedure for the synthesis of intermediate compounds 10 (or equivalent) can be used in the case where R 1 is hydrogen and M, M' and M" are also hydrogen, as described in Scheme 18. Compound 49 is available commercially with certain N-protecting groups or can be made by coupling N-protected glycine with N,O-dimethylhydroxylamine. Reaction with vinylmagnesium bromide in analogy to the general procedure of Rapoport and co-workers WO 99/48913 PCT/AU99/00207 28 (Cupps et al., 1985; Boutin and Rapoport, 1986) results in formation of the O,D-unsaturated ketone 50. Conjugate addition of an amino acid ester 9 THF) results in the formation of aminoketones 51 which can be N-protected by standard procedures to form ketones 52 before reductive amination of an amino acid ester 9 under the conditions described by Abdel-Magid et al. (Abdel-Magid et al., 1996) (NaBH(OAc) 3 dichloroethane) to form 54. Deprotection to 55 and coupling gives the 0turn mimetics I(i)a (where as indicated. Alternatively the aminoketones 51 can be acylated with an amino acid fluoride 15 to give compounds 53 which can be deprotected and cyclised (by reductive amination) by hydrogenation in mild acid conditions (H 2 /Pd-C, 0.1M HCI in EtOH). The reductive amination-cyclisation is diastereoselective, only one diastereomer of the mimetics I(i)a were formed from 53, with the configuration at the new stereocentre controlled by the R 2 stereocentre.

The configuration at R 2 gives at the new centre. In contrast, the reductive amination to form amines 54 proceeds with lower stereoselectivity with the major diastereomer having the (R) configuration when R 2 is These discoveries provide further opportunity for stereocontrol in the synthesis of the turm mimetics.

Deprotection of compounds 54 and reaction with formalin in THF is an alternative method for synthesis of compounds 10 as described in Scheme 18.

WO 99/48913 WO 9948913PCT/A U99/00207 29 EXAMPLE SYNTHESES Example Synthesis of a [I-turn mimetic I(i) by the general procedure A mimetic for the sequence HTyr-Gly-Gly-Phe, which is found in the enkephalins, was synthesised with a [i-turn mimetic based on the Tyr-Gly-Gly tripeptide. Similar mimetics have shown activity at opiate receptors (Huffman, Callahan et al., 1988; Huffman et al., 1989).

The synthesis is summarised. in the following scheme:- L IA I H,, Boc C Hc 3 Et 2 o 56 R p-(OBn)Ph

R

N -4H Boc o 57 CH 2

CI

2 MgSO4

H

2 ff'Co 2 B n

R

N K 1H BocN) 58 C0 2 Bn Rgl b Boc C0 2 Bn 59 CH 20 -H 2 0 Bn0 2

C

1 -OsO 4

/NMO

2. Pb(OAC) 4,C 6

H

6 Bn0 2

H

2 '0c 2 c H 3 K NaBH(OAc) 3.

0 HOAc, DCE O n62 H Co 2

CH

3 Boc- 64 C2 DIPEA, 0 EtOAc, A Bo 63 Li OH, MeOH

H

2 0 0 2

CH

3 N H 2 CH 2 CH 2Ph, c ou pie HOAc, H 20N MeOH

N

WO 99/48913 PCT/AU99/00207 Preparation of 56: The amide 56 was synthesised from commercially avaliable Boc-Tyrosine(OBn)OH by coupling with N,O-dimethylhydroxylamine hydrochloride, 1 equivalent, in DMF/CH 2

CI

2 using HBTU reagent (1 eq.) and DIEA (2 eq.) at room temperature. The CH 2

CI

2 was evaporated in vacuo and the residue partitioned between diethyl ether and aq.

NaHCO 3 The aqueous layer was separated and the ether layer washed in turn with 1M HCI aq. NaHCO 3 brine, and then dried over MgSO 4 Filtration and removal of the solvent in vacuo left the product amide 56 as a white crystalline solid in >90% yield. Further purification was carried out by silica gel chromatography eluting with ethyl acetate in petroleum ether, or by recrystallisation from ether. 1 H NMR (300 MHz, CDCI 3 7.46-7.28, 5H, m, OBn; 7.08, 2H, d, J=8.5 Hz, Tyr Ar; 6.90, 2H, d, Hz, Tyr Ar; 5.15, bd, J=8 Hz, NH; 5.04, 2H, s, )OCH 2 Ph; 4.91, 1H, bm, PheO; 3.65, 3H, s, OCH 3 3.16, 3H, bs, NCH 3 3.00, 1H, dd, J=6, 13.5 Hz, Phel; 2.83, 1H, dd, J=7, 13.5 Hz, PheD; 1.40, 9H, s, Boc. 13C NMR MHz, CDCI 3 E 172.3; 157.6, Tyr Ar-O; 155.1, carbamate; 137.0, ipso; 130.4; 128.8; 128.5; 127.8; 127.4; 114.7; 79.5, tBoc; 69.89,

OCH

2 Ph; 61.43, TyrO; 51.55, OCH 3 37.89, NCH 3 32.00, TyrO; 28.26, Boc.

Preparation of 57: The aldehyde 57 was prepared by the method of Fehrentz and Castro (Fehrentz and Castro, 1983) as follows: to a stirred solution of 4.2 g of amide 56 in 100 mis of anhydrous diethylether cooled to 0°C was added 0.51 g lithium aluminium hydride. After 10 minutes a solution of NaHS0 4 in 30 mis of water was added. The reaction mixture was diluted with more ether and washed with 1M HCI, saturated aqueous sodium bicarbonate and brine and dried over magnesium sulphate. The volatiles were removed under reduced pressure to give a waxy solid which was recrystallised from cold ether/hexane to give 2.6 g of 57 as a white solid. 1 H NMR (300 MHz, CDCI 3 0 9.62, 1H, s, aldehyde; 7.50-7.25, 5H, m, Ar(OBn); 7.10, d, J=8 Hz, Ar(Tyr); 6.93, 2H, d, J=8 Hz, WO 99/48913 PCT/AU99/00207 31 Ar(Tyr); 5.10, 1H, b, NH; 5.05, 2H, s, OCH 2 Ph; 4.39, 1H, q, J=7 Hz; TyrO; 3.06, 2H, d(ABX), J=7 Hz, TyrO; 1.44, 9H, s, Boc. 13 C NMR MHz, CDCI 3 0 199.6; 157.8, TyrOAr; 155.3, carbamate; 136.9, ipso; 130.3; 128.5, 127.9, 127.4: ArCH; 115.0, ArCHTyr; 80.08, tBoc; 69.69,

OCH

2 Ph; 60.82, Tyro; 34.51, Tyro; 28.22, Boc.

Preparation of 58: The imine 58 was formed by the reaction of the aldehyde 57 (1.4 g) with one equivalent of glycine benzyl ester in 10ml CH 2

CI

2 (stir at room temperature 1 h) the water formed was removed with magnesium sulphate which was then removed by filtration.

1 H NMR (300 MHz, CDCI 3 0 7.68, 1H, s, imine; 7.49- 7.30, 10H, Ar; 7.15, 2H, d, J=8 Hz, TyrAr; 6.92, 2H, d, J=8 Hz, TyrAr; 5.67, 1H, bd, J=6 Hz, NH; 5.20, 2H, s, OCH 2 Ph; 5.05, 2H, s, OCH 2 Ph; 4.51, 1H, bm, TyrO; (4.26, 4.22), 2H, AB, J=15.5 Hz, GlyO; 3.15, 1H, bdd, J=5.0, 13.5 Hz, Tyrb; 2.93, 1H, dd, J=8.0, 13.5 Hz, Tyrb; 1.48, 9H, s, Boc. 13 C NMR (75 MHz, CDCI 3 0 169.3; 167.4, CH imine; 157.5; 155.1; 136.9, 135.3: 2x ipso; 130.4, CHAr; 128.8, Tyr ipso; 128.44, 128.39, 128.26, 128.19, 127.76, 127.29, 114.65: ArCH; 79.22, tBoc; 69.81, TyrOCH 2 Ph; 66.60, GlyOCH 2 Ph; 60.48, TyrO; 54.73, GlyO; 37.97, Tyr0; 28.23, Boc.

Preparation of 59: A 0.5 molar solution of allyl borane reagent dlpc 2 Ballyl (Rglb) was prepared by the addition of allylmagnesium bromide to one equivalent of (+)DIP-CI in anhydrous diethyl ether under dry nitrogen.

Brown and Jadhav, 1983). The solution of imine 58 in CH 2

CI

2 was stirred and cooled to -78°C under dry nitrogen and one equivalent of the previously prepared dlpc 2 Ballyl solution added. The mixture was allowed to warm gradually to room temperature (overnight). The volatiles were removed under reduced pressure and the residue dissolved in THF and 1 ml of glacial acetic acid added. The mixture was refluxed overnight and then the volatiles removed under reduced pressure. The crude product was dissolved in CH 2

CI

2 petroleum ether and the precipitate filtered off.

WO 99/48913 PCT/AU99/00207 32 The residual oil was chromatographed on flash silica eluting with ethyl acetate petroleum ether to give 1.3 g (60% yield based on 57) of 59.

TLC 1:2 EtOAc:light pet. Rf=0.40. 1 H NMR (300 MHz, CDCl3): 0 7.48- 7.30; 10H, Ar; 7.13, 2H, d, J=8.5 Hz, TyrAr; 6.91, 2H, d, J=8.5 Hz, TyrAr; 5.84, 1H, m, vinyl CH; 5.17, 2H, s, TyrOCH 2 Ph; 5.16, 2H, m, vinyl CH 2 5.05, 2H, s, GlyOCH 2 Ph; 4.90, 1H, bd, J=8.5 Hz, NHBoc; 3.95, 1H, bm, TyrO; 3.54, 2H, s, GlyO; 3.82, 1H, dd, J=4.5, 14.4 Hz, TyrO; 2.73, 3H, be; NH(amine), TyrO, CH(homoallyl); 2.28, 2H, m, allyl; 1.35, 9H, Boc.

13C NMR (75 MHz, CDCl3): E 172.1; 157.3; 155.6; 137.1, 135.4: ipso; 134.9, CHvinyl; 130.6, ipsoTyr; 130.0, 128.5, 128.4, 128.3, 127.8, 127.3: ArCH; 117.8, CH 2 vinyl; 114.7, TyrArCH; 79.05, tBoc; 69.90, TyrOCH 2 Ph; 66.51, GlyOCH 2 Ph; 59.38, TyrO; 53.46, CH; 49.28, GlyD; 35.44: coincident allyl carbon and TyrO; 28.20, Boc. Mass Spectrum (ISMS) m/z 545.1 (MH calculated for C 3 2

H

45

N

3 0 5 544.

Preparation of The amine 59 (930 mg, 1.7 mmol) was dissolved in ethyl acetate (15 mL) and 37% aq. formaldehyde solution added (1 mL). The solution was stirred vigorously at room temperature for 1 h (or until the reaction was complete) and then diluted with ether (100 mL) and washed in turn with aq. NaHCO 3 water brine and then dried (MgSO 4 Removal of solvent in vacuo left an approximately quantitative yield (950 mg) of the crude product 60 which was used in the next reaction or further purified by flash chromatography eluting with 10-15% ethyl acetate in light petroleum. TLC 33%EtOAc:light pet. Rf=0.56. The NMR spectra were quite broad in CDCI 3 amide rotamers were present in the approximate ratio 2:1. 1 H NMR (300 MHz, CDCI 3 0 7.50-7.27, 10H, m's, Ar; 7.09, 2H, m, Ar; 6.90, 2H, d, J=8.5 Hz, Ar; 5.64, 1H, bm, vinyl CH; 5.19, 2H, s,

OCH

2 Bn; 2H, m, vinyl CH 2 5.05, 2H, s, OCH 2 Bn; 4.59. 1H, bm, ring NCH 2 4.17, 1H, bm, ring NCH 2 4.06, 1H, bm, TyrD; 3.70, 1H, d, J=17 Hz, GlyO(a); 3.42, 1H, bd, J=17 Hz, GlyO(b); 3.16, 1H, bm, TyrC'H(ring); 2.84, 2H, bm, TyrO; 2.31, 2H, m, allylCH2; 1.38, -3H, bs, Boc minor rotamer; 1.19, -6H, s, Boc major rotamer. 13C NMR (75 MHz, WO 99/48913 PCT/AU99/00207 33 CDC13): D00(peaks due to the carbamate rotamers are placed in parentheses, major rotamer first) 169.8 (ester); 157.2 (tyrosine O-ipso); (153.1, 152.8) carbamate; 137.2 (ipso); 135.4 (ipso); 134.2 (CH vinyl); 131.3 (ipso); 130.5, 128.5, 128.4, 128.3, 127.8, 127.4, 127.3, 126.9: ArCH; 117.5 (vinyl CH 2 114.7 (2xTyrArCH); 79.52 (Boc tertiary); 69.93 (CH 2 66.95 (CH 2 66.46 (CH 2 64.27 (59.65, 58.76) 51.60 (CH 2 34.34 (CH 2 (32.20, 31.93) (CH 2 (27.93, 28.25) (Boc 3xCH 3 Mass Spectrum (ISMS) m/z 557.1 calculated for

C

34

H

40

N

2 05: 556 fragments (OR 60): 501.1, (-tBu).

Preparation of 61: To 220 mg of 60 was added 60 mg of N-methylmorpholine- N-oxide (NMO), 40 mg of a 2.5% (by weight) solution of osmium tetroxide in t-butanol, 4 mis of t-butanol and 0.5 mis water. The mixture was stirred at room temperature until the reaction was complete (about 24 hours). 3 mis of 10% NaHSO 3 was added, the solution stirred for 10 minutes, then neutralised with sodium bicarbonate, diluted with brine and extracted three times with ethyl acetate. The combined extracts were washed with brine and dried over magnesium sulfate. Removal of volatiles under reduced pressure gave the crude diol in good yield as an oil which could be used in the next reaction or purified if required by chromatography on silica gel eluting with ethyl acetate. Mass Spectrum (ISMS) m/z 591.3 calculated for C 34

H

42

N

2 0 7 590.

Oxidation of diol using Pb(OAc) 4 The diol (100 mg, 0.17 mmol) was dissolved in dry benzene (4 mL) and Pb(OAc) 4 (85 mg, moistened with acetic acid) was added. After 10 min stirring at room temperature the reaction was filtered, the solvent removed in vacuo and the residue purified by flash chromatography eluting with 25%EtOAc in light petroleum. Yield of the aldehyde 61 was 32% (30 mg). (No efforts to optimise the yield were made. Yield might be improved, for example, by partitioning the crude reaction mixture between aq.base and EtOAc to ensure none of the amine product was lost on filtration of the insoluble salts.) TLC 50%EtOAc in light pet. Rf=0.51. NMR analysis (NOESY WO 99/48913 PCT/AU99/00207 34 experiment) indicated the 4,5-trans ring conformation the 4(S) isomer). 1H NMR (300 MHz, CDCI3): 0 9.52, 1H, t, J=1.5 Hz, aldehyde; 7.50-7.25, 10H, m, ArH; 6.92, 2H, d, J=9 Hz, TyrAr; 5.15, 2H, s,

OCH

2 Ph; 5.05, 2H, s, OCH 2 Ph; 4.65, 1H, bm, ringCH 2 3.88, 1H, bm, TyrO; 3.80, 1H, bm, ringCH 2 3.45, 1H, d, J=16 Hz, GlyO; 3.44, 1H, m, ringCH(Ealdehyde); 3.28, bd, J=16 Hz, GlyO; 3.17, 1H, bm, TyrO; 2.80, 1H, dd, J=9.0, 13.5 Hz, TyrO; 2.51, 1H, J=6, 17 Hz, Oaldehyde; 2.28, 1H, dd, J=17, 4.5 Hz, Daldehyde; 1.50, 9H, Boc. 13C NMR MHz, CDCI 3 (rotamers): D 200.5; 169.9; 157.5; 153.1; 136.9; 135.3; 130.5, 129.6, 128.6, 128.5, 128.4, 127.6, 127.4, 115.0: Ar; 80.21, tBoc; 69.92, OCH 2 Ph; (67.08, 66.86) br, CH 2 66.58, OCH 2 Ph; (62.93, 62.56) br, CH; (61.35, 60.72) br, CH; 52.14, CH 2 46.36, CH 2 (38.5, 37.27) br,

CH

2 28.38, Boc. Mass Spectrum (ISMS) m/z 559.1 (MH calculated for

C

33

H

38

N

2 0 6 558.

Preparation of 62 and 63: The aldehyde 61 (30 mg, 500mol) was dissolved in 1,2dichloroethane (5 mL) and glycine methyl ester hydrochloride (50 mg) and NaBH(OAc) 3 (50 mg) added. The reaction was stirred at room temperature and was complete in a few minutes (<15 min). The reaction was diluted with ethyl acetate, and washed in turn with aq.NaHCO 3 water, brine and then dried (MgS0 4 Evaporation of the solvent left the crude product 62 as a clear oil: TLC 1:1 EtOAc:light pet. Rf=0.17. Mass Spectrum (ISMS) m/z 632.3 calculated for C 32

H

4 5

N

3 05: 631 Analysis of the product or the reaction mixture after overnight standing revealed the formation of a new product with a mass spectrum corresponding to the target cyclised material 63 (MH =524Da). Thus the amine product 62 was not generally isolated but converted directly to 63.

The spontaneous cyclisation was accelerated by the addition of base (i- Pr 2 NEt). After removal of solvent by evaporation under reduced pressure and the product was purified by flash chromatography eluting with EtOAc in light pet. TLC: 1:1 EtOAc:light pet. Rf=0.51. 1 H NMR (300 MHz, CD 3 CN): 0 7.47-7.29, 5H, m, ArH; 7.12, 2H, m, Tyr; 6.92, WO 99/48913 PCT/AU99/00207 2H, m, Tyr; 5.07, 2H, s, OCH 2 Ph; 4.35, 1H, d, J=5.4 Hz; ABq, Ea=4.05, Eb=4.02, JAB=17.4 Hz; 3.70-3.52, 6H, overlapped signals (includes: 3.65, 3H, s; 3.58, 1H, dd, J=11.2, 15.2 Hz); 3.49-3.32, 2H, br m's; 3.15, 1H, br dd, J=5.5, 15.5 Hz; 2.99, 1H, br dd, J=13.4, 14.9 Hz; 2.80, 1H, vbr m; 2.68, 1H, vbr m; 1.64, 1H, m; 1.46, 10H, s m, Boc resonance obscures multiplet. 13 C NMR (75 MHz, CD 3 CN), rotamers, in approximate ratio 3:2, split some peaks and are recorded in parentheses: 0 173.3; 171.5; 158.8; 155.0, br; 138.9; 132.0; 129.9; 129.2; 129.0: 116.1; 80.84; 71.01; (70.87, 69.99); (68.12, 67.45); (65.47, 64.89); 55.76; 52.93; 51.45; 49.95; (39.00, 37.53); 31.87; 28.97 (Boc). Mass Spectrum (ISMS) m/z 524.3 calculated for C 29

H

37

N

3 0 6 523.

Preparation of compounds 64 to 66: The product 63 was hydrolysed with LiOH/H 2 0/MeOH to the acid 64 (mass spectrum MH+=510) and then coupled

(DMF/CH

2

CI

2 /HBTU/DIEA) with phenethylamine using standard procedures and work-up to give 65. The imidazolidine ring of 65 was deprotected with a solution of acetic acid-methanol-water stirred as a very dilute solution for several days then lyophilised) to give crude 66 as a white amorphous solid. Mass Spectrum (ISMS) m/z 601 calculated for C 3 5

H

44

N

4 0 5 600.

Example Synthesis of a (4,5)-cis imidazolidine aldehyde by oxidation of a diol.

C2O A O H COBn 2 SOH H 5 10 6 THF N C> Boc Bo 67 68 OBn For the preparation of the 4,5-cis aldehyde 68 (in this case the 4(R) isomer) the diol 67 prepared from alkene 60 (as described above) (1mmol) was dissolved in THF (10 mL) and H 5 10 6 (1 mmol) WO 99/48913 PCT/AU99/00207 36 dissolved in THF (-20 mL) was added and the reaction stirred at room temperature. A precipitate of iodic acid rapidly formed and the reaction was complete in <5 min. The THF solution was diluted with ether and washed in turn with 10% aq.Na 2

CO

3 water, brine and then dried (MgSO 4 The product aldehyde 68 was formed in good yield and purity.

Contact with acid should be minimised to prevent isomerisation to the trans aldehyde and/or decomposition, for example avoid chloroform as an NMR solvent unless recently made acid free. Yield was 60-80%. TLC: in light pet. Rf=~0.5. 1 H NMR (300 MHz, CD 3 CN): (peaks moderately broad; the Boc rotamers were not resolved although the Boc peak was asymmetric and very broad) 9.48, 1H, bm, aldehyde; 7.5-7.3, m, 2xBn; 7.09, 2H, bd, J=7.5 Hz, Tyr Ar; 6.88, d, 8.2 Hz, Tyr Ar; 5.13, s, 2H, OCH 2 Ph; 5.05, s, 2H, OCH 2 Ph; 4.38, 1H, d, 6.0 Hz,

NCH

2 4.22, 1H, m, TyrO; 4.02, 1H, br, NCH 2 3.56, 1H, bd, J=17.2 Hz, GlyO(a); 3.48, 1H, m, TyrC'H; 3.29, 1H, bd, J=17.2 Hz, GlyO(b); 2.57-2.88, 4H, e, TyrO CH 2 and O-aldehyde CH 2 2.22, s, H 2 0; 1.48-1.08 (1.20 peak), 9H, vbr, Boc 3xCH 3 13 C NMR (75 MHz, CD 3

CN):

E 201.9; 171.4; 158.7; 154.3; 139.0; 137.6; 132.6; 131.9, 129.92, 129.85, 129.6, 129.2, 128.9, 116.0: ArCH; 80.41 (Boctert.); 70.99 (CH 2 67.62 (br, CH 2 67.44 (br, CH 2 60.29 (2xCH, co-incident peaks determined by comparative intensity); 52.99 (br, CH 2 43.58 (br, CH 2 35.94 (br, CH 2 28.78 (br, Boc 3xCH 3 Example Synthesis of O-turn mimetics I(i) for the Gly-Phe-Leu sequence by the short method (which can be used when R 1 hydrogen) WO 99/48913 PCT/AU99/00207 BocHN Me f OMe MgBr 69 THF, OIC NHBoc 0 70 CHMe 2

H

2 N CO 2 Me

THF

NHBoc CHMe2 S71 CO 2 Me NHBoc CHMe 2 Cbz-CI Cbz

N

72 CO2Me 0 72 CO 2 Me rPh

H

2 N CO 2 Bn NaBH(OAc) 3 HOAc, DCE NHBoc bz CHMe 2 HN CO 2 Me S CO02Bn Ph- 73 Major product was (R) at new stereocentre ,Ph 1. H 2 /Pd-C 2. BOP, DIPEA Ph Boc NH HN CHMe 2 74

CO

2

CH

3 (major diastereomer) 75 CO 2

CH

3 (minor diastereomer) Preparation of 69: Boc-glycine was coupled with N,O-dimethyl hydroxylamine hydrochloride, 1 equivalent, in DMF/CH 2

CI

2 using HBTU reagent (1 eq.) and DIEA (2 eq.) at room temperature. The CH 2

CI

2 was evaporated in vacuo and the residue partitioned between diethyl ether and aq.

NaHCO 3 The aqueous layer was separated and the ether layer washed in turn with 1M HCI aq. NaHCO 3 brine, and then dried over MgSO 4 Filtration and removal of the solvent in vacuo left the product amide 69 as a viscous oil that slowly crystallised to a waxy solid and was further purified by chromatography on silica gel. Yield was 1 H NMR (300 MHz, CDCl3): 0 5.3, 1H, bs, NH; 4.09, 2H, bd, OH 2 3.72, 3H, s, OCH 3 3.20, 3H, s, NCH 3 1.46, 9H, s, Boc. 13C NMR (75 MHz, CDCI 3 0 79.6; 61.4; 41.7; 32.4; 28.3.

Preparation of A solution of 11.6 g (53 mmol) of Boc-glycine N,Odimethylhydroxylamide in dry THF (70 mL) under nitrogen in a 250 mL round bottom flask was stirred and cooled in an ice bath. To this was added vinyl magnesium bromide in THF (-120 mmol of a 1M solution) by WO 99/48913 PCT/AU99/0007 38 syringe over 10 minutes. The solution was stirred for 2 h and then quenched by pouring into a mixture of crushed ice and 1M HCI which was then extracted with CH 2

CI

2 The organic extracts were washed with water/brine aq. NaHCO 3 and water/brine followed by drying over MgSO 4 Evaporation of the solvent left 9.6 g of a mobile oil (98% crude) which by NMR was ~95% the ketone product 70. This material was used without further purification in the conjugate addition step. 1 H NMR (300 MHz, CDCI 3 0 6.37, 2H, m (ABX, Jab=2.5 Hz, Jax/bx=9.0, 17.5 Hz), vinyl CH 2 5.95, 1H, dd, J=2.5, 9.0 Hz, vinyl CH; 5.37, 1H, bs, NH; 4.26, 2H, d, J=4.6 Hz, glycyl 1H 2 1.46, 9H, s, Boc. 13C NMR (75 MHz,

CDCI

3 D 194.9 ketone; 155.8 carbamate; 133.6 vinyl; 129.6 vinyl; 79.8 tBoc; 48.32 GlyO; 28.28 Boc.

Preparation of 71: To a solution of 3.0 g (-15 mmol) of crude 70 in THF mL) was added 3.4 g of leucine methyl ester hydrochloride eq) and 2.4 g (1.2 eq) of diisopropylethylamine. After 2 h the reaction was diluted with ether (200 mL) and extracted with cold 1M HCI (3x50 mL) (discard this ether layer). The aq. extracts were immediately neutralised with solid NaHCO 3 and this solution was then back extracted with ether, and the ether washed with water (x3) and finally brine and dried over MgSO 4 Evaporation of the solvent left -5.3 g of product 71 as an oil with very good purity, contaminated with a small amount of leucine methyl ester.

Flash chromatography to separate the product was not very successful as the amine and amino ketone tended to co-elute. TLC EAILP Rf=0.35. 1

H

NMR (300 MHz, CDCI 3 o 5.36, 1H, bm, NHBoc; 4.03, 2H, d, J=5 Hz, GlyD; 3.72, 3H, s, OCH 3 3.26, 1H, t, J=7.5 Hz, Leu; 2.93, 1H, dt, J=12, 6 Hz; 2.72, 1H, dt, J=12, 6 Hz; 2.50, 2H, m; 2.0, 1H, bs, NH; 1.69, 1H, m, LeuD; 1.45, 11H, m, Boc(9H) and Leuo(2H); 0.90, 6H, m, LeuO. 13C NMR (75 MHz, CDCI 3 0 205.1; 176.1; 155.5; 79.8 tBoc; 60.04; 51.64; 50.53; 42.63; 42.57; 40.55; 28.26 Boc; 24.81; 22.63; 22.17. Mass Spectrum (ISMS) m/z 331.4 calculated for

C

16

H

30

N

2 0 5 330; fragments (OR 60): 275.2 (-tBu).

WO 99/48913 PCT/AU99/00207 39 Preparation of 72: The amine 71 was protected as the benzyl carbamate by standard procedures as follows: the crude amine product 71 (1.68 g, mmol) was dissolved in ethyl acetate (30 mL) to which was added a solution of KHCO 3 (1.2 g) in water (15 mL). This mixture was vigorously stirred and cooled in an ice bath and to it was added benzyl chloroformate (780 uL of a 95% solution, 5.2 mmol) dropwise over 5 min. The reaction was stirred for a further 15 min then allowed to warm to room temperature with stirring for an additional 2 h. After this time the mixture was diluted with ether (100 mL), the aqueous layer seperated, and the organic layer washed with 1M HCI, aq. NaHCO 3 brine and then dried over MgSO 4 Evaporation of the solvent left -2.6 g crude oil which was purified by flash chromatography eluting with 25%EtOAc in light pet; combination of the main fractions gave a yield of 86% (2.02 g) of 72. TLC EA:2LP Rf=0.56.

NMR signals split due to amide rotamers are placed in parentheses where possible. 'H NMR (300 MHz, CDCl3): 0 7.40-7.23, 5H, Ar; 5.28- 5.02, 3H, m's, CH 2 Ph NH; (4.64, m, 4.43, m) 1H; (3.98, bs, 3.88, bs) 2H; 3.72-3.51, 4H, includes (3.67, s, 3.55, s) OCH 3 1H; 3.45, 1H, m; 2.78, 2H, m; 1.75, 2H, m; 1.53, 1H, m; 1.43, 9H, s, Boc; 0.91, 6H, m, LeuO CH 3 x2. 13 C NMR (75 MHz, CDCI 3 0 (204.9, 204.5) ketone; (172.5, 172.3) ester; (156.1, 155.8) carbamate; 155.6, carbamate; (136.2, 136.0) ipso; 128.5, 128.2, 128.1, 128.0: ArCH; 79.80, tBoc; 67.48; (58.50, 58.32); 52.12; 50.30; (41.37, 39.87, 39.78, 38.87, 38.60, 37.98) 3C; 28.23, Boc; (24.83, 24.67); 23.09; (21.46, 21.39). Mass Spectrum (ISMS) m/z 465.3 calculated for C 24

H

36

N

2 0 7 464; fragments (OR 70): 409.2, 365.2, (-Boc).

Preparation of amines 73: To a solution of 72 (700 mg, 1.5 mmol) in 15 mL of 1,2dichloroethane was added phenylalanine benzyl ester p-toluene sulfonate (900 mg, 2.1 mmol) and sodium triacetoxy borohydride (850 mg, mmol). The mixture was stirred at room temperature for 24 h and then the solvent removed under vacuum and the residue partitioned between ethyl WO 99/48913 PCT/AU99/00207 acetate and aq. NaHCO 3 the aqueous layer separated, and the organic layer washed with water then brine and then dried over MgSO 4 Evaporation of the solvent left 1.2 g crude oil which was purified by flash chromatography eluting with 25-40% EtOAc in light petroleum ether to give a yield of 76% (800 mg) of the product (a clear oil). The product diastereomers 73 were not seperable under these chromatography conditions. TLC 40%EA in LP Rf=0.48. 1 H NMR (300 MHz, CD 3 CN): D (not very informative due to the presence of rotamers/diastereomers) 7.45-7.05 aromatic protons; (5.46 m, 5.31 m)~1/2H; 5.15-5.00, ~4H, m,

OCH

2 Ph; 4.95, -1/4H, m; (4.51, m, 4.37, 1H; 3.85-3.10, -5H, e (including 3.63, s, 3.58, s: 3H, OCH 3 3.10-2.70, 5H, e; 2.45 broad water peak; 1.80-1.45, 5H, m's; 1.40, 9H, s, Boc; 0.90, 6H, bs, LeuS.

13 C NMR (75 MHz, CD 3 CN): o (signals are grouped in parentheses where they can be reasonably assigned to equivalent carbons in the different diastereomers/rotamers) (175.6, 175.4(br)); 173.6; 157.4, 157.2 (139.0, 139.2, 138.5, 138.3, 137.3) 3x ipso; 130.8, 130.7 129.9 129.71, 129.66, 129.3, 129.0, 128.0: Ar CH; (79.87, 79.62) Boc tertiary; 68.22 (CH 2 OBn); 67.75 (CH 2 OBn); (61.67, 61.55) (CH); 59.39 (56.51, 55.82, 55.61) 53.11 (OCH 3 (45.56, 45.16, 44.73, 44.61, 44.43, 44.24, 43.42, 43.04) (2xCH 2 (40.77, 40.15, 40.03, 39.42, 39.27) (2xCH 2 (39.66, 32.60, 32.45, 31.44) (CH 2 29.04 (CH 3 Boc); 29.93 23.88 (CH 2 22.36 (CH 2 Mass Spectrum (ISMS) m/z 704.4 calculated for C 40

H

53

N

3 0 8 703.

Preparation of 74 and Bn NHBoc CO 2

CH

3 NHBoc CO2CH 3 74 major product 75 minor product The mixture of epimeric amines 73 (260 mg, 0.4 mmol) was dissolved in methanol (20 mL) and 10% palladium on carbon added (100 WO 99/48913 PCT/AU99/00207 41 mg). The solution was hydrogenated (40 psi H 2 at room temperature for 3 h to give the deprotected amino acid (MH'=480Da). After filtration, the solvent was removed and the residue (170 mg) was dissolved in DMF mL) and diluted with CH 2

CI

2 (50 mL). To this solution was added HBTU (180 mg, 0.48 mmol) and DIEA (150 mg, 1.2 mmol). After stirring for min at room temperature the solution was diluted with aq.NaHCO 3 the aqueous layer separated, and the organic layer washed with water (x3) then brine and then dried over MgSO 4 Evaporation of the solvent left an oil which was purified by flash chromatography eluting with 20-40% EtOAc in light petroleum ether. The product diastereomers were just separable under these conditions, with the minor diastereomer 75 eluting first to give a yield of 18% (30 mg) followed by the major diastereomer 74 in 50% (85 mg) yield. TLC EA:LP 1:1 Rf=0.43, 0.29. 1 H NMR (300 MHz,

CD

3 CN): 00 Isomer 75: 7.29, 4H, m, ArH; 7.22, 1H, m, ArH; 5.17, 1H, dd, J=6.5, 8.4 Hz; 5.08, 1H, m; 3.65, 3H, s, OCH 3 3.61, 1H, dd, J=11.4, 15.6 Hz; 3.27, 1H, ddd, J=1.5, 5.7, 15.9 Hz; 3.12, 1H, dd, J=4.5. 14.3 Hz; 2.98, 1H, bm; 2.72, 1H, m; 2.64, 1H, dd, J=9.9, 14.3 Hz; 2.57, 1H, bm; (2.17, H 2 1.68, 3H, m; 1.60, 1H, m, LeuO; 1.36, 9H, s, Boc; 1.16, 1H, m; 0.95, 3H, d, J=6.4 Hz, LeuO; 0.93, 3H, d, J=6.6 Hz. Isomer 74: 7.29, 4H, m, ArH; 7.22, 1H, m, ArH; 5.11, 1H, dd, J=5.6, 9.4 Hz; 4.29, 1H, br, NHBoc; 3.81, 1H, dd, J=4.6, 9.8 Hz; 3.65, 3H, s, OCH 3 3.59, 1H, dd, J=10.8, 15.2 Hz; 3.19, 1H, dd, J=5.5, 15.2 Hz; 3.13, 1H, dd, J=4.5, 13.8 Hz; 2.94, 2H, m's; 2.71, 1H, m; 2.64, 1H, dd, J=10.3, 13.3 Hz; (2.17, H 2 1.76, 1H, m; 1.69, 2H, m; 1.57, 2H, m; 1.36, 9H, s, Boc; 0.93, 6H, d, J=6.5 Hz. 13 C NMR (75 MHz, CDCI 3 0 Isomer 175.2; 172.5; 155.9; 138.9; 129.3; 128.5; 126.4; 79.2; 60.91; 60.62; 55.65; 52.19; 45.70; 43.98; 38.12; 37.99; 33.46; 28.30, Boc; 25.01; 23.10; 21.93. Isomer 74 175.1; 172.5; 155.7; 139.3; 129.3; 128.7; 126.8; 78.9; 56.01; 55.80; 53.05; 52.14; 42.07; 40.70; 38.01; 37.98; 31.51; 28.26, Boc; 25.03; 23.11 21.74. Mass Spectrum (ISMS) m/z 462.3 calculated for C 32

H

45

N

3 0 5 461 fragments (OR 406.2 (-tBu).

WO 99/48913 PCT/AU99/00207 42 Example Selective synthesis of the 5(S) diastereomer by the short method The 3(S)5(S) diastereomer, the minor product formed as described above, can be selectively synthesised by the use of an intramolecular reductive amination-cyclisation as described below: Ph Ph NHBoc H CHMe 2 ZHN F ZHN h H 2 /Pd-C H J NHBoc CHMe 2 N 76 O MeOH 0,N 2 Me DIPEA, O 71 C2Me DIPEA, CH 2 2 77 COMe Preparation of acyl fluoride 76: Z-phenylalanine acid fluoride was prepared by general literature methods (Carpino et al., 1990; Wenschuh et al., 1994) as follows: 1.1 equivalents of diethylaminosulfurtrifluoride (DAST) were added to ZPheOH in dry dichloromethane solution under nitrogen at 0 0

C.

After stirring for 15 min the reaction was worked up by pouring onto iced water and separating the organic layer, washing once with cold water and then drying over MgSO 4 The product was purified by precipitation from ether/petroleum ether and dried in vacuo. 'H NMR (300 MHz, CDCI 3 0 7.36, 8H, m's; 7.28, 2H, m; 5.30, 1H, bd; J=7.5 Hz, NH; 5.13, 2H, s,

OCH

2 Ph; 4.85, 1H, m, oH; 3.20, 2H, m, OH 2 1 3 C NMR (75 MHz, CDCl3): 0 161.8, d, 1 JcF= 37 0Hz; 155.5; 135.7; 134.2; 129.1; 129.0; 128.5; 128.3; 128.1; 127.7; 67.36; 53.50, d, 2 JcF=59 Hz; 36.70.

Preparation of 77: To the amine 71 (2.7 g, 8.2 mmol) dissolved in CH 2

CI

2 mL) was added Z-phenylalanine acid fluoride 76 (prepared as described above) (3.0 g, 10 mmol) and DIEA (1.3 g, 10 mmol) and the solution stirred at room temperature under nitrogen for 30 h. The solvent was then evaporated in vacuo and the residue dissolved in ether and WO 99/48913 PCT/AU99/00207 43 extracted in turn with 1M HCI 10% aq. Na 2

CO

3 then brine and then dried over MgSO 4 The solution was filtered and the solvent removed in vacuo. The resulting oil was purified by flash chromatography eluting with 20-40% ethyl acetate in light petroleum ether for a yield of about 80% of the target 77 as a clear oil. TLC 40%EA:LP Rf=0.40. 1

H

NMR (300 MHz, CDCl3): E 7.41-7.13, 10H, Ar; 5.48, 1H, bd, J=9.2 Hz, NHCbz; 5.19, 1H, bm, NHBoc; 5.09, 2H, s, OCH 2 Ph; 4.76, 1H, dt, J=6.4, 8.9 Hz, PheO; 4.38, 1H, dd, J=5.2, 9.3 Hz, LeuO; 3.92, 2H, d, Hz, GlyO; 3.60, 3H, s, OCH 3 3.54, 1H, m; 3.38, 1H, m; 3.08, 1H, dd, J=8.4, 13.3 Hz; 2.93, 1H, dd, J=6.1, 13.1 Hz; 2.65, 2H, m; 2.80, 1H, m; 2.64, 1H, m; 1.46, 9H, s, Boc; -1.38, 1H, m; 0.90, 6H, 2xd, J=6.6, LeuD. 13 C NMR (75 MHz, CDCl3) amide rotamers only the major peak of rotamer peak pairs is reported: 0 204.1; 172.1; 171.4; 156.7; 155.6; 136.2; 135.8; 129.4-127.1: ArCH; 79.8; 66.82; 58.15; 52.25; 52.05; 50.28; 41.32; 39.58 (2 coincident signals as determined by relative intensity, shift and the presence of both minor rotamer peaks); 37.82; 28.23, Boc; 24.67; 23.08; 21.67. Mass Spectrum (ISMS) m/z 612.3 (M+H calculated for C 33

H

45

N

3 0 8 611; fragments: (OR 556.3 512.3 (-Boc).

Selective preparation of 75 from 77: The ketone 77 (1mmol) was dissolved in 0.1M methanolic HCI (30ml) and 10% palladium on activated carbon (200mg) was added.

The solution was hydrogenated at 30 psi H 2 (room temperature) for 8 h and then diluted with aq. NaHCO 3 and extracted with ethyl acetate. The organic layer was washed with water (x2) and then brine then dried over MgSO 4 Filtration and removal of solvent in vacuo left the crude product in good yield and purity. Analysis of the crude product by NMR and by TLC did not reveal any of diastereomer 74. The reaction was estimated to be >95% stereoselective.

Example Synthesis of a biologically active D-turn mimetic for the Arg-Gly-Asp sequence WO 99/48913 WO 9948913PCT/AU99/00207 NHBoc .HCI 07 N 0 NHBoc

P

0 Ph) THF, DIPEA N NHBoc

H

(BocAsp(OBn)) THF, DIPEA I.TFACH 2 C1 2 2. NaB H(OAC) 3,

DCE

C02Bn

HN'

N P h

NH

2 c0 2 N Ph BocArg(Tas)OH BOP, CH 2 C1 2 DMF, DIPEA r Bockrg(Tos)HN HjC 2 Bn N Ph 81 Na, NH 3, -330iC BocArgHN 1. CH 2 0I 2 ,CF 3 CO 2

H

2. CH 3 C0 2 C0CH 3 C0 2 Bn

HN-

rlN Ph AcArg(Tos)HN 83 Ca 2 Na, NH ~HN JPh -330C N AcArgHN 84 Preparation of 78: The 0,El -unsaturated ketone 70 (1.0 g, 5.4 mmol, prepared as previously described) was reacted with phenethylamine hydrochloride (1.07 g, 6.8 mmol) and DIEA in THE by the method previously described for the preparation of 71. The crude product 78 was used without further purification for the next reaction. Mass Spectrum (ISMS) mlz 307.2

(MH

4 calculated for C 17

H

26

N

2 0 3 306; fragments (OR 60): 250.9 (4t3u).

Preparation of 79: To a stirred solution of Boc-aspartic acid Ei-benzyl ester (3.23 g, 10 mmol) in CH 2

CI

2 (10 mL) was added dicyclohexylcarbodiimide mL of 0.5M solution in CH 2

CI

2 at room temperature. A copious precipitate of dicyclohexylurea soom formed; after 10 min the solution was filtered, and the solvent removed in vacua. The residual oil was WO 99/48913 PCT/AU99/00207 added to a solution of crude 78 (1.3 g) in THF, followed by DIEA (645 mg, mmol), and the solution stirred for 4 h. The reaction mixture was diluted with ether/ethyl acetate and washed with 1M HCI, aq. NaHCO 3 water, brine and dried over MgSO 4 The crude product was purified by flash chromatography eluting with 30-50% ethyl ether in petroleum ether to give a reasonable yield of 79 (estimated as 80% based on 78) as a clear oil. 1 H NMR (300 MHz, CDCl3, amide rotamers present): DO 7.38-7.16, m, Ar; 5.37, 1H, bd, J=9 Hz, AspNHBoc (minor rotamer 5.33, Hz); 5.25, m, 1H (Gly NH); 5.10, 2H, m, OCH 2 Ph; 4.89, 1H, m; 3.93, 2H, d, J=4.4 Hz, GlyO; 3.67-3.53, 3H, m's; 3.47, 1H, m; 2.95-2.52, 6H, m's (including 2.88, 2H, m; 2.63, 2H, ABX, J=15.8, 7.3, 5.8 Hz, OH 2 Asp); 1.44, 18H, multiple singlets, 2xBoc. 13 C NMR (75 MHz, CDCI 3 7 (major rotamer only) 204.7; 171.0; 170.3; 155.6; 154.8; 137.7; 135.5; 128.9, 128.6, 128.5, 128.2, 126.6: ArCH; 80.06; 79.73 (2x tBoc); 66.57; 50.55; 50.33; 46.99; 42.24; 37.69 (2 signals); 35.50; 28.22 (2x Boc). Mass Spectrum (ISMS) m/z 612.3 calculated for C 3 3

H

4 5

N

3 0 8 611 fragments (OR 60): 556.1 512.1 (-Boc).

Preparation of The ketone 79 (390 mg, 0.64 mmol) in CH 2

CI

2 (2 mL) was treated with trifluoroacetic acid (2 mL) and the solution stirred for 30 min at room temperature. The volatiles were then removed in vacuo and

CH

2

CI

2 (3 mL) added and removed in vacuo The residual oil was dissolved in 1,2-dichloroethane (5 mL) and NaBH(OAc) 3 (270 mg, 1.3 mmol) added. The mixture was stirred for 20 min then the solvent removed and the residue dissolved in ethyl acetate and washed with aq.

Na 2

CO

3 and then brine and then dried over MgSO 4 The crude product (after solvent removal 210 mg, 84%) was of good purity by MS and NMR, with only one diastereomer observed diastereoselectivity).

1 H NMR (300 MHz, CDCl3): C 7.39-7.10, 10H, m, Ar; {5.20, 5.16, 5.14, 5.10}, 2H, ABq, J=12.5 Hz) OCH 2 Ph; 3.86, 1H, t, J=6.3; 3.76-3.43, 3H, m's; 3.14, 1H, bdd, J=15, 5 Hz; 2.98-2.76, 5H, e; 2.70, 1H, dd, J=7.4, 16 Hz; 2.46, 1H, m; 1.64, 1H, bm; 1.06, 1H bm. 13C NMR (75 MHz, WO 99/48913 PCT/AU99/00207 46 CDC13): 173.9; 172.0; 138.9; 135.9; 128.7, 128.4, 128.0, 126.3: Ar; 66.16; 60.49; 56.55; 51.24; 48.39; 45.14; 38.05; 34.15; 33.01. Mass Spectrum (ISMS) m/z 396.2 calculated for C 23

H

2 9

N

3 0 3 395.

Preparation of 81: The crude amine product 80 (140 mg, -0.35 mmol) was coupled with BocArg(Tos)OH (182 mg, 1.2 eq) using the BOP reagent (188 mg) and DIEA (55 mg) in DMF/CH 2

CI

2 (5ml). The CH 2

CI

2 was evaporated in vacuo and the residue partitioned between diethyl ether/ethyl acetate and aq. NaHCO 3 The aqueous layer was separated and the organic layer washed in turn with 1M HCI water aq.

NaHCO 3 brine, and then dried over MgSO 4 Filtration and removal of the solvent in vacuo left the crude product amide 81 which was purified by flash chromatography eluting with 5-10% ethanol in ethyl acetate (yield 260 mg, TLC 10% EtOH in EtOAc Rf=0.38. 1 H NMR (300 MHz,

CD

3 OD): D 7.74, 2H, d, J=7 Hz; 7.4-7.15, 12H, m's; 5.15, 2H abq, J=11 Hz, OBn; 4.26, 1H, m; 4.03, 1H, m; 3.73, 2H, m; 3.48-3.07, 7H, e; 3.07, 1H, m; 2.92-2.73, 3H, m's; 1.92, 1H, m; 1.73, 1H, m; 1.66-1.45, 4H, e; 1.42, 9H, s, Boc. 13 C NMR (75 MHz, CD30D): 0 176.1; 172.5; 172.0 158.8; 158.1; 143.7; 142.2; 140.3; 137.5; 130.4; 130.1; 129.72; 129.68; 129.4; 128.4; 127.6; 127.3; 127.2; 80.92 67.75 (CH 2 62.55 57.27 56.00 52.55 (CH 2 48.74 (CH 2 44.42

(CH

2 41.22 (br, CH 2 37.00 (CH 2 35.10 (CH 2 32.41 (CH 2 30.15

(CH

2 28.87 (Boc CH 3 27.24 (br, CH 2 21.57 (CH 3 Mass Spectrum (ISMS) m/z 806.4 calculated for C41H 55

N

7 0 8 S: 805.

Preparation of 82: The amine 81 (50 mg, 0.06 mmol) in THF (0.6 mL) was cooled in a dry ice acetone bath and ammonia gas added until -30 mL of ammonia had condensed. Small pieces of sodium metal (3-6 mg) were added until the blue colour persisted. The reaction was quenched by the addition of ammonium carbonate (25 mg), the dry ice bath removed and the solvent allowed to evaporate at room temperature. The residue (which gave a crude mass spectrum with the product mass as the only WO 99/48913 PCT/AU99/00207 47 significant peak) was purified by reversed phase HPLC (Vydac C18) eluting with 85% solvent A CF 3 COOH in H 2 0):15% solvent B

CF

3 COOH and -10% H 2 0 in CH 3 CN) for 2 minutes followed by a 2%/min gradient. Only one product diastereomer was observed in the HPLC traces. Mass Spectrum (ISMS) m/z 562.3 calculated for

C

27

H

43

N

7 0 6 Preparation of 83: The amine 81 was dissolved in CH 2

CI

2

/CF

3

CO

2 H (2ml, 1:1) and stirred at room temperature for 30 minutes after which the Boc group had been removed. 10ml of CH 2

CI

2 was then added and the volatiles removed in vacuo (repeat once). The residue was again dissolved in

CH

2

CI

2 and acetic anhydride (2 eq.) added along with diisopropylethylamine (DIEA, 5 and the reaction stirred at room temperature for 2 h. The volatiles were removed in vacuo and the residue dissolved in ethyl acetate and washed with aq. NaHCO 3 then brine and then dried over MgSO 4 Filtration and removal of the solvent in vacuo left the crude product 83 as an oil in reasonable purity. The 1 H NMR was badly broadened in common solvents at room temperature. 13C NMR

CDCI

3 0 173.7; 172.4; 171.9; 171.0; 157.0; 142.1; 140.4; 138.8; 135.8; 129.2, 128.7, 128.4, 128.1, 128.0, 126.3, 125.8: ArCH; 66.22, OCH 2 Ph; 60.08, CH; 56.09, CH; 52.94, br, CH; 51.06, CH 2 48.21, CH 2 44.31, CH 2 40.13, br, CH 2 37.79, CH 2 34.16, CH 2 32.97, CH 2 (29.59, 29.50) 1C, br, CH 2 25.64, br, CH 2 22.91, CH 3 21.32, CH 3 Mass Spectrum (ISMS) m/z 748.2 calculated for

C

37

H

4 9

N

7 0 7 S: 747.

Preparation of 84: Compound 84 was prepared from 83 by dissolving metal reduction as described for the preparation of 82 above. Purification was carried out by HPLC under the same conditions as for 82.

Testing of Arg-Glv-Asp mimetics 82 and 84 for inhibition of platelet aggregation in human platelet rich plasma (PRP) WO 99/48913 PCT/AU99/00207 48 The peptide sequence arginine-glycine-aspartic acid (RGD) is important to the binding of proteins to certain integrin receptors, such as the GPIIb-Illa receptor found on the surface of platelets. Several cyclic peptides having the RGD sequence have been found to antagonise the binding of plasma proteins to the GPIIb-IIIa receptor, thereby inhibiting blood clotting. GPIIb-IIIa antagonists have therapeutic potential as antithrombotics, there are several in early clinical trials(Humphries, Doyle et al., 1994). Mimetics based on O-turn structures centred on the Asp residue have been successful, this structure was chosen to test the compounds of the invention.

Solutions of the compounds to be tested were made up in water. Platelet aggregation induced by adenosinediphosphate (ADP, in human PRP was measured by the decrease in light scattering on aggregation, measured with a platelet aggregometer. The tetrapeptide Ac-Arg-Gly-Asp-Ser-NH 2 was used as a positive control.(Callahan et al., 1992) Compounds 82 and 84 were both found to inhibit platelet aggregation in a dose dependent manner, and both exhibited stronger inhibition than the control peptide. Compound 84 was the strongest, having inhibitory activity approximately five times more potent than Ac-Arg-Gly-Asp-Ser-NH2 under the conditions of the test.

Example Synthesis of fully substituted O-turn mimetics for the Phe-Leu-Ala sequence in both the 4(R) and 4(S) configurations The synthesis up to the final common intermediate for the 4(R) and 4(S) diastereomers, the aldehyde 93, is summarised below:- WO 99/48913 WO 9948913PCT/AU99/00207 LiAN 4, Et 2 0 -t CH 3

OCH

3 Ph N

H

Boc 0 86 Boc 1 0 H 2 Wfc C 2 H 3 CH 2 CI 2 MgSO 4 L iOH, H 2 0, MeD H (recrystallise to required purity)

CH

2 O, EtOAc

-H

2 0 N

H

Boc 87 C0 2 Me B-aIlyl-9BBN (Rgla) CH 2 CI~ 2 lether, -780OC

CS

2

CO

3 DM F, BnBr N r 88 C0 2 Me diastereomers 80:20 R:S P h

N

Boc

+H

s0 C0 2 OsO 4

/NMO

H 5 10 6 TH F .Ph Bocphenylalanine N,O-dimethylhydroxylamide 85 was synthesised by the general solution phase coupling procedure as previously described from Boc-phenylalanine and N,O-dimethyl hydroxylamine hydrochloride. Yield: -quantitative. Purification: on a short silica column eluting with ether. IH NMVR (300 MHz, CDCI 3 El 7.33-7.12, 5H, m, Ar; 5.20, 1H, bd, J-7 Hz, NH; 4.95, 1H, bin, Pheol; 3.66, 3H, s, OCH 3 3.17, 3H, s, NCH 3 3.06, 1 H, dd, J=6, 13.5 Hz, PheE]; 2.88, 1IH, dd, J=7.5, 13.5 Hz; 1.40, 9H, s, Boc. 13 C NMVR (75 MHz, CDC1 3 El 172.2; 155.1; 136.5; 129.4; 128.2; 126.7; 79.5; 61.4; 51.4; 38.8, Pheol; 32.0; 28.2, Boc.

The amide 85 was reduced to Bocphenylalanine aldehyde 86 by the method of Fehrentz and Castro (Fehrentz and Castro, 1983) Briefly: amide (2 minol) dissolved in dry ether (20 mL) and cooled and in WO 99/48913 PCT/AU99/00207 an ice bath under nitrogen, then LiAIH 4 (95 mg, 2.5 mmol) added and stirring continued 15 min. Then KHSO 4 (477 mg, 3.5 mmol) in 10 mL water added and then 150 mL ether and wash with 1M HCI (cold) aq.

NaHCO 3 brine, and dried over MgSO 4 Removal of the solvent left the solid aldehyde in -90% crude yield containing some of the overreduced alcohol as the only significant impurity. TLC EtOAc:light pet. Rf=0.5. 1

H

NMR (300 MHz, CDCl3): 0 9.63, 1H, s, aldehyde; 7.37-7.13, 5H, m, Ar; 5.07, 1H, bs, NH; 4.43, 1H, m, PheO; 3.11, 2H, d(AB) PheO; 1.43, 9H, s, Boc. 13 C NMR (75 MHz, CDCI 3 0 199.4, aldehyde; 155.3, carbamate; 135.7, ipso; 129.3, 128.7, 127.1: ArCH; 80.2, tBoc; 60.8, PheO; 35.5, PheO; 28.2, Boc Methyl leucinate hydrochloride (0.80 g, 4.4 mmol) was neutralised with 10% aq. Na 2

CO

3 solution (25 mL), and the solution was mixed with brine (25 mL) and extracted with CH 2

CI

2 (3x20 mL). The organic extracts were dried over MgSO 4 and most of the solvent removed under vacuum mL residue). This solution of methyl leucinate was added to Boc phenylalanine aldehyde 86 (1.1 g, 4.4 mmol) in CH 2

CI

2 mL), the stirred solution soon became turbid due to the separation of water, dried MgSO 4 (500 mg) was added and the solution cleared. After 30 min the solution was filtered into a dried flask under nitrogen. NMR analysis showed that all the aldehyde had been converted to the imine 87 and that significant racemisation had not taken place. The imine was used without further purification for the allylation reaction. 1 H NMR (300 MHz, CDCI 3 0 7.61, 1H, d, J=1.3 Hz, imine; 7.32-7.14, 5H, m, Ar; 5.69, 1H, bd, J=4.5 Hz, NH; 4.49, 1H, m, Phel; 3.85, 1H, dd, J=5.5, Hz, LeuO; 3.69, 3H, s, OCH 3 3.20, 1H, dd, J=5.0, 14.5 Hz, PheO; 2.96, 1H, dd, J=8.0, 13.5 Hz; PheO; 1.63, 1H, m; 1.46, 9H, s, Boc; 1.42, 1H, m; 1.30, 1H, m; 0.88, 3H, d, J=6.5 Hz, LeuD; 0.80, 3H, d, J=6.5 Hz, LeuD. 13 C NMR (75 MHz, CDCl 3 O 171.7, ester; 164.3, CH, imine; 154.6, carbamate; 136.1, ipso; 128.9, 127.7, 126.0: ArCH; 78.56, tBoc; 69.51; 54.08; 51.32; 41.02, CH 2 38.04, CH 2 27.73, Boc; 22.35; 22.48; 20.63.

WO 99/48913 PCT/AU99/00207 51 B-allyl-9-borabicyclononane Rgla can be synthesised from B-methoxy-9-borabicyclononane (synthesised in turn from the methanolysis of 9-BBN (Kramer and Brown, 1974)) by the method of Kramer (Kramer and Brown, 1977). Alternatively the following one-pot synthesis from 9-BBN was used: a suspension of 9-BBN (crystalline dimer, 8.97 g, 73.5 mmol) in anhydrous ether (75 mL) was stirred under nitrogen and cooled to 0°C. Methanol (3.3 mL, 81 mmol) was slowly added by syringe (gas evolved), and vigorous stirring continued for ~3 h (9-BBN gradually dissolves, gas evolution ceases). Allylmagnesium bromide in ether (81 mL of a 1.0M solution) was slowly added to the solution (still cooled to (a thick grey ppt. forms, stirring may be difficult). Stirring was continued for 1 h then the solution was allowed to warm to room temperature and the ether was pumped off under moderate vacuum (-300->20mbar). The residue was re-suspended in anhydrous hexane (100 mL) and then stirring stopped to allow the magnesium salts to settle out. The solution was estimated by reaction with a known amount of methylphenylketone in ether (found to be -0.57M, equal to 78% yield). The clear solution of B-allyl-9-BBN was used directly for allylation of the imines. (This procedure was adapted from one described by Rachlera and Brown (Racherla et al., 1992)) The imine 87 (-23 mmol) was dissolved in dry diethylether (100 mL) under nitrogen and the stirred solution cooled to -78 0 C. B-allyl-9-BBN (47.5 mL of -0.57M solution in hexane, -27 mmol) was added and the solution stirred for 1 h and then allowed to warm to room temperature with stirring for an additional 1 h.

Glacial acetic acid (1.5 mL) was added and the ether was removed in vacuo. The residue was dissolved in acetonitrile (100 mL) and more glacial acetic acid (5 mL) added. The solution was then refluxed until all of the borane adduct had been converted to the amine (-24 h, monitored by TLC: Rf adduct>Rf amine 0.32 in 1:5 EtOAc:light pet.). The acetonitrile was removed in vacuo and the residue partitioned between ether/light petroleum and 10% aq. Na 2

CO

3 The organic layer was washed again with 10% aq. Na 2

CO

3 and then extracted with a solution of WO 99/48913 PCT/AU99/00207 52 methanol in 0.5M HCI (three times), the organic layer containing the neutral reaction products g) was discarded. The aq. acid extracts were immediately neutralised with solid NaHCO 3 and then extracted with ether. The ether solution was washed with water then brine and then dried over MgSO4. Evaporation of the solvent left the amine products (5.9 g) which were further purified by flash chromatography eluting with 7.5-15% ethyl acetate in light petroleum for a yield of 50+% of the amines 88 based on the crude aldehyde 86 used in the imine formation. Some separation of the diastereomers was observed in the chromatography, but they were not well resolved. Alternatively the crude amines were hydrolysed to the amino acid as described below and purified by recrystallisation. 1H NMR (300 MHz, CDCI 3 major diastereomer: E 7.32-7.13, 5H, m, Ar; 5.84, 1H, m, vinylCH; 5.11, 2H, m, vinylCH 2 5.00, 1H, d, J=8 Hz, NHBoc; 3.88, 1H, m, Phen; 3.66, 3H, s, OCH 3 3.40, 1H, t, J=7 Hz, LeuO; 2.87, 1H, dd, J=5, 13 Hz, PheE; 2.69, 2H, m's: PheO+CH(homoallyl); 2.23, 2H, m, allyl; 1.7, 1H, b, NH(amine); (1.65, 1H, m; 1.47, 2H, m) LeuO+0; 1.33, 9H, s, Boc; 0.90, 6H, t(2 doublets) J=7, 7 Hz, LeuO. 13C NMR (75 MHz, CDCl3), major isomer: 0 176.1; 155.4; 138.6, ipso; 135.2, CH vinyl; 129.2, 128.2, 126.1: CHAr; 117.4,

CH

2 vinyl; 78.8, tBoc; 58.94; 58.56; 54.10; 51.71; 42.87; 36.52; 35.61; 28.24, Boc; 24.78; 22.68; 22.23. Mass Spectrum (ISMS) m/z 419.2 calculated for C 32

H

45

N

3 0 5 418 fragments (OR 65): 363.2, (-tBu).

The crude amine product 88 (1.7 g, -4 mmol) was dissolved in methanol/water and LiOH.H 2 0 (800 mg, 19 mmol) added. The solution was stirred at room temperature until the hydrolysis was complete (12 h) and then neutralised with 1M HCI (19 mL). On standing a copious white precipitate formed which was filtered off and washed with water. The solid was recrystallised from ethanol-water to give fine needles of (mainly) the major diastereomer 89 (first crop 1 m.p.:175-177 0 C. The product was further recrystallised as required. 1 H NMR (300 MHz,

CD

3 OD): (ref. 3.31 ppm) 7.33-7.18, 5H, m; 5.90, 1H, m; 5.35. 1H, d, WO 99/48913 PCT/AU99/00207 53 J=17.1 Hz; 5.26, 1H, d, J=10.2 Hz; 4.31, 1H, m; 3.65, 1H, dd, J=5.7, 7.9 Hz; 3.27, 1H, m; 2.92, 1H, dd, J=5.2, 14.0 Hz; 2.76, 1H, dd, J=10.1, 14.0 Hz; 2.59, 1H, m; 1.82, 1H, m; 1.37, 9H, s, (Boc); 0.97, 3H, d, J=7 Hz; 0.94, 3H, d, J=7 Hz. 13C NMR (75 MHz, CD30D): 0 (ref. 49.15 ppm) 173.7; 159.4; 138.8; 134.5; 130.33; 129.8; 128.0; 120.5; 81.34; 63.65; 55.84; 41.19; 37.90; 32.70; 28.78; 26.11; 23.56. Mass Spectrum (ISMS) m/z 405 (MH calculated for C 23

H

36

N

2 0 4 404.

The amino acid 89 was esterified to 90 by the method of Bodansky and Bodansky (Bodansky and Bodansky, 1984) as follows: the amino acid 89 (400 mg, 1 mmol) was dissolved in methanol/water and neutralised with Cs 2

CO

3 (300 mg), then the solvents were removed in vacuo, then DMF added and removed in vacuo. The residue was dissolved in DMF (10 mL) and benzyl bromide (190 mg, 1.1 mmol, purified by passage through a short column of basic alumina) added to the stirred solution. After 2 h the reaction was diluted with aq. NaHCO 3 and extracted with 1:1 EtOAc:light pet. The organic layer was washed in turn with aq.NaHCO 3 water brine and then dried over MgSO 4 Evaporation of the solvent left the product 90 as a clear oil which solidified to a low melting solid ~55 0 C) on standing (500 mg, TLC 25%EtOAc in light pet. Rf=0.57. 1 H NMR (300 MHz,

CDCI

3 0 7.38-7.32, 4H, m; 7.28-7.14, 6H, m; 5.82, 1H, m; 5.19-5.05, 4H, m's, (OBn ABq, J=12.5 Hz, Oa=5.1 6 Ob=5.

12 ppm); 4.9, 1H, br; 3.88, 1H, br; 3.44, 1H, bt, J=7 Hz; 2.88, 1H, dd, J=5, 14 Hz; 2.77-2.60, 2H, bm; 1.63, 1H, m; 1.56-1.35, m, 2H; 1.33, 9H, bs (Boc); 0.88, 3H, d, J=6.5 Hz; 0.85, 3H, d, J=6.5 Hz. 13C NMR (75 MHz, CDCI 3 0 175.5; 155.5; 138.6; 135.8; 135.2; 129.2; 128.5; 128.2; 126.1; 117.4; 78.90; 66.40; 58.96; 58.49; 54.25; 42.83; 36.33; 35.71; 28.27 (Boc); 24.77; 22.63; 22.32. Mass Spectrum (ISMS) m/z 495 calculated for C 30

H

42

N

2 0 4 494.

The amine 90 (500 mg, 1 mmol) was dissolved in ethyl acetate (20 mL) and 37% aqueous formaldehyde solution (0.5 mL) was added. The solution was stirred for 12 h and then diluted with light WO 99/48913 PCT/AU99/00207 54 petroleum (40 mL) and washed in turn with aq. NaHCO 3 water (x2) and brine and then dried (MgSO 4 Removal of the solvent in vacuo gave the product 91 as a clear oil in approximately quantitative yield. Further purification was carried out by flash chromatography eluting with ethyl acetate in light pet. 1 H NMR (500 MHz, CD 3 CN): (rotamers were present in a ratio of 7:3) 7.36, 4H,m, Ar; 7.27-7.11, 6H, Ar; 5.70, 1H, m, vinyl CH; 5.17-4.97, 4H, m's, vinyl CH 2 and OCH 2 Ph; 4.44, 0.7H, d, J=5.0 Hz, ring CH 2 major rotamer; 4.33, 0.3H, d, J=4.4 Hz, ring

CH

2 minor rotamer; 4.19, 0.7H, d, J=5.0 Hz, ring CH 2 major rotamer; 4.09, 0.3H, d, J=4.6 Hz, ring CH 2 minor rotamer; 4.06. 0.3H, m, PheO, minor; 4.02, 0.7H, m, PheO, major; 3.74, 0.7H, dd, J=9.8, Hz, and 3.69, 0.3H, m, LeuD; 3.10, 1H, m, ring methine (homoallyl); 2.88, 0.3H, m, PheO(a); 2.84, 0.7H, dd, J=4.1, 13.4, PheO(a); 2.72.

0.3H, dd, J=6.5, 13.5, PheO(b); 2.65, 0.7H, dd, J=9.5, 13.2, PheO(b); 2.49, 1H, m, allyl(a); 2.15, 1H, m, allyl(b); 1.76-1.42, 3H, m's, LeuOD+D 1.33, 2.5H, s, Boc, minor rotamer; 1.09, 6.5H, s, Boc, major rotamer; 0.97-0.84, 6H, d's, LeuD (major rotamer: 0.94, J=6.3 Hz; 0.90, J=6.2 Hz).

13 C NMR (75 MHz, CD 3 CN), only major rotamer reported except where indicated: 0 (ref. 118.69 ppm) 173.3; 154.2; 140.9; 137.8; 136.3 (CH); 131.3; 129.9; 129.7; 129.6; 129.5; 127.2; 118.2 (CH 2 79.98 (Boc tertiary); 67.17 (CH 2 63.49 62.47 (CH 2 60.91 57.68 40.34 (CH 2 36.04 (CH 2 33.18 (CH 2 (29.08 Boc minor rotamer); 28.61 (Boc major rotamer); 25.98 23.79 (CH 3 22.36

(CH

3 Mass Spectrum (ISMS) m/z 507 calculated for C31H 4 2

N

2 0 4 506.

The alkene 91 was dihydroxylated with OsO4/Nmethylmorpholine-N-oxide in tBuOH/water as previously described for the dihydroxylation of 60. The crude product 92 was used directly in the next reaction. TLC 1:1 EtOAc:light pet. Rf=0.36. Mass Spectrum (ISMS) m/z 541 (M+H calculated for C31H 44

N

2 0 6 540.

The glycol 92 (87 mg, 0.16 mmol) was dissolved in THF (4 mL) and H 5 10 6 (37 mg, 0.16 mmol) dissolved in THF (3 mL) was added WO 99/48913 PCT/AU99/00207 and the reaction stirred at room temperature. A precipitate of iodic acid rapidly formed and the reaction was complete in <5 min. The THF solution was diluted with ether and washed in turn with 10% aq.Na 2

CO

3 water, brine and then dried (MgSO 4 The product aldehyde 93 was of good purity but was not particularly stable to storage. Any traces of acid must be rigorously excluded to prevent isomerisation to the trans isomer.

A portion was purified by flash chromatography, eluting with 15%EtOAc in light petroleum. TLC 15%EtOAc in light pet. Rf=0.27. The yield was good Amide rotamers were evident in the NMR spectra, ratio only the peak due to the main rotamer is reported unless otherwise noted. 1 H NMR (300 MHz, CD 3 CN, ref 1.94 ppm): D 9.53, 1H, s; 7.42- 7.10, 10H, m's; 5.11, 2H, s, (OCH 2 Ph); 4.41, 1H, br; 4.25, 1H, q, J=6.3 Hz; 4.15, 1H, br; 3.56, 1H, dt, J=8.5, 5.7 Hz; 3.54, 1H, bm; 2.90-2.58, 4H, m; 1.75-1.45, 3H, bm; 1.37, bs, Boc minor rotamer; 1.20, bs, Boc major rotamer; 0.92, 3H, d, J=6 Hz; 0.88, 3H, d, J=5.7 Hz. 13 C NMR MHz, CD 3 CN, ref 118.69 ppm): E 202.0; 173.1; 154.2; 140.4; 137.6; 131.1; 129.9; 129.62; 129.55; 127.26; 80.28 (Boc tertiary); 67.31

(CH

2 61.90 (CH 2 60.43 58.56 57.95 43.75 (CH 2 40.36 (CH 2 36.48 (CH 2 28.66 (Boc); 25.83 23.67 (CH 3 22.25

(CH

3 Mass Spectrum (ISMS) m/z 509 calculated for C3 0

H

40

N

2 0 5 508.

Conversion of 4,5-cis aldehyde 93 to the 4,5-cis 4(S) amine product was completed by a two step reductive amination procedure as illustrated below:

CH(CH

3 2

CH(CH

3 2

CH(CH

3 2 L CO 2 Bn K CO 2 Bn L C 2 BnH N .0 N. N N NI HAlaOCH 3 CO 2

CH

3 NaBH4 COCH Bn N Bn NeH '"Bn Boc MeOH Boc" MeOH Boc 93 imine not isolated 94 WO 99/48913 PCT/AU99/00207 56 Alanine methyl ester hydrochloride (120 mg, 0.86 mmol) was dissolved in 1:1 brine:10%aq.Na 2

CO

3 and extraction into CH 2

CI

2 (x2).

The organic extracts were dried (MgSO4), filtered and the majority of the solvent removed in vacuo to leave the volatile amine which was added to a solution of the freshly prepared aldehyde 93 (100 mg, 0.2 mmol) dissolved in methanol mL, strictly acid free). The solution was stirred at room temperature for 2 h whereupon analysis of a test portion reduced with NaBH 4 showed imine formation to be complete (none of the alcohol formed on reduction of aldehyde was detected). Solid NaBH 4 (50 mg, 1.3 mmol) was added to the solution and stirring continued for 10 min and then the reaction partitioned between ethyl acetate and a 2

CO

3 mixture. The aqueous phase was separated and the organic layer washed with water (x2) then brine and then dried (MgSO 4 NMR analysis of the crude product failed to detect the corresponding trans diastereomer Evaporation of the solvent left an oil which was purified by flash chromatography eluting with 20-40% EtOAc in light petroleum for a 60-70% yield of 94. TLC pet. Rf=0.43. Rotamers observed in the NMR spectra, ratio -3:1, separate signals due to the minor rotamer recorded only where indicated.

1 H NMR (300 MHz, CD 3 CN, ref. 1.94 ppm): 0 7.37, 4H, 7.3-7.1, 6H, m; 5.12, 5.09: 2H, ABq, J=12 Hz; 4.39 (major rotamer), 4.29 (minor): 1H, d, J=5 Hz; 4.15, 1H, J=5 Hz; 4.06, 1H, m, PheHO; 3.75-3.57, 4H, m, LeuHO+OCH 3 3.25-3.10, 1H, m; 3.03, 1H, m; 2.87-2.60, 2H, m, Phen; 2.52-2.25, 2H, m; 1.81, 1H, m; 1.67, 1H, m; 1.6-1.38, 2H, m; 1.34, bs, Boc minor rotamer; 1.19, m, AlaO; 1.15, bs, Boc major rotamer; 0.93, 3H, d, J=6.6 Hz; 0.89, 3H, d, J=6.3 Hz. 13C NMR (75 MHz, CD 3 CN, ref.

118.69 ppm): 0 177.3; 173.4; 154.2; 141.0; 137.7; 131.2; 130.9; 129.9; 129.7; 129.6; 129.5; 127.1; 80.02 (Boc tertiary); 67.18 (CH 2 62.55 62.25 (CH 2 60.75 57.67 (2xCH, coincident signals); 52.55 (OCH 3 45.96 (CH 2 40.96 (CH 2 36.15 (CH 2 29.00 (Boc, minor rotamer); 28.73 (CH 2 28.62 (Boc, major rotamer); 25.96 (CH); WO 99/48913 PCT/AU99/00207 57 23.66 (CH 3 22.35 (CH 3 19.7 (CH 3 Mass Spectrum (ISMS) m/z 596 calculated for C 34

H

50

N

3 0 6 595.

Reductive amination of aldehyde 93 (or the isomer) with NaBH(OAc) 3 in dichloroethane gave rise to a mixture of products 94 and 95 in the ratio 1:9.

CO

2 Bn RCO 2 Bn H RCOBn {o c k HAlaOCH 3, N N ,B N' O NaBH (OAc) 3 C02CH 3 2CH3

S

B

DCE,AcOH N Bn Bn Boc Boc Bn 93 94 1:9 R=CH 2 CH(C H3).

The aldehyde 93 (50 mg, 0.1 mmol) was dissolved in 1,2dichloroethane (5 mL) and alanine methyl ester equivalents) and acetic acid (1drop, ~14 mg) were added. The mixture was stirred at room temperature for 5 min and then NaBH(OAc) 3 (40 mg, 2 eq.) was added and stirring continued for 30 min. The solvent was then removed in vacuo and the residue partitioned between EtOAc and 10% aq. Na 2

CO

3 the organic layer was washed with water and brine and then dried (MgSO 4 The product contained both diastereomers in the ratio trans:cis.

The products were purified by flash chromatography eluting with 20-45% EtOAc in light petroleum. TLC 40% EtOAc:light pet. Rf=0.43 (minor diastereomer, 94, cis), 0.23 (major diastereomer, 95, trans). Combined yield Rotamers were not observed although significant peak broadening was present, as observed for the corresponding trans aldehyde. The configuration of the major product was determined by NMR (NOESY experiment). 1 H NMR (300 MHz, CD 3 CN, ref 1.94 ppm): G 7.24-7.14, 10H, m's; 5.13, 2H, s, OCH 2 Ph; 4.38, 1H, br, ring methylene(i); 3.97, 1H, bd, ring methylene(ii); 3.61, 3H, s, OCH 3 3.75, 1H, ddd, J=2.7, 4.3, 8.7 Hz, PheHE; 3.50, 1H, m, LeuHO; 3.13, 1H, m, PheC'H(ring); 2.97-2.88, 2H, m, AlaHO+PheHO(i); 2.72, 1H, dd, J=2.9, 8.7 Hz, PheHO(ii); 2.33, 1H, ddd, J=11.5, 7.3, 5.5 Hz, CH 2 NH(bridge)(i); WO 99/48913 PCT/AU99/00207 58 1.98, 1H, m (dt, overlaps with solvent peak), CH 2 NH(bridge)(ii); 1.53, 2H, m, Leun+D; 1.43, 9H(s)+1H(m), Boc+LeuD; 1.35, 1H, m, bridge CH 2 1.29, 1H, m, bridge CH 2 1.06, 3H, d, J=7.0 Hz, AlaE]; 0.88, 6H, m, LeuO. 13 C NMR (75 MHz, CD 3 CN, ref 118.69 ppm): 0 177.2; 174.6; 154.5; 140.1; 137.6; 131.0; 129.9; 129.7; 129.6; 127.6; 80.61 (Boc tertiary); 67.50 (CH 2 63.62 (CH 2 63.5 (CH, br); 62.4 (CH, v.br); 60.67 57.70 52.47 (CH 2 45.15 (CH 2 40.65 (CH 2 v.br); 39.76 (CH 2 32.81 (CH 2 29.00 (CH 3 Boc); 26.21 23.47 (CH 3 22.88 (CH 3 19.62 (CH 3 Mass Spectrum (ISMS) m/z 596 calculated for C 34

H

49

N

3 0 6 595.

The diastereomeric amines were converted to the protected O-turn mimetic compounds 96 and 97 as described below:

CH(CH

3 2

CH(CH

3 2

H

2 /Pd-C N N 2 BOP, DIPEA BocN 94 EtOAc, EtOH N C02 3 DCM/DMF Bn' Boc Bn 96 CO 2

CH

3

CH(CH

3 2 C H(CH C H(CH 3)2 C0 2 H 2+ 0

H

2 /Pd-C CO1N BOP, DIPEA BocN O1_.

0CH3 N- EtOAc, EtOH "'DCM/DMF Boc 97 O 2

CH

3 The 4,5-cis amine 94 (42 mg, 0.07 mmol) was dissolved in ethyl acetate:ethanol 10:3 (13 mL) and 35 mg of 10% palladium on activated carbon was added and the mixture hydrogenated at 32 psi H 2 for 3 h to deprotect the benzyl ester to the amino acid (MH' 506 Da).

The solution was filtered and the solvent removed in vacuo, then the residue was dissolved in DMF (2 mL) and diluted with CH 2

CI

2 (15 mL) and DIEA (50 mg, -0.4 mmol) and BOP reagent (50 mg, 0.11 mmol) were added to the stirred solution at room temperature. The cyclisation was complete within a few minutes; the CH 2

CI

2 was then removed in vacuo WO 99/48913 PCT/AU99/00207 59 and the residue diluted with ethyl acetate and washed in turn with aq.Na 2

CO

3 /brine, water brine and then dried (MgSO 4 and the solvent removed in vacuo to leave a clear oil which was purified by flash chromatography eluting with 20% EtOAc in light petroleum for a yield of 25 mg of 96. TLC 1:1 EtOAc:light pet -0.45 The NMR spectra in

CD

3 CN at room temperature were significantly broadened indicating a degree of conformational interconversion slow on the NMR timescale. 1

H

NMR (300 MHz, CD 3 CN): 0 7.32-7.15, 5H, m, Ar; 4.88, 1H, q, J=7.1 Hz, AlaO; 4.20, 1H, bd, J=4.8 Hz, NCH 2 4.13, 1H, m, PheO; 4.09, 1H, bd, J=5.0 Hz, NCH 2 3.72, 1H, m, Leul; 3.65, 3H, s, OCH 3 3.52, 1H, bdd, J=10.6, 15.2 Hz, bridge CH 2

CH

2 3.30-3.21, 2H, m's,

CH

2

CH

2 N(b) and PheC'H; 2.94, 1H, bm, PheE(a); 2.76. 1H, bm.

PheO(b); 2.25 water peak; 1.9-1.4, 5H, e, LeuE+O and bridge

CH

2

CH

2 N; 1.29, 3H, d, J=7.1 Hz, Alao; 3.25, 9H, vbr, Boc; 0.92, 6H, d, J=6.2 Hz, LeuD. 13 C NMR (75 MHz, CD 3 CN): E 173.5 (the amide and ester peaks appear to be co-incident); 154.9 (carbamate, br); 140.7; 130.7 129.6; 127.3; 80.54; 66.47; 63.83 62.36; 60.4 (very br); 56.29; 52.97; 44.77; (36.96, 36.40) very br, just resolved; 33.3 (very br); 28.78 (Boc, br); 26.86; 23.90 22.63; 15.47. Mass spectrum (ISMS) m/z 250.2 calculated for C 28

H

37

N

3 0 6 511 fragments (OR 60): 441, 397, (-Boc).

The synthesis of 97 was as for 96 but using the trans amine TLC 1:1 EtOAc:light pet. Rf=0.53. The NMR spectra in CD 3 CN were were well resolved and rotamers were present in the ratio of 11:9; signals attributable to the same atom in the different rotamers are placed in parentheses where possible. 1H NMR (300 MHz, CD 3 CN, ref 1.94 ppm): 0 7.34-7.16, 5H, m; 4.69, 1H, m; 4.13, 1H, d, J=4.4 Hz; 3.92, 1H, m; (3.83, d, J=4.4; 3.79, d, J=4.4 Hz), 1H; 3.76-3.60, 2H, m's; (3.61, s; 3.81, 3H, OCH 3 3.26, 1H, m; 3.15, 1H, m; 2.99, 1H, m; 2.77, 1H, m; 1.85-1.49, 3H, m's; (1.44, s; 1.41, 9H, Boc; 1.30, 3H, d, J=7.2 Hz, AlaO; 1.36-1.24, 2H, m; 0.98-0.91, 6H, m. 13 C NMR (75 MHz, CD 3

CN,

ref 118.69 ppm): 0 174.4; 173.3; 154.6; (140.54, 140.49); 130.7; WO 99/48913 PCT/AU99/0007 130.6; 129.8; 127.6; (80.65, 80.54), Boc tertiary; (66.12, 65.48, 65.21, 64.90) 2xCH; 60.67, CH 2 (56.82, 56.74), CH; (56.41, 56.24), CH; 52.87, CH 3 (46.19, 46.12), CH 2 (40.72, 39.84), CH 2 39.16, CH 2 30.44, CH 2 (29.03, 28.93) Boc; (25.64, 25.58), CH; 24.19, CH 3 22.43,

CH

3 15.76, CH3. Mass Spectrum (ISMS) m/z 488 calculated for

C

2 8

H

37

N

3 0 6 487.

Example Acid catalysed isomerisation of aldehydes 93

CO

2 Bn HCl(cat.), CHCI 3 CO Bn BocN BocN O Bn Bn 93 98 The trans aldehyde was obtained by the acid catalysed isomerisation of the cis diastereomer 93 in chloroform solution with catalytic HCI present. Significant decomposition to multiple unidentified by-products (most having high Rf) also occurs under the isomerisation conditions. The product was purified by flash chromatography eluting with 15% ethyl acetate in petroleum ether for a yield of about 35% 98 from crude 93. 1 H NMR (300 MHz, CD3CN, ref.

1.94 ppm): E 9.41, t, J=1.8 Hz; 7.45-7.10, 10H, m; 5.12, 2H, m,

OCH

2 Ph; 4.46, 1H, br; 4.01, 1H, bd; 3.82, 1H, m; 3.62-3.46, 2H, m; 2.95, 1H, bdd, J=13.0, 4.4 Hz; 2.81, 1H, dd, J=13.2, 8.0 Hz; 2.37, 2H, m (ABq of dd, JAB= 3 1 JddA= 4 6 1.8 Hz; JddB= 7 2 2.1 Hz), O-aldehyde; 1.75-1.25, 12H, e bs, Boc); 0.9, 6H, bm. 13C NMR (75 MHz, CDCl3): 0 202.9; 174.5; 154.4; 139.7; 137.5; 131.0; 129.9; 129.8; 129.7; 127.7; 80.81; 67.61; 64.03 63.18 60.49; 59.9 47.0 45.95; 39.88; 28.96 (Boc); 26.12; 23.25; 22.97.

Example Synthesis of a O-turn mimetic Il(i) WO 99/48913 PCT/AU99/00207 NHBoc 0

H

2 N C0 2 Me

THF

NHBoc

ON

0 99 N NHFmoc

H

2 N CO 2 Bn 101 NaBH(OAc) 3, HOAc, DCE NHBoc NHCbz

N

HN 7 SC02Bn C02Me (C H 2 4 NHFmoc 102 NHCbz (CbzGI) 20 NHBoc

N

CO

2 Me O 100 CO 2 Me FmocHN(CH 2 4 0 NHCbz CO2Bn

CH

2 0, /N_0

O

THF N\ N SBocNj Me0 2

C

103 CbzHN(CH 2 4

O

1. H 2 /Pd-C, MeOH N O 2. TBAF/THF MeN r-N 3. Cbz-CI BocHN X Me0 2

C

105 FmocHN(CH 2 4 0 1. H 2 /Pd-C, N EtOH, HCI 2. BOP, THF, N DIPEA BocN MeO 2

C

Compound 70 was prepared as described above, and reacted with alanine methyl ester to form 99 using the same method previously described for the synthesis of 71. The crude amino ketone 99 (1.22g) was reacted with Cbz-glycine symmetric anhydride (synthesised from 1.95g CbzGlyOH and 9.3mls 0.5M dicyclohexylcarbodiimide in dichloromethane) and 0.6g DIEA in dichloromethane. The reaction was stirred at room temperature for 10 hours then diluted with ether (any DCU precipitate was filtered off) and the ether solution was washed with 1M HCI, aqueous sodium bicarbonate then brine and then dried over magnesium sulfate (removed by filtration) and the volatiles removed under reduced pressure to leave the crude product as an oil which was purified by flash chromatography eluting with 2:1 ethyl acetate:light petroleum ether, yield of 100 was 1.8g Reductive amination of 100 with 101 derived from the deprotection of BocLys(Fmoc)OBn (TFA,

CH

2

CI

2 is carried out by the previously described method for the formation of 73 (71% yield after flash chromatography eluting with 2:1 to WO 99/48913 PCT/AU99/00207 62 3:1 ethyl acetate:light petroleum). The product amine 102 was dissolved in ethyl acetate and formalin added to the stirred solution resulting in the formation of imidazolidine 103. The ethyl acetate solution was washed with aqueous sodium bicarbonate, water (twice), brine and then dried over magnesium sulfate (removed by filtration) and the volatiles removed under reduced pressure to leave the crude product as an oil which was purified by flash chromatography eluting with 3:2 ethyl acetate:light petroleum ether (yield The protected pre-cyclisation compound 103 (400 mgs) was dissolved in 0.1M ethanolic HCI (20 mis) and hydrogenated with 250mgs of 10% Pd-C. The hydrogenation was complete after 7 hours (about 40 psi H 2 room temperature). The solution was filtered through a celite pad to remove the catalyst and 50 mis of DMF added. Volatiles (ethanol) were removed under reduced pressure then a solution of BOP reagent (300 mgs) and DIEA (300 mgs) in 150 mis of DMF was added and the mixture stirred at room temperature for minutes. Most of the DMF was removed under reduced pressure and the residue dissolved in ethyl acetate and washed with 1M HCI, aqueous sodium bicarbonate, water (twice), brine and then dried over magnesium sulfate (removed by filtration) and the volatiles removed under reduced pressure to leave about 300 mgs of crude product 104. The crude product was dissolved in 30 mls methanolic HCI (0.1M) and hydrogenated (200mgs Pd-C, 40psi H 2 for 24 hours reducing the imidazolidine to an Nmethyl group. The catalyst was filtered off (celite) and the solvent removed under reduced pressure, the residue was then treated with tetrabutylammonium fluoride in THF to remove the FMOC group. The free amine was then reprotected by addition of benzyl chloroformate mgs) and DIEA (100 mgs). After stirring for 1 hour ethyl acetate was added and the organic layer was washed with 1M HCI, water, then brine, dried over magnesium sulfate (removed by filtration) and the volatiles removed under reduced pressure to leave an oil which was purified by flash chromatography eluting with 3-5% ethanol in chloroform for a yield of about 40% of 105 based on 103.

WO 99/48913 PCT/AU99/00207 63

APPENDIX

Previous reports of the D-turn mimetic system I(i) A theoretical study of the suitability of various heterocyclic systems as o-turn mimetics has been published (Alkorta et al., 1996).

The study included the 1,3,5-substituted-1,4-diaza-2-oxocycloheptane system (the basis of the o-turn mimetics described herein). No synthesis was described or referenced in the paper for this mimetic system, in contrast to other known mimetic systems where the synthesis was referenced.

Although a search of the Chemical Abstracts registry file on the substructure of the o-turn system gave only the above modelling study, we are aware of a reported synthesis of the n-turn mimetic system by a different synthetic approach. The alternative approach was described in a poster presented at the 23rd European Peptide Symposium (1994), and repeated at the end of a review published in the Bulletin of the Chemical Society of Belgium (Guilbourdenche et al., 1994) and again the following year (Ma et al., 1995). Our research and other literature results do not support this alternative method, the reports are in error and do not represent a reduction to practice. We have repeated the cyclisation reaction described by Ma et al., 1995 and confirmed by NMR analysis and chemical transformation that the actual product is a structural isomer, not the O-turn mimetic claimed. The synthesis and analyses and other material in support of the assertion that the method of Ma et al. does not represent a reduction to practice are presented below.

WO 99/48913 WO 9948913PCT/AU99/00207

R

0 BocHN

"H

R=CH(CH 3 )CH 2 CH 3

R

W'ittig BocHN BnNHOH, R (HCHO) 3 0 1b. BocHNJ ON-Bf

C

6

H

6 reflux

H

2 /Pd(OH) 2

R

EtOH, HCI BocHN JY ___NH 2

OH

CH 0C0 2 Et NaBH 3

CN

R

CO

2 Et BocHN

NH

OH

ZAIaOH, DCC R ZHN 0 Ph 3 P, DEAD CH 2 CI 2 N THF, 200C, 60h BocHN 4% OHAl

CO

2 Et 0

N

RY,

NHBoc Co 2 Et Scheme Al Synthesis proposed by Ma et al., 1995 for a 1 ,4-diazepine []-turn mimetic.

The key step in the proposed synthesis of Ma et al, 1995 is the cyclisation of Al to the protected target A2 using the Mitsunobu reagents. We repeated the synthesis of the cyclisation precursor by our own methods as described below.

The alcohol Al was more conveniently prepared by the conjugate addition method described earlier than as illustrated in Scheme Al (4 steps vs. 6 steps). The procedure used is summarised in Scheme A2.

R OMe BocHN N Me 0 R=CH(CH 3 )CH 2 CH 3 a MgBr R H2N C0E R Et0 2 C lb BocHNI lb. B Bo cH N_ N H THF, r.t. 0 E(OH 0 ZAIaOH, HBTU DIPEA, CH 2

CI

2 ZHN'1 0 NaH4ZHN 0Y BocHN V :N EtOH BocHN 0 A5 C0 2 Et HAl C0 2 Et Scheme A2 WO 99/48913 PCT/AU99/00207 Thus the Weinreb amide of Boc isoleucine was reacted with vinyl Grignard in THF to give the D0- unsaturated ketone A3 by the following procedure: Boc-isoleucine-N-methoxy-N-methylamide (2.25 g, 8.2 mmol) was dissolved in anhydrous THF (20 mL) and cooled to 0 C under nitrogen. To the stirred solution was added vinyl magnesium bromide in THF (20 mL of a ~1M solution) over 5 min. The reaction was very slow at 0°C (negligible progress over 1 but much faster at room temperature product after 20 min). After stirring at room temperature for 90 min the reaction was poured into crushed ice/1M HCI and extracted with ether. The organic layer was washed with 0.5M HCI, water, aq.NaHCO 3 then brine and then dried over MgSO 4 The crude product was formed in good yield and purity and was used directly for the next reaction. TLC 25%EA/light pet. Rf=0.64. 1 H NMR (300 MHz,

CDCI

3 E3 6.50, 1H, dd, J 10, 17 Hz; 6.37, 1H, dd, J 1, 17 Hz; 5.85, 1H, d, J 10 Hz; 5.23, 1H, bd, J 7 Hz; 4.58, 1H, dd, J 4, 8 Hz; 1.88, 1H, m; 1.45, 9H, s; 1.32, 1H, m; 1.10, 1H, m; 0.98, 3H, d, J 7 Hz; 0.90, 3H, d, J 7 Hz. 13 C NMR (75 MHz, CDCl3): 0 199.0; 155.7; 134.0; 129.6; 79.60; 61.71; 37.50; 28.28 (Boc); 24.09; 16.04; 11.61.

Reaction of A3 with glycine ethyl ester in ethanol to give A4 by the following procedure: Glycine ethyl ester hydrochloride (1.0 g, 7.1 mmol) was reacted with A3 (1.1 g, -4.7 mmol) and DIEA (450 mg, mmol) in ethanol (20 mL) at room temperature overnight. The reaction was diluted with ether (100 mL) and extracted in turn with aq. NaHCO 3 and water Petroleum ether was added (100 mL) and the solution extracted with 0.5M HCI:MeOH 4:1 (x3) (discard the organic layer). The acid washings were immediately neutralised with solid NaHCO 3 and then extracted with ethyl acetate and the ethyl acetate layer washed with water then brine and then dried over MgSO 4 Evaporation of the solvent in vacuo left 800 mg of crude product of sufficient purity for use in the next reaction. TLC EtOAc Rf=0.52. 13 C NMR (75 MHz, CDCI 3 i 209.0; 171.7; 155.8; 79.57; 63.95; 60.76; 50.67; 43.69; 40.82; WO 99/48913 PCT/AU99/00207 66 36.74; 28.19 (Boc); 24.05; 16.01; 14.08; 11.51. Mass Spectrum (ISMS) m/z 345 (MH calculated for C 1 7

H

32

N

2 0 5 344.

The amino ketone A4 (690 mg, 2 mmol) was then coupled with Z-alanine to give A5 using standard solution phase coupling procedure with HBTU reagent and DIEA in CH 2 CI2/THF. The crude product was purified by flash chromatography eluting with 30% EtOAc in light petroleum for a yield of 94% (1.03 TLC EtOAc:light pet. 1:2 Rf=0.25. 1 H NMR (300 MHz, CDCI 3 E 7.34, 5H, m; 5.68, 1H, bm; 5.18-5.02, 3H, m's; 4.72, 0.5H, m; 4.48-4.07, 5H, m's; 3.88-3.54, m's; 2.75-2.05, 2H, m's; 1.89, 1H bs; 1.44, 1.43: 9H, 2s, Boc; 1.38, d, J 6.9 Hz (alaHO, one rotamer); 1.34-1.28, 5.5H, m's; 1.07, 1H, m; 1.00-0.82, 6H, m's. 13C NMR (75 MHz, CDCI 3 signals due to the equivalent carbon in different rotamers are grouped in parentheses where possible: 0 (209.0, 207.9); (173.39, 173.25); (169.15, 168.84); 155.75, 155.67, 155.56, 155.33: carbamate signals; 136.20; 128.31; 127.91; 127.80; (79.72, 79.57); 66.60; (64.01, 63.85); (61.61, 61.09); (50.96, 48.65); (46.63, 46.57); (43.75, 43.23); (40.02, 39.07); (36.56, 36.29); 28.14 (Boc); (24.09, 24.03); 18.74; 15.92; 13.85; (11.44, 11.38). Mass Spectrum (ISMS) m/z 550 calculated for C 2 8

H

43

N

3 0 8 549 The ketone A5 (430 mg, 0.78 mmol) was dissolved in ethanol (5 mL) and NaBH 4 (15 mg, 0.40 mmol) added to the stirred solution at room temperature, and stirring continued for 1 h. The solvent was removed in vacuo and the residue dissolved in ethyl acetate and washed with 1M HCI, water, aq. NaHCO 3 brine and then dried over MgSO 4 The residue after solvent evaporation was purified by flash chromatography eluting with ethyl acetate:light petroleum ~1:1 (some separation of diastereomers occurred) for an approximately quantitative yield of the alcohol Al. TLC EtOAc:light pet. 1:1 Rf=0.28. 1 H NMR (300 MHz, CDCl3), late eluting fractions, rotamers/diastereomers 0 7.39- 7.29, 5H, m; 5.80, 1H, d, J=9 Hz; 5.15, 1H, d, J=12 Hz; 5.11-5.49, -1H, m; 4.96, -1H, d, J=12 Hz; 4.67-4.42, ~1H, m's; 4.19, -2H, bq, J=7.2 Hz; 4.03-3.88, ~2H, bm; 3.88-3.40, ~4H, m's; 3.30-3.09, 1H, m; 1.96-1.66, WO 99/48913 PCT/AU99/00207 67 -2H, m; 1.55, -1H, m; 1.42, 9H, s, (Boc); 1.331.33, d, J=7 Hz; 1.28, t, J=7.2 Hz; 1.15, d (minor isomer), J=6.8 Hz; 1.37-1.05 -8H; 1.0-0.82, ~6H, m's. 13 C NMR (75 MHz, CDCl3), major peak only shown unless otherwise indicated: E 174.0; 169.0; 156.4; 156.3; 135.9; 128.4; 128.1; (128.0, minor isomer); 127.9; 78.92; 66.96; (66.56, minor isomer); 66.11; 61.26; 59.49; 47.74; 46.10; 45.24; 34.38; 31.31; 28.30 (Boc); 22.29; 18.85; 16.41; 14.00; 11.90. Mass Spectrum (ISMS) m/z 552 (M+H calculated for C 28

H

45

N

3 0 8 551.

The alcohol Al was reacted with the Mitsunobu reagents as described by Ma et al., 1995 (Scheme 4.37) as follows: The alcohol Al (150 mg, early eluting fraction) was dissolved in dry THF and triphenylphosphine (71 mg) added. To the stirred solution at room temperature under nitrogen was added DEAD (43 uL), and stirring continued for 24 h. Analysis of the crude reaction revealed the formation of a dehydration product (M+H+=534 Da) in moderate yield. Another equivalent of triphenylphosphine/DEAD was added and stirring continued for a further 48 h. The solvent was removed in vacuo and the residual oil dissolved in ether/petroleum ether and left to stand to encourage the precipitation of the triphenylphosphine oxide and diethoxycarbonyl hydrazine (white solid, filtered off). The oil remaining after evaporation of the filtrate was purified by flash chromatography eluting with petroleum ether and 10-100% ether in petroleum ether, yield was ~40% (60 mg).

TLC ethyl ether Rf=0.61. The NMR spectra were quite complex, as may be expected from the possible mixture of diastereomers/ rotamers.

However, it was possible to clearly identify the alanine spin system with HO at 4.71 ppm (1H, broad pentuplet, J~8Hz). 1D decoupling experiments were performed: irradiation at 4.7 ppm caused the collapse of two signals to singlets, a doublet centred on 1.40 ppm (J=7Hz, alanine HO), and a broad doublet (1H, J=8Hz) at 5.62ppm (alanine NH). These assignments were confirmed by irradiation at 1.4 ppm which caused collapse of the multiplet at 4.71 ppm to a doublet with J=8Hz. The presence of the NH proton in the alanine spin system rules out the O-turn WO 99/48913 PCT/AU99/00207 68 mimetic A2 proposed by Ma et al., 1995 as a possible structure for the product, and leaves open the possibility of A6 or A7 (Scheme A3) which we felt were more probable products, as the true structure. 1 H NMR (300 MHz, CDCl3): (selected peaks) O 5.62, -1H, bd, J=8 Hz; 4.71, -1H, 1.40, d, J=6.8 Hz. Decoupling experiments: irradiate 1.4 ppm 4.71 doublet, J=8 Hz; irradiate 4.71 ppm 1.4 singlet, 5.62 singlet.

13 C NMR (75 MHz, CDCI 3 the spectra were difficult to analyse due to the presence of rotamers/diastereomers, peak broadening and impurities which co-eluted. There were a couple of notable features: the appearance of a new peak at the relatively unusual shift of 160.7 ppm possibly due to the carbamate derived oxazoline carbon (only one carbamate resonance was observed, 155.5 ppm), and (ii) the downfield shift of the tertiary Boc carbon resonance which was observed at 81.22 ppm, whereas NHBoc tertiary carbon shifts are normally at a shift upfield of 80 ppm 78.9 in the alcohol precursor). Mass Spectrum (ISMS) m/z 534 calculated for C 28

H

43

N

3 0 7 533.

To confirm the results of the NMR analysis a further experiment was carried out. The product material was hydrogenated (EtOH, Pd-C) to remove the Z group. If the product has structure A6 or A7 then the amine will now be free to form the diketopiperazine A8, a facile reaction in such a system, Scheme A3. If any of the target O-turn mimetic A2 is present then it will be deprotected to the (very stable) free amine A9 and be easily detected in the ionspray mass spectrum (ISMS).

Analysis of the product mixture from the hydrogenation revealed the presence of a mass peak corresponding to the diketopiperazine (MH'=354Da), but no trace whatsoever of A9 (MH =400Da).

WO 99/48913 PCT/AU99/00207 R ZHN 0 BocHN

N

OH

C0 2 Et PhP 3

,DEAD

Z HN 0 BocN C02Et PhP 3

DEAD

ZHN 0 ZN O H 2 /Pd-C C0 2 Et O °NH2 O -EtOH

CO

2 Et 01 O-N NH

,FL<AO

A8 MH =354Da

H

2 0 MH +=552Da NHBoc C02Et alcohol) A9 MH =400Da Scheme A3 Finally, it was also observed that the cyclisation product (which we propose to be A6) was easily hydrolysed by dilute aqueous acid room temperature 0.1% aq. TFA, 12 back to the alcohol Al (or a compound of the same mass). This last observation is more consistent with the product structure being the oxazoline A6 rather than the aziridine A7 as the oxazoline is more probably subject to facile hydrolysis by aqueous acid, the facile hydrolysis is entirely inconsistent with the structure A2 proposed by Ma et al., 1995 In further support of A6 as the product structure, peptide alcohols similar in structure to Al have been reported to form oxazolines, (GalEotti et al., 1992) for example: 0 Ph 3

P,

OH 0 DEAD, No H THF BocNH 1 Val-ValOMe----T

I

9

N

BocNH Val-ValOMe 58% yield 0 WO 99/48913 PCT/AU99/00207 Other evidence against formation of A2 by the Mitsunobu reaction as proposed by Ma et al., 1995 is presented below.

Difficulty of forming seven membered rings via the Mitsunobu reaction Literature precedent The literature on the formation of cyclic amines and amides with the Mitsunobu reaction contains numerous examples of the formation of 3-6 membered rings (Carlock and Mack, 1978; Robinson et a/.,1983; Pfister, 1984; Kelly et al., 1986; Henry et al., 1989; Bernotas and Cube, 1991), but very few cases of seven membered ring formation. In one paper on the cyclisation of amino alcohols the faliure to form a simple seven membered target is specifically described (Bernotas and Cube, 1991) In the organic reactions entry on the Mitsunobu reaction (Hughes, 1992) three instances of seven membered ring formation with carbonnitrogen bond formation are described: all three involve a primary alcohol, two occur in polycyclic systems and appear to be special cases, and the third involves alkylation of a hydroxamide far easier than an amide due to higher NH acidity.

There appears to be no literature precedent for the formation of a seven membered ring to a simple amide or carbamate nitrogen. In addition there is little precedent for secondary amide Nalkylation with hindered secondary alcohols, as is proposed to occur in the formation of A2.

Synthetic studies Extensive studies on the use of the Mitsunobu reaction for the formation of the target system were carried out in our laboratories prior to becoming aware of the proposed synthesis. In our hands this approach was ineffective. The key reactions are described in Schemes A4 and WO 99/48913 PCT/AU99/00207 Ph FA Ph 3

P,DEAD

F

3 C N CO 2 Me THF H OH

OH

OH 0 S N MCOMe PhB O Bn

'COCF

3 O Ph F3C N CO 2 Me Scheme A4

H

EtOC. .N.

2N CO Et 18% EAD, Ph 3 P. THF EAD, Ph 3 P THF Ph

FO

3 C Oj 0 -N0 Ph TfN- 0

N

NBoc 2 I MeO 2 Bn All Scheme The formation of the alkylation product was somewhat successful in the intermolecular reaction (Scheme A4), but this success was not repeated in cyclic systems (Scheme A5). No significant amount of the target cyclic products A10 or All was detected.

Competing reactions oxazoline and aziridine formation Cyclisation of O-hydroxy amide derivatives A12 with the aim of forming D-lactams A13 also results in the formation of the aziridine A14 and oxazoline A15 products shown in Scheme A6 (Hughes, 1992).

Another example of oxazoline formation was described above (Galeotti et al., 1992).

WO 99/48913 PCT/AU99/00207 72

R

RCONH R' O 0 N o o

R

Co 0C 0

R

ZNH NHCR A13 NHZ Ph 3 P,DEAD COR R' OH

N

A12

,R'

O:HZ A14

NHZ

Scheme A6 As the Mitsunobu reaction is relatively effective for the formation of small ring sizes, it is quite probable that the formation of aziridines and oxazolines will compete with other possible cyclisations, other factors being equal. Such competition can take place in the proposed synthesis, the products would then be A6 and/or A7, Scheme A3. Both the aziridine and oxazoline are isomeric with the target compound A2, possibly leading to their confusion with the target, a situation easily resolved by 1 H NMR as we demonstrated above.

In summary, the proposed method is in error because: We have repeated the cyclisation and found the product to be a structural isomer of the target, probably the oxazoline A6.

This finding is supported by:- Literature contrindications (competing cyclisations favoured), lack of precedent (seven membered rings difficult to form by the Mitsunobu reaction).

Extensive studies in our laboratories which indicate the Mitsunobu approach is generally ineffective for the synthesis of the 0-turn mimetics.

WO 99/48913 PCT/AU99/00207 73

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WO 99/48913 WO 9948913PCT/AU99/00207 SCHEME 1

/NH

pgN H2NkIG

CH

2

CI

2

-H

2 0 N kG §N N 3a-d 2a-d

M

M' Rgl

B-

Iaq. CH 2

O,

dihydroxylt oxidise, e.g.

5a-d 1uC 3 Na1O 4 lozonolysis pgN..I oxidative cleavage

R

2 -N G

R

1 M' M" oxidise 7a-d Sreduce or reduce, oxidise 6a-d 8a-d Scheme 1 WO 99/48913 WO 9948913PCT/AU99/00207 SCHEMES 2 AND 3 PgUpgC

R

M HN-ypc 0 reductive anination pgC A R 3 'N pgC.

H

0 M, Mil Scheme 2 pgC M R3 N pgC 0 deprotect PgC PgtL Pg

C

cyclise (coupling agent) pgN -N H 13 deprotect imidazolidine gC, Pg H' aq. for Z=-H hydrogenate for Z--Me Scheme 3. Synthesis of y-tum mimetics I(i).

WO 99/48913 PCT/AU99/00207 79 SCHEME 4 2pgN'

R

1 M'M1"H 6b 100), Z=-H deroectI imidazolidine

R

2 INpgC P gN- N> R PNy.C02HR couple '1 FAA.

Scheme 4. Synthesis of 'y-turn mimnetics I(R).

WO 99/48913 PCT/AU99/00207 SCHEMES 5 AND 6 Pg 0

C

H

F

pgN' 0 acid fluoride (or other suitable coupling method) 0 Pg 0

P

base, solvent Pg 0 P r~ pgN

I

16 'PgC Deprotect pN' pC I (acid conditions) N 0 R MeN H R1 N Pg 0 11(i) I1. cyclise (couple) 2. hydrogenate irnidazolidine 17 1Pg 0 Scheme 5. Synthesis of of 1-turn mimetics II(i).

Pg 0 H 0 PgNdeprotect Pg

N

ZN

H

R 1 N HN :CR 4 HI~ M, 0 0 C PgN Pg deprotect imidazolidine PgN- Scheme 6. Synthesis of j3-turn mimetics 11(ui).

WO 99/48913 WO 9948913PCT/A U99/00207 81 SCHEMES 7 AND 8 Rj 2 PgN-N 0 ,CO 2

H

r 1 M. Mfg 6a deprotect im-idazolidine

F-

PgN\

R

HN

20 -gC pgC' couple couple: BOP, THF, DEPEA deprotect PgN, pgC' 2 0 RNr-4

H

2

N

r~R3 pgC Scheme 7. Alternative synthesis of beta turn mimnetics 11(ii)

R

2

BH

hydroborate R2 pgL -NG PgN4 M 24a-d

-BR

2 oxidise PgRN H2IYPgC, 0 reductive 9 amination Scheme 8. General methods used in the synthesis of mimnetics 1I(iii) and1(i) WO 99/48913 PTA9/00 PCT/AU99/00207 SCHEMES 9 AND lPg 0 Pg

C

ZN H ,g.NH M' l x g.

Same method as described in Scheme 9. Synthesis of beta turn mimetics II(iii): Scheme 5, substituting 26 for R2

G

PgN-N -I lM

R

1 M' M" C0 2

H

ON-

pgN'c nir '741, II(iv) Scheme 10. Synthesis of beta turn mimetics II(iv): same method as described in Scheme 6, substituting 25c for 6c; alternatively, same method as for Scheme 7, substituting 25a for 6a.

WO 99/48913 WO 9948913PCT/A1J99/00207 SCHEME 11 Deprotect PgN' PgN-- 6pgC 27 N pgC Leetcouple HN C0H (rpeatdeprotect, couple Pg(A)' I0Htms deprotect (ii) cyclise (iii) hydrogenate 11IO), n= 1 Pg 0 Scheme 11. Synthesis of beta buidge mimic III(i) using the general method for the synthesis of 11(i) (as described in Scheme WO 99/48913 WO 9948913PCT/AU99/00207 SCHEME 12 dehydrate Pgm-I-N--jrPg 0

C

PgN~ 0 0

R

1

MM

Solvent (e.g.

EtOH), heat

R

3 pgN<' Nl C 2

H

couple SPg

C

Pg 0 0 oxidiseI

HN

pgN' Pg 0 32 0'

HN

p 9

N'

deprotect PgN' and Pg 0 reductive amnination deprotect Pg 0 couple ZNN

R

4 R M. M1

NH

pgN

IV(,)

Pg 0 Scheme 12. Synthesis of bicyclic f 3 -turn mimetic systems IV(i).

WO 99/48913 WO 9948913PCT/AU99/00207 SCHEME 13

R

2 I-N G or pgN-N

OH

R

1 M' M OH oxidise c 7a, c 35a, c reductive amination deprotect PgN' then couple n y.

IV(ii) P1 )gc deprotect imidazolidine reductive amination deprotect pgN', pgC'; couple Pg 0 -IN 0

R

4 Fpg Scheme 13. Synthesis of bicyclic beta turn mimetic systems IV(ji).

WO 99/48913 WO 9948913PCT/AU99/00207 86 SCHEMES 14 AND R' R COPgC CO9g 0 PgNN *1 gN O H H

H

41 42 Scheme 14. Alkylated aspartic and glutarnic acid derivatives.

F HN

Y

HN N YR 4 pgN I (Z-H) x y 10) CH(M) C(O) 1(u) C(O) CH 2 COPgC H C

H

le See text for methods.

deprotect Pg.

R

4 cyclise deprotect PgC 34 -cycise- I Y 4

RC

final steps: deprotect PgN, alkylate or reductively amninate and/or couple I 4

HN

pgN pgN Scheme 15. Synthetic methods for the neutral bicyclic J0-tum mimetics V and VI.

WO 99/48913 WO 9948913PCT/AU99/00207 SCHEME 16 e

C

2 Me PfHN 00 2 t-Bu Pf phenyifluorenyl

KHMDS

R'l or R'OTf 39 0O 2

H

0 TBS LDA, R'X

CH

2 0TBS 0 TBS LDA, R'X

CH

2 0TBS 0 TBS 00 47 Ph Pg(C)- 0 48 Scheme 16. Alkylation of aspartic acid derivatives WO 99/48913 WO 9948913PCT/AU99/00207 SCHEME 17 KSYCO2tBu MeO 2 CNH'Pf Pf phenyifluorenyl KHMVDS, R'X Me0 2 C C 2 t-Bu R' HPf

N

0 Ph LIDA, R'X o N OTBS I LIDA, R'X Bsoc

R'-

0 Ph a/3 selectivity varies depending on R' o N OTBS Boo I1.LDA, PhSeCI 3.HPd-C o N OTBS Boc 41 42 Scheme 17. Aikylation of glutarnic acid derivatives.

WO 99/48913 WO 9948913PCT/AU99/00207

CH

3

ICH

3 SCHEME 18 Nrr 10'"MgBr THF, 0 0

C.

HW OPgC

THF

H' M OPgC 9 N 51 acylate, CH 2 C1 2 ICbzH rAl(F DIBA01 protect amine Pg N=Cbz 0g

H

PgN CbzH OPg 0 2

HH

H

2 IPd-C O.1M HCI in EtOH Ideprotect PgC 0 pgN' Couple (cyclise);

R

BOP, DMIF/CH 2 C1 2 H 02H DIBA

HH

PgN f*og I(i)a R'=H1-) 1. deprotect PgN' 2. CH 2 O, THF M, M' and M"=H) Scheme 18. Shorter procedure for the preparation of 10 and I(i)a where RI is hydrogen.

Claims (60)

1. A general mimetic of the structure Q 2 Q R 2 R RN M' Q4N M" x wherein:- indicates a bond at a chiral centre of the structure which centre may be in the R or S configuration or a mixture thereof; R and R 2 is an amino acid side chain group which may be the same or different; M' and M" may be the same or different and are selected from the group consisting of hydrogen, C 1 -C 4 alkyl, chloro and C 1 -C 4 alkoxy; RN is -N(Z')PgN where Z' is selected from the group consisting of hydrogen, methyl and part of a cyclic amino acid sidechain joined to Q' and PgN is a protecting group for amine; Rc is selected from the group consisting of a carboxy terminal part of the mimetic, hydrogen, R and -CH 2 R; Q1 R 1 which has the same definition as R and R 2 above and Q 2 Z where Z is selected from the group consisting of hydrogen, methyl, ethyl, formyl and acetyl, -CH 2 R, and -C(O)R or alternatively Z is part of a cyclic amino acid side chain group joined to R 2 or Q1 and Q2 taken together represent a cyclic group; Q 3 is selected from the group consisting of Y. C(O)NHCH(R)Y-, -C(O)ENHCH(R)Y-, -C(O)N(Q 5 )CH(R)Y- wherein Y is selected from the group consisting of C(0) and CH 2 and QS is a covalent bond from the Q 4 group to the nitrogen atom in Q3 to form a bicyclic ring system or alternatively, is selected from the group consisting of hydrogen, WO 99/48913 PCT/AU99/00207 91 C 1 -C 4 alkyl, chloro and C 1 -C 4 alkoxy and E is (AA)n where n is 1-300 and AA is an amino acid residue; and Q 4 is selected from the group consisting of C(O), CH(Q 5 )CH 2 and CH(Q 5 C(O); with the provisos that when:- Q4 Y is C(O); (ii) Q4 Y is CH 2 (iii) Q 4 CH(Q 5 )CH 2 Y is C(O); (iv) Q 4 CH(Q 5 Y is CH 2 Q 3 -C(O)N(Q 5 )CH(R)Y, Q5 is a covalent bond from the Q 4 group to the nitrogen atom in Q3 which is a cyclization forming a bicyclic ring system.

2. A peptide mimetic as claimed in Claim 1 wherein when Q1 and Q 2 form a cyclic group Q 1 Q 2 which is selected from the group consisting of -CH 2 -CH 2 CH 2 CH(R)C(O)-, -CH(R)CH 2 -CH 2 CH(R)CH 2 -CH 2 CH 2 CH(R)CH 2 -CH 2 CH(R)-, CH 2 CH 2 -CH(R)CH 2 CH 2 -CH 2 CH(R)CH 2 CH 2 CH(R)CH 2 and -CH 2 CH(R)CH 2

3. A peptide mimetic as claimed in Claim 1 wherein n is 1-30.

4. A peptide mimetic as claimed in Claim 1 wherein E represents a loop of n amino acids which additionally incorporate non- alpha amino acid(s), alpha dialkyl amino acid(s) or other amino acid which provides the peptide mimetic with increased binding affinity or increased ease of detection, identification or purification.

5. A peptide mimetic as claimed in Claim 1 wherein Q' is R, Q2 is Z, Q3 is Y.

6. A peptide mimetic as claimed in Claim 1 wherein Q 1 is R, Q2 is Z, Q 3 is C(O)NHCH(R)Y and Q 5 is M'.

7. A peptide mimetic as claimed in Claim 1 wherein Q 1 is R, Q 2 is Z, Q3 is C(O)NHCH(R)C(O)-NHCH(R)Y and QS is M'.

8. A peptide mimetic as claimed in Claim 1 wherein Q' is R, Q2 is Z, Q3 is C(O)N(Q 5 )CH(R)Y and Q 5 is a covalent bond to Q3. WO 99/48913 PCT/AU99/00207 92

9. A peptide mimetic as claimed in Claim 1 wherein Q' is CH(R)C(O)Q 2 Q 2 is a covalent bond to Q 1 Q3 is Y and Q 5 is M'. A peptide mimetic as claimed in Claim 1 wherein Q 1 is CH 2 CH(R)C(O)Q 2 Q2 is Q 1 Q3 is Y and Q 5 is M'.

11. A peptide mimetic as claimed in Claim 1 wherein Rc is C(O)Pgc where PgC is a protecting group for carboxylic acid.

12. A peptide mimetic as claimed in Claim 11 wherein PgC is selected from the group consisting of alkoxy, benzyloxy, allyloxy, fluorenyl methyloxy, amines forming easily removable amides, a cleavable linker to a solid support, the solid support, hydroxy or NHR R, C(O)R or the remaining C-terminal portion of the mimetic.

13. A peptide mimetic as claimed in Claim 12 wherein PgC is methoxy or ethoxy.

14. A peptide mimetic as claimed in Claim 1 wherein PgN is a protecting group for an amine. A peptide mimetic as claimed in Claim 1 wherein pgN is selected from the group consisting of Boc, Cbz, Fmoc, Alloc, trityl, a cleavable linker to a solid support, the solid support, hydrogen, R, CO(R) or part of the remaining N terminal portion of the mimetic.

16. A peptide mimetic as claimed in Claim 1 wherein M' or M" is methoxy.

17. A peptide mimetic as claimed in Claim 1 wherein M' or M" is methyl.

18. Compounds I(i)a herein.

19. Compounds I(i)a herein where R, and R 2 H. Compounds I(ii)a herein.

21. Compounds I(ii)a herein where R 1 and R 2 H.

22. Compounds II(i)a herein.

23. Compounds Il(i)a herein where R 1 and R 2 H.

24. Compounds ll(iii)a herein. Compounds Il(iii)a herein where R, and R 2 H.

26. Compounds ll(i)a herein. WO 99/48913 PCT/AU99/00207 93

27. Compounds IIl(iii)a herein.

28. Compounds IV(i)a herein.

29. Compounds IV(ii)a herein. Compounds V(i)a and V(ii)a herein.

31. Compounds Vl(i)a and Vl(ii)a herein.

32. Compounds 4a-d herein.

33. Compounds 5a-d herein.

34. Compounds 6a-d herein. Compounds 7a-d herein.

36. Compounds 8a-d herein.

37. Compounds 4a-d herein where R 1 and R 2 H.

38. Compounds 5a-d herein where R 1 and R 2 H.

39. Compounds 6a-d herein where R 1 and R 2 H. Compounds 7a-d herein where R 1 and R 2 H.

41. Compounds 8a-d herein where R 1 and R 2 H. 42 Compounds 10 herein or compounds 10 where R 1 and R 2 H.

43. Compounds 11-14, 16-19, 21-22, 23(a-d), 25(a-d), 26-34, 36-38 and 43-46 or compounds 11-14, 16-19, 21-22, 23(a-d), 25(a-d), 26-34, 35(a-c), 36-38 and 43-46 where R' and R 2 H.

44. A process for preparation of compounds 4a-d herein comprising the reaction of imines 3a-d herein with an allyl boron reagent to provide compounds 4a-d. A process as claimed in Claim 44 wherein imines 3a-d are prepared by condensation of amino acid aldehydes 1 herein and amines 2a-d herein.

46. A process as claimed in Claim 44 wherein addition of formaldehyde solution to compounds 4a-d provides imidazolidines herein.

47. A process as claimed in Claim 46 wherein compounds 6a-d herein are obtained by oxidation of imidazolidines WO 99/48913 PCT/AU99/00207 94

48. A process as claimed in Claim 46 wherein imidiazolidines are dihydroxylated to provide compounds 7a-d herein.

49. A process as claimed in Claim 46 wherein aldehydes 8a-d herein are obtained by ozonlysis of imidazolidines

50. A process as claimed in Claim 48 wherein aldehydes 8a-d are obtained by oxidation of compounds 7a-d.

51. A process as claimed in Claim 48 wherein compounds 6a-d are reduced to form aldehydes 8a-d.

52. A process as claimed in Claim 50 wherein aldehydes 8a-d are oxidized to provide carboxylic acids 6a-d.

53. A process as claimed in Claim 50 wherein aldehydes 8a are subjected to reductive amination with compound 9 herein to provide amines 10 herein.

54. A process as claimed in Claim 53 wherein amines 10 are subjected to removal of group PgC' to provide compounds 11 herein. A process as claimed in Claim 54 wherein compounds 11 are subjected to cyclization to provide compounds 12 herein.

56. A process as claimed in Claim 55 wherein mimetics I(i) herein are obtained by hydrogenation of compounds 12.

57. A process as claimed in Claim 55 wherein mimetics I(i)a herein are produced by acid hydrolysis of compounds 12.

58. A process as claimed in Claim 47 wherein mimetics I(ii) are obtained by:- removal of group PgA' from compounds 6b to provide compounds 13 herein; (ii) cyclization of compounds 13 to provide compounds 14 herein; and (iii) deprotection of the imidazolidine group in compounds 14.

59. A process as claimed in Claim 53 wherein amines 10 are reacted with compounds 15 herein in the presence of base to provide compounds 16 herein, whereby groups PgN' and PgC' are subsequently WO 99/48913 PCT/AU99/00207 removed to provide compounds 17 herein which, after hydrogenation and cyclization, provide mimetics II(i) herein. A process as claimed in Claim 47 wherein compounds 6c have the group PgN' removed to provide compounds 18 herein which are converted to compounds 19 herein which by deprotection of the imidazolidine group are converted to mimetics II(ii) herein. 61 A process as claimed in Claim 47 wherein compounds 6a are reacted with compound 20 herein to provide compound 21 herein which, after removal of groups PgN' and PgC' are converted to compounds 22 herein which are subsequently converted to compounds 19 which by deprotection of the imidazolidine group, are converted to mimetics Il(ii) herein.

62. A process as claimed in Claim 46 wherein compounds are converted to compounds 23a-d herein by hydroboration whereafter compounds 23a-d are oxidized to compounds 24a-d herein whereafter compound 24a is subjected to reductive amination with compound 9 to provide compounds 26 herein which are subsequently converted to mimetics Il(iii) herein.

63. A process as claimed in Claim 46 wherein compounds are converted to compounds 23a-d herein by hydroboration whereafter compounds 23a-d are oxidized to form compounds 25a-d herein and subsequently compound 25a or 25c is converted to mimetics Il(iv) herein.

64. A process as claimed in Claim 53 wherein amines 10 are reacted with compounds 15 herein which compounds in the presence of base are converted to compounds 16 herein which then have the group PgN removed to provide compounds 27 herein which after reaction with compound PgN'NHCH(R)COOH are converted to compounds 28 herein which are subsequently converted to mimetics ll1(i) herein. A process as claimed in Claim 48 wherein compound 7a is dehydrated to provide compound 29 herein which are then converted to compound 30 herein whereafter compounds 30 by reaction with compound PgN'NHCH(R)COOH form compounds 31 which are then WO 99/48913 PCT/AU99/00207 96 oxidized to form compounds 32 herein which after removal of groups PgN' and PgC' and reductive animation are converted to compounds 33 herein which are subsequently converted to compounds 34 herein which after deprotection of the imidazolidine group is converted to mimetics IV(i) herein.

66. A process as claimed in Claim 46 or 48 wherein compounds c or 7a, c are oxidized to form compounds 35a, c herein whereafter compounds 35c are subjected to reductive animation to form compounds 36 herein which after removal of the group PgN' are converted to compounds 37 herein whereafter mimetics IV(ii) are produced by deprotection of the imidazolidine group.

67. A process as claimed in Claim 46 or 48 wherein compounds c or 7a, c are oxidized to form compounds 35a, c herein whereafter compounds 35c are reacted with compounds 26 herein to form compounds 38 which after removal of the groups PgN' and PgC' are converted to compounds 37 which after deprotection of the imidazolidine are converted to mimetics IV(ii).

68. A process as claimed in Claim 57 wherein mimetics I(i) wherein R' is an alkylated aspartate or alkylated glutamate side chain which correspond to compounds 43 and 45 respectively which subsequently each have the group PgC' removed and cyclized to provide compounds 44 and 46 respectively which are subsequently converted to mimetics V and VI respectively.

69. A process of making compounds 54 herein wherein initially compounds 49 herein are converted to compounds 50 herein which thereafter after reaction with compounds 9 herein produces compounds 51 herein which are subsequently converted to compound 52 herein which are then reductively aminated with compounds 9 to provide said compounds 54.

70. A process as claimed in Claim 69 wherein compounds 54 are converted to compounds 55 after removal of groups PgC' and PgN' which are then converted to mimetics I(i)a where Z and R' is H. WO 99/48913 PCT/AU99/00207 97

71. A process as claimed in Claim 69 wherein compound 54 after removal of PgN' is converted to compounds 10 herein wherein M, M' and M" are H.

72. A process for making mimetics I(i)a herein stereospecifically wherein compounds 49 herein are reacted with vinyl magnesium bromide to compounds 50 herein which are then reacted with compounds 9 herein to form compounds 51 herein which are then reacted with compounds herein wherein PgN' is Cbz to form compounds 53 herein which are then converted to mimetics I(i)a by hydrogenation.

73. A library of peptide mimetics comprising at least one mimetic from any one of Claims 1-31.