AU660930B2 - (S)(+)-2-ethoxy-4-(N-(1-(2-piperidinophenyl)-3-methyl-1- butyl)aminocarbonylmethyl)benzoic acid - Google Patents
(S)(+)-2-ethoxy-4-(N-(1-(2-piperidinophenyl)-3-methyl-1- butyl)aminocarbonylmethyl)benzoic acid Download PDFInfo
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- C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
- C07D295/04—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms
- C07D295/12—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly or doubly bound nitrogen atoms
- C07D295/135—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly or doubly bound nitrogen atoms with the ring nitrogen atoms and the substituent nitrogen atoms separated by carbocyclic rings or by carbon chains interrupted by carbocyclic rings
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Description
OPI DATE 25/01/93 AOJP DATE 25/03/93 PCT NUMBER PCT/EP91/01147I1111liiIDii ii1111 i AU91 80781 ""mIv jzuvL. i "LL. r,~inL~iN i vy no nvra krt1) (51) Internationale Paten tklassifi kation 5 Internationale Verdffentlichungsnummer: WO 93/00337 C07D 295/12, A61K 31/445 Al (43) Internationales Veriiffcntlichungsdatum: 7. .lanuar 1993 (07.01.93) (21) Internationales Aktenzeichen: PCT/EP91/0l 147 (74)GemeinsamerVertreter: DR. KARL THOMAE GMBH; Patentstelle, Postfach 17 55, D-7950 Biberach/RiR (DE).
(22) Internationales Anmeldedatum: 21, Juni 1991 (21.06.91) (81) J1estimmungsstaaten: AT, AU, BB, BG, BR, CA, CH, CS, (71) Anmelder: DR. KARL THOMAE GMBH [DE/DE]; Post- DE, DK, ES, Fl, GB, HU, JP, KP, KR, LK, LU, MC, fach 17 55, D-7950 Biberach/Ri MG, MW, NL, NO, PL, RO, SD, SE, SU, europtlisches Patent (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, (72) Erfinder: GRELL, Wolfgang Greschwister-Scholl-Strage NI, SE), OAPI Patent (BF, BJ, CF, CG, CI, CM, GA, 18, D-7950 Biberach I GREISCREL, Andreas GN, ML, MR, SN, TD, TG).
Nensatzweg 27, D-7950 Biberach I ZAHN, Gabriele Schlierenbachstrage 14, D-7950 Biberach (DE).
MARK, Michael Schlehenhang 17, D-7950 Biberach Ver6ffentlicht KNORR, Hansj~rg Waldstrage 39, D-6507 Ingel- Mit internationalein Recherchenberichi.
heim RUPPREGHT, Eckhard Riedbachstrage D-7960 Aulendorf-Tannhausen MOLLER, CIIrich Bfirgerturmstrage 1, D-7950 Biberach (DE).
(54) Title: +)-2-ETHOXY-4-N-E11-(2-PIPERIDINOPHENYL)-3-METHYL-1I-BUTYL]AMINOCARBONYLMET- HYL]BENZOIC ACID (54) Bezeichnung: +)-2-ATHOXY-4-[N-11 -(2-PIPERIDINO-PH ENYL)-3-METHYL-1I-BUTYL)AMINOCARBONYL-
METHYL]-BENZOESAURE
(57) Abstract The invention concerns the new(s)( +)-2-ethoxy-4-[N- I -(2-pipericinophenyl)-3-methyl- I-butyllaminocarbonylmethyllbenzoic acid and its salts, which have useful pharmacological properties, producing an action on the intermediate metabolism and in particular a lowering of blood-sugar levels. The invention also concerns the new intermediates of the formulae (I11), (IV) and (VII) as well as their addition salts. The new compounds can be prepared by prior art methods used to prepare analogous compounds.
(57) Zusammenfassung Die vorliegende Anmeldung betrifft die neue +)-2-Athoxy-4-[N-[ l-(2-piperidino-phenyl)-3-methyl- I-butyljaminocarbonyl methyl]-benzoesflu re und deren Salze, welche wertvolle pharmakologische Eigenschaften aufweisen, niimlich eine Wirkung auf den Intermedifirstoffwechsel, insbesondere jedoch eine blutzuckersenkende Wirkung. Emn weiterer Gegenstand sind die neuen Zwischenprodukte der Formeln (111), (IV) und (VII) sowie deren Additionssalze. Die neuen Verbindungen lassen sich nach fOr analoge Verbindungen bekannten Verfahren herstellen.
S- 1 56830J.21 DR. KARL THOMAE GMBH Case 5/1072-FL D-7950 Biberach/Rip (S)(+)-2-Ethoxy-4-[N-[1-(2-piperidino-phenyl)-3-methyll-butyl]aminocarbonylmethyl]-benzoic acid, pharmaceutical compositions containing this compound and processes for the preparation thereof EP-B-0147850 describes inter alia the racemate of 2ethoxy-4-[N-[l-(2-piperidino-phenyl)-3-methyl-l-butyl]aminocarbonylmethyl]-benzoic acid (Code No.: AG-EE 388 ZW) of the formula ch
CH
CH
C 0 OO
H
NH OC2H
S
and EP-B-0207331 describes two other polymorphous forms of this compound. This compound and the physiologically acceptable salts thereof have valuable pharmacological properties, namely an effect on the intermediate metabolism, but more particularly the effect of lowering blood sugar.
The two enantiomers of this compound, namely ethoxy-4-[N-[l-(2-piperidino-phenyl)-3-methyl-l-butyl]aminocarbonylmethyl]-benzoic acid (Code No.: AG-EE 623 ZW) and (R)(-)-2-ethoxy-4-[N-[l-(2-piperidinophenyl)-3-methyl-l-butyl]aminocarbonylmethyl]benzoic Sacid (Code No.: AG-EE 624 ZW) have been tested for their 2 blood sugar-lowering effect on female rats.
It was found, surprisingly, that the (S)-enantiomer (AG-EE 623 ZW) is the effective enantiomer and its effect lasts longer than 6 hours in the rat.
On the basis of these findings in the rat, it seems appropriate to use exclusively AG-EE 623 ZW in humans, thereby reducing the dose by 50%, compared with the dose of AG-EE 388 ZW. This and a relatively long period of activity have been found in humans. However, it was also found in the human studies that AG-EE 623 ZW has surprising pharmacokinetic properties which could not have been foreseen on the basis of the AG-EE 388 ZW data. AG-EE 623 ZW thus has surprising therapeutic advantages over the racemate AG-EE 388 ZW.
The surprising findings in humans are: The AG-EE 623 ZW levels fall more rapidly towards zero than the AG-EE 388 ZW levels, even when the dosage is absolutely the same, which could not be expected in view of the relatively long period of activity.
In relation to the lowering of blood sugar achieved, substantially lower plasma levels of AG-EE 623 ZW occur than might have been expected by halving the dosage of AG-EE 388 ZW.
The blood sugar lowering activity occurs more rapidly after the administration of AG-EE 623 ZW than after the administration of AG-EE 388 ZW.
The amazing difference between the two enantiomers is the fact that the effective enantiomer, AG-EE 623 ZW, in spite of having a relatively long period of activity, is surprisingly eliminated more rapidly than the ineffective enantiomer, AG-EE 624 ZW, as demonstrated by Figures 1 and 2.V After the administration of the 3 racemate, the ineffective enantiomer, AG-EE 624 ZW, is therefore present not only as an unnecessary additive in plasma concentrations which are just as high as those of the effective enantiomer, AG-EE 623 ZW, but is present in unexpectedly higher maximum and long-lasting levels.
The effect of this, e.g. on administration of a tablet containing 2 mg of AG-EE 388 ZW or one tablet containing 1 mg of AG-EE 623 ZW to 12 and 6 test subjects, respectively, is that the maximum concentrations are 84 25 and 28 18 ng/ml, respectively, and the concentrations after 4 hours are 19 8 and 0.7 1.0 ng/ml, respectively, after 5 hours 13 6 and 0.3 0.7 ng/ml, respectively, and after 6 hours 10 6 and 0.3 0.7 ng/ml, respectively.
The surprisingly quick onset of the lowering of blood sugar by AG-EE 623 ZW, compared with AG-EE 388 ZW, is particularly advantageous for diabetics, since the rapid onset results in optimum control of the disease.
Thus, compared with the administration of AG-EE 388 ZW, the surprising advantage of the administration of AG-EE 623 ZW is that unnecessarily high and long-lasting levels of the substance in the body are avoided, which is of major importance in long term therapy, such as that of diabetic mellitus.
Human studies have shown that the new (S)-enantiomer, namely (S)(+)-2-ethoxy-4-[N-[l-(2-piperidino-phenyl)-3methyl-l-butyl]aminocarbonylmethyl]-benzoic acid, as a vehicle of blood sugar-lowering activity, is far superior to AG-EE 388 ZW, because of its surprisingly rapid elimination from the blood, which was not foreseeable in view of its relatively long duration of activity, and these superior qualities go far beyond the "normal" advantage of an enantiomer over its racemate, namely the advantage of halving the dose.
4 The present invention therefore relates to the new (S)(+)-2-ethoxy-4-[N-[l-(2-piperidino-phenyl)-3-methyl- 1-butyl]-aminocarbonylmethyl]-benzoic acid or an 2-ethoxy-4-[N-[1-(2-piperidino-phenyl)-3-methyl-1butyl]-aminocarbonylmethyl]-benzoic acid, which is substantially optically pure, e.g. having an optical purity of at least ee 95%, preferably 98 to 100%, the physiologically acceptable salts thereof with inorganic or organic acids or bases, pharmaceutical compositions containing this compound or the physiologically acceptable salts thereof and processes for preparing them.
According to the invention, the new compound is obtained by the following methods: a) reaction of the (S)-amine of formula
CH
3 CH 3
CH
S NH 2 5 with a carboxylic acid of general formula
W
HOOCCH
2 OCHCH
(II)
wherein W represents a carboxy group or a carboxy group protected by a protecting group, or with the reactive derivatives thereof optionally prepared in the reaction mixture and, if necessary, subsequent cleaving of a protecting group.
Reactive derivatives of a compound of general formula II may be, for example., the esters thereof such as the methyl, ethyl or benzyl ester, the thioesters thereof such as the methylthio or ethylthioesters, the halides thereof such as acid chloride, the anhydrides or imidazolides thereof.
The reaction is conveniently carried out in a solvent such as methylene chloride, chloroform, carbon tetrachloride, ether, tetrahydrofuran, dioxane, benzene, toluene, acetonitrile or dimethylformamide, optionally in the presence of an acid-activating agent or a dehydrating agent, e.g. in the presence of ethylchloroformate, isobutylchloroformate, thionylchloride, phosphorus trichloride, phosphorus pentoxide, N,N'dicyclohexylcarbodiimide, N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide, N,N'-carbonyldiimidazole or N,N'-thionyldiimidazole or triphenylphosphine/carbon tetrachloride, or an agent which activates the amino group, e.g. phosphorus trichloride, and optionally in the presence of an inorganic base such as sodium carbonate or a tertiary organic base such as 6 triethylamine or pyridine which may simultaneously serve as solvent, at temperatures between -25 and 250*C, but preferably at temperatures between -10°C and the boiling temperature of the solvent used. The reaction may also be carried out without a solvent and moreover any water formed during the reaction may be removed by azeotropic distillation, e.g. by heating with toluene using a water separator, or by the addition of a drying agent such as magnesium sulphate or molecular sieve.
If necessary, the subsequent cleaving of a protecting group is preferably carried out by hydrolysis, conveniently either in the presence of an acid such as hydrochloric, sulphuric, phosphoric, trifluoroacetic or trichloroacetic acid or in the presence of a base such as sodium hydroxide or potassium hydroxide in a suitable solvent such as water, methanol, methanol/water, ethanol, ethanol/water, water/isopropanol or water/dioxane at temperatures between -10 and 120*C, e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture.
A tert.-butyl group used as protective group may also be cleaved thermally, optionally in an inert solvent such as methylene chloride, chloroform, benzene, toluene, tetrahydrofuran, dioxane or glacial acetic acid and preferably in the presence of a strong acid such as trifluoroacetic, hydrobromic, p-toluenesulphonic, sulphuric, phosphoric or polyphosphoric acid.
Moreover, a benzyl group used as protective group may also be cleaved hydrogenolytically in the presence of a hydrogenation catalyst such as palladium/charcoal in a suitable solvent such as methanol, ethanol, ethanol/water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide.
7 b) Cleaving an (S)-compound of general formula
CH
3 CH 3
CH
H 0C 2
H
(III)
wherein A represents a group which may be converted into a carboxy group by hydrolysis, thermolysis or hydrogenolysis.
Examples of hydrolysable groups include functional derivatives of the carboxy group such as the unsubstituted or substituted amides, esters, thioesters, orthoesters, iminoethers, amidines or anhydrides thereof, a nitrile group, a tetrazolyl group, an optionally substituted 1,3-oxazol-2-yl or 1,3-oxazolin- 2-yl group and examples of thermolytically cleavable groups include the esters with tertiary alcohols, e.g. a tert.butylester, and examples of hydrogenolytically cleavable groups include the aralkyl groups, e.g. a benzyl group.
The hydrolysis is conveniently carried out either in the presence of an acid such as hydrochloric, sulphuric, phosphoric, trifluoroacetic or trichloroacetic acid or in the presence of a base such as sodium hydroxide cr potassium hydroxide in a suitable solvent such as water, water/methanol, ethanol, water/ethanol, 1 *1 8 water/isopropanol or water/dioxane at temperatures between -10 and 120*C, e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture.
If A in a compound of general formula III represents a nitrile or aminocarbonyl group, these groups may be converted into the carboxy group by means of 100% phosphoric acid at temperatures between 100 and 180"C, preferably at temperatures between 120 and 160*C, or using a nitrite, e.g. sodium nitrite, in the presence of an acid such as sulphuric acid, whilst the latter may conveniently be used as solvent at the same time, at temperatures between 0 and If A in a compound of general formula III represents a tert.butyloxycarbonyl group, for example, the tert.butyl group may also be cleaved thermally, optionally in an inert solvent such as methylene chloride, chloroform, benzene, toluene, tetrahydrofuran, dioxane or glacial acetic acid and preferably in the presence of a strong acid such as trifluoroacetic acid, hydrobromic acid, ptoluenesulphonic acid, sulphuric acid, phosphoric acid or polyphosphoric acid, at temperatures between 0 and 100°C, preferably at temperatures between 20*C and the boiling temperature of the solvent used.
If A in a compound of general formula III represents a benzyloxycarbonyl group, for example, the benzyl group may also be cleaved hydrogenolytically in the presence of a hydrogenation catalyst such as palladium/charcoal in a suitable solvent such as methanol, ethanol, methanol/water, ethanol/water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide, preferably at temperatures between 0 and 50*C, e.g. at ambient temperature and under a hydrogen pressure of from 1 to bar.
9 c) Reaction of an (S)-compound of general formula
CH
3 CH 3
CH
SNH 0W c c C- ^N H
(IV)
wherein W' represents a carboxy group or an alkoxycarbonyl group having a total of 2 to 5 carbon atoms, wherein the alkyl moiety of the alkoxy group may be substituted by a phenyl group, with a compound of general formula Z CH 2
CH
3 (V) wherein Z represents a nucleophilically exchangeable group such as a halogen atom, a sulphonyloxy group or, together with the adjacent hydrogen atom, represents a diazo group, optionally followed by hydrolysis or hydrogenolysis.
The reaction is conveniently carried out with a corresponding haliae, sulphonic acid ester or sulphuric acid diester, e.g. with ethyl bromide, ethyl iodide, diethylsulphate, ethyl p-toluenesulphonate or ethylmethanesulphonate, or with diazoethane, optionally in the presence of a base such as sodium hydride, potassium carbonate, sodium hydroxide, potassium tert.butoxide or triethylamine, preferably in a suitable solvent such as acetone, diethylether, tetrahydrofuran, dioxane, 10 pyridine or dimethylformamide at temperatures between 0 and 100*C, preferably at temperatures between 20 and If W' in a compound of general formula IV represents a carboxy group, this can be converted into the corresponding ester compound.
If necessary, the subsequent hydrolysis is carried out either in the presence of an acid such as hydrochloric, sulphuric, phosphoric, trifluoroacetic or trichloroacetic acid or in the presence of a base such as sodium hydroxide or potassium hydroxide in a suitable solvent such as water, methanol, methanol/water, ethanol, ethanol/water, water/isopropanol or water/dioxane at temperatures between -10 and 120"C, e.g..at temperatures between ambient temperature and the boiling temperature of the reaction mixture, or the subsequent hydrogenolysis is carried out in the presence of a hydrogenation catalyst such as palladium/charcoal in a suitable solvent such as methanol, ethanol, ethanol/water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide under a hydrogen pressure of from 1 to 10 bar.
d) Enantioselective reduction of a compound of general formula y OCHCH 3
(VI)
11 wherein W' represents a carboxy group or an alkoxycarbonyl group having a total of 2 to 5 carbon atoms, wherein the alkyl moiety of the alkoxy group may be substituted by a phenyl .group, and Y .represents a group of the formula CH, CH 3
CH
3 H cl S CH C H 3 or N H-H and optional subsequent hydrolysis.
The reduction is preferably carried out with hydrogen in the presence of a suitable chiral hydrogenation catalyst in a suitable solvent such as methanol, ethanol, isopropanol, ethyl acetate, dioxane, tetrahydrofuran, methanol/tetrahydrofuran, methanol/methylene chloride, ethanol/methylene chloride of isopropanol/methylene chloride at temperatures between 0 and 100"C, but preferably at temperatures between 20 and 50"C, under a hydrogen pressure of between 1 and 1000 bar, preferably between 5 and 100 bar, and conveniently with the addition of 0.1 to preferably 0.3 to of titanium(IV)tetraisopropoxide, preferably with the exclusion of oxygen from the air. The reduction is preferably carried out with the (Z)-form of a compound of general formula VI.
Examples of chiral hydrogenation catalysts are the corresponding metal ligand c .aplexes such as Ru(OCO-
CH
3 2 [(S)-BINAP], Ru 2 C14[(S)-BINAP] 2 x N(C 2
H
5 3 4 or Rh[(-)-NORPHOS-COD]BF 4 During the catalytic hydrogenation, a benzyloxycarbonyl group may simultaneously be reduced and converted into 12 the carboxy group.
If recessary, the subsequent hydrolysis is carried out either in the presence of an acid such as hydrochloric, sulphuric, phosphoric, trifluoroacetic or trichloroacetic acid or in the presence of a base such as sodium hydroxide or potassium hydroxide in a suitable solvent such as water, methanol, methanol/water, ethanol, ethanol/water, water/isopropanol or water/dioxane at temperatures between -10 and 120"C, e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture.
e) Oxidation of an (S)-compound of general formula
CH
3
/CH
CH
[H
C H 2
CH
(VII)
wherein W" represents a group which may be converted into a carboxy group by oxidation.
An example of an oxidisable group of this kind might be a formyl group and the acetals thereof, a hydroxymethyl group and the ethers thereof, an unsubstituted or substituted acyl group such as acetyl, chloroacatyl, propionyl, malonic acid-(l)-yl group or a malonic ester- (1)-yl group.
The reaction is carried out with an oxidising agent in a suitable solvent such as water, glacial acetic acid, 0 1 13 methylene chloride, dioxane or glycoldimethylether at temperatures between 0 and 100"C, but ex '"iently at temperatures between 20 0 C and 50 0 C. Howe. r, the reaction is preferably carried out with silver oxide/sodium hydroxide solution, manganese dioxide/acetone or methylene chloride, hydrogen peroxide/sodium hydroxide solution, bromine or chlorine/sodium or potassium hydroxide solution, chromium trioxide/pyridine or pyridinium chlorochromate.
f) Separation of a mixture, consisting of any desired amount of the (S)-enantiomer of general formula
CH
3
CH
3
CHC
(VIII)
and any desired amount of the (R)-enantiomer of general formula
/"H
CH
CH
OCH
2
CH
3
H
(IX)
wherein W' represents a carboxy group or an alkoxycarbonyl group having a total of 2 to 5 carbon atoms, wherein the alkyl 14 moiety of the alkoxy group may be substituted by a phenyl group, preferably a 50/50 mixture, via the diastereomeric adducts, complexes or salts thereof, and followed if necessary by hydrolysis or hydrogenolysis.
The separation is preferably carried out using column or HPL chromatography by forming the diastereomeric adducts or complexes on a chiral phase.
If necessary, the subsequent hydrolysis is carried out either in the presence of an acid such as hydrochloric, sulphuric, phosphoric, trifluoroacetic or trichloroacetic acid or in the presence of a base such as sodium hydroxide or potassium hydroxide in a suitable solvent such as water, methanol, methanol/water, ethanol, ethanol/water, water/isopropanol or water/dioxane at temperatures between -10 and 120*C, e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture, or the subsequent hydrogenolysis is carried out in the presence of a hydrogenation catalyst such as palladium/charcoal in a suitable solvent such as methanol, ethanol, ethanol/water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide under a hydrogen pressure of from 1 to 10 bar.
The (S)-enantiomer thus obtained according to the invention, having an optical purity of, preferably, at least 90% can be converted by fractional crystallisation into an (S)-enantiomer having an optical purity of at least 95%, preferably 98 to 100%.
The same applies to the (S)-compounds according to the invention of formulae III, IV and VII, and more 15 particularly the esters thereof.
The (S)-enantiomer thus obtained according to the invention can be converted into the salts thereof, more particularly, for pharmaceutical use, into the physiologically acceptable salts thereof with inorganic or organic acids or bases. Examples of such acids include hydrochloric acid, hydrobromic acid, sulphuric acid, phosphoric acid, lactic acid, citric acid, tartaric acid, succinic acid, maleic acid or fumaric acid and examples of bases include sodium hydroxide, potassium hydroxide, calcium hydroxide, cyclohexylamine, ethanolamine, diethanolamine, triethanolamine, ethylenediamine or lysine.
The compounds of formulae I to IX used as starting materials are known from the literature in some cases or may be obtained by methods known per se.
The (S)-amine of formula I can be obtained from the corresponding racemic amine by racemate cleaving, e.g. by means of fractional crystallisation of the diastereomeric salts with suitable optically active acids, preferably with Nacetyl-L-glutamic acid, and if necessary recrystallisation and subsequent decomposition of the salts, by column or HPL-chromatography on chiral phases, optionally in the form of an acyl derivative, or by forming diastereomeric compounds, then separating and subsequently cleaving them.
Moreover, the (S)-amine of formula I may be prepared 16 by enantioselective reduction using hydrogen in the presence of a suitable chiral hydrogenation catalyst, starting from a corresponding N-acyl-ketimine or enamide, conveniently with the addition of 0.1 to titanium tetraisopropoxide, optionally with subsequent cleaving of the acyl group such as the formyl or acetyl group, by diastereoselective reduction of a corresponding ketimine or hydrazine chirally substituted at the nitrogen atom, using hydrogen in the presence of a suitable hydrogenation catalyst, expediently with the addition of 0.1 to 5% titanium tetraisopropoxide, and optionally followed by cleaving of the chiral auxiliary group, e.g. the (S)-l-phenethyl group, by catalytic hydrogenolysis, or by diastereoselective addition of a corresponding organometallic compound, preferably a Grignard or lithium compound, to a corresponding aldimine chirally substituted at the nitrogen atom, optionally with the addition of 0.1 to 10% titanium tetraisopropoxide, subsequent hydrolysis and optional separation of the resulting diastereomers and subsequent cleaving of the chiral auxiliary group, e.g. the (R)-l-phenethyl group by catalytic hydrogenolysis, and if necessary the (S)-amine may be obtained in a higher enantiomeric purity by salt formation with suitable optically active acids, preferably with Nacetyl-L-glutamic acid, and if necessary single or multiple recrystallisation and subsequent decomposition of the salt.
The compounds of general formulae III, IV and VII used as starting materials are obtained by reacting the amine I with a corresponding carboxylic acid or a
S
17 reactive derivative thereof and optionally subsequently splitting off any protecting group used.
The compound of general formula VI used as starting material is obtained by acylating the corresponding imino compound or the organometallic complexes thereof with the corresponding carboxylic acid or with the reactive derivatives thereof with optional subsequent cleaving of an ester group.
The new (S)-enantiomer is virtually non-toxic; for example, after a single administration of 1000 mg/kg p.o. (suspension in 1% methylcellulose) to 5 male and female rats, no animals died within the observation period of 14 days.
In view of its pharmacological and pharmacokinetic properties, the (S)-enantiomer prepared according to the invention (AG-EE 623 ZW) and the physiologically acceptable salts thereof are suitable for the treatment of diabetes mellitus. For this purpose, AG-EE 623 ZW or the physiologically acceptable salts thereof, optionally combined with other active substances, may be incorporated in the conventional galenic preparations such as plain or coated tablets, capsules, powders, suppositories, suspensions or injectable solutions. The single dose for adults is 0.1 to 20 mg, preferably 0.25 to 5 mg, especially 0.25, 0.5, 1.0, 1.5, 2.0, 2.5, or 5.0 mg, once, twice or three times a day.
The present invention further relates to the new amine of formula I which is a valuable intermediate product for preparing the new (S)-enantiomer, and the addition salts thereof with inorganic or organic acids.
The present invention also relates to the new compounds of general formulae III, IV and VII which are valuable 18 intermediate products for preparing the new enantiomer, and the addition salts thereof with inorganic or organic acids.
19 The Examples which follow are intended to illustrate the invention: Example A (S)-l-(2-Piperidino-phenyl)-3-methyl-l-butylamine A stirred solution of 122 g (0.495 mol) of racemic 1-(2piperidino-phenyl)-3-methyl-l-butylamine in 1000 ml of acetone is mixed with 93.7 g (0.495 mol) of N-acetyl-Lglutaminic acid. The mixture is refluxed over a vapour bath and methanol is added in batches (a total of about ml) until a clear solution is obtained. After this has been left to cool and stand overnight at ambient temperature, the crystals obtained are removed by suction filtering, washed twice with 200 ml of cold acetone at -15*C and then dried. The product obtained [98.9 g; melting point: 163-166*C; [a] 0 0.286° (c 1 in methanol)] is recrystallised from 1000 ml of acetone with the addition of 200 ml of methanol, thereby obtaining the (S)-1-(2-piperidino-phenyl)-3-methyl-lbutylamine as the addition salt of N-acetyl-L-glutaminic acid.
Yield: 65.1 g (60.4% of theory), Melting point: 168-171°C Calculated: C 63.42 H 8.56 N 9.65 Found: 63.64 8.86 9.60 0.357" (c 1 in methanol) The free amine is obtained as an oil by liberation, for example, with a sodium hydroxide or ammonia solution, extraction with toluene, ether, ethylacetate or methylene chloride, for example, and drying, filtering and evaporation of the extract in vacuo.
The (S)-configuration of the amine was demonstrated as follows: 20 Reaction of the amine with (S')-l-phenethylisocyanate in ether to obtain the corresponding urea derivative [melting point: 183-184"C; 2.25" (c 1 in methanol)], growing crystals from ethanol/water (8/1) and subsequent X-ray structural analysis showed the (S,S')-configuration for the urea derivative and consequently the (S)-configuration for the amine used.
Enantiomeric purity was determined as follows: 1. Acetylation of a sample of the amine with 1.3 equivalents of acetic anhydride in glacial acetic acid at 20"C overnight.
2. Investigation of the N-acetyl derivative (melting point: 128-132*C) by HPLC on a chiral phase HPLC column made by Baker, in which (S)-N-(3,5-dinitrobenzoyl)-2phenyl-glycine is covalently bonded to aminopropyl silica gel (particle size 5 gm, spherical, pore size A; column length: 250 mm with internal diameter 4.6 mm; eluant: n-hexane/isopropanol (100/5); flow rate: 2 ml/minute; temperature: 20*C; UV-detection at 254 nm.) Found: peak peak 2(S) 0.75%: 99.25%, ee (enantiomeric excess) 98.5% The (S)-amine may be converted into the dihydrochloride hydrate thereof using ethereal hydrogen chloride solution.
Melting point: 135-145*C (decomposition) Calc. (x H 2 C 56.99 H 8.97 N 8.31 Cl 21.02 Found: 56.85 8.93 8.38 21.25 [a]2 26.1° (c 1 in methanol) Example B N-Acetyl-N-[l-(2-piperidino-phenyl)-3-methyl-l-buten-lyl]-amine SAt ambient temperature, 4.7 ml (81.8 mMol) of glacial 21 acetic acid, 25.7 g (98.2 mMol) of triphenylphosphine, 34.2 ml (245 mMol) of triethylamine and 7.9 ml (81.8 mMol) of carbon tetrachloride are added to a solution of 20 g (81.8 mMol) of freshly prepared isobutyl-(2-piperidino-phenyl)-ketimine in 200 ml of acetonitrile and the resulting mixture is stirred for 18 hours at ambient temperature. It is then evaporated down in vacuo and distributed between ethyl acetate and water. The organic extract is dried and filtered and evaporated down in vacuo. The evaporation residue is purified by column chromatography on silica gel (toluene/ethyl acetate 10/1), eluting first the form and then the (Z)-form.
(E)-form: Yield: 6.1 g (26% of theory), Melting point: 135-137°C (ethylacetate/petroleum ether) Calculated: C 75.48 H 9.15 N 9.78 Found: 75.47 9.35 9.70 (Z)-form: Yield: 3.1 g (13% of theory), Melting point: 140-143"C (ethylacetate) Calculated: C 75.48 H 9.15 N 9.78 Found: 75.56 9.30 9.79 Example C N-Acetyl-N-[l-(2-piperidino-phenyl)-3-methyl-l-buten-lyl]-amine 17 ml (0.18 mol) of acetic anhydride are added dropwise, at an internal temperature of 0°C, to a stirred solution of 44 g (0.18 mol) of freshly prepared isobutyl-(2piperidino-phenyl)-ketimine in 440 ml of toluene. The mixture is stirred for a further 3 hours at 0°C and for hours at ambient temperature, then evaporated down in 22 vacuo, the evaporation residue is dissolved in ethyl acetate and extracted several times with aqueous sodium hydrogen carbonate solution. The organic phase is dried, filtered and evaporated down in vacuo. The evaporation residue is purified by column chromatography on silica gel (toluene/ethyl acetate eluting first the (E)-form and then the (Z)-form.
(E)-form: Yield: 3.0 g of theory), (Z)-form: Yield: 17.8 g (34.5% of theory), Melting point: 139-141'C (ethyl acetate) Calculated: C 75.48 H 9.15 N 9.78 Found: 75.68 8.99 9.86 Example D N-Acetyl-N-[(S)-l-(2-piperidino-phenyl)-3-methyl-lbutyl]-amine 0.57 g (1.99 mMol) of (Z)-N-acetyl-N-[l-(2-piperidinophenyl)-3-methyl-l-buten-l-yl]-amine, melting point 139-141°C, are dissolved in 10 ml of degassed solvent mixture (methanol/methylene chloride 5/1) under an Argon atmosphere and added to a solution of 16.8 mg (1 mol of the NOYORI-catalyst Ru(O-acetyl) 2
[(S)-BINAP]
(prepared from [Ru(COD)Cl12] with (S)-BINAP bis(diphenylphosphino)-1,1'-binaphthyl], triethylamine and sodium acetate), and 3.4 mg (0.5 mol of titanium tetraisopropoxide in 10 ml of degassed solvent mixture (methanol/methylene chloride The reaction mixture is drawn into an autoclave which is evacuated at 2 mbar. It is rinsed several times with hydrogen at 4 bar and the mixture is then hydrogenated at 30*C under 100 bar until the hydrogen uptake has ceased (170 hours). Then the reddish-brown solution is evaporated 4 23 down in vacuo, the evaporation residue is refluxed with ml of n-hexane and filtered hot to remove any insoluble matter. When the filtrate cools, crystallisation occurs.
Yield: 0.31 g (54% of theory), Melting point: 127-131C enantiomeric purity: ee 82% [HPLC method: see Example A].
14% of the racemic N-acetyl-amine of melting point 154-156*C can be obtained from the insoluble matter obtained when boiling with 30 ml of n-hexane, by further decoction with n-hexane, filtration and crystallisation from the hexane solution.
Example E (S)rl-(2-Piperidino-phenyl)-3-methyl-l-butylamine 1 g (3.47 mMol) of N-acetyl-N-[(S)-l-(2-piperidinophenyl)-3-methyl-l-butyl]-amine (melting point: 128-133"C; ee 99.4%] are refluxed in 10 ml of concentrated hydrochloric acid for 5.5 hours, then cooled and poured into a mixture of concentrated ammonia and ice. The mixture is extracted twice with ethyl acetate, the organic phase is washed with water, dried and filtered and then evaporated down in vacuo.
Yield: 0.84 g (98.8% of theory) oily amine.
By re-acetylation with 0.42 ml (1.3 equivalents) of acetic anhydride in 8.4 ml of glacial acetic acid overnight at ambient temperature, evaporation in vacuo, distribution of the evaporation residue between ethyl acetate and saturated aqueous sodium bicarbonate solution then drying, filtering and evaporation of the organic extract in vacuo, 0.83 g (84.7% of theory) of Nacetyl-N-[(S)-1-(2-piperidino-phenyl)-3-methyl-l-butyl]amine are obtained (melting point: 130-132°C; r I 24 ee 99.4%).
Example F Ethyl 2-ethoxy-4-[N-(1-(2-piperidino-phenyl)-3-methyl-lbuten-1-yl)-aminocarbonylmethyl]-benzoate Prepared from isobutyl-(2-piperidino-phenyl)-ketimine and 3-ethoxy-4-ethoxycarbonyl-phenylacetic acid analogously to Example B. Purification by column chromatography on silica gel (toluene/acetone 10/1), eluting first the (E)-form and then the (Z)-form.
(E)-form: Yield: 4% of theory, Melting point: 101-103°C Calculated: C 72.77 H 8.00 N 5.85 Found: 72.74 7.78 5.86 (Z)-form: Yield: 28.1% of theory, Melting point: 124-127°C (petroleum ether/toluene 5/1) Calculated: C 72.77 H 8.00 N 5.85 Found: 72.90 7.86 5.83 Example G N-[(S')-l-phenethyl]-N-[(S)-1-(2-piperidino-phenyl)-3methyl--butyl]-amine 17 g (49 mMol) of N-[(S')-l-phenethyl]-isobutyl-(2piperidino-phenyl)-ketimine, boiling point 150- 155*C/0.3 torr [prepared from isobutyl-(2-piperidinophenyl)-ketone and (S')-l-phenethyl-amine (made by Fluka, ee 99.6%) in toluene triethylamine by dropwise addition of a solution of titanium 25 tetrachloride in toluene] are dissolved in 170 ml of anhydrous ethanol. 1.7 g of titanium tetraisopropoxide and 8 g of Raney nickel are added and the mixture is hydrogenated at 50*C under 200 bar of hydrogen. After hours a further 8 g of Raney nickel are added and the mixture is hydrogenated for a further 52 hours under the same conditions. The catalyst is filtered off over a layer of Celite on a G3-mess and the filtrate is evaporated down in vacuo.
Yield: 13.1 g (76.6% of theory), Boiling point: 152"C/0.2 torr Calculated: C 82.23 H 9.78 N 7.99 Found: 82.00 10.03 7.74 [a]2o= 55.3" (c 1.1 in methanol) The diastereomeric purity is determined by HPLC on a Lichrosorb RP18 HPLC column made by E. Merck (Germany); column length: 250 mm with an internal diameter of 4 mm; particle size: 7 gm. Eluant: methanol/dioxane/0.1% aqueous sodium acetate solution, adjusted to pH 4.05 with acetic acid (135/60/5); temperature: 23*C; UVdetection at 254 nm.
Found: peak peak 98.4%: 1.4%, de (diastereomeric excess) 97.0% Example H (S)-1-(2-Piperidino-phenyl)-3-methyl-l-butylamine 12.5 g (36 mMol) of piperidino-phenyl)-3-methyl-l-butyl]-amine with a de of 97.0% are dissolved in 125 ml of water and 3.6 ml of conc. hydrochloric acid. 1.3 g of palladium/charcoal are added and the mixture is hydrogenated at 50*C under 5 bar of hydrogen. After the hydrogen uptake has ended (10 hours) the mixture is filtered over a layer of Celite to remove the catalyst.
26 The filtrate is made alkaline with conc. ammonia with the addition of ice and extracted with ethyl acetate.
The organic extract is dried and filtered and evaporated down in vacuo.
Yield: 6.4 g (72.1% of theory), Boiling point: 115-117"C/0.4 torr Enantiomeric purity: ee 93.5% rhPLC method (after previous acetylation): see Example A].
Example I N-[(R')-l-phenethyl]-N-[(S)-l-(2-piperidino-phenyl)-3methyl-l-butyl]-amine A solution of 2 g (6.84 mMol) of N-[(R')-l-phenethyl]- (2-piperidino-benzaldimine) [prepared from equimolar amounts of 2-piperidino-benzaldehyde and phenethylamine by standing overnight at ambient temperature and subsequent drying with sodium sulphate in ether solution] in 20 ml of anhydrous tetrahydrofuran is added dropwise to a solution of 27.4 mMol (4 equivalents) of isobutyl-magnesium bromide in 22 ml of anhydrous tetrahydrofuran, which is stirred in a bath at After 18 hours the bath temperature is increased to 80*C and a further 2 equivalents of isobutylmagnesium bromide in 11 ml of tetrahydrofuran are added.
After 12 hours stirring at 80°C 2 equivalents of isobutyl-magnesium bromide solution are added once again. After about 90 hours at 80°C the mixture is cooled, excess conc. hydrochloric acid is added and the resulting mixture is evaporated to dryness in a water jet vacuum. The evaporation residue is dissolved in water and made alkaline with conc. ammonia. It is extracted with ether, the organic extract is dried over sodium sulphate, filtered and evaporated in vacuo. The evaporation residue is purified by column chromatography on silica gel (toluene/acetone 95/5).
27 Yield: 0.20 g of theory), Melting point: The diastereomeric purity is determined by HPLC as in Example G.
Found: peak peak 4.4%:95.6%, de (diastereomeric excess) 91.2% In an analogous mixture with 2.0 g of the Schiff's base and a total of 6 equivalents of isobutyl-magnesium bromide in toluene/tetrahydrofuran and with the addition of 5% titanium(IV)-tetraisopropoxide and heating for 60 hours at 100 0 C in a glass tank, a yield of 5% was achieved with a de of 97.6% Example K (S)-1-(2-Piperidino-phenyl)-3-methyl-l-butylamine A solution of 0.15 g (0.428 mMol) of phenethyl]-N-[(S)-1-(2-piperidino-phenyl)-3-methyl-lbutyl]-amide (de 0.47 ml (0.47 mMol) of 1Nhydrochloric acid and 1.5 ml of water is hydrogenated in the presence of 20 mg of 10% palladium/charcoal for hours at 50°C under 3.4 bar of hydrogen. The mixture is filtered over kieselguhr, made alkaline with conc.
ammonia and extracted with ethyl acetate. The extract is dried, filtered and evaporated in vacuo.
Yield: 0.066 g (62.8% of theory), Melting point: Enantiomeric purity: ee 87.6% [HPLC method (after previous acetylation): see Example A].
28 Example 1 Ethyl (S)-2-ethoxy-4-[N-(l-(2-piperidino-phenyl)-3methyl-l-butyl)-aminocarbonylmethyl]-benzoate 0.48 g (1.91 mMol) of 3-ethoxy-4-ethoxycarbonylphenylacetic acid, 0.60 g (2.29 mMol) of triphenylphosphine, 0.80 ml (5.73 mMol) of triethylamine and 0.18 ml (1.91 mMol) of carbon tetrachloride are added successively to a solution of 0.47 g (1.91 mMol) of 3-methyl-l-(2-piperidino-phenyl)-1-butylamine (ee 98.5%) in 5 ml of anhydrous acetonitrile and the resulting mixture is stirred for 20 hours at ambient temperature. It is then evaporated down in vacuo and distributed between ethyl acetate and water. The organic extract is dried and filtered and evaporated down in vacuo. The evaporation residue is purified by column chromatography on silica gel (toluene/ethyl acetate 10/1).
Yield: 0.71 g (77.3% of theory), Melting point: 110-112°C Calculated: C 72.47 H 8.39 N 5.83 Found: 72.29 8.42 5.80 The enantiomeric purity is determined by HPLC on a chiral phase HPLC column made by Baker, in which is covalently bound to aminopropyl silica gel (particle size: 5 gm, spherical, A pore size; column length: 250 mm with an internal diameter of 4.6 mm; eluant: n-hexane/tetrahydrofuran/methylene chloride/ethanol (90/10/1/1); flow rate: 2 ml per minute; temperature: 20*C; UV detection at 242 nm).
Found: peak peak 2(S) 0.75%: 99.25%, ee 98.5% 29 Example 2 Ethyl (S)-2-ethoxy-4-[N-(l-(2-piperidino-phenyl)-3methyl-l-butyl)-aminocarbonylmethyl]-benzoate 2.77 g (11 mMol) of 3-ethoxy-4-ethoxycarbonylphenylacetic acid are added at ambient temperature to a solution of 2.71 g (11 mMol) of anhydrous (S)-3-methyll-(2-piperidino-phenyl)-l-butylamine (ee 98.5%) in ml of absolute toluene and the mixture is stirred until dissolved. Then 2.38 g (11.55 mMol) of N,N'dicyclohexyl-carbodiimide are added and the mixture is stirred at ambient temperature. After 24 hours a further 0.54 g (2.14 mMol) of 3-ethoxy-4-ethoxycarbonylphenylacetic acid and 0.48 g (2.33 mMol) of N,N'dicyclohexylcarbodiimide are added and the mixture is stirred overnight. It is then cooled to an internal temperature of +5°C and suction filtered to separate the precipitate, which is washed once with 5 ml of toluene.
The combined toluene filtrates are evaporated down in vacuo to a volume of about 10 ml. The resulting solution is heated over the steam bath and petroleum ether is added in batches thereto (total of 55 ml) until the turbidity remains. It is cooled in ice, whereupon crystallisation takes place. It is suction filtered and dried at 75 0 C/4 torr. The product obtained (4.57 g; melting point 111-112*C; ee 98.9%) is suspended in ml of petroleum ether. The mixture is heated over the steam bath and sufficient toluene is added in batches (8 ml in total) until a solution is obtained.
This is then cooled in ice and suction filtered to separate the crystals, which are dried at 75"C/4 torr.
Yield: 3.93 g (74.3% of theory), Melting point: 117-118°C Calculated: C 72.47 H 8.39 N 5.83 Found: 72.44 8.43 5.93 9.4" (c 1.01 in methanol) 30 Enantiomeric purity: ee 99.9% [HPLC method: see Example 1] Example 3 (S)-2-Ethoxy-4-[N-(l-(2-piperidino-phenyl)-3-methyl-lbutyl)-aminocarbonylmethyl]-benzoic acid A solution of 3.79 g (7.88 mMol) of ethyl (S)-2-ethoxy- 4-[N-(1-(2-piperidino-phenyl)-3-methyl-l-butyl)aminocarbonylmethyl]-benzoate (ee 99.9%) in 37 ml of ethanol is stirred in a bath at 60*C and 10 ml (10 mMol) of lN sodium hydroxide solution are added. After 4 hours stirring at 60"C, 10 ml (10 mMol) of 1Nhydrochloric acid are added in the warm and the mixture is left to cool to ambient temperature. After inoculation and standing overnight, the mixture is cooled for a further hour in ice, with stirring. The crystals are separated by suction filtering and washed twice with 5 ml of water. They are then dried at up to a final temperature of 100°C/4 torr in a vacuum drying cupboard over phosphorus pentoxide.
Yield: 3.13 g (87.7% of theory), Melting point: 130-131*C (high-melting form) Calculated: C 71.64 H 8.02 N 6.19 Found: 71.48 7.87 6.39 7.45" (c 1.06 in methanol) The enantiomeric purity is determined by HPLC on a chiral phase HPLC column made by ChromTech (Sweden) with an AGP(al-acid glycoprotein) phase; internal diameter: mm; length: 100 mm; particle diameter: 5 gm.
Temperature: 20"C; eluant: 0.1% aqueous KH 2
PO
4 solution 20% acetonitrile gradient increase within 4 minutes to 40% flow rate: 1 ml per minute; UV detection at 240 nm. Retention time (S)-enantiomer: 2.7 minutes; retention time (R)-enantiomer: 4.1 minutes.
31 Found: 99.85%: 0.15%, ee 99.7% When a sample is recrystallised from ethanol/water (2/1) the melting point does not change. When a sample is heated in petroleum ether/toluene the undissolved portion is filtered (melting point: 130-131"C) and the filtrate is rapidly cooled, the low melting form of the title compound is obtained, melting point 99-101*C.
Calculated: C 71.64 H 8.02 N 6.19 Found: 71.66 7.97 6.44 The low melting form and the high melting form differ in their infra-red KBr spectra but not in their infra-red solution spectra (methylene chloride).
If a sample of the low melting form is heated beyond its melting point a second melting point is observed at 127- 130 0
C.
If a sample of the low-melting form is recrystallised from ethanol/water the high melting form is obtained.
The high melting form and the low melting form were investigated by Differential Scanning Calorimetry (DSC) [Mettler apparatus, TA-300 system; measuring cell: DSC 20; made by Mettler, CH-8306 Greifensee, Switzerland] with the following results: 32 Compound of Heating rate 10°K/min. Heating rate 3"K/min.
Example 3 High melting Uniform melting peak Uniform melting peak form with melting tempera- with melting temperature of 133*C; of 132"C; melting enthalpy: melting enthalpy: 100 J/g 99.1 J/g Low melting 1st peak at 57*C 1st peak at 54°C form (very weak) (very weak; 2nd peak at 78"C endothermic) (weak) 2nd endothermic peak at 104*C.
3rd endothermic peak melting temperature at 107°C; 102 0
C,
melting enthalpy: melting enthalpy J/g 52 J/g 4th endothermic peak 3rd exothermic path at 132'C of the base line by melting enthalpy: crystallisation of J/g the substance melting at 104°C 4th endothermic peak at 131"C, melting temperature 130°C melting enthalpy 52 J/g Example 4 Ethyl (S)-2-ethoxy-4-[N-(l-(2-piperidino-phenyl)-3methyl-l-butyl)-aminocarbonylmethyl]-benzoate 0.79 g (1.65 mMol) of ethyl (Z)-2-ethoxy-4-[N-(l-(2piperidino-phenyl)-3-methyl--buten-l-yl)-aminocarbonylmethyl]-benzoate, melting point 124-127"C, are dissolved in 10.ml of degassed solvent mixture (methanol/methylene chloride 5/1) under an Argon atmosphere and added to a solution of 17 mg of the NOYORI-catalyst Ru(O- 33 acetyl)2[ (S)-BINAP] (prepared from [Ru(COD) C1 2 n with (S)-BINAP (S)-2,2'-bis-(diphenylphosphino)-l,l'binaphthyl], triethylamine and sodium acetate) and 3 mg of titanium tetraisopropoxide in 10 ml of degassed solvent mixture (methanol/methylene chloride 5/1).
The reaction mixture is drawn into an autoclave evacuated at 10-2 mbar. This is flushed five times with hydrogen at 5 bar and finally hydrogenated at 30*C and 100 bar until the hydrogen uptake has ceased (154 hours). The reddish-brown solution is evaporated down in vacuo, the evaporation residue is dissolved in 80 ml of ether, filtered off from the uncissolved brown flakes by means of activated charcoal and the resulting clear, bright yellow filtrate is evaporated down in vacuo. The evaporation residue (0.60 g) is refluxed in 60 ml of nhexane and filtered hot to separate it from the insoluble matter. The filtrate is left to stand overnight at ambient temperature. The crystals which are precipitated are filtered off.
Yield: 0.45 g (56.7% of theory), Melting point: 131-133 0 C (after sintering from 120°C) Enantiomeric purity: ee 39% [HPLC method: see Example 1].
Example Ethyl (S)-2-ethoxy-4-[N-(l-(2-piperidino-phenyl)-3methyl-l-butyl)-aminocarbonylmethyl]-benzoate 0.05 g (1.15 mMol) of 55% sodium hydride in oil are added to a solution of 0.68 g (1.15 mMol) of ethyl 2-hydroxy-4-[N-(1-(2-piperidino-phenyl)-3-methyl-lbutyl)-aminocarbonylmethyl]-benzoate [melting point: 125-126oC; 12.87" (c 1.01 in methanol)] in ml of anhydrous dimethylformamide and the mixture is stirred for 0.5 hours at ambient temperature. Then a solution of 0.12 ml (1.15 mMol) of ethyliodide in 2.5 ml 34 of anhydrous dimethylformamide is added dropwise thereto and the mixture is stirred for 5 hours at ambient temperature. It is evaporated down in vacuo, the residue is distributed between dilute sodium hydroxide solution and chloroform, the organic extract is dried, filtered and evaporated down in vacuo. The evaporation residue is purified by column chromatography on silica gel (toluene/ethyl acetate 10/1).
Yield: 0.48 g (67% of theory), Melting point: 110-112°C Calculated: C 72.47 H 8.39 N 5.83 Found: 72.61 8.54 5.97 Enantiomeric purity: ee 98.5% [HPLC method: see Example 1].
Example 6 Ethyl (S)-2-ethoxy-4-[N-(l-(2-piperidino-phenyl)-3methyl-l-butyl)-aminocarbonylmethyl]-benzoate Prepared from (S)-2-hydroxy-4-[N-(l-(2-piperidinophenyl)-3-methyl-l-butyl)-aminocarbonylmethyl]-benzoic acid analogously to Example 5 using 2 equivalents of sodium hydride and 2 equivalents of ethyl iodide.
Yield: 42% of theory, Melting point: 110-112°C Calculated: C 72.47 H 8.39 N 5.83 Found: 72.61 8.54 5.99 Enantiomeric purity: ee 98.3% [HPLC method: see Example 1].
35 Example 7 Ethyl (S)(+)-2-ethoxy-4-[N(-(-(2-piperidino-phenyl)-3methyl-1-butyl)-aminocarbonylmethyl]-benzoate and Ethyl (R)(-)-2-ethoxy-4-[N-(l-(2-piperidino-phenyl)-3methyl---butyl)-aminocarbonylmethyl]-benzoate 920 mg of ethyl (±)-2-ethoxy-4-[N-(l-(2-piperidinophenyl)-3-methyl-l-butyl)-aminocarbonylmethyl]-benzoate are separated, in single doses of 10 mg, on a preparative chiral phase HPLC column made by Baker, in which (S)-N-3,5-dinitrobenzoyl-leucine is covalently bonded to aminopropyl-silica gel (particle size: 40 pm; column length: 250 mm with an internal diameter of mm; eluant: n-hexane/tetrahydrofuran/ethanol/ methylene chloride (180/20/3/2); flow rate: 21.25 ml per minute; temperature: 27*C; UV-detection at 285 nm), in which first the (R)(-)-enantiomer (peak 1) and then the (S)(+)-enantiomer (peak 2) is eluted. After evaporation in vacuo, the following are obtained from the correspondingly cut and collected fractions: Peak 1 fraction 423 mg (crude), Peak 2 fraction 325 mg (crude).
In order to remove any impurities (including the stabiliser 2,6-di-tert.butyl-4-methyl-phenol contained in the tetrahydrofuran) the two fractions are each purified by column chromatography on silica gel (toluene/acetone 10/1).
(R)(-)-enantiomer: Yield: 234.5 mg (51% of theory), Melting point: 122-124*C (petroleum ether acetone) Calculated: C 72.47 H 8.39 N 5.83 Found: 72.40 8.18 5.71 [a] 0 8.3- (c 1 in methanol) (S)-enantiomer: Yield: 131.2 mg (28.5% of theory), 36 Melting point: 122-124"C (petroleum ether/acetone 8/1) Calculated: C 72.47 H 8.39 N 5.83 Found: 72.28 8.44 5.70 [a]2 0 8.3" (c 1 in methanol) A chiral cell OD column made by Daicel is also suitable for separating the enantiomers. The (R)-enantiomer is eluted after 6.8 minutes and the (S)-enantiomer after minutes on a column 250 mm long with an internal diameter of 4.6 mm (eluant: absolute ethanol/(n-hexane 0.2% diethylamine) 5/95; temperature: 40*C; UVdetection at 245 nm).
Example 8 (R)(-)-2-Ethoxy-4-[N-(l-(2-piperidino-phenyl)-3-methyll-butyl)-aminocarbonylmethyl]-benzoic acid x 0.4 HO 2 Prepared from 150 mg (0.312 mMol) of ethyl ethoxy-4-[N-(l-(2-piperidino-phenyl)-3-methyl-l-butyl)aminocarbonyl-methyl]-benzoate [melting point: 122- 124°C; 8.3" (c 1 in methanol)] by saponification with lN sodium hydroxide solution in ethanol analogously to Example 3.
Yield: 95.8 mg (66.7% of theory), Melting point: 103-105"C (toluene/petroleum ether) Calc. (x 0.4 H20): C 70.51 H 8.01 N 6.09 Found: 70.88 7.79 5.81 Molecular peak M Calculated: 452 Found: 452 6.5" (c 1 in methanol) Enantiomeric purity: ee 99.7% [HPLC method: see Example 3].
37 Example 9 (+)-2-Ethoxy-4-[N-(1-(2-piperidino-phenyl)-3-methyll-butyl)-aminocarbonylmethyl]-benzoic acid x 0.4 Prepared from 89 mg (0.198 mMol) of ethyl ethoxy-4-[N-(l-(2-piperidino-phenyl)-3-methyl-l-butyl)aminocarbonylmethyl]-benzoate [melting point: 122-124*C; 8.3° (c 1 in methanol)] by saponification with IN sodium hydroxide solution in ethanol analogously to Example 3.
Yield: 44.5 mg (48.8% of theory), Melting point: 102-103*C (toluene/petroleum ether) Calc.: (x 0.4 H 2 0) C 70.51 H 8.01 Found: 70.80 8.06 0= 6.7" (c 1 in methanol) Enantiomeric purity: ee 99.6% [HPLC method: see Example 3].
Example (S)-2-Ethoxy-4-[N-(1-(2-piperidino-phenyl)-3-methyl-lbutyl)-aminocarbonylmethyl]-benzoic acid 0.26 g (0.47 mMol) of benzyl (S)-2-ethoxy-4-[N-(l-(2pipieridino-phenyl)-3-methyl-l-butyl)-aminocarbonylmethyl]-benzoate (melting point: 91-92*C; [a] 20 c 1.05 in methanol) are hydrogenated in ml of ethanol using 0.12 g of palladium/charcoal at 50°C and 5 bar of hydrogen. After hours the catalyst is filtered off over kieselguhr and evaporated down in vacuo. The evaporation residue is crystallised from ethanol/water Yield: 0.15 g (70% of theory), Melting point: 130-131°C Calculated: C 71.64 H 8.02 N 6.19 38 Found: 71.76 8.12 6.05 Enantiomeric purity: ee 99.6% [HPLC method: see Example 3].
Example 11 (S)-2-Ethoxy-4-[N-(l-(2-piperidino-phenyl)-3-methyl-lbutyl)-aminocarbonylmethyl]-benzoic acid 102 mg (0.20 mMol) of tert.butyl (S)-2-ethoxy-4-[N-(l- (2-piperidino-phenyl)-3-methyl-l-butyl)-aminocarbonylmethyl]-benzoate (melting point: 122-123*C; [o]D c 1 in methanol) are refluxed in 5 ml of benzene together with a few crystals of ptoluenesulphonic acid hydrate, for half a day. The desired product is then obtained, according to thin layer chromatography, according to the R, value and mass spectrum.
Melting point: 129-131°C Molecular peak Calc.: 452 Found: 452 Example 12 (S)-2-Ethoxy-4-[N-(l-(2-piperidino-phenyl)-3-methyl-lbutyl)-aminocarbonylmethyl]-benzoic acid 200 mg (0.395 mMol) of tert.butyl (S)-2-ethoxy-4-[N-(l- (2-piperidino-phenyl)-3-methyl-l-butyl)-aminocarbonylmethyl]-benzoate (melting point: 122-123*C; c 1 in methanol) are stirred into 2 ml of methylene chloride together with 0.45 g (3.95 mMol) of trifluoroacetic acid overnight at ambient temperature. The mixture is evaporated down in vacuo and the evaporation residue is distributed between aqueous sodium hydrogen carbonate solution and ethyl 39 acetate. The organic extract is dried, filtered and evaporated down in vacuo. The evaporation residue is crystallised from ethanol/water Yield: 115 mg (64.7% of theory), Melting point: 126-128°C Calculated: C 71.64 H 8.02 N 6.19 Found: 71.39 7.91 6.06 0= 6.97" (c 0.975 in methanol) Enantiomeric purity: ee 99.8% [HPLC method: see Example 3].
Example 13 Tablets containing 0.25 mg AG-EE 623 ZW One tablet contains: 0.250 mg of active substance 0.125 mg of N-methylglucamine 0.038 mg of polyvinylpyrrolidone 0.075 mg of polyoxyethylenepolyoxypropylene polymer 0.150 mg of microcrystalline cellulose Preparation: The active substance and excipients are dissolved in water at 90°C or the microcrystalline cellulose is suspended and the dispersion is evaporated down in vacuo. The dry mass is screened to a mesh size of 1 mm.
The following ingredients are added to the granulated active substance, for each tablet: 24.862 mg of sodium carboxymethyl starch 24.000 mg of microcrystalline cellulose 0.500 mq of magnesium stearate 50.000 mg 40 Round, biplanar tablets weighing 50 mg and measuring mm in diameter are compressed from this mixture.
Example 14 Tablets containing 0.5 mg of AG-EE 623 ZW One tablet c.ntairs: 0.500 mj of active substance 0.250 mg of N-methylglucamine 0.075 mg of polyvinylpyrrolidone 0.150 mg of polyoxyethylenepolyoxypropylene polymer 0.300 mg of microcrystalline cellulose Preparation: The active *-tance and excipients are dissolved in water at 90 u a.,d the microcrystalline cellulose is suspended therein and the-dispersion is evaporated down in vacuo. The dry mass is screened to a mesh size of 1 mm.
The following ingredients are added to the active substance granul3s for each tablet: 24.225 mg of sodium _drboxymethyl starch 24,000 mg of microcrystalline cellulose 0.500 mq of magnesium stearate 50.000 mg Round, biplanar tablets weighing 50 mg and measuring mm in diameter are compressed from this mixture.
41 Example Tablets containing 1.0 mg of AG-EE 623 ZW One tablet contains: 1.00 mg of active substance 0.50 mg of N-methylglucamine 0.15 mg of polyvinylpyrrolidone 0.03 mg of polyoxyethylenepolyoxypropylene polymer 0.60 mg of microcrystalline cellulose Preparation: The active substance and excipients are dissolved in water at 90°C and the microcrystalline cellulose is suspended therein and the dispersion is evaporated down in vacuo. The dry mass is screened to a mesh size of 1 mm.
The following ingredients are added to the granulated active substance for each tablet: 23.22 mg of sodium carboxymethyl starch 24.00 mg of microcrystalline cellulose 0.50 mq of magnesium stearate 50.00 mg Round, biplanar tablets weighing 50 mg and measuring mm in diameter are compressed from this mixture.
Example 16 Tablets containing 1.5 mg of AG-EE 623 ZW One tablet contains: 1.500 mg of active substance 42 0.750 mg of N-methylglucamine 0.225 mg of polyvinylpyrrolidone 0.045 mg of polyoxyethylenepolyoxypropylene polymer 0.900 mg of microcrystalline cellulose Preparation: The active substance and excipients are dissolved in water at 90*C and the microcrystalline cellulose is suspended therein and the dispersion is evaporated down in vacuo. The dry mass is screened to a mesh size of 1 mm.
The following ingredients are added to the granulated active substance for each tablet: 23.080 mg of sodium carboxymethyl starch 23.000 mg of microcrystalline cellulose 0.500 mq of magnesium stearate 50.000 mg Round, biplanar tablets weighing 50 mg and measuring mm in diameter are compressed from this mixture.
Example 17 Tablets containing 2.0 mg of AG-EE 623 ZW One tablet contains: 2.00 mg of active substance 1.00 mg of L-lysine 1.00 mg of polyvinylpyrrolidone 1.00 mg of polyoxyethylenepolyoxypropylene polymer 4.00 mg of microcrystalline cellulose 43 Preparation: The ingredients are dissolved in water at 90*C and the microcrystalline cellulose is suspended therein and the dispersion is processed in a spray dryer. The following ingredients are then added for each tablet: 20.35 mg of microcrystalline cellulose 20.00 mg of sodium carboxymethyl starch 0.65 mq of magnesium stearate 50.00 mg Round, biconvex tablets weighing 50 mg and measuring mm in diameter are compressed from this mixture and are given a flavour-masking coating of hydroxypropylmethylcellulose.
Example 18 Tablets containing 2.5 mg of AG-EE 623 ZW One tablet contains: 2.50 mg of active substance 1.25 mg of L-lysine 1.25 mg of polyvinylpyrrolidone 1.25 mg of polyoxyethylenepolyoxypropylene polymer 4.10 mg of microcrystalline cellulose Preparation: The ingredients are dissolved in water at 90°C and the microcrystalline cellulose is suspended therein and the dispersion is processed in a spray dryer. Then the following ingredients are added for each tablet: 44 19.50 mg of microcrystalline cellulose 19.50 mg of sodium carboxymethyl starch 0.65 mg of magnesium stearate 50.00 mg Round, biconvex tablets weighing 50 mg and measuring mm in diameter are compressed from this mixture and given a flavour-masking coating of hydroxypropylmethyl cellulose.
Example 19 Tablets containing 3.0 mg of AG-EE 623 ZW One tablet contains: mg of active substance mg of L-lysine mg of polyvinylpyrrolidone mg of polyoxyethylenepolyoxypropylene polymer Preparation: The ingredients are dissolved in water at 90"C and the solution is processed in a spray dryer. Then, for each tablet, the following ingredients are added: 21.5 mg of microcrystalline cellulose 21.0 mq of sodium carboxymethyl starch 50.0 mg Round, biconvex tablets weighing 50 mg and measuring mm in diameter are compressed from this mixture and given a flavour-masking coating of hydroxypropylmethyl cellulose.
45 Example Tablets containing 5 mg of AG-EE 623 ZW One tablet contains: mg of active substance mg of L-lysine mg of polyvinylpyrrolidone mg of polyoxyethylenepolyoxypropylene polymer Preparation: The ingredients are dissolved in water at 90°C and the solution is processed in a spray dryer. Then, for each tablet, the following ingredients are added: 19.0 mg of microcrystalline cellulose 18.5 mq of sodium carboxymethyl starch 50.0 mg Round, biconvex tablets weighing 50 mg and measuring mm in diameter are compressed from this mixture and given a flavour-masking coating of hydroxypropylmethyl cellulose.
Claims (9)
1. (S)(+)-2-Ethoxy-4-[N-[l-(2-piperidino-phenyl)-3- methyl-l-butyl]aminocarbonylmethyl]-benzoic acid and the salts thereof with inorganic or organic acids or bases.
2. Compound according to claim 1 which is substantially optically pure.
3. Compound according to claim 2 having an optical purity of at least ee
4. Compound according to claim 2 having an optical purity of at least ee 98%. Physiologically acceptable salts of the compound according to claims 1 to 4 with organic or inorganic acids or bases.
6. Pharmaceutical compositions containing a compound according to claims 1 to 4 or a physiologically acceptable salt according to claim 5 together with one or more inert carriers and/or diluents.
7. Use of the compound according to claims 1 to 4 or the physiologically acceptable salts thereof according to claim 5 for treating diabetes mellitus.
8. Process for preparing pharmaceutical compositions according to claim 6, characterised in that a compound according to claims 1 to 4 or a physiologically acceptable salt according to claim 5 is incorporated in one or more inert carriers and/or diluents by a non- chemical method.
9. Process for preparing the new compound according to claims 1 to 5, characterised in that )1 J 47 a) the (S)-amine of formula CH CH, CH C c NH 2 N (I) is reacted with a carboxylic acid of general formula HOOCCH 2 OCH CH (II) wherein W represents a carboxy group or a carboxy group protected by a protecting group, or with a reactive derivative thereof, optionally prepared in the reaction mixture, and subsequently, if necessary, any protecting group used is cleaved or b) an (S)-compound of general formula 48 'NH/ '<^S0C H OCH) (III) wherein A represents a group which may be converted into a carboxy group by hydrolysis, thermolysis or hydrogenolysis is cleaved or c) an (S)-compound of general formula (IV) wherein W' represents a carboxy group or an alkoxycarbonyl having a total of 2 to 5 carbon atoms, wherein the moiety of the alkoxy group may be substituted by a phenyl group, is reacted with a compound of general formula group alkyl Z CH 2 CH 3 wherein Z represents a nucleophilically exchangeable group or, I 49 together with the adjacent hydrogen atom, represents a diazo group, and subsequently, if necessary, a compound thus obtained is hydrolysed or hydrogenolysed or d) a compound of general formula jW' Y "OCH 2 CH 3 (VI) wherein W' represents a carboxy group or an alkoxycarbonyl group having a total of 2 to 5 carbon atoms, wherein the alkyl moiety of the alkoxy group may be substituted by. a phenyl group, and Y represents a group of the formula CH, CH3 ¢H3 SCH, or 'HH/ N H is enantioselectively reduced and subsequently, if necessary, a compound thus obtained is hydrolysed or e) an (S)-compound of general formula CH 3 OHS CII (VII) 4; 9 50 wherein W" represents a group which can be converted into a carboxy group by oxidation is oxidised or f) a mixture consisting of any desired amount of the (S)-enantiomer of general formula CH 3 CH 3 C H 01 NH N H A'01 OC12CH3 (VIII) and any desired amount of the (R)-enantiomer of general formula CH 3 CH 3 0 CH (IX) wherein W' represents a carboxy group or an alkoxycarbonyl group having a total of 2 to 5 carbon atoms, wherein the alkyl moiety of the alkoxy group may be substituted by a phenyl group, is separated and a compound thus obtained is, if necessary converted by recrystallisation into a compound with a higher enantiomeric purity, crystallisation from ethanol/water producing the high melting form with a melting 4, 51 point of 130-131 C and crystallisation from petroleum ether/toluene producing the low-melting form with a melting point of 99-101*C, and a compound thus obtained is converted into the salts thereof, particularly into the physiologically acceptable salts thereof with organic or inorganic acids or bases. (S)-3-Methyl-l-(2-piperidino-phenyl)-1-butylamine and the acid addition salts thereof.
11. New compounds of general formulae C H CH3 t/ CH '1m 00 0 (III) 0 '0 H (IV) and 52 C\ CHCCHH H CH 2 CH 3 (VII) wherein A represents a group which can be converted into a carboxy group by hydrolysis, thermolysis or hydrogenolysis, W' represents a carboxy group or an alkoxycarbonyl group having a total of 2 to 5 carbon atoms, wherein the alkyl moiety of the alkoxy group may be substituted by a phenyl group, and W" represents a group which may be converted by oxidation into a carboxy group, and the addition salts thereof. -53 Abstract The present application relates to the new ethoxy-4-[N-[l-(2-piperidino-phenyl)-3-methyl-l-butyl]- aminocarbonylmethyl]-benzoic acid and the salts thereof, which have valuable pharmacological properties, namely an effect on the intermediate metabolism, but particularly the effect of lowering blood sugar. The invention also relates to the new intermediate products of formulae I, III, IV and VII and the addition salts thereof. The new compounds can be produced using methods which are known for analogous compounds.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002111851A CA2111851C (en) | 1991-06-21 | 1991-06-21 | (s)(+)-2-ethoxy-4-[n-{1-(2-piperidino-phenyl)-3-methyl-1-butyl]aminocarbonylmethyl]benzoic acid |
PCT/EP1991/001147 WO1993000337A1 (en) | 1991-06-21 | 1991-06-21 | (s)(+)-2-ethoxy-4-[n-[1-(2-piperidinophenyl)-3-methyl-1-butyl]aminocarbonylmethyl]benzoic acid |
SG1996002715A SG43036A1 (en) | 1991-06-21 | 1991-06-21 | (S) (+) -2-ethoxy-4-[N-[1-(2-piperidino-phenyl)-3-methyl- 1-buthyl]aminocarbony methyl] - benzoic a cid |
CN91104984A CN1048722C (en) | 1991-06-21 | 1991-07-22 | (S)(+)-2-Ethoxy-4-[N-[1-(2-piperidino-phenyl)-3-methyl-1-butyl]-aminocarbonylmethyl]-benzoic acid, pharmaceutical compositions containing compound and processes for preparation thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
AU8078191A AU8078191A (en) | 1993-01-25 |
AU660930B2 true AU660930B2 (en) | 1995-07-13 |
Family
ID=36781542
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU80781/91A Expired AU660930B2 (en) | 1991-06-21 | 1991-06-21 | (S)(+)-2-ethoxy-4-(N-(1-(2-piperidinophenyl)-3-methyl-1- butyl)aminocarbonylmethyl)benzoic acid |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0589874B1 (en) |
JP (1) | JP2921982B2 (en) |
CN (1) | CN1048722C (en) |
AU (1) | AU660930B2 (en) |
FI (1) | FI106027B (en) |
NO (2) | NO303687B1 (en) |
SG (1) | SG43036A1 (en) |
WO (1) | WO1993000337A1 (en) |
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AU7906898A (en) | 1997-06-13 | 1998-12-30 | Novo Nordisk A/S | Novel niddm regimen |
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PE20020617A1 (en) | 2000-08-22 | 2002-08-05 | Novartis Ag | COMPOSITION INCLUDING AN AT1 RECEPTOR ANTAGONIST AND AN INSULIN SECRETION POTENTIAL OR AN INSULIN SENSITIZER |
KR20040088519A (en) | 2002-02-22 | 2004-10-16 | 뉴 리버 파마슈티칼스, 인크. | Active Agent Delivery Systems and Methods for Protecting and Administering Active Agents |
US20040102477A1 (en) * | 2002-08-23 | 2004-05-27 | Dr. Reddy's Laboratories Limited | Polymorphic forms of (S)-Repaglinide and the processes for preparation thereof |
US7915421B2 (en) | 2003-05-14 | 2011-03-29 | Cilag Ltd. | Method for preparing phenyl acetic acid derivatives |
AU2003237595A1 (en) * | 2003-05-26 | 2004-12-13 | Biocon Limited | Process for the preparation of s(+)-2-ethoxy-4-(n-{1-(2-piperidinophelyl)-3-methyl-1- butyl} aminocarbonylmethyl)benzoic acid derivatives |
EP1664008A1 (en) * | 2003-08-28 | 2006-06-07 | Ranbaxy Laboratories Limited | Process for the preparation of amorphous form of repaglinide |
CN1305863C (en) * | 2004-12-27 | 2007-03-21 | 浙江大学 | Method for synthesizing (S)-isopropyl-(2-piperidine) phenyl-methylhistamine |
GT200600381A (en) | 2005-08-25 | 2007-03-28 | ORGANIC COMPOUNDS | |
FR2898894B1 (en) * | 2006-03-24 | 2008-06-06 | Genfit Sa | SUBSTITUTED N- (PHENETHYL) BENZAMIDE DERIVATIVE COMPOUNDS, PREPARATION AND USES |
US7763607B2 (en) * | 2006-04-27 | 2010-07-27 | Solvay Pharmaceuticals Gmbh | Pharmaceutical compositions comprising CBx cannabinoid receptor modulators and potassium channel modulators |
ES2618500T3 (en) * | 2006-10-20 | 2017-06-21 | Neurendo Pharma, Llc | Method to restore the incretin effect |
US8101769B2 (en) * | 2007-02-15 | 2012-01-24 | Actavis Group Ptc Ehf | Process for preparing ethyl (S)-2-ethoxy-4-[N-[1-(2-piperidinophenyl)-3-methyl-1-butyl]aminocarbonyl methyl]benzoate and use thereof for the preparation of Repaglinide |
US20100197732A1 (en) * | 2007-06-06 | 2010-08-05 | Actavis Group Ptc Ehf | Repaglinide Substantially Free of Dimer Impurity |
EP2019097A1 (en) | 2007-07-25 | 2009-01-28 | Aurobindo Pharma Limited | Process for preparing (alphaS)-alpha-(2-methylpropyl)-2-(1-piperidinyl)benzenemethanamine |
DE102008046995B4 (en) * | 2008-09-12 | 2010-08-26 | Stada Arzneimittel Ag | 2-ethoxy-benzoic acid |
EP2177221A1 (en) | 2008-10-20 | 2010-04-21 | Krka Tovarna Zdravil, D.D., Novo Mesto | Process for the preparation of substantially optically pure Repaglinide and precursors thereof |
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MX2011012627A (en) | 2009-05-28 | 2011-12-14 | Novartis Ag | Substituted aminobutyric derivatives as neprilysin inhibitors. |
JP5654608B2 (en) | 2009-11-17 | 2015-01-14 | ノバルティス アーゲー | Aryl-pyridine derivatives as aldosterone synthase inhibitors |
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CN102267959B (en) * | 2011-07-06 | 2013-05-01 | 海南锦瑞制药股份有限公司 | Repaglinide crystal, preparation method thereof, and solid oral preparation containing same |
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MX2016001020A (en) | 2013-07-25 | 2016-08-03 | Novartis Ag | Cyclic polypeptides for the treatment of heart failure. |
US10034871B2 (en) | 2014-11-07 | 2018-07-31 | Regents Of The University Of Minnesota | Salts and compositions useful for treating disease |
AU2016209968B2 (en) | 2015-01-23 | 2018-11-29 | Novartis Ag | Synthetic apelin fatty acid conjugates with improved half-life |
WO2018034627A1 (en) | 2016-08-18 | 2018-02-22 | İlko Ilaç Sanayi Ve Ticaret Anonim Şirketi | Pharmaceutical composition of antidiabetic tablet |
UY38072A (en) | 2018-02-07 | 2019-10-01 | Novartis Ag | COMPOSITIONS DERIVED FROM BUTANOIC ESTER SUBSTITUTED WITH BISPHENYL AS INHIBITORS OF NEP, COMPOSITIONS AND COMBINATIONS OF THE SAME |
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AU583631B2 (en) * | 1983-12-30 | 1989-05-04 | Dr. Karl Thomae Gmbh | Solid forms of 2-ethoxy-4-``N-(1-(2-piperidino-phenyl)-3- methyl-1-butyl)-aminocarbonylmethyl``-benzoic acid |
-
1991
- 1991-06-21 EP EP91911959A patent/EP0589874B1/en not_active Expired - Lifetime
- 1991-06-21 JP JP3511056A patent/JP2921982B2/en not_active Expired - Lifetime
- 1991-06-21 AU AU80781/91A patent/AU660930B2/en not_active Expired
- 1991-06-21 WO PCT/EP1991/001147 patent/WO1993000337A1/en active IP Right Grant
- 1991-06-21 SG SG1996002715A patent/SG43036A1/en unknown
- 1991-07-22 CN CN91104984A patent/CN1048722C/en not_active Expired - Lifetime
-
1993
- 1993-12-20 NO NO934726A patent/NO303687B1/en not_active IP Right Cessation
- 1993-12-20 FI FI935724A patent/FI106027B/en not_active IP Right Cessation
-
1999
- 1999-07-20 NO NO1999017C patent/NO1999017I1/en unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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AU583631B2 (en) * | 1983-12-30 | 1989-05-04 | Dr. Karl Thomae Gmbh | Solid forms of 2-ethoxy-4-``N-(1-(2-piperidino-phenyl)-3- methyl-1-butyl)-aminocarbonylmethyl``-benzoic acid |
Also Published As
Publication number | Publication date |
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WO1993000337A1 (en) | 1993-01-07 |
EP0589874A1 (en) | 1994-04-06 |
NO303687B1 (en) | 1998-08-17 |
AU8078191A (en) | 1993-01-25 |
NO934726D0 (en) | 1993-12-20 |
SG43036A1 (en) | 1997-10-17 |
FI935724A0 (en) | 1993-12-20 |
EP0589874B1 (en) | 1999-09-08 |
JPH06508816A (en) | 1994-10-06 |
JP2921982B2 (en) | 1999-07-19 |
FI106027B (en) | 2000-11-15 |
CN1048722C (en) | 2000-01-26 |
NO934726L (en) | 1993-12-20 |
CN1068820A (en) | 1993-02-10 |
FI935724A (en) | 1993-12-20 |
NO1999017I1 (en) | 1999-07-20 |
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