AU3537001A - Integrated microfluidic disc - Google Patents

Integrated microfluidic disc

Info

Publication number
AU3537001A
AU3537001A AU35370/01A AU3537001A AU3537001A AU 3537001 A AU3537001 A AU 3537001A AU 35370/01 A AU35370/01 A AU 35370/01A AU 3537001 A AU3537001 A AU 3537001A AU 3537001 A AU3537001 A AU 3537001A
Authority
AU
Australia
Prior art keywords
microfluidic disc
thermocycling reaction
chamber incorporating
integrated microfluidic
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU35370/01A
Inventor
Per Andersson
Nigel Eric Tooke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gyros Protein Technologies AB
Original Assignee
Gyros AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP1999/010347 external-priority patent/WO2000040750A1/en
Priority claimed from GBGB0011425.6A external-priority patent/GB0011425D0/en
Application filed by Gyros AB filed Critical Gyros AB
Publication of AU3537001A publication Critical patent/AU3537001A/en
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B01L2300/0806—Standardised forms, e.g. compact disc [CD] format
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/088—Passive control of flow resistance by specific surface properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Cereal-Derived Products (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Disclosed is a method for performing the steps of nucleic acid template purification, a thermocycling reaction and purification of the products of the thermocycling reaction characterised in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.
AU35370/01A 1999-12-23 2000-12-20 Integrated microfluidic disc Abandoned AU3537001A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
PCT/EP1999/010347 WO2000040750A1 (en) 1998-12-30 1999-12-23 Method for sequencing dna using a microfluidic device
WOEP99/10347 1999-12-23
GBGB0011425.6A GB0011425D0 (en) 1999-12-23 2000-05-12 Integrated microfluidic disc
GB0011425 2000-05-12
PCT/EP2000/013014 WO2001047638A2 (en) 1999-12-23 2000-12-20 Integrated microfluidic disc

Publications (1)

Publication Number Publication Date
AU3537001A true AU3537001A (en) 2001-07-09

Family

ID=26070393

Family Applications (1)

Application Number Title Priority Date Filing Date
AU35370/01A Abandoned AU3537001A (en) 1999-12-23 2000-12-20 Integrated microfluidic disc

Country Status (5)

Country Link
JP (1) JP4791670B2 (en)
AT (1) ATE329691T1 (en)
AU (1) AU3537001A (en)
DE (1) DE60028819T2 (en)
WO (1) WO2001047638A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7014815B1 (en) 1998-10-30 2006-03-21 Burstein Technologies, Inc. Trackable optical discs with concurrently readable nonoperational features
EP1284818B1 (en) * 2000-05-15 2006-11-22 Tecan Trading AG Bidirectional flow centrifugal microfluidic devices
US7026131B2 (en) 2000-11-17 2006-04-11 Nagaoka & Co., Ltd. Methods and apparatus for blood typing with optical bio-discs
US7087203B2 (en) 2000-11-17 2006-08-08 Nagaoka & Co., Ltd. Methods and apparatus for blood typing with optical bio-disc
WO2002046721A2 (en) 2000-12-08 2002-06-13 Burstein Technologies, Inc. Optical discs for measuring analytes
US7054258B2 (en) 2000-12-08 2006-05-30 Nagaoka & Co., Ltd. Optical disc assemblies for performing assays
US7091034B2 (en) 2000-12-15 2006-08-15 Burstein Technologies, Inc. Detection system for disk-based laboratory and improved optical bio-disc including same
JP4323806B2 (en) 2001-03-19 2009-09-02 ユィロス・パテント・アクチボラグ Characterization of reaction variables
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
US6726820B1 (en) 2001-09-19 2004-04-27 Applera Corporation Method of separating biomolecule-containing samples with a microdevice with integrated memory
US20080288178A1 (en) 2001-08-24 2008-11-20 Applera Corporation Sequencing system with memory
EP2281633A1 (en) 2001-08-28 2011-02-09 Gyros Patent Ab Retaining microfluidic microcavity and other microfluidic structures
US6919058B2 (en) 2001-08-28 2005-07-19 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
DE60237289D1 (en) 2001-09-17 2010-09-23 Gyros Patent Ab A CONTROLLED POWER IN A MICROFLUID DEVICE ENABLING FUNCTION UNIT
JP3952019B2 (en) * 2001-12-28 2007-08-01 株式会社日立ハイテクノロジーズ Extraction apparatus, chemical analysis apparatus, and chemical analysis method
US6532997B1 (en) 2001-12-28 2003-03-18 3M Innovative Properties Company Sample processing device with integral electrophoresis channels
AU2003224586A1 (en) * 2002-04-30 2003-11-17 Gyros Ab Integrated microfluidic device (ea)
US7005265B1 (en) 2002-06-20 2006-02-28 Wenhong Fan Nonenzymatic catalytic signal amplification for nucleic acid hybridization assays
JP2004354364A (en) 2002-12-02 2004-12-16 Nec Corp Fine particle manipulating unit, chip mounted with the same and detector, and method for separating, capturing and detecting protein
US7390464B2 (en) 2003-06-19 2008-06-24 Burstein Technologies, Inc. Fluidic circuits for sample preparation including bio-discs and methods relating thereto
US7238269B2 (en) * 2003-07-01 2007-07-03 3M Innovative Properties Company Sample processing device with unvented channel
US7776272B2 (en) 2003-10-03 2010-08-17 Gyros Patent Ab Liquid router
US7837947B2 (en) * 2003-12-12 2010-11-23 3M Innovative Properties Company Sample mixing on a microfluidic device
SE0400181D0 (en) * 2004-01-29 2004-01-29 Gyros Ab Segmented porous and preloaded microscale devices
US7187286B2 (en) 2004-03-19 2007-03-06 Applera Corporation Methods and systems for using RFID in biological field
TWI295730B (en) * 2004-11-25 2008-04-11 Ind Tech Res Inst Microfluidic chip for sample assay and method thereof
EP1874469A4 (en) 2005-04-14 2014-02-26 Gyros Patent Ab A microfluidic device with finger valves
US9110044B2 (en) 2005-05-25 2015-08-18 Boehringer Ingelheim Vetmedica Gmbh System for the integrated and automated analysis of DNA or protein and method for operating said type of system
US10816563B2 (en) 2005-05-25 2020-10-27 Boehringer Ingelheim Vetmedica Gmbh System for operating a system for the integrated and automated analysis of DNA or protein
US20090075801A1 (en) * 2007-09-19 2009-03-19 Dalibor Hodko Counter-centrifugal force device
WO2012033765A1 (en) 2010-09-07 2012-03-15 The Arizona Board Of Regents On Behalf Of The University Of Arizona Microdroplet-manipulation systems and methods for automated execution of molecular biological protocols
US9993819B2 (en) 2014-12-30 2018-06-12 Stmicroelectronics S.R.L. Apparatus for actuating and reading a centrifugal microfluidic disk for biological and biochemical analyses, and use of the apparatus
EP3523046B1 (en) 2016-10-07 2022-02-16 Boehringer Ingelheim Vetmedica GmbH Method and analysis system for testing a sample
EP3523035B1 (en) 2016-10-07 2020-07-29 Boehringer Ingelheim Vetmedica GmbH Analysis device and method for testing a sample
BR112020015495A2 (en) 2018-01-30 2020-12-08 Life Technologies Corporation INSTRUMENTS, DEVICES AND CONSUMABLES FOR USE IN A WORKFLOW OF AN INTELLIGENT MOLECULAR ANALYSIS SYSTEM

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
AU1115697A (en) * 1995-11-03 1997-05-22 Sarnoff Corporation Assay system and method for conducting assays
KR100306951B1 (en) * 1995-12-05 2001-11-15 테칸 보스턴, 인코포레이티드 Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
RO119751B1 (en) * 1997-02-28 2005-02-28 Burstein Laboratories, Inc. Optical disk, apparatus for conducting an optical control, method for detecting the presence or the absence of an analyte in a sample and method for controlling a biological, chemical or biochemical sample
US6013513A (en) * 1997-10-30 2000-01-11 Motorola, Inc. Molecular detection apparatus
GB9828785D0 (en) * 1998-12-30 1999-02-17 Amersham Pharm Biotech Ab Sequencing systems
AU5887000A (en) * 1999-06-22 2001-01-09 Tecan Trading Ag Devices and methods for the performance of miniaturized in vitro amplification assays

Also Published As

Publication number Publication date
WO2001047638A3 (en) 2002-05-30
DE60028819D1 (en) 2006-07-27
WO2001047638A2 (en) 2001-07-05
JP2003520582A (en) 2003-07-08
JP4791670B2 (en) 2011-10-12
ATE329691T1 (en) 2006-07-15
DE60028819T2 (en) 2007-01-11

Similar Documents

Publication Publication Date Title
AU3537001A (en) Integrated microfluidic disc
WO2005010209A3 (en) Method for the reverse transcription and/or amplification of nucleic acids
WO2001043871A3 (en) Microarray hybridization chamber
WO2004094635A3 (en) Compositions and methods for using a solid support to purify rna
WO2003020968A3 (en) Method for analyzing nucleic acid sequences and gene expression
EP1316599A4 (en) Magnetic particles and process for producing the same
WO2007076406A3 (en) A method of installing an epoxidation catalyst in a reactor, a process for the preparation of an olefin oxide or a chemical derivable from an olefin oxide, and a reactor suitable for such a process
EP0875584A3 (en) Nucleic acid assays
EP1464399A3 (en) Nucleic-acid amplifying apparatus and nucleic-acid amplifying method
WO2003060157A3 (en) Fluid manipulation in a microfabricated reaction chamber system
EP1300466A3 (en) A cascade nucleic acid amplification reaction
WO2003064691A3 (en) Methods and means for amplifying nucleic acid
ZA964057B (en) Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers.
AU3376897A (en) Microfluidic method for nucleic acid amplification
AU1491401A (en) Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus
DE69308162T2 (en) METHOD FOR AMPLIFICATING TARGET-SECTED SEGMENTS OF NUCLEIC ACID THROUGH THE INTERLOCKED POLYMERASE CHAIN REACTION
AU2965500A (en) Immobilized nucleic acid hybridization reagent and method
WO2008005248A3 (en) Real-time pcr in micro-channels
WO2007036526A3 (en) Reactor and method for treating a substance in a fluid reaction medium
WO2004048931A3 (en) Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
WO2003038120A3 (en) Method for analysis of genomic methylation models
WO2003004628A3 (en) Method for purifying an enzyme, a purified enzyme produced thereby, and use of this enzyme
WO2003048308A3 (en) Thermus brockianus nucleic acid polymerases
WO2005035087A3 (en) System and method for treating fluid using a multi-port valve assembly
WO2006119419A3 (en) Materials and kits for use in hot-start pcr, and methods of amplifying nucleic acids in a polymerase chain reaction

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase