AU2003210629A1 - Methods for the integrated synthesis and purification of oligonucleotides - Google Patents

Methods for the integrated synthesis and purification of oligonucleotides

Info

Publication number
AU2003210629A1
AU2003210629A1 AU2003210629A AU2003210629A AU2003210629A1 AU 2003210629 A1 AU2003210629 A1 AU 2003210629A1 AU 2003210629 A AU2003210629 A AU 2003210629A AU 2003210629 A AU2003210629 A AU 2003210629A AU 2003210629 A1 AU2003210629 A1 AU 2003210629A1
Authority
AU
Australia
Prior art keywords
oligonucleotides
purification
methods
integrated synthesis
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003210629A
Inventor
Michael Leuck
Wolfgang Pieken
Andreas Wolter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Proligo LLC
Original Assignee
Proligo LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proligo LLC filed Critical Proligo LLC
Publication of AU2003210629A1 publication Critical patent/AU2003210629A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AU2003210629A 2002-01-23 2003-01-22 Methods for the integrated synthesis and purification of oligonucleotides Abandoned AU2003210629A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35199102P 2002-01-23 2002-01-23
US60/351,991 2002-01-23
PCT/US2003/002008 WO2003062452A2 (en) 2002-01-23 2003-01-22 Methods for the integrated synthesis and purification of oligonucleotides

Publications (1)

Publication Number Publication Date
AU2003210629A1 true AU2003210629A1 (en) 2003-09-02

Family

ID=27613533

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003210629A Abandoned AU2003210629A1 (en) 2002-01-23 2003-01-22 Methods for the integrated synthesis and purification of oligonucleotides

Country Status (3)

Country Link
US (1) US7098326B2 (en)
AU (1) AU2003210629A1 (en)
WO (1) WO2003062452A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030153741A1 (en) * 2002-02-08 2003-08-14 Marek Kwiatkowski Methods for separating oligonucleotides
DE102005048503B4 (en) * 2005-10-07 2012-10-18 InViTek Gesellschaft für Biotechnik & Biodesign mbH Method for controlling the section-wise enzymatic nucleic acid amplification via incomplete complementary strands
US7850949B2 (en) * 2006-09-29 2010-12-14 Michigan Technological University Purification of synthetic oligomers
US7858772B2 (en) 2006-12-22 2010-12-28 Roche Molecular Systems, Inc. Compounds and methods for synthesis and purification of oligonucleotides
GB0814519D0 (en) 2008-08-08 2008-09-17 Imp Innovations Ltd Process
WO2012047639A2 (en) 2010-09-27 2012-04-12 Michigan Technological University Purification of synthetic oligonucleotides
JP2013151468A (en) 2011-11-30 2013-08-08 Agilent Technologies Inc Novel methods for synthesis and purification of oligomers

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US4112221A (en) 1974-07-17 1978-09-05 Asahi Kasei Kogyo Kabushiki Kaisha Process for preparing 8,2'-O-anhydropurine nucleosides
US4291024A (en) 1978-04-10 1981-09-22 Turcotte Joseph G Cytotoxic liponucleotide analogs
US4349552A (en) * 1978-10-30 1982-09-14 Fujisawa Pharmaceutical Company, Ltd. 5-Fluorouracil derivatives, and their pharmaceutical compositions
DE2931233A1 (en) 1979-08-01 1981-02-19 Mester Juliu Furo:coumarin derivs. prepn. - comprises condensing 7-hydroxy-coumarin derivs. with 4-chloro-1,3-dioxolanone, used for treating psoriasis
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4616071A (en) 1983-01-06 1986-10-07 Ford Motor Company Coating composition comprising bis-diene oligomers and bis-dieneophile oligomers
DE3329892A1 (en) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg METHOD FOR PRODUCING OLIGONUCLEOTIDES
US5118800A (en) 1983-12-20 1992-06-02 California Institute Of Technology Oligonucleotides possessing a primary amino group in the terminal nucleotide
US5118802A (en) 1983-12-20 1992-06-02 California Institute Of Technology DNA-reporter conjugates linked via the 2' or 5'-primary amino group of the 5'-terminal nucleoside
US5093232A (en) 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4816571A (en) 1987-06-04 1989-03-28 Applied Biosystems, Inc. Chemical capping by phosphitylation during oligonucleotide synthesis
US5420276A (en) 1987-11-03 1995-05-30 Abbott Laboratories Analogs of oxetanyl purines and pyrimidines
SE8802173D0 (en) 1988-06-10 1988-06-10 Astra Ab PYRIMIDINE DERIVATIVES
US5049656A (en) 1988-12-21 1991-09-17 Board Of Regents Of The University Of Nebraska Sequential peptide and oligonucleotide syntheses using immunoaffinity techniques
ATE190981T1 (en) 1989-10-24 2000-04-15 Isis Pharmaceuticals Inc 2'-MODIFIED NUCLEOTIDES
KR927003044A (en) 1990-01-11 1992-12-17 크리스토퍼 케이. 미라벨리 Composition and method for detecting and regulating RNA activity and gene expression
US5200514A (en) 1990-01-19 1993-04-06 University Of Georgia Research Foundation, Inc. Synthesis of 2'-deoxypyrimidine nucleosides
HUT61566A (en) 1990-03-13 1993-01-28 Acic Canada Inc Process for producing 2,2'-o-cyclonucleosides, nucleosides and their analogous compounds
GB9008696D0 (en) 1990-04-18 1990-06-13 Wellcome Found Anti-viral compounds
GB9021625D0 (en) 1990-10-04 1990-11-21 Ici Plc Synthesis of oligonucleotides
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
GB9207381D0 (en) 1992-04-03 1992-05-13 Ici Plc Synthesis of oligonucleotides
GB9225427D0 (en) 1992-12-04 1993-01-27 Ribonetics Gmbh Oligonucleotides with rna cleavage activity
AU6231794A (en) 1993-01-21 1994-08-15 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon, The Chemical functionalization of surfaces
AU6550394A (en) 1994-03-11 1995-09-25 Isis Pharmaceuticals, Inc. Novel pyrimidine nucleosides
EP0767657A4 (en) 1994-06-22 1999-01-20 Nexstar Pharmaceuticals Inc Novel method of preparation of known and novel 2'-modified nucleosides by intramolecular nucleophilic displacement
US5843650A (en) 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
US5874532A (en) 1997-01-08 1999-02-23 Nexstar Pharmaceuticals, Inc. Method for solution phase synthesis of oligonucleotides and peptides
KR19990064332A (en) * 1995-10-19 1999-07-26 래리 더블유. 스미스, 코크란 아담 Liquid phase synthesis method of oligonucleotide
WO1998020018A1 (en) * 1996-11-07 1998-05-14 Novartis Ag Process for the preparation of an oligomeric compound
EP2319854B1 (en) 1997-01-08 2016-11-30 Sigma-Aldrich Co. LLC Bioconjugation Of Macromolecules
EP0979233A4 (en) 1997-04-21 2001-03-21 Proligo L L C Method for solution phase synthesis of oligonucleotides
US6191286B1 (en) 1998-08-26 2001-02-20 Osi Specialties Inc. Imidosilane compositions
DE19935204A1 (en) 1999-07-27 2001-02-01 Bemauer Annette Labelling and masking of incomplete products in the production of polymeric compounds, especially oligonucleotides, comprises the addition of reactive functional compounds during the synthesis
US6171797B1 (en) 1999-10-20 2001-01-09 Agilent Technologies Inc. Methods of making polymeric arrays
EP1287404A4 (en) 2000-05-01 2003-10-15 Proligo Llc Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
JP2004503560A (en) 2000-06-13 2004-02-05 プロリゴ・エルエルシー Universal solid support for solid-phase oligo synthesis and methods of making and using it
EP1319012A1 (en) 2000-09-05 2003-06-18 University Technologies International Inc. Process for producing multiple oligonucleotides on a solid support

Also Published As

Publication number Publication date
WO2003062452A3 (en) 2003-12-04
US20030195351A1 (en) 2003-10-16
US7098326B2 (en) 2006-08-29
WO2003062452A2 (en) 2003-07-31

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase