AU2001286963A1 - Method of performing reverse transcription reaction using reverse transcriptase encoded by non-ltr retrotransposable element - Google Patents

Method of performing reverse transcription reaction using reverse transcriptase encoded by non-ltr retrotransposable element

Info

Publication number
AU2001286963A1
AU2001286963A1 AU2001286963A AU8696301A AU2001286963A1 AU 2001286963 A1 AU2001286963 A1 AU 2001286963A1 AU 2001286963 A AU2001286963 A AU 2001286963A AU 8696301 A AU8696301 A AU 8696301A AU 2001286963 A1 AU2001286963 A1 AU 2001286963A1
Authority
AU
Australia
Prior art keywords
transcription reaction
reverse transcriptase
reverse transcription
retrotransposable element
transcriptase encoded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001286963A
Inventor
Arkadiusz Bibillo
Thomas H. Eickbush
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Rochester
Original Assignee
University of Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Rochester filed Critical University of Rochester
Publication of AU2001286963A1 publication Critical patent/AU2001286963A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001286963A 2000-08-30 2001-08-30 Method of performing reverse transcription reaction using reverse transcriptase encoded by non-ltr retrotransposable element Abandoned AU2001286963A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22907500P 2000-08-30 2000-08-30
US60/229,075 2000-08-30
PCT/US2001/027125 WO2002018591A1 (en) 2000-08-30 2001-08-30 Method of performing reverse transcription reaction using reverse transcriptase encoded by non-ltr retrotransposable element

Publications (1)

Publication Number Publication Date
AU2001286963A1 true AU2001286963A1 (en) 2002-03-13

Family

ID=22859749

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001286963A Abandoned AU2001286963A1 (en) 2000-08-30 2001-08-30 Method of performing reverse transcription reaction using reverse transcriptase encoded by non-ltr retrotransposable element

Country Status (4)

Country Link
US (1) US7153672B1 (en)
EP (1) EP1320596A4 (en)
AU (1) AU2001286963A1 (en)
WO (1) WO2002018591A1 (en)

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CN101495656B (en) 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 DNA sequencing by nanopore using modified nucleotides
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
WO2012088339A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization using speed bumps
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
US9012183B2 (en) 2011-02-23 2015-04-21 Board Of Regents, The University Of Texas System Use of template switching for DNA synthesis
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
US9494554B2 (en) 2012-06-15 2016-11-15 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
EP3640349A3 (en) 2013-10-23 2020-07-29 Roche Sequencing Solutions, Inc. High speed molecular sensing with nanopores
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
CN106029904A (en) 2013-11-17 2016-10-12 宽腾矽公司 Integrated device with external light source for probing detecting and analyzing molecules
EP3194934B1 (en) 2014-08-08 2024-03-06 Quantum-Si Incorporated Integrated device for use with an external light source for probing, detecting, and analyzing molecules by luminescence lifetime measurements
KR102472061B1 (en) 2014-08-08 2022-11-29 퀀텀-에스아이 인코포레이티드 Optical system and assay chip for probing, detecting, and analyzing molecules
CN107112333B (en) 2014-08-08 2020-07-10 宽腾矽公司 Identification, imaging and sequencing method based on incident photon arrival time and storage medium
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
KR20180111999A (en) 2016-02-17 2018-10-11 테서렉트 헬스, 인코포레이티드 Sensors and devices for life-time imaging and detection applications
KR102489902B1 (en) * 2016-11-11 2023-01-19 바이오 래드 래버러토리스 인코오포레이티드 Methods of processing nucleic acid samples
EP4224530A1 (en) 2016-12-22 2023-08-09 Quantum-si Incorporated Integrated photodetector with direct binning pixel
CN112534049A (en) * 2018-05-16 2021-03-19 生物辐射实验室股份有限公司 Method for processing nucleic acid samples
AU2019288394A1 (en) 2018-06-22 2021-01-07 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time
JP2021530212A (en) * 2018-07-13 2021-11-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California Retrotransposon-based delivery medium and how to use it
CN112805373B (en) * 2018-08-08 2023-05-12 加利福尼亚大学董事会 Compositions and methods for ordered and continuous complementary DNA (cDNA) synthesis across non-contiguous templates
CN114040975A (en) * 2019-06-07 2022-02-11 生物辐射实验室股份有限公司 Multiple bead per droplet solution
US20230040216A1 (en) * 2019-11-19 2023-02-09 The Broad Institute, Inc. Retrotransposons and use thereof
EP4225941A1 (en) * 2020-10-09 2023-08-16 The Regents of the University of California Improvements to reactions with non-retroviral reverse transcriptase

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
US5350683A (en) 1990-06-05 1994-09-27 Immunex Corporation DNA encoding type II interleukin-1 receptors
US5817481A (en) * 1996-05-21 1998-10-06 Smithkline Beecham Corporation Polynucleotides and polypeptides associated with benign prostatic hypertrophy

Also Published As

Publication number Publication date
WO2002018591A9 (en) 2003-04-03
US7153672B1 (en) 2006-12-26
EP1320596A1 (en) 2003-06-25
US20060281079A1 (en) 2006-12-14
WO2002018591A1 (en) 2002-03-07
EP1320596A4 (en) 2005-01-12

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