AU2001265092A1 - Profiling of protease specificity using combinatorial fluorogenic substrate libraries - Google Patents

Profiling of protease specificity using combinatorial fluorogenic substrate libraries

Info

Publication number
AU2001265092A1
AU2001265092A1 AU2001265092A AU6509201A AU2001265092A1 AU 2001265092 A1 AU2001265092 A1 AU 2001265092A1 AU 2001265092 A AU2001265092 A AU 2001265092A AU 6509201 A AU6509201 A AU 6509201A AU 2001265092 A1 AU2001265092 A1 AU 2001265092A1
Authority
AU
Australia
Prior art keywords
profiling
fluorogenic substrate
protease specificity
substrate libraries
combinatorial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001265092A
Inventor
Bradley J. Backes
Charles S Craik
Jonathan A Ellman
Jennifer L. Harris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California filed Critical University of California
Publication of AU2001265092A1 publication Critical patent/AU2001265092A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/101—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms, e.g. Val, Ile, Leu
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1024—Tetrapeptides with the first amino acid being heterocyclic
AU2001265092A 2000-06-02 2001-05-25 Profiling of protease specificity using combinatorial fluorogenic substrate libraries Abandoned AU2001265092A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US20927400P 2000-06-02 2000-06-02
US60209274 2000-06-02
PCT/US2001/017265 WO2001094332A1 (en) 2000-06-02 2001-05-25 Profiling of protease specificity using combinatorial fluorogenic substrate libraries
US09/866,132 US6680178B2 (en) 2000-06-02 2001-05-25 Profiling of protease specificity using combinatorial fluorogenic substrate libraries
US09866132 2001-05-25

Publications (1)

Publication Number Publication Date
AU2001265092A1 true AU2001265092A1 (en) 2001-12-17

Family

ID=26903999

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001265092A Abandoned AU2001265092A1 (en) 2000-06-02 2001-05-25 Profiling of protease specificity using combinatorial fluorogenic substrate libraries

Country Status (3)

Country Link
US (2) US6680178B2 (en)
AU (1) AU2001265092A1 (en)
WO (1) WO2001094332A1 (en)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6680178B2 (en) * 2000-06-02 2004-01-20 The Regents Of The University Of California Profiling of protease specificity using combinatorial fluorogenic substrate libraries
ATE359513T1 (en) * 2000-06-13 2007-05-15 Glaxo Group Ltd HIGH-THROUGHPUT SCREENING OF COMPOUNDS FOR BIOLOGICAL EFFECTIVENESS
US6797461B2 (en) * 2000-10-27 2004-09-28 Promega Corporation Tryptase substrates and assay for tryptase activity using same
US7687437B2 (en) * 2001-07-13 2010-03-30 Nanosphere, Inc. Method for immobilizing molecules onto surfaces
US7297553B2 (en) * 2002-05-28 2007-11-20 Nanosphere, Inc. Method for attachment of silylated molecules to glass surfaces
CA2652991A1 (en) * 2001-07-16 2003-11-13 Caprotec Bioanalytics Gmbh Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
DE60325847D1 (en) * 2002-03-11 2009-03-05 Caprotec Bioanalytics Gmbh COMPOUNDS AND PROCEDURES FOR THE ANALYSIS OF THE PROTEOM
EP1361284A1 (en) * 2002-05-10 2003-11-12 Direvo Biotech AG Process for generating sequence-specific proteases by directed evolution and use thereof
US20040072276A1 (en) * 2002-05-10 2004-04-15 Direvo BioTech AG. Process for generating sequence-specific proteases by directed evolution and use thereof
US7939304B2 (en) * 2002-10-02 2011-05-10 Catalyst Biosciences, Inc. Mutant MT-SP1 proteases with altered substrate specificity or activity
CN101124484A (en) * 2002-10-02 2008-02-13 催化剂生物科学有限公司 Methods of generating and screening for proteases with altered specificity
US20060024289A1 (en) * 2002-10-02 2006-02-02 Ruggles Sandra W Cleavage of VEGF and VEGF receptor by wild-type and mutant proteases
AU2004206856B9 (en) * 2003-01-16 2006-11-02 Caprotec Bioanalytics Gmbh Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
WO2005023981A2 (en) * 2003-09-09 2005-03-17 Zephyr Proteomix Ltd. Libraries of chimeric cellulose binding proteins and methods of use thereof
GB0400974D0 (en) * 2004-01-16 2004-02-18 Solexa Ltd Multiple inexact matching
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
CA2562729C (en) * 2004-04-12 2013-11-12 Sandra Waugh Ruggles Cleavage of vegf and vegf receptor by wildtype and mutant mt-sp1
EP1735438A2 (en) * 2004-04-12 2006-12-27 Catalyst Biosciences Cleavage of vegf and vegf receptor by wild-type and mutant proteases
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US7329506B2 (en) * 2004-12-03 2008-02-12 Rutgers, The State University Apparatuses and methods for determining protease activity
KR100665964B1 (en) 2005-01-31 2007-01-09 건국대학교 산학협력단 Anticancer composition containing Abc-ADC-AMC
US8658372B2 (en) * 2005-06-10 2014-02-25 The United States Of America, As Represented By The Secretary Of The Navy Affinity-based detection of biological targets
AU2006272496B2 (en) 2005-07-27 2013-03-07 University Of Southern California Coumarin-based amino acids for use in enzyme activity and substrate specificity assays
KR101393946B1 (en) * 2005-10-21 2014-05-12 카탈리스트 바이오사이언시즈, 인코포레이티드 Modified proteases that inhibit complement activation
JP2009536313A (en) 2006-01-11 2009-10-08 レインダンス テクノロジーズ， インコーポレイテッド Microfluidic devices and methods for use in nanoreactor formation and control
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (en) * 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
KR101778174B1 (en) * 2006-07-05 2017-09-13 카탈리스트 바이오사이언시즈, 인코포레이티드 Protease screening methods and proteases identified thereby
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
US8772046B2 (en) * 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2009117524A2 (en) * 2008-03-18 2009-09-24 The Regents Of The University Of California Sparse matrix system and method for identification of specific ligands or targets
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US9782565B2 (en) 2008-10-01 2017-10-10 Covidien Lp Endoscopic ultrasound-guided biliary access system
US8968210B2 (en) * 2008-10-01 2015-03-03 Covidien LLP Device for needle biopsy with integrated needle protection
US11298113B2 (en) 2008-10-01 2022-04-12 Covidien Lp Device for needle biopsy with integrated needle protection
US9332973B2 (en) 2008-10-01 2016-05-10 Covidien Lp Needle biopsy device with exchangeable needle and integrated needle protection
US9186128B2 (en) 2008-10-01 2015-11-17 Covidien Lp Needle biopsy device
EP3415235A1 (en) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation of microfluidic droplets
EP2443136A4 (en) * 2009-06-18 2013-01-09 Strongbone Ab Novel peptide derivatives for treatment, prevention or alleviation of a condition associated with bone loss or low bone density or to inhibit osteoclast differentiation and stimulation
RU2012101779A (en) 2009-06-19 2013-07-27 МЕДИММЬЮН, ЭлЭлСи PROSTHETIC OPTIONS
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
JP5934657B2 (en) 2010-02-12 2016-06-15 レインダンス テクノロジーズ， インコーポレイテッド Digital specimen analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
WO2018222773A2 (en) * 2017-05-30 2018-12-06 Chan Eugene Y Fluorogenic peptide substrates for in solution and solid phase factor xa measurements
US11613744B2 (en) 2018-12-28 2023-03-28 Vertex Pharmaceuticals Incorporated Modified urokinase-type plasminogen activator polypeptides and methods of use
CN113661239A (en) 2018-12-28 2021-11-16 催化剂生物科学公司 Modified urokinase-type plasminogen activator polypeptides and methods of use
CN115109066B (en) * 2022-06-30 2023-04-07 武汉工程大学 Fluorescent probe for detecting elastase through large Stokes displacement and preparation method and application thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE7801373L (en) 1978-02-07 1979-08-08 Kabi Ab EASY SPLABLE SUBSTRATE FOR QUANTIFIATION OF PROTEASES
US4568636A (en) 1981-03-25 1986-02-04 Pentapharm Ag Tripeptide derivatives
US4448715A (en) 1981-11-02 1984-05-15 University Of Miami Tagged pyroglu-L-Phe-L-Arg derivatives, substrates and assays for kallikrein
US5079144A (en) 1982-04-14 1992-01-07 Radiometer Corporate Development Ltd. Microorganism testing with a hydrolyzable fluorogenic substrate
US4640893A (en) 1983-10-28 1987-02-03 University Of Illinois Novel rhodamine derivatives as fluorogenic substrates for proteinases
US4557862A (en) 1983-10-28 1985-12-10 University Patents, Inc. Rhodamine derivatives as fluorogenic substrates for proteinases
US5089634A (en) * 1984-08-20 1992-02-18 Georgia Tech Research Corporation Isocoumarins with cationic substituents
US4897444A (en) * 1985-05-31 1990-01-30 The Research Foundation Of The State University Of New York Immobilized fluorogenic substrates for enzymes; and processes for their preparation
FR2677023B1 (en) 1991-05-30 1994-02-25 Eurobio Laboratoires WATER-SOLUBLE COUMARIN DERIVATIVES, THEIR PREPARATION AND THEIR USE AS ENZYME SUBSTRATE.
US5872207A (en) * 1992-09-04 1999-02-16 Sanofi Pharmaceuticals, Inc. N-terminal-marked peptides immobilized on glass beads and method of preparation and method of use thereof
JP3595907B2 (en) * 1996-06-21 2004-12-02 デイド、ベーリング、インコーポレイテッド Compositions and methods for making ion-selective electrodes
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
GB2324529A (en) * 1997-02-21 1998-10-28 Merck & Co Inc A combinatorial library based on a tetrapeptide substituted with aminomethylcoumarin for characterizing proteases
GB2324509A (en) 1997-04-24 1998-10-28 Rover Group Vehicle airbag housing assembly
US6680178B2 (en) * 2000-06-02 2004-01-20 The Regents Of The University Of California Profiling of protease specificity using combinatorial fluorogenic substrate libraries

Also Published As

Publication number Publication date
US7629437B2 (en) 2009-12-08
WO2001094332A1 (en) 2001-12-13
US20020022243A1 (en) 2002-02-21
US6680178B2 (en) 2004-01-20
US20040175777A1 (en) 2004-09-09

Similar Documents

Publication Publication Date Title
AU2001265092A1 (en) Profiling of protease specificity using combinatorial fluorogenic substrate libraries
NO20003332D0 (en) Midisin precursor of aspartyl protease inhibitors
EP1469873A4 (en) Peptidomimetic inhibitors of post-proline cleaving enzymes
PL362646A1 (en) Novel mixtures of microbial enzymes
ZA200402501B (en) Inhibitors of post-proline cleaving proteases.
HK1070008A1 (en) Combination of cytochome p 450 dependent protease inhibitors
NO20016034D0 (en) Inhibitors of aspartyl protease
AU2002339348A1 (en) Inhibition of STAT-1
AU2108601A (en) Salicylamides as serine protease inhibitors
AU2001265182A1 (en) Inhibitors of matrix metalloproteinases
HK1061700A1 (en) An assay for detecting inhibitors of the enzyme myeloperokidase
AU2002323061A1 (en) Alteration of acyltransferase domain substrate specificity
AU2252101A (en) New synthesis of SPLA2 inhibitors
AU2002236120A1 (en) Fast dissolving tablets of cyclooxygenase-2 enzyme inhibitors
AUPR079700A0 (en) Combinatorial libraries of monosaccharides
AU2002342682A1 (en) Protease inhibitors
AU2003249320A1 (en) Novel inhibitors of ubiquitin isopeptidases
AU2002348465A1 (en) Alpha-hydroxyarylbutanamine inhibitors of aspartyl protease
AU2001252922A1 (en) Azide cyclization-based synthesis and intermediates for spla2 inhibitors
AU2002363791A1 (en) Protease enzyme inhibitors
AU2002305868A1 (en) Inhibitors of reggamma
AU2002364185A1 (en) Affinity labeling of serine proteases for simultaneous detection of multiple serine protease activity levels
AU2002331752A1 (en) Combinatorial protease substrate libraries
GB0109278D0 (en) Enzyme inhibitors
AU6532300A (en) Synthesis of protease substrates