AU2001262284A1 - Method for detecting polynucleotides using fluorescence correlation spectroscopy - Google Patents

Method for detecting polynucleotides using fluorescence correlation spectroscopy

Info

Publication number
AU2001262284A1
AU2001262284A1 AU2001262284A AU6228401A AU2001262284A1 AU 2001262284 A1 AU2001262284 A1 AU 2001262284A1 AU 2001262284 A AU2001262284 A AU 2001262284A AU 6228401 A AU6228401 A AU 6228401A AU 2001262284 A1 AU2001262284 A1 AU 2001262284A1
Authority
AU
Australia
Prior art keywords
polynucleotides
correlation spectroscopy
fluorescence correlation
detecting polynucleotides
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001262284A
Inventor
Zeno Foldes-Papp
Rudolf Rigler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gnothis Holding SA
Original Assignee
Gnothis Holding SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10065632A external-priority patent/DE10065632A1/en
Application filed by Gnothis Holding SA filed Critical Gnothis Holding SA
Publication of AU2001262284A1 publication Critical patent/AU2001262284A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention relates to a method for detecting polynucleotides on a substrate, whereby the substrate contains a multitude of separate detection regions each accommodating different polynucleotides. The polynucleotides in the detection regions exist, contrary to known DNA chip test formats, in a form that is not bound to the substrate. The invention also relates to a device for implementing said method.
AU2001262284A 2000-05-12 2001-05-11 Method for detecting polynucleotides using fluorescence correlation spectroscopy Abandoned AU2001262284A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE10023421.6 2000-05-12
DE10023421 2000-05-12
DE10065632A DE10065632A1 (en) 2000-05-12 2000-12-29 Detecting polynucleotides by hybridizing an array of sequences on a carrier with detection probes, but not immobilized
DE10065632.3 2000-12-29
PCT/EP2001/005410 WO2001085991A2 (en) 2000-05-12 2001-05-11 Method for detecting polynucleotides using fluorescence correlation spectroscopy

Publications (1)

Publication Number Publication Date
AU2001262284A1 true AU2001262284A1 (en) 2001-11-20

Family

ID=26005660

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001262284A Abandoned AU2001262284A1 (en) 2000-05-12 2001-05-11 Method for detecting polynucleotides using fluorescence correlation spectroscopy

Country Status (5)

Country Link
EP (1) EP1280939B1 (en)
AT (1) ATE394507T1 (en)
AU (1) AU2001262284A1 (en)
DE (1) DE50113940D1 (en)
WO (1) WO2001085991A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
DE10225841A1 (en) * 2002-06-03 2003-12-11 Proteosys Ag Differential display of labeled molecules
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1716254B1 (en) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Methods for analyzing polynucleotide sequences
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2017027740A1 (en) 2015-08-11 2017-02-16 Microvention, Inc. System and method for implant delivery

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2116578T3 (en) * 1993-01-18 1998-07-16 Evotec Biosystems Gmbh PROCEDURE AND DEVICE TO EVALUATE THE APTITUDE OF BIOPOLYMERS.
AU7398996A (en) * 1995-10-11 1997-04-30 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6023540A (en) * 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
WO2001000875A2 (en) * 1999-06-25 2001-01-04 Motorola, Inc. Novel methods and products for arrayed microsphere analysis
WO2001014589A2 (en) * 1999-08-20 2001-03-01 Luminex Corporation Liquid array technology

Also Published As

Publication number Publication date
EP1280939A2 (en) 2003-02-05
DE50113940D1 (en) 2008-06-19
WO2001085991A2 (en) 2001-11-15
WO2001085991A3 (en) 2002-12-12
ATE394507T1 (en) 2008-05-15
EP1280939B1 (en) 2008-05-07

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