AU2001241939A1 - Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation - Google Patents

Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation

Info

Publication number
AU2001241939A1
AU2001241939A1 AU2001241939A AU4193901A AU2001241939A1 AU 2001241939 A1 AU2001241939 A1 AU 2001241939A1 AU 2001241939 A AU2001241939 A AU 2001241939A AU 4193901 A AU4193901 A AU 4193901A AU 2001241939 A1 AU2001241939 A1 AU 2001241939A1
Authority
AU
Australia
Prior art keywords
nucleic acid
mediated recombination
fragment isolation
stranded nucleic
acid fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001241939A
Inventor
Joseph A Affholter
Brian Carr
Anthony Cox
Jon E. Ness
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen Inc
Original Assignee
Maxygen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc filed Critical Maxygen Inc
Publication of AU2001241939A1 publication Critical patent/AU2001241939A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2001241939A 2000-02-28 2001-02-28 Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation Abandoned AU2001241939A1 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US18524400P 2000-02-28 2000-02-28
US60185244 2000-02-28
US18581500P 2000-02-29 2000-02-29
US60185815 2000-02-29
US18624700P 2000-03-01 2000-03-01
US60186247 2000-03-01
US18648200P 2000-03-02 2000-03-02
US60186482 2000-03-02
US65654900A 2000-09-06 2000-09-06
US09656549 2000-09-06
PCT/US2001/006775 WO2001064864A2 (en) 2000-02-28 2001-02-28 Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation

Publications (1)

Publication Number Publication Date
AU2001241939A1 true AU2001241939A1 (en) 2001-09-12

Family

ID=27539131

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001241939A Abandoned AU2001241939A1 (en) 2000-02-28 2001-02-28 Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation

Country Status (3)

Country Link
US (1) US20060068406A1 (en)
AU (1) AU2001241939A1 (en)
WO (1) WO2001064864A2 (en)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
WO2005012515A2 (en) 2003-04-29 2005-02-10 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US20110111413A1 (en) * 2001-02-02 2011-05-12 Padgett Hal S Method of optimizing codon usage through dna shuffling
US20040152094A1 (en) * 2001-05-10 2004-08-05 Steffen Danielsen Method for producing recombined polynucleotides
EP1281757A1 (en) * 2001-07-31 2003-02-05 Direvo Biotech AG Method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids
AU2003248361B2 (en) * 2002-02-21 2008-07-03 Morphotek, Inc. Methods of making hypermutable cells using PMSR homologs
DK2278509T3 (en) 2002-03-01 2014-12-15 Codexis Mayflower Holdings Llc Methods, systems and software for identification of functional biomolecules
ES2399054T3 (en) * 2004-12-03 2013-03-25 Human Genetic Signatures Pty Ltd Methods to simplify microbial nucleic acids by chemical modification of cytosines
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8431347B2 (en) * 2005-05-26 2013-04-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
EP1943353B1 (en) * 2005-09-14 2011-11-02 Human Genetic Signatures PTY Ltd Assay for a health state
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
CA2647786A1 (en) * 2006-03-14 2007-09-20 Genizon Biosciences Inc. Methods and means for nucleic acid sequencing
US7727745B2 (en) 2006-08-14 2010-06-01 Bio-Id Diagnostic Inc. Synthesis of single-stranded DNA
AU2008329549B2 (en) * 2007-11-27 2013-08-22 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
CA2707165A1 (en) * 2007-12-05 2009-06-11 Human Genetic Signatures Pty Ltd Bisulphite treatment of rna
US20110003700A1 (en) * 2007-12-20 2011-01-06 Human Genetic Signatures Pty Ltd. Elimination of contaminants associated with nucleic acid amplification
WO2009097673A1 (en) * 2008-02-07 2009-08-13 Bio-Id Diagnostic Inc. Synthesis of single-stranded dna
US8768871B2 (en) 2008-02-12 2014-07-01 Codexis, Inc. Method of generating an optimized, diverse population of variants
WO2009143397A2 (en) 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
WO2010028288A2 (en) 2008-09-05 2010-03-11 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
EA027693B1 (en) 2008-09-26 2017-08-31 Токаджен Инк. Recombinant replication competent retrovirus having cytosine deaminase activity for gene therapy of cell proliferative disorders
NZ608313A (en) 2010-09-24 2013-12-20 Univ Leland Stanford Junior Direct capture, amplification and sequencing of target dna using immobilized primers
EP2753710B1 (en) 2011-09-07 2017-01-11 Human Genetic Signatures Pty Ltd Molecular detection assay
WO2014070673A1 (en) 2012-10-29 2014-05-08 University Of Rochester Artemisinin derivatives, methods for their preparation and their use as antimalarial agents
LT2951754T (en) 2013-01-31 2024-04-25 Codexis, Inc. Methods, systems, and software for identifying bio-molecules with interacting components
WO2015100277A2 (en) 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
WO2017075456A1 (en) 2015-10-30 2017-05-04 Beth Israel Deaconess Medical Center, Inc. Methods for determination of bioactivity, quantity, removal, or inactivation of cereal amylase trypsin inhibitors in cereals, flours, plants, and complex foods
US10584363B2 (en) * 2016-06-03 2020-03-10 Takara Bio Usa, Inc. Methods of producing and using single-stranded deoxyribonucleic acids and compositions for use in practicing the same
GB201612011D0 (en) * 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
CA3048939A1 (en) * 2016-12-29 2018-07-05 Johann Wolfgang Goethe-Universitat Frankfurt Am Main Method for generating higher order genome editing libraries
WO2019040335A1 (en) 2017-08-19 2019-02-28 University Of Rochester Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents
GB201721307D0 (en) * 2017-12-19 2018-01-31 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
AU2020267119A1 (en) * 2019-04-30 2021-11-18 Encodia, Inc. Methods for preparing analytes and related kits
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof
WO2023194537A1 (en) * 2022-04-08 2023-10-12 Glaxosmithkline Intellectual Property Development Limited Novel processes for the production of polynucleotides including oligonucleotides

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US5866363A (en) * 1985-08-28 1999-02-02 Pieczenik; George Method and means for sorting and identifying biological information
US5824469A (en) * 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
US5807724A (en) * 1989-08-07 1998-09-15 Resnick; Joseph A. Degradation of petroleum hydrocarbons with organisms encapsulated in wax
WO1991008307A1 (en) * 1989-12-04 1991-06-13 Microprobe Corporation Enhanced capture of target nucleic acid by the use of oligonucleotides covalently attached to polymers
US5358870A (en) * 1990-02-28 1994-10-25 Institute Of Gas Technology Microemulsion process for direct biocatalytic desulfurization of organosulfur molecules
WO1991015581A1 (en) * 1990-04-05 1991-10-17 Roberto Crea Walk-through mutagenesis
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5401632A (en) * 1992-07-16 1995-03-28 Wisconsin Alumni Research Foundation Triple helix purification and sequencing
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5514588A (en) * 1994-12-13 1996-05-07 Exxon Research And Engineering Company Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water
KR100501838B1 (en) * 1995-04-24 2005-07-21 큐비스트 파마슈티컬즈 인코포레이티드 Methods for generating and screening noverl metabolic pathways
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US6004788A (en) * 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US6030779A (en) * 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6168919B1 (en) * 1996-07-17 2001-01-02 Diversa Corporation Screening methods for enzymes and enzyme kits
US5962258A (en) * 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
US5756316A (en) * 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6171820B1 (en) * 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5814473A (en) * 1996-02-09 1998-09-29 Diversa Corporation Transaminases and aminotransferases
US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5962283A (en) * 1995-12-07 1999-10-05 Diversa Corporation Transminases and amnotransferases
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5942430A (en) * 1996-02-16 1999-08-24 Diversa Corporation Esterases
US5958751A (en) * 1996-03-08 1999-09-28 Diversa Corporation α-galactosidase
US6096548A (en) * 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5789228A (en) * 1996-05-22 1998-08-04 Diversa Corporation Endoglucanases
US5877001A (en) * 1996-06-17 1999-03-02 Diverso Corporation Amidase
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US5939300A (en) * 1996-07-03 1999-08-17 Diversa Corporation Catalases
EP0843019A3 (en) * 1996-11-08 2002-10-09 Kyowa Medex Co., Ltd. Method for determination of specific nucleic acid sequence, and a reagent therefor
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US5939261A (en) * 1997-06-24 1999-08-17 Sarnoff Corporation Method for capturing a nucleic acid
US5948666A (en) * 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US5876997A (en) * 1997-08-13 1999-03-02 Diversa Corporation Phytase
IL136574A0 (en) * 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
AU776865B2 (en) * 1998-11-10 2004-09-23 University Of Rochester T cells specific for target antigens and methods and vaccines based thereon
EP1072010B1 (en) * 1999-01-19 2010-04-21 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
WO2001029212A1 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Methods for chimeragenesis of whole genomes or large polynucleotides
WO2001029211A2 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Method for directed evolution by random chimeragenesis on transient templates
IL150291A0 (en) * 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening

Also Published As

Publication number Publication date
WO2001064864A2 (en) 2001-09-07
US20060068406A1 (en) 2006-03-30
WO2001064864A3 (en) 2002-03-28

Similar Documents

Publication Publication Date Title
AU2001241939A1 (en) Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
AU2001279134A1 (en) Nucleotide incorporating enzymes
AU2002245184A1 (en) Nucleic acid amplification
AU2001283151A1 (en) Congestion and thru-put visibility and isolation
AU2002211574A1 (en) Improved guardrail terminals
GB0006733D0 (en) Enzyme
AU2002220553A1 (en) Bioreactor
AU2001288121A1 (en) Enzyme electrode
AU2001255090A1 (en) Cdk inhibitors having 3-hydroxychromen-4-one structure
AU2001245489A1 (en) Human single nucleotide polymorphisms
AU2001232041A1 (en) Improved assay
AU2001285257A1 (en) Human telomerase rna elements
AU2001266689A1 (en) 4-benzyl-2-hydroxy-1,4-oxazin-3-one and polymorphic forms thereof
AU2002215389A1 (en) Optimized messenger rna
AU2001252390A1 (en) Nucleic acid immunization
AU2001292922A1 (en) Nucleic acid detection
AU2001284891A1 (en) Micronized leflunomide
AU2693101A (en) Self-drilling insert
AU2002210587A1 (en) Insulation arrangement
GB0026494D0 (en) Isolation of nucleic acid
AUPQ683400A0 (en) Improved insulation
AUPQ699200A0 (en) Improved insulation
TW408663U (en) Improved screwdriver structure
AUPR223500A0 (en) Nucleic acid sequence
AU2001286579A1 (en) Trunk-boot latch